{"id":25269,"date":"2024-01-19T16:59:30","date_gmt":"2024-01-19T08:59:30","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=25269"},"modified":"2024-01-19T16:59:31","modified_gmt":"2024-01-19T08:59:31","slug":"%e4%b8%80%e5%88%86%e9%92%9f%e5%ad%a6%e4%bc%9a%e5%a6%82%e4%bd%95%e5%a4%84%e7%90%86fastq%e6%96%87%e4%bb%b6","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=25269","title":{"rendered":"\u4e00\u5206\u949f\u5b66\u4f1a\u5982\u4f55\u5904\u7406FASTQ\u6587\u4ef6"},"content":{"rendered":"

<\/h1>\n

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\u4ec0\u4e48\u662fFASTQ\u6587\u4ef6\uff1f<\/h2>\n

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    \n
  1. \u7b2c\u4e00\u884c\u4ee5\u7b26\u53f7 “@” \u5f00\u5934\uff0c\u540e\u63a5\u4e00\u4e2a\u552f\u4e00\u7684\u6807\u8bc6\u7b26\uff0c\u8868\u793a\u8be5\u5e8f\u5217\u7684\u540d\u79f0\u6216\u7f16\u53f7\u3002<\/li>\n
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  3. \u7b2c\u4e09\u884c\u4ee5\u7b26\u53f7 “+” \u5f00\u5934\uff0c\u901a\u5e38\u8ddf\u968f\u7740\u4e0e\u7b2c\u4e00\u884c\u76f8\u540c\u7684\u6807\u8bc6\u7b26\uff0c\u7528\u4e8e\u53ef\u9009\u7684\u63cf\u8ff0\u6027\u4fe1\u606f\uff08\u5728\u4e00\u4e9b\u60c5\u51b5\u4e0b\u53ef\u4ee5\u5ffd\u7565\uff09\u65e7\u7248FASTQ\u683c\u5f0f\u4e2d\u4f1a\u5728\u8fd9\u4e00\u884c\u91cd\u590d\u7b2c\u4e00\u884c\u4fe1\u606f\uff0c\u73b0\u5728\u4e3a\u8282\u7ea6\u5b58\u50a8\uff0c\u4e00\u822c\u662f\u4e0d\u518d\u52a0\u91cd\u590d\u4fe1\u606f\u3002<\/li>\n
  4. \u7b2c\u56db\u884c\u662f\u4e0e\u7b2c\u4e8c\u884c\u5bf9\u5e94\u7684\u8d28\u91cf\u503c\u5e8f\u5217\uff0c\u8fd9\u4e00\u884c\u4fe1\u606f\u81f3\u5173\u91cd\u8981<\/strong>\uff0c\u901a\u5e38\u4f7f\u7528ASCII\u7f16\u7801\u8868\u793a\uff0c\u6bcf\u4e2a\u5b57\u7b26\u5bf9\u5e94\u4e00\u4e2a\u6d4b\u5e8f\u7247\u6bb5\u4e2d\u5bf9\u5e94\u4f4d\u7f6e\u7684\u8d28\u91cf\u503c\u3002<\/li>\n<\/ol>\n

    <\/p>\n

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    \u4ec0\u4e48\u662f\u8d28\u91cf\u503cQ\u548c\u8d28\u91cf\u5206\u6570Phred\uff1f<\/h2>\n

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    <\/p>\n

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    <\/p>\n

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    \u90a3\u4e48FASTA\u6587\u4ef6\u53c8\u53ef\u4ee5\u5982\u4f55\u63a5\u7740\u5904\u7406\u5462\uff1f\u5728\u672c\u7cfb\u5217\u6559\u7a0b\u7684\u540e\u9762\uff0c\u6211\u4eec\u8fd8\u4f1a\u7ee7\u7eed\u63a5\u89e6\u5e8f\u5217\u6bd4\u5bf9\u3001\u53d8\u5f02\u68c0\u6d4b\u7b49\u7b49\u64cd\u4f5c\uff0c\u8fd9\u4e9b\u64cd\u4f5c\u4e5f\u90fd\u4f1a\u751f\u6210\u5404\u79cd\u6587\u4ef6\u7c7b\u578b\uff0c\u5c0f\u679c\u5728\u8fd9\u91cc\u5c31\u4e00\u6b21\u6027\u6ee1\u8db3\u5c0f\u4f19\u4f34\u4eec\u7684\u597d\u5947\u5fc3\uff0c\u7ee7\u7eed\u5f80\u4e0b\u5c55\u5f00\u5206\u4eab\u5427~<\/p>\n

    \u4f7f\u7528\u77ed\u5e8f\u5217\u6bd4\u5bf9\u7b97\u6cd5\uff08\u4f8b\u5982Bowtie\u3001BWA\u7b49\uff09\u5c06FASTQ\u6587\u4ef6\u6bd4\u5bf9\u5230\u53c2\u8003\u57fa\u56e0\u7ec4\u6216\u5176\u4ed6\u5e8f\u5217\u5e93\u4e0a\uff0c\u751f\u6210\u7684\u6bd4\u5bf9\u7ed3\u679c\u6587\u4ef6\u901a\u5e38\u662fSAM\uff08Sequence Alignment\/Map\uff09\u6216BAM\uff08Binary Alignment\/Map\uff09\u683c\u5f0f\u3002<\/strong>\u8fd9\u4e9b\u6587\u4ef6\u5305\u542b\u4e86\u6bcf\u4e2a\u6d4b\u5e8f read \u7684\u6bd4\u5bf9\u4f4d\u7f6e\u3001\u5e8f\u5217\u4ee5\u53ca\u5176\u4ed6\u76f8\u5173\u4fe1\u606f\u3002SAM\u6587\u4ef6\u662f\u6587\u672c\u683c\u5f0f\uff0cBAM\u6587\u4ef6\u662f\u5176\u4e8c\u8fdb\u5236\u5f62\u5f0f\u3002SAM\/BAM\u6587\u4ef6\u7684\u4e3b\u8981\u5e94\u7528\u662f\u5b58\u50a8\u548c\u4f20\u8f93\u6d4b\u5e8f\u6570\u636e\u6bd4\u5bf9\u5230\u53c2\u8003\u57fa\u56e0\u7ec4\uff08\u6216\u8f6c\u5f55\u7ec4\uff09\u7684\u7ed3\u679c\u3002\u8fd9\u4e9b\u6587\u4ef6\u5305\u542b\u4e86\u6bcf\u4e2a\u6d4b\u5e8fread\u7684\u6bd4\u5bf9\u4f4d\u7f6e\u3001\u6bd4\u5bf9\u8d28\u91cf\u3001\u5e8f\u5217\u4fe1\u606f\u7b49\u91cd\u8981\u4fe1\u606f\u3002\u5173\u4e8eSAM\/BAM\u6587\u4ef6\u7684\u8be6\u7ec6\u4fe1\u606f\uff0c\u5c0f\u679c\u4f1a\u5728\u540e\u9762\u7684\u6559\u7a0b\u4e2d\u7ed9\u5927\u5bb6\u5a13\u5a13\u9053\u6765\u3002<\/p>\n

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      \n
    1. \u5bf9\u6bd4\u5bf9\u7ed3\u679c\u8fdb\u884c\u53d8\u5f02\u68c0\u6d4b\u65f6\uff0c\u53ef\u4ee5\u5c06SAM\/BAM\u6587\u4ef6\u4e0e\u53c2\u8003\u57fa\u56e0\u7ec4\u8fdb\u884c\u6bd4\u8f83\uff0c\u8bc6\u522b\u51fa\u6d4b\u5e8f\u6837\u672c\u4e2d\u4e0e\u53c2\u8003\u57fa\u56e0\u7ec4\u4e0d\u540c\u7684\u53d8\u5f02\u4f4d\u70b9\uff0c\u5e76\u751f\u6210Variant Call Format\uff08VCF\uff09\u6587\u4ef6\u3002<\/li>\n
    2. \u6839\u636eSAM\/BAM\u6587\u4ef6\u7684\u8986\u76d6\u7a0b\u5ea6\u548c\u7279\u5f81\uff0c\u53ef\u4ee5\u751f\u6210BED\uff08Browser Extensible Data\uff09\u6587\u4ef6\uff0c\u7528\u4e8e\u8868\u793a\u57fa\u56e0\u7ec4\u533a\u57df\u7684\u6ce8\u91ca\u3001\u67d3\u8272\u4f53\u7ed3\u6784\u53d8\u5f02\u7b49\u4fe1\u606f\u3002<\/li>\n
    3. \u5c06\u6bd4\u5bf9\u7ed3\u679c\uff08SAM\/BAM\u6587\u4ef6\uff09\u4e0e\u6ce8\u91ca\u6570\u636e\u5e93\uff08\u5982\u57fa\u56e0\u7ec4\u6ce8\u91ca\u6570\u636e\u5e93\uff09\u7ed3\u5408\uff0c\u751f\u6210GTF\uff08Gene Transfer Format\uff09\u6216GFF\uff08General Feature Format\uff09\u6587\u4ef6\uff0c\u7528\u4e8e\u63cf\u8ff0\u57fa\u56e0\u548c\u8f6c\u5f55\u672c\u7684\u7ed3\u6784\u3001\u5916\u663e\u5b50\u3001\u5185\u542b\u5b50\u7b49\u4fe1\u606f\u3002<\/li>\n
    4. \u4e3a\u4e86\u5feb\u901f\u8bbf\u95ee\u548c\u68c0\u7d22BAM\u6587\u4ef6\u4e2d\u7684\u6bd4\u5bf9\u7ed3\u679c\uff0c\u53ef\u4ee5\u5bf9BAM\u6587\u4ef6\u8fdb\u884c\u7d22\u5f15\u6784\u5efa\u3002\u7d22\u5f15\u6587\u4ef6\u63d0\u4f9b\u4e86\u4e00\u79cd\u5feb\u901f\u67e5\u8be2\u7279\u5b9a\u533a\u57df\u7684\u673a\u5236\uff0c\u907f\u514d\u4e86\u9700\u8981\u9010\u884c\u626b\u63cf\u6574\u4e2aBAM\u6587\u4ef6\u7684\u65f6\u95f4\u6d88\u8017\u3002\u76ee\u524d\u5e38\u89c1\u7684BAM\u7d22\u5f15\u6587\u4ef6\u683c\u5f0f\u6709.BAI\uff08Binary Alignment\/Map Index\uff09\u548c.CSI\uff08Coordinate-sorted Binary Index\uff09\u4e24\u79cd\u683c\u5f0f\u3002<\/li>\n<\/ol>\n

      \u4e3a\u4e86\u65b9\u4fbf\u5927\u5bb6\u8bb0\u5fc6\uff0c\u5c0f\u679c\u81ea\u5236\u4e00\u5f20\u601d\u7ef4\u5bfc\u56fe\uff0c\u6b22\u8fce\u5927\u5bb6\u6536\u85cf\u54e6~<\/p>\n

      <\/p>\n

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