{"id":43163,"date":"2024-06-01T23:44:45","date_gmt":"2024-06-01T15:44:45","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=43163"},"modified":"2024-06-02T00:11:41","modified_gmt":"2024-06-01T16:11:41","slug":"43163","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=43163","title":{"rendered":"\u6570\u636e\u6e05\u6d17\uff1a\u751f\u7269\u4fe1\u606f\u5b66\u4e2d\u7684\u5173\u952e\u6b65\u9aa4"},"content":{"rendered":"\n<h3 class=\"wp-block-heading\"><\/h3>\n\n\n\n<p>\u6570\u636e\u6e05\u6d17\u662f\u751f\u7269\u4fe1\u606f\u5b66\u7814\u7a76\u4e2d\u4e0d\u53ef\u6216\u7f3a\u7684\u4e00\u6b65\u3002\u65e0\u8bba\u662f\u57fa\u56e0\u7ec4\u5b66\u3001\u8f6c\u5f55\u7ec4\u5b66\uff0c\u8fd8\u662f\u86cb\u767d\u8d28\u7ec4\u5b66\uff0c\u6570\u636e\u8d28\u91cf\u76f4\u63a5\u5f71\u54cd\u5206\u6790\u7ed3\u679c\u7684\u53ef\u9760\u6027\u548c\u51c6\u786e\u6027\u3002\u5728\u8fd9\u7bc7\u6587\u7ae0\u4e2d\uff0c\u6211\u4eec\u5c06\u63a2\u8ba8\u6570\u636e\u6e05\u6d17\u7684\u91cd\u8981\u6027\uff0c\u4ecb\u7ecd\u4e00\u4e9b\u5e38\u7528\u7684\u65b9\u6cd5\u548c\u5de5\u5177\uff0c\u5e76\u63ed\u793a\u4e00\u4e9b\u5e38\u89c1\u7684\u9677\u9631\u548c\u9519\u8bef\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">\u4e3a\u4ec0\u4e48\u6570\u636e\u6e05\u6d17\u5f88\u91cd\u8981\uff1f<\/h4>\n\n\n\n<ol>\n<li><strong>\u63d0\u9ad8\u6570\u636e\u8d28\u91cf<\/strong>\uff1a\u539f\u59cb\u751f\u7269\u6570\u636e\u901a\u5e38\u5305\u542b\u566a\u58f0\u548c\u9519\u8bef\uff0c\u5982\u6d4b\u5e8f\u9519\u8bef\u3001\u6837\u672c\u6c61\u67d3\u3001\u91cd\u590d\u6570\u636e\u7b49\u3002\u6570\u636e\u6e05\u6d17\u53ef\u4ee5\u53bb\u9664\u8fd9\u4e9b\u4e0d\u826f\u6570\u636e\uff0c\u63d0\u9ad8\u6570\u636e\u96c6\u7684\u6574\u4f53\u8d28\u91cf\u3002<\/li>\n\n\n\n<li><strong>\u786e\u4fdd\u5206\u6790\u7ed3\u679c\u7684\u53ef\u9760\u6027<\/strong>\uff1a\u672a\u7ecf\u6e05\u6d17\u7684\u6570\u636e\u53ef\u80fd\u5bfc\u81f4\u9519\u8bef\u7684\u5206\u6790\u7ed3\u679c\uff0c\u5f71\u54cd\u7814\u7a76\u7ed3\u8bba\u7684\u53ef\u9760\u6027\u3002\u4f8b\u5982\uff0c\u566a\u58f0\u6570\u636e\u53ef\u80fd\u5bfc\u81f4\u9519\u8bef\u7684\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\uff0c\u4ece\u800c\u8bef\u5bfc\u4e0b\u6e38\u5206\u6790\u3002<\/li>\n\n\n\n<li><strong>\u589e\u5f3a\u6570\u636e\u4e00\u81f4\u6027<\/strong>\uff1a\u751f\u7269\u6570\u636e\u901a\u5e38\u6765\u81ea\u591a\u4e2a\u6765\u6e90\u548c\u6279\u6b21\uff0c\u4e0d\u540c\u7684\u6279\u6b21\u53ef\u80fd\u5b58\u5728\u7cfb\u7edf\u8bef\u5dee\u3002\u6570\u636e\u6e05\u6d17\u53ef\u4ee5\u51cf\u5c11\u6279\u6b21\u6548\u5e94\uff0c\u63d0\u9ad8\u6570\u636e\u7684\u4e00\u81f4\u6027\u3002<\/li>\n\n\n\n<li><strong>\u8282\u7701\u8ba1\u7b97\u8d44\u6e90<\/strong>\uff1a\u6e05\u6d17\u540e\u7684\u6570\u636e\u4f53\u79ef\u66f4\u5c0f\uff0c\u5904\u7406\u901f\u5ea6\u66f4\u5feb\uff0c\u53ef\u4ee5\u663e\u8457\u51cf\u5c11\u8ba1\u7b97\u8d44\u6e90\u7684\u6d88\u8017\uff0c\u63d0\u9ad8\u5206\u6790\u6548\u7387\u3002<\/li>\n<\/ol>\n\n\n\n<h4 class=\"wp-block-heading\">\u6570\u636e\u6e05\u6d17\u7684\u65b9\u6cd5\u548c\u5de5\u5177<\/h4>\n\n\n\n<h5 class=\"wp-block-heading\">1. <strong>\u8d28\u91cf\u63a7\u5236<\/strong><\/h5>\n\n\n\n<p>\u8d28\u91cf\u63a7\u5236\u662f\u6570\u636e\u6e05\u6d17\u7684\u7b2c\u4e00\u6b65\uff0c\u5305\u62ec\u8bc4\u4f30\u548c\u8fc7\u6ee4\u4f4e\u8d28\u91cf\u6570\u636e\u3002<\/p>\n\n\n\n<p><strong>\u65b9\u6cd5<\/strong>\uff1a<\/p>\n\n\n\n<ul>\n<li><strong>\u53bb\u9664\u4f4e\u8d28\u91cf\u8bfb\u6570<\/strong>\uff1a\u8fc7\u6ee4\u6389\u6d4b\u5e8f\u8d28\u91cf\u4f4e\u7684\u8bfb\u6570\u6216\u78b1\u57fa\u3002<\/li>\n\n\n\n<li><strong>\u8bc4\u4f30\u6d4b\u5e8f\u6df1\u5ea6<\/strong>\uff1a\u786e\u4fdd\u6bcf\u4e2a\u6837\u672c\u7684\u6d4b\u5e8f\u6df1\u5ea6\u8db3\u591f\uff0c\u4ee5\u63d0\u4f9b\u53ef\u9760\u7684\u57fa\u56e0\u8868\u8fbe\u91cf\u3002<\/li>\n<\/ul>\n\n\n\n<p><strong>\u5de5\u5177<\/strong>\uff1a<\/p>\n\n\n\n<ul>\n<li><strong>FastQC<\/strong>\uff1a\u7528\u4e8e\u8bc4\u4f30\u6d4b\u5e8f\u6570\u636e\u8d28\u91cf\uff0c\u751f\u6210\u8be6\u7ec6\u7684\u8d28\u91cf\u62a5\u544a\u3002<\/li>\n\n\n\n<li><strong>Trimmomatic<\/strong>\uff1a\u7528\u4e8e\u53bb\u9664\u4f4e\u8d28\u91cf\u8bfb\u6570\u548c\u63a5\u5934\u5e8f\u5217\u3002<\/li>\n<\/ul>\n\n\n\n<h5 class=\"wp-block-heading\">2. <strong>\u53bb\u9664\u91cd\u590d\u6570\u636e<\/strong><\/h5>\n\n\n\n<p>\u91cd\u590d\u6570\u636e\u53ef\u80fd\u662f\u7531\u4e8ePCR\u6269\u589e\u7b49\u6280\u672f\u6b65\u9aa4\u9020\u6210\u7684\uff0c\u9700\u8981\u5728\u5206\u6790\u524d\u53bb\u9664\u3002<\/p>\n\n\n\n<p><strong>\u65b9\u6cd5<\/strong>\uff1a<\/p>\n\n\n\n<ul>\n<li><strong>\u6807\u8bb0\u548c\u53bb\u9664\u91cd\u590d\u8bfb\u6570<\/strong>\uff1a\u8bc6\u522b\u548c\u5220\u9664PCR\u6269\u589e\u4ea7\u751f\u7684\u91cd\u590d\u8bfb\u6570\uff0c\u4ee5\u51cf\u5c11\u504f\u5dee\u3002<\/li>\n<\/ul>\n\n\n\n<p><strong>\u5de5\u5177<\/strong>\uff1a<\/p>\n\n\n\n<ul>\n<li><strong>Picard<\/strong>\uff1a\u7528\u4e8e\u6807\u8bb0\u548c\u53bb\u9664\u91cd\u590d\u7684\u8bfb\u6570\u3002<\/li>\n\n\n\n<li><strong>SAMtools<\/strong>\uff1a\u63d0\u4f9b\u4e86\u4e00\u5957\u5904\u7406\u548c\u5206\u6790SAM\/BAM\u6587\u4ef6\u7684\u5de5\u5177\u3002<\/li>\n<\/ul>\n\n\n\n<h5 class=\"wp-block-heading\">3. <strong>\u53bb\u9664\u6279\u6b21\u6548\u5e94<\/strong><\/h5>\n\n\n\n<p>\u4e0d\u540c\u6279\u6b21\u7684\u5b9e\u9a8c\u53ef\u80fd\u5e26\u6765\u7cfb\u7edf\u8bef\u5dee\uff0c\u9700\u8981\u6821\u6b63\u4ee5\u63d0\u9ad8\u6570\u636e\u4e00\u81f4\u6027\u3002<\/p>\n\n\n\n<p><strong>\u65b9\u6cd5<\/strong>\uff1a<\/p>\n\n\n\n<ul>\n<li><strong>\u6279\u6b21\u6548\u5e94\u6821\u6b63<\/strong>\uff1a\u4f7f\u7528\u7edf\u8ba1\u65b9\u6cd5\u6821\u6b63\u4e0d\u540c\u6279\u6b21\u95f4\u7684\u7cfb\u7edf\u8bef\u5dee\u3002<\/li>\n<\/ul>\n\n\n\n<p><strong>\u5de5\u5177<\/strong>\uff1a<\/p>\n\n\n\n<ul>\n<li><strong>Combat<\/strong>\uff08\u5728SVA\u5305\u4e2d\uff09\uff1a\u7528\u4e8e\u6279\u6b21\u6548\u5e94\u6821\u6b63\u3002<\/li>\n\n\n\n<li><strong>Harman<\/strong>\uff1a\u53e6\u4e00\u79cd\u6279\u6b21\u6548\u5e94\u6821\u6b63\u5de5\u5177\u3002<\/li>\n<\/ul>\n\n\n\n<h5 class=\"wp-block-heading\">4. <strong>\u5f52\u4e00\u5316<\/strong><\/h5>\n\n\n\n<p>\u5f52\u4e00\u5316\u662f\u5c06\u6570\u636e\u6807\u51c6\u5316\uff0c\u4ee5\u4fbf\u4e0d\u540c\u6837\u672c\u4e4b\u95f4\u53ef\u4ee5\u8fdb\u884c\u6bd4\u8f83\u3002<\/p>\n\n\n\n<p><strong>\u65b9\u6cd5<\/strong>\uff1a<\/p>\n\n\n\n<ul>\n<li><strong>TPM\/RPKM\/FPKM<\/strong>\uff1a\u5e38\u7528\u7684\u8f6c\u5f55\u7ec4\u6570\u636e\u5f52\u4e00\u5316\u65b9\u6cd5\u3002<\/li>\n\n\n\n<li><strong>DESeq2<\/strong>\u548c<strong>EdgeR<\/strong>\uff1a\u7528\u4e8e\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u7684\u5f52\u4e00\u5316\u65b9\u6cd5\u3002<\/li>\n<\/ul>\n\n\n\n<p><strong>\u5de5\u5177<\/strong>\uff1a<\/p>\n\n\n\n<ul>\n<li><strong>DESeq2<\/strong>\uff1aR\u5305\uff0c\u7528\u4e8e\u5f52\u4e00\u5316\u548c\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u3002<\/li>\n\n\n\n<li><strong>EdgeR<\/strong>\uff1aR\u5305\uff0c\u7528\u4e8e\u5f52\u4e00\u5316\u548c\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u3002<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">\u5e38\u89c1\u7684\u9677\u9631\u548c\u9519\u8bef<\/h4>\n\n\n\n<ol>\n<li><strong>\u5ffd\u7565\u8d28\u91cf\u63a7\u5236<\/strong>\uff1a\u76f4\u63a5\u4f7f\u7528\u672a\u7ecf\u8d28\u91cf\u63a7\u5236\u7684\u6570\u636e\u8fdb\u884c\u5206\u6790\uff0c\u53ef\u80fd\u5bfc\u81f4\u9519\u8bef\u7684\u7ed3\u679c\u3002<\/li>\n\n\n\n<li><strong>\u4e0d\u8003\u8651\u6279\u6b21\u6548\u5e94<\/strong>\uff1a\u4e0d\u540c\u6279\u6b21\u7684\u7cfb\u7edf\u8bef\u5dee\u53ef\u80fd\u4e25\u91cd\u5f71\u54cd\u5206\u6790\u7ed3\u679c\uff0c\u4f46\u8bb8\u591a\u7814\u7a76\u5ffd\u89c6\u4e86\u8fd9\u4e00\u70b9\u3002<\/li>\n\n\n\n<li><strong>\u8fc7\u5ea6\u6e05\u6d17<\/strong>\uff1a\u8fc7\u5ea6\u6e05\u6d17\u53ef\u80fd\u5bfc\u81f4\u6570\u636e\u4e22\u5931\uff0c\u5f71\u54cd\u4e0b\u6e38\u5206\u6790\u7684\u654f\u611f\u6027\u3002<\/li>\n\n\n\n<li><strong>\u4f7f\u7528\u9519\u8bef\u7684\u5f52\u4e00\u5316\u65b9\u6cd5<\/strong>\uff1a\u4e0d\u540c\u6570\u636e\u7c7b\u578b\u9700\u8981\u4e0d\u540c\u7684\u5f52\u4e00\u5316\u65b9\u6cd5\uff0c\u4f7f\u7528\u4e0d\u5f53\u53ef\u80fd\u5bfc\u81f4\u8bef\u5bfc\u6027\u7ed3\u679c\u3002<\/li>\n\n\n\n<li><strong>\u7f3a\u4e4f\u8bb0\u5f55\u548c\u518d\u73b0\u6027<\/strong>\uff1a\u672a\u80fd\u8be6\u7ec6\u8bb0\u5f55\u6570\u636e\u6e05\u6d17\u6b65\u9aa4\u548c\u53c2\u6570\uff0c\u5bfc\u81f4\u5206\u6790\u7ed3\u679c\u96be\u4ee5\u518d\u73b0\u3002<\/li>\n<\/ol>\n\n\n\n<h4 class=\"wp-block-heading\">\u7ed3\u8bba<\/h4>\n\n\n\n<p>\u6570\u636e\u6e05\u6d17\u5728\u751f\u7269\u4fe1\u606f\u5b66\u7814\u7a76\u4e2d\u81f3\u5173\u91cd\u8981\uff0c\u5b83\u76f4\u63a5\u5f71\u54cd\u5206\u6790\u7ed3\u679c\u7684\u8d28\u91cf\u548c\u53ef\u9760\u6027\u3002\u901a\u8fc7\u6b63\u786e\u7684\u65b9\u6cd5\u548c\u5de5\u5177\uff0c\u53ef\u4ee5\u663e\u8457\u63d0\u9ad8\u6570\u636e\u7684\u4e00\u81f4\u6027\u548c\u51c6\u786e\u6027\u3002\u7136\u800c\uff0c\u5728\u6570\u636e\u6e05\u6d17\u8fc7\u7a0b\u4e2d\uff0c\u7814\u7a76\u4eba\u5458\u9700\u8981\u8b66\u60d5\u5e38\u89c1\u7684\u9677\u9631\u548c\u9519\u8bef\uff0c\u786e\u4fdd\u6e05\u6d17\u6b65\u9aa4\u79d1\u5b66\u5408\u7406\uff0c\u8bb0\u5f55\u8be6\u5c3d\uff0c\u4ee5\u5b9e\u73b0\u9ad8\u8d28\u91cf\u7684\u751f\u7269\u4fe1\u606f\u5b66\u7814\u7a76\u3002<\/p>\n\n\n\n<p>\u5e0c\u671b\u8fd9\u7bc7\u6587\u7ae0\u80fd\u591f\u5e2e\u52a9\u4f60\u7406\u89e3\u6570\u636e\u6e05\u6d17\u7684\u91cd\u8981\u6027\uff0c\u5e76\u4e3a\u4f60\u7684\u751f\u7269\u4fe1\u606f\u5b66\u7814\u7a76\u63d0\u4f9b\u6709\u4ef7\u503c\u7684\u53c2\u8003\u3002\u5982\u679c\u6709\u4efb\u4f55\u95ee\u9898\u6216\u9700\u8981\u8fdb\u4e00\u6b65\u7684\u6307\u5bfc\uff0c\u6b22\u8fce\u968f\u65f6\u8054\u7cfb\u3002<\/p>\n\n\n\n<p>\u63d0\u4f9b\u670d\u52a1\u5668\u7684\u8bd5\u7528\u3002<a href=\"http:\/\/www.biocloudservice.com\/wordpress\/?p=40003\" target=\"_blank\" rel=\"noreferrer noopener\">\u8bf7\u8054\u7cfb\u6211\u4eec\u3002<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u6570\u636e\u6e05\u6d17\u662f\u751f\u7269\u4fe1\u606f\u5b66\u7814\u7a76\u4e2d\u4e0d\u53ef\u6216\u7f3a\u7684\u4e00\u6b65\u3002\u65e0\u8bba\u662f\u57fa\u56e0\u7ec4\u5b66\u3001\u8f6c\u5f55\u7ec4\u5b66\uff0c\u8fd8\u662f\u86cb\u767d\u8d28\u7ec4\u5b66\uff0c\u6570\u636e\u8d28\u91cf\u76f4\u63a5\u5f71\u54cd\u5206\u6790\u7ed3\u679c\u7684 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