{"id":43961,"date":"2024-06-02T01:20:30","date_gmt":"2024-06-01T17:20:30","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=43961"},"modified":"2024-06-02T17:29:21","modified_gmt":"2024-06-02T09:29:21","slug":"a26f907025","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=43961","title":{"rendered":"\u5929\u5450\uff01\u516c\u5171\u6570\u636e\u5e93\u8fd8\u80fd\u8fd9\u4e48\u7528\uff1f\u6210\u90fd\u533b\u5b66\u9662\u66fe\u660e\u5f3a\u56e2\u961f1\u533a\u65b0\u4f5c\uff01GEO\u6570\u636e\u5e93+CellAge\u6570\u636e\u5e93+\u7efc\u5408\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u5feb\u6765\u5b66\u5440\uff01"},"content":{"rendered":"
\n

\"\u5929\u5450\uff01\u516c\u5171\u6570\u636e\u5e93\u8fd8\u80fd\u8fd9\u4e48\u7528\uff1f\u6210\u90fd\u533b\u5b66\u9662\u66fe\u660e\u5f3a\u56e2\u961f1\u533a\u65b0\u4f5c\uff01GEO\u6570\u636e\u5e93+CellAge\u6570\u636e\u5e93+\u7efc\u5408\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u5feb\u6765\u5b66\u5440\uff01\"<\/p>\n

\u6628\u5929\u6709\u5c0f\u4f19\u4f34\u79c1\u4fe1\u5c0f\u4e91\uff0c\u89c9\u5f97\u516c\u5171\u6570\u636e\u5e93\u4e2d\u6570\u636e\u96c6\u5f80\u5f80\u7ecf\u8fc7\u5927\u91cf\u5206\u6790\uff0c\u96be\u4ee5\u521b\u65b0\u3002\u6709\u4e00\u5b9a\u9053\u7406\uff0c\u4f46\u53ea\u8981\u7528\u5bf9\u601d\u8def\uff0c\u7efc\u5408\u5229\u7528\u516c\u5171\u6570\u636e\u5e93\uff0c\u4e00\u6837\u53ef\u4ee5\u8f7b\u677e\u53d1\u6587\u7ae0\uff01\u4eca\u5929\u5c0f\u4e91\u5c31\u7ed9\u5927\u5bb6\u5206\u4eab\u4e00\u7bc7\u3002\u672c\u6587\u7efc\u5408\u5229\u7528\u4e86\u4e24\u4e2a\u6570\u636e\u5e93\uff0c\u91c7\u7528\u591a\u79cd\u751f\u7269\u4fe1\u606f\u5b66\u65b9\u6cd5\uff0c\u5206\u6790\u4e86\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\u548c\u7ec6\u80de\u8870\u8001\u76f8\u5173\u7684\u57fa\u56e0\u548c\u901a\u8def\u3002\u8fd9\u7bc7\u8bba\u6587\u662f\u5982\u4f55\u505a\u7684\u5462\uff1f\u5c0f\u4e91<\/span>\u5e26\u5c0f\u4f19\u4f34\u4eec\u4e00\u8d77\u63ed\u79d8\u4e00\u4e0b\u5427~<\/span><\/span><\/span><\/p>\n

1\uff0c<\/span>\u516c\u5171\u6570\u636e\u5e93\u7684\u7efc\u5408\u5229\u7528<\/span><\/span>\uff1a<\/span>\u6587\u7ae0\u901a\u8fc7\u6574\u5408\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u96c6\u548c\u7ec6\u80de\u8870\u8001\u6570\u636e\u5e93\uff0c\u7814\u7a76\u8bc6\u522b\u4e86\u4e00\u7ec4\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\uff08DEGs\uff09\uff0c\u5e76\u8fdb\u4e00\u6b65\u5bf9\u8fd9\u4e9b\u57fa\u56e0\u8fdb\u884c\u529f\u80fd\u5bcc\u96c6\u548c\u901a\u8def\u5206\u6790\uff0c\u63ed\u793a\u4e86\u4e0e\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\u53d1\u75c5\u673a\u5236\u76f8\u5173\u7684\u751f\u7269\u5b66\u8fc7\u7a0b\u548c\u4fe1\u53f7\u901a\u8def\uff1b<\/span><\/span><\/p>\n

2\uff0c<\/span>\u7efc\u5408\u5229\u7528\u591a\u79cd\u751f\u7269\u4fe1\u606f\u65b9\u6cd5\u5206\u6790<\/span><\/span>\uff1a<\/span>\u6587\u7ae0\u6587\u7ae0\u5229\u7528\u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\u7f51\u7edc\u6784\u5efa\u548c\u6a21\u5757\u5206\u6790\u63ed\u793a\u4e86\u6838\u5fc3\u7684\u5dee\u5f02\u57fa\u56e0\uff0c\u5e76\u901a\u8fc7\u901a\u8def\u5bcc\u96c6\u5206\u6790\u548cmRNA\u8c03\u63a7\u7f51\u7edc\u5206\u6790\u6df1\u5165\u63a2\u8ba8\u4e86\u8fd9\u4e9b\u57fa\u56e0\u5728\u7ec6\u80de\u8870\u8001\u548c\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\u4e2d\u7684\u529f\u80fd\u7279\u5f81\u3002\u627e\u5230\u4e86\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\u548c\u7ec6\u80de\u8870\u8001\u76f8\u5173\u7684\u57fa\u56e0\u548c\u901a\u8def\u3002\u60f3\u53d1SCI\u7684\u5c0f\u4f19\u4f34\u4eec\u53ef\u4ee5\u501f\u9274\u4e00\u4e0b\u672c\u6587\u7684\u7814\u7a76\u601d\u8def~~<\/span><\/span>(ps\uff1a\u611f\u5174\u8da3\u7684\u540c\u5b66\u6b22\u8fce\u6765\u626b\u7801\u79c1\u804a\u5662\uff01\u5c0f\u4e91<\/span>\u4f5c\u4e3a\u6df1\u8015\u751f\u4fe1\u9886\u57df\u591a\u5e74\u7684\u8001\u751f\u4fe1\u4eba\uff0c\u719f\u6089\u5404\u79cd\u751f\u4fe1\u5206\u6790\u601d\u8def\u548c\u76f8\u5173\u6570\u636e\u5e93\u54e6\uff0c\u603b\u6709\u9002\u5408\u4f60\u7684\u54e6\uff01)\u3002<\/span><\/o:page><\/p>\n

\n
\n
\n
\n
\n
\n
\"\u5929\u5450\uff01\u516c\u5171\u6570\u636e\u5e93\u8fd8\u80fd\u8fd9\u4e48\u7528\uff1f\u6210\u90fd\u533b\u5b66\u9662\u66fe\u660e\u5f3a\u56e2\u961f1\u533a\u65b0\u4f5c\uff01GEO\u6570\u636e\u5e93+CellAge\u6570\u636e\u5e93+\u7efc\u5408\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u5feb\u6765\u5b66\u5440\uff01\"<\/section>\n<\/section>\n<\/section>\n
\n
\n
\u5b9a\u5236\u751f\u4fe1\u5206\u6790<\/strong><\/span><\/p>\n

\u4e91\u670d\u52a1\u5668\u79df\u8d41<\/strong><\/span><\/p>\n

(\u52a0\u5fae\u4fe1\u5907\u6ce899<\/span>\u9886\u53d6\u8bd5\u7528)<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n


\n<\/span><\/p>\n

\"\u5929\u5450\uff01\u516c\u5171\u6570\u636e\u5e93\u8fd8\u80fd\u8fd9\u4e48\u7528\uff1f\u6210\u90fd\u533b\u5b66\u9662\u66fe\u660e\u5f3a\u56e2\u961f1\u533a\u65b0\u4f5c\uff01GEO\u6570\u636e\u5e93+CellAge\u6570\u636e\u5e93+\u7efc\u5408\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u5feb\u6765\u5b66\u5440\uff01\"<\/span><\/p>\n

\u9898\u76ee\uff1a\u901a\u8fc7\u7efc\u5408\u8f6c\u5f55\u7ec4\u5206\u6790\u9274\u5b9a\u4e0e\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\u7ec6\u80de\u8870\u8001\u76f8\u5173\u7684\u4e2d\u5fc3\u57fa\u56e0\u548c\u901a\u8def<\/span><\/p>\n

\u6742\u5fd7\uff1aJournal of Cellular and Molecular Medicine<\/span><\/p>\n

\u5f71\u54cd\u56e0\u5b50\uff1aIF=5.295<\/span><\/p>\n

\u53d1\u8868\u65f6\u95f4\uff1a2023\u5e744\u6708<\/span><\/p>\n

\u7814\u7a76\u80cc\u666f<\/span><\/strong><\/span><\/p>\n

\u968f\u7740\u5168\u7403\u7cd6\u5c3f\u75c5\u60a3\u8005\u6570\u91cf\u7684\u589e\u52a0\uff0c\u7cd6\u5c3f\u75c5\u8db3\u5e76\u53d1\u75c7\u7684\u53d1\u751f\u4e5f\u5728\u589e\u52a0\u3002\u636e\u7edf\u8ba1\uff0c\u5927\u7ea680%\u7684\u4e0b\u80a2\u622a\u80a2\u60a3\u8005\u5728\u624b\u672f\u524d\u90fd\u4f1a\u60a3\u6709\u7cd6\u5c3f\u75c5\u8db3\u3002\u7cd6\u5c3f\u75c5\u8db3\u5e76\u53d1\u75c7\u7684\u75c5\u7406\u673a\u5236\u6d89\u53ca\u5185\u6e90\u6027\u56e0\u7d20\uff0c\u5982\u795e\u7ecf\u3001\u8840\u7ba1\u3001\u514d\u75ab\u3001\u4ee3\u8c22\u548c\u6162\u6027\u611f\u67d3\u7684\u6539\u53d8\uff0c\u4ee5\u53ca\u8fc7\u5ea6\u708e\u75c7\u548c\u8868\u76ae\u6216\u771f\u76ae\u7ec6\u80de\u5bf9\u4fee\u590d\u4fe1\u53f7\u7684\u4e0d\u54cd\u5e94\u3002\u8fd1\u5e74\u6765\uff0c\u8d8a\u6765\u8d8a\u591a\u7684\u8bc1\u636e\u8868\u660e\u7ec6\u80de\u8870\u8001\uff08CS\uff09\u4e0e\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\u7684\u53d1\u751f\u548c\u8fdb\u5c55\u5bc6\u5207\u76f8\u5173\u3002\u6700\u8fd1\u7684\u7814\u7a76\u8868\u660e\uff0c\u9ad8\u80f0\u5c9b\u7d20\u8840\u75c7\u5bfc\u81f4\u6210\u719f\u8102\u80aa\u7ec6\u80de\u4e2d\u65e9\u671f\u8870\u8001\u7684\u8f6c\u5f55\u7ec4\u548c\u5206\u6ccc\u7269\u8d28\u8f6e\u5ed3\uff0c\u540c\u65f6\u5bfc\u81f4\u795e\u7ecf\u5143\u4e2d\u7684\u80f0\u5c9b\u7d20\u62b5\u6297\uff0c\u4ece\u800c\u4ea7\u751f\u8870\u8001\u6837\u8868\u578b\u3002\u56e0\u6b64\u7814\u7a76\u7ec6\u80de\u8870\u8001\u4e0e\u4e0e\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\u7684\u53d1\u751f\u5173\u7cfb\uff0c\u80fd\u591f\u5728\u57fa\u56e0\u6c34\u5e73\u4e0a\u63d0\u4f9b\u5bf9\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\u53d1\u5c55\u7684\u65b0\u89c1\u89e3\uff0c\u80fd\u591f\u4e3a\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\u7684\u65b0\u6cbb\u7597\u7b56\u7565\u3002<\/span><\/p>\n

\u6570\u636e\u6765\u6e90<\/span><\/strong><\/span><\/p>\n\n\n\n\n
\n

\u6570\u636e\u96c6\/\u961f\u5217<\/span><\/p>\n<\/td>\n

\n

\u6570\u636e\u5e93<\/span><\/p>\n<\/td>\n

\n

\u6570\u636e\u7c7b\u578b<\/span><\/p>\n<\/td>\n

\n

\u8be6\u7ec6\u4fe1\u606f<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

GSE80178<\/span><\/p>\n<\/td>\n

\n

GEO<\/span><\/p>\n<\/td>\n

\n

\u57fa\u56e0\u8868\u8fbe\u6570\u636e<\/span><\/p>\n<\/td>\n

\n

\u5305\u542b\u4e86\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\uff08DFU\uff09\u548c\u7cd6\u5c3f\u75c5\u8db3\u76ae\u80a4\uff08DFS\uff09\u6837\u672c<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7814\u7a76\u5229\u7528\u4e86CellAge\u6570\u636e\u5e93\u3002CellAge\u6570\u636e\u5e93\u662f\u4e00\u4e2a\u4e13\u6ce8\u4e8e\u7ec6\u80de\u8870\u8001\u76f8\u5173\u57fa\u56e0\u7684\u8d44\u6e90\u5e93\uff0c\u5176\u4e2d\u6536\u96c6\u4e86\u5404\u79cd\u4eba\u7c7b\u7ec6\u80de\u7c7b\u578b\u7684\u57fa\u56e0\u64cd\u7eb5\u5b9e\u9a8c\u5f97\u5230\u7684\u76f8\u5173\u57fa\u56e0\u4fe1\u606f\u3002\u901a\u8fc7CellAge\u6570\u636e\u5e93\uff0c\u7814\u7a76\u4eba\u5458\u83b7\u53d6\u4e86\u4e0e\u7ec6\u80de\u8870\u8001\u76f8\u5173\u7684279\u4e2a\u57fa\u56e0\u3002<\/span><\/p>\n

\u7814\u7a76\u601d\u8def<\/span><\/strong><\/span><\/p>\n

\u7814\u7a76\u56e2\u961f\u901a\u8fc7\u6574\u5408\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\uff0c\u63a2\u7d22\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\uff08DFU\uff09\u4e2d\u7ec6\u80de\u8870\u8001\uff08CS\uff09\u76f8\u5173\u7684\u57fa\u56e0\u548c\u901a\u8def\u3002\u9996\u5148\uff0c\u6536\u96c6\u5e76\u5206\u6790\u516c\u5171\u6570\u636e\u5e93\u4e2dDFU\u548c\u6b63\u5e38\u76ae\u80a4\u6837\u672c\u7684\u57fa\u56e0\u8868\u8fbe\u6570\u636e\uff0c<\/span>\u8bc6\u522b\u51fa\u4e0eCS\u76f8\u5173\u7684\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\uff08DEGs\uff09\u3002\u7136\u540e\uff0c\u6784\u5efa\u8fd9\u4e9bDEGs\u7684\u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\uff08PPI\uff09\u7f51\u7edc\uff0c\u5e76\u8bc6\u522b\u51fa\u6838\u5fc3\u7684CS-DEGs\u3002\u63a5\u4e0b\u6765\uff0c\u901a\u8fc7\u529f\u80fd\u5bcc\u96c6\u5206\u6790\u548c\u8def\u5f84\u901a\u8def\u5206\u6790\u63ed\u793a\u8fd9\u4e9b\u57fa\u56e0\u5728\u751f\u7269\u5b66\u8fc7\u7a0b\u548c\u4fe1\u53f7\u901a\u8def\u4e2d\u7684\u529f\u80fd\u548c\u4f5c\u7528\u673a\u5236\u3002\u6700\u540e\uff0c\u7ed3\u5408miRNA-TF-mRNA\u8c03\u63a7\u7f51\u7edc\u5206\u6790\uff0c<\/span>\u63a2\u7a76\u8fd9\u4e9bCS-DEGs\u7684\u8c03\u63a7\u7f51\u7edc\u53ca\u5176\u5728DFU\u4e2d\u7684\u8c03\u63a7\u4f5c\u7528\u3002\u8fd9\u4e00\u7814\u7a76\u601d\u8def\u65e8\u5728\u63ed\u793aCS\u4e0eDFU\u53d1\u75c5\u673a\u5236\u4e4b\u95f4\u7684\u5173\u7cfb\u3002<\/span><\/span><\/o:page><\/p>\n

\"\u5929\u5450\uff01\u516c\u5171\u6570\u636e\u5e93\u8fd8\u80fd\u8fd9\u4e48\u7528\uff1f\u6210\u90fd\u533b\u5b66\u9662\u66fe\u660e\u5f3a\u56e2\u961f1\u533a\u65b0\u4f5c\uff01GEO\u6570\u636e\u5e93+CellAge\u6570\u636e\u5e93+\u7efc\u5408\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u5feb\u6765\u5b66\u5440\uff01\"<\/span><\/p>\n

\u7814\u7a76\u7ed3\u679c<\/span><\/strong><\/span><\/p>\n

1.\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\uff08DEGs\uff09\u7684\u8bc6\u522b<\/span><\/p>\n

\u7814\u7a76\u4eba\u5458\u4f7f\u7528\u4e86R\u8bed\u8a00\u4e2d\u7684limma\u5305\u8fdb\u884c\u4e86\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\uff08DEGs\uff09\u7684\u8bc6\u522b\u3002<\/span>\u7814\u7a76\u4eba\u5458\u9996\u5148\u8fdb\u884c\u4e86\u6570\u636e\u7684\u5f52\u4e00\u5316\u5904\u7406\uff0c\u7136\u540e\u5bf9GSE80178\u6570\u636e\u96c6\u8fdb\u884c\u5206\u6790\u3002\u5728\u5f52\u4e00\u5316\u540e\u7684\u6570\u636e\u4e2d\uff0c\u5171\u68c0\u6d4b\u5230\u4e863526\u4e2a\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\uff08DEGs\uff09\uff0c\u8fd9\u4e9b\u57fa\u56e0\u5728\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\uff08DFU\uff09\u548c\u975e\u7cd6\u5c3f\u75c5\u8db3\u76ae\u80a4\uff08DFS\uff09\u6837\u672c\u4e2d\u663e\u793a\u51fa\u663e\u8457\u7684\u8868\u8fbe\u5dee\u5f02\u3002\u4e3a\u4e86\u8fdb\u4e00\u6b65\u63a2\u7d22\u4e0e\u7ec6\u80de\u8870\u8001\uff08CS\uff09\u76f8\u5173\u7684\u5dee\u5f02\u57fa\u56e0\uff0c\u7814\u7a76\u4eba\u5458\u5c06GSE80178\u4e2d\u7684DEGs\u4e0eCellAge\u6570\u636e\u5e93\u4e2d\u7684CS\u76f8\u5173\u57fa\u56e0\u8fdb\u884c\u4e86\u6bd4\u5bf9\uff08\u56fe1\uff09\u3002\u901a\u8fc7Venn\u56fe\u7684\u5206\u6790\uff0c\u786e\u5b9a\u4e8666\u4e2a\u540c\u65f6\u5b58\u5728\u4e8eGSE80178\u548cCellAge\u6570\u636e\u5e93\u4e2d\u7684\u91cd\u53e0\u7684CS-DEGs\u3002\u8fd9\u4e9bCS-DEGs\u7684\u8868\u8fbe\u70ed\u56fe\u5c55\u793a\u4e86\u5b83\u4eec\u5728DFU\u548cDFS\u6837\u672c\u4e4b\u95f4\u7684\u8868\u8fbe\u6a21\u5f0f\u5dee\u5f02\uff0c\u80fd\u591f\u6709\u6548\u533a\u5206\u8fd9\u4e24\u79cd\u6837\u672c\u3002\u8868S1\u4e2d\u63d0\u4f9b\u4e86\u8fd9\u4e9b\u91cd\u53e0\u7684CS-DEGs\u7684\u8be6\u7ec6\u4fe1\u606f\uff0c\u5305\u62ec\u57fa\u56e0\u540d\u3001\u8868\u8fbe\u6a21\u5f0f\u7b49\u3002\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\uff0c\u8fd9\u4e9bCS-DEGs\u53ef\u80fd\u5728\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\u7684\u53d1\u751f\u548c\u53d1\u5c55\u4e2d\u53d1\u6325\u7740\u91cd\u8981\u7684\u4f5c\u7528\u3002<\/span><\/span><\/o:page><\/p>\n

\"\u5929\u5450\uff01\u516c\u5171\u6570\u636e\u5e93\u8fd8\u80fd\u8fd9\u4e48\u7528\uff1f\u6210\u90fd\u533b\u5b66\u9662\u66fe\u660e\u5f3a\u56e2\u961f1\u533a\u65b0\u4f5c\uff01GEO\u6570\u636e\u5e93+CellAge\u6570\u636e\u5e93+\u7efc\u5408\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u5feb\u6765\u5b66\u5440\uff01\"<\/span><\/p>\n

\u56fe1 GSE80178\u6570\u636e\u96c6\u7684\u706b\u5c71\u56fe\u4e0e\u5dee\u5f02\u6027\u5206\u6790<\/span><\/p>\n

2.\u529f\u80fd\u6027\u5206\u6790<\/span><\/p>\n

\u7814\u7a76\u4eba\u5458\u4f7f\u7528\u4e86\u57fa\u56e0\u96c6\u5bcc\u96c6\u5206\u6790\uff08GSEA\uff09\u6765\u8bc6\u522b\u4e0e\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\uff08DFU\uff09\u76f8\u5173\u7684\u751f\u7269\u5b66\u8fc7\u7a0b\u548c\u901a\u8def\u3002<\/span>GSEA\u5206\u6790\u663e\u793a\uff0c\u524d\u4e94\u4e2a\u5bcc\u96c6\u9879\u4e3aRNA\u4ee3\u8c22\u3001\u7ec6\u80de\u5468\u671f\u3001\u6709\u4e1d\u5206\u88c2\u7ec6\u80de\u5468\u671f\u3001\u542b\u6709\u5e3d\u7684\u5185\u542b\u5b50\u9884mRNA\u7684\u52a0\u5de5\u4ee5\u53ca\u7ec6\u80de\u5668\u7684\u751f\u6210\u548c\u7ef4\u6301\uff08\u56fe2A\uff09\u3002\u8fd9\u4e9b\u7ed3\u679c\u63d0\u793a\u4e86\u5728DFU\u7684\u53d1\u751f\u548c\u53d1\u5c55\u8fc7\u7a0b\u4e2d\uff0cRNA\u4ee3\u8c22\u3001\u7ec6\u80de\u5468\u671f\u8c03\u63a7\u548c\u7ec6\u80de\u5668\u529f\u80fd\u7ef4\u6301\u7b49\u751f\u7269\u5b66\u8fc7\u7a0b\u53ef\u80fd\u8d77\u7740\u91cd\u8981\u4f5c\u7528\u3002<\/span>\u4e3a\u4e86\u8fdb\u4e00\u6b65\u5206\u6790\u6d89\u53ca\u7ec6\u80de\u8870\u8001\u76f8\u5173\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\uff08CS-DEGs\uff09\u7684\u751f\u7269\u529f\u80fd\u548c\u901a\u8def\uff0c\u7814\u7a76\u4eba\u5458\u8fdb\u884c\u4e86GO\uff08Gene Ontology\uff09\u548cKEGG\uff08Kyoto Encyclopedia of Genes and Genomes\uff09\u901a\u8def\u5bcc\u96c6\u5206\u6790\u3002<\/span>GO\u5206\u6790\u7ed3\u679c\u663e\u793a\uff0cCS-DEGs\u5728\u751f\u7269\u8fc7\u7a0b\uff08BP\uff09\u65b9\u9762\u663e\u8457\u5bcc\u96c6\u4e8e\u7ec6\u80de\u8870\u8001\u3001\u7ec6\u80de\u8001\u5316\u548c\u8870\u8001\u7b49\u8fc7\u7a0b\u3002\u5728\u7ec6\u80de\u7ec4\u5206\uff08CC\uff09\u65b9\u9762\uff0c\u8fd9\u4e9b\u5dee\u5f02\u57fa\u56e0\u4e3b\u8981\u4e0e\u6838\u67d3\u8272\u8d28\u3001PcG\u86cb\u767d\u590d\u5408\u7269\u548c\u8f6c\u5f55\u6291\u5236\u590d\u5408\u7269\u76f8\u5173\uff0c\u6697\u793a\u4e86\u8fd9\u4e9b\u57fa\u56e0\u5728\u7ec6\u80de\u6838\u5185\u7684\u529f\u80fd\u6d3b\u52a8\u3002\u5728\u5206\u5b50\u529f\u80fd\uff08MF\uff09\u65b9\u9762\uff0cCS-DEGs\u4e3b\u8981\u4e0eMAP\u6fc0\u9176\u6fc0\u9176\u6d3b\u6027\u3001\u86cb\u767d\u916a\u6c28\u9178\u6fc0\u9176\u6d3b\u6027\u548c\u86cb\u767d\u4e1d\u6c28\u9178\/\u82cf\u6c28\u9178\/\u916a\u6c28\u9178\u6fc0\u9176\u6d3b\u6027\u76f8\u5173\uff0c\u8868\u660e\u5b83\u4eec\u53ef\u80fd\u53c2\u4e0e\u4fe1\u53f7\u4f20\u5bfc\u548c\u8c03\u8282\u7ec6\u80de\u7684\u751f\u7269\u5b66\u529f\u80fd\uff08\u56fe3\uff09\u3002KEGG\u901a\u8def\u5206\u6790\u663e\u793a\uff0c\u8fd9\u4e9bCS-DEGs\u4e3b\u8981\u53c2\u4e0e\u7ec6\u80de\u8870\u8001\u3001\u5361\u6ce2\u897f\u8089\u7624\u76f8\u5173\u7684\u5355\u7eaf\u75b1\u75b9\u75c5\u6bd2\u611f\u67d3\u4ee5\u53caToll\u6837\u53d7\u4f53\u4fe1\u53f7\u901a\u8def\u7b49\u901a\u8def\u3002\u8fd9\u4e9b\u7ed3\u679c\u4e3a\u8fdb\u4e00\u6b65\u7814\u7a76\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\u7684\u53d1\u75c5\u673a\u5236\u548c\u6cbb\u7597\u63d0\u4f9b\u4e86\u91cd\u8981\u7ebf\u7d22\uff0c\u63d0\u793a\u7ec6\u80de\u8870\u8001\u53ef\u80fd\u5728DFU\u7684\u53d1\u751f\u548c\u53d1\u5c55\u4e2d\u8d77\u7740\u5173\u952e\u4f5c\u7528\u3002<\/span><\/span><\/o:page><\/p>\n

\"\u5929\u5450\uff01\u516c\u5171\u6570\u636e\u5e93\u8fd8\u80fd\u8fd9\u4e48\u7528\uff1f\u6210\u90fd\u533b\u5b66\u9662\u66fe\u660e\u5f3a\u56e2\u961f1\u533a\u65b0\u4f5c\uff01GEO\u6570\u636e\u5e93+CellAge\u6570\u636e\u5e93+\u7efc\u5408\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u5feb\u6765\u5b66\u5440\uff01\"<\/span><\/p>\n

\u56fe2 \u6570\u636e\u5bcc\u96c6\u9879\u548c\u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\u7f51\u7edc\u53ef\u89c6\u5316\u5206\u6790<\/span>    <\/span><\/o:page><\/p>\n

\"\u5929\u5450\uff01\u516c\u5171\u6570\u636e\u5e93\u8fd8\u80fd\u8fd9\u4e48\u7528\uff1f\u6210\u90fd\u533b\u5b66\u9662\u66fe\u660e\u5f3a\u56e2\u961f1\u533a\u65b0\u4f5c\uff01GEO\u6570\u636e\u5e93+CellAge\u6570\u636e\u5e93+\u7efc\u5408\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u5feb\u6765\u5b66\u5440\uff01\"<\/span><\/p>\n

\u56fe3\u7ec6\u80de\u8870\u8001\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u5bcc\u96c6\u7ed3\u679c\u5206\u6790<\/span><\/p>\n

3. \u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\uff08PPI\uff09\u7f51\u7edc\u5206\u6790<\/span><\/p>\n

\u7814\u7a76\u56e2\u961f\u5229\u7528\u901a\u8fc7Cytoscape\u6784\u5efa\u4e86\u4e0e\u7ec6\u80de\u8870\u8001\u76f8\u5173\u7684\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\uff08CS-DEGs\uff09\u7684\u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\uff08PPI\uff09\u7f51\u7edc\uff0c\u5e76\u8fdb\u884c\u4e86\u6a21\u5757\u5206\u6790\u3002\u9996\u5148\u7814\u7a76\u4eba\u5458\u5229\u7528Cytoscape\u6784\u5efa\u4e86CS-DEGs\u7684PPI\u7f51\u7edc\u3002<\/span>\u7f51\u7edc\u663e\u793a\u4e86CS-DEGs\u4e4b\u95f4\u7684\u590d\u6742\u76f8\u4e92\u4f5c\u7528\u6a21\u5f0f\uff0c\u63ed\u793a\u4e86\u5b83\u4eec\u5728\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\uff08DFU\uff09\u53d1\u751f\u548c\u53d1\u5c55\u4e2d\u53ef\u80fd\u626e\u6f14\u7684\u89d2\u8272\u3002<\/span>\u63a5\u7740\uff0c\u7814\u7a76\u4eba\u5458\u5229\u7528Cytoscape\u7684MCODE\u63d2\u4ef6\u8fdb\u884c\u4e86\u6a21\u5757\u5206\u6790\uff0c\u53d1\u73b0\u4e86\u4e09\u4e2a\u5bc6\u5207\u76f8\u5173\u7684\u57fa\u56e0\u6a21\u5757\uff0c\u5305\u62ec18\u4e2aCS-DEGs\u548c41\u4e2a\u76f8\u4e92\u4f5c\u7528\u5bf9\uff08\u56fe4A\u2013C\uff09\u3002\u8fd9\u4e9b\u6a21\u5757\u53ef\u80fd\u4ee3\u8868\u4e86\u5728DFU\u75c5\u7406\u751f\u7406\u8fc7\u7a0b\u4e2d\u529f\u80fd\u7d27\u5bc6\u76f8\u5173\u7684\u57fa\u56e0\u96c6\u5408\u3002<\/span>GO\uff08Gene Ontology\uff09\u5206\u6790\u663e\u793a\u8fd9\u4e9b\u57fa\u56e0\u53c2\u4e0e\u4e86\u591a\u4e2a\u751f\u7269\u5b66\u8fc7\u7a0b\uff0c\u5305\u62ec\u8282\u5f8b\u8fc7\u7a0b\u3001\u80f0\u5c9b\u7d20\u53d7\u4f53\u4fe1\u53f7\u901a\u8def\u7684\u8c03\u8282\u3001\u8f6c\u79fb\u9176\u590d\u5408\u7269\u3001\u542b\u78f7\u57fa\u56e2\u7684\u8f6c\u79fb\u3001PcG\u86cb\u767d\u590d\u5408\u7269\u3001\u7279\u5f02\u6027\u542f\u52a8\u5b50\u67d3\u8272\u8d28\u7ed3\u5408\u4ee5\u53ca\u86cb\u767dC-\u672b\u7aef\u7ed3\u5408\u7b49\u8fc7\u7a0b\uff08\u89c1\u56fe5D\uff09\u3002\u8fd9\u4e9b\u7ed3\u679c\u63d0\u793a\u4e86\u8fd9\u4e9bCS-DEGs\u53ef\u80fd\u901a\u8fc7\u591a\u79cd\u9014\u5f84\u5f71\u54cdDFU\u7684\u53d1\u751f\u548c\u53d1\u5c55\u3002<\/span><\/span><\/o:page><\/p>\n

\"\u5929\u5450\uff01\u516c\u5171\u6570\u636e\u5e93\u8fd8\u80fd\u8fd9\u4e48\u7528\uff1f\u6210\u90fd\u533b\u5b66\u9662\u66fe\u660e\u5f3a\u56e2\u961f1\u533a\u65b0\u4f5c\uff01GEO\u6570\u636e\u5e93+CellAge\u6570\u636e\u5e93+\u7efc\u5408\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u5feb\u6765\u5b66\u5440\uff01\"<\/span><\/p>\n

\u56fe4 \u663e\u8457\u57fa\u56e0\u805a\u7c7b\u6a21\u5757\u53ca\u5176\u5bcc\u96c6\u5206\u6790<\/span><\/p>\n

4.\u6838\u5fc3CS-DEGs\u7684\u9009\u62e9\u548c\u5206\u6790<\/span><\/p>\n

\u7814\u7a76\u4eba\u5458\u8ba1\u7b97\u4e86\u524d10\u4e2a\u67a2\u7ebd\u57fa\u56e0\uff0c\u53d1\u73b0\u4e868\u4e2a\u91cd\u53e0\u7684\u67a2\u7ebd\u57fa\u56e0\uff0c\u5206\u522b\u662fTP53\u3001SRC\u3001SIRT1\u3001CCND1\u3001EZH2\u3001CXCL8\u3001AR\u548cCDK4\uff08\u56fe5A\uff09\u3002\u63a5\u7740\uff0c<\/span>\u7814\u7a76\u4eba\u5458\u5229\u7528GeneMANIA\u6570\u636e\u5e93\u8fdb\u884c\u4e86\u8fd9\u4e9b\u57fa\u56e0\u7684\u5171\u8868\u8fbe\u7f51\u7edc\u548c\u76f8\u5173\u529f\u80fd\u7684\u5206\u6790\u3002<\/span>\u8fd9\u4e9b\u57fa\u56e0\u5c55\u793a\u4e86\u4e00\u4e2a\u590d\u6742\u7684\u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\u7f51\u7edc\uff08PPI\u7f51\u7edc\uff09\uff0c\u5176\u4e2d\u7269\u7406\u76f8\u4e92\u4f5c\u7528\u536044.88%\uff0c\u9884\u6d4b\u76f8\u4e92\u4f5c\u7528\u536019.39%\uff0c\u5171\u5b9a\u4f4d\u536012.51%\uff0c\u901a\u8def\u536011.63%\uff0c\u9057\u4f20\u76f8\u4e92\u4f5c\u7528\u53606.28%\uff0c\u5171\u8868\u8fbe\u53605.32%\uff08\u56fe5B\uff09\u3002\u8fd9\u4e9b\u57fa\u56e0\u4e0e\u8c03\u8282\u7ec6\u80de\u5468\u671fG1\/S\u671f\u8f6c\u6362\u3001\u6709\u4e1d\u5206\u88c2\u7ec6\u80de\u5468\u671fG1\/S\u8f6c\u6362\u4ee5\u53ca\u6709\u4e1d\u5206\u88c2\u7ec6\u80de\u5468\u671fG1\/S\u8f6c\u6362\u76f8\u5173\u3002GO\u5206\u6790\u663e\u793a\uff0c\u8fd9\u4e9b\u57fa\u56e0\u4e0e\u5bf9\u916e\u7684\u54cd\u5e94\u3001\u7ec6\u80de\u5bf9\u916e\u7684\u53cd\u5e94\u3001\u6838\u67d3\u8272\u8d28\u3001ESC\/E(Z)\u590d\u5408\u7269\u3001RNA\u805a\u5408\u9176II\u57fa\u7840\u8f6c\u5f55\u56e0\u5b50\uff08TF\uff09\u7ed3\u5408\u548c\u8f6c\u5f55\u5171\u6291\u5236\u6d3b\u6027\u76f8\u5173\u3002KEGG\u901a\u8def\u5206\u6790\u53d1\u73b0\uff0c\u8fd9\u4e9b\u57fa\u56e0\u4e3b\u8981\u4e0e\u8180\u80f1\u764c\u3001\u7ec6\u80de\u8870\u8001\u4ee5\u53ca\u4e0e\u5361\u6ce2\u897f\u8089\u7624\u76f8\u5173\u7684\u5355\u7eaf\u75b1\u75b9\u75c5\u6bd2\u611f\u67d3\u76f8\u5173\u3002<\/span><\/span><\/o:page><\/p>\n

\"\u5929\u5450\uff01\u516c\u5171\u6570\u636e\u5e93\u8fd8\u80fd\u8fd9\u4e48\u7528\uff1f\u6210\u90fd\u533b\u5b66\u9662\u66fe\u660e\u5f3a\u56e2\u961f1\u533a\u65b0\u4f5c\uff01GEO\u6570\u636e\u5e93+CellAge\u6570\u636e\u5e93+\u7efc\u5408\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u5feb\u6765\u5b66\u5440\uff01\"<\/span><\/p>\n

\u56fe5 \u7ec6\u80de\u8870\u8001\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u53ca\u6838\u5fc3\u57fa\u56e0\u7684\u5173\u8054\u5206\u6790<\/span><\/p>\n

5. miRNA-TF-mRNA\u8c03\u63a7\u7f51\u7edc\u5206\u6790<\/span><\/p>\n

\u7814\u7a76\u56e2\u961f\u8fdb\u884c\u4e86miRNA-TF-mRNA\u8c03\u63a7\u7f51\u7edc\u7684\u5206\u6790\u3002\u5229\u7528TRRUST\u6570\u636e\u5e93\u7814\u7a76\u4eba\u5458\u53d1\u73b0\u4e863\u4e2aCS-DEGs\u5145\u5f53\u8f6c\u5f55\u56e0\u5b50\uff0c\u4ee5\u8c03\u63a7\u5176\u4ed6\u57fa\u56e0\u7684\u8868\u8fbe\u3002<\/span>\u8fd9\u4e9bCS-DEGs\u5305\u62ecTP53\uff08\u8c03\u63a7EZH2\u3001TP53\u3001CDK4\u3001CCND1\uff09\u3001SIRT1\uff08\u8c03\u63a7CCND1\u3001TP53\u3001EZH2\uff09\u548cEZH2\uff08\u8c03\u63a7CCND1\u3001TP53\uff09\u3002\u7814\u7a76\u4eba\u5458\u4f7f\u7528GSE84971\u6570\u636e\u96c6\u83b7\u5f97\u4e8631\u4e2a\u5dee\u5f02\u8868\u8fbe\u7684miRNA\u3002\u7136\u540e\uff0c\u6211\u4eec\u5229\u7528miRWalk\u6570\u636e\u5e93\u5206\u522b\u4eceTargetScan\u3001miRDB\u548cmiRTarBase\u9884\u6d4b\u4e86\u8fd98\u4e2a\u57fa\u56e0\u7684\u4e0a\u6e38miRNA\uff0c\u9884\u6d4b\u5f97\u5230\u7684\u4e0a\u6e38miRNA\u5206\u522b\u4e3a127\u30011070\u548c928\u3002\u7ecf\u8fc7Venn\u56fe\u7684\u4ea4\u96c6\u5904\u7406\uff0c\u83b7\u5f97\u4e863\u4e2a\u91cd\u53e0\u7684miRNA\uff0c\u5305\u62echsa-miR-93-5p\uff08\u4f5c\u7528\u4e8eCCND1\uff09\u3001hsa-miR-15a-5p\uff08\u4f5c\u7528\u4e8eCCND1\uff09\u548chsa-miR-132-3p\uff08\u4f5c\u7528\u4e8eSIRT1\uff09\u3002<\/span>\u6700\u540e\uff0c\u7814\u7a76\u4eba\u5458\u5229\u7528Cytoscape\u6784\u5efa\u4e86miRNA-TF-mRNA\u8c03\u63a7\u7f51\u7edc\u3002<\/span>SIRT1\u4f5c\u4e3a\u8f6c\u5f55\u56e0\u5b50\u53d7\u5230\u4e0a\u6e38hsa-miR-132-3p\u7684\u8c03\u63a7\uff0c\u5e76\u540c\u65f6\u8c03\u63a7\u4e0b\u6e38\u7684CS-DEGs\u7684\u8868\u8fbe\u3002<\/span><\/span><\/o:page><\/p>\n

\"\u5929\u5450\uff01\u516c\u5171\u6570\u636e\u5e93\u8fd8\u80fd\u8fd9\u4e48\u7528\uff1f\u6210\u90fd\u533b\u5b66\u9662\u66fe\u660e\u5f3a\u56e2\u961f1\u533a\u65b0\u4f5c\uff01GEO\u6570\u636e\u5e93+CellAge\u6570\u636e\u5e93+\u7efc\u5408\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u5feb\u6765\u5b66\u5440\uff01\"<\/span><\/p>\n

\u56fe6 miRNA-TF-mRNA\u8c03\u63a7\u7f51\u7edc\u5206\u6790<\/span><\/p>\n

\u6587\u7ae0\u5c0f\u7ed3<\/span><\/strong><\/span><\/p>\n

\u8fd9\u7bc7\u6587\u7ae0\u8fd9\u7bc7\u6587\u7ae0\u6df1\u5ea6\u7ed3\u5408\u4e86\u4e24\u4e2a\u516c\u5f00\u6570\u636e\u5e93\uff0c\u53d1\u73b0\u4e86\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\u548c\u7ec6\u80de\u8870\u8001\u76f8\u5173\u7684\u57fa\u56e0\u548c\u901a\u8def\u3002\u5e76\u901a\u8fc7\u901a\u8def\u5bcc\u96c6\u5206\u6790\u548cmRNA\u8c03\u63a7\u7f51\u7edc\u5206\u6790\u6df1\u5165\u63a2\u8ba8\u4e86\u8fd9\u4e9b\u57fa\u56e0\u5728\u7ec6\u80de\u8870\u8001\u548c\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\u4e2d\u7684\u529f\u80fd\u7279\u5f81\uff0c\u63ed\u793a\u4e86\u4e0e\u7cd6\u5c3f\u75c5\u8db3\u6e83\u75a1\u53d1\u75c5\u673a\u5236\u76f8\u5173\u7684\u751f\u7269\u5b66\u8fc7\u7a0b\u548c\u4fe1\u53f7\u901a\u8def\u3002<\/span>\u770b\u5230\u8fd9\u4e0d\u77e5\u9053\u5c0f\u4f19\u4f34\u4eec\u6709\u6ca1\u6709\u60f3\u6cd5\uff1f\u628a\u63e1\u4f4f\u751f\u4fe1\u5206\u6790\u601d\u8def\u6d41\u7a0b\uff0c\u6587\u7ae0\u5feb\u5feb\u53d1\u8d77\u6765\u5427\uff01\u611f\u5174\u8da3\u7684\u8bdd\uff0c<\/span><\/span><\/strong><\/span>\u6709\u9700\u8981\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u626b\u7801\u6765\u627e\u5c0f\u4e91\u505a\u8bbe\u8ba1\u54df<\/span>~<\/span><\/span><\/p>\n

\n
\n
\n
\n
\n
\n
\"\u5929\u5450\uff01\u516c\u5171\u6570\u636e\u5e93\u8fd8\u80fd\u8fd9\u4e48\u7528\uff1f\u6210\u90fd\u533b\u5b66\u9662\u66fe\u660e\u5f3a\u56e2\u961f1\u533a\u65b0\u4f5c\uff01GEO\u6570\u636e\u5e93+CellAge\u6570\u636e\u5e93+\u7efc\u5408\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u5feb\u6765\u5b66\u5440\uff01\"<\/section>\n<\/section>\n<\/section>\n
\n
\n
\n

\u5b9a\u5236\u751f\u4fe1\u5206\u6790<\/span><\/strong><\/span><\/p>\n

\n

\u4e91\u670d\u52a1\u5668\u79df\u8d41<\/strong><\/span>\n<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

\n
\n
<\/section>\n<\/section>\n<\/section>\n
\n
\n
\n
\n

\u70ed\u70b9\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

\n
\n
\n
\n
\n
\u5b5f\u5fb7\u5c14\u968f\u673a\u5316<\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n
\n
\n
\n
\n
\u4e34\u5e8a\u516c\u5171\u6570\u636e\u5206\u6790<\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n
\n
\n
\n
\n
\n
\u5355\u7ec6\u80de\u6d4b\u5e8f<\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n
\n
\n
\n
\n
\u80bf\u7624\u514d\u75ab\u4e0e\u5fae\u73af\u5883<\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n
\n
\n
\n
\n
\n
\u673a\u5668\u5b66\u4e60<\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n
\n
\n
\n
\n
\u5355\u57fa\u56e0\u5206\u6790<\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n
\n
\n
\n
\n

\u751f\u4fe1\u4e91\u670d\u52a1\u5668<\/strong><\/span><\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

\n
\n
\n
\n

\u4ee3\u7801\u5408\u96c6<\/strong><\/span><\/a>\uff08\u70b9\u51fb\u67e5\u770b\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

\n
\n
\n
\n

\u57f9\u8bad\u73ed\u7cfb\u5217<\/span><\/strong>\uff08\u70b9\u51fb\u67e5\u770b\uff09<\/span><\/span><\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

\n
<\/mp-style-type><\/section>\n<\/section>\n

\u539f\u6587\u59cb\u53d1\u4e8e\u5fae\u4fe1\u516c\u4f17\u53f7\uff08\u4e91\u751f\u4fe1\u5b66\u751f\u7269\u4fe1\u606f\u5b66\uff09\uff1a\u5929\u5450\uff01\u516c\u5171\u6570\u636e\u5e93\u8fd8\u80fd\u8fd9\u4e48\u7528\uff1f\u6210\u90fd\u533b\u5b66\u9662\u66fe\u660e\u5f3a\u56e2\u961f1\u533a\u65b0\u4f5c\uff01GEO\u6570\u636e\u5e93+CellAge\u6570\u636e\u5e93+\u7efc\u5408\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u5feb\u6765\u5b66\u5440\uff01<\/a><\/p><\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

\u6628\u5929\u6709\u5c0f\u4f19\u4f34\u79c1\u4fe1\u5c0f\u4e91\uff0c\u89c9\u5f97\u516c\u5171\u6570\u636e\u5e93\u4e2d\u6570\u636e\u96c6\u5f80\u5f80\u7ecf\u8fc7\u5927\u91cf\u5206\u6790\uff0c\u96be\u4ee5\u521b\u65b0\u3002\u6709\u4e00\u5b9a\u9053\u7406\uff0c\u4f46\u53ea\u8981\u7528\u5bf9\u601d\u8def\uff0c\u7efc\u5408\u5229\u7528\u516c […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/43961"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=43961"}],"version-history":[{"count":2,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/43961\/revisions"}],"predecessor-version":[{"id":44112,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/43961\/revisions\/44112"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=43961"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=43961"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=43961"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}