{"id":30037,"date":"2024-02-27T18:06:49","date_gmt":"2024-02-27T10:06:49","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=30037"},"modified":"2024-02-27T18:06:51","modified_gmt":"2024-02-27T10:06:51","slug":"%e5%92%8cr%e8%af%ad%e8%a8%80%e4%b8%80%e8%b5%b7%e6%8e%a2%e7%b4%a2%e5%8d%95%e7%bb%86%e8%83%9e%e5%9f%ba%e5%9b%a0%e8%a1%a8%e8%be%be%ef%bc%81","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=30037","title":{"rendered":"\u548cR\u8bed\u8a00\u4e00\u8d77\u63a2\u7d22\u5355\u7ec6\u80de\u57fa\u56e0\u8868\u8fbe\uff01"},"content":{"rendered":"<p><strong>\u548cR\u8bed\u8a00\u4e00\u8d77\u63a2\u7d22\u5355\u7ec6\u80de\u57fa\u56e0\u8868\u8fbe\uff01<\/strong><\/p>\n<p>\u5f53\u6211\u4eec\u62ff\u5230\u4e00\u7ec4\u5355\u7ec6\u80de\u6570\u636e\u7684\u77e9\u9635\u65f6\uff0c\u5982\u4f55\u5728\u5904\u7406\u4e4b\u524d\u89c2\u5bdf\u7ec6\u80de\u548c\u57fa\u56e0\u8868\u8fbe\u4e4b\u95f4\u7684\u5173\u7cfb\u5462\uff1f\u4eca\u5929\u5c0f\u679c\u5c31\u5e26\u5927\u5bb6\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\uff01<\/p>\n<ul>\n<li><strong>\u751f\u6210\u6570\u636e\u77e9\u9635<\/strong><\/li>\n<\/ul>\n<p>\u4eca\u5929\u6211\u4eec\u7528\u5230\u7684\u6570\u636e\u4ecd\u7136\u6765\u81ea\u4e8escRNAseq\u5305\u4e2d\u7684\u7ec6\u80de\u96c6\u54e6\uff01\u6570\u636e\u77e9\u9635\u7684\u751f\u6210\u65b9\u6cd5\u5c0f\u679c\u5df2\u7ecf\u7ed9\u5927\u5bb6\u51c6\u5907\u597d\u5566\uff01\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\u5427\uff01<\/p>\n<p>library(scRNAseq)<br \/>\ndata(fluidigm)<br \/>\nfluidigm&lt;-ReprocessedFluidigmData()<br \/>\nct &lt;- floor(assays(fluidigm)$rsem_counts) #\u751f\u6210\u6570\u636e\u77e9\u9635<br \/>\nct[1:4,1:4]<br \/>\nsample_ann &lt;- as.data.frame(colData(fluidigm))<br \/>\nDT::datatable(sample_ann)<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"298\" class=\"wp-image-30038\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/unnamed-file-107.png?resize=640%2C298\" alt=\"\u8868\u683c\n\n\u63cf\u8ff0\u5df2\u81ea\u52a8\u751f\u6210\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/unnamed-file-107.png?w=1927 1927w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/unnamed-file-107.png?resize=300%2C140 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/unnamed-file-107.png?resize=1024%2C477 1024w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/unnamed-file-107.png?resize=768%2C357 768w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/unnamed-file-107.png?resize=1536%2C715 1536w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/unnamed-file-107.png?resize=600%2C279 600w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/unnamed-file-107.png?w=1280 1280w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/> \u4f7f\u7528R\u8bed\u8a00\u4e2d\u7684View()\u51fd\u6570\u53ef\u4ee5\u76f4\u63a5\u67e5\u770b\u6211\u4eec\u751f\u6210\u7684\u6570\u636e\u77e9\u9635\u54e6\uff0c\u6211\u4eec\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\u3002<\/p>\n<ul>\n<li><strong>\u63a2\u7d22\u57fa\u56e0\u8868\u8fbe\u60c5\u51b5<\/strong><\/li>\n<\/ul>\n<p>\u5728\u63a2\u7d22\u57fa\u56e0\u8868\u8fbe\u60c5\u51b5\uff0c\u5177\u4f53\u65f6\u4ece\u54ea\u4e9b\u89d2\u5ea6\u6765\u770b\u5462\uff1f\u5c0f\u679c\u5728\u8fd9\u91cc\u7ed9\u5927\u5bb6\u4f53\u5171\u4e24\u79cd\u601d\u8def\uff1a<\/p>\n<p>1.\u7edf\u8ba1\u67d0\u4e2a\u57fa\u56e0\u5728\u591a\u5c11\u4e2a\u7ec6\u80de\u4e2d\u5f97\u5230\u8868\u8fbe\uff1b<\/p>\n<p>2.\u7edf\u8ba1\u4e00\u4e2a\u7ec6\u80de\u4e2d\u8868\u8fbe\u4e86\u591a\u5c11\u4e2a\u57fa\u56e0\uff1b<\/p>\n<p>\u90a3\u4e48\u8981\u4ece\u8fd9\u4e24\u4e2a\u89d2\u5ea6\u53bb\u63a2\u7d22\uff0c\u5206\u522b\u8981\u5982\u4f55\u53bb\u7edf\u8ba1\u5462\uff1f\u5feb\u8c03\u52a8\u4f60\u806a\u660e\u7684\u5c0f\u8111\u888b\uff01\u5982\u679c\u60f3\u4e0d\u5230\u4e5f\u6ca1\u5173\u7cfb\uff0c\u4eca\u5929\u5c0f\u679c\u624b\u628a\u624b\u6559\u4f60\u5b8c\u6210\uff01<\/p>\n<ul>\n<li>\n<ul>\n<li><strong>\u7edf\u8ba1\u6240\u6709\u57fa\u56e0\u8868\u8fbe\u6210\u529f\u7684\u7ec6\u80de\u4e2a\u6570<\/strong><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>\u5bf9\u4e8e\u57fa\u56e0\u8868\u8fbe\u6210\u529f\u7684\u7ec6\u80de\u4e2a\u6570\uff0c\u6211\u4eec\u9700\u8981\u7528apply\u51fd\u6570\u7edf\u8ba1\u6570\u636e\u77e9\u9635\u4e2d\u6bcf\u4e00\u884c\u5143\u7d20\u4e2d\u5bf9\u5e94\u503c\u5927\u4e8e0\u7684\u4e2a\u6570\uff0c\u63a5\u4e0b\u6765\u6211\u4eec\u8fdb\u884c\u7edf\u8ba1\u5e76\u7ed8\u5236\u7bb1\u7ebf\u56fe\u89c2\u5bdf\u6574\u4f53\u8868\u8fbe\u60c5\u51b5\u3002\u548c\u5c0f\u679c\u4e00\u8d77\u6765\u770b\u770b\u5427\uff01<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"466\" class=\"wp-image-30039\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-2.png?resize=640%2C466\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-2.png?w=2460 2460w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-2.png?resize=300%2C219 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-2.png?resize=1024%2C746 1024w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-2.png?resize=768%2C559 768w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-2.png?resize=1536%2C1119 1536w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-2.png?resize=2048%2C1492 2048w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-2.png?resize=600%2C437 600w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-2.png?w=1280 1280w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-2.png?w=1920 1920w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/> fivenum(apply(counts,1,function(x) sum(x&gt;0) ))#\u7edf\u8ba1\u6240\u6709\u57fa\u56e0\u8868\u8fbe\u6210\u529f\u7ec6\u80de\u4e2a\u6570\u4e2d\u7684\u4e94\u4e2a\u6307\u6807<br \/>\nboxplot(apply(counts,1,function(x) sum(x&gt;0) ))#\u7ed8\u5236\u7bb1\u7ebf\u56fe<\/p>\n<p>\u5f97\u5230\u4ee5\u4e0a\u7684\u7bb1\u7ebf\u56fe\u540e\uff0c\u5c0f\u679c\u6765\u7ed9\u5927\u5bb6\u505a\u4e00\u4e2a\u7b80\u5355\u7684\u89e3\u8bfb\u5427\uff01\u7531\u7bb1\u7ebf\u56fe\u7684\u4e2d\u4f4d\u7ebf\u53ef\u4ee5\u770b\u51fa\uff0c\u57fa\u56e0\u8868\u8fbe\u6210\u529f\u7684\u7ec6\u80de\u6570\u7684\u4e2d\u4f4d\u6570\u503c\u5927\u6982\u57283\u4e2a\u5de6\u53f3\uff0c\u8868\u8fbe\u7ec6\u80de\u6570\u7684\u9608\u503c\u5927\u6982\u572835\u5de6\u53f3\uff0c\u5373\u8bf4\u660e\u5982\u679c\u57fa\u56e0\u572835\u4e2a\u7ec6\u80de\u5185\u4e0d\u8868\u8fbe\u5c31\u53ef\u4ee5\u76f4\u63a5\u8fc7\u6ee4\u6389\u54e6\u3002<\/p>\n<ul>\n<li>\n<ul>\n<li><strong>\u7edf\u8ba1\u6240\u6709\u7ec6\u80de\u8868\u8fbe\u4e86\u7684\u57fa\u56e0\u4e2a\u6570<\/strong><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>\u6709\u4e86\u4e0a\u9762\u7684\u57fa\u7840\uff0c\u4f60\u662f\u5426\u5b66\u4f1a\u4e3e\u4e00\u53cd\u4e09\u4e86\u5462\uff1f\u5bf9\u4e8e\u7ec6\u80de\u8868\u8fbe\u4e86\u7684\u57fa\u56e0\u4e2a\u6570\uff0c\u6211\u4eec\u540c\u6837\u4f7f\u7528apply\u51fd\u6570\u7edf\u8ba1\u6570\u636e\u77e9\u9635\u4e2d\u7684\u6bcf\u4e00\u5217\u5143\u7d20\u4e2d\u5bf9\u5e94\u503c\u5927\u4e8e0\u7684\u4e2a\u6570\u5373\u53ef\uff0c\u63a5\u4e0b\u6765\u6211\u4eec\u7528\u76f4\u65b9\u5206\u5e03\u56fe\u6765\u548c\u5927\u5bb6\u5c55\u793a\u4e00\u4e0b\u6574\u4f53\u7684\u8868\u8fbe\u60c5\u51b5\u3002\u548c\u5c0f\u679c\u4e00\u8d77\u770b\u770b\u5427\uff01<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"466\" class=\"wp-image-30040\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-3.png?resize=640%2C466\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-3.png?w=2460 2460w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-3.png?resize=300%2C219 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-3.png?resize=1024%2C746 1024w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-3.png?resize=768%2C559 768w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-3.png?resize=1536%2C1119 1536w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-3.png?resize=2048%2C1492 2048w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-3.png?resize=600%2C437 600w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-3.png?w=1280 1280w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/02\/word-image-30037-3.png?w=1920 1920w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/> fivenum(apply(counts,2,function(x) sum(x&gt;0) ))#\u7edf\u8ba1\u6240\u6709\u7ec6\u80de\u8868\u8fbe\u4e86\u57fa\u56e0\u4e2a\u6570\u4e2d\u7684\u4e94\u4e2a\u6307\u6807<br \/>\nhist(apply(counts,2,function(x) sum(x&gt;0) )) #\u7ed8\u5236\u76f4\u65b9\u5206\u5e03\u56fe<\/p>\n<p>\u5728\u5206\u6790\u4e86\u7bb1\u7ebf\u56fe\u7684\u7edf\u8ba1\u7ed3\u679c\u540e\uff0c\u4f60\u80fd\u5426\u72ec\u7acb\u5206\u6790\u76f4\u65b9\u56fe\u7684\u7edf\u8ba1\u7ed3\u679c\u5462\uff1f\u6211\u4eec\u8fd8\u662f\u548c\u5c0f\u679c\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\uff01<\/p>\n<p>\u7531\u76f4\u65b9\u5206\u5e03\u56fe\u7684\u7edf\u8ba1\u7ed3\u679c\u53ef\u4ee5\u770b\u51fa\uff0c\u8868\u8fbe\u57fa\u56e0\u4e2a\u6570\u57283000~4000\u4e4b\u95f4\u7684\u7ec6\u80de\u5360\u6700\u591a\u6570\u91cf\uff0c\u8868\u8fbe\u57fa\u56e0\u4e2a\u6570\u57288000\u4ee5\u4e0a\u7684\u7ec6\u80de\u4ec5\u67092~3\u4e2a\uff0c\u5360\u6700\u5c11\u7684\u6570\u91cf\u3002<\/p>\n<p>\u901a\u8fc7\u4ee5\u4e0a\u4e24\u7ec4\u7edf\u8ba1\u56fe\u7684\u5206\u6790\uff0c\u6211\u4eec\u5c31\u5bf9\u57fa\u56e0\u5728\u7ec6\u80de\u4e2d\u7684\u8868\u8fbe\u60c5\u51b5\u6e38\u5ba2\u6574\u4f53\u7684\u628a\u63e1\uff0c\u600e\u4e48\u6837\uff0c\u4eca\u5929\u7684\u5c0f\u6280\u5de7\u4f60\u5b66\u5e9f\u4e86\u561b\uff1f\u66f4\u591a\u751f\u4fe1\u6280\u5de7\u8bf7\u7ee7\u7eed\u5173\u6ce8\u5c0f\u679c\u54e6\uff01<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u548cR\u8bed\u8a00\u4e00\u8d77\u63a2\u7d22\u5355\u7ec6\u80de\u57fa\u56e0\u8868\u8fbe\uff01 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[&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/30037"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=30037"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/30037\/revisions"}],"predecessor-version":[{"id":30041,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/30037\/revisions\/30041"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=30037"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=30037"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=30037"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}