{"id":43158,"date":"2024-06-01T23:39:25","date_gmt":"2024-06-01T15:39:25","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=43158"},"modified":"2024-06-01T23:39:27","modified_gmt":"2024-06-01T15:39:27","slug":"%e5%8d%95%e7%bb%86%e8%83%9e%e5%88%86%e6%9e%90%e5%b7%a5%e5%85%b7%e5%af%b9%e6%af%94%e8%a1%a8","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=43158","title":{"rendered":"\u5355\u7ec6\u80de\u5206\u6790\u5de5\u5177\u5bf9\u6bd4\u8868"},"content":{"rendered":"\n

\u5355\u7ec6\u80de\u5206\u6790\u5df2\u7ecf\u6210\u4e3a\u73b0\u4ee3\u751f\u7269\u533b\u5b66\u7814\u7a76\u7684\u91cd\u8981\u5de5\u5177\uff0c\u80fd\u591f\u4ee5\u5355\u7ec6\u80de\u5206\u8fa8\u7387\u63ed\u793a\u7ec4\u7ec7\u548c\u5668\u5b98\u7684\u590d\u6742\u6027\u3002\u968f\u7740\u6280\u672f\u7684\u53d1\u5c55\uff0c\u8bb8\u591a\u5f3a\u5927\u7684\u5de5\u5177\u548c\u8f6f\u4ef6\u5305\u88ab\u5f00\u53d1\u51fa\u6765\uff0c\u7528\u4e8e\u5904\u7406\u548c\u5206\u6790\u5355\u7ec6\u80de\u6570\u636e\u3002\u4ee5\u4e0b\u662f\u4e00\u4e9b\u5e38\u7528\u7684\u5355\u7ec6\u80de\u5206\u6790\u5de5\u5177\u53ca\u5176\u7b80\u4ecb\uff1a<\/p>\n\n\n\n

<\/p>\n\n\n\n

<\/h3>\n\n\n\n

\u5355\u7ec6\u80de\u5206\u6790\u5de5\u5177\u7684\u9009\u62e9\u72b9\u5982\u5728\u4e00\u5e45\u590d\u6742\u800c\u53c8\u7f8e\u4e3d\u7684\u5b66\u672f\u753b\u5377\u4e2d\u6311\u9009\u6700\u9002\u5408\u7684\u8272\u5f69\uff0c\u6bcf\u4e00\u4e2a\u5de5\u5177\u90fd\u5728\u5176\u4e2d\u626e\u6f14\u7740\u4e0d\u53ef\u6216\u7f3a\u7684\u89d2\u8272\u3002\u8ba9\u6211\u4eec\u6df1\u5165\u63a2\u8ba8\u5e76\u6bd4\u8f83\u8fd9\u4e9b\u5de5\u5177\uff0c\u770b\u770b\u5b83\u4eec\u5982\u4f55\u5728\u8fd9\u7247\u7814\u7a76\u7684\u7eff\u6d32\u4e2d\u4e92\u76f8\u4ea4\u7ec7\uff0c\u5f62\u6210\u4e00\u4e2a\u590d\u6742\u4e14\u5f15\u4eba\u5165\u80dc\u7684\u77e5\u8bc6\u8ff7\u5bab\u3002<\/p>\n\n\n\n

\u5de5\u5177<\/th>\u529f\u80fd<\/th>\u7f16\u7a0b\u8bed\u8a00<\/th>\u4e3b\u8981\u7279\u70b9<\/th>\u4f18\u70b9<\/th>\u7f3a\u70b9<\/th><\/tr><\/thead>
Seurat<\/strong><\/td>\u6570\u636e\u9884\u5904\u7406\u3001\u9ad8\u7ef4\u964d\u7ef4\u3001\u805a\u7c7b\u5206\u6790\u3001\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u3001\u6570\u636e\u6574\u5408<\/td>R<\/td>\u591a\u529f\u80fd\uff0c\u9002\u5408\u5927\u591a\u6570\u5355\u7ec6\u80de RNA \u6d4b\u5e8f\u6570\u636e<\/td>\u529f\u80fd\u5168\u9762\uff0c\u793e\u533a\u6d3b\u8dc3\uff0c\u6587\u6863\u548c\u6559\u7a0b\u4e30\u5bcc<\/td>\u5bf9\u5927\u6570\u636e\u96c6\u5904\u7406\u6027\u80fd\u7a0d\u900a\u8272<\/td><\/tr>
Scanpy<\/strong><\/td>\u6570\u636e\u9884\u5904\u7406\u3001\u9ad8\u7ef4\u964d\u7ef4\u3001\u805a\u7c7b\u5206\u6790\u3001\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u3001\u53ef\u89c6\u5316<\/td>Python<\/td>\u5904\u7406\u5927\u89c4\u6a21\u6570\u636e\u96c6\u6027\u80fd\u4f18\u8d8a\uff0c\u96c6\u6210\u4e86\u6700\u65b0\u7b97\u6cd5<\/td>\u9ad8\u6548\u5904\u7406\u5927\u89c4\u6a21\u6570\u636e\uff0c\u529f\u80fd\u5168\u9762<\/td>\u5b66\u4e60\u66f2\u7ebf\u9661\u5ced\uff0c\u9700\u8981 Python \u7f16\u7a0b\u57fa\u7840<\/td><\/tr>
Monocle<\/strong><\/td>\u8f68\u8ff9\u63a8\u65ad\u3001\u7ec6\u80de\u547d\u8fd0\u5206\u5316\u8def\u5f84\u8bc6\u522b\u3001\u5dee\u5f02\u8868\u8fbe\u5206\u6790<\/td>R<\/td>\u4e13\u6ce8\u4e8e\u65f6\u95f4\u5e8f\u5217\u548c\u8f68\u8ff9\u5206\u6790\uff0c\u9002\u7528\u4e8e\u53d1\u80b2\u751f\u7269\u5b66\u7814\u7a76<\/td>\u64c5\u957f\u8f68\u8ff9\u5206\u6790\uff0c\u9002\u5408\u53d1\u80b2\u7814\u7a76<\/td>\u529f\u80fd\u76f8\u5bf9\u5355\u4e00\uff0c\u4e0d\u9002\u5408\u6240\u6709\u7c7b\u578b\u7684\u5355\u7ec6\u80de\u5206\u6790<\/td><\/tr>
Cell Ranger<\/strong><\/td>\u6570\u636e\u9884\u5904\u7406\u3001\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u751f\u6210\u3001\u805a\u7c7b\u5206\u6790\u3001\u53ef\u89c6\u5316<\/td>\u8f6f\u4ef6<\/td>\u4e0e 10x Genomics \u5e73\u53f0\u9ad8\u5ea6\u517c\u5bb9\uff0c\u5904\u7406\u6570\u636e\u9ad8\u6548<\/td>\u9ad8\u5ea6\u517c\u5bb9 10x Genomics \u5e73\u53f0\uff0c\u5904\u7406\u5feb\u901f<\/td>\u4e3b\u8981\u9002\u7528\u4e8e 10x Genomics \u6570\u636e<\/td><\/tr>
SingleR<\/strong><\/td>\u81ea\u52a8\u6ce8\u91ca\u5355\u7ec6\u80de RNA \u6d4b\u5e8f\u6570\u636e<\/td>R<\/td>\u6839\u636e\u53c2\u8003\u6570\u636e\u96c6\u5bf9\u5355\u7ec6\u80de\u8fdb\u884c\u6ce8\u91ca\uff0c\u652f\u6301\u591a\u79cd\u53c2\u8003\u6570\u636e\u96c6<\/td>\u6ce8\u91ca\u51c6\u786e\uff0c\u4f7f\u7528\u7b80\u5355<\/td>\u529f\u80fd\u76f8\u5bf9\u5355\u4e00\uff0c\u53ea\u9002\u7528\u4e8e\u7ec6\u80de\u6ce8\u91ca<\/td><\/tr>
SCENIC<\/strong><\/td>\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u63a8\u65ad\u3001\u7ec6\u80de\u7c7b\u578b\u8bc6\u522b\u3001\u5dee\u5f02\u8868\u8fbe\u5206\u6790<\/td>R<\/td>\u63d0\u4f9b\u57fa\u4e8e\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u7684\u6df1\u5165\u751f\u7269\u5b66\u6d1e\u5bdf<\/td>\u6df1\u5165\u7684\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u5206\u6790\uff0c\u529f\u80fd\u5f3a\u5927<\/td>\u5b66\u4e60\u66f2\u7ebf\u9661\u5ced\uff0c\u9700\u8981\u751f\u7269\u4fe1\u606f\u5b66\u77e5\u8bc6<\/td><\/tr>
MAESTRO<\/strong><\/td>\u591a\u7ec4\u5b66\u6570\u636e\u6574\u5408\u3001\u805a\u7c7b\u5206\u6790\u3001\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u3001\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u63a8\u65ad<\/td>Python<\/td>\u652f\u6301\u591a\u7ec4\u5b66\u6570\u636e\uff0c\u529f\u80fd\u5168\u9762<\/td>\u591a\u7ec4\u5b66\u5206\u6790\uff0c\u529f\u80fd\u4e30\u5bcc<\/td>\u5b89\u88c5\u548c\u4f7f\u7528\u590d\u6742\uff0c\u9700\u6c42\u8f83\u9ad8\u7684\u8ba1\u7b97\u8d44\u6e90<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\u603b\u7ed3<\/h3>\n\n\n\n

<\/p>\n\n\n\n

<\/p>\n\n\n\n

<\/p>\n\n\n\n

<\/p>\n\n\n\n

1. Seurat<\/h3>\n\n\n\n

\u7b80\u4ecb<\/strong>: Seurat \u662f\u4e00\u4e2a\u529f\u80fd\u5f3a\u5927\u7684 R \u5305\uff0c\u5e7f\u6cdb\u7528\u4e8e\u5355\u7ec6\u80de RNA \u6d4b\u5e8f\uff08scRNA-seq\uff09\u6570\u636e\u7684\u5206\u6790\u548c\u53ef\u89c6\u5316\u3002<\/p>\n\n\n\n

\u4e3b\u8981\u529f\u80fd<\/strong>:<\/p>\n\n\n\n

    \n
  • \u6570\u636e\u9884\u5904\u7406\uff08\u5f52\u4e00\u5316\u3001\u5f52\u4e00\u5316\u3001\u53bb\u9664\u6279\u6b21\u6548\u5e94\uff09<\/li>\n\n\n\n
  • \u9ad8\u7ef4\u6570\u636e\u964d\u7ef4\uff08PCA\u3001t-SNE\u3001UMAP\uff09<\/li>\n\n\n\n
  • \u805a\u7c7b\u5206\u6790<\/li>\n\n\n\n
  • \u5dee\u5f02\u8868\u8fbe\u5206\u6790<\/li>\n\n\n\n
  • \u6570\u636e\u6574\u5408\uff08\u591a\u6837\u672c\u6216\u591a\u7ec4\u5b66\u6570\u636e\uff09<\/li>\n<\/ul>\n\n\n\n

    \u4f18\u70b9<\/strong>: \u529f\u80fd\u5168\u9762\uff0c\u793e\u533a\u6d3b\u8dc3\uff0c\u6587\u6863\u548c\u6559\u7a0b\u4e30\u5bcc\u3002<\/p>\n\n\n\n

    \u5b89\u88c5<\/strong>:<\/p>\n\n\n\n

    install.packages(\"Seurat\")<\/code><\/pre>\n\n\n\n
    2. Scanpy<\/h3>\n\n\n\n
    \u7b80\u4ecb<\/strong>: Scanpy \u662f\u4e00\u4e2a\u57fa\u4e8e Python \u7684\u5355\u7ec6\u80de RNA \u6d4b\u5e8f\u6570\u636e\u5206\u6790\u5de5\u5177\uff0c\u7279\u522b\u9002\u5408\u5904\u7406\u5927\u89c4\u6a21\u6570\u636e\u96c6\u3002<\/p>\n\n\n\n
    \u4e3b\u8981\u529f\u80fd<\/strong>:<\/p>\n\n\n\n
      \n
    • \u6570\u636e\u9884\u5904\u7406<\/li>\n\n\n\n
    • \u9ad8\u7ef4\u6570\u636e\u964d\u7ef4<\/li>\n\n\n\n
    • \u805a\u7c7b\u5206\u6790<\/li>\n\n\n\n
    • \u5dee\u5f02\u8868\u8fbe\u5206\u6790<\/li>\n\n\n\n
    • \u53ef\u89c6\u5316<\/li>\n<\/ul>\n\n\n\n
      \u4f18\u70b9<\/strong>: \u5904\u7406\u5927\u89c4\u6a21\u6570\u636e\u96c6\u6027\u80fd\u4f18\u8d8a\uff0c\u96c6\u6210\u4e86\u8bb8\u591a\u6700\u65b0\u7684\u7b97\u6cd5\u3002<\/p>\n\n\n\n
      \u5b89\u88c5<\/strong>:<\/p>\n\n\n\n
      pip install scanpy<\/code><\/pre>\n\n\n\n
      3. Monocle<\/h3>\n\n\n\n
      \u7b80\u4ecb<\/strong>: Monocle \u662f\u4e00\u4e2a R \u5305\uff0c\u4e13\u6ce8\u4e8e\u5355\u7ec6\u80de\u8f68\u8ff9\u63a8\u65ad\u548c\u65f6\u95f4\u5e8f\u5217\u5206\u6790\u3002<\/p>\n\n\n\n
      \u4e3b\u8981\u529f\u80fd<\/strong>:<\/p>\n\n\n\n
        \n
      • \u8f68\u8ff9\u63a8\u65ad<\/li>\n\n\n\n
      • \u7ec6\u80de\u547d\u8fd0\u5206\u5316\u8def\u5f84\u8bc6\u522b<\/li>\n\n\n\n
      • \u5dee\u5f02\u8868\u8fbe\u5206\u6790<\/li>\n<\/ul>\n\n\n\n
        \u4f18\u70b9<\/strong>: \u64c5\u957f\u65f6\u95f4\u5e8f\u5217\u548c\u8f68\u8ff9\u5206\u6790\uff0c\u9002\u7528\u4e8e\u53d1\u80b2\u751f\u7269\u5b66\u7814\u7a76\u3002<\/p>\n\n\n\n
        \u5b89\u88c5<\/strong>:<\/p>\n\n\n\n
        install.packages(\"monocle3\")<\/code><\/pre>\n\n\n\n
        4. Cell Ranger<\/h3>\n\n\n\n
        \u7b80\u4ecb<\/strong>: Cell Ranger \u662f\u7531 10x Genomics \u63d0\u4f9b\u7684\u5206\u6790\u8f6f\u4ef6\uff0c\u7528\u4e8e\u5904\u7406 10x Genomics \u5355\u7ec6\u80de RNA \u6d4b\u5e8f\u6570\u636e\u3002<\/p>\n\n\n\n
        \u4e3b\u8981\u529f\u80fd<\/strong>:<\/p>\n\n\n\n
          \n
        • \u6570\u636e\u9884\u5904\u7406\uff08\u8bfb\u6570\u5bf9\u9f50\u3001\u53bb\u91cd\u590d\uff09<\/li>\n\n\n\n
        • \u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u751f\u6210<\/li>\n\n\n\n
        • \u805a\u7c7b\u5206\u6790\u548c\u53ef\u89c6\u5316<\/li>\n<\/ul>\n\n\n\n
          \u4f18\u70b9<\/strong>: \u4e0e 10x Genomics \u5e73\u53f0\u9ad8\u5ea6\u517c\u5bb9\uff0c\u5904\u7406\u6570\u636e\u9ad8\u6548\u3002<\/p>\n\n\n\n
          \u5b89\u88c5<\/strong>: \u4ece 10x Genomics \u7f51\u7ad9\u4e0b\u8f7d\u5e76\u6309\u7167\u8bf4\u660e\u5b89\u88c5\u3002<\/p>\n\n\n\n
          5. SingleR<\/h3>\n\n\n\n
          \u7b80\u4ecb<\/strong>: SingleR \u662f\u4e00\u4e2a R \u5305\uff0c\u7528\u4e8e\u5355\u7ec6\u80de RNA \u6d4b\u5e8f\u6570\u636e\u7684\u81ea\u52a8\u6ce8\u91ca\u3002<\/p>\n\n\n\n
          \u4e3b\u8981\u529f\u80fd<\/strong>:<\/p>\n\n\n\n
            \n
          • \u6839\u636e\u53c2\u8003\u6570\u636e\u96c6\u5bf9\u5355\u7ec6\u80de\u8fdb\u884c\u6ce8\u91ca<\/li>\n\n\n\n
          • \u652f\u6301\u591a\u79cd\u53c2\u8003\u6570\u636e\u96c6\uff08\u5982\u4eba\u7c7b\u548c\u5c0f\u9f20\uff09<\/li>\n<\/ul>\n\n\n\n
            \u4f18\u70b9<\/strong>: \u6ce8\u91ca\u51c6\u786e\uff0c\u4f7f\u7528\u7b80\u5355\u3002<\/p>\n\n\n\n
            \u5b89\u88c5<\/strong>:<\/p>\n\n\n\n
            install.packages(\"SingleR\")<\/code><\/pre>\n\n\n\n
            6. SCENIC<\/h3>\n\n\n\n
            \u7b80\u4ecb<\/strong>: SCENIC\uff08Single-Cell Regulatory Network Inference and Clustering\uff09\u662f\u4e00\u4e2a\u7528\u4e8e\u5355\u7ec6\u80de\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u63a8\u65ad\u548c\u7ec6\u80de\u7c7b\u578b\u8bc6\u522b\u7684\u5de5\u5177\u3002<\/p>\n\n\n\n
            \u4e3b\u8981\u529f\u80fd<\/strong>:<\/p>\n\n\n\n
              \n
            • \u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u63a8\u65ad<\/li>\n\n\n\n
            • \u7ec6\u80de\u7c7b\u578b\u8bc6\u522b<\/li>\n\n\n\n
            • \u5dee\u5f02\u8868\u8fbe\u5206\u6790<\/li>\n<\/ul>\n\n\n\n
              \u4f18\u70b9<\/strong>: \u63d0\u4f9b\u57fa\u4e8e\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u7684\u6df1\u5165\u751f\u7269\u5b66\u6d1e\u5bdf\u3002<\/p>\n\n\n\n
              \u5b89\u88c5<\/strong>:<\/p>\n\n\n\n
              if (!requireNamespace(\"BiocManager\", quietly = TRUE))\n    install.packages(\"BiocManager\")\nBiocManager::install(\"SCENIC\")<\/code><\/pre>\n\n\n\n
              7. MAESTRO<\/h3>\n\n\n\n
              \u7b80\u4ecb<\/strong>: MAESTRO \u662f\u4e00\u4e2a\u591a\u7ec4\u5b66\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u5de5\u5177\uff0c\u652f\u6301 scRNA-seq\u3001scATAC-seq \u7b49\u591a\u79cd\u6570\u636e\u7c7b\u578b\u3002<\/p>\n\n\n\n
              \u4e3b\u8981\u529f\u80fd<\/strong>:<\/p>\n\n\n\n
                \n
              • \u591a\u7ec4\u5b66\u6570\u636e\u6574\u5408<\/li>\n\n\n\n
              • \u805a\u7c7b\u5206\u6790<\/li>\n\n\n\n
              • \u5dee\u5f02\u8868\u8fbe\u5206\u6790<\/li>\n\n\n\n
              • \u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u63a8\u65ad<\/li>\n<\/ul>\n\n\n\n
                \u4f18\u70b9<\/strong>: \u652f\u6301\u591a\u7ec4\u5b66\u6570\u636e\uff0c\u529f\u80fd\u5168\u9762\u3002<\/p>\n\n\n\n
                \u5b89\u88c5<\/strong>:<\/p>\n\n\n\n
                # MAESTRO \u662f\u4e00\u4e2a Python \u5305\npip install MAESTRO<\/code><\/pre>\n\n\n\n
                \u603b\u7ed3<\/h3>\n\n\n\n
                \u4e0a\u8ff0\u5de5\u5177\u5404\u6709\u4f18\u7f3a\u70b9\uff0c\u9009\u62e9\u9002\u5408\u81ea\u5df1\u7814\u7a76\u9700\u6c42\u7684\u5de5\u5177\u81f3\u5173\u91cd\u8981\u3002Seurat \u548c Scanpy \u662f\u4e24\u4e2a\u529f\u80fd\u5168\u9762\u4e14\u5e7f\u6cdb\u4f7f\u7528\u7684\u5de5\u5177\uff0c\u9002\u5408\u5927\u591a\u6570\u5355\u7ec6\u80de RNA \u6d4b\u5e8f\u6570\u636e\u5206\u6790\u3002Monocle \u548c SCENIC \u5219\u9002\u5408\u7279\u5b9a\u5206\u6790\uff0c\u5982\u8f68\u8ff9\u63a8\u65ad\u548c\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u5206\u6790\u3002Cell Ranger \u662f\u5904\u7406 10x Genomics \u6570\u636e\u7684\u5fc5\u5907\u5de5\u5177\uff0c\u800c SingleR \u5219\u5728\u81ea\u52a8\u6ce8\u91ca\u65b9\u9762\u8868\u73b0\u51fa\u8272\u3002MAESTRO \u4f5c\u4e3a\u4e00\u4e2a\u591a\u7ec4\u5b66\u5de5\u5177\uff0c\u9002\u5408\u9700\u8981\u6574\u5408\u591a\u79cd\u5355\u7ec6\u80de\u6570\u636e\u7684\u7814\u7a76\u8005\u3002<\/p>\n\n\n\n
                \u901a\u8fc7\u5408\u7406\u9009\u62e9\u548c\u7ec4\u5408\u8fd9\u4e9b\u5de5\u5177\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u6df1\u5165\u89e3\u6790\u5355\u7ec6\u80de\u6570\u636e\uff0c\u63ed\u793a\u7ec6\u80de\u5f02\u8d28\u6027\u548c\u590d\u6742\u751f\u7269\u5b66\u8fc7\u7a0b\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"
                \u5355\u7ec6\u80de\u5206\u6790\u5df2\u7ecf\u6210\u4e3a\u73b0\u4ee3\u751f\u7269\u533b\u5b66\u7814\u7a76\u7684\u91cd\u8981\u5de5\u5177\uff0c\u80fd\u591f\u4ee5\u5355\u7ec6\u80de\u5206\u8fa8\u7387\u63ed\u793a\u7ec4\u7ec7\u548c\u5668\u5b98\u7684\u590d\u6742\u6027\u3002\u968f\u7740\u6280\u672f\u7684\u53d1\u5c55\uff0c\u8bb8\u591a\u5f3a […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/43158"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=43158"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/43158\/revisions"}],"predecessor-version":[{"id":43159,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/43158\/revisions\/43159"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=43158"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=43158"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=43158"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}