{"id":12376,"date":"2023-08-24T19:19:50","date_gmt":"2023-08-24T11:19:50","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=12376"},"modified":"2023-08-24T19:19:52","modified_gmt":"2023-08-24T11:19:52","slug":"%e4%b8%80%e7%ab%99%e5%bc%8f%e5%8d%95%e7%bb%86%e8%83%9e%e6%b5%8b%e5%ba%8f%e6%95%b0%e6%8d%ae%e4%b8%8b%e6%b8%b8%e5%88%86%e6%9e%90","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=12376","title":{"rendered":"\u4e00\u7ad9\u5f0f\u5355\u7ec6\u80de\u6d4b\u5e8f\u6570\u636e\u4e0b\u6e38\u5206\u6790"},"content":{"rendered":"\n

http:\/\/www.biocloudservice.com\/366\/366.php<\/a><\/p>\n\n\n\n

<\/p>\n\n\n

1\u3001\u8f6f\u4ef6\u5185\u5bb9\u53ca\u6d41\u7a0b\uff1a<\/h1>\n

\u5171\u5305\u542b1\u4e2aR\u8bed\u8a00\u811a\u672c\uff0c\u811a\u672c\u4ecb\u7ecd\u5982\u4e0b\uff1a<\/p>\n

singcell.R<\/h1>\n

\u7a0b\u5e8f\u540d\u79f0\uff1a<\/h1>\n

singcell.R<\/p>\n

\u529f\u80fd\u63cf\u8ff0\uff1a<\/strong><\/p>\n

\u8be5\u8f6f\u4ef6\u662f\u4e91\u751f\u4fe1\u56e2\u961f\u4e13\u4e3a\u5355\u7ec6\u80de\u6d4b\u5e8f\u5206\u6790\u8bbe\u8ba1\u6253\u9020\uff0c\u53ef\u5b9e\u73b0\u4e00\u7ad9\u5f0f\u5355\u7ec6\u80de\u6d4b\u5e8f\u6570\u636e\u7684\u4e0b\u6e38\u5206\u6790\uff0c\u5305\u62ec\uff1a<\/p>\n

    \n
  1. \u6570\u636e\u8d28\u63a7\u8fc7\u6ee4\uff0c<\/li>\n
  2. \u6570\u636e\u6807\u51c6\u5316\uff0c<\/li>\n
  3. PCA\u4e3b\u6210\u5206\u5206\u6790\uff0c<\/li>\n
  4. tSNE\u964d\u7ef4\u805a\u7c7b\u5206\u6790\uff0c<\/li>\n
  5. \u67e5\u627e\u7ec6\u80de\u7fa4maker\u57fa\u56e0\uff0c<\/li>\n
  6. \u81ea\u52a8\u5316\u6ce8\u91ca\u7ec6\u80de\u7c7b\u578b\uff0c<\/li>\n
  7. \u7ec6\u80de\u8f68\u8ff9\u5206\u6790<\/li>\n<\/ol>\n

    \u7b49\u7b49\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u7684\u79d1\u5b66\u65b9\u6cd5\u3002\u5e76\u4e14\u4f1a\u81ea\u52a8\u751f\u6210\u5206\u6790\u7684\u7ed3\u679c\u56fe\u3002\u5bf9\u7528\u6237\u64cd\u4f5c\u5341\u5206\u53cb\u597d\uff0c\u65e0\u9700\u4efb\u4f55\u7f16\u7801\uff0c\u5373\u53ef\u5feb\u901f\u62ff\u5230\u9ad8\u8d28\u91cf\u7684\u5206\u6790\u7ed3\u679c\u3002<\/p>\n

    \u4f7f\u7528\u65b9\u6cd5\uff1a<\/strong><\/p>\n

    Rscript singcell.R -filename -filepath<\/p>\n

    \u53c2\u6570\u8bf4\u660e\uff1a<\/strong><\/p>\n

    USAGE:<\/p>\n

    singcell.R -filename=<file> -filepath=<path><\/p>\n

    PARAMETERS:<\/p>\n

    -filename The name of the single-cell data folder that needs to be analyzed. Subfolders under the folder may include multiple samples. Under the subfolders should include barcodes,feature, feature_barcode_matrix three single-cell sequencing upstream processed files.<\/p>\n

    -filepath The path to the single-cell data folder that needs to be analyzed.<\/p>\n

      \n
    1. \u6253\u5f00\u547d\u4ee4\u884c\u754c\u9762\uff0c\u8f93\u5165\u201cRscript singcell.R filename filepath\u201d\u8fd9\u91cc\uff0c\u5fc5\u987b\u8f93\u5165\u53c2\u6570\u662f-filename,-filepath;filename\u8868\u793a\u9700\u8981\u8fdb\u884c\u5206\u6790\u7684\u5355\u7ec6\u80de\u6570\u636e\u6587\u4ef6\u5939\u540d\u79f0\u3002\u6587\u4ef6\u5939\u4e0b\u53ef\u4ee5\u5305\u62ec\u591a\u4e2a\u6837\u672c\u7684\u5b50\u6587\u4ef6\u5939\u3002\u5b50\u6587\u4ef6\u5939\u4e0b\u5e94\u5305\u62ecbarcodes,feature\uff0cfeature_barcode_matrix\u4e09\u4e2a\u5355\u7ec6\u80de\u6d4b\u5e8f\u4e0a\u6e38\u5904\u7406\u540e\u7684\u6587\u4ef6;filepath\u8868\u793a\u9700\u8981\u8fdb\u884c\u5206\u6790\u7684\u5355\u7ec6\u80de\u6570\u636e\u6587\u4ef6\u5939\u7684\u8def\u5f84\u3002<\/li>\n<\/ol>\n

      3\u3001\u5b8c\u6210\u53c2\u6570\u63d0\u4ea4\u540e\uff0c\u6309\u4e0b\u56de\u8f66\u952e\uff0c\u6574\u4e2a\u7a0b\u5e8f\u5373\u6b63\u5f0f\u5f00\u59cb\u8fdb\u5165\u6267\u884c\u3002\u7a0b\u5e8f\u6267\u884c\u5b8c\u6bd5\u540e\uff0c\u754c\u9762\u4f1a\u663e\u793a\u201cProgram execution is completed\u201d\u7ed3\u675f\u8bed\u3002<\/p>\n

      \u64cd\u4f5c\u754c\u9762\u622a\u56fe\uff1a<\/strong><\/p>\n

        \n
      1. \u7a0b\u5e8f\u8fd0\u884c\u547d\u4ee4<\/li>\n<\/ol>\n

        <\/p>\n

          \n
        1. \u8f93\u5165\u6587\u4ef6\u754c\u9762\u5c55\u793a\u622a\u56fe\u793a\u4f8b<\/li>\n<\/ol>\n

          \u793a\u4f8b\u8f93\u5165\u7684\u6587\u4ef6GSE174574_RAW\/ \u4e0b\u6709\u4e09\u4e2a\u6837\u672c<\/p>\n

          <\/p>\n

          \u6837\u672c\u6587\u4ef6\u5939\u4e0b\uff0c\u5e94\u5305\u62ecbarcodes, feature, feature_barcode_matrix\u4e09\u4e2a\u5355\u7ec6\u80de\u6d4b\u5e8f\u4e0a\u6e38\u5904\u7406\u540e\u7684\u6587\u4ef6\u3002<\/p>\n

          <\/p>\n

            \n
          1. \u7a0b\u5e8f\u8fd0\u884c\u754c\u9762\u622a\u56fe<\/li>\n<\/ol>\n

            <\/p>\n

              \n
            1. \u7a0b\u5e8f\u8fd0\u884c\u7ed3\u679c\u6587\u4ef6\u5c55\u793a<\/li>\n<\/ol>\n

              <\/p>\n

              \u8f93\u51fa\u6587\u4ef6\uff1a<\/strong><\/p>\n

              \u7ed3\u679c\u6587\u4ef6\u5206\u4e3a\u4e94\u4e2a\u90e8\u5206\uff0c\u5206\u522b\u662f\uff1a<\/p>\n

                \n
              1. \u57fa\u56e0\u7279\u5f81<\/li>\n
              2. PCA\u5206\u6790<\/li>\n
              3. Maker\u57fa\u56e0\u8bc6\u522b<\/li>\n
              4. tSNE\u805a\u7c7b<\/li>\n
              5. \u7ec6\u80de\u8f68\u8ff9\u56fe<\/li>\n<\/ol>\n

                \u8f93\u51fa\u7684\u56fe\u7247\u5c06\u4ee5pdf\u683c\u5f0f\u4fdd\u5b58\u3002\u5206\u6790\u6570\u636e\u4ee5txt\u683c\u5f0f\u4fdd\u5b58\u3002<\/p>\n

                  \n
                1. \u6d4b\u5e8f\u6df1\u5ea6\u76f8\u5173\u6027\u56fe<\/li>\n<\/ol>\n

                  <\/p>\n

                    \n
                  1. \u7279\u5f81\u65b9\u5dee\u56fe <\/li>\n
                  2. \u5355\u7ec6\u80de\u5206\u5e03\u5c0f\u63d0\u7434\u56fe <\/li>\n
                  3. PCA\u5206\u6790\u70ed\u56fe <\/li>\n
                  4. FeaturePlot<\/li>\n
                  5. <\/li>\n
                  6. tSNE\u6295\u5f71\u56fe <\/li>\n
                  7. \u8bc6\u522b\u7ec6\u80de\u7c7b\u578b <\/li>\n
                  8. 03.clusterMarkers.txt<\/li>\n<\/ol>\n

                    <\/p>\n

                      \n
                    1. 04.cellAnn.txt<\/li>\n<\/ol>\n

                      <\/p>\n

                      \u7b49\u6587\u4ef6<\/p>\n

                      \u4e91\u5e73\u53f0\u4e0b\u64cd\u4f5c\u622a\u56fe\uff1a<\/strong><\/p>\n

                      (1)\u8fd0\u884c\u793a\u4f8b\u6570\u636e<\/p>\n

                      <\/p>\n

                      \u53ef\u5f97\u5230\u793a\u4f8b\u6570\u636e\u7684\u7ed3\u679c<\/p>\n

                      <\/p>\n

                      \u70b9\u51fb\u4e0b\u8f7d\u5168\u90e8\u6587\u4ef6\uff0c\u5373\u53ef\u4e0b\u8f7d\u5168\u90e8\u7684\u5206\u6790\u7ed3\u679c\u6587\u4ef6\u3002<\/p>\n

                        \n
                      1. \u4e0a\u4f20\u6570\u636e<\/li>\n<\/ol>\n

                        <\/p>\n

                        \u70b9\u51fb\u4e0a\u4f20\u6570\u636e<\/p>\n

                        <\/p>\n

                        \u5373\u53ef\u8fdb\u5165\u6587\u4ef6\u4e0a\u4f20\u7684\u9875\u9762\u3002\u9009\u62e9\u6211\u4eec\u8981\u5206\u6790\u7684\u6570\u636e<\/p>\n

                        <\/p>\n

                        \u4e0a\u4f20\u597d\u540e\uff0c\u70b9\u51fb\u8fd0\u884c\u81ea\u6709\u6570\u636e\uff0c\u5373\u53ef\u5f00\u59cb\u5206\u6790\u3002<\/p>\n

                        \u7a0b\u5e8f\u8fd0\u884c\u5b8c\u6210\u540e\uff0c\u5c06\u4f1a\u8f93\u51fa\u4ee5\u4e0b\u7ed3\u679c\u3002<\/p>\n

                        <\/p>","protected":false},"excerpt":{"rendered":"

                        http:\/\/www.biocloudservice.com\/366\/366.php 1\u3001\u8f6f\u4ef6\u5185\u5bb9\u53ca\u6d41\u7a0b\uff1a \u5171 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/12376"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=12376"}],"version-history":[{"count":2,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/12376\/revisions"}],"predecessor-version":[{"id":12398,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/12376\/revisions\/12398"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=12376"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=12376"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=12376"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}