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\"\"<\/p>\n

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2.\u00a0\u5229\u7528\u52a0\u6743\u57fa\u56e0\u5171\u8868\u8fbe\u7f51\u7edc\u5206\u6790\u786e\u5b9a CSC \u7279\u5f81<\/strong><\/p>\n

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\u8868\u578b\u53ef\u5851\u6027\u6a21\u578b\u8868\u660e\uff0c\u764c\u7ec6\u80de\u5e72\u7ec6\u80de\u662f\u764c\u7ec6\u80de\u4e2d\u7684\u4e00\u4e2a\u52a8\u6001\u4e9a\u7fa4\uff0c\u800c\u4e0d\u662f\u4e00\u4e2a\u7a33\u5b9a\u7684\u7ec6\u80de\u7fa4\u3002\u8d8a\u6765\u8d8a\u591a\u7684\u62a5\u544a\u8fd8\u663e\u793a\uff0c\u67d0\u4e9b\u764c\u7ec6\u80de\u53ef\u4ee5\u901a\u8fc7\u5728 CSC \u548c\u975e CSC \u72b6\u6001\u4e4b\u95f4\u7684\u53ef\u9006\u8f6c\u6362\u8868\u73b0\u51fa\u53ef\u5851\u6027\uff0c\u4ece\u800c\u91cd\u65b0\u586b\u5145 CSC \u6c60\uff0c\u4f7f\u7ec6\u80de\u5728\u6cbb\u7597\u4e2d\u5b58\u6d3b\u4e0b\u6765\u3002\u5229\u7528 WGCNA \u5bf9 CSC \u548c\u975e CSC \u72b6\u6001\u4e4b\u95f4\u7684\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\u8fdb\u884c\u4e86\u7ec6\u81f4\u7684\u7814\u7a76\u3002\u6700\u521d\u7eb3\u5165\u4e86\u6765\u81ea 320 \u4e2a\u5143\u7ec6\u80de\u7684\u524d 5000 \u4e2a\u53ef\u53d8\u57fa\u56e0\u7684\u8868\u8fbe\u8c31\u3002\u4e3a\u4e86\u6784\u5efa\u65e0\u6807\u5ea6\u62d3\u6251\u6a21\u578b\uff0c\u5c06\u8f6f\u9608\u503c\u529f\u7387\u8bbe\u4e3a 9\uff08\u56fe 2c\uff09\u3002\u7ecf\u8fc7\u57fa\u4e8e\u6743\u91cd\u7684\u7b5b\u9009\uff0c\u5171\u5f97\u5230\u4e86 27 \u4e2a\u6a21\u5757\uff0c\u5176\u5206\u5c42\u805a\u7c7b\u6811\u5982\u56fe 2D \u6240\u793a\u3002\u5916\u663e\u5b50\u76f8\u90bb\u70ed\u56fe\u63cf\u8ff0\u4e86\u6240\u6709\u6a21\u5757\u95f4\u7684\u76f8\u5173\u6027\uff08\u56fe 2e\uff09\u3002\u7136\u540e\uff0c\u7814\u7a76\u4e86\u6bcf\u4e2a\u6a21\u5757\u4e0e\u4ee3\u8868\u5e72\u6027\u6c34\u5e73\u7684CytoTRACE\u5f97\u5206\u4e4b\u95f4\u7684\u76f8\u5173\u6027\u3002\u5728\u8fd9\u4e9b\u6a21\u5757\u4e2d\uff0cMEturquois\u4e0eCytoTRACE\u5f97\u5206\u7684\u76f8\u5173\u6027\u6700\u4e3a\u663e\u8457\uff08\u56fe2f\uff09\u3002\u7814\u7a76\u53d1\u73b0\uff0cMETurquoise \u6a21\u5757\u6240\u5305\u542b\u7684\u57fa\u56e0\u4e0e\u8bb8\u591a\u589e\u6b96\u76f8\u5173\u901a\u8def\u6709\u5173\u3002\u8fd9\u4e9b\u901a\u8def\u5305\u62ecG2M\u68c0\u67e5\u70b9\u3001MYC\u68c0\u67e5\u70b9\u548cE2F\u9776\u70b9\uff0c\u6240\u6709\u8fd9\u4e9b\u90fd\u4e0eCSCs\u7684\u7279\u5f81\u76f8\u5173\u3002\u968f\u540e\u6784\u5efa\u80bf\u7624\u7279\u5f02\u6027\u5e72\u6027\u7279\u5f81\uff0c\u8bc6\u522b\u4e86\u80bf\u7624\u7ec6\u80de\u4e0e\u5176\u4ed6\u7c7b\u578b\u57fa\u8d28\u7ec6\u80de\u548c\u514d\u75ab\u7ec6\u80de\u76f8\u6bd4\u7684\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\uff08DEGs\uff09\u3002\u901a\u8fc7\u5c06\u6076\u6027\u7ec6\u80de\u7684\u6807\u8bb0\u57fa\u56e0\u4e0eMEturquoise\u6a21\u5757\u4e2d\u7684\u57fa\u56e0\u4ea4\u53c9\uff0c\u53d1\u73b0\u4e86107\u4e2aCSC\u76f8\u5173\u57fa\u56e0\uff0c\u8fd9\u4e9b\u57fa\u56e0\u6784\u6210\u4e86CSC\u7279\u5f81\uff08\u56fe2g\uff09\u3002<\/p>\n

\"\u56fe2\"<\/p>\n

\u56fe2 \u5229\u7528WGCNA\u9274\u5b9aCSC\u7279\u5f81<\/p>\n

3.\u00a0CSC\u4e0e\u4e0d\u826f\u9884\u540e\u548c\u4e0d\u826f\u514d\u75ab\u6cbb\u7597\u53cd\u5e94\u76f8\u5173<\/strong><\/p>\n

\u968f\u540e\uff0c\u4e3a\u4e86\u68c0\u9a8c\u6240\u53d1\u73b0\u7684 CSC \u7279\u5f81\u7684\u53ef\u9760\u6027\uff0c\u4f7f\u7528 AUCell \u7b97\u6cd5\u8ba1\u7b97\u4e86\u6bcf\u4e2a\u6076\u6027\u7ec6\u80de\u7684 CSC \u5f97\u5206\u3002\u5728\u53d1\u73b0\u6570\u636e\u96c6\u4e2d\uff08GSE151530 \u548c GSE149614 \u7684\u7ec4\u5408\uff09\uff0cCSC \u8bc4\u5206\u4e0e CytoTRACE \u8bc4\u5206\u7684\u76f8\u5173\u6027\u8fbe\u5230\u4e86 0.80\uff0cP \u503c\u5c0f\u4e8e 0.0001\uff08\u56fe 3a\uff09\u3002\u4e3a\u4e86\u9a8c\u8bc1\u8fd9\u79cd\u76f8\u5173\u6027\uff0c\u53c8\u6536\u96c6\u4e86\u4e24\u4e2a HCC \u5355\u7ec6\u80de\u6570\u636e\u96c6\uff08GSE166635\u3001GSE146115\uff09\uff0c\u4e24\u4e2a\u6570\u636e\u96c6\u7684\u4e24\u4e2a\u8bc4\u5206\u4e4b\u95f4\u7684\u76f8\u5173\u6027\u5206\u522b\u4e3a 0.88 \u548c 0.81\uff08\u56fe 3a\uff09\u3002\u8fd9\u4e9b\u7814\u7a76\u4e3a 107 \u4e2a WGCNA \u9274\u5b9a\u57fa\u56e0\u7684\u6709\u6548\u6027\u53ca\u5176\u529f\u80fd\u76f8\u5173\u6027\u63d0\u4f9b\u4e86\u8bc1\u636e\u3002<\/p>\n

\u4e3a\u4e86\u63a2\u7d22\u764c\u7ec6\u80de\u5e72\u7ec6\u80de\u5728\u5927\u4f53\u6c34\u5e73\u4e0a\u7684\u4f5c\u7528\uff0c\u5206\u6790\u4e86TCGA\u961f\u5217\u4e2d\u7684\u6570\u636e\uff0c\u89c2\u5bdf\u5230\u4e0e\u90bb\u8fd1\u6b63\u5e38\u7ec4\u7ec7\u76f8\u6bd4\uff0c\u764c\u7ec6\u80de\u5e72\u7ec6\u80de\u5728\u80bf\u7624\u6837\u672c\u4e2d\u7684\u6d78\u6da6\u663e\u8457\u4e0a\u8c03\uff08\u56fe 3b\uff09\u3002\u6b64\u5916\uff0cCSC \u7684\u6d78\u6da6\u968f\u7740 HCC \u5206\u671f\u7684\u8fdb\u5c55\u800c\u589e\u52a0\uff08\u56fe 3c\uff09\u3002\u8fd8\u5bf9\u5185\u90e8 32 \u4f8b HCC \u60a3\u8005\u961f\u5217\u4e2d CSC \u9ad8\u8868\u8fbe\u7684\u4e94\u79cd\u86cb\u767d\uff08ENO1\u3001TKT\u3001CD63\u3001G6PD \u548c KRT8\uff09\u8fdb\u884c\u4e86\u514d\u75ab\u7ec4\u7ec7\u5316\u5b66\uff08IHC\uff09\u68c0\u6d4b\u3002\u4e0e\u8fd9\u4e9b\u7ed3\u679c\u4e00\u81f4\u7684\u662f\uff0cIHC \u67d3\u8272\u8bc1\u5b9e\u4e86\u5b83\u4eec\u4e0e\u8be5\u961f\u5217\u4e2d\u7684\u665a\u671f\u76f8\u5173\uff08\u56fe 3d \u548c e\uff09\u3002\u6b64\u5916\uff0cTCGA\u961f\u5217\u4e2dCSC\u6d78\u6da6\u7a0b\u5ea6\u8f83\u9ad8\u7684HCC\u60a3\u8005\u603b\u751f\u5b58\u671f\uff08OS\uff09\u8f83\u77ed\uff08\u56fe3f\uff09\u3002<\/p>\n

\u6b64\u5916\uff0c\u8fd8\u89c2\u5bdf\u5230\u4e0e CSC \u91cd\u5851\u76f8\u5173\u7684 TME \u6210\u5206\u53d1\u751f\u4e86\u91cd\u5927\u53d8\u5316\u3002\u5de8\u566c\u7ec6\u80deM2\u662f\u514d\u75ab\u6291\u5236\u6027\u9ad3\u7cfb\u7ec6\u80de\u7684\u4e00\u79cd\u4e9a\u578b\uff0c\u4e0eCSC\u5448\u6b63\u76f8\u5173\uff0c\u800cCD8+ T\u7ec6\u80de\u4e0eCSC\u5448\u8d1f\u76f8\u5173\uff0c\u8fd9\u8868\u660eCSC\u4e0e\u5de8\u566c\u7ec6\u80deM2\u4e4b\u95f4\u53ef\u80fd\u5b58\u5728\u76f8\u4e92\u4f5c\u7528\uff0c\u963b\u788d\u6297\u80bf\u7624\u7ec6\u80de\u7684\u6d78\u6da6\uff08\u56fe3g\uff09\u3002\u7eb3\u5165\u4e86\u6700\u8fd1\u4e00\u9879\u7814\u7a76 GSE238264 \u4e2d\u7684 ST \u6570\u636e\uff0c\u8be5\u7814\u7a76\u91cd\u70b9\u5173\u6ce8\u63a5\u53d7\u514d\u75ab\u7597\u6cd5\u6cbb\u7597\u7684 HCC \u60a3\u8005\u3002\u8be5\u6570\u636e\u96c6\u5305\u62ec\u6765\u81ea\u65b0\u8f85\u52a9\u5361\u535a\u66ff\u5c3c\uff08\u4e00\u79cd\u4e3b\u8981\u963b\u65ad\u8840\u7ba1\u5185\u76ae\u751f\u957f\u56e0\u5b50\u7684\u591a\u916a\u6c28\u9178\u6fc0\u9176\u6291\u5236\u5242\uff09\u548c\u5c3c\u4f10\u5355\u6297\uff08\u4e00\u79cd PD-1 \u6291\u5236\u5242\uff09\u4e34\u5e8a\u8bd5\u9a8c\u7684\u6837\u672c\uff0c\u5e76\u6839\u636e\u6cbb\u7597\u7ed3\u679c\u533a\u5206\u4e86\u5e94\u7b54\u8005\u548c\u975e\u5e94\u7b54\u8005\u3002\u4e0e\u5e94\u7b54\u8005\u76f8\u6bd4\uff0c\u975e\u5e94\u7b54\u8005\u7684 CSC \u5f97\u5206\u66f4\u9ad8\uff0c\u5f3a\u8c03\u4e86 CSC \u5728\u514d\u75ab\u6cbb\u7597\u5e94\u7b54\u4e2d\u7684\u6f5c\u5728\u76f8\u5173\u6027\uff08\u56fe 3h\uff09\u3002GSEA\u663e\u793a\uff0c\u5728\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\uff08NSCLC\uff09\u3001\u76ae\u80a4\u9ed1\u8272\u7d20\u7624\uff08SKCM\uff09\u3001\u80be\u7ec6\u80de\u764c\uff08RCC\uff09\u7b49\u4e03\u79cd\u514d\u75ab\u7597\u6cd5\u961f\u5217\u4e2d\uff0c\u975e\u5e94\u7b54\u8005\u7684 CSC \u5747\u6709\u5bcc\u96c6\uff08\u56fe 3i\uff09\u3002\u8fd9\u4e00\u7ed3\u679c\u4e3a CSC \u5728\u514d\u75ab\u6cbb\u7597\u4e2d\u7684\u4e0d\u5229\u4f5c\u7528\u63d0\u4f9b\u4e86\u66f4\u591a\u8bc1\u636e\u3002<\/p>\n

\"\u56fe3\"<\/p>\n

\u56fe3 CSC\u4e0e\u8f83\u5dee\u9884\u540e\u548c\u4e0d\u826f\u514d\u75ab\u7597\u6cd5\u53cd\u5e94\u7684\u76f8\u5173\u6027<\/p>\n

4.\u00a0\u9ad8CSC\u7ec4\u4e2dMacro_SPP1\u7684\u79ef\u7d2f<\/strong><\/p>\n

\u63a5\u7740\u7814\u7a76\u4e86CSC\u5bf9TME\u7684\u5f71\u54cd\u3002\u6bcf\u4e2a\u6837\u672c\u7684CSC\u5f97\u5206\u88ab\u5b9a\u4e49\u4e3a\u6837\u672c\u4e2d\u6240\u6709\u6076\u6027\u7ec6\u80de\u7684\u5e73\u5747CSC\u5f97\u5206\u3002\u6839\u636e\u6240\u6709\u6837\u672c\u7684CSC\u5f97\u5206\u4e2d\u4f4d\u6570\uff0c\u5c0620\u4e2a\u6837\u672c\u5206\u4e3aCSC\u9ad8\u5206\u7ec4\u548cCSC\u4f4e\u5206\u7ec4\u3002\u8fd9\u4e24\u7ec4\u6837\u672c\u7684TME\u6210\u5206\u5448\u73b0\u51fa\u4e0d\u540c\u7684\u666f\u89c2\uff08\u56fe4a\uff09\u3002\u4e0e CSC \u4f4e\u5206\u7ec4\u76f8\u6bd4\uff0cCSC \u9ad8\u5206\u7ec4\u7684\u6076\u6027\u7ec6\u80de\u6bd4\u4f8b\u660e\u663e\u66f4\u9ad8\u3002\u89c2\u5bdf\u5230 CSC \u9ad8\u5206\u7ec4\u4e0e CSC \u4f4e\u5206\u7ec4\u76f8\u6bd4\uff0c\u9ad3\u7cfb\u7ec6\u80de\u6d78\u6da6\u7a0b\u5ea6\u66f4\u9ad8\uff0c\u800c CSC \u4f4e\u5206\u7ec4\u4e0e CSC \u9ad8\u5206\u7ec4\u76f8\u6bd4\uff0cT \u7ec6\u80de\u6d78\u6da6\u7a0b\u5ea6\u66f4\u9ad8\u3002\u4e0d\u8fc7\uff0cB \u7ec6\u80de\u3001\u5185\u76ae\u7ec6\u80de\u548c\u6210\u7ea4\u7ef4\u7ec6\u80de\u7684\u6bd4\u4f8b\u5728\u4e24\u7ec4\u4e4b\u95f4\u6ca1\u6709\u660e\u663e\u5dee\u5f02\u3002<\/p>\n

\u9ad3\u7cfb\u7ec6\u80de\u5728 HCC \u60a3\u8005\u4e2d\u7684\u6d78\u6da6\u589e\u5f3a\uff0c\u8868\u660e\u5b83\u4eec\u5728\u4fc3\u8fdb CSC \u542f\u52a8\u65b9\u9762\u53d1\u6325\u7740\u529f\u80fd\u6027\u4f5c\u7528\u3002\u968f\u540e\uff0c\u91cd\u70b9\u7814\u7a76\u4e86\u4e24\u7ec4\u60a3\u8005\u9ad3\u7cfb\u7ec6\u80de\u4e9a\u578b\u7684\u53d8\u5316\u3002\u9996\u5148\uff0c\u5c06\u9ad3\u7cfb\u7ec6\u80de\u5206\u4e3a\u516d\u4e2a\u4e9a\u7fa4\uff0c\u5305\u62ecDC\u3001\u5355\u6838\u7ec6\u80de\u3001SPP1+\u5de8\u566c\u7ec6\u80de\uff08Macro_SPP1\uff09\u3001MRC1+\u5de8\u566c\u7ec6\u80de\uff08Macro_MRC1\uff09\u3001\u6210\u8102\u6027\u5de8\u566c\u7ec6\u80de\uff08Macro_adipo\uff09\u548c\u589e\u6b96\u6027\u5de8\u566c\u7ec6\u80de\uff08Macro_prolif\uff09\u3002\u7ed3\u679c\u663e\u793a\uff0cMacro_SPP1 \u4e9a\u7fa4\u4e3b\u8981\u51fa\u73b0\u5728 CSC \u9ad8\u5206\u7ec4\u4e2d\uff08\u56fe 4b\uff09\u3002\u636e\u62a5\u9053\uff0cMacro_SPP1 \u662f\u5de8\u566c\u7ec6\u80de M229 \u7684\u4e00\u79cd\u4e9a\u578b\u3002Macro_SPP1\u4ee3\u8868\u4e86CSC\u7279\u5f02\u6027\u5de8\u566c\u7ec6\u80de\uff0c\u5360CSC\u9ad8\u5206\u7ec4\u6837\u672c\u9ad3\u7cfb\u7ec6\u80de\u768423%\uff0c\u800c\u5728CSC\u4f4e\u5206\u7ec4\u4e2d\u4ec5\u5360\u9ad3\u7cfb\u7ec6\u80de\u76841.8%\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u8868\u8fbe\u8102\u8d28\u4ee3\u8c22\u76f8\u5173\u57fa\u56e0\uff08ALB\u3001APOA2\u3001FABP1\u548cAPOH\uff09\u7684\u6210\u8102\u6027\u9ad3\u6837\u7ec6\u80de\u4e9a\u7fa4Macro_adipo\u5728CSC\u4f4e\u5206\u7ec4\u4e2d\u7684\u6d78\u6da6\u7a0b\u5ea6\u66f4\u9ad8\u3002<\/p>\n

5.\u00a0Macro_SPP1\u8868\u8fbe\u91d1\u5c5e\u86cb\u767d\u9176\u5e76\u53d7HIF1A\u8c03\u63a7<\/strong><\/p>\n

\u4e0e\u5176\u4ed6\u9ad3\u7cfb\u4e9a\u578b\u76f8\u6bd4\uff0cMacro_SPP1 \u5177\u6709\u72ec\u7279\u7684\u529f\u80fd\u3002Macro_SPP1 \u8868\u73b0\u51fa\u8f83\u9ad8\u7684\u91d1\u5c5e\u86cb\u767d\u9176\u8868\u8fbe\u6c34\u5e73\uff0c\u5982 MMP9\u3001MMP12 \u548c MMP7\uff0c\u5b83\u4eec\u53c2\u4e0e\u7ec6\u80de\u5916\u57fa\u8d28\u91cd\u5851\u548c\u514d\u75ab\u53cd\u5e94\u8c03\u828230\uff08\u56fe 4c\uff09\u3002\u6b64\u5916\uff0cMacro_SPP1 \u8fd8\u8868\u8fbe CXCL3\u3001CCL20\u3001CCL7 \u548c CXCL8 \u7b49\u8d8b\u5316\u56e0\u5b50\u3002\u5728 Macro_SPP1 \u4e2d\u9ad8\u8868\u8fbe\u7684 DEGs \u5bcc\u96c6\u4e8e\u7f3a\u6c27\u3001ECM-\u53d7\u4f53\u76f8\u4e92\u4f5c\u7528\u3001\u4e0a\u76ae-\u95f4\u8d28\u8f6c\u5316\uff08EMT\uff09\u3001\u91d1\u5c5e\u80bd\u9176\u6d3b\u6027\u3001KRAS \u4fe1\u53f7\u4f20\u5bfc\u548c\u7cd6\u9175\u89e3\uff08\u56fe 4d\uff09\u3002\u7136\u800c\uff0c\u514d\u75ab\u76f8\u5173\u9014\u5f84\uff0c\u5305\u62ec\u6297\u539f\u5904\u7406\u548c\u9012\u5448\u3001\u6dcb\u5df4\u7ec6\u80de\u4ecb\u5bfc\u7684\u514d\u75ab\u548c\u8865\u4f53\u6fc0\u6d3b\uff0c\u5728 Macro_SPP1 \u4e2d\u53d7\u5230\u663e\u8457\u6291\u5236\u3002\u8fd9\u4e9b\u53d1\u73b0\u8868\u660e\uff0cMacro_SPP1 \u53ef\u80fd\u5728\u4fc3\u8fdb EMT \u548c\u6291\u5236 HCC \u7684\u514d\u75ab\u53cd\u5e94\u4e2d\u53d1\u6325\u4f5c\u7528\u3002<\/p>\n

\u6b64\u5916\uff0cMacro_SPP1 \u7684\u6d3b\u6027\u53ef\u80fd\u53d7\u8f6c\u5f55\u56e0\u5b50 HIF1A \u7684\u8c03\u63a7\uff0cHIF1A \u662f\u7f3a\u6c27\u8bf1\u5bfc\u901a\u8def\u4e2d\u7684\u4e00\u4e2a\u5173\u952e\u56e0\u5b50\uff08\u56fe 4e\uff09\u3002\u53e6\u4e00\u4e2a\u8f6c\u5f55\u56e0\u5b50FOSL1\u4e5f\u7531Macro_SPP1\u8868\u8fbe\uff0c\u5df2\u77e5\u5b83\u80fd\u8c03\u8282\u4fb5\u88ad\u548c\u8f6c\u79fb\uff0c\u4e0eEMT\u548c\u764c\u7ec6\u80de\u5e72\u6027\u6709\u5173\u3002HSF1 \u80fd\u591f\u4fdd\u62a4\u7ec6\u80de\u514d\u53d7\u538b\u529b\uff0c\u907f\u514d\u86cb\u767d\u8d28\u7ec4\u4e0d\u7a33\u5b9a\uff0c\u6291\u5236\u80bf\u7624\u6291\u5236\u6027\u6dc0\u7c89\u6837\u86cb\u767d\u751f\u6210\u3002\u8fd9\u4e9b\u7814\u7a76\u7ed3\u679c\u8868\u660e\uff0c\u8fd9\u4e9b\u8f6c\u5f55\u56e0\u5b50\u7684\u6fc0\u6d3b\u53c2\u4e0e\u4e86Macro_SPP1\u7684\u627f\u8bfa\u3002\u9274\u4e8e CSC \u548c Macro_SPP1 \u90fd\u4e0e\u7f3a\u6c27\u8bf1\u5bfc\u9014\u5f84\u6709\u5173\uff0c\u63a8\u6d4b\u5728\u80bf\u7624\u7684\u7f3a\u6c27\u533a\u57df\u5185\u5b58\u5728\u4e00\u4e2a\u8fde\u63a5 Macro_SPP1 \u548c CSC \u7684\u5c40\u90e8\u7f51\u7edc\uff0c\u5171\u540c\u52a0\u5267\u4e86 HCC \u7684\u5fae\u73af\u5883\u3002<\/p>\n

\u4e3a\u4e86\u9a8c\u8bc1Macro_SPP1\u6d78\u6da6\u7684\u91cd\u8981\u6027\uff0c\u5206\u6790\u4e86\u6765\u81eaTCGA\u961f\u5217\u7684\u6570\u636e\uff0c\u89c2\u5bdf\u5230\u4e0e\u90bb\u8fd1\u6b63\u5e38\u7ec4\u7ec7\u76f8\u6bd4\uff0c\u80bf\u7624\u6837\u672c\u4e2dMacro_SPP1\u7684\u6d78\u6da6\u663e\u8457\u4e0a\u8c03\uff08\u56fe4f\uff09\u3002Macro_SPP1 \u7684\u6d78\u6da6\u968f\u7740 HCC \u75c5\u671f\u7684\u5ef6\u957f\u800c\u589e\u52a0\uff08\u56fe 4g\uff09\u3002\u6b64\u5916\uff0cTCGA\u961f\u5217\u4e2dMacro_SPP1\u6d78\u6da6\u7a0b\u5ea6\u8f83\u9ad8\u7684HCC\u60a3\u8005\u7684OS\u8f83\u77ed\uff08\u56fe4h\uff09\u3002\u514d\u75ab\u6cbb\u7597\u961f\u5217\u4e2d\u7684\u975e\u5e94\u7b54\u8005\u4e0e\u5e94\u7b54\u8005\u76f8\u6bd4\u8868\u73b0\u51fa\u66f4\u9ad8\u7684 Macro_SPP1\uff0c\u5f3a\u8c03\u4e86 Macro_SPP1 \u5728\u514d\u75ab\u6cbb\u7597\u5e94\u7b54\u4e2d\u7684\u6f5c\u5728\u76f8\u5173\u6027\uff08\u56fe 4i\uff09\u3002<\/p>\n

\"\u56fe4\"<\/p>\n

\u56fe4 Macro_SPP1\u8868\u8fbe\u91d1\u5c5e\u86cb\u767d\u9176\uff0c\u5e76\u53d7 HIF1A \u8c03\u8282<\/p>\n

6.\u00a0\u901a\u8fc7\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\u63ed\u793a CSC \u548c Macro_SPP1 \u7684\u5171\u5b9a\u4f4d<\/strong><\/p>\n

\u4e3a\u4e86\u7814\u7a76 CSC \u548c Macro_SPP1 \u7684\u7a7a\u95f4\u7ec4\u7ec7\uff0c\u4f7f\u7528 8 \u4f8b HCC \u60a3\u8005\u7684\u80bf\u7624\u7ec4\u7ec7\u5207\u7247\u8fdb\u884c\u4e86\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\u6d4b\u5e8f\u3002\u83b7\u5f97\u4e86\u6240\u6709\u6837\u672c\u4e2d\u4f4d\u6570\u4e3a 4900 \u4e2a\u70b9\u7684\u8f6c\u5f55\u7ec4\u5b66\u6570\u636e\u3002\u4e0e\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u7684\u590d\u6742\u6027\u7c7b\u4f3c\uff0c\u4ec5\u6839\u636e ST \u6570\u636e\u4e2d\u7684\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\u8bc6\u522b\u6076\u6027\u7ec6\u80de\u4e5f\u5177\u6709\u6311\u6218\u6027\uff0c\u5c24\u5176\u662f\u5728\u533a\u5206\u6076\u6027\u7ec6\u80de\u548c\u6b63\u5e38\u4e0a\u76ae\u7ec6\u80de\u65f6\u3002\u4e3a\u4e86\u89e3\u51b3\u8fd9\u4e00\u590d\u6742\u95ee\u9898\uff0c\u8fdb\u884c\u4e86\u63a8\u65ad CNV \u5206\u6790\uff0c\u6839\u636e CNV \u6a21\u5f0f\u4ece\u5176\u4ed6\u7ec6\u80de\u7c7b\u578b\u4e2d\u7279\u5f02\u6027\u5730\u5212\u5206\u51fa\u6076\u6027\u7ec6\u80de\u3002<\/p>\n

\u63a8\u65adCNV\u5206\u6790\u7684\u8fc7\u7a0b\u5305\u62ec\u4e24\u4e2a\u805a\u7c7b\u9636\u6bb5\u3002\u9996\u5148\uff0c\u5728\u7b2c\u4e00\u4e2a\u805a\u7c7b\u9636\u6bb5\uff0c\u5229\u7528 Seurat \u8f6f\u4ef6\u5305\u5bf9 ST \u6570\u636e\u8fdb\u884c\u964d\u7ef4\u548c\u805a\u7c7b\u3002\u8fd0\u884c PCA \u964d\u7ef4\u540e\uff0c\u4f7f\u7528 Seurat \u8f6f\u4ef6\u5305\u4e2d FindClusters \u529f\u80fd\u4e2d\u7684 KNN \u7b97\u6cd5\u5bf9\u7a7a\u95f4\u70b9\u8fdb\u884c\u805a\u7c7b\u3002\u6837\u672c 1 \u4e2d\u7684 4825 \u4e2a\u70b9\u88ab\u5206\u6210 14 \u4e2a\u4e0d\u540c\u7684\u805a\u7c7b\uff08\u56fe 5a\uff09\u3002\u6839\u636e\u4e00\u7cfb\u5217\u514d\u75ab\u76f8\u5173\u7279\u5f81\uff0c\u5305\u62ec\u6cdb\u514d\u75ab\u6807\u8bb0\uff08PTPRC\uff09\u3001\u6cdb T \u7ec6\u80de\u6807\u8bb0\uff08CD2\u3001CD3D\u3001CD3E\u3001CD3G\uff09\u3001B \u7ec6\u80de\u6807\u8bb0\uff08CD79A\u3001MS4A1\u3001CD79B\uff09\u548c\u9ad3\u6837\u7ec6\u80de\u6807\u8bb0\uff08CD68\u3001CD14\uff09\uff0c\u8ba1\u7b97\u51fa\u6bcf\u4e2a\u70b9\u7684 “\u6b63\u5e38\u5f97\u5206”\uff0c\u4ee3\u8868\u6bcf\u4e2a\u70b9\u5185\u514d\u75ab\u7279\u5f81\u7684\u5e73\u5747\u503c\u3002\u5728\u7b2c\u4e00\u6b21\u805a\u7c7b\u5206\u6790\u4e2d\uff0c\u6b63\u5e38\u5f97\u5206\u6700\u9ad8\u7684\u805a\u7c7b 8 \u88ab\u6307\u5b9a\u4e3a\u63a8\u65ad CNV \u5206\u6790\u7684\u53c2\u8003\u805a\u7c7b\uff08\u56fe 5b\uff09\u3002<\/p>\n

\u7136\u540e\uff0c\u5728\u7b2c\u4e8c\u805a\u7c7b\u9636\u6bb5\uff0c\u57fa\u4e8e\u6811\u5212\u5206\u7684\u5206\u5c42\u805a\u7c7b\u65b9\u6cd5\u6839\u636e CNV \u6a21\u5f0f\u5c06\u9664\u53c2\u7167\u805a\u7c7b\u5916\u7684\u6240\u6709\u6591\u70b9\u5212\u5206\u4e3a\u516b\u4e2a\u805a\u7c7b\uff08\u56fe 5c\uff09\u3002CNV\u5f97\u5206\u8f83\u9ad8\u7684\u7c071\u3001\u7c072\u548c\u7c073\u88ab\u786e\u5b9a\u4e3a\u6076\u6027\u7c07\uff08\u56fe5d\uff09\u3002\u4e0e CNV \u5206\u6790\u7ed3\u679c\u4e00\u81f4\uff0c\u7c07 1\u3001\u7c07 2 \u548c\u7c07 3 \u4e0e\u5206\u6563\u7684\u80bf\u7624\u533a\u57df\u76f8\u5bf9\u5e94\uff0c\u800c\u5176\u4ed6\u7c07\u4e3b\u8981\u7531\u6b63\u5e38\u809d\u7ec6\u80de\u3001\u6210\u7ea4\u7ef4\u7ec6\u80de\u548c\u6df7\u5408\u514d\u75ab\u7ec6\u80de\u7ec4\u6210\uff08\u56fe 5e\uff09\u3002<\/p>\n

\u4e3a\u4e86\u8bc4\u4f30 CSC \u548c Macro_SPP1 \u7684\u5b58\u5728\uff0c\u4f7f\u7528\u5355\u7ec6\u80de\u5206\u6790\u5f97\u51fa\u7684\u7279\u5f81\u5bf9\u8be5\u6837\u672c\u4e2d\u7684\u6591\u70b9\u8fdb\u884c\u4e86\u8bc4\u5206\u3002\u89c2\u5bdf\u5230\u6837\u672c\u4e2d\u5b58\u5728\u5927\u91cf\u7684 CSC \u5f02\u8d28\u6027\uff0c\u8fd9\u5728\u5f88\u5927\u7a0b\u5ea6\u4e0a\u53d7\u5230\u5176\u7a7a\u95f4\u6a21\u5f0f\u7684\u5f71\u54cd\u3002CSC \u7279\u5f81\u5f97\u5206\u4e3b\u8981\u5728\u80bf\u7624\u7c07\u4e2d\u68c0\u6d4b\u5230\uff08\u56fe 5f\uff09\u3002\u4e09\u4e2a\u80bf\u7624\u96c6\u7fa4\u8868\u73b0\u51fa\u660e\u663e\u7684 CSC \u7279\u5f81\uff0c\u5176\u7c073 \u7684 CSC \u8bc4\u5206\u6700\u9ad8\uff08\u56fe 5f\uff09\u3002CD24\u548cCD44\u662f\u4f17\u6240\u5468\u77e5\u7684CSC\u6807\u8bb0\u7269\uff0c\u5728\u7c073\u4e2d\u9ad8\u8868\u8fbe\uff08\u56fe5g\uff09\u3002\u89c2\u5bdf\u5230Macro_SPP1 \u5728\u7c073 \u4e2d\u9760\u8fd1 CSC\uff08\u56fe 5h\uff09\u3002\u6b64\u5916\uff0cMacro_SPP1\u8fd8\u805a\u96c6\u5728\u7c073\u7684\u80bf\u7624\u8fb9\u754c\u9644\u8fd1\uff0c\u6784\u6210\u4e86\u7ef4\u6301CSC\u7684\u514d\u75ab\u6291\u5236\u524d\u6cbf\u3002\u7c073 \u7684 CSC \u548c Macro_SPP1 \u6c34\u5e73\u6700\u9ad8\uff08\u56fe 5i\uff09\u3002\u8fd9\u8868\u660e CSC \u548c Macro_SPP1 \u5728\u9f9b\u5185\u5b58\u5728\u7269\u7406\u76f8\u4e92\u4f5c\u7528\u3002<\/p>\n

\"\u56fe5\"<\/p>\n

\u56fe5 \u80bf\u7624\u533a\u57dfCSC\u548cMacro_SPP1\u7684\u5171\u5b9a\u4f4d<\/p>\n

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\"\u56fe6\"<\/p>\n

\u56fe6 HIF1A \u548c HAVCR2 \u5728\u5171\u5b9a\u4f4d\u533a\u8fb9\u754c\u9ad8\u8868\u8fbe<\/p>\n

8.\u00a0\u7ec6\u80de-\u7ec6\u80de\u901a\u8baf\u4e2d\u7684 SPP1 \u4fe1\u53f7\u4f20\u5bfc<\/strong><\/p>\n

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\u63a5\u4e0b\u6765\u91cd\u70b9\u8f6c\u79fb\u5230\u4e86\u8fb9\u754c\u533a\u57df\u7684\u901a\u8baf\u4e0a\uff0c\u5176\u4e2d\u7c07 3 \u662f\u6700\u6d3b\u8dc3\u7684\u7c07\uff0c\u53c2\u4e0e\u4e86\u8bb8\u591a\u4f20\u5165\u548c\u4f20\u51fa\u7684\u76f8\u4e92\u4f5c\u7528\uff08\u56fe 7b\uff09\u3002\u56e0\u6b64\u5c06\u6ce8\u610f\u529b\u96c6\u4e2d\u5728\u7c07 3 \u4e0e\u6240\u6709\u7c07\u4e4b\u95f4\u7684\u7ec6\u80de\u95f4\u901a\u8baf\u4e0a\u3002\u7c07 3 \u6fc0\u6d3b\u4e86\u5404\u79cd\u4fe1\u53f7\u901a\u8def\uff0c\u5305\u62ec SPP1\u3001MIF\u3001COMPLEMENT\u3001MK \u548c AGT\uff08\u56fe 7c\uff09\u3002\u5728\u6240\u6709\u901a\u8baf\u6d3b\u52a8\u4e2d\uff0cSPP1 \u4fe1\u53f7\u901a\u8def\u7684\u76f8\u5bf9\u5f3a\u5ea6\u6700\u9ad8\u3002\u7c07 3 \u662f SPP1 \u4fe1\u53f7\u901a\u8def\u7684\u4e3b\u8981\u53d1\u9001\u8005\uff0c\u800c\u7c07 3\u30014\u30015\u30016\u30017 \u548c\u8fb9\u754c\u533a\u57df\u5185\u7684\u53c2\u7167\u7fa4\u7c07\u5219\u662f SPP1 \u4fe1\u53f7\u7684\u63a5\u6536\u8005\u548c\u5f71\u54cd\u8005\uff08\u56fe 7d\uff09\u3002\u8fd9\u4e9b\u53d1\u73b0\u4e0e\u5355\u7ec6\u80de\u6c34\u5e73\u7ed3\u679c\u4e00\u81f4\uff0c\u8bc1\u660e\u4e86 SPP1 \u4e0e CSC \u4e0a\u7684 CD44\u3001ITGAV\u3001ITGA5\u3001ITGB1 \u548c ITGB5 \u76f8\u4e92\u4f5c\u7528\uff08\u56fe 7e\uff09\u3002\u603b\u4e4b\uff0c\u8fd9\u4e9b\u7ed3\u679c\u51f8\u663e\u4e86 SPP1 \u4fe1\u53f7\u5728\u4ecb\u5bfc Macro_SPP1 \u4e0e CSC \u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u4e2d\u7684\u91cd\u8981\u4f5c\u7528\uff0c\u4ee5\u53ca\u5b83\u4eec\u5728\u8fb9\u754c\u533a\u57df\u5171\u5b9a\u4f4d\u6240\u4ea7\u751f\u7684\u5f71\u54cd\u3002<\/p>\n

\"\u56fe7\"<\/p>\n

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\u6587\u7ae0\u5c0f\u7ed3<\/strong><\/p>\n

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