{"id":25295,"date":"2024-01-19T17:06:42","date_gmt":"2024-01-19T09:06:42","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=25295"},"modified":"2024-01-19T17:06:43","modified_gmt":"2024-01-19T09:06:43","slug":"dna%e7%94%9f%e4%bf%a1%e5%88%86%e6%9e%90%ef%bc%88%e5%9b%9b%ef%bc%89%ef%bc%9a%e4%b8%a4%e5%88%86%e9%92%9f%e6%95%99%e4%bd%a0%e5%ae%8c%e6%88%90%e5%ba%8f%e5%88%97%e6%af%94%e5%af%b9","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=25295","title":{"rendered":"DNA\u751f\u4fe1\u5206\u6790\uff08\u56db\uff09\uff1a\u4e24\u5206\u949f\u6559\u4f60\u5b8c\u6210\u5e8f\u5217\u6bd4\u5bf9"},"content":{"rendered":"<p>\u5728\u4e4b\u524d\u7684\u6587\u7ae0\u4e2d\uff0c\u5c0f\u679c\u5411\u5927\u5bb6\u8be6\u7ec6\u5206\u4eab\u4e86\u8d28\u91cf\u8bc4\u4f30\u7684\u64cd\u4f5c\u8fc7\u7a0b\u548c\u7ed3\u679c\u5206\u6790\uff0c\u76f8\u4fe1\u5c0f\u4f19\u4f34\u4eec\u5df2\u7ecf\u719f\u7ec3\u638c\u63e1\uff0c\u8feb\u4e0d\u53ca\u5f85\u5730\u8fdb\u5165\u4e0b\u4e00\u9636\u6bb5\u7684\u5b66\u4e60\u5566\u3002\u8fd9\u4e00\u671f\u5c0f\u679c\u7ed9\u5927\u5bb6\u5e26\u6765\u4e86\u5e8f\u5217\u6bd4\u5bf9\u7684\u64cd\u4f5c\u6b65\u9aa4\uff0c\u597d\u5566\uff0c\u5e9f\u8bdd\u4e0d\u591a\u8bf4\uff0c\u6211\u4eec\u76f4\u63a5\u5f00\u59cb~<\/p>\n<h2>\u5de5\u5177\u4ecb\u7ecd<\/h2>\n<p><strong>\u5e38\u89c1\u7684\u5e8f\u5217\u6bd4\u5bf9\u8f6f\u4ef6\u7c7b\u578b\uff1a<\/strong><\/p>\n<ol>\n<li>\u6570\u636e\u5e93\u641c\u7d22\uff1a<\/li>\n<\/ol>\n<p>BLAST, FASTA, PSI-BLAST\u2026\u2026<\/p>\n<ol>\n<li>\u4e24\u5e8f\u5217\u6bd4\u5bf9\uff1a<\/li>\n<\/ol>\n<p>LASTZ, MUMmer\u2026\u2026<\/p>\n<ol>\n<li>\u591a\u5e8f\u5217\u6bd4\u5bf9\uff1a<\/li>\n<\/ol>\n<p>ClustalW, MUSCLE, T-Coffee<\/p>\n<ol>\n<li>\u57fa\u56e0\u7ec4\u6bd4\u5bf9\uff1a<\/li>\n<\/ol>\n<p>GMAP, BLAT, ACT<\/p>\n<ol>\n<li>\u77ed\u5e8f\u5217\u6bd4\u5bf9\uff1a<\/li>\n<\/ol>\n<p>BWA, SOAP, Bowtie, ELAND, GSNAP, Stampy<\/p>\n<p>\u5c0f\u679c\u8fd9\u6b21\u91c7\u7528\u77ed\u5e8f\u5217\u57fa\u56e0\u7ec4\u6bd4\u5bf9\u5de5\u5177BWA\uff08Burrows-Wheeler Aligner\uff09\u8fdb\u884c\u6bd4\u5bf9\u5206\u6790\u3002\u4ee5\u4e0b\u662fBWA\u8f6f\u4ef6\u4e3b\u9875\uff1a<\/p>\n<p><a href=\"http:\/\/bio-bwa.sourceforge.net\/\">http:\/\/bio-bwa.sourceforge.net\/<\/a><\/p>\n<p>BWA\u5177\u6709\u9ad8\u6548\u5feb\u901f\u3001\u9ad8\u8d28\u91cf\u6bd4\u5bf9\u3001\u652f\u6301\u591a\u79cd\u6bd4\u5bf9\u6a21\u5f0f\u3001\u591a\u7ebf\u7a0b\u652f\u6301\u3001\u7075\u6d3b\u6027\u4e0e\u53ef\u6269\u5c55\u6027\u7b49\u4f18\u70b9\uff0c\u5f88\u9002\u5408\u521d\u5165\u751f\u4fe1\u5927\u95e8\u7684\u5c0f\u4f19\u4f34\u4eec\u4f7f\u7528\u3002<\/p>\n<p>linux\u73af\u5883\u4e0b\uff0c\u8fdb\u884c\u4e0b\u5217\u56db\u6b65\u64cd\u4f5c\u5c31\u53ef\u4ee5\u4f7f\u7528\u5566\uff1a<\/p>\n<p># 1\uff0e\u4e0b\u8f7d\u8f6f\u4ef6\u5b89\u88c5\u5305\uff1a<\/p>\n<p>wget http:\/\/jaist.dl.sourceforge.net\/project\/bio-bwa\/bwa-0.7.12.tar.bz2<\/p>\n<p># 2\uff0e\u89e3\u538b\u7f29\uff1a<br \/>\ntar jxf bwa-0.7.12.tar.bz2<\/p>\n<p># 3\uff0e\u8fdb\u5165\u89e3\u538b\u7f29\u76ee\u5f55\uff1a<br \/>\ncd bwa-0.7.12<\/p>\n<p># 4\uff0e\u7f16\u8bd1\u5b89\u88c5\uff1a<br \/>\nmake<br \/>\n# \u6ce8\u610f\uff1a\u4f7f\u7528\u65f6\u5199\u660e\u5168\u8def\u5f84\uff1a\/home\/bin\/bwa-0.7.12\/bwa\uff0c\u6216\u8005\u8bbe\u7f6e\u73af\u5883\u53d8\u91cf\uff08export PATH=&#8221;\/home\/bin\/bwa-0.7.12:\\$PATH&#8221;\uff0c\u4e5f\u53ef\u5199\u5165\\~\/.bashrc\u5e76source\uff09\u540e\u4ec5\u4f7f\u7528\u7a0b\u5e8f\u540d\u79f0\u3002<\/p>\n<p>BWA\u63d0\u4f9b\u4e86\u4e09\u4e2a\u4e3b\u8981\u7684\u5b50\u547d\u4ee4\uff1abwa index\u3001bwa mem\u548cbwa aln\uff1a<\/p>\n<ol>\n<li>bwa index\uff1a\u7528\u4e8e\u4e3a\u53c2\u8003\u57fa\u56e0\u7ec4\u6784\u5efa\u7d22\u5f15\u6587\u4ef6\uff0c\u4ee5\u4fbf\u4e8e\u540e\u7eed\u7684\u6bd4\u5bf9\u64cd\u4f5c\u3002\u4f7f\u7528\u8be5\u547d\u4ee4\u9700\u8981\u6307\u5b9a\u53c2\u8003\u57fa\u56e0\u7ec4\u7684\u6587\u4ef6\uff0c\u5e76\u751f\u6210\u4e00\u7cfb\u5217\u7d22\u5f15\u6587\u4ef6\uff08\u5982.bwt\u3001.sa\u7b49\uff09\uff0c\u8fd9\u4e9b\u7d22\u5f15\u6587\u4ef6\u5c06\u5728\u540e\u7eed\u7684\u6bd4\u5bf9\u64cd\u4f5c\u4e2d\u88ab\u4f7f\u7528\u3002<\/li>\n<li>bwa mem\uff1a\u8fd9\u662fBWA\u4e2d\u6700\u5e38\u7528\u7684\u5b50\u547d\u4ee4\uff0c\u9002\u7528\u4e8e\u5904\u7406 Illumina \u6d4b\u5e8f\u6570\u636e\u3002\u5b83\u53ef\u4ee5\u5c06\u6d4b\u5e8f\u6570\u636e\uff08fastq\u683c\u5f0f\uff09\u4e0e\u53c2\u8003\u57fa\u56e0\u7ec4\u8fdb\u884c\u6bd4\u5bf9\u3002\u4f7f\u7528\u8be5\u547d\u4ee4\u9700\u8981\u6307\u5b9a\u53c2\u8003\u57fa\u56e0\u7ec4\u7684\u7d22\u5f15\u6587\u4ef6\u3001\u6d4b\u5e8f\u6570\u636e\u6587\u4ef6\uff0c\u5e76\u53ef\u4ee5\u9009\u62e9\u6027\u5730\u6307\u5b9a\u5176\u4ed6\u53c2\u6570\uff0c\u5982\u7ebf\u7a0b\u6570\u3001\u6bd4\u5bf9\u5f97\u5206\u7b49\u3002\u6bd4\u5bf9\u7ed3\u679c\u5c06\u8f93\u51fa\u4e3aSAM\u683c\u5f0f\u6587\u4ef6\u3002<\/li>\n<li>bwa aln\uff1a\u8be5\u547d\u4ee4\u9002\u7528\u4e8e\u5904\u7406\u8f83\u77ed\u7684\u6d4b\u5e8f\u7247\u6bb5\uff0c\u5982 Illumina \u6d4b\u5e8f\u4e2d\u7684\u77ed\u8bfb\u957f\u3002\u5b83\u5c06\u6d4b\u5e8f\u6570\u636e\u4e0e\u53c2\u8003\u57fa\u56e0\u7ec4\u8fdb\u884c\u6bd4\u5bf9\uff0c\u5e76\u751f\u6210\u6bd4\u5bf9\u4f4d\u7f6e\u7684SA\uff08Suffix Array\uff09\u5750\u6807\u3002\u9700\u8981\u6307\u5b9a\u53c2\u8003\u57fa\u56e0\u7ec4\u7684\u7d22\u5f15\u6587\u4ef6\u3001\u6d4b\u5e8f\u6570\u636e\u6587\u4ef6\uff0c\u5e76\u53ef\u4ee5\u9009\u62e9\u6027\u5730\u6307\u5b9a\u5176\u4ed6\u53c2\u6570\uff0c\u5982mismatch\u60e9\u7f5a\u3001gap\u5f00\u9500\u7b49\u3002<\/li>\n<\/ol>\n<p>\u9664\u4e86\u4e0a\u8ff0\u6838\u5fc3\u547d\u4ee4\u5916\uff0cBWA\u8fd8\u63d0\u4f9b\u4e86\u5176\u4ed6\u4e00\u4e9b\u8f85\u52a9\u547d\u4ee4\u548c\u5de5\u5177\uff0c\u7528\u4e8e\u7d22\u5f15\u7684\u66f4\u65b0\u3001\u683c\u5f0f\u8f6c\u6362\u548c\u7edf\u8ba1\u5206\u6790\u7b49\u3002\u4f8b\u5982\uff0cbwa samse\u548cbwa sampe\u7528\u4e8e\u5904\u7406\u77ed\u8bfb\u957f\u6d4b\u5e8f\u6570\u636e\u7684\u6bd4\u5bf9\u7ed3\u679c\uff1bbwa fastq2fasta\u7528\u4e8e\u5c06fastq\u683c\u5f0f\u6587\u4ef6\u8f6c\u6362\u4e3afasta\u683c\u5f0f\u6587\u4ef6\uff1bbwa stats\u7528\u4e8e\u751f\u6210\u5173\u4e8e\u6bd4\u5bf9\u6548\u679c\u7684\u7edf\u8ba1\u4fe1\u606f\u7b49\u3002\u60f3\u8981\u4e86\u89e3\u66f4\u591a\u7684\u5c0f\u4f19\u4f34\u4eec\u53ef\u4ee5\u67e5\u9605\u5b98\u65b9\u6587\u6863\u54e6~<\/p>\n<p>\u53e6\u5916\u8fd9\u6b21\u8fd8\u9700\u8981\u7528\u5230Samtools\uff0cSamtools \u662f\u4e00\u4e2a\u7528\u4e8e\u5904\u7406SAM\u548cBAM\u683c\u5f0f\u6587\u4ef6\u7684\u5de5\u5177\u96c6\u3002\u5b83\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u547d\u4ee4\u884c\u5de5\u5177\uff0c\u7528\u4e8e\u5bf9\u6bd4\u5bf9\u7ed3\u679c\u8fdb\u884c\u64cd\u4f5c\u3001\u8f6c\u6362\u548c\u5206\u6790\u3002<\/p>\n<p>\u4ee5\u4e0b\u662f\u4e00\u4e9b\u5e38\u7528\u7684Samtools\u547d\u4ee4\uff1a<\/p>\n<ol>\n<li>samtools view: \u7528\u4e8e\u67e5\u770bSAM\/BAM\u6587\u4ef6\u7684\u5185\u5bb9\u3002\u53ef\u4ee5\u9009\u62e9\u6027\u5730\u8f93\u51fa\u6307\u5b9a\u533a\u57df\u7684\u6bd4\u5bf9\u4fe1\u606f\u6216\u8fc7\u6ee4\u7279\u5b9a\u6761\u4ef6\u4e0b\u7684\u6bd4\u5bf9\u7ed3\u679c\u3002<\/li>\n<li>samtools sort: \u5bf9BAM\u6587\u4ef6\u4e2d\u7684\u6bd4\u5bf9\u8bb0\u5f55\u6309\u7167\u67d3\u8272\u4f53\u4f4d\u7f6e\u8fdb\u884c\u6392\u5e8f\uff0c\u751f\u6210\u6392\u5e8f\u540e\u7684BAM\u6587\u4ef6\u3002\u8fd9\u6709\u52a9\u4e8e\u540e\u7eed\u7684\u7d22\u5f15\u548c\u6570\u636e\u5206\u6790\u3002<\/li>\n<li>samtools index: \u4e3aBAM\u6587\u4ef6\u521b\u5efa\u7d22\u5f15\u6587\u4ef6\uff0c\u4ee5\u4fbf\u5728\u540e\u7eed\u7684\u67e5\u8be2\u548c\u8bbf\u95ee\u4e2d\u5feb\u901f\u5b9a\u4f4d\u6bd4\u5bf9\u8bb0\u5f55\u3002<\/li>\n<li>samtools merge: \u5c06\u591a\u4e2aBAM\u6587\u4ef6\u5408\u5e76\u6210\u4e00\u4e2a\u5355\u4e00\u7684BAM\u6587\u4ef6\uff0c\u65b9\u4fbf\u540e\u7eed\u7684\u6bd4\u5bf9\u7ed3\u679c\u6574\u5408\u548c\u5206\u6790\u3002<\/li>\n<li>samtools flagstat: \u7edf\u8ba1BAM\u6587\u4ef6\u4e2d\u6bd4\u5bf9\u8bb0\u5f55\u7684\u5404\u79cd\u6807\u5fd7\uff08flag\uff09\u7684\u7edf\u8ba1\u4fe1\u606f\uff0c\u4f8b\u5982\u603b\u6bd4\u5bf9\u6570\u91cf\u3001\u500d\u6570\u6bd4\u5bf9\u6570\u91cf\u3001\u672a\u6bd4\u5bf9\u6570\u91cf\u7b49\u3002<\/li>\n<li>samtools mpileup: \u5728\u7ed9\u5b9a\u7684\u4f4d\u7f6e\u4e0a\u5bf9\u6bd4\u5bf9\u8bb0\u5f55\u8fdb\u884c\u5806\u53e0\uff0c\u751f\u6210\u5806\u53e0\u4fe1\u606f\uff0c\u7528\u4e8e\u53d8\u5f02\u68c0\u6d4b\u548c\u6df1\u5ea6\u5206\u6790\u3002<\/li>\n<li>samtools faidx: \u4e3aFASTA\u683c\u5f0f\u7684\u53c2\u8003\u57fa\u56e0\u7ec4\u6587\u4ef6\u521b\u5efa\u7d22\u5f15\u6587\u4ef6\uff0c\u4ee5\u4fbf\u5728\u540e\u7eed\u7684\u67e5\u8be2\u548c\u8bbf\u95ee\u4e2d\u5feb\u901f\u63d0\u53d6\u5e8f\u5217\u3002<\/li>\n<li>samtools calmd: \u7528\u4e8e\u7ed9BAM\u6587\u4ef6\u6dfb\u52a0\u6216\u66f4\u65b0\u6bd4\u5bf9\u8bb0\u5f55\u7684MD\u6807\u7b7e\uff0c\u8fd9\u4e2a\u6807\u7b7e\u8868\u793a\u4e86\u6bd4\u5bf9\u65f6\u53ef\u80fd\u7684\u78b1\u57fa\u5dee\u5f02\u3002<\/li>\n<\/ol>\n<p>\u4ee5\u4e0a\u53ea\u662fSamtools\u547d\u4ee4\u96c6\u4e2d\u7684\u4e00\u90e8\u5206\uff0cSamtools\u8fd8\u63d0\u4f9b\u4e86\u5176\u4ed6\u529f\u80fd\u5f3a\u5927\u7684\u547d\u4ee4\u548c\u9009\u9879\uff0c\u7528\u4e8e\u5904\u7406\u548c\u5206\u6790\u6bd4\u5bf9\u6570\u636e\u3002\u5b83\u5728\u751f\u7269\u4fe1\u606f\u5b66\u9886\u57df\u5e7f\u6cdb\u5e94\u7528\u4e8e\u6d4b\u5e8f\u6570\u636e\u7684\u5904\u7406\u3001\u53d8\u5f02\u68c0\u6d4b\u3001\u6bd4\u5bf9\u8bc4\u4f30\u4ee5\u53ca\u5176\u4ed6\u76f8\u5173\u4efb\u52a1\u3002<\/p>\n<h2>\u64cd\u4f5c\u6b65\u9aa4<\/h2>\n<p>\u4e66\u63a5\u4e0a\u56de\uff0c\u6211\u4eec\u76f4\u63a5\u7528\u524d\u9762\u5df2\u7ecf\u8bbe\u7f6e\u597d\u8def\u5f84\u7684\u6570\u636e\u7ee7\u7eed\u5f00\u59cb\uff0c\u5c0f\u679c\u5148\u628a\u5e8f\u5217\u6bd4\u5bf9\u8fc7\u7a0b\u7684\u5168\u4ee3\u7801\u4e00\u6b21\u6027\u653e\u5728\u8fd9\u91cc\uff0c\u65b9\u4fbf\u5c0f\u4f19\u4f34\u4eec\u4f7f\u7528~\uff1a<\/p>\n<p># \u4e3a\u53c2\u8003\u5e8f\u5217\u5efa\u7acb\u7d22\u5f15<br \/>\nbwa index .\/00_ref\/MGI358.SNP.fa<br \/>\n# \u5c06\u6d4b\u5e8freads\u6bd4\u5bf9\u5230\u53c2\u8003\u5e8f\u5217<br \/>\nbwa mem -M -Y -t 1 .\/00_ref\/MGI358.SNP.fa .\/01_clean\/test_clean.fq.gz &gt; .\/02_align\/test.clean.sam<br \/>\n# \u4e3a\u8282\u7ea6\u5b58\u50a8\uff0c\u5c06sam\u8f6c\u6362\u4e3a\u4e8c\u8fdb\u5236\u6587\u4ef6bam\uff0c\u5e76\u5bf9\u6bd4\u5bf9\u7ed3\u679c\u8fdb\u884c\u6392\u5e8f<br \/>\nsamtools sort .\/02_align\/test.clean.sam -o .\/02_align\/test.clean.sort.bam<br \/>\n# \u4e3a\u6392\u5e8f\u540e\u7684bam\u6587\u4ef6\u521b\u5efa\u7d22\u5f15<br \/>\nsamtools index .\/02_align\/test.clean.sort.bam<br \/>\n# \u4f7f\u7528samtools\u7b5b\u9009\u51fa\u975e\u91cd\u590d\u6bd4\u5bf9\u7684reads\uff0c\u8f93\u51fa\u4e3abam\u6587\u4ef6<br \/>\nsamtools view -F 256 -hb .\/02_align\/test.clean.sort.bam &gt; .\/02_align\/test.clean.sort.uniq.bam<br \/>\n# \u7edf\u8ba1\u8986\u76d6\u6df1\u5ea6<br \/>\nbamdst -p .\/00_ref\/target.358.SE50.subSNP.bed -o .\/02_align .\/02_align\/test.clean.sort.uniq.bam<\/p>\n<p>\u8fd9\u4e9b\u5c31\u662f\u64cd\u4f5c\u9700\u8981\u7684\u5168\u90e8\u4ee3\u7801\u5566\uff0c\u662f\u4e0d\u662f\u5f88\u7b80\u5355\u5462~\u5982\u679c\u5c0f\u4f19\u4f34\u4eec\u5e73\u65f6\u5728\u751f\u4fe1\u5206\u6790\u7684\u64cd\u4f5c\u8fc7\u7a0b\u4e2d\u9047\u5230\u56f0\u96be\uff0c\u6b22\u8fce\u5927\u5bb6\u4f7f\u7528\u5c0f\u679c\u5f00\u53d1\u7684\u751f\u4fe1\u5de5\u5177\u5e73\u53f0<a href=\"http:\/\/www.biocloudservice.com\/home.html\">http:\/\/www.biocloudservice.com\/home.html<\/a>\uff0c\u66f4\u52a0\u65b9\u4fbf\u5feb\u6377\u54e6~<\/p>\n<p>\u4e0b\u9762\uff0c\u5c0f\u679c\u5c31\u7ed9\u5927\u5bb6\u8be6\u7ec6\u5730\u5bf9\u7ec6\u8282\u95ee\u9898\u8fdb\u884c\u4ecb\u7ecd\uff0c\u60f3\u63d0\u5347\u81ea\u5df1\u7684\u5c0f\u4f19\u4f34\u4eec\u4e0d\u8981\u8d70\u5f00\u54e6~<\/p>\n<h2>\u5206\u6b65\u8be6\u7ec6\u4ecb\u7ecd<\/h2>\n<p># \u4e3a\u53c2\u8003\u5e8f\u5217\u5efa\u7acb\u7d22\u5f15<br \/>\nbwa index .\/00_ref\/MGI358.SNP.fa<\/p>\n<p>\u7d22\u5f15\u7684\u76ee\u7684\u662f\u4e3a\u4e86\u52a0\u5feb\u540e\u7eed\u7684\u6bd4\u5bf9\u8fc7\u7a0b\u3002BWA\u5728\u6bd4\u5bf9\u65f6\u9700\u8981\u5148\u52a0\u8f7d\u7d22\u5f15\uff0c\u4ee5\u83b7\u5f97\u66f4\u9ad8\u7684\u6bd4\u5bf9\u6548\u7387\u548c\u51c6\u786e\u6027\u3002<\/p>\n<p>\u6211\u4eec\u628a\u7d22\u5f15\u5b8c\u6210\u540e\u7684\u6587\u4ef6\u5939\u4e0b\u8f7d\u5230\u672c\u5730\u4fbf\u4e8e\u89c2\u770b\uff0c\u4f1a\u53d1\u73b0\u7d22\u5f15\u64cd\u4f5c\u751f\u6210\u4e86\u591a\u4e2a\u6587\u4ef6\uff0c\u5305\u62ec.bwt\u3001 .fai\u3001.pac\u3001.ann\u3001.amb\u548c.sa\u7b49\u6587\u4ef6\uff0c\u5b83\u4eec\u4f5c\u4e3aBWA\u6bd4\u5bf9\u8fc7\u7a0b\u7684\u8f93\u5165\u4e4b\u4e00\u3002\u8fd9\u4e9b\u7d22\u5f15\u6587\u4ef6\u80fd\u591f\u5e2e\u52a9BWA\u5feb\u901f\u5730\u4ece\u53c2\u8003\u57fa\u56e0\u7ec4\u4e2d\u641c\u7d22\u5339\u914d\u7684\u7247\u6bb5\uff0c\u5e76\u8fdb\u884c\u6bd4\u5bf9\uff0c\u5177\u4f53\u4ecb\u7ecd\u5982\u4e0b\uff1a<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"346\" class=\"wp-image-25296\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-1.png?resize=640%2C346\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-1.png?w=1392 1392w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-1.png?resize=300%2C162 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-1.png?resize=1024%2C553 1024w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-1.png?resize=768%2C415 768w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-1.png?resize=600%2C324 600w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-1.png?w=1280 1280w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/><\/p>\n<ol>\n<li>.bwt \u6587\u4ef6\uff1aBurrows-Wheeler\u8f6c\u6362\u540e\u7684\u7d22\u5f15\u6587\u4ef6\uff0c\u5305\u542b\u4e86\u5bf9\u53c2\u8003\u57fa\u56e0\u7ec4\u7684\u538b\u7f29\u548c\u8f6c\u6362\u4fe1\u606f\uff0c\u7528\u4e8e\u5feb\u901f\u641c\u7d22\u5339\u914d\u7684\u7247\u6bb5\u3002<\/li>\n<li>.pac \u6587\u4ef6\uff1a\u53e6\u4e00\u79cd\u538b\u7f29\u683c\u5f0f\u7684\u7d22\u5f15\u6587\u4ef6\uff0c\u8bb0\u5f55\u4e86\u53c2\u8003\u57fa\u56e0\u7ec4\u7684\u78b1\u57fa\u4fe1\u606f\uff0c\u63d0\u4f9b\u7ed9BWA\u8fdb\u884c\u6bd4\u5bf9\u3002<\/li>\n<li>.ann \u6587\u4ef6\uff1a\u6ce8\u91ca\u6587\u4ef6\uff0c\u8bb0\u5f55\u4e86\u53c2\u8003\u57fa\u56e0\u7ec4\u4e2d\u7684\u6ce8\u91ca\u4fe1\u606f\uff0c\u4f8b\u5982\u533a\u57df\u540d\u79f0\u3001\u67d3\u8272\u4f53\u4f4d\u7f6e\u7b49\u3002<\/li>\n<li>.amb \u6587\u4ef6\uff1a\u5224\u65ad\u78b1\u57fa\u6a21\u68f1\u4e24\u53ef\uff08ambiguous\uff09\u7684\u4f4d\u70b9\uff0c\u5373\u591a\u4e2a\u78b1\u57fa\u53ef\u80fd\u6027\u5e76\u5b58\u7684\u4f4d\u7f6e\u3002<\/li>\n<li>.sa \u6587\u4ef6\uff1a\u540e\u7f00\u6570\u7ec4\u7d22\u5f15\u6587\u4ef6\uff0c\u5305\u542b\u4e86\u540e\u7f00\u6570\u7ec4\u7684\u4fe1\u606f\uff0c\u7528\u4e8e\u641c\u7d22\u5339\u914d\u7684\u540e\u7f00\u3002<\/li>\n<li>.fai \u6587\u4ef6\uff1a\u8bb0\u5f55\u4e86\u6bcf\u6761\u67d3\u8272\u4f53\/\u5e8f\u5217\u7684\u540d\u79f0\u3001\u957f\u5ea6\u4ee5\u53ca\u5728\u7d22\u5f15\u8fc7\u7a0b\u4e2d\u7684\u987a\u5e8f\uff0c\u67d0\u4e9b\u53d8\u79cd\u8c03\u7528\u7a0b\u5e8f\u9700\u8981\u8bfb\u53d6 .fai \u6587\u4ef6\u4ee5\u786e\u5b9a\u67d3\u8272\u4f53\u7684\u4f4d\u7f6e\u548c\u5e8f\u5217\u957f\u5ea6\u3002<\/li>\n<\/ol>\n<p># \u5c06\u6d4b\u5e8freads\u6bd4\u5bf9\u5230\u53c2\u8003\u5e8f\u5217<br \/>\nbwa mem -M -Y -t 1 .\/00_ref\/MGI358.SNP.fa .\/01_clean\/test_clean.fq.gz &gt; .\/02_align\/test.clean.sam<br \/>\n# \u7528less\u547d\u4ee4\u67e5\u770b\u5b58\u50a8\u6bd4\u5bf9\u4fe1\u606f\u7684\u5e8f\u5217\u6587\u4ef6sam<br \/>\nless .\/02_align\/test.clean.sam<\/p>\n<p>\u8fd9\u6bb5\u4ee3\u7801\u770b\u8d77\u6765\u6709\u4e00\u70b9\u957f\uff0c\u5c0f\u679c\u5728\u8fd9\u91cc\u4e3a\u5927\u5bb6\u62c6\u5f00\u8be6\u7ec6\u4ecb\u7ecd\uff1a<\/p>\n<ul>\n<li>bwa mem: \u8868\u793a\u8c03\u7528BWA\u7a0b\u5e8f\u7684mem\u5b50\u547d\u4ee4\uff0c\u7528\u4e8e\u6267\u884c\u6bd4\u5bf9\u64cd\u4f5c\u3002<\/li>\n<li>-M: \u8fd9\u4e2a\u9009\u9879\u544a\u8bc9BWA\u8981\u751f\u6210\u4e00\u4e2a\u5305\u542bMate-SW\u4fe1\u606f\u7684SAM\u6587\u4ef6\u3002Mate-SW\u4fe1\u606f\u4e3b\u8981\u7528\u4e8e\u5355\u7aef\u6570\u636e\u4e0e\u914d\u5bf9\u7684\u6bd4\u5bf9\u7ed3\u679c\u4e00\u8d77\u4f7f\u7528\u3002<\/li>\n<li>-Y: \u8fd9\u4e2a\u9009\u9879\u544a\u8bc9BWA\u751f\u6210\u4e00\u4e2a\u538b\u7f29\u7684\u8f93\u51fa\u6587\u4ef6\uff08\u5373SAM\u6587\u4ef6\uff09\u3002\u538b\u7f29\u53ef\u4ee5\u51cf\u5c11\u5b58\u50a8\u7a7a\u95f4\u7684\u4f7f\u7528\u3002<\/li>\n<li>-t 1: \u8fd9\u4e2a\u9009\u9879\u6307\u5b9a\u7ebf\u7a0b\u6570\u4e3a1\uff0c\u8868\u793a\u4f7f\u7528\u5355\u7ebf\u7a0b\u8fd0\u884cBWA\u3002\u5982\u679c\u4f60\u6709\u66f4\u591a\u7684CPU\u6838\u5fc3\u53ef\u7528\uff0c\u53ef\u4ee5\u8c03\u6574\u8be5\u503c\u4ee5\u52a0\u5feb\u901f\u5ea6\u3002<\/li>\n<li>.\/00_ref\/MGI358.SNP.fa: \u8fd9\u662f\u53c2\u8003\u57fa\u56e0\u7ec4\u7684\u6587\u4ef6\u8def\u5f84\u548c\u6587\u4ef6\u540d\u3002<\/li>\n<li>.\/01_clean\/test_clean.fq.gz: \u8fd9\u662f\u7ecf\u8fc7\u6e05\u6d17\u540e\u7684\u6d4b\u5e8f\u6570\u636e\uff08fastq\u683c\u5f0f\uff09\u7684\u6587\u4ef6\u8def\u5f84\u548c\u6587\u4ef6\u540d\u3002<\/li>\n<li>&gt; .\/02_align\/test.clean.sam: \u8fd9\u90e8\u5206\u4ee3\u7801\u5c06\u6bd4\u5bf9\u7ed3\u679c\u8f93\u51fa\u5230\u4e00\u4e2a\u540d\u4e3a test.clean.sam \u7684SAM\u6587\u4ef6\u3002&gt; \u7b26\u53f7\u5c06\u6807\u51c6\u8f93\u51fa\u91cd\u5b9a\u5411\u5230\u6307\u5b9a\u7684\u6587\u4ef6\u8def\u5f84\u3002<\/li>\n<\/ul>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"350\" class=\"wp-image-25297\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-2.png?resize=640%2C350\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-2.png?w=1388 1388w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-2.png?resize=300%2C164 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-2.png?resize=1024%2C559 1024w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-2.png?resize=768%2C419 768w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-2.png?resize=600%2C328 600w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-2.png?w=1280 1280w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/><\/p>\n<p>\u901a\u8fc7\u6267\u884c\u8fd9\u6bb5\u4ee3\u7801\uff0cBWA\u4f1a\u4f7f\u7528\u5355\u7ebf\u7a0b\u5c06\u6e05\u6d17\u540e\u7684\u6d4b\u5e8f\u6570\u636e\u4e0e\u53c2\u8003\u57fa\u56e0\u7ec4\u8fdb\u884c\u6bd4\u5bf9\uff0c\u751f\u6210\u4e00\u4e2aSAM\u683c\u5f0f\u7684\u6bd4\u5bf9\u7ed3\u679c\u6587\u4ef6\u3002SAM\u6587\u4ef6\u4e2d\u5305\u542b\u4e86\u6bcf\u4e2a\u6d4b\u5e8f\u8bfb\u53d6\u7684\u6bd4\u5bf9\u4f4d\u7f6e\u3001\u6bd4\u5bf9\u8d28\u91cf\u7b49\u4fe1\u606f\uff0c\u53ef\u4ee5\u7528\u4e8e\u540e\u7eed\u7684\u53d8\u5f02\u68c0\u6d4b\u3001\u6bd4\u5bf9\u7edf\u8ba1\u548c\u5176\u4ed6\u5206\u6790\u64cd\u4f5c\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"342\" class=\"wp-image-25298\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-3.png?resize=640%2C342\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-3.png?w=1920 1920w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-3.png?resize=300%2C160 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-3.png?resize=1024%2C548 1024w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-3.png?resize=768%2C411 768w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-3.png?resize=1536%2C822 1536w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-3.png?resize=600%2C321 600w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-3.png?w=1280 1280w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/><\/p>\n<p>\u8fd9\u6bb5SAM\u6587\u4ef6\u5c55\u793a\u4e86\u6d4b\u5e8f\u6570\u636e\u4e2dSNP\u7684rs\u7f16\u53f7\u4e0e\u4f4d\u7f6e\u7684\u5bf9\u5e94\u5173\u7cfb\u3002<\/p>\n<p># \u4e3a\u8282\u7ea6\u5b58\u50a8\uff0c\u5c06sam\u8f6c\u6362\u4e3a\u4e8c\u8fdb\u5236\u6587\u4ef6bam\uff0c\u5e76\u5bf9\u6bd4\u5bf9\u7ed3\u679c\u8fdb\u884c\u6392\u5e8f<br \/>\nsamtools sort .\/02_align\/test.clean.sam -o .\/02_align\/test.clean.sort.bam<br \/>\n# \u4e3a\u6392\u5e8f\u540e\u7684bam\u6587\u4ef6\u521b\u5efa\u7d22\u5f15<br \/>\nsamtools index .\/02_align\/test.clean.sort.bam<br \/>\n# \u4f7f\u7528samtools\u7b5b\u9009\u51fa\u975e\u91cd\u590d\u6bd4\u5bf9\u7684reads\uff0c\u8f93\u51fa\u4e3abam\u6587\u4ef6<br \/>\nsamtools view -F 256 -hb .\/02_align\/test.clean.sort.bam &gt; .\/02_align\/test.clean.sort.uniq.bam<br \/>\n# \u67e5\u770bbam\u6587\u4ef6\u7684\u5185\u5bb9<br \/>\nsamtools view -S .\/02_align\/test.clean.sort.bam | less -S<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"342\" class=\"wp-image-25299\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-4.png?resize=640%2C342\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-4.png?w=1920 1920w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-4.png?resize=300%2C160 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-4.png?resize=1024%2C548 1024w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-4.png?resize=768%2C411 768w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-4.png?resize=1536%2C822 1536w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-4.png?resize=600%2C321 600w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-4.png?w=1280 1280w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/><\/p>\n<p>\u8fd9\u6bb5BAM\u6587\u4ef6\u4e3b\u8981\u5c55\u793a\u4e86SNP\u4f4d\u70b9rs1490413\u7684\u90e8\u5206\u6d4b\u5e8f\u6570\u636e\u7ed3\u679c\u30021001\u4e3a\u67d3\u8272\u4f53\u4f4d\u7f6e\uff0c60\u4e3a\u6620\u5c04\u8d28\u91cf\uff0c\u6700\u53f3\u9762\u7684\u5e8f\u5217\u4e3a\u78b1\u57fa\u5e8f\u5217\u3002<\/p>\n<p># \u7edf\u8ba1\u8986\u76d6\u6df1\u5ea6<br \/>\nbamdst -p .\/00_ref\/target.358.SE50.subSNP.bed -o .\/02_align .\/02_align\/test.clean.sort.uniq.bam<br \/>\n# \u67e5\u770b\u8986\u76d6\u6df1\u5ea6\u7edf\u8ba1\u60c5\u51b5<br \/>\nless .\/02_align\/depth.tsv.gz<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"344\" class=\"wp-image-25300\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-5.png?resize=640%2C344\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-5.png?w=1920 1920w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-5.png?resize=300%2C161 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-5.png?resize=1024%2C550 1024w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-5.png?resize=768%2C412 768w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-5.png?resize=1536%2C825 1536w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-5.png?resize=600%2C322 600w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/01\/word-image-25295-5.png?w=1280 1280w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/><\/p>\n<p>\u8fd9\u6bb5\u6587\u4ef6\u5c55\u793aSNP\u4f4d\u70b9rs1490413\u7684\u8986\u76d6\u6df1\u5ea6\u7edf\u8ba1\u7ed3\u679c<\/p>\n<p>\u8bf4\u660e\uff1a<\/p>\n<p>Pos: \u67d3\u8272\u4f53\u4e0a\u4f4d\u7f6e\u4fe1\u606f<\/p>\n<p>Raw Depth: \u539f\u59cb\u6df1\u5ea6\uff0c\u5373\u6d4b\u5e8f\u6570\u636e\u4e2d\u7279\u5b9a\u4f4d\u7f6e\u7684\u8bfb\u53d6\u6570\u91cf<\/p>\n<p>Rmdup Depth: \u53bb\u9664\u5197\u4f59\u540e\u7684\u6df1\u5ea6\uff0c\u6392\u9664\u91cd\u590d\u7684\u8bfb\u53d6\u540e\u7279\u5b9a\u4f4d\u7f6e\u7684\u8bfb\u53d6\u6570\u91cf<\/p>\n<p>Cover Depth: \u8986\u76d6\u6df1\u5ea6\uff0c\u8868\u793a\u7279\u5b9a\u4f4d\u7f6e\u88ab\u8bfb\u53d6\u8986\u76d6\u7684\u6b21\u6570<\/p>\n<p>\u5728\u6700\u540e\uff0c\u5c0f\u679c\u7ed9\u5c0f\u4f19\u4f34\u4eec\u603b\u7ed3\u4e00\u4e0b\u5b9e\u9a8c\u7684\u8fc7\u7a0b\uff1a\u9996\u5148\u4f7f\u7528BWA\u8f6f\u4ef6\u5bf9\u53c2\u8003\u5e8f\u5217\u5efa\u7acb\u7d22\u5f15\uff0c\u7136\u540e\u5c06\u6d4b\u5e8freads\u4e0e\u53c2\u8003\u5e8f\u5217\u8fdb\u884c\u6bd4\u5bf9\u3002\u6bd4\u5bf9\u7ed3\u679c\u4ee5SAM\u683c\u5f0f\u5b58\u50a8\uff0c\u53ef\u4ee5\u901a\u8fc7Samtools\u8fdb\u884c\u67e5\u770b\u548c\u5904\u7406\u3002\u4e3a\u4e86\u8282\u7ea6\u5b58\u50a8\u7a7a\u95f4\uff0c\u5c06SAM\u6587\u4ef6\u8f6c\u6362\u4e3aBAM\u6587\u4ef6\uff0c\u5e76\u5bf9\u6bd4\u5bf9\u7ed3\u679c\u8fdb\u884c\u6392\u5e8f\u548c\u7d22\u5f15\u3002\u8fdb\u4e00\u6b65\uff0c\u7edf\u8ba1\u4e86\u8986\u76d6\u6df1\u5ea6\uff0c\u4ee5\u8bc4\u4f30\u6d4b\u5e8f\u6570\u636e\u7684\u8d28\u91cf\u548c\u8986\u76d6\u5ea6\u3002 \u6700\u7ec8\uff0c\u5b9e\u9a8c\u7ed3\u679c\u5305\u62ecSAM\u6587\u4ef6\uff0cBAM\u6587\u4ef6\uff0c\u8986\u76d6\u6df1\u5ea6\u7edf\u8ba1\u6570\u636e\uff0c\u4ee5\u53ca\u6bd4\u5bf9\u7ed3\u679c\u7684\u53ef\u89c6\u5316\u5c55\u793a\u548c\u611f\u5174\u8da3\u7684\u53d8\u5f02\u4f4d\u70b9\u7684\u67e5\u770b\u3002<\/p>\n<p>\u5982\u679c\u5c0f\u4f19\u4f34\u4eec\u5e73\u65f6\u5728\u751f\u4fe1\u5206\u6790\u7684\u64cd\u4f5c\u8fc7\u7a0b\u4e2d\u9047\u5230\u56f0\u96be\uff0c\u6b22\u8fce\u5927\u5bb6\u4f7f\u7528\u5c0f\u679c\u5f00\u53d1\u7684\u751f\u4fe1\u5de5\u5177\u5e73\u53f0<a href=\"http:\/\/www.biocloudservice.com\/home.html\">http:\/\/www.biocloudservice.com\/home.html<\/a>\uff0c\u5927\u5bb6\u5728\u65b0\u63a5\u89e6\u4e00\u4e2a\u77e5\u8bc6\u7684\u65f6\u5019\uff0c\u4e0e\u5176\u5148\u82b1\u8d39\u5927\u91cf\u65f6\u95f4\u6b7b\u5543\u77e5\u8bc6\u70b9\uff0c\u4e0d\u5982\u5148\u5229\u7528\u597d\u5de5\u5177\u5148\u81ea\u5df1\u4e0a\u624b\u63a5\u89e6\u6d41\u7a0b\uff0c\u5728\u8dd1\u5b8c\u4e00\u904d\u5168\u6d41\u7a0b\u540e\u518d\u8fd4\u56de\u53bb\u7406\u89e3\u77e5\u8bc6\u70b9\uff0c\u76f8\u4fe1\u53ef\u4ee5\u66f4\u597d\u66f4\u5feb\u5730\u7406\u89e3\uff0c\u8fbe\u5230\u4e8b\u534a\u529f\u500d\u7684\u6548\u679c\uff01\u6bd4\u5982\u8fd9\u4e00\u6b21\u5c0f\u679c\u5c31\u5148\u628a\u5e8f\u5217\u6bd4\u5bf9\u7684\u64cd\u4f5c\u8fc7\u7a0b\u7ed9\u5927\u5bb6\u5206\u4eab\u51fa\u6765\uff0c\u5e2e\u52a9\u5927\u5bb6\u5b9e\u9645\u64cd\u4f5c\u4e00\u904d\u540e\u53c8\u8be6\u7ec6\u5730\u8fdb\u884c\u4e86\u5206\u6b65\u4ecb\u7ecd\u3002\u4e0b\u4e00\u6b21\u5c0f\u679c\u4f1a\u7ed9\u5927\u5bb6\u8be6\u7ec6\u5730\u4ecb\u7ecd\u5e8f\u5217\u6bd4\u5bf9\u7684\u539f\u7406\uff0c\u60f3\u8981\u8fdb\u9636\u63d0\u5347\u3001\u6210\u957f\u4e3a\u751f\u4fe1\u5927\u5e08\u7684\u5c0f\u4f19\u4f34\u4eec\u5343\u4e07\u4e0d\u8981\u9519\u8fc7\u54e6~<\/p>\n<p>\u53e6\u5916\uff0c\u4e3a\u4e86\u66f4\u597d\u5730\u7406\u89e3\u6d4b\u5e8f\u7247\u6bb5\u5728\u53c2\u8003\u5e8f\u5217\u4e0a\u7684\u4f4d\u7f6e\u548c\u53d8\u5f02\u60c5\u51b5\uff0c\u53ef\u4ee5\u4f7f\u7528IGV\u8f6f\u4ef6\u8fdb\u884c\u6bd4\u5bf9\u7ed3\u679c\u7684\u53ef\u89c6\u5316\uff0c\u60f3\u8981\u5b66\u4e60IGV\u7684\u5c0f\u4f19\u4f34\u4eec\u8bb0\u5f97\u6301\u7eed\u5173\u6ce8\u5c0f\u679c\uff0c\u5c0f\u679c\u4e5f\u4f1a\u5728\u540e\u7eed\u7684\u5185\u5bb9\u4e2d\u5206\u4eabIGV\u7684\u4f7f\u7528\u65b9\u5f0f\u54e6~<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5728\u4e4b\u524d\u7684\u6587\u7ae0\u4e2d\uff0c\u5c0f\u679c\u5411\u5927\u5bb6\u8be6\u7ec6\u5206\u4eab\u4e86\u8d28\u91cf\u8bc4\u4f30\u7684\u64cd\u4f5c\u8fc7\u7a0b\u548c\u7ed3\u679c\u5206\u6790\uff0c\u76f8\u4fe1\u5c0f\u4f19\u4f34\u4eec\u5df2\u7ecf\u719f\u7ec3\u638c\u63e1\uff0c\u8feb\u4e0d\u53ca\u5f85\u5730\u8fdb\u5165\u4e0b\u4e00 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/25295"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25295"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/25295\/revisions"}],"predecessor-version":[{"id":25301,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/25295\/revisions\/25301"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25295"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25295"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25295"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}