{"id":29745,"date":"2024-02-26T17:34:28","date_gmt":"2024-02-26T09:34:28","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=29745"},"modified":"2024-02-26T17:34:29","modified_gmt":"2024-02-26T09:34:29","slug":"sccancer-%e4%bd%a0%e7%9a%84%e8%82%bf%e7%98%a4%e5%8d%95%e7%bb%86%e8%83%9e%e5%8c%85%e8%bd%ac%e5%bd%95%e7%bb%84%e8%a1%a8%e5%88%86%e6%9e%90%e5%b8%88%ef%bc%81","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=29745","title":{"rendered":"scCancer&#8211;\u4f60\u7684\u80bf\u7624\u5355\u7ec6\u80de\u5305\u8f6c\u5f55\u7ec4\u8868\u5206\u6790\u5e08\uff01"},"content":{"rendered":"<h1>scCancer&#8211;\u4f60\u7684\u80bf\u7624\u5355\u7ec6\u80de\u5305\u8f6c\u5f55\u7ec4\u8868\u5206\u6790\u5e08\uff01<\/h1>\n<p>\u4f60\u77e5\u9053\u6709\u4e00\u4e2a\u5de5\u5177\u53ef\u4ee5\u5e2e\u52a9\u4f60\u5206\u6790\u80bf\u7624\u5355\u7ec6\u80de\u7684\u8f6c\u5f55\u7ec4\u5417\uff1f\u751a\u81f3\u8fd9\u4e2a\u5de5\u5177\u53ef\u4ee5\u76f4\u63a5\u5e2e\u4f60\u751f\u6210\u5206\u6790\u62a5\u8868\u561b\uff1f\u4eca\u5929\u5c0f\u679c\u5c31\u5e26\u5927\u5bb6\u6765\u8ba4\u8bc6\u8fd9\u4e2a\u64cd\u4f5c\u7b80\u5355\uff0c\u6548\u7387\u53c8\u6781\u9ad8\u7684R\u5de5\u5177\u5305\uff0c\u5b66\u4f1a\u540e\u53ef\u4ee5\u76f4\u63a5\u6210\u4e3a\u4f60\u7684\u5206\u6790\u52a9\u7406\u54e6\uff01\u73b0\u5728\u5927\u5bb6\u5c31\u8ddf\u7740\u5c0f\u679c\u4e00\u8d77\u6765\u770b\u770b\u5427\uff01<\/p>\n<p><strong>\u5b89\u88c5scCancer<\/strong><\/p>\n<p>\u662f\u7684\u6ca1\u9519\uff0c\u4eca\u5929\u8981\u5e26\u5927\u5bb6\u8ba4\u8bc6\u5e76\u5b66\u4e60\u7684R\u5de5\u5177\u5305\u5c31\u662fscCancer\uff0c\u9996\u5148\u8ba9\u6211\u4eec\u6765\u4e00\u8d77\u5b66\u4e60\u4e0b\u5982\u4f55\u5b89\u88c5\u5427\uff01\u5c0f\u679c\u5728\u8fd9\u91cc\u6574\u7406\u4e86\u4e24\u79cd\u5b89\u88c5\u65b9\u6cd5\uff0c\u5404\u4f4d\u540c\u978b\u6839\u636e\u81ea\u5df1\u7684\u60c5\u51b5\u81ea\u884c\u9009\u62e9\u4e0b\u8f7d\u54e6\uff01<\/p>\n<ul>\n<li>\u901a\u8fc7Bioconductor\u4e0b\u8f7d<\/li>\n<\/ul>\n<p>BiocManager::install(&#8216;scCancer&#8217;)<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"51\" class=\"wp-image-29747\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-29745-1.png?resize=640%2C51\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-29745-1.png?w=985 985w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-29745-1.png?resize=300%2C24 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-29745-1.png?resize=768%2C61 768w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-29745-1.png?resize=600%2C48 600w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/> \u76f4\u63a5\u901a\u8fc7Bioconductor\u4e0b\u8f7d\u7684\u540c\u5b66\u8981\u6ce8\u610f\u5566\uff01\u8fd9\u6837\u4e0b\u8f7d\u5bf9\u4f60\u7684R\u7248\u672c\u662f\u6709\u8981\u6c42\u7684\u54e6\uff0c\u5982\u679c\u7248\u672c\u4e0d\u5339\u914d\u4f1a\u62a5\u4ee5\u4e0b\u9519\u8bef\u4fe1\u606f\uff0c\u5c0f\u679c\u7b2c\u4e00\u6b21\u5728\u4e0b\u8f7d\u7684\u65f6\u5019\u5c31\u9047\u5230\u4e86\u8fd9\u6837\u7684\u95ee\u9898\u3002<\/p>\n<p>\u6240\u4ee5\uff0c\u5c0f\u679c\u6709\u627e\u5230\u4e00\u79cd\u66f4\u4e0d\u5bb9\u6613\u51fa\u9519\u7684\u4e0b\u8f7d\u65b9\u6cd5\uff1a<\/p>\n<ul>\n<li>\u901a\u8fc7github\u4e0b\u8f7d<\/li>\n<\/ul>\n<p>install_github(&#8216;linxihui\/NNLM&#8217;)<br \/>\ninstall_github(&#8220;wguo-research\/scCancer&#8221;)<\/p>\n<p>\u8fd9\u91cc\u5c0f\u679c\u6e29\u99a8\u63d0\u793a\uff0c\u901a\u8fc7gihub\u4e0b\u8f7d\u4e4b\u524d\u8981\u901a\u8fc7github\u5148\u4e0b\u8f7d\u597dNNLM\u5305\u54e6\uff0c\u4e0d\u7136\u4e0b\u8f7dscCancer\u5305\u7684\u8fc7\u7a0b\u4e5f\u4f1a\u62a5\u9519\u5bfc\u81f4\u65e0\u6cd5\u6b63\u5e38\u5b8c\u6210\u4e0b\u8f7d\u54e6\uff01<\/p>\n<p><strong>scCancer\u81ea\u52a8\u751f\u6210\u80bf\u7624\u7ec6\u80de\u5206\u6790\u62a5\u8868<\/strong><\/p>\n<ul>\n<li><strong>\u4e0b\u8f7d\u5355\u6837\u672c\u6570\u636e<\/strong><\/li>\n<\/ul>\n<p>\u5728\u8fdb\u5165\u6211\u4eec\u5e26\u7684\u5206\u6790\u6d41\u7a0b\u4e4b\u524d\uff0c\u5c0f\u679c\u4e3a\u5927\u5bb6\u8d34\u51fa\u4ee5\u4e0b\u6570\u636e\u8fde\u63a5\u4f5c\u4e3a\u6211\u4eec\u5206\u6790\u7684\u53c2\u8003\u6570\u636e\uff1a<a href=\"http:\/\/lifeome.net\/software\/sccancer\/KC-example.tar.gz\">http:\/\/lifeome.net\/software\/sccancer\/KC-example.tar.gz<\/a><\/p>\n<p>\u540c\u5b66\u4eec\u8d76\u7d27\u8ddf\u7740\u5c0f\u679c\u4e00\u8d77\u4e0b\u8f7d\u8d77\u6765\u5427\uff01~~~<\/p>\n<p>\u4e0b\u8f7d\u597d\u540e\uff0c\u540c\u5b66\u4eec\u8981\u628a\u8fd9\u4e9b\u6570\u636e\u89e3\u538b\u5728\u81ea\u5df1\u5408\u9002\u7684\u5de5\u4f5c\u76ee\u5f55\u4e0b\u54e6\uff0c\u5e76\u6253\u5f00\u6211\u4eec\u7684\u6570\u636e\u6587\u4ef6\u5939\uff0c\u4e0b\u9762\u5c0f\u679c\u6765\u5e26\u7740\u5927\u5bb6\u4e00\u8d77\u8ba4\u8bc6\u4e00\u4e0b\u8fd9\u4e9b\u6570\u636e\u7684\u4e3b\u8981\u5185\u5bb9\u5427\uff01<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"384\" height=\"63\" class=\"wp-image-29748\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-29745-2.png?resize=384%2C63\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-29745-2.png?w=384 384w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-29745-2.png?resize=300%2C49 300w\" sizes=\"(max-width: 384px) 100vw, 384px\" data-recalc-dims=\"1\" \/><\/p>\n<p>&gt; list.files(&#8220;.\/sample&#8221;,recursive = T)<br \/>\n[1] &#8220;KC-example\/filtered_feature_bc_matrix\/barcodes.tsv.gz&#8221;<br \/>\n[2] &#8220;KC-example\/filtered_feature_bc_matrix\/features.tsv.gz&#8221;<br \/>\n[3] &#8220;KC-example\/filtered_feature_bc_matrix\/matrix.mtx.gz&#8221;<br \/>\n[4] &#8220;KC-example\/raw_feature_bc_matrix\/barcodes.tsv.gz&#8221;<br \/>\n[5] &#8220;KC-example\/raw_feature_bc_matrix\/features.tsv.gz&#8221;<br \/>\n[6] 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\u4ee3\u8868\u8fc7\u6ee4\u540e\u7684\u5355\u7ec6\u80de\u8868\u8fbe\u6570\u636e\u3002<\/p>\n<ul>\n<li><strong>\u5f00\u59cb\u5206\u6790<\/strong><\/li>\n<\/ul>\n<p>\u73b0\u5728\u6211\u4eec\u5b8c\u6210\u4e86R\u5305\u7684\u5b89\u88c5\u548c\u6570\u636e\u96c6\u7684\u5bfc\u5165\uff0c\u5c0f\u679c\u8981\u544a\u8bc9\u4f60\u7684\u662f\uff0c\u5176\u5b9e\u6211\u4eec\u5df2\u7ecf\u5b8c\u6210\u4e86\u4e00\u5927\u90e8\u5206\u5de5\u4f5c\uff0c\u56e0\u4e3ascCancer\u53ef\u4ee5\u81ea\u5df1\u5b8c\u6210\u80bf\u7624\u7ec6\u80de\u7684\u5168\u5206\u6790\u6d41\u7a0b\uff0c\u5e76\u4e3a\u6211\u4eec\u751f\u6210\u5b8c\u6574\u7684\u5206\u6790\u62a5\u8868\u5728\u6211\u4eec\u7684\u7ed3\u679c\u76ee\u5f55\u4e2d\uff0c\u6211\u4eec\u63a5\u4e0b\u6765\u8981\u505a\u7684\u5c31\u662f\u914d\u7f6e\u6240\u6709\u7684\u6570\u636e\u5408\u4fdd\u5b58\u8def\u5f84\u5373\u53ef\uff0c\u63a5\u4e0b\u6765\u6211\u4eec\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\u5982\u4f55\u64cd\u4f5c\u5427\uff01<\/p>\n<p>dataPath &lt;- &#8220;.\/data\/sample&#8221; \u00a0 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class=\"wp-image-29750\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-29745-4.png?resize=640%2C513\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-29745-4.png?w=1305 1305w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-29745-4.png?resize=300%2C241 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-29745-4.png?resize=1024%2C822 1024w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-29745-4.png?resize=768%2C616 768w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-29745-4.png?resize=600%2C481 600w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"473\" 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data-recalc-dims=\"1\" \/><\/p>\n<p>\u597d\u5566\uff0c\u4eca\u5929\u7684\u5c0f\u5de5\u5177\u4f60\u5b66\u4f1a\u4e86\u561b\uff1f<\/p>\n<p>\u66f4\u591a\u751f\u4fe1\u5de5\u5177\u7684\u5b66\u4e60\u656c\u8bf7\u7ee7\u7eed\u5173\u6ce8\u5c0f\u679c\u54e6\uff01\uff01<\/p>\n","protected":false},"excerpt":{"rendered":"<p>scCancer&#8211;\u4f60\u7684\u80bf\u7624\u5355\u7ec6\u80de\u5305\u8f6c\u5f55\u7ec4\u8868\u5206\u6790\u5e08\uff01 \u4f60\u77e5\u9053\u6709\u4e00\u4e2a\u5de5\u5177\u53ef\u4ee5\u5e2e\u52a9\u4f60\u5206\u6790\u80bf\u7624\u5355\u7ec6\u80de\u7684\u8f6c\u5f55 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/29745"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=29745"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/29745\/revisions"}],"predecessor-version":[{"id":29758,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/29745\/revisions\/29758"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=29745"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=29745"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=29745"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}