{"id":43500,"date":"2024-06-02T01:17:03","date_gmt":"2024-06-01T17:17:03","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=43500"},"modified":"2024-06-10T09:16:17","modified_gmt":"2024-06-10T01:16:17","slug":"935281ea60","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=43500","title":{"rendered":"1\u533a10\u5206+\u9884\u540e\u6587\u7ae0\u6709\u4f55\u4e7e\u5764\uff1f\u534e\u897f\u533b\u9662\u56e2\u961f\uff1a\u8870\u8001\u7279\u5f81\u57fa\u56e0+\u591a\u7ec4\u5b66\u5206\u6790\uff0c\u7ecf\u5178\u601d\u8def\u89e3\u9501\u9690\u85cf\u65b0\u73a9\u6cd5\uff01\uff01"},"content":{"rendered":"
\n

\"1\u533a10\u5206+\u9884\u540e\u6587\u7ae0\u6709\u4f55\u4e7e\u5764\uff1f\u534e\u897f\u533b\u9662\u56e2\u961f\uff1a\u8870\u8001\u7279\u5f81\u57fa\u56e0+\u591a\u7ec4\u5b66\u5206\u6790\uff0c\u7ecf\u5178\u601d\u8def\u89e3\u9501\u9690\u85cf\u65b0\u73a9\u6cd5\uff01\uff01\"<\/p>\n

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\n
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\n
\n
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\n
\"1\u533a10\u5206+\u9884\u540e\u6587\u7ae0\u6709\u4f55\u4e7e\u5764\uff1f\u534e\u897f\u533b\u9662\u56e2\u961f\uff1a\u8870\u8001\u7279\u5f81\u57fa\u56e0+\u591a\u7ec4\u5b66\u5206\u6790\uff0c\u7ecf\u5178\u601d\u8def\u89e3\u9501\u9690\u85cf\u65b0\u73a9\u6cd5\uff01\uff01\"<\/section>\n<\/section>\n<\/section>\n
\n
\n
\u5b9a\u5236\u751f\u4fe1\u5206\u6790<\/strong><\/span><\/p>\n

\u4e91\u670d\u52a1\u5668\u79df\u8d41<\/strong><\/span><\/p>\n

(\u52a0\u5fae\u4fe1\u5907\u6ce899<\/span>\u9886\u53d6\u8bd5\u7528)<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

  <\/o:p><\/span>    <\/span><\/span><\/p>\n

\"1\u533a10\u5206+\u9884\u540e\u6587\u7ae0\u6709\u4f55\u4e7e\u5764\uff1f\u534e\u897f\u533b\u9662\u56e2\u961f\uff1a\u8870\u8001\u7279\u5f81\u57fa\u56e0+\u591a\u7ec4\u5b66\u5206\u6790\uff0c\u7ecf\u5178\u601d\u8def\u89e3\u9501\u9690\u85cf\u65b0\u73a9\u6cd5\uff01\uff01\"<\/span><\/p>\n

\u25cf \u9898\u76ee\uff1a\u8870\u8001\u76f8\u5173\u57fa\u56e0\u7684\u6574\u5408\u5206\u6790\u786e\u5b9a\u4e86\u809d\u7ec6\u80de\u764c\u4e2d\u5177\u6709\u72ec\u7279\u7279\u5f81\u7684\u7a33\u5065\u9884\u540e\u4e9a\u578b<\/span><\/p>\n

\u25cf \u6742\u5fd7\uff1a<\/span>Journal of Advanced Research<\/span><\/span><\/p>\n

\u25cf \u5f71\u54cd\u56e0\u5b50\uff1aIF=10.7<\/span><\/p>\n

\u25cf \u53d1\u8868\u65f6\u95f4\uff1a2024\u5e744\u6708<\/span><\/p>\n

\u7814\u7a76\u80cc\u666f<\/span><\/p>\n

\u8870\u8001\u662f\u6307\u7ec6\u80de\u751f\u957f\u6c38\u4e45\u505c\u6b62\u7684\u4e00\u79cd\u72b6\u6001\uff0c\u88ab\u8ba4\u4e3a\u662f\u4e00\u79cd\u80bf\u7624\u6291\u5236\u673a\u5236\uff0c\u800c\u8d8a\u6765\u8d8a\u591a\u7684\u8bc1\u636e\u8868\u660e\uff0c\u8870\u8001\u7ec6\u80de\u5728\u764c\u75c7\u8fdb\u5c55\u8fc7\u7a0b\u4e2d\u8d77\u7740\u4e0d\u5229\u4f5c\u7528\u3002\u7531\u4e8e\u7f3a\u4e4f\u53cd\u6620\u764c\u75c7\u8870\u8001\u6c34\u5e73\u7684\u7279\u5f02\u800c\u53ef\u9760\u7684\u6807\u5fd7\u7269\uff0c\u8fd9\u963b\u788d\u4e86\u6211\u4eec\u5bf9\u8fd9\u4e00\u751f\u7269\u5b66\u57fa\u7840\u7684\u7406\u89e3\u3002<\/span><\/p>\n

\u6570\u636e\u6765\u6e90<\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
\n

\u6570\u636e\u96c6\/\u961f\u5217<\/span><\/p>\n<\/td>\n

\n

\u6570\u636e\u5e93<\/span><\/p>\n<\/td>\n

\n

\u6570\u636e\u7c7b\u578b<\/span><\/p>\n<\/td>\n

\n

\u8be6\u7ec6\u4fe1\u606f<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

LIHC<\/span><\/p>\n<\/td>\n

\n

UCSC Xena<\/span><\/p>\n<\/td>\n

\n

\u8868\u8fbe\u77e9\u9635\u548c\u4e34\u5e8a\u4fe1\u606f<\/span><\/p>\n<\/td>\n

\n

424\u4f8b\u809d\u764c\u6837\u672c<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

GDC<\/span><\/p>\n<\/td>\n

\n

\u57fa\u56e0\u7ec4\u7a81\u53d8\u6570\u636e<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

LIRI<\/span><\/p>\n<\/td>\n

\n

ICGC<\/span><\/p>\n<\/td>\n

\n

mRNA\u8868\u8fbe\u8c31\u548c\u4e34\u5e8a\u4fe1\u606f<\/span><\/p>\n<\/td>\n

\n

377\u4f8b\u809d\u764c\u6837\u672c<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

GSE14520<\/span><\/p>\n<\/td>\n

\n

GEO<\/span><\/p>\n<\/td>\n

\n

\u5fae\u9635\u5217\u6570\u636e<\/span><\/p>\n<\/td>\n

\n

42 \u4e2a\u80bf\u7624\u6837\u672c\u548c\u914d\u5bf9\u7684\u975e\u80bf\u7624\u6837\u672c<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

GSE54236<\/span>        <\/span><\/o:page><\/p>\n<\/td>\n

\n

GEO<\/span><\/p>\n<\/td>\n

\n

\u5fae\u9635\u5217\u6570\u636e<\/span><\/p>\n<\/td>\n

\n

161 \u4e2a\u5fae\u9635\u5217\u6570\u636e\u96c6<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

GSE125449<\/span><\/p>\n<\/td>\n

\n

GEO<\/span><\/p>\n<\/td>\n

\n

scRNA-seq\u6570\u636e<\/span><\/p>\n<\/td>\n

\n

9 \u4f8b\u809d\u7ec6\u80de\u764c\u548c 10 \u4f8b\u809d\u5185\u80c6\u7ba1\u764c\u60a3\u8005<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

GSE155182<\/span><\/p>\n<\/td>\n

\n

GEO<\/span><\/p>\n<\/td>\n

\n

scRNA-seq\u6570\u636e<\/span><\/p>\n<\/td>\n

\n

\u809d\u810f\u6b63\u5e38\u6216\u8bf1\u53d1\u7684\u975e\u9152\u7cbe\u6027\u8102\u80aa\u6027\u809d\u708e\u5c0f\u9f20\u6a21\u578b<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

IMvigor210<\/span><\/p>\n<\/td>\n

\n

IMvigor210<\/span><\/p>\n<\/td>\n

\n

RNA-seq\u6570\u636e<\/span><\/p>\n<\/td>\n

\n

PD-L1\u6cbb\u7597\u7684\u5c3f\u8def\u4e0a\u76ae\u764c\u6570\u636e<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

GSE78220<\/span><\/p>\n<\/td>\n

\n

GEO<\/span><\/p>\n<\/td>\n

\n

RNA-seq\u6570\u636e<\/span><\/p>\n<\/td>\n

\n

PD-1\u6cbb\u7597\u9ed1\u8272\u7d20\u7624\u6570\u636e<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

          <\/o:p><\/span><\/p>\n

\u7814\u7a76\u601d\u8def<\/span><\/p>\n

\u6536\u96c6SRGs\u8fdb\u884c\u7efc\u5408\u5206\u6790\uff0c\u4ee5\u63ed\u793a\u8870\u8001\u5728HCC\u4e2d\u7684\u4f5c\u7528\u3002\u6839\u636e SRGs \u5bf9 HCC \u8fdb\u884c\u5171\u8bc6\u805a\u7c7b\u3002\u91c7\u7528\u4e86\u591a\u79cd\u8ba1\u7b97\u65b9\u6cd5\uff0c\u5305\u62ec\u5355\u6837\u672c\u57fa\u56e0\u7ec4\u5bcc\u96c6\u5206\u6790\uff08ssGSEA\uff09\u548c\u6a21\u7cca c-means \u7b97\u6cd5\u3002\u5229\u7528\u836f\u7269\u654f\u611f\u6027\u6570\u636e\u7b5b\u9009\u51fa\u9488\u5bf9\u4e0d\u540c\u8870\u8001\u60a3\u8005\u7684\u6f5c\u5728\u6cbb\u7597\u836f\u7269\u3002\u6b64\u5916\uff0c\u8fd8\u5f00\u53d1\u4e86\u4e00\u79cd\u79f0\u4e3a\u7279\u5f81\u76f8\u5173\u57fa\u56e0\u5206\u6790\uff08SRGA\uff09\u7684\u65b9\u6cd5\uff0c\u7528\u4e8e\u8bc6\u522b\u4e0e\u76f8\u5173\u8868\u578b\u76f8\u5173\u7684\u6807\u8bb0\u7269\u3002\u5b9e\u9a8c\u7b56\u7565\u5305\u62ec\u5b9a\u91cf\u5b9e\u65f6 PCR\uff08qRT-PCR\uff09\u3001\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\u6d4b\u5b9a\u3001Western \u5370\u8ff9\u548c\u80bf\u7624-T \u7ec6\u80de\u5171\u57f9\u517b\u7cfb\u7edf\uff0c\u7528\u4e8e\u5728\u4f53\u5916\u9a8c\u8bc1\u7814\u7a76\u7ed3\u679c\u3002<\/span><\/p>\n

\"1\u533a10\u5206+\u9884\u540e\u6587\u7ae0\u6709\u4f55\u4e7e\u5764\uff1f\u534e\u897f\u533b\u9662\u56e2\u961f\uff1a\u8870\u8001\u7279\u5f81\u57fa\u56e0+\u591a\u7ec4\u5b66\u5206\u6790\uff0c\u7ecf\u5178\u601d\u8def\u89e3\u9501\u9690\u85cf\u65b0\u73a9\u6cd5\uff01\uff01\"<\/span><\/p>\n

<\/o:p><\/span><\/p>\n

\u4e3b\u8981\u7ed3\u679c<\/span><\/p>\n

1. SRGs\u7684\u8f6c\u5f55\u7ec4\u56fe\u8c31\u5b9a\u4e49\u4e86\u4e09\u4e2a\u5f3a\u5927\u7684HCC\u4e9a\u578b<\/span><\/p>\n

\u4eceAgingAtlas\u3001HAGR\u3001KEGG\u548cReactome\u6570\u636e\u5e93\u4e2d\u5168\u9762\u68c0\u7d22\u4e86\u8870\u8001\u57fa\u56e0\u96c6\uff0c\u8fd9\u4e9b\u6570\u636e\u5e93\u8bb0\u5f55\u4e86\u8c03\u8282\u7ec6\u80de\u8870\u8001\u3001\u957f\u5bff\u6216\u4e0e\u8870\u8001\u76f8\u5173\u7684\u57fa\u56e0\u3002\u5171\u4fdd\u7559\u4e86\u51fa\u73b0\u4e00\u6b21\u4ee5\u4e0a\u7684 368 \u4e2a\u57fa\u56e0\u4f5c\u4e3a\u6700\u7ec8\u7684 SRG\uff08\u56fe 1A \uff09\u3002\u901a\u8fc7\u65e0\u76d1\u7763\u805a\u7c7b\u65b9\u6cd5\uff0c\u5229\u7528SRGs\u53ef\u5c06LIHC\u60a3\u8005\u5206\u4e3a\u4e09\u4e2a\u4e9a\u578b\uff0c\u5373ClusterA\u3001ClusterB\u548cClusterC\uff08\u56fe1B\uff09\u3002\u8fdb\u884c\u4e86ssGSEA\uff0c\u4ee5\u6d4b\u91cf\u4e09\u4e2a\u4e9a\u578b\u4e2d\u8870\u8001\u901a\u8def\u7684\u6fc0\u6d3b\u60c5\u51b5\uff0c\u7ed3\u679c\u8868\u660eClusterC\u7684\u5bcc\u96c6\u7a0b\u5ea6\u660e\u663e\u66f4\u9ad8\uff08\u56fe 1C\uff09\u3002GLB1\u3001TP53\u3001CDKN1A (p21)\u3001CDKN2A (p16)\u7b49\u5e7f\u4e3a\u63a5\u53d7\u7684\u8870\u8001\u6807\u5fd7\u7269\u7684\u8868\u8fbe\u91cf\u4e5f\u4ece ClusterA \u589e\u52a0\u5230 ClusterC\uff08\u56fe 1D\uff09\u3002\u751f\u5b58\u5206\u6790\u8868\u660e\uff0cClusterC \u60a3\u8005\u7684\u9884\u540e\u6700\u5dee\uff0c\u8fd9\u8868\u660e ClusterC \u53ef\u80fd\u4ee3\u8868\u4e86\u4e00\u4e2a\u9ad8\u98ce\u9669\u4e9a\u7fa4\uff08\u56fe 1E-H\uff09\u3002<\/span>\u603b\u4e4b\uff0c\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\uff0cSRGs \u5bf9 HCC \u60a3\u8005\u5177\u6709\u5f88\u5f3a\u7684\u9884\u6d4b\u80fd\u529b\u3002<\/span>    <\/span><\/o:page><\/p>\n

\"1\u533a10\u5206+\u9884\u540e\u6587\u7ae0\u6709\u4f55\u4e7e\u5764\uff1f\u534e\u897f\u533b\u9662\u56e2\u961f\uff1a\u8870\u8001\u7279\u5f81\u57fa\u56e0+\u591a\u7ec4\u5b66\u5206\u6790\uff0c\u7ecf\u5178\u601d\u8def\u89e3\u9501\u9690\u85cf\u65b0\u73a9\u6cd5\uff01\uff01\"<\/span><\/p>\n

\u56fe1 \u6839\u636e SRGs \u786e\u5b9a\u4e09\u4e2a\u7a33\u5065\u7684 HCC \u4e9a\u578b\u53ca\u5176\u7279\u5f81<\/span><\/p>\n

2. \u8870\u8001\u4e9a\u578b\u7684\u53d8\u5f02\u7279\u5f81<\/span><\/p>\n

\u8003\u8651\u5230\u57fa\u56e0\u7ec4\u53d8\u5f02\uff0c\u5bf9\u5355\u6838\u82f7\u9178\u591a\u6001\u6027\uff08SNP\uff09\u3001\u5c0fINDEL\u548c\u62f7\u8d1d\u6570\u53d8\u5f02\uff08CNV\uff09\u8fdb\u884c\u4e86\u5206\u6790\u3002\u5217\u51fa\u4e86\u6bcf\u4e2a\u4e9a\u578b\u7684\u524d10\u4e2a\u9ad8\u7a81\u53d8\u57fa\u56e0\uff0c\u5176\u4e2dTP53\u3001RB1\u548cCSMD1\u5728ClusterC\u4e2d\u7684\u7a81\u53d8\u9891\u7387\u8f83\u9ad8\uff0c\u800cCTNNB1\u7684\u7a81\u53d8\u9891\u7387\u8f83\u4f4e\uff08\u56fe2A-B\uff09\u3002\u5728\u5171\u73b0\u548c\u4e92\u65a5\u4e8b\u4ef6\u65b9\u9762\uff0cClusterA\u4e2dTTN\u4e0eAHNAK2\u3001MUC16\u4e0eRYR2\u3001RYR3\u4e0eUSH2A\u4e4b\u95f4\u5b58\u5728\u663e\u8457\u7684\u5171\u7a81\u53d8\u3002\u4e0e\u5176\u4ed6\u4e24\u4e2a\u4e9a\u578b\u76f8\u6bd4\uff0cClusterB\u4e2d\u7684\u5171\u73b0\u4e8b\u4ef6\u76f8\u5bf9\u8f83\u591a\uff0cCTNNB1\u4e0eAPOB\u3001OBSCN\u4e0eFLG\u3001DNAH7\u4e0ePCLO\u4e4b\u95f4\u7684\u5171\u73b0\u4e8b\u4ef6\u663e\u8457\u3002\u5728ClusterC\u4e2d\uff0cTP53\u4e0ePCLO\u3001RYR1\u4e0eADGRV1\u3001ABCA13\u4e0ePCLO\u4e4b\u95f4\u5b58\u5728\u663e\u8457\u7684\u5171\u7a81\u53d8\uff08\u56fe2C\uff09\u3002\u5229\u7528 GISTIC2 \u68c0\u6d4b\u4e86\u8870\u8001\u4e9a\u578b\u4e2d\u7684\u5f02\u5e38 CNV \u533a\u57df\u3002\u6240\u6709\u4e9a\u578b\u90fd\u663e\u793a\u51fa 8q24 \u7ec6\u80de\u5e26\u7684\u6269\u589e\u4ee5\u53ca 1p36 \u548c 8p23 \u7ec6\u80de\u5e26\u7684\u7f3a\u5931\u3002\u800c\u5728ClusterB\u4e2d\uff0c6\u53f7\u67d3\u8272\u4f53\u6709\u7279\u6b8a\u7684\u6269\u589e\u3002\u800c\u5728 ClusterC \u4e2d\uff0c3 \u53f7\u67d3\u8272\u4f53\u51fa\u73b0\u4e86\u72ec\u7279\u7684\u7f3a\u5931\uff08\u56fe 2D\uff09\u3002<\/span>\u4e0a\u8ff0\u7ed3\u679c\u8868\u660e\u4e09\u4e2a\u4e9a\u578b\u5b58\u5728\u4e0d\u540c\u7684\u4f53\u7ec6\u80de\u7a81\u53d8\u3002<\/span>    <\/span><\/o:page><\/p>\n

\"1\u533a10\u5206+\u9884\u540e\u6587\u7ae0\u6709\u4f55\u4e7e\u5764\uff1f\u534e\u897f\u533b\u9662\u56e2\u961f\uff1a\u8870\u8001\u7279\u5f81\u57fa\u56e0+\u591a\u7ec4\u5b66\u5206\u6790\uff0c\u7ecf\u5178\u601d\u8def\u89e3\u9501\u9690\u85cf\u65b0\u73a9\u6cd5\uff01\uff01\"<\/span><\/p>\n

\u56fe2 \u4e09\u4e2a\u8870\u8001\u4e9a\u578b\u7684\u7a81\u53d8\u60c5\u51b5<\/span><\/p>\n

3. \u8870\u8001\u4e9a\u578b\u7684\u514d\u75ab\u7279\u5f81\u5206\u6790<\/span><\/p>\n

\u80bf\u7624\u5fae\u73af\u5883\uff08TME\uff09\u662f\u6307\u6076\u6027\u7ec6\u80de\u4e0e\u514d\u75ab\u7ec6\u80de\u548c\u57fa\u8d28\u7ec6\u80de\u76f8\u4e92\u4f5c\u7528\uff0c\u5171\u540c\u4fc3\u8fdb\u6216\u6291\u5236\u80bf\u7624\u8fdb\u5c55\u7684\u573a\u6240\u3002\u5305\u62ec CD274 (PD-L1)\u3001PDCD1 (PD1)\u3001CTLA4 \u7b49\u5728\u5185\u7684\u591a\u79cd\u514d\u75ab\u53cd\u5e94\u5206\u5b50\u5728 ClusterC \u4e2d\u7684\u8868\u8fbe\u91cf\u663e\u8457\u5347\u9ad8\uff08\u56fe 3A\uff09\u3002\u4f5c\u8005\u8fdb\u4e00\u6b65\u5229\u7528ESTIMATE\u7b97\u6cd5\u5206\u6790\u4e86\u4e09\u4e2a\u8870\u8001\u96c6\u4e9a\u578b\u7684\u6574\u4f53TME\u72b6\u6001\u3002\u6240\u6709\u7684\u514d\u75ab\u5f97\u5206\u3001\u57fa\u8d28\u5f97\u5206\u548cESTIMATE\u5f97\u5206\u5728ClusterC\u4e2d\u90fd\u662f\u6700\u9ad8\u7684\uff0c\u8fd9\u8868\u660e\u514d\u75ab\u7ec6\u80de\u548c\u57fa\u8d28\u7ec6\u80de\u7684\u6210\u5206\u5728ClusterC\u4e2d\u66f4\u4e3a\u4e30\u5bcc\uff08\u56fe3B\uff09\u3002ssGSEA \u5bf9\u4e09\u4e2a\u8870\u8001\u4e9a\u578b\u7684\u514d\u75ab\u6d78\u6da6\u8fdb\u884c\u4e86\u5206\u6790\u3002T \u7ec6\u80de\u7684\u6bd4\u4f8b\u5728 ClusterC \u4e2d\u66f4\u9ad8\uff0c\u800c\u4e2d\u6027\u7c92\u7ec6\u80de\u7684\u6bd4\u4f8b\u5728 ClusterA \u4e2d\u66f4\u5145\u8db3\uff08\u56fe 3C\uff09\u3002\u4f5c\u8005\u5206\u6790\u4e86\u5df2\u53d1\u8868\u7684\u514d\u75ab\u80bf\u7624\u751f\u7269\u5b66\u7814\u7a76\uff08IOBR\uff09\u4e2d\u6d89\u53ca\u514d\u75ab\u8017\u7aed\u3001\u514d\u75ab\u6291\u5236\u548c\u514d\u75ab\u6392\u65a5\u7684\u7279\u5f81\uff0c\u4ee5\u63a2\u7d22\u514d\u75ab\u6cbb\u7597\u6f5c\u529b\u7684\u591a\u6837\u6027\u3002\u8868\u660e\u514d\u75ab\u9003\u907f\u6216\u6291\u5236\u7684\u7279\u5f81\uff0c\u5982<\/span>T\u7ec6\u80de\u8c03\u8282\u3001\u9ad3\u6e90\u6291\u5236\u7ec6\u80de\uff08MDSC\uff09\u548c\u764c\u76f8\u5173\u6210\u7ea4\u7ef4\u7ec6\u80de<\/span>\uff08CAFs\uff09\uff0c\u4ee5\u53ca<\/span>EMT\u3001WNT\u548cTGFb\u4fe1\u53f7\u901a\u8def<\/span>\u7684\u6fc0\u6d3b\uff0c\u5728<\/span>ClusterC\u4e2d\u6700\u4e3a\u5bcc\u96c6<\/span>\uff08\u56fe3D-F\uff09\u3002<\/span>    <\/span><\/o:page><\/p>\n

\"1\u533a10\u5206+\u9884\u540e\u6587\u7ae0\u6709\u4f55\u4e7e\u5764\uff1f\u534e\u897f\u533b\u9662\u56e2\u961f\uff1a\u8870\u8001\u7279\u5f81\u57fa\u56e0+\u591a\u7ec4\u5b66\u5206\u6790\uff0c\u7ecf\u5178\u601d\u8def\u89e3\u9501\u9690\u85cf\u65b0\u73a9\u6cd5\uff01\uff01\"<\/span>    <\/span><\/o:page><\/p>\n

\u56fe3 \u4e09\u4e2a\u8870\u8001\u4e9a\u578b\u7684\u80bf\u7624\u514d\u75ab\u5fae\u73af\u5883<\/span><\/p>\n

4. \u805a\u7c7b\u548c\u63d0\u53d6\u8870\u8001\u6807\u8bb0\u4ee5\u91cf\u5316\u8870\u8001<\/span><\/p>\n

\u6839\u636eSRGs\u5728\u4e09\u4e2a\u8870\u8001\u4e9a\u578b\u4e2d\u7684\u8868\u8fbe\u60c5\u51b5\u5bf9\u5176\u8fdb\u884c\u5206\u7ec4\u3002\u5728 LIHC \u548c LIRI \u6570\u636e\u96c6\u4e2d\u53d1\u73b0\u4e86\u56db\u4e2a\u57fa\u56e0\u7fa4\u3002\u5728\u8fd9\u4e9b\u57fa\u56e0\u7c07\u4e2d\uff0cLIHC \u4e2d\u7684\u7b2c 4 \u7c07\uff08n = 163\uff09\u548c LIRI \u4e2d\u7684\u7b2c 2 \u7c07\uff08n = 146\uff09\u56e0\u5176\u6709\u89c4\u5f8b\u7684\u8868\u8fbe\u53d8\u5316\u800c\u663e\u793a\u51fa\u533a\u5206\u4e09\u4e2a\u8870\u8001\u7c07\u7684\u5de8\u5927\u6f5c\u529b\uff08\u56fe 4A-B\uff09\u3002\u901a\u8fc7\u4ea4\u53c9\u4e24\u4e2a\u57fa\u56e0\u7c07\u4e4b\u95f4\u7684\u91cd\u53e0\u57fa\u56e0\uff0c\u5171\u63d0\u53d6\u4e86 100 \u4e2a\u57fa\u56e0\uff0c\u5e76\u5c06\u5176\u5b9a\u4e49\u4e3a Sene.marker\uff08\u56fe 4C\uff09\u3002\u5728\u51e0\u4e2a\u6570\u636e\u96c6\u4e2dSene.marker\u7684\u5e73\u5747\u8868\u8fbe\u91cf\u4e0e\u8870\u8001\u6807\u8bb0\u7684\u8868\u8fbe\u91cf\u5448\u6b63\u76f8\u5173\uff08\u56fe 4D\uff09\u3002\u4f7f\u7528 GSEA \u6bd4\u8f83\u8fd9\u4e9b\u961f\u5217\u4e2d Sene.High \u7ec4\u548c Sene.Low \u7ec4\u7684\u5168\u5c40\u8f6c\u5f55\u7ec4\u8868\u8fbe\uff0c\u7ed3\u679c\u663e\u793a\u524d\u8005\u51e0\u4e4e\u5bcc\u96c6\u4e86\u8870\u8001\u901a\u8def\u7684\u6d3b\u6027\uff08\u56fe 4E\uff09\u3002<\/span>\u603b\u4e4b\uff0c\u8fd9\u4e9b\u7ed3\u679c\u4e3a\u5256\u6790 HCC \u4e2d\u7684\u8870\u8001\u63d0\u4f9b\u4e86\u8bc1\u636e\u3002<\/span>    <\/span><\/o:page><\/p>\n

\"1\u533a10\u5206+\u9884\u540e\u6587\u7ae0\u6709\u4f55\u4e7e\u5764\uff1f\u534e\u897f\u533b\u9662\u56e2\u961f\uff1a\u8870\u8001\u7279\u5f81\u57fa\u56e0+\u591a\u7ec4\u5b66\u5206\u6790\uff0c\u7ecf\u5178\u601d\u8def\u89e3\u9501\u9690\u85cf\u65b0\u73a9\u6cd5\uff01\uff01\"<\/span><\/p>\n

\u56fe4 \u4ece\u4e09\u4e2a\u4e9a\u578b\u4e2d\u8bc6\u522b\u8870\u8001\u6807\u8bb0<\/span><\/p>\n

5. \u8870\u8001\u4e0e\u5e72\u6027\u4e4b\u95f4\u7684\u8054\u7cfb<\/span><\/p>\n

\u5229\u7528ssGSEA\u6765\u6d4b\u91cf\u6bcf\u4f4d\u60a3\u8005\u5e72\u6027\u76f8\u5173\u7279\u5f81\u7684\u5bcc\u96c6\u7a0b\u5ea6\u3002\u7ed3\u679c\u663e\u793a\uff0cClusterC\u6216Sene.High\u7ec4\u7684\u5e72\u7ec6\u80de\u76f8\u5173\u7279\u5f81\u6d3b\u6027\u6700\u9ad8\uff08\u56fe5A-B\uff09\u3002\u8fd8\u89c2\u5bdf\u5230\u8870\u8001\u548c\u5e72\u6027\u6807\u5fd7\u7269\u5982EPCAM\u3001KRT19\u3001ANXA3\u3001KLF4\u3001CD44\u548cPROM1\u7684\u8868\u8fbe\u4e4b\u95f4\u5b58\u5728\u6b63\u76f8\u5173\uff08\u56fe5C\uff09\u3002\u6cbb\u7597\u8bf1\u5bfc\u8870\u8001\uff08TIS\uff09\u901a\u5e38\u51fa\u73b0\u5728\u63a5\u53d7\u6297\u764c\u836f\u7269\u6cbb\u7597\u7684\u80bf\u7624\u7ec6\u80de\u4e2d\uff0c\u8fd9\u53cd\u8fc7\u6765\u53c8\u4f1a\u5ef6\u957f\u5b83\u4eec\u7684\u751f\u5b58\u671f\u5e76\u6700\u7ec8\u5bfc\u81f4\u590d\u53d1\u3002\u4e4b\u524d\u7684\u7814\u7a76\u62a5\u9053\u4e86\u591a\u79cd\u7c7b\u578b\u7684\u80bf\u7624\u7ec6\u80de\u5728\u591a\u67d4\u6bd4\u661f\u5e72\u9884\u540e\u4ee5 p53\/p21 \u4f9d\u8d56\u6027\u7684\u65b9\u5f0f\u53d1\u751f TIS\u3002\u5728\u6b64\uff0cHuh7 \u7ec6\u80de\u7ecf\u591a\u67d4\u6bd4\u661f\u5904\u7406 4 \u5929\u540e\uff0c\u5728\u4e0d\u542b\u591a\u67d4\u6bd4\u661f\u7684\u57f9\u517b\u57fa\u4e2d\u57f9\u517b\uff0c\u6bcf 2 \u5929\u66f4\u6362\u4e00\u6b21\u57f9\u517b\u57fa\uff0c\u76f4\u81f3 15 \u5929\uff0c\u751f\u6210\u4f53\u5916 HCC \u8870\u8001\u6a21\u578b\uff08\u56fe 5D\uff09\u3002\u5728\u591a\u67d4\u6bd4\u661f\u5904\u7406\u540e\uff0cHuh7\u7ec6\u80de\u8868\u73b0\u51fa\u8870\u8001\uff0cSA-\u03b2-gal\u67d3\u8272\u663e\u793a\u4e86\u8fd9\u4e00\u70b9\uff08\u56fe 5E\uff09\u3002\u4e0e\u5176\u4ed6\u4e9a\u578b\u76f8\u6bd4\uff0c\u4ee3\u8868\u8870\u8001\u72b6\u6001\u7684ClusterC\u4e2d\u5e72\u6027\u6807\u8bb0\u7269\u7684\u8868\u8fbe\u66f4\u9ad8\uff08\u56fe5F\uff09\u3002\u5728TIS\u6a21\u578b\u4e2d\u4e5f\u89c2\u5bdf\u5230\u4e86\u4e00\u81f4\u7684\u7ed3\u679c\uff08\u56fe5G\uff09\u3002<\/span>\u7efc\u4e0a\u6240\u8ff0\uff0c\u8fd9\u4e9b\u53d1\u73b0\u8bc1\u5b9e\u836f\u7269\u6cbb\u7597\u540e\u5e72\u6027\u91cd\u7f16\u7a0b\uff0c\u5e76\u4e0e\u8870\u8001\u76f8\u5173\u3002<\/span>    <\/span><\/o:page><\/p>\n

\"1\u533a10\u5206+\u9884\u540e\u6587\u7ae0\u6709\u4f55\u4e7e\u5764\uff1f\u534e\u897f\u533b\u9662\u56e2\u961f\uff1a\u8870\u8001\u7279\u5f81\u57fa\u56e0+\u591a\u7ec4\u5b66\u5206\u6790\uff0c\u7ecf\u5178\u601d\u8def\u89e3\u9501\u9690\u85cf\u65b0\u73a9\u6cd5\uff01\uff01\"<\/span>    <\/span><\/o:page><\/p>\n

\u56fe5 HCC \u8870\u8001\u8fc7\u7a0b\u4e2d\u7684\u5e72\u6027\u91cd\u7f16\u7a0b<\/span><\/p>\n

6. \u9884\u6d4bSene.marker\u7684\u6f5c\u5728\u8870\u8001\u7f13\u89e3\u548c\u514d\u75ab\u7597\u6cd5\u7597\u6548<\/span><\/p>\n

\u4e3a\u4e86\u8fdb\u4e00\u6b65\u4e86\u89e3\u8870\u8001\u7fa4\u7684\u4e34\u5e8a\u5e94\u7528\uff0c\u8bc4\u4f30\u4e86LIHC\u60a3\u8005\u7684\u836f\u7269\u654f\u611f\u6027\u4e0eSene.marker\u5e73\u5747\u8868\u8fbe\u91cf\u4e4b\u95f4\u7684\u5173\u8054\u3002\u5171\u53d1\u73b082\u79cd\u836f\u7269\u5448\u663e\u8457\u8d1f\u76f8\u5173\uff0c\u8fd9\u4e9b\u836f\u7269\u88ab\u8ba4\u4e3a\u662f\u654f\u611f\u836f\u7269\uff0c\u800c\u53ea\u67093\u79cd\u836f\u7269\u5448\u6b63\u76f8\u5173\uff08\u56fe6A\uff09\u3002\u5176\u4e2d\uff0c\u89c2\u5bdf\u5230\u8f83\u591a\u9776\u5411\u6709\u4e1d\u5206\u88c2\u3001\u51cb\u4ea1\u8c03\u63a7\u548c PI3K\/MTOR \u901a\u8def\u7684\u836f\u7269\uff0c\u4e3a\u5f00\u53d1\u7ba1\u7406\u80bf\u7624\u8870\u8001\u7684\u836f\u7269\u63d0\u4f9b\u4e86\u65b0\u7684\u601d\u8def\uff08\u56fe 6B\uff09\u3002\u89c2\u5bdf\u5230\u5316\u7597\u836f\u7269SB505124\u3001AZD2014\u548cSelumetinib\u5bf9Sene.marker\u8868\u73b0\u51fa\u8010\u836f\u6027\uff0c\u4f46\u53ef\u80fd\u662f\u5c5e\u4e8eClusterA\u7684\u60a3\u8005\u7684\u5408\u9002\u6cbb\u7597\u836f\u7269\uff08\u56fe6C-E\uff09\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u836f\u7269Tozasertib\u3001Osimertinib\u548cUMI-77\u5bf9Sene.marker\u6700\u654f\u611f\uff0c\u8fd9\u8868\u660e\u5b83\u4eec\u6709\u53ef\u80fd\u6210\u4e3aClusterC\u7684\u89e3\u6bd2\u5242\uff08\u56fe6F-H\uff09\u3002\u8fd9\u4e9b\u7ed3\u679c<\/span>\u4e3a\u4e0d\u540c\u7684\u8870\u8001\u7ec4\u63d0\u4f9b\u4e86\u91cf\u8eab\u5b9a\u5236\u7684\u6cbb\u7597\u7b56\u7565<\/span>\u3002\u8fd8\u7814\u7a76\u4e86 Sene.marker \u9884\u6d4b\u514d\u75ab\u7597\u6cd5\u60a3\u8005\u53cd\u5e94\u7684\u80fd\u529b\u3002\u5728\u4e24\u4e2a\u72ec\u7acb\u961f\u5217\u4e2d\uff0cSene.marker\u8f83\u9ad8\u7684\u60a3\u8005\u603b\u751f\u5b58\u671f\u5ef6\u957f\uff08\u56fe 6I\uff0cL\uff09\u3002\u8fd9\u53ef\u80fd\u662f\u7531\u4e8e\u80bf\u7624\u8870\u8001\u8fc7\u7a0b\u4e2d\u8868\u8fbe\u7684\u514d\u75ab\u68c0\u67e5\u70b9\u5206\u5b50\u6c34\u5e73\u8f83\u9ad8\uff0c\u4ee5\u53ca\u514d\u75ab\u6291\u5236\u7ec6\u80de\u7684\u5bcc\u96c6\uff08\u56fe 3\uff09\u3002\u8fd9\u4e9b\u7279\u5f81\u4e0e\u514d\u75ab\u6cbb\u7597\u53cd\u5e94\u7684\u589e\u5f3a\u76f8\u5173\uff0c\u5e76\u786e\u4fdd\u60a3\u8005\u4ece\u514d\u75ab\u7cfb\u7edf\u7684\u91cd\u65b0\u6fc0\u6d3b\u4e2d\u83b7\u76ca\u3002\u6b64\u5916\uff0c\u5728 IMvigor210 \u4e2d\uff0cSene.High \u7ec4\u60a3\u8005\u7684\u80bf\u7624\u7a81\u53d8\u8d1f\u8377\u548c\u65b0\u6297\u539f\u8d1f\u8377\u660e\u663e\u8f83\u9ad8\uff08\u56fe 6J-K\uff09\uff0c\u800c\u65b0\u6297\u539f\u7f16\u7801\u7684\u5f02\u5e38\u86cb\u767d\u53ef\u88ab\u514d\u75ab\u7ec6\u80de\u8bc6\u522b\u5e76\u6d88\u706d\u3002\u56e0\u6b64\uff0c<\/span>\u8870\u8001\u8bc4\u5206\u8f83\u9ad8\u7684\u60a3\u8005\u53ef\u4ee5\u5229\u7528\u514d\u75ab\u7597\u6cd5\u3002<\/span>    <\/span><\/o:page><\/p>\n

\"1\u533a10\u5206+\u9884\u540e\u6587\u7ae0\u6709\u4f55\u4e7e\u5764\uff1f\u534e\u897f\u533b\u9662\u56e2\u961f\uff1a\u8870\u8001\u7279\u5f81\u57fa\u56e0+\u591a\u7ec4\u5b66\u5206\u6790\uff0c\u7ecf\u5178\u601d\u8def\u89e3\u9501\u9690\u85cf\u65b0\u73a9\u6cd5\uff01\uff01\"<\/span>    <\/span><\/o:page><\/p>\n

\u56fe6 Sene.marker \u4e0e\u836f\u7269\u654f\u611f\u6027\u548c\u514d\u75ab\u7597\u6cd5\u7597\u6548\u4e4b\u95f4\u7684\u5173\u7cfb<\/span><\/p>\n

7. SRGA \u6709\u52a9\u4e8e\u9274\u5b9a\u65b0\u7684\u8870\u8001\u76f8\u5173\u57fa\u56e0<\/span><\/p>\n

\u4e3a\u4e86\u9274\u5b9a\u4e0e\u8870\u8001\u76f8\u5173\u7279\u5f81\u76f8\u5173\u7684\u5019\u9009\u57fa\u56e0\uff0c\u4f5c\u8005\u5f00\u53d1\u4e86\u4e00\u4e2a\u540d\u4e3a SRGA \u7684 R \u8f6f\u4ef6\u5305\uff08\u56fe 7A\uff09\u3002SRGA \u6574\u5408\u5e76\u4fee\u6539\u4e86 ImmLnc \u548c GSEA \u7684\u6846\u67b6\uff0c\u5229\u7528\u5927\u91cf\u8f6c\u5f55\u7ec4\u6570\u636e\u548c\u5148\u9a8c\u751f\u7269\u5b66\u77e5\u8bc6\u6765\u8bc6\u522b\u4e0e\u514d\u75ab\u3001\u8870\u8001\u7b49\u611f\u5174\u8da3\u7684\u8868\u578b\u76f8\u5173\u7684\u65b0\u6807\u8bb0\uff0c\u6216\u53ef\u6269\u5c55\u5230\u764c\u75c7\u8f6c\u79fb\u548c\u8010\u836f\u6027\u3002\u540c\u65f6\uff0c\u901a\u8fc7\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u4e3a\u6bcf\u4e2a\u8870\u8001\u7c07\u63d0\u53d6\u4e86\u6807\u8bb0\u57fa\u56e0\uff0c\u7ed3\u679c\u662fClusterA\u670922\u4e2a\u57fa\u56e0\uff0cClusterB\u6709132\u4e2a\u57fa\u56e0\uff0cClusterC\u67092090\u4e2a\u57fa\u56e0\u3002\u901a\u8fc7\u4f7f\u7528\u6765\u81ea AgingAtlas \u7684 12 \u4e2a\u901a\u8def\u4ee5\u53ca\u9884\u5148\u5b9a\u4e49\u7684 Sene.marker \u4f5c\u4e3a\u7279\u5f81\uff0c\u5e76\u5c06\u603b\u5171 2244 \u4e2a\u4e9a\u578b\u6807\u8bb0\u4f5c\u4e3a\u8f93\u5165\uff0c\u5e94\u7528 SRGA\uff0c\u83b7\u5f97\u4e86\u4e0e\u6bcf\u4e2a\u7279\u5f81\u76f8\u5173\u7684\u57fa\u56e0\u3002\u8fd9\u4e9b\u7279\u5f81\u6d89\u53ca 17 \u81f3 2009 \u4e2a\u57fa\u56e0\uff08\u56fe 7B\uff09\u3002\u6b64\u5916\uff0c\u8fd8\u6784\u5efa\u4e86\u524d 5 \u4e2a\u7279\u5f81\u57fa\u56e0\u5bf9\u7f51\u7edc\uff0c\u5176\u4e2d UBD\u3001SPINK1\u3001CENPU \u548c HLA-DRA \u5206\u522b\u4e0e\u4e24\u4e2a\u4e0d\u540c\u7684\u7279\u5f81\u663e\u8457\u76f8\u5173\uff08\u56fe 7C\uff09\u3002\u53e6\u5916\uff0c\u4f5c\u8005\u8fd8\u6839\u636e\u57fa\u56e0\u4e0e\u8870\u8001\u7279\u5f81\u7684\u8ba1\u7b97\u5f97\u5206\u5bf9\u57fa\u56e0\u8fdb\u884c\u4e86\u6392\u5e8f\uff08\u56fe 7D\uff09\u3002\u4e3a\u4e86\u627e\u51fa\u4e0e\u9884\u540e\u76f8\u5173\u7684\u57fa\u56e0\uff0c\u56e2\u961f\u5c06\u524d 20 \u4e2a\u57fa\u56e0\u7eb3\u5165\u4e86 LIHC \u548c LIRI \u6570\u636e\u96c6\u7684 Cox \u548c Kaplan-Meier \u6a21\u578b\u3002\u6700\u540e\uff0c<\/span>MAFG\u3001TUBA1C\u3001CSTF2\u3001PLIN3\u3001FAM83D \u548c MMP12<\/span> \u8fd9\u516d\u4e2a\u57fa\u56e0\u88ab\u9009\u4e3a\u4e0e\u60a3\u8005\u751f\u5b58\u76f8\u5173\u7684\u65b0\u8870\u8001\u76f8\u5173\u57fa\u56e0\u3002<\/span>    <\/span><\/o:page><\/p>\n

\"1\u533a10\u5206+\u9884\u540e\u6587\u7ae0\u6709\u4f55\u4e7e\u5764\uff1f\u534e\u897f\u533b\u9662\u56e2\u961f\uff1a\u8870\u8001\u7279\u5f81\u57fa\u56e0+\u591a\u7ec4\u5b66\u5206\u6790\uff0c\u7ecf\u5178\u601d\u8def\u89e3\u9501\u9690\u85cf\u65b0\u73a9\u6cd5\uff01\uff01\"<\/span><\/p>\n

\u56fe7 \u9274\u5b9a\u65b0\u7684\u8870\u8001\u6807\u8bb0<\/span>    <\/span><\/o:page><\/p>\n

8. \u9a8c\u8bc1\u65b0\u7684\u8870\u8001\u6807\u8bb0<\/span><\/p>\n

\u4f7f\u7528\u591a\u67d4\u6bd4\u661f\u5904\u7406\u4e86Huh7\u7ec6\u80de\uff0c\u7528\u4e0d\u540c\u6d53\u5ea6\u7684\u591a\u67d4\u6bd4\u661f\u5904\u7406Huh7\u7ec6\u80de4\u5929\uff0c\u6216\u752850 nM\u6d53\u5ea6\u7684\u591a\u67d4\u6bd4\u661f\u5904\u7406\u4e0d\u540c\u65f6\u95f4\uff0cHuh7\u7ec6\u80de\u90fd\u8868\u73b0\u51fa\u660e\u663e\u7684\u8870\u8001\uff0c\u5305\u62ec\u5f62\u6001\u53d8\u5927\u53d8\u6241\uff08\u56fe8A\uff09\uff0cSA-\u03b2-Gal\u67d3\u8272\u589e\u5f3a\uff08\u56fe8B\uff09\uff0cp21\u548cp53\u86cb\u767d\u8868\u8fbe\u589e\u52a0\uff08\u56fe8C\uff09\u3002\u6b64\u5916\uff0c\u5f53Huh7\u53d1\u751f\u8870\u8001\u65f6\uff0cMAFG\u548cPLIN3\u7684\u86cb\u767d\u8868\u8fbe\u4e5f\u5347\u9ad8\uff08\u56fe8C\uff09\u3002\u540c\u6837\uff0c\u5728\u5242\u91cf\u548c\u65f6\u95f4\u4f9d\u8d56\u6027\u6a21\u578b\u4e2d\uff0c\u65b0\u53d1\u73b0\u7684\u8870\u8001\u6807\u8bb0\u7269\u7684 mRNA \u8868\u8fbe\u4e5f\u589e\u52a0\u4e86\uff08\u56fe 8D-E\uff09\u3002\u8fd9\u4e9b\u6570\u636e\u8868\u660e\uff0c<\/span>SRGA \u5728\u9274\u5b9a\u67d0\u4e9b\u8868\u578b\u7684\u65b0\u6807\u8bb0\u7269\u65b9\u9762\u662f\u5207\u5b9e\u53ef\u884c\u548c\u53ef\u4fe1\u7684\u3002<\/span>    <\/span><\/o:page><\/p>\n

\"1\u533a10\u5206+\u9884\u540e\u6587\u7ae0\u6709\u4f55\u4e7e\u5764\uff1f\u534e\u897f\u533b\u9662\u56e2\u961f\uff1a\u8870\u8001\u7279\u5f81\u57fa\u56e0+\u591a\u7ec4\u5b66\u5206\u6790\uff0c\u7ecf\u5178\u601d\u8def\u89e3\u9501\u9690\u85cf\u65b0\u73a9\u6cd5\uff01\uff01\"<\/span><\/p>\n

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9.<\/span> MAFG\u8c03\u63a7HCC\u7684\u8870\u8001\u76f8\u5173\u8868\u578b\u548cMAFG \u5728\u8870\u8001\u7ec6\u80de\u4e2d\u7684\u514d\u75ab\u6291\u5236\u4f5c\u7528<\/span><\/span><\/p>\n

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