{"id":1500,"date":"2023-01-19T14:43:59","date_gmt":"2023-01-19T06:43:59","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=1500"},"modified":"2023-01-19T14:43:59","modified_gmt":"2023-01-19T06:43:59","slug":"stabilization-of-g-domain-conformations-in-the-trna-modifying-mnme-gida-complex-observed-with-double-electron-electron-resonance-spectroscopy","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=1500","title":{"rendered":"Stabilization of G domain conformations in the tRNA-modifying MnmE-GidA complex observed with double electron electron resonance spectroscopy."},"content":{"rendered":"<p>Research |Published:2010-6-18\u00a0 | ISSN:0021-9258\u00a0 |doi:10.1074\/jbc.M109.096131 |pmid:20353943<\/p>\n<hr \/>\n<h2 id=\"Abs1\" class=\"c-article-section__title js-section-title js-c-reading-companion-sections-item\">Abstract<\/h2>\n<hr \/>\n<div id=\"Abs1-content\" class=\"c-article-section__content\">\n<p>MnmE is a GTP-binding protein conserved between bacteria and eukarya. It is a dimeric three-domain protein where the two G domains have to approach each other for activation of the potassium-stimulated GTPase reaction. Together with GidA, in a heterotetrameric alpha(2)beta(2) complex, it is involved in the modification of the wobble uridine base U34 of the first anticodon position of particular tRNAs. Here we show, using various spin-labeled MnmE mutants and EPR spectroscopy, that GidA binding induces large conformational and dynamic changes in MnmE. It stimulates the GTPase reaction by stabilizing the GTP-bound conformation in a potassium-independent manner. Surprisingly, GidA binding influences not only the GTP- but also the GDP-bound conformation. Thus GidA is a new type of regulator for a G protein activated by dimerization.<\/p>\n<hr \/>\n<h2 id=\"Abs1\" class=\"c-article-section__title js-section-title js-c-reading-companion-sections-item\">\u7528\u53cc\u7535\u5b50\u7535\u5b50\u5171\u632f\u5149\u8c31\u6cd5\u89c2\u5bdftRNA\u4fee\u9970\u7684MnmE-GidA\u590d\u5408\u7269\u4e2dG\u7ed3\u6784\u57df\u6784\u8c61\u7684\u7a33\u5b9a\u6027\u3002<\/h2>\n<hr \/>\n<p>MnmE\u662f\u7ec6\u83cc\u548c\u771f\u6838\u751f\u7269\u4e4b\u95f4\u4fdd\u5b88\u7684GTP\u7ed3\u5408\u86cb\u767d\u3002\u5b83\u662f\u4e00\u79cd\u4e8c\u805a\u4f53\u4e09\u7ed3\u6784\u57df\u86cb\u767d\u8d28\uff0c\u5176\u4e2d\u4e24\u4e2aG\u7ed3\u6784\u57df\u5fc5\u987b\u76f8\u4e92\u9760\u8fd1\u4ee5\u6fc0\u6d3b\u94be\u523a\u6fc0\u7684GTP\u9176\u53cd\u5e94\u3002\u5728\u5f02\u56db\u805a\u03b1(2)\u03b2(2)\u590d\u5408\u7269\u4e2d\uff0c\u5b83\u4e0eGidA\u4e00\u8d77\u53c2\u4e0e\u7279\u5b9atRNAs\u7b2c\u4e00\u4e2a\u53cd\u5bc6\u7801\u5b50\u4f4d\u7f6e\u7684\u6446\u52a8\u5c3f\u82f7\u78b1\u57faU34\u7684\u4fee\u9970\u3002\u5728\u8fd9\u91cc\uff0c\u6211\u4eec\u4f7f\u7528\u5404\u79cd\u81ea\u65cb\u6807\u8bb0\u7684MnmE\u7a81\u53d8\u4f53\u548c\u7535\u5b50\u987a\u78c1\u5171\u632f\u6ce2\u8c31\u663e\u793a\uff0cGidA\u7ed3\u5408\u8bf1\u5bfcMnmE\u5927\u7684\u6784\u8c61\u548c\u52a8\u6001\u53d8\u5316\u3002\u5b83\u901a\u8fc7\u4ee5\u4e0d\u4f9d\u8d56\u94be\u7684\u65b9\u5f0f\u7a33\u5b9aGTP\u7ed3\u5408\u6784\u8c61\u6765\u523a\u6fc0GTP\u9176\u53cd\u5e94\u3002\u4ee4\u4eba\u60ca\u8bb6\u7684\u662f\uff0cGidA\u7ed3\u5408\u4e0d\u4ec5\u5f71\u54cdGTP\uff0c\u800c\u4e14\u5f71\u54cdGDP\u7ed3\u5408\u6784\u8c61\u3002\u56e0\u6b64GidA\u662f\u4e00\u79cd\u65b0\u578b\u7684\u7531\u4e8c\u805a\u5316\u6fc0\u6d3b\u7684G\u86cb\u767d\u8c03\u8282\u5242\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Research |Published:2010-6-18\u00a0 | ISSN:0021-9258\u00a0 |doi:1 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1500"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1500"}],"version-history":[{"count":0,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1500\/revisions"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1500"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1500"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1500"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}