{"id":26820,"date":"2024-01-29T17:19:21","date_gmt":"2024-01-29T09:19:21","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=26820"},"modified":"2024-01-29T17:19:24","modified_gmt":"2024-01-29T09:19:24","slug":"seqkit-%e5%ba%8f%e5%88%97%e6%a2%b3%e7%90%86%e7%a5%9e%e5%99%a8","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=26820","title":{"rendered":"seqkit \u2014\u2014\u5e8f\u5217\u68b3\u7406\u795e\u5668"},"content":{"rendered":"

\u5c0f\u679c\u5728\u6700\u8fd1\u53d1\u73b0\u4e86\u4e00\u4e2a\u5904\u7406\u5e8f\u5217\u65f6\u5019\u53d1\u73b0\u4e86\u4e00\u4e2a\u597d\u7528\u7684\u751f\u4fe1\u5c0f\u5de5\u5177\u3002\u4eca\u5929\u5c0f\u679c\u628a\u5b83\u5206\u4eab\u7ed9\u4f60\u4eec\uff5e<\/p>\n

\u7b80\u4ecb<\/h2>\n

Seqkit \u662f\u4e00\u4e2a\u5feb\u901f\u3001\u9ad8\u6548\u3001\u529f\u80fd\u4e30\u5bcc\u7684\u751f\u7269\u4fe1\u606f\u5b66\u5de5\u5177\uff0c\u7528\u4e8e\u5904\u7406DNA\u3001RNA\u548c\u86cb\u767d\u8d28\u5e8f\u5217\u3002\u5b83\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u547d\u4ee4\u884c\u5de5\u5177\u548cAPI\u63a5\u53e3\uff0c\u53ef\u4ee5\u8fdb\u884c\u5e8f\u5217\u6587\u4ef6\u683c\u5f0f\u8f6c\u6362\u3001\u5e8f\u5217\u5904\u7406\u548c\u5206\u6790\u3001\u5e8f\u5217\u7edf\u8ba1\u7b49\u64cd\u4f5c\u3002<\/p>\n

\u9996\u5148\uff0cSeqkit \u652f\u6301\u5e38\u89c1\u7684\u5e8f\u5217\u6587\u4ef6\u683c\u5f0f\uff0c\u5982FASTA\u3001FASTQ\u3001GTF\u3001BED\u7b49\uff0c\u53ef\u4ee5\u65b9\u4fbf\u5730\u5b9e\u73b0\u4e0d\u540c\u6587\u4ef6\u683c\u5f0f\u4e4b\u95f4\u7684\u8f6c\u6362\u3002<\/p>\n

\u5176\u6b21\uff0c\u9664\u4e86\u57fa\u672c\u7684\u6587\u4ef6\u683c\u5f0f\u8f6c\u6362\u5916\uff0cSeqkit \u8fd8\u6709\u5f88\u591a\u5f3a\u5927\u7684\u529f\u80fd\u3002\u4f8b\u5982\uff0c\u5b83\u53ef\u4ee5\u5bf9\u5e8f\u5217\u8fdb\u884c\u4fee\u526a\u3001\u622a\u53d6\u3001\u8fc7\u6ee4\uff0c\u5e76\u652f\u6301\u5bf9\u5e8f\u5217\u957f\u5ea6\u3001GC\u542b\u91cf\u3001\u78b1\u57fa\u6bd4\u4f8b\u7b49\u8fdb\u884c\u7edf\u8ba1\u5206\u6790\u3002\u6b64\u5916\uff0c\u8fd8\u53ef\u4ee5\u6839\u636e\u53c2\u8003\u5e8f\u5217\u8fdb\u884c\u6bd4\u5bf9\u4e0e\u6bd4\u5bf9\u7ed3\u679c\u5904\u7406\u4ee5\u53ca\u8fdb\u884c\u5e8f\u5217\u4e92\u8865\u3001\u53cd\u5411\u4e92\u8865\u3001\u53cd\u5411\u4e92\u8865\u4e92\u8865\u7b49\u5e38\u7528\u64cd\u4f5c\u3002<\/p>\n

\u4e0b\u8f7d\u5b89\u88c5<\/h2>\n

1\u3001\u9875\u9762\u4e0b\u8f7d<\/p>\n

\u6700\u65b0\u7248\u53d1\u5e03\u9875\u9762\uff1ahttps:\/\/github.com\/shenwei356\/seqkit\/releases<\/a><\/p>\n

\u53ef\u4ee5\u590d\u5236\u4e0a\u8ff0\u7684\u8fde\u63a5\u4e2d\u9002\u914d\u81ea\u5df1\u7535\u8111\u7cfb\u7edf\u7684\u8fde\u63a5<\/p>\n

\u8fd9\u91cc\u4ee5Linux\u4e0a\u4e3a\u4f8b\u5b50<\/p>\n

wget https:\/\/github.com\/shenwei356\/seqkit\/releases\/download\/v2.5.0\/seqkit_linux_amd64.tar.gz<\/a><\/p>\n

tar -zxvf seqkit_linux_amd64.tar.gz<\/p>\n

\"\u622a\u5c4f2023-08-01<\/p>\n

\u4e0a\u8ff0\u56fe\u7247\u4e2d\u7eff\u8272\u7684\u5c31\u662f\u6211\u4eec\u89e3\u538b\u540e\u7684\u5c0f\u5de5\u5177\u4e86\uff0c\u53ef\u4ee5\u76f4\u63a5\u4f7f\u7528\u5566<\/p>\n

2\u3001conda\u5b89\u88c5<\/p>\n

conda install -c bioconda seqkit<\/p>\n

\u547d\u4ee4\u89e3\u6790<\/h2>\n

seqkit \u4e00\u5171\u670937\u4e2a\u53ef\u7528\u7684\u547d\u4ee4<\/p>\n

amplicon \u901a\u8fc7\u5f15\u7269\u68c0\u7d22\u6269\u589e\u5b50(\u6216\u5176\u5468\u56f4\u7684\u7279\u5b9a\u533a\u57df)<\/p>\n

bam \u68c0\u67e5\u548c\u5728\u7ebf\u7ed8\u5236BAM\u8bb0\u5f55\u6587\u4ef6\u7684\u76f4\u65b9\u56fe<\/p>\n

common \u901a\u8fc7id\/\u540d\u79f0\/\u5e8f\u5217\u67e5\u627e\u591a\u4e2a\u6587\u4ef6\u7684\u516c\u5171\u5e8f\u5217<\/p>\n

concat \u8fde\u63a5\u591a\u4e2a\u6587\u4ef6\u4e2d\u5177\u6709\u76f8\u540cID\u7684\u5e8f\u5217<\/p>\n

convert \u8f6c\u6362FASTQ\u8d28\u91cf\u7f16\u7801\u683c\u5f0f\uff1a\u652f\u6301\u683c\u5f0f\u5305\u62ec\uff1a\u6851\u683c\uff0cSolexa\u548cIllumina<\/p>\n

duplicate \u91cd\u590d\u5e8f\u5217N\u6b21<\/p>\n

faidx \u521b\u5efaFASTA\u7d22\u5f15\u6587\u4ef6\u5e76\u63d0\u53d6\u5b50\u5e8f\u5217<\/p>\n

fish \u4f7f\u7528\u5c40\u90e8\u6bd4\u5bf9\u5728\u8f83\u5927\u7684\u5e8f\u5217\u4e2d\u5bfb\u627e\u77ed\u5e8f\u5217<\/p>\n

fq2fa \u8f6c\u6362FASTQ\u5230FASTA<\/p>\n

fx2tab \u5c06FASTA\/Q\u8f6c\u6362\u4e3a\u8868\u683c\u683c\u5f0f(\u5305\u542b\u957f\u5ea6\/GC\u542b\u91cf\/GC\u504f\u597d)<\/p>\n

genautocomplete \u751f\u6210shell\u81ea\u52a8\u5b8c\u6210\u811a\u672c<\/p>\n

grep \u901a\u8fc7ID\/name\/sequence\/sequence motif\u641c\u7d22\u5e8f\u5217\uff0c\u5141\u8bb8\u9519\u914d<\/p>\n

head \u6253\u5370\u7b2c\u4e00\u6761\u5e8f\u5217<\/p>\n

help \u6253\u5370\u5e2e\u52a9\u4fe1\u606f<\/p>\n

locate \u5b9a\u4f4d\u5e8f\u5217\uff0c\u6216\u8005motifs\uff0c\u5141\u8bb8\u9519\u914d<\/p>\n

mutate \u7f16\u8f91\u5e8f\u5217(\u70b9\u7a81\u53d8\u3001\u63d2\u5165\u3001\u5220\u9664)<\/p>\n

pair \u5339\u914d\u53cc\u7aef\u5e8f\u5217\u6587\u4ef6<\/p>\n

range \u6253\u5370\u4e00\u4e2a\u8303\u56f4\u5185\u7684\u5e8f\u5217<\/p>\n

rename \u91cd\u547d\u540d\u91cd\u590d\u5e8f\u5217ID<\/p>\n

replace \u4f7f\u7528\u6b63\u5219\u8868\u8fbe\u5f0f\u4fee\u6539\u540d\u79f0\u6216\u8005\u5e8f\u5217<\/p>\n

restart \u91cd\u7f6e\u73af\u72b6\u57fa\u56e0\u7ec4\u7684\u8d77\u59cb\u4f4d\u7f6e<\/p>\n

rmdup \u901a\u8fc7id\/\u540d\u79f0\/\u5e8f\u5217\u5220\u9664\u91cd\u590d\u7684\u5e8f\u5217<\/p>\n

sample \u6309\u6570\u91cf\u6216\u6bd4\u4f8b\u5bf9\u5e8f\u5217\u8fdb\u884c\u62bd\u6837<\/p>\n

sana \u6e05\u7406\u635f\u574f\u7684\u5355\u884cfastq\u6587\u4ef6<\/p>\n

scat real time recursive concatenation and streaming of fastx files<\/p>\n

seq \u8f6c\u6362\u5e8f\u5217(\u53cd\u5411\uff0c\u8865\u5145\uff0c\u63d0\u53d6ID\u2026)<\/p>\n

shuffle \u968f\u673a\u5e8f\u5217<\/p>\n

sliding \u5e8f\u5217\u6ed1\u7a97\u63d0\u53d6\uff0c\u652f\u6301\u73af\u5f62\u57fa\u56e0\u7ec4<\/p>\n

sort \u6309id\/\u540d\u79f0\/\u5e8f\u5217\/\u957f\u5ea6\u6392\u5e8f\u5e8f\u5217<\/p>\n

split \u6309id\/seq\u533a\u57df\/\u5927\u5c0f\/\u90e8\u4ef6\u5c06\u5e8f\u5217\u62c6\u5206\u4e3a\u6587\u4ef6(\u4e3b\u8981\u7528\u4e8eFASTA)<\/p>\n

split2 \u6309\u5e8f\u5217\u6570\u91cf\/\u6587\u4ef6\u6570\u5c06\u5e8f\u5217\u62c6\u5206\u4e3a\u591a\u4e2a\u6587\u4ef6(FASTA, PE\/SE FASTQ)<\/p>\n

stats FASTA\/Q\u6587\u4ef6\u7684\u7b80\u5355\u7edf\u8ba1<\/p>\n

subseq \u901a\u8fc7region\/gtf\/bed\u5f97\u5230\u5b50\u5e8f\u5217\uff0c\u5305\u62ec\u4fa7\u7ffc\u5e8f\u5217<\/p>\n

tab2fx \u8f6c\u6362\u8868\u683c\u683c\u5f0f\u4e3aFASTA\/Q\u683c\u5f0f<\/p>\n

translate \u7ffb\u8bd1DNA\/RNA\u5230\u86cb\u767d\u8d28\u5e8f\u5217(\u652f\u6301\u6b67\u4e49\u78b1\u57fa)<\/p>\n

version \u6253\u5370\u7248\u672c\u4fe1\u606f\u5e76\u68c0\u67e5\u662f\u5426\u66f4\u65b0<\/p>\n

watch \u5e8f\u5217\u7279\u5f81\u7684\u76d1\u6d4b\u548c\u5728\u7ebf\u76f4\u65b9\u56fe<\/p>\n

\u5b9e\u8df5\u64cd\u4f5c<\/h2>\n

\u56e0\u4e3asepkit\u7684\u5de5\u5177\u592a\u5f3a\u5927\u4e86\uff0c\u8fd9\u91cc\u5217\u4e3e\u4e00\u4e9b\u6211\u4eec\u5e38\u7528\u5e38\u89c1\u7684\u64cd\u4f5c<\/p>\n

\u4e0b\u8f7d\u4e24\u4e2a\u5e8f\u5217\u505a\u4e3a\u793a\u4f8b\u64cd\u4f5c\uff0c\u4e00\u4e2afastq\u6587\u4ef6\uff0c\u4e00\u4e2afasta\u6587\u4ef6<\/p>\n

wget http:\/\/data.biostarhandbook.com\/reads\/duplicated-reads.fq.gz<\/p>\n

wget ftp:\/\/ftp.ncbi.nih.gov\/refseq\/release\/viral\/viral.1.1.genomic.fna.gz<\/p>\n

stats \u7b80\u5355\u7edf\u8ba1<\/h2>\n

#\u7edf\u8ba1\u5e8f\u5217\u683c\u5f0ffasta(fa)\/fastq(fq)\u3001\u5185\u5bb9\u7c7b\u578bDNA\/RNA\/Protein\uff0c\u5e8f\u5217\u6570\u91cf\u3001\u603b\u957f\u5ea6\uff0c\u6700\u5c0f\u3001\u5e73\u5747\u548c\u6700\u5927\u957f\u5ea6<\/p>\n

# FASTA DNA<\/p>\n

seqkit stats viral.1.1.genomic.fna.gz<\/p>\n

\"\u622a\u5c4f2023-08-01<\/p>\n

# FASTQ DNA<\/p>\n

seqkit stats duplicate.fastq<\/p>\n

\"\u622a\u5c4f2023-08-01<\/h2>\n

seq \u5e8f\u5217\u64cd\u4f5c<\/h2>\n

\u201c-n\u201d: \u63d0\u53d6\u5e8f\u5217ID\uff0c\u5305\u62ec\u201c>\u201d\u540e\u9762\u7684\u5168\u90e8\u5185\u5bb9<\/h3>\n

seqkit seq -n viral.1.1.genomic.fna.gz |head<\/p>\n

\"\u622a\u5c4f2023-08-01<\/p>\n

\u201c-n -i\u201d: \u4ec5\u63d0\u53d6\u7b2c\u4e00\u4e2a\u7a7a\u683c\u524d\u7684ID<\/h3>\n

seqkit seq -n -i viral.1.1.genomic.fna.gz |head<\/p>\n

\"\u622a\u5c4f2023-08-01<\/p>\n

\u63d0\u53d6\u5e8f\u5217\u957f\u5ea6\u5927\u4e8e50\u7684\u5e76\u7edf\u8ba1\u957f\u5ea6\u4fe1\u606f<\/h3>\n

seqkit seq -m 50 viral.1.1.genomic.fna.gz |seqkit stats<\/p>\n

\"\u622a\u5c4f2023-08-01<\/p>\n

\u8bbe\u7f6e\u6700\u5c0f\u5e8f\u5217\u957f\u5ea6\u548c\u6700\u5927\u5e8f\u5217\u957f\u5ea6\uff0c\u7528\u4e8e\u8fc7\u6ee4\u5e8f\u5217\uff0c\u5e76\u7edf\u8ba1<\/h3>\n

seqkit seq -m 100 -M 1000 viral.1.1.genomic.fna.gz<\/p>\n

    \n
  1. \u4fdd\u5b58>100\u4e14<1000\u957f\u5ea6\u7684\u5e8f\u5217<\/li>\n<\/ol>\n

    seqkit seq -m 100 -M 1000 viral.1.1.genomic.fna.gz >a_100_1000.fa<\/p>\n

    \u5355\u884c\/\u591a\u884c\u8f6c\u6362<\/h3>\n

    -s\u63d0\u53d6\u5e76\u5c55\u793a\u5e8f\u5217<\/p>\n

    -w \u4ee3\u8868\u6bcf\u884c\u7684\u78b1\u57fa\u6570\u91cf\uff0c0\u4ee3\u8868\u4e0d\u6362\u884c<\/p>\n

    seqkit seq viral.1.1.genomic.fna.gz -s -w 0 |head<\/p>\n

    -r \u5e8f\u5217\u53cd\u5411 -p\u5e8f\u5217\u4e92\u8865<\/h3>\n

    # \u5e8f\u5217\u53cd\u5411\u4e92\u8865,-r\u53cd\u5411\uff0c-p\u4e92\u8865<\/p>\n

    echo -e “>123\\nATCG”|seqkit seq -r -p<\/p>\n

    \"\u622a\u5c4f2023-08-01<\/p>\n

    \u5220\u9664gap\/\u5927\u5c0f\u5199\u8f6c\u6362<\/h3>\n

    -g \u53bb\u9664\u5e8f\u5217\u4e2d\u7684\u95f4\u9694,\u5c06\u4e2d\u95f4\u7684\u6a2a\u6760\u53bb\u6389<\/p>\n

    -u\u8f6c\u5316\u5e8f\u5217\u4e3a\u5927\u5199\u5b57\u6bcd\u5c55\u793a<\/p>\n

    echo -e “>seq\\nACGT-ACTGC-acc” | seqkit seq -g -u<\/p>\n

    \"\u622a\u5c4f2023-08-01<\/p>\n

    \u2014rna2dna \u5c06RNA\u5e8f\u5217\u8f6c\u5316\u4e3aDNA\u5e8f\u5217<\/h3>\n

    echo -e “>seq\\nUCAUAUGCUUGUCUCAAAGAUUA” | seqkit seq –rna2dna<\/p>\n

    \"\u622a\u5c4f2023-08-01<\/h1>\n

    subseq\u6307\u5b9a\u533a\u57df<\/h2>\n

    -r \u901a\u8fc7\u533a\u57df\u6765\u622a\u53d6\u5e8f\u52172:10\u63d0\u53d62\u523010\u4e2a\u78b1\u57fa\uff0c-12:-1\u63d0\u53d6\u5e8f\u5217\u7ed3\u5c3e12\u4e2a\u78b1\u57fa\uff1b<\/p>\n

    seqkit subseq -r 2:10 viral.1.1.genomic.fna.gz |head -2<\/p>\n

    \"\u622a\u5c4f2023-08-01<\/p>\n

    fq2fa \u5c06fq\u8f6c\u4e3afa\u683c\u5f0f<\/h2>\n

    seqkit fq2fa duplicate.fastq -o duplicate.fa<\/p>\n

    head duplicate.fa<\/p>\n

    \"\u622a\u5c4f2023-08-01<\/p>\n

    fx2tab & tab2fx \u5e8f\u5217\u8f6c\u5316\u8868\u683c\u683c\u5f0f<\/h2>\n

    fx2tab<\/p>\n

    \u8fd9\u4e00\u8f6c\u5316\u975e\u5e38\u7684\u6709\u7528\uff0c\u5f80\u5f80\u7528\u4e8e\u8868\u683c\/\u77e9\u9635\u5904\u7406\u7684\u65f6\u5019<\/p>\n

    seqkit fx2tab viral.1.1.genomic.fna.gz | less -S<\/p>\n

    # \u6253\u5370\u5e8f\u5217\u957f\u5ea6\u3001GC\u542b\u91cf<\/p>\n

    seqkit fx2tab viral.1.1.genomic.fna.gz -l -g -n -i -H | head<\/p>\n

    \"\u622a\u5c4f2023-08-01<\/p>\n

    #\u901a\u8fc7\u77e9\u9635\u683c\u5f0f\u7684\u5e8f\u5217\u6587\u4ef6\u7edf\u8ba1\u5e8f\u5217\u957f\u5ea6\u548c\u8d28\u91cf\u503c<\/p>\n

    -l \u7edf\u8ba1\u5e8f\u5217\u957f\u5ea6<\/p>\n

    -g \u7edf\u8ba1\u5e73\u5747GC\u542b\u91cf<\/p>\n

    -i \u53ea\u6253\u5370\u540d\u79f0(\u4e0d\u6253\u5370\u5e8f\u5217)<\/p>\n

    -H \u6253\u5370\u6807\u9898\u884c<\/p>\n

    tab2fx<\/p>\n

    seqkit fx2tab viral.1.1.genomic.fna.gz |seqkit tab2fx |head<\/p>\n

    \"\u622a\u5c4f2023-08-01<\/p>\n

    translate \u7ffb\u8bd1DNA\/RNA\u4e3a\u86cb\u767d\u8d28\u5e8f\u5217<\/h2>\n

    seqkit translate viral.1.1.genomic.fna.gz |head<\/p>\n

    \"\u622a\u5c4f2023-08-02<\/p>\n

    faidx \u5efa\u7acb\u7d22\u5f15\u6587\u4ef6\u3001\u63d0\u53d6\u5b50\u5e8f\u5217<\/h2>\n

    seqkit faidx viral.1.1.genomic.fna<\/p>\n

    seqkit faidx viral.1.1.genomic.fna NC_030200.1 #\u63d0\u53d6ID\u4fe1\u606f<\/p>\n

    seqkit faidx viral.1.1.genomic.fna NC_030200.1 # -f \u6807\u9898\u5168\u90e8\u8f93\u51fa<\/p>\n

    seqkit faidx viral.1.1.genomic.fna NC_030200.1:1-10 #\u63d0\u53d6\u5b50\u5e8f\u5217\u7b2c1\u4e2a\u5230\u7b2c10\u4e2a\u78b1\u57fa<\/p>\n

    \"\u622a\u5c4f2023-08-02<\/p>\n

    grep \u5339\u914d<\/h2>\n

    seqkit grep -f id.txt viral.1.1.genomic.fna -o result.fq.gz#\u6309 ID \u5217\u8868\u6587\u4ef6\u641c\u7d22\uff08\u4e0d\u5305\u542b\u7a7a\u683c\uff09<\/p>\n

    \"\u622a\u5c4f2023-08-02<\/p>\n

    seqkit grep -n -f name.txt viral.1.1.genomic.fna -o result.fa.gz #\u4f7f\u7528\u5e8f\u5217\u540d\u79f0\u5217\u8868\u8fdb\u884c\u641c\u7d22<\/p>\n

    cat viral.1.1.genomic.fna | seqkit grep -s -i -p aggcg #\u63d0\u53d6\u5305\u542b AGGCG \u7684\u5e8f\u5217<\/p>\n

    zcat viral.1.1.genomic.fna | seqkit grep -s -r -i -p ^aggcg #\u63d0\u53d6\u4ee5 AGGCG \u5f00\u5934\u7684\u5e8f\u5217<\/p>\n

    #-i\u53c2\u6570\u5ffd\u7565\u5927\u5c0f\u5199<\/p>\n

    spilt \u62c6\u5206<\/h2>\n

    seqkit split viral.1.1.genomic.fna -s 10000 #\u5c06\u5e8f\u5217\u62c6\u5206\u4e3a\u6309\u716710000\u4e2a\u5e8f\u5217\u7684\u90e8\u5206<\/p>\n

    seqkit split viral.1.1.genomic.fna -p 4 #\u5c06\u5e8f\u5217\u62c6\u5206\u4e3a4\u90e8\u5206<\/p>\n

    seqkit split viral.1.1.genomic.fna -p 4 -2#\u52a0\u4e0a-2\u51cf\u5c11\u5185\u5b58\u4f7f\u7528<\/p>\n

    seqkit split viral.1.1.genomic.fna -i –id-regexp “^([\\w]+)\\-” -2 #\u6309id\u62c6\u5206\u5e8f\u5217<\/p>\n

    seqkit split viral.1.1.genomic.fna -r 1:3 -2 #\u6309\u524d\u4e09\u4e2a\u5e8f\u5217\u78b1\u57fa\u6765\u533a\u5206<\/p>\n

    \"\/private\/var\/folders\/xd\/jn2f48nj2k52zzgd8wm1j4yr0000gn\/T\/com.kingsoft.wpsoffice.mac\/photoedit2\/20230802224807\/temp.pngtemp\"<\/p>\n

    seqkit\u5c0f\u5de5\u5177\u9690\u85cf\u5f88\u591a\u597d\u7528\u7684\u529f\u80fd\uff0c\u9700\u8981\u6211\u4eec\u4e0d\u65ad\u5c1d\u8bd5\uff0c\u5c0f\u679c\u628a\u8f6f\u4ef6\u7684github\u8fde\u63a5\u7ed9\u4f60\u4eec<\/p>\n

    https:\/\/github.com\/shenwei356\/seqkit<\/a>\uff0c\u5e0c\u671b\u4f60\u9047\u5230\u6709\u5173\u5904\u7406\u6570\u636e\u7684\u95ee\u9898\uff0c\u53ef\u4ee5\u5c1d\u8bd5\u7528\u8fd9\u4e2a\u4e07\u80fd\u5c0f\u5de5\u5177\u6765\u89e3\u51b3\uff5e<\/p>\n

    \u5982\u679c\u9047\u5230\u4e0d\u61c2\u7684\u4e5f\u53ef\u4ee5\u501f\u52a9\u7ebf\u4e0a\u7684\u4e91\u5e73\u53f0\u54e6\uff5ehttp:\/\/www.biocloudservice.com\/home.html<\/p>\n

    \u597d\u4e86\u4eca\u5929\u7684seqkit\u5c0f\u8f6f\u4ef6\u5c31\u8bb2\u5230\u8fd9\u91cc\uff0c\u6b22\u8fce\u5927\u5bb6\u6709\u95ee\u9898\u4e0e\u5c0f\u679c\u4e00\u8d77\u8ba8\u8bba\u54e6\uff5e<\/p>\n","protected":false},"excerpt":{"rendered":"

    \u5c0f\u679c\u5728\u6700\u8fd1\u53d1\u73b0\u4e86\u4e00\u4e2a\u5904\u7406\u5e8f\u5217\u65f6\u5019\u53d1\u73b0\u4e86\u4e00\u4e2a\u597d\u7528\u7684\u751f\u4fe1\u5c0f\u5de5\u5177\u3002\u4eca\u5929\u5c0f\u679c\u628a\u5b83\u5206\u4eab\u7ed9\u4f60\u4eec\uff5e \u7b80\u4ecb Seqkit \u662f\u4e00 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/26820"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26820"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/26820\/revisions"}],"predecessor-version":[{"id":26838,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/26820\/revisions\/26838"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26820"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26820"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26820"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}