{"id":1497,"date":"2023-01-19T14:43:59","date_gmt":"2023-01-19T06:43:59","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=1497"},"modified":"2023-01-19T14:43:59","modified_gmt":"2023-01-19T06:43:59","slug":"t-sp1-a-novel-serine-protease-like-protein-predominantly-expressed-in-testis","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=1497","title":{"rendered":"T-SP1: a novel serine protease-like protein predominantly expressed in testis."},"content":{"rendered":"

Research |Published:2009-2-3\u00a0 | ISSN:1431-6730\u00a0 |doi:10.1515\/BC.2008.170 |pmid:18844450<\/p>\n

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Abstract<\/h2>\n
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Here, we describe a novel member in the group of membrane-anchored chymotrypsin (S1)-like serine proteases, namely testis serine protease 1 (T-SP1), as it is principally expressed in testis tissue. The human T-SP1 gene encompasses 28.7 kb on the short arm of chromosome 8 and consists of seven exons. Rapid amplification of cDNA ends (RACE) experiments revealed that due to alternative splicing three different variants (T-SP1\/1, -2, -3) are detectable in testis tissue displaying pronounced heterogeneity at their 3′-end. T-SP1\/1 consists of an 18 amino acid signal peptide and of a 49 amino acid propeptide. The following domain with the catalytic triad of His(108), Asp(156), and Ser(250) shares sequence identities of 42% and 40% with the blood coagulation factor XI and plasma kallikrein, respectively. Only T-SP1\/1 contains a hydrophobic part at the C-terminus, which provides the basis for cell membrane anchoring. Using a newly generated polyclonal anti-T-SP1 antibody, expression of the T-SP1 protein was found in the Leydig and Sertoli cells of the testis and in the epithelial cells of the ductuli efferentes. Notably, T-SP1 protein was also detectable in prostate cancer and in some ovarian cancer tissues, indicating tumor-related synthesis of T-SP1 beyond testis tissue.<\/p>\n

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T-SP1:\u4e00\u79cd\u4e3b\u8981\u5728\u777e\u4e38\u4e2d\u8868\u8fbe\u7684\u65b0\u578b\u4e1d\u6c28\u9178\u86cb\u767d\u9176\u6837\u86cb\u767d\u3002<\/h2>\n
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\u8fd9\u91cc\uff0c\u6211\u4eec\u63cf\u8ff0\u4e86\u819c\u951a\u5b9a\u80f0\u51dd\u4e73\u86cb\u767d\u9176(S1)\u6837\u4e1d\u6c28\u9178\u86cb\u767d\u9176\u7ec4\u4e2d\u7684\u4e00\u4e2a\u65b0\u6210\u5458\uff0c\u5373\u777e\u4e38\u4e1d\u6c28\u9178\u86cb\u767d\u91761 (T-SP1)\uff0c\u56e0\u4e3a\u5b83\u4e3b\u8981\u5728\u777e\u4e38\u7ec4\u7ec7\u4e2d\u8868\u8fbe\u3002\u4eba\u7c7bT-SP1\u57fa\u56e0\u5305\u542b8\u53f7\u67d3\u8272\u4f53\u77ed\u81c2\u4e0a\u768428.7 kb\uff0c\u75317\u4e2a\u5916\u663e\u5b50\u7ec4\u6210\u3002cDNA\u672b\u7aef\u5feb\u901f\u6269\u589e(RACE)\u5b9e\u9a8c\u8868\u660e\uff0c\u7531\u4e8e\u9009\u62e9\u6027\u526a\u63a5\uff0c\u5728\u777e\u4e38\u7ec4\u7ec7\u4e2d\u53ef\u68c0\u6d4b\u5230\u4e09\u79cd\u4e0d\u540c\u7684\u53d8\u4f53(T-SP1\/1\uff0c-2\uff0c-3)\uff0c\u5728\u51763\u2019-\u672b\u7aef\u663e\u793a\u51fa\u660e\u663e\u7684\u5f02\u8d28\u6027\u3002T-SP1\/1\u753118\u4e2a\u6c28\u57fa\u9178\u7684\u4fe1\u53f7\u80bd\u548c49\u4e2a\u6c28\u57fa\u9178\u7684\u524d\u80bd\u7ec4\u6210\u3002\u5177\u6709His(108)\u3001Asp(156)\u548cSer(250)\u50ac\u5316\u4e09\u8054\u4f53\u7684\u4e0b\u5217\u7ed3\u6784\u57df\u5206\u522b\u4e0e\u51dd\u8840\u56e0\u5b50XI\u548c\u8840\u6d46\u6fc0\u80bd\u91ca\u653e\u9176\u5177\u670942%\u548c40%\u7684\u5e8f\u5217\u540c\u4e00\u6027\u3002\u53ea\u6709T-SP1\/1\u5728C\u672b\u7aef\u542b\u6709\u758f\u6c34\u90e8\u5206\uff0c\u8fd9\u4e3a\u7ec6\u80de\u819c\u951a\u5b9a\u63d0\u4f9b\u4e86\u57fa\u7840\u3002\u4f7f\u7528\u65b0\u4ea7\u751f\u7684\u591a\u514b\u9686\u6297T-SP1\u6297\u4f53\uff0c\u53d1\u73b0T-SP1\u86cb\u767d\u5728\u777e\u4e38\u95f4\u8d28\u7ec6\u80de\u548c\u652f\u6301\u7ec6\u80de\u4ee5\u53ca\u8f93\u51fa\u5c0f\u7ba1\u4e0a\u76ae\u7ec6\u80de\u4e2d\u8868\u8fbe\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0cT-SP1\u86cb\u767d\u5728\u524d\u5217\u817a\u764c\u548c\u4e00\u4e9b\u5375\u5de2\u764c\u7ec4\u7ec7\u4e2d\u4e5f\u53ef\u68c0\u6d4b\u5230\uff0c\u8fd9\u8868\u660eT-SP1\u7684\u80bf\u7624\u76f8\u5173\u5408\u6210\u8d85\u51fa\u4e86\u777e\u4e38\u7ec4\u7ec7\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Research |Published:2009-2-3\u00a0 | ISSN:1431-6730\u00a0 |doi:10 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1497"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1497"}],"version-history":[{"count":0,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1497\/revisions"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1497"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1497"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1497"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}