{"id":23890,"date":"2024-01-09T17:33:13","date_gmt":"2024-01-09T09:33:13","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=23890"},"modified":"2024-01-09T17:33:15","modified_gmt":"2024-01-09T09:33:15","slug":"mega-%e5%ba%8f%e5%88%97%e6%af%94%e5%af%b9%ef%bc%8c%e7%bb%98%e5%88%b6%e5%8f%91%e8%82%b2%e6%a0%91","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=23890","title":{"rendered":"MEGA\u2014\u2014\u5e8f\u5217\u6bd4\u5bf9\uff0c\u7ed8\u5236\u53d1\u80b2\u6811"},"content":{"rendered":"

MEGA\u662f\u591a\u5e8f\u5217\u6bd4\u5bf9\u7684\u8f6f\u4ef6\uff0c\u5e76\u4e14\u53ef\u4ee5\u8fdb\u884c\u7ed8\u5236\u7cfb\u7edf\u53d1\u80b2\u6811\u3002\u81ea1993\u5e74\u53d1\u5e03\u4ee5\u6765\uff0cMEGA\u5171\u66f4\u65b09\u4e2a\u7248\u672c (\u6ca1\u6709\u7b2c\u516b\u3001\u4e5d\u7248)\uff0c\u4eca\u5e74\u53d1\u5e03\u7684MEGA 11\u4e3a\u5904\u7406\u66f4\u5927\u7684\u6570\u636e\u96c6\u8fdb\u884c\u4e86\u4f18\u5316\u3002<\/p>\n

\"\u622a\u5c4f2023-06-13<\/p>\n

\u8f6f\u4ef6\u7684\u4e0b\u8f7d\uff1a<\/h2>\n

\u6839\u636e\u4f7f\u7528\u7535\u8111\u7cfb\u7edf\u7684\u60c5\u51b5\uff0c\u53ef\u652f\u6301Win\u3001Mac\u548cLinux\u7cfb\u7edf\u3002\u4e0b\u8f7d\u94fe\u63a5\u5982\u4e0b\uff1ahttps:\/\/www.megasoftware.net\/<\/p>\n

\"\u622a\u5c4f2023-06-13 \u4e0b\u8f7d\u597d\u7684\u8f6f\u4ef6\u56fe\u6807<\/p>\n

\u57fa\u672c\u64cd\u4f5c<\/h2>\n

\u6570\u636e\u5bfc\u5165<\/h3>\n

\u4e0b\u8f7d\u5b89\u88c5\u597d MEGA 11 \u540e\uff0c\u9996\u5148\u6253\u5f00\u8f6f\u4ef6\u3002<\/p>\n

\"IMG_256\"<\/p>\n

\u5728 MEGA 11\u9996\u9875\u9009\u62e9”ALIGN”\uff0c\u70b9\u51fb “Edit\/Build Alignment” \uff0c\u4f1a\u5f39\u51fa\u4e00\u4e2a\u5bf9\u8bdd\u6846\uff0c\u9009\u62e9”Create a new alignment”\uff0c\u6839\u636e\u9700\u8981\u6bd4\u5bf9\u7684\u5e8f\u5217 (\u6c28\u57fa\u9178\u5e8f\u5217\u6216\u6838\u82f7\u9178\u5e8f\u5217)\uff0c\u9009\u62e9”DNA”\u6216”Protein”\u3002\uff08\u5e8f\u5217\u53ef\u4ee5\u5728NCBI\u4e0b\u8f7d\u4e00\u4e2a\uff09<\/p>\n

\u70b9\u51fb”Edit”\uff0c\u9009\u62e9”Insert Sequence From File”\u5bfc\u5165\u6211\u4eec\u9700\u8981\u6bd4\u5bf9\u7684\u5e8f\u5217\uff0c\u5e8f\u5217\u6587\u4ef6\u683c\u5f0f\u4e3a.fasta\u683c\u5f0f\u7684\u54e6\uff5e<\/p>\n

\"IMG_257\"<\/p>\n

\"IMG_258\"<\/p>\n

\"\u622a\u5c4f2023-06-13<\/p>\n

\u5e8f\u5217\u6bd4\u5bf9<\/h3>\n

\"\u622a\u5c4f2023-06-13<\/p>\n

\u5bf9\u76ee\u6807\u5e8f\u5217\u8fdb\u884c\u591a\u5e8f\u5217\u6bd4\u8f83\uff0c\u53ef\u4ee5\u4f7f\u7528ClustalW\u548cMUSCLE\uff0c\u8fd9\u91cc\u9009\u62e9\u6839\u636e\u4e2a\u4eba\u559c\u597d\u548c\u67e5\u9605\u76f8\u5173\u7684\u6587\u4ef6\u786e\u5b9a\u7528\u4ec0\u4e48\u7b97\u6cd5\u8fdb\u884c\u6bd4\u5bf9\u3002<\/p>\n

\u6bd4\u8f83\u7ed3\u679c\u5982\u56fe\u6240\u793a\uff1a<\/p>\n

\"\u622a\u5c4f2023-06-13 \u6700\u540e\u70b9\u51fb”Data”\u9009\u62e9”Save Session”\uff0c\u4fdd\u5b58\u5e8f\u5217\u6bd4\u5bf9\u7684\u7ed3\u679c\u3002<\/p>\n

\"IMG_264\"<\/p>\n

\u7cfb\u7edf\u53d1\u80b2\u6811<\/h3>\n

MEGA\u53ef\u4ee5\u57fa\u4e8e\u5e8f\u5217\u6bd4\u5bf9\u7684\u7ed3\u679c\u8fdb\u884c\u8fdb\u4e00\u6b65\u7ed8\u5236\u53d1\u80b2\u6811\u3002\u70b9\u51fb”Data”\uff0c\u9009\u62e9”Phylogenetic Analysis”\u8fdb\u884c\u7cfb\u7edf\u53d1\u80b2\u5206\u6790\u3002<\/p>\n

\"IMG_267\"<\/p>\n

\u7136\u540e\uff0c\u8fd4\u56de\u4e3b\u9875\u9762\uff0c\u70b9\u51fb”PHYLOGENY”\uff0c\u6784\u5efa\u7cfb\u7edf\u53d1\u80b2\u6811\u4e3b\u8981\u6709\u4e09\u79cd\u65b9\u6cd5\uff0c\u5206\u522b\u662f\u6700\u5927\u4f3c\u7136\u6cd5 (Maximum Likelihood)\u3001\u90bb\u63a5\u6cd5 (Neighbor-Joining) \u548c\u6700\u5c0f\u8fdb\u5316\u6cd5 (Minimum Evolution)\u3002<\/p>\n

\"\u622a\u5c4f2023-06-13<\/p>\n

\u8fd9\u91cc\u8865\u5145\u4e00\u4e0b\u6709\u5173\u7684\u7b97\u6cd5\u7684\u4ecb\u7ecd\uff1a<\/p>\n

\u5728\u7cfb\u7edf\u8fdb\u5316\u5b66\u4e2d\uff0c\u5e38\u7528\u7684\u6811\u6784\u5efa\u65b9\u6cd5\u5305\u62ec\u6700\u5927\u7b80\u7ea6\u6cd5\uff08Maximum Parsimony, MP\uff09\u3001\u6700\u5927\u4f3c\u7136\u6cd5\uff08Maximum Likelihood, ML\uff09\u3001\u8d1d\u53f6\u65af\u65b9\u6cd5\uff08Bayesian Inference, BI\uff09\u548c\u90bb\u63a5\u6cd5\uff08Neighbor Joining, NJ\uff09\u7b49\u3002\u8fd9\u4e9b\u65b9\u6cd5\u90fd\u6709\u5404\u81ea\u7684\u4f18\u70b9\u548c\u5c40\u9650\u6027\uff0c\u4e0b\u9762\u7b80\u5355\u6bd4\u8f83\u4e00\u4e0b\u5b83\u4eec\u7684\u7279\u70b9\uff1a<\/p>\n

\u6700\u5927\u7b80\u7ea6\u6cd5\uff08MP\uff09\uff1aMP\u662f\u4e00\u79cd\u57fa\u4e8e\u5b57\u7b26\u5206\u6790\u7684\u6811\u6784\u5efa\u65b9\u6cd5\uff0c\u5b83\u5047\u8bbe\u5728\u8fdb\u5316\u8fc7\u7a0b\u4e2d\u5c3d\u91cf\u51cf\u5c11\u8fdb\u5316\u4e8b\u4ef6\u7684\u6570\u91cf\u3002\u56e0\u6b64\uff0cMP\u65b9\u6cd5\u7684\u4f18\u70b9\u662f\u8ba1\u7b97\u901f\u5ea6\u5feb\uff0c\u9002\u7528\u4e8e\u5c0f\u6837\u672c\u548c\u5c0f\u6570\u636e\u96c6\u3002\u7f3a\u70b9\u662f\u5bf9\u6570\u636e\u7684\u7f3a\u5931\u548c\u591a\u6001\u6027\u8f83\u654f\u611f\uff0c\u800c\u4e14\u5728\u590d\u6742\u6a21\u578b\u4e2d\u7684\u5e94\u7528\u6548\u679c\u4e0d\u5982\u5176\u4ed6\u65b9\u6cd5\u3002<\/p>\n

\u6700\u5927\u4f3c\u7136\u6cd5\uff08ML\uff09\uff1aML\u662f\u4e00\u79cd\u57fa\u4e8e\u6a21\u578b\u7684\u6811\u6784\u5efa\u65b9\u6cd5\uff0c\u5b83\u5229\u7528\u6982\u7387\u6a21\u578b\u8ba1\u7b97\u6570\u636e\u7684\u4f3c\u7136\u5ea6\uff0c\u5bfb\u627e\u6700\u6709\u53ef\u80fd\u4ea7\u751f\u89c2\u6d4b\u6570\u636e\u7684\u6811\u3002ML\u65b9\u6cd5\u7684\u4f18\u70b9\u662f\u5bf9\u6570\u636e\u7684\u7f3a\u5931\u548c\u591a\u6001\u6027\u8f83\u4e3a\u9c81\u68d2\uff0c\u800c\u4e14\u5728\u590d\u6742\u6a21\u578b\u4e2d\u6709\u8f83\u597d\u7684\u8868\u73b0\u3002\u7f3a\u70b9\u662f\u8ba1\u7b97\u590d\u6742\u5ea6\u9ad8\uff0c\u9700\u8981\u8f83\u957f\u7684\u8fd0\u884c\u65f6\u95f4\uff0c\u4e14\u5bf9\u4e8e\u5927\u6570\u636e\u96c6\u548c\u590d\u6742\u6a21\u578b\u6765\u8bf4\uff0c\u53ef\u80fd\u4f1a\u51fa\u73b0\u8fc7\u62df\u5408\u7684\u60c5\u51b5\u3002<\/p>\n

\u8d1d\u53f6\u65af\u65b9\u6cd5\uff08BI\uff09\uff1aBI\u662f\u4e00\u79cd\u57fa\u4e8e\u6982\u7387\u7684\u6811\u6784\u5efa\u65b9\u6cd5\uff0c\u5b83\u4f7f\u7528\u8d1d\u53f6\u65af\u7edf\u8ba1\u5b66\u539f\u7406\u4f30\u8ba1\u6811\u7684\u540e\u9a8c\u6982\u7387\u3002BI\u65b9\u6cd5\u7684\u4f18\u70b9\u662f\u53ef\u4ee5\u6709\u6548\u5904\u7406\u591a\u6001\u6027\u548c\u7f3a\u5931\u6570\u636e\uff0c\u5e76\u4e14\u53ef\u4ee5\u63d0\u4f9b\u6811\u7684\u7f6e\u4fe1\u5ea6\u3002\u7f3a\u70b9\u662f\u8ba1\u7b97\u590d\u6742\u5ea6\u8f83\u9ad8\uff0c\u9700\u8981\u957f\u65f6\u95f4\u7684\u8fd0\u884c\uff0c\u540c\u65f6\u4e5f\u9700\u8981\u9009\u62e9\u5408\u9002\u7684\u5148\u9a8c\u6982\u7387\u5206\u5e03\u3002<\/p>\n

\u90bb\u63a5\u6cd5\uff08NJ\uff09\uff1aNJ\u662f\u4e00\u79cd\u57fa\u4e8e\u8ddd\u79bb\u7684\u6811\u6784\u5efa\u65b9\u6cd5\uff0c\u5b83\u5229\u7528\u5e8f\u5217\u4e4b\u95f4\u7684\u8ddd\u79bb\u8ba1\u7b97\u6811\u7684\u62d3\u6251\u7ed3\u6784\u3002NJ\u65b9\u6cd5\u7684\u4f18\u70b9\u662f\u8ba1\u7b97\u901f\u5ea6\u5feb\u3001\u7a33\u5b9a\u6027\u9ad8\u3001\u9002\u7528\u4e8e\u5927\u6837\u672c\u548c\u5927\u6570\u636e\u96c6\u3002\u7f3a\u70b9\u662f\u5bf9\u4e8e\u8ddd\u79bb\u77e9\u9635\u7684\u9519\u8bef\u6216\u504f\u5dee\u6bd4\u8f83\u654f\u611f\uff0c\u56e0\u6b64\u9700\u8981\u5408\u7406\u9009\u62e9\u8ddd\u79bb\u8ba1\u7b97\u65b9\u6cd5\u3002<\/p>\n

\u9700\u8981\u6ce8\u610f\u7684\u662f\uff0c\u4e0d\u540c\u7684\u6811\u6784\u5efa\u65b9\u6cd5\u53ef\u80fd\u4f1a\u4ea7\u751f\u4e0d\u540c\u7684\u6811\u7ed3\u6784\u548c\u5206\u652f\u957f\u5ea6\uff0c\u56e0\u6b64\u9009\u62e9\u5408\u9002\u7684\u65b9\u6cd5\u9700\u8981\u6839\u636e\u5b9e\u9645\u60c5\u51b5\u6765\u786e\u5b9a\u3002\u540c\u65f6\uff0c\u591a\u79cd\u65b9\u6cd5\u7ed3\u5408\u4f7f\u7528\u6216\u5bf9\u6bd4\u5206\u6790\u53ef\u4ee5\u66f4\u597d\u5730\u63d0\u9ad8\u5206\u6790\u7ed3\u679c\u7684\u51c6\u786e\u6027\u3002<\/p>\n

\u4e00\u822c\u6211\u4eec\u5728MEGA\u4e2d\u9009\u62e9\u7b2c\u4e8c\u4e2a\u90bb\u63a5\u6cd5\uff08Neighbor Joining, NJ\uff09\u6765\u7ed8\u5236\u7cfb\u7edf\u53d1\u80b2\u6811\uff0c\u8bbe\u7f6e\u53c2\u6570\u8bbe\u7f6e\uff0cBootstrap method\uff0c1000\u6b21\u3002<\/strong><\/p>\n

\"\/private\/var\/folders\/xd\/jn2f48nj2k52zzgd8wm1j4yr0000gn\/T\/com.kingsoft.wpsoffice.mac\/photoedit2\/20230613180008\/temp.pngtemp\"<\/p>\n

\u7b49\u5f85\u5b8c\u6210\u540e\uff0c\u53ef\u4ee5\u5728\u8fd9\u4e2a\u9875\u9762\u8fdb\u884c\u4fee\u6539\u4e00\u4e9b\u53c2\u6570\uff0c<\/p>\n

\"\u622a\u5c4f2023-06-13<\/p>\n

\u4fdd\u5b58<\/h3>\n

\u70b9\u51fb”Copy to Clipboard”\u53ef\u4fdd\u5b58\u4e3a\u591a\u79cd\u56fe\u7247\u683c\u5f0f (PDF\u3001PNG\u3001TIFF\u7b49)\uff0c\u8fd8\u53ef\u4ee5\u9009\u62e9”Copy to Clipboad”\uff0c\u76f4\u63a5\u590d\u5236\u5230word\u4e2d\u8fdb\u884c\u56fe\u7247\u7f16\u8f91(wps\u4e0d\u53ef\u4ee5)\u3002<\/p>\n

\"IMG_256\"<\/p>\n

\u8fd9\u6837\u6211\u4eec\u5c31\u5b66\u4f1a\u4e86\u7528MEGA\u8fd9\u4e2a\u8f6f\u4ef6\u5566\uff0c\u5c0f\u4f19\u4f34\u4eec\u5982\u679c\u6709\u4ec0\u4e48\u95ee\u9898\u5c31\u548c\u5c0f\u679c\u8ba8\u8bba\u5427\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

MEGA\u662f\u591a\u5e8f\u5217\u6bd4\u5bf9\u7684\u8f6f\u4ef6\uff0c\u5e76\u4e14\u53ef\u4ee5\u8fdb\u884c\u7ed8\u5236\u7cfb\u7edf\u53d1\u80b2\u6811\u3002\u81ea1993\u5e74\u53d1\u5e03\u4ee5\u6765\uff0cMEGA\u5171\u66f4\u65b09\u4e2a\u7248\u672c (\u6ca1\u6709\u7b2c […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/23890"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=23890"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/23890\/revisions"}],"predecessor-version":[{"id":23905,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/23890\/revisions\/23905"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=23890"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=23890"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=23890"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}