{"id":43297,"date":"2024-06-02T01:15:25","date_gmt":"2024-06-01T17:15:25","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=43297"},"modified":"2024-06-11T10:32:12","modified_gmt":"2024-06-11T02:32:12","slug":"9aa474f7fa","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=43297","title":{"rendered":"\u590d\u65e6\u5927\u5b66\u9644\u5c5e\u533b\u9662\u56e2\u961f\u51fa\u5947\u62db\u2014\u2014\u5355\u7ec6\u80deRNA\u6d4b\u5e8f+\u7a7a\u95f4\u8f6c\u5f55\u7ec4+\u6df1\u5ea6\u5b66\u4e60\uff0c\u5206\u5206\u949f\u641e\u5b9a7\u5206+\u6587\u7ae0\uff0c\u62fc\u624b\u901f\u7684\u65f6\u5019\u5230\u4e86\uff01"},"content":{"rendered":"
\n

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\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u662f\u5bf9\u7279\u5b9a\u7ec6\u80de\u7fa4\u4f53\u8fdb\u884c\u57fa\u56e0\u8868\u8fbe\u8c31\u5b9a\u91cf\u7684\u9ad8\u901a\u91cf\u5b9e\u9a8c\u6280\u672f\uff0c\u7136\u800c\u7ec6\u80de\u7684\u7a7a\u95f4\u6392\u5217\u5e38\u5e38\u4f1a\u4e22\u5931\u3002\u901a\u8fc7\u5206\u6790\u5df2\u77e5\u7684\u53c2\u8003\u56fe\u8c31\uff0c\u5c06\u7a7a\u95f4\u4fe1\u606f\u5f52\u96c6\u5230scRNA-seq\u6570\u636e\u4e0a\u3002\u4f46\u662f\u4e5f\u7f3a\u4e4f\u5bf9\u6620\u5c04\u7684\u51c6\u786e\u6027\u548c\u7cbe\u786e\u6027\uff0c\u968f\u7740\u53d1\u5c55\uff0c\u57fa\u4e8e\u6df1\u5ea6\u5b66\u4e60\u7684\u65b9\u6cd5\u63d0\u4f9b\u4e86\u66f4\u591a\u53ef\u80fd\u6027\uff01<\/span><\/p>\n

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\u6742\u5fd7\uff1aJournal of Translational Medicine<\/span><\/section>\n
\u5f71\u54cd\u56e0\u5b50\uff1aIF=7.4<\/span><\/section>\n

\u53d1\u8868\u65f6\u95f4\uff1a2024\u5e742\u6708<\/span><\/p>\n

<\/mp-common-videosnap><\/section>\n

\u7814\u7a76\u80cc\u666f<\/span><\/p>\n

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\u6570\u636e\u6765\u6e90<\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n
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\u6570\u636e\u96c6\/\u961f\u5217<\/span><\/p>\n<\/td>\n

\n

\u6570\u636e\u5e93<\/span><\/p>\n<\/td>\n

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\u6570\u636e\u7c7b\u578b<\/span><\/p>\n<\/td>\n

\n

\u8be6\u7ec6\u4fe1\u606f<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

GSE202374<\/span><\/p>\n<\/td>\n

\n

GEO<\/span><\/p>\n<\/td>\n

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RNA-Seq\u6570\u636e<\/span><\/p>\n<\/td>\n

\n

76\u540d\u60a3\u8005\u7684195\u4efd\u6837\u672c<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

GSE224630<\/span><\/p>\n<\/td>\n

\n

GEO<\/span><\/p>\n<\/td>\n

\n

RNA-Seq\u6570\u636e<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

GSE207493<\/span><\/p>\n<\/td>\n

\n

GEO<\/span><\/p>\n<\/td>\n

\n

RNA-Seq\u6570\u636e<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

GSE121638<\/span><\/p>\n<\/td>\n

\n

GEO<\/span><\/p>\n<\/td>\n

\n

RNA-Seq\u6570\u636e<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

GSE159115<\/span>        <\/o:page><\/p>\n<\/td>\n

\n

GEO<\/span><\/p>\n<\/td>\n

\n

RNA-Seq\u6570\u636e<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

GSE175540<\/span><\/p>\n<\/td>\n

\n

GEO<\/span><\/p>\n<\/td>\n

\n

RNA-Seq\u6570\u636e<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u5176\u4ed6\u53c2\u8003\u6587\u732e<\/span><\/p>\n<\/td>\n

\n

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\n

RNA-Seq\u6570\u636e<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6570\u636e\u601d\u8def<\/span><\/p>\n

\u4f5c\u8005\u6784\u5efa\u4e86\u4e00\u4e2a\u5168\u9762\u3001\u9ad8\u5206\u8fa8\u7387\u7684ccRCC\u5355\u7ec6\u80de\u56fe\u8c31\uff0c\u4ee5\u63a2\u7d22\u80bf\u7624\u5fae\u73af\u5883\u4e2d\u7684\u4ee3\u8c22\u91cd\u7f16\u7a0b\uff0c\u8fd8\u7ed3\u5408\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\u6765\u8fdb\u4e00\u6b65\u5256\u6790\u7a7a\u95f4\u4f4d\u7f6e\u7684\u4ee3\u8c22\u5f02\u8d28\u6027\uff0c\u89c2\u5bdf\u5230\u5404\u79cd\u7ec6\u80de\u7c7b\u578b\u7684\u4ee3\u8c22\u91cd\u7f16\u7a0b\u73b0\u8c61\u548c\u76f8\u5173\u751f\u7269\u5b66\u7279\u5f81\uff0c\u5305\u62ecCD8<\/span>+<\/sup><\/span>T\u7ec6\u80de\u8fdb\u5316\u4e2d\u80fd\u91cf\u4ee3\u8c22\u7684\u5f02\u8d28\u6027\u4ee5\u53ca\u5de8\u566c\u7ec6\u80de\u9798\u8102\u4ee3\u8c22\u4e0eM2\u6781\u5316\u4e4b\u95f4\u7684\u5f3a\u76f8\u5173\u6027\u3002\u4f5c\u8005\u5c06\u4ee3\u8c22\u7269\u7684\u7a7a\u95f4\u5206\u5e03\u5206\u4e3a\u79bb\u6563\u578b\u3001\u5916\u56f4\u578b\u548c\u4e2d\u5fc3\u578b\u3002\u6b64\u5916\uff0c\u4f5c\u8005\u8fd8\u5f00\u53d1\u4e86scMet \u8f6f\u4ef6\u5305\uff0c\u8fd9\u662f\u4e00\u4e2a\u80fd\u591f\u5c06RNA-seq \u6570\u636e\u53cd\u5377\u79ef\u4e3ascRNA-seq\u6570\u636e\u7684 Python\u7a0b\u5e8f\uff0c\u65e8\u5728\u4fc3\u8fdb\u5355\u7ec6\u80de\u6d4b\u5e8f\u7684\u4e34\u5e8a\u5e94\u7528\u3002\u9009\u53d6\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\u8d44\u6599\u8fdb\u884c\u7a7a\u95f4\u5b9a\u4f4d\u4ee3\u8c22\u6d3b\u6027\u5206\u6790\u3002<\/span><\/p>\n

\u7814\u7a76\u7ed3\u679c<\/span><\/p>\n

1.\u900f\u660e\u7ec6\u80de\u80be\u7ec6\u80de\u764c\u7684\u80bf\u7624\u5fae\u73af\u5883\u5f02\u8d28\u6027\u5206\u6790\u63ed\u793a\u4e86\u4e0d\u540c\u7684\u7ec6\u80de\u6d78\u6da6\u6a21\u5f0f<\/span><\/p>\n

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5.<\/span> <\/span>ENPP2\u4f5c\u4e3a\u6f5c\u5728\u7684\u9884\u540e\u6807\u5fd7\u7269\u548c\u80bf\u7624\u6e90\u6027\u5185\u76ae\u7ec6\u80de\u6307\u6807<\/span><\/p>\n

\u4f5c\u8005\u5206\u79bb\u5185\u76ae\u7ec6\u80de\u5e76\u5c06\u5176\u5206\u4e3a\u4e0d\u540c\u7684\u4e9a\u578b\uff08\u56fe5A)\uff0c\u5728\u4e0d\u540c\u7ec4\u7ec7\u5b66\u7ea7\u522b\u4e2d\uff0c\u6ce8\u610f\u5230\u4f4e\u7ea7\u522b\u80bf\u7624\u4e2d\u4e3b\u8981\u5bcc\u96c6CHRBP3<\/span>+<\/sup><\/span>\u6bdb\u7ec6\u8840\u7ba1\u5185\u76ae\u7ec6\u80de(\u56fe5B)\u3002\u5229\u7528CHRBP3<\/span>+<\/sup><\/span>\u6bdb\u7ec6\u8840\u7ba1\u5185\u76ae\u7ec6\u80de\u7279\u5f02\u6027\u57fa\u56e0\u96c6\u7684\u5bcc\u96c6\u6c34\u5e73\u4f5c\u4e3a\u751f\u5b58\u9884\u540e\u5206\u6790\u5206\u7ec4\u7684\u57fa\u7840\uff0c\u8be5\u5206\u6790\u663e\u793a\uff0c\u80bf\u7624\u5185\u8be5\u57fa\u56e0\u7ec4\u8868\u8fbe\u5347\u9ad8\u7684\u60a3\u8005\u603b\u4f53\u751f\u5b58\u7387\u663e\u8457\u63d0\u9ad8(\u56fe5C)\uff0c\u6697\u793a\u4e86CHRBP3<\/span>+<\/sup><\/span>\u6bdb\u7ec6\u8840\u7ba1\u5185\u76ae\u7ec6\u80de\u4e0e\u826f\u597d\u9884\u540e\u4e4b\u95f4\u7684\u6f5c\u5728\u5173\u8054\u3002\u4f5c\u8005\u5bf9\u4e0d\u540c\u6765\u6e90\u7684\u5185\u76ae\u7ec6\u80de\u8fdb\u884c\u4e86\u5dee\u5f02\u5206\u6790\uff0c\u53d1\u73b0\u52a8\u8109\u5185\u76ae\u7ec6\u80de\u548c\u6bdb\u7ec6\u8840\u7ba1\u5185\u76ae\u7ec6\u80de\u8868\u73b0\u51fa\u60ca\u4eba\u7684\u76f8\u4f3c\u5dee\u5f02(\u56fe5D)\uff0cENPP2\u4e3b\u8981\u5728\u5185\u76ae\u7ec6\u80de\u4e2d\u8868\u8fbe\uff0c\u800c\u4e0d\u662f\u5728\u5176\u4ed6\u7ec6\u80de\u7c7b\u578b\u4e2d\u8868\u8fbe(\u56fe5E)\u3002\u57fa\u4e8eENPP2\u8868\u8fbe\u7684\u751f\u5b58\u9884\u540e\u5206\u6790\u663e\u793a\uff0c\u8be5\u5185\u76ae\u6807\u5fd7\u7269\u8868\u8fbe\u6c34\u5e73\u8f83\u9ad8\u7684\u60a3\u8005\u9884\u540e\u8f83\u597d(\u56fe5F)\u3002\u56e0\u6b64\uff0cENPP2\u53ef\u4ee5\u4f5c\u4e3a\u80bf\u7624\u6e90\u6027\u5185\u76ae\u7ec6\u80de\u7684\u7279\u5f02\u6027\u751f\u7269\u6807\u5fd7\u7269\u548c\u9884\u540e\u6307\u6807<\/span><\/p>\n

\"\u590d\u65e6\u5927\u5b66\u9644\u5c5e\u533b\u9662\u56e2\u961f\u51fa\u5947\u62db\u2014\u2014\u5355\u7ec6\u80deRNA\u6d4b\u5e8f+\u7a7a\u95f4\u8f6c\u5f55\u7ec4+\u6df1\u5ea6\u5b66\u4e60\uff0c\u5206\u5206\u949f\u641e\u5b9a7\u5206+\u6587\u7ae0\uff0c\u62fc\u624b\u901f\u7684\u65f6\u5019\u5230\u4e86\uff01\"<\/span><\/span><\/p>\n

\u56fe5 \u5de8\u566c\u5185\u76ae\u7ec6\u80de\u8868\u8fbe\u5f02\u8d28\u6027<\/span><\/span><\/p>\n

6.\u7ed8\u5236ccRCC\u5fae\u73af\u5883\u4e2d\u7684\u7a7a\u95f4\u4ee3\u8c22\u6d3b\u6027\u56fe\u8c31<\/span><\/p>\n

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AMP\u3001CDP\u548c\u8102\u80aa\u9178\u5728\u80bf\u7624\u533a\u57df\u8868\u73b0\u51fa\u660e\u663e\u7684\u5bcc\u96c6\u503e\u5411\uff0c\u5448\u201c\u4e2d\u5fc3\u201d\u5206\u5e03\u6a21\u5f0f\uff0c\u76f8\u53cd\u4e59\u9170\u8f85\u9176a\u3001\u9e1f\u6c28\u9178\u548c\u916a\u6c28\u9178\u5728\u80bf\u7624\u5305\u819c\u7684\u8fd1\u5468\u8868\u73b0\u51fa\u9ad8\u5ea6\u5bcc\u96c6\u7684\u503e\u5411\uff0c\u8868\u73b0\u51fa\u72ec\u7279\u7684\u201c\u5468\u56f4\u201d\u5206\u5e03\u3002\u5c3d\u7ba1\u7425\u73c0\u9178\u76d0\u3001\u8c37\u6c28\u9178\u76d0\u548c\u5bcc\u9a6c\u9178\u76d0\u5728\u80bf\u7624\u533a\u57df\u5bcc\u96c6\uff0c\u4f46\u5b83\u4eec\u7684\u5206\u5e03\u5e76\u6ca1\u6709\u5448\u73b0\u8fde\u7eed\u7684\u8f68\u8ff9\uff0c\u800c\u662f\u91c7\u7528\u4e86\u53ef\u8bc6\u522b\u7684\u201c\u79bb\u6563\u201d\u5206\u5e03(\u56fe6F-H)\u3002<\/span><\/p>\n

\"\u590d\u65e6\u5927\u5b66\u9644\u5c5e\u533b\u9662\u56e2\u961f\u51fa\u5947\u62db\u2014\u2014\u5355\u7ec6\u80deRNA\u6d4b\u5e8f+\u7a7a\u95f4\u8f6c\u5f55\u7ec4+\u6df1\u5ea6\u5b66\u4e60\uff0c\u5206\u5206\u949f\u641e\u5b9a7\u5206+\u6587\u7ae0\uff0c\u62fc\u624b\u901f\u7684\u65f6\u5019\u5230\u4e86\uff01\"<\/span><\/span><\/p>\n

\u56fe6\u7a7a\u95f4\u4ee3\u8c22\u6d3b\u52a8\u7684\u5f02\u8d28\u6027<\/span><\/p>\n

7.<\/span> <\/span>scMet\uff1a\u4e00\u79cd\u57fa\u4e8e\u6df1\u5ea6\u5b66\u4e60\u7684\u65b9\u6cd5\uff0c\u7528\u4e8e\u52a0\u901fscRNA-seq\u5728\u764c\u75c7\u4e2d\u7684\u4e34\u5e8a\u5e94\u7528<\/span>    <\/o:page><\/p>\n

\u4f5c\u8005\u5f15\u5165\u4e86scMet\uff0c\u8fd9\u662f\u4e00\u79cd\u5229\u7528\u6df1\u5ea6\u5b66\u4e60\u5e73\u53f0(\u6761\u4ef6\u53d8\u5206\u81ea\u7f16\u7801\u5668)\u7684\u521b\u65b0\u89e3\u51b3\u65b9\u6848\uff0c\u5176\u4e3b\u8981\u76ee\u6807\u662f\u5c06\u5927\u91cfRNA\u6d4b\u5e8f\u6570\u636e\u8f6c\u5316\u4e3ascRNA -seq\u8c31(\u56fe7A)\uff0c\u8fd9\u4e00\u52aa\u529b\u65e8\u5728\u901a\u8fc7\u51cf\u8f7b\u8fd9\u4e9b\u6311\u6218\u6765\u7b80\u5316scRNA-seq\u7684\u4e34\u5e8a\u5e94\u7528\u3002\u5229\u7528scMet\u5c06TCGA\u7684RNAseq\u6570\u636e\u8f6c\u5316\u4e3a\u5305\u542b5401964\u4e2a\u7ec6\u80de\u548c3000\u4e2a\u4ee3\u8c22\u76f8\u5173\u57fa\u56e0\u7684scRNA-seq\u6570\u636e\u77e9\u9635\uff0c\u5f97\u5230\u8d85\u8fc7150\u4ebf\u4e2aRNA\u8868\u8fbe\u503c(\u56fe7B)\u3002\u62df\u5408\u540e\uff0c\u751f\u6210\u7684UMAP\u56fe\u8868\u660e\uff0cscRNA-seq\u6570\u636e\u53ef\u4ee5\u66ff\u4ee3\u4e0b\u6e38\u5206\u6790\u4e2d\u7684RNA-seq\u6570\u636e\uff0c\u663e\u793a\u51fa\u5f3a\u5927\u7684\u7ec6\u80de\u805a\u7c7b\u6027\u80fd\u3002<\/span><\/p>\n

\"\u590d\u65e6\u5927\u5b66\u9644\u5c5e\u533b\u9662\u56e2\u961f\u51fa\u5947\u62db\u2014\u2014\u5355\u7ec6\u80deRNA\u6d4b\u5e8f+\u7a7a\u95f4\u8f6c\u5f55\u7ec4+\u6df1\u5ea6\u5b66\u4e60\uff0c\u5206\u5206\u949f\u641e\u5b9a7\u5206+\u6587\u7ae0\uff0c\u62fc\u624b\u901f\u7684\u65f6\u5019\u5230\u4e86\uff01\"<\/span><\/span><\/p>\n

\u56fe7 scMet\u5de5\u4f5c\u6d41\u7a0b\u548cTCGA RNA-seq\u6570\u636e\u7684\u8f6c\u6362\u3002<\/span>    <\/o:page><\/p>\n

\u6587\u7ae0\u5c0f\u7ed3<\/span><\/p>\n

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\u5927\u5bb6\u6216\u8bb8\u4e86\u89e3\u5355\u7ec6\u80de\u6d4b\u5e8f\u6280\u672f\uff0c\u5355\u7ec6\u80de\u591a\u7ec4\u5b66\u3001\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\u5206\u522b\u662f\u5404\u8457\u540d\u671f\u520a\u57282019\u5e74\u548c2020\u5e74\u7684\u5e74\u5ea6\u6280\u672f\uff0c\u4e0d\u8bba\u8fc7\u53bb\u8fd8\u662f\u672a\u6765\uff0c\u5b83\u4eec\u5bf9\u4e8e\u751f\u547d\u79d1\u5b66\u80fd\u591f\u5e26\u6765\u5f88\u591a\u65b0\u7684\u52a9\u63a8\u4f5c\u7528\u3002\u6df1\u5ea6\u5b66\u4e60+\u5355\u7ec6\u80deRNA\u6d4b\u5e8f+\u7a7a\u95f4\u8f6c\u5f55\u7ec4\uff0c\u6280\u672f\u4e0e\u6311\u6218\u5e76\u5b58\uff0c\u4f46\u7814\u7a76\u8d8a\u6df1\u5165\uff0cpaper\u8d8a\u9ad8\u5206\uff0c<\/span>\u60f3\u5b66\u4e60\u5927\u4f6c\u4eec\u8fdb\u884c\u7c7b\u4f3c\u7814\u7a76\u7684\uff0c\u53ef\u4ee5\u6765\u627e\u5c0f\u4e91\u54e6\uff0c\u5e26\u4f60\u8d70\u5728\u70ed\u70b9\u524d\u6cbf\uff0c\u5b66\u4f1a\u6574\u5957\u7ec4\u5408\u6280\u80fd\uff01\u6709\u9700\u6c42\u76f4\u63a5\u8054\u7cfb\u5566~<\/span>  <\/span><\/p>\n

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\u5b9a\u5236\u751f\u4fe1\u5206\u6790<\/span><\/strong><\/span><\/p>\n

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\u4e91\u670d\u52a1\u5668\u79df\u8d41<\/strong><\/span>\n<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u70ed\u70b9\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u5b5f\u5fb7\u5c14\u968f\u673a\u5316<\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n
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\u4e34\u5e8a\u516c\u5171\u6570\u636e\u5206\u6790<\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n
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\u5355\u7ec6\u80de\u6d4b\u5e8f<\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n
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\u80bf\u7624\u514d\u75ab\u4e0e\u5fae\u73af\u5883<\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n
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\u673a\u5668\u5b66\u4e60<\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n
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\u5355\u57fa\u56e0\u5206\u6790<\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n
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\u751f\u4fe1\u4e91\u670d\u52a1\u5668<\/strong><\/span><\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u4ee3\u7801\u5408\u96c6<\/strong><\/span><\/a>\uff08\u70b9\u51fb\u67e5\u770b\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u57f9\u8bad\u73ed\u7cfb\u5217<\/span><\/strong>\uff08\u70b9\u51fb\u67e5\u770b\uff09<\/span><\/span><\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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