{"id":38069,"date":"2024-04-28T11:04:32","date_gmt":"2024-04-28T03:04:32","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=38069"},"modified":"2024-04-28T11:04:35","modified_gmt":"2024-04-28T03:04:35","slug":"%e5%9b%bd%e8%87%aa%e7%84%b6%e6%96%b0%e8%b4%b5cns%e9%a1%b6%e5%88%8a%e5%ae%a0%e5%84%bf-%e7%9b%b8%e5%88%86%e7%a6%bb%e4%b9%9f%e8%83%bd%e5%81%9a%e7%94%9f%e4%bf%a1","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=38069","title":{"rendered":"\u56fd\u81ea\u7136\u65b0\u8d35+CNS\u9876\u520a\u5ba0\u513f\u2014 \u2014\u201c\u76f8\u5206\u79bb\u201d\u4e5f\u80fd\u505a\u751f\u4fe1\uff1f\u770b\u8fd9\u91cc\uff0c\u80bf\u7624\u9884\u540e+\u5206\u578b\u5206\u6790\uff0c6\u5206+\u5e72\u6e7f\u7ed3\u5408\u601d\u8def\u7ed9\u4f60\u6253\u4e2a\u6837\uff01"},"content":{"rendered":"<p><img decoding=\"async\" loading=\"lazy\" class=\"alignnone  wp-image-36510\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/1.jpg?resize=640%2C137\" alt=\"\" width=\"640\" height=\"137\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/1.jpg?resize=300%2C64 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/1.jpg?resize=768%2C163 768w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/1.jpg?resize=600%2C128 600w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/1.jpg?w=940 940w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/><\/p>\n<p>\u5e38\u770b\u5c0f\u4e91\u516c\u4f17\u53f7\u7684\u7c89\u4e1d\u670b\u53cb\u4eec\uff0c\u5e94\u8be5\u6709\u53d1\u73b0\u5c0f\u4e91\u4f1a\u7ecf\u5e38\u5206\u4eab\u4e00\u4e9b\u56fd\u81ea\u7136\u70ed\u70b9\uff0c\u6bd4\u5982\u80bf\u7624\u514d\u75ab\u3001\u7ec6\u80de\u6b7b\u4ea1\u3001\u80bf\u7624\u4ee3\u8c22\u3001\u8868\u89c2\u9057\u4f20\u4fee\u9970\u7b49\u7b49~ ~<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"552\" height=\"399\" class=\"wp-image-38072\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-1.png?resize=552%2C399\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-1.png?w=552 552w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-1.png?resize=300%2C217 300w\" sizes=\"(max-width: 552px) 100vw, 552px\" data-recalc-dims=\"1\" \/><\/p>\n<p>\uff082022\u5e74\u56fd\u81ea\u7136\u70ed\u70b9\u65b9\u5411\uff0c\u56fe\u7247\u6765\u6e90\u4e8e\u7f51\u7edc\uff09<\/p>\n<p>\u4f46\u8fd9\u4e48\u591a\u56fd\u81ea\u7136\u70ed\u70b9\u4e2d\u6709\u4e00\u4e2a\u542c\u8d77\u6765\u597d\u50cf\u8ddf\u751f\u4fe1\u5b8c\u5168\u4e0d\u6cbe\u8fb9\u7684\u65b9\u5411\u5c0f\u4e91\u8fd8\u6ca1\u6709\u63a8\u8350\u8fc7\uff0c\u8fd8\u7ecf\u5e38\u51fa\u73b0\u5728CNS\u9876\u520a\u4e2d\uff0c\u5b83\u5c31\u662f\u201c\u76f8\u5206\u79bb\u201d\uff0c\u90a3\u5b83\u600e\u4e48\u62ff\u6765\u505a\u751f\u4fe1\u5462\uff1f\u5728\u770b\u601d\u8def\u4e4b\u524d\uff0c\u8fd8\u662f\u5148\u8ddf\u5c0f\u4e91\u521d\u6b65\u4e86\u89e3\u4e0b\u4ec0\u4e48\u662f\u76f8\u5206\u79bb\uff1f<strong>\uff08ps\uff1a\u8ffd\u65b0\u70ed\u70b9\u53ea\u8981\u8ddf\u7d27\u5c0f\u4e91\u5c31\u53ef\u4ee5\u5566\uff0c\u5c0f\u4e91\u53d1\u73b0\u65b0\u65b9\u5411\u4f1a\u7b2c\u4e00\u65f6\u95f4\u5206\u4eab\u7ed9\u7c89\u4e1d\u670b\u53cb\u4eec\u7684\uff09<\/strong><\/p>\n<p>\u76f8\u5206\u79bb (phase separation)\u662f\u5c06\u751f\u7269\u5206\u5b50\u4ece\u5747\u5300\u73af\u5883\u4e2d\u5206\u79bb\u6210\u4e24\u79cd\u4e0d\u540c\u7684\u76f8(\u51dd\u805a\u76f8\u548c\u7a00\u76f8)\u7684\u5bc6\u5ea6\u4f9d\u8d56\u6027\u5206\u79bb\u3002\u751f\u6d3b\u4e2d\u53ef\u4ee5\u89c1\u5230\u6c34\u4e0a\u6f02\u6d6e\u7684\u6cb9\u6ef4\uff0c\u5c31\u662f\u4e00\u79cd\u76f8\u5206\u79bb\u73b0\u8c61\u3002\u4e00\u5171\u4e24\u79cd\u76f8\uff0c\u5373\u6c34\u548c\u6cb9\uff0c\u7531\u4e8e\u90fd\u662f\u6db2\u4f53\uff0c\u4e5f\u53eb\u6db2\u6db2\u76f8\u5206\u79bb\uff08LLPS\uff0cliquid-liquid phase separation\uff09\u3002<\/p>\n<p>Hyman\u548cBrangwynne 2009\u5e74\u5728Science\u53d1\u6587\u7ae0\uff0c\u63d0\u51fa\u4e86\u7ec6\u80de\u5185\u901a\u8fc7\u201c\u76f8\u5206\u79bb\u201d\uff0c\u53ef\u4ee5\u63d0\u4f9b\u4e00\u79cd\u7279\u5b9a\u7684\u65b9\u5f0f\u8ba9\u7ec6\u80de\u5185\u7684\u7279\u5b9a\u5206\u5b50\u805a\u96c6\u8d77\u6765\uff0c\u4ece\u800c\u5728\u201c\u6df7\u4e71\u7684\u201d\u7ec6\u80de\u5185\u90e8\u5f62\u6210\u4e00\u5b9a\u201c\u79e9\u5e8f\u201d\u3002\u76f8\u5206\u79bb\u53c2\u4e0e\u65e0\u819c\u5668\u5b98\u7684\u5f62\u6210\u3001\u4fe1\u53f7\u8f6c\u5bfc\u3001\u7ec6\u80de\u9aa8\u67b6\u3001\u8d85\u5206\u5b50\u7ec4\u88c5\u3001\u57fa\u56e0\u7684\u6fc0\u6d3b\u3002\u76f8\u5206\u79bb\u5f02\u5e38\u53ef\u80fd\u5bfc\u81f4\u75be\u75c5\u53d1\u751f\uff0c\u6bd4\u5982\u795e\u7ecf\u9000\u884c\u6027\u75be\u75c5\u3001\u80bf\u7624\u3001\u8870\u8001\u7b49\u3002\u5f02\u5e38LLPS\u4e0e\u764c\u75c7\u7684\u51e0\u4e2a\u7279\u5f81\u5e7f\u6cdb\u76f8\u5173\uff0c\u5305\u62ec\u6301\u7eed\u589e\u6b96\u4fe1\u53f7\u3001\u751f\u957f\u6291\u5236\u9003\u907f\u3001\u7ec6\u80de\u6b7b\u4ea1\u62b5\u6297\u3001\u7aef\u7c92\u7ef4\u6301\u548cDNA\u635f\u4f24\u4fee\u590d\u3002<\/p>\n<p>\u5927\u6982\u4e86\u89e3\u4e86\u201c\u76f8\u5206\u79bb\u201d\u4e0e\u75be\u75c5\u7684\u5173\u7cfb\uff0c<strong>\u90a3\u5b83\u53c8\u8be5\u600e\u4e48\u5207\u5165\u505a\u751f\u4fe1\u5462\uff1f\u5176\u5b9e\u975e\u5e38\u7b80\u5355\uff0c\u5728\u5e38\u89c4\u5957\u8def\u4e0a\uff0c\u53ea\u9700\u8981\u6362\u4e2a\u201c\u76f8\u5206\u79bb\u57fa\u56e0\u96c6\u201d\u5c31\u53ef\u4ee5\u5566\uff0c\u8fd8\u6709\u4e13\u95e8\u7684\u6570\u636e\u5e93PhaSepDB\u3001DrLLPS\uff0c\u76f8\u5f53\u65b9\u4fbf\u4e86\uff01<\/strong>\u4eca\u5929\u5c0f\u4e91\u5c31\u5206\u4eab\u4e00\u4e2a6\u5206+\u7684\u5e72\u6e7f\u7ed3\u5408\u601d\u8def\u7ed9\u5c0f\u4f19\u4f34\u4eec\u6253\u4e2a\u6837\uff0c\u6587\u7ae0\u505a\u4e86\u80bf\u7624\u9884\u540e+\u5206\u578b\u5206\u6790+\u9a8c\u8bc1\u5b9e\u9a8c\uff0c\u521b\u65b0\u6027\u5230\u4f4d\uff0c\u6570\u636e\u4e5f\u5f88\u4e30\u5bcc\uff0c\u503c\u5f97\u4e00\u5b66\uff01<strong>\u76ee\u524d\u201c\u76f8\u5206\u79bb\u201d\u505a\u751f\u4fe1\u7684\u8fd8\u5f88\u5c11\uff0c\u5c0f\u4e91\u5df2\u5907\u597d\u57fa\u56e0\u96c6\uff0c\u611f\u5174\u8da3\u7684\u670b\u53cb\u6293\u7d27\u884c\u52a8\uff0c\u6362\u4e2a\u764c\u79cd\u53bb\u590d\u73b0\u5427\uff01<\/strong><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"alignnone size-medium wp-image-36511 aligncenter\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/2.jpg?resize=298%2C300\" alt=\"\" width=\"298\" height=\"300\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/2.jpg?resize=298%2C300 298w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/2.jpg?resize=150%2C150 150w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/2.jpg?resize=100%2C100 100w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/2.jpg?w=568 568w\" sizes=\"(max-width: 298px) 100vw, 298px\" data-recalc-dims=\"1\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"173\" class=\"wp-image-38073\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-2.png?resize=640%2C173\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-2.png?w=691 691w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-2.png?resize=300%2C81 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-2.png?resize=600%2C162 600w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/><\/p>\n<ul>\n<li>\u9898\u76ee\uff1a\u4e00\u4e2a\u4e0e\u524d\u5217\u817a\u764c\u60a3\u8005\u751f\u5316\u590d\u53d1\u548c\u80bf\u7624\u514d\u75ab\u73af\u5883\u76f8\u5173\u6db2-\u6db2\u76f8\u5206\u79bb\u76f8\u5173\u6307\u6570<\/li>\n<li>\u6742\u5fd7\uff1a<em>Int J Mol Sci.<\/em><\/li>\n<li>\u5f71\u54cd\u56e0\u5b50\uff1aIF=6.208<\/li>\n<li>\u53d1\u8868\u65f6\u95f4\uff1a2023\u5e743\u6708<\/li>\n<\/ul>\n<p><strong>\u7814\u7a76\u80cc\u666f<\/strong><\/p>\n<p>\u751f\u5316\u590d\u53d1(BCR)\u662f\u4e00\u4e2a\u8868\u660e\u524d\u5217\u817a\u764c(PCa)\u60a3\u8005\u7684\u9884\u540e\u4e0d\u826f\u7684\u6307\u6807\uff0c\u7136\u800c\uff0c\u5f88\u5c11\u6709\u516c\u8ba4\u7684\u76ee\u6807\u53ef\u4ee5\u6709\u52a9\u4e8e\u9884\u6d4b\u524d\u5217\u817a\u764c\u60a3\u8005\u7684BCRFS\u3002\u5df2\u6709\u7814\u7a76\u62a5\u9053\u4e86\u6db2-\u6db2\u76f8\u5206\u79bb\uff08LLPS\uff09\u5728\u524d\u5217\u817a\u764c\u6cbb\u7597\u548c\u8fdb\u5c55\u4e2d\u7684\u91cd\u8981\u6027\uff0c\u4f46\u96c6\u4e2d\u63a2\u8ba8LLPS\u4e0ePCa\u60a3\u8005BCR\u4e4b\u95f4\u76f8\u5173\u6027\u7684\u7814\u7a76\u4ecd\u7136\u6709\u9650\u3002<\/p>\n<p><strong>\u6570\u636e\u6765\u6e90<\/strong><\/p>\n<table>\n<tbody>\n<tr>\n<td><strong>\u6570\u636e\u96c6\/\u961f\u5217<\/strong><\/td>\n<td><strong>\u6570\u636e\u5e93<\/strong><\/td>\n<td><strong>\u6570\u636e\u7c7b\u578b<\/strong><\/td>\n<td><strong>\u6837\u672c\u4fe1\u606f<\/strong><\/td>\n<\/tr>\n<tr>\n<td>TCGA-PCa<\/td>\n<td>TCGA<\/td>\n<td>RNA-seq\u6570\u636e+\u4e34\u5e8a\u4fe1\u606f\uff08\u5305\u542bBCR\u6570\u636e\uff09<\/td>\n<td>405\u4f8b\u524d\u5217\u817a\u764c\u75c5\u4f8b\u6837\u672c<\/td>\n<\/tr>\n<tr>\n<td>GSE70770<\/td>\n<td>GEO<\/td>\n<td>RNA-seq\u6570\u636e+\u4e34\u5e8a\u4fe1\u606f\uff08\u5305\u542bBCR\u6570\u636e\uff09<\/td>\n<td>203\u4f8b\u524d\u5217\u817a\u764c\u6837\u672c<\/td>\n<\/tr>\n<tr>\n<td>LLPS\u76f8\u5173\u57fa\u56e0<\/td>\n<td>PhaSepDB<\/td>\n<td><\/td>\n<td>961\u4e2a\u57fa\u56e0<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u7814\u7a76\u601d\u8def<\/strong><\/p>\n<p>\u9996\u5148\u7b5b\u9009\u4e86\u5dee\u5f02\u8868\u8fbe\u7684\u6db2-\u6db2\u76f8\u5206\u79bb\u76f8\u5173\u57fa\u56e0(DELRGs)\uff0c\u7136\u540e\u901a\u8fc7\u5171\u8bc6\u805a\u7c7b\u5206\u6790\u786e\u5b9a\u4e86PCa\u7684\u4e09\u4e2aLLPS\u76f8\u5173\u5206\u5b50\u7c07\uff0c\u5e76\u8fdb\u884c\u4e9a\u578b\u95f4\u7684\u9884\u540e\u5206\u6790\u548c\u514d\u75ab\u6d78\u6da6\u5206\u6790\u3002\u518d\u901a\u8fc7LASSO cox\u56de\u5f52\u5206\u6790\u5efa\u7acb\u4e00\u4e2a\u65b0\u7684LLPS\u76f8\u5173\u6307\u6570\u6765\u9884\u6d4b\u751f\u5316\u590d\u53d1(BCR)\u65e0\u7624\u751f\u5b58\u7387(BCRFS)\u3002\u968f\u540e\u63a2\u7d22\u4e86LLPS\u76f8\u5173\u6307\u6570\u4e0e\u5206\u5b50\u4e9a\u578b\u3001\u80bf\u7624\u514d\u75ab\u5fae\u73af\u5883\u548c\u836f\u7269\u654f\u611f\u6027\u7684\u6f5c\u5728\u76f8\u5173\u6027\u3002\u6700\u540e\uff0c\u8fdb\u884c\u4e86\u5173\u952e\u57fa\u56e0FUS\u7684\u5b9e\u9a8c\u9a8c\u8bc1\uff0c\u4ee5\u9a8c\u8bc1FUS\u662fPCa\u7684\u6f5c\u5728\u751f\u7269\u6807\u5fd7\u7269\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"485\" class=\"wp-image-38074\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-3.png?resize=640%2C485\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-3.png?w=745 745w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-3.png?resize=300%2C228 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-3.png?resize=600%2C455 600w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/><\/p>\n<p><strong>\u4e3b\u8981\u7ed3\u679c<\/strong><\/p>\n<p><strong>1. \u57fa\u4e8e\u6db2-\u6db2\u76f8\u5206\u79bb\u7684\u80bf\u7624\u5206\u578b\u5206\u6790<\/strong><\/p>\n<p>\u9996\u5148\u5728TCGA\u961f\u5217\u4e2d\u7b5b\u9009\u83b7\u5f97\u4e86102\u4e2a\u5dee\u5f02\u8868\u8fbe\u7684\u6db2-\u6db2\u76f8\u5206\u79bb\u76f8\u5173\u57fa\u56e0(DELRGs)\uff08\u56fe1A\uff09\uff0c\u968f\u540e\u8fdb\u884c\u4e86\u5355\u53d8\u91cfCox\u56de\u5f52\u5206\u6790\u4ee5\u7b5b\u9009\u4e0e\u65e0BCR\u751f\u5b58\u7387(BCRFS)\u76f8\u5173\u7684\u9884\u540e\u6027DELRGs\u3002\u57fa\u4e8e\u8fd9\u4e9b\u57fa\u56e0\u8fdb\u884c\u4e00\u81f4\u6027\u805a\u7c7b\u5206\u6790\uff0c\u83b7\u5f97\u4e863\u4e2a\u57fa\u4e8eLLPS\u7684\u5206\u5b50\u7c07\uff08\u56fe1B\uff09\u3002\u901a\u8fc7KM\u751f\u5b58\u5206\u6790\u6bd4\u8f833\u4e2a\u4e9a\u578b\u95f4\u7684\u9884\u540e\u5dee\u5f02\uff08\u56fe1D\uff09\uff0c\u5e76\u6bd4\u8f833\u4e2a\u4e9a\u578b\u95f4\u7684PD-1\u548cPD-L1\u8868\u8fbe\u5dee\u5f02\uff08\u56fe1E\uff09\u3002\u7136\u540e\u4f7f\u7528\u4f30\u8ba1\u7b97\u6cd5\u548cCIBERSORT\u7b97\u6cd5\uff0c\u5206\u6790\u4e0d\u540cLLPS\u76f8\u5173\u5206\u5b50\u7c07\u95f4\u514d\u75ab\u6d78\u6da6\u7ec6\u80de\uff08\u56fe2A\uff09\u548c\u80bf\u7624\u5fae\u73af\u5883\u8bc4\u5206\u7684\u5dee\u5f02\uff08\u56fe2B\uff09\u3002<strong>\uff08ps\uff1a\u5dee\u5f02\u57fa\u56e0\u5206\u6790\u3001cox\u56de\u5f52\u5206\u6790\u3001\u805a\u7c7b\u5206\u6790\u3001KM\u66f2\u7ebf\u7ed8\u5236\u4e5f\u53ef\u4ee5\u7528\u5c0f\u4e91\u65b0\u5f00\u53d1\u7684\u96f6\u4ee3\u7801\u751f\u4fe1\u5206\u6790\u5c0f\u5de5\u5177\u5b9e\u73b0\uff0c\u4e91\u751f\u4fe1\u5206\u6790\u5de5\u5177\u5e73\u53f0\u5305\u542b\u8d85\u591a\u96f6\u4ee3\u7801\u5206\u6790\u548c\u7ed8\u56fe\u5c0f\u5de5\u5177\uff0c\u4e0a\u4f20\u6570\u636e\u4e00\u952e\u51fa\u56fe\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u6765\u5c1d\u8bd5\u54df\uff0c\u7f51\u5740\uff1ahttp:\/\/www.biocloudservice.com\/home.html\uff09<\/strong>\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"420\" height=\"416\" class=\"wp-image-38075\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-4.png?resize=420%2C416\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-4.png?w=420 420w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-4.png?resize=300%2C297 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-4.png?resize=150%2C150 150w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-4.png?resize=100%2C100 100w\" sizes=\"(max-width: 420px) 100vw, 420px\" data-recalc-dims=\"1\" \/> <img decoding=\"async\" loading=\"lazy\" width=\"357\" height=\"371\" class=\"wp-image-38076\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-5.png?resize=357%2C371\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-5.png?w=357 357w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-5.png?resize=289%2C300 289w\" sizes=\"(max-width: 357px) 100vw, 357px\" data-recalc-dims=\"1\" \/> <img decoding=\"async\" loading=\"lazy\" width=\"408\" height=\"355\" class=\"wp-image-38077\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-6.png?resize=408%2C355\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-6.png?w=408 408w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-6.png?resize=300%2C261 300w\" sizes=\"(max-width: 408px) 100vw, 408px\" data-recalc-dims=\"1\" \/> <img decoding=\"async\" loading=\"lazy\" width=\"392\" height=\"344\" class=\"wp-image-38078\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-7.png?resize=392%2C344\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-7.png?w=392 392w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-7.png?resize=300%2C263 300w\" sizes=\"(max-width: 392px) 100vw, 392px\" data-recalc-dims=\"1\" \/><\/p>\n<p>\u56fe1 \u57fa\u4e8e\u6db2-\u6db2\u76f8\u5206\u79bb\u7684\u80bf\u7624\u5206\u578b\u5206\u6790<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"484\" class=\"wp-image-38079\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-8.png?resize=640%2C484\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-8.png?w=728 728w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-8.png?resize=300%2C227 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-8.png?resize=600%2C453 600w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/> <img decoding=\"async\" loading=\"lazy\" width=\"365\" height=\"342\" class=\"wp-image-38080\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-9.png?resize=365%2C342\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-9.png?w=365 365w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-9.png?resize=300%2C281 300w\" sizes=\"(max-width: 365px) 100vw, 365px\" data-recalc-dims=\"1\" \/><\/p>\n<p>\u56fe2\u4e9a\u578b\u95f4\u80bf\u7624\u5fae\u73af\u5883\u5206\u6790<\/p>\n<p><strong>2. \u4e00\u79cd\u65b0\u7684LLPS\u6307\u6570\u7684\u5f00\u53d1\u548c\u9a8c\u8bc1<\/strong><\/p>\n<p>\u9996\u5148\u5728TCGA\u961f\u5217\u4e2d\u8fdb\u884c\u5355\u53d8\u91cfCox\u5206\u6790\uff0c\u7b5b\u9009\u5f97\u523013\u4e2aBCR\u9884\u540e\u76f8\u5173DELRGs\uff08\u56fe3C\uff09\u3002\u7136\u540e\u518d\u5229\u7528LASSO\u56de\u5f52\u5206\u6790\u6700\u7ec8\u7b5b\u9009\u51fa6\u4e2aDELRGs\uff0c\u5e76\u6784\u5efa\u4e86\u4e00\u4e2a\u65b0\u7684\u4e0eLLPS\u76f8\u5173\u7684\u9884\u540e\u6307\u6570\u6765\u9884\u6d4b\u524d\u5217\u817a\u764c\u60a3\u8005\u7684BCRFS\uff08\u56fe3D, E\uff09\u3002\u6839\u636e\u98ce\u9669\u8bc4\u5206\u4e2d\u4f4d\u6570\uff0c\u5c06\u6240\u6709\u75c5\u4f8b\u5206\u4e3a\u4e24\u4e2a\u9ad8\u98ce\u9669\u548c\u4f4e\u98ce\u9669\u8bc4\u5206\u4e9a\u7ec4\uff0c\u8fdb\u884cKM\u751f\u5b58\u5206\u6790\u5e76\u901a\u8fc7ROC\u66f2\u7ebf\u6765\u8bc4\u4f30\u5176\u9884\u6d4b\u6027\u80fd\uff08\u56fe3A, B\uff09\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"519\" height=\"426\" class=\"wp-image-38081\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-10.png?resize=519%2C426\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-10.png?w=519 519w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-10.png?resize=300%2C246 300w\" sizes=\"(max-width: 519px) 100vw, 519px\" data-recalc-dims=\"1\" \/> <img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"346\" class=\"wp-image-38082\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-11.png?resize=640%2C346\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-11.png?w=808 808w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-11.png?resize=300%2C162 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-11.png?resize=768%2C415 768w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-11.png?resize=600%2C325 600w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/> <img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"296\" class=\"wp-image-38083\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-12.png?resize=640%2C296\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-12.png?w=911 911w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-12.png?resize=300%2C139 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-12.png?resize=768%2C355 768w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-12.png?resize=600%2C277 600w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/><\/p>\n<p>\u56fe3 LLPS\u6307\u6570\u7684\u5f00\u53d1\u548c\u9a8c\u8bc1<\/p>\n<p><strong>3. \u98ce\u9669\u8bc4\u5206\u4e0e\u4e34\u5e8a\u75c5\u7406\u7279\u5f81\u3001\u80bf\u7624\u5e72\u7ec6\u80de\u4e0e\u80bf\u7624\u514d\u75ab\u5fae\u73af\u5883\u7684\u76f8\u5173\u6027\u5206\u6790<\/strong><\/p>\n<p>\u901a\u8fc7\u5355\u53d8\u91cf\u548c\u591a\u53d8\u91cf\u56de\u5f52\u8fdb\u884c\u72ec\u7acb\u9884\u540e\u5206\u6790\uff0c\u7ed3\u679c\u663e\u793a\u98ce\u9669\u8bc4\u5206\u662fPCa\u60a3\u8005\u7684\u72ec\u7acb\u9884\u6d4b\u56e0\u5b50\uff08\u56fe4A, B\uff09\u3002\u968f\u540e\u5206\u6790\u4e86\u98ce\u9669\u8bc4\u5206\u4e0e\u4e00\u4e9b\u4e34\u5e8a\u75c5\u7406\u7279\u5f81\uff08\u56fe4C\uff09\u548c\u5e72\u7ec6\u80de\u8bc4\u5206\u7684\u76f8\u5173\u6027\uff08\u56fe4D, E\uff09\u3002\u5229\u7528ssGSEA\u5206\u6790\u6765\u91cf\u5316\u514d\u75ab\u529f\u80fd\uff0c\u8bc4\u4f30\u4e0d\u540c\u98ce\u9669\u4e9a\u7ec4\u95f4\u7684\u514d\u75ab\u6d78\u6da6\u60c5\u51b5\uff08\u56fe4F\uff09\u3002<strong>\uff08ps\uff1a\u591a\u79cd\u514d\u75ab\u6d78\u6da6\u5206\u6790\u4e5f\u53ef\u4ee5\u7528\u4e91\u751f\u4fe1\u5e73\u53f0\u5206\u6790\u5de5\u5177\u5b9e\u73b0\u54e6\uff0c\u7f51\u5740\uff1a<a href=\"http:\/\/www.biocloudservice.com\/home.html\">http:\/\/www.biocloudservice.com\/home.html<\/a>\uff0c\u6b22\u8fce\u670b\u53cb\u4eec\u6765\u63a2\u7d22~ ~\uff09<\/strong><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"227\" class=\"wp-image-38084\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-13.png?resize=640%2C227\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-13.png?w=877 877w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-13.png?resize=300%2C106 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-13.png?resize=768%2C272 768w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-13.png?resize=600%2C213 600w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/> <img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"205\" class=\"wp-image-38085\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-14.png?resize=640%2C205\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-14.png?w=932 932w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-14.png?resize=300%2C96 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-14.png?resize=768%2C246 768w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-14.png?resize=600%2C192 600w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/> <img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"301\" class=\"wp-image-38086\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-15.png?resize=640%2C301\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-15.png?w=903 903w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-15.png?resize=300%2C141 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-15.png?resize=768%2C361 768w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-15.png?resize=600%2C282 600w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/> <img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"442\" class=\"wp-image-38087\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-16.png?resize=640%2C442\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-16.png?w=718 718w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-16.png?resize=300%2C207 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-16.png?resize=600%2C414 600w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/><\/p>\n<p>\u56fe4 \u98ce\u9669\u8bc4\u5206\u4e0e\u4e34\u5e8a\u75c5\u7406\u7279\u5f81\u3001\u80bf\u7624\u5e72\u7ec6\u80de\u4e0e\u80bf\u7624\u514d\u75ab\u5fae\u73af\u5883\u7684\u76f8\u5173\u6027\u5206\u6790<\/p>\n<p><strong>4. \u5173\u952e\u57fa\u56e0\u8868\u8fbe\u548c\u529f\u80fd\u9a8c\u8bc1\u5b9e\u9a8c<\/strong><\/p>\n<p>\u4f5c\u8005\u901a\u8fc7qRT-PCR\u68c0\u6d4b\u524d\u5217\u817a\u764c\u7ec6\u80de\u548c\u6b63\u5e38\u524d\u5217\u817a\u4e0a\u76ae\u7ec6\u80de\u4e2d6\u4e2a\u6a21\u578b\u57fa\u56e0\u7684mRNA\u8868\u8fbe\u6c34\u5e73\uff08\u56fe5A\uff09\uff0c\u901a\u8fc7IHC\u67d3\u8272\u6765\u5206\u6790\u8fd9\u4e9b6\u4e2a\u57fa\u56e0\u5728\u524d\u5217\u817a\u589e\u751f\u548c\u524d\u5217\u817a\u764c\u7ec4\u7ec7\u4e2d\u86cb\u767d\u8868\u8fbe\u7684\u5dee\u5f02\uff08\u56fe5B\uff09\u3002\u6839\u636e\u7ed3\u679c\u9009\u62e9\u5173\u952e\u57fa\u56e0FUS\u4f5c\u4e3a\u540e\u7eed\u5b9e\u9a8c\u76ee\u6807\uff0c\u6c89\u9ed8\u5176\u8868\u8fbe\u540e\uff0c\u901a\u8fc7CCK8\u5b9e\u9a8c\u68c0\u6d4b\u7ec6\u80de\u589e\u6b96\uff08\u56fe5C\uff09\uff0cTranswell\u5b9e\u9a8c\u68c0\u6d4b\u7ec6\u80de\u8fc1\u79fb\u548c\u4fb5\u88ad\uff08\u56fe5E-H\uff09\uff0c\u6d41\u5f0f\u68c0\u6d4b\u7ec6\u80de\u51cb\u4ea1\uff08\u56fe5I, 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src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-19.png?resize=212%2C227\" data-recalc-dims=\"1\" \/> <img decoding=\"async\" loading=\"lazy\" width=\"640\" height=\"349\" class=\"wp-image-38091\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-20.png?resize=640%2C349\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-20.png?w=667 667w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-20.png?resize=300%2C164 300w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-20.png?resize=600%2C327 600w\" sizes=\"(max-width: 640px) 100vw, 640px\" data-recalc-dims=\"1\" \/> <img decoding=\"async\" loading=\"lazy\" width=\"213\" height=\"278\" class=\"wp-image-38092\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-21.png?resize=213%2C278\" data-recalc-dims=\"1\" \/> <img decoding=\"async\" loading=\"lazy\" width=\"523\" height=\"311\" class=\"wp-image-38093\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-22.png?resize=523%2C311\" srcset=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-22.png?w=523 523w, https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-22.png?resize=300%2C178 300w\" sizes=\"(max-width: 523px) 100vw, 523px\" data-recalc-dims=\"1\" \/> <img decoding=\"async\" loading=\"lazy\" width=\"215\" height=\"257\" class=\"wp-image-38094\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/04\/word-image-38069-23.png?resize=215%2C257\" data-recalc-dims=\"1\" 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10\u5206+\uff01\u57fa\u5e95\u819c\u65b9\u5411\uff0c\u80bf\u7624\u9884\u540e\u6a21\u578b\u6784\u5efa+\u5355\u7ec6\u80de\u5206\u6790+\u9a8c\u8bc1\u5b9e\u9a8c\u601d\u8def\uff0c\u5e72\u6e7f\u7ed3\u5408\u4e0d\u8e6d\u5927\u70ed\u70b9\u4e5f\u80fd\u62ff\u9ad8\u5206\uff0c\u5feb\u6765\u6284\u4f5c\u4e1a\uff01<\/p>\n<p>2. \u75c5\u7406\u5206\u6790\u4e0e\u751f\u4fe1\u5f3a\u5f3a\u8054\u624b\uff0c\u5355\u57fa\u56e0\u5206\u6790+\u514d\u75ab\u5206\u6790\u76f4\u8fbe5.7\u5206+\uff0c\u521b\u65b0\u6027\u7edd\u7edd\u5b50\uff0c\u4e0d\u6015\u601d\u8def\u649e\u8f66!\uff01<\/p>\n<p>3. \u5206\u5b50\u673a\u5236\u505a\u751f\u4fe1\u53c8\u51fa\u65b0\u65b9\u5411\u2014\u2014\u7c7b\u6cdb\u7d20\u5316\u4fee\u9970\uff0c\u7ec6\u80de\u5b9e\u9a8c\u5f15\u5165+\u80bf\u7624\u5206\u578b\u5206\u6790+\u673a\u5668\u5b66\u4e60\u9884\u540e\u6a21\u578b\u6784\u5efa=5\u5206+\uff0c\u601d\u8def\u65b0\u9896\u597d\u590d\u73b0\uff0c\u7b49\u4f60\u6765\u64a9\uff01<\/p>\n<p>4. 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