{"id":44011,"date":"2024-06-02T01:20:52","date_gmt":"2024-06-01T17:20:52","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=44011"},"modified":"2024-06-02T17:28:21","modified_gmt":"2024-06-02T09:28:21","slug":"b5556740a4","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=44011","title":{"rendered":"\u201c\u5e72\u6e7f\u7ed3\u5408\u201d\u53d124\u5206\u6587\u7ae0\uff0c\u6027\u4ef7\u6bd4MAX\uff01\u5357\u65b9\u533b\u79d1\u5927\u5b66\u8303\u5e94\u65b9\u7b49\u56e2\u961f\u6559\u4f60\u751f\u4fe1\u4e0e\u5b9e\u9a8c\u65e0\u7f1d\u5bf9\u63a5\uff0c\u5c55\u73b0\u79d1\u7814\u65e0\u9650\u53ef\u80fd\uff01"},"content":{"rendered":"
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\u200d<\/span><\/p>\n

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\u5b9a\u5236\u751f\u4fe1\u5206\u6790<\/strong><\/span><\/p>\n

\u4e91\u670d\u52a1\u5668\u79df\u8d41<\/strong><\/span><\/p>\n

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\u00a0<\/span><\/p>\n

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\u6742\u5fd7\uff1aDrug Resistance Updates<\/span><\/span><\/p>\n

\u5f71\u54cd\u56e0\u5b50\uff1aIF=24.3<\/span><\/span><\/p>\n

\u53d1\u8868\u65f6\u95f4\uff1a2024\u5e743\u6708<\/span><\/span><\/p>\n

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\u7814\u7a76\u80cc\u666f<\/span><\/p>\n

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\u7814\u7a76\u601d\u8def<\/span><\/p>\n

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\u4e3b\u8981\u7ed3\u679c<\/span><\/p>\n

1.TRIM29\u8fc7\u8868\u8fbe\u4e0ePDAC\u60a3\u8005\u9884\u540e\u4e0d\u826f\u76f8\u5173<\/span> \u00a0 \u00a0<\/span><\/p>\n

\u9996\u5148\uff0c\u901a\u8fc7\u5206\u6790GEO\u6570\u636e\u5e93\u4e2d\u7684\u4e09\u4e2a\u72ec\u7acb\u6570\u636e\u96c6\uff08GSE15471\uff0cGSE16515\uff0cGSE62452\uff09\uff0c\u7814\u7a76\u8005\u53d1\u73b0<\/span>TRIM29\u5728PDAC\u7ec4\u7ec7\u4e2d\u7684\u8868\u8fbe\u6c34\u5e73\u663e\u8457\u9ad8\u4e8e\u975e\u80bf\u7624\u7ec4\u7ec7<\/span>\uff08\u56fe1A-C\uff09\u3002\u6b64\u5916\uff0c\u5229\u7528TCGA\u6570\u636e\u5e93\u4e2d\u7684\u4e34\u5e8a\u4fe1\u606f\u8fdb\u884c\u751f\u5b58\u5206\u6790\u663e\u793a\uff0c<\/span>\u9ad8TRIM29\u8868\u8fbe\u4e0ePDAC\u60a3\u8005\u8f83\u5dee\u7684\u9884\u540e\u5bc6\u5207\u76f8\u5173<\/span>\uff08\u56fe1D\uff09\u3002\u4e3a\u4e86\u8fdb\u4e00\u6b65\u9a8c\u8bc1\u8fd9\u4e9b\u7ed3\u679c\uff0c\u7814\u7a76\u8005\u901a\u8fc7\u514d\u75ab\u7ec4\u5316\u5206\u6790\u5728\u7ec4\u7ec7\u82af\u7247\uff08<\/span>TMA<\/span>\uff09\u4e0a\u8bc4\u4f30\u4e86TRIM29\u86cb\u767d\u7684\u8868\u8fbe\uff0c\u7ed3\u679c\u540c\u6837\u8868\u660e<\/span>TRIM29\u5728PDAC\u7ec4\u7ec7\u4e2d\u8868\u8fbe\u4e0a\u8c03<\/span>\uff08\u56fe1E\uff09\u3002\u8fd9\u4e9b\u7ed3\u679c\u5171\u540c\u652f\u6301\u4e86<\/span>TRIM29\u4f5c\u4e3aPDAC\u6f5c\u5728\u751f\u7269\u6807\u5fd7\u7269<\/span>\u7684\u53ef\u80fd\u6027\uff0c\u5e76\u53ef\u80fd\u4e0e\u5176\u5bf9GEM\u7684\u62b5\u6297\u6027\u6709\u5173\u3002<\/span><\/span><\/p>\n

\"\u201c\u5e72\u6e7f\u7ed3\u5408\u201d\u53d124\u5206\u6587\u7ae0\uff0c\u6027\u4ef7\u6bd4MAX\uff01\u5357\u65b9\u533b\u79d1\u5927\u5b66\u8303\u5e94\u65b9\u7b49\u56e2\u961f\u6559\u4f60\u751f\u4fe1\u4e0e\u5b9e\u9a8c\u65e0\u7f1d\u5bf9\u63a5\uff0c\u5c55\u73b0\u79d1\u7814\u65e0\u9650\u53ef\u80fd\uff01\"<\/span><\/p>\n

2.TRIM29\u4e0eGEM\u62b5\u6297\u6027\u76f8\u5173<\/span><\/p>\n

\u901a\u8fc7\u6bd4\u8f83PDAC\u7ec6\u80de\u682aSW1990\u548cPANC-1\u53ca\u5176\u5bf9\u5e94\u7684\u8010\u836f\u682aSW1990-GR\u548cPANC-1-GR\uff0c\u7814\u7a76\u8005\u53d1\u73b0<\/span>\u8010\u836f\u682a\u5bf9GEM\u7684\u654f\u611f\u6027\u663e\u8457\u964d\u4f4e<\/span>\uff0c\u8868\u73b0\u4e3aGEM\u7684\u534a\u6570\u6291\u5236\u6d53\u5ea6\uff08IC50\uff09\u663e\u8457\u589e\u52a0\uff08\u56fe2E\u548cF\uff09\u3002\u6b64\u5916\uff0c\u901a\u8fc7siRNA\u6280\u672f\u6572\u4f4eTRIM29\u7684\u8868\u8fbe\u53ef\u4ee5\u964d\u4f4e\u8010\u836f\u682a\u7684IC50\u503c\uff0c<\/span>\u800cTRIM29\u7684\u8fc7\u8868\u8fbe\u5219\u589e\u52a0\u4e86IC50\u503c<\/span>\uff08\u56fe2G\u548cH\uff09\u3002\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e<\/span>TRIM29\u7684\u8868\u8fbe\u6c34\u5e73\u4e0ePDAC\u7ec6\u80de\u5bf9GEM\u7684\u62b5\u6297\u6027\u5bc6\u5207\u76f8\u5173<\/span>\uff0c\u63d0\u793aTRIM29\u53ef\u80fd\u901a\u8fc7\u8c03\u8282\u76f8\u5173\u4fe1\u53f7\u901a\u8def\u6765\u5f71\u54cdPDAC\u7684\u836f\u7269\u6cbb\u7597\u53cd\u5e94\u3002<\/span><\/span><\/p>\n

\"\u201c\u5e72\u6e7f\u7ed3\u5408\u201d\u53d124\u5206\u6587\u7ae0\uff0c\u6027\u4ef7\u6bd4MAX\uff01\u5357\u65b9\u533b\u79d1\u5927\u5b66\u8303\u5e94\u65b9\u7b49\u56e2\u961f\u6559\u4f60\u751f\u4fe1\u4e0e\u5b9e\u9a8c\u65e0\u7f1d\u5bf9\u63a5\uff0c\u5c55\u73b0\u79d1\u7814\u65e0\u9650\u53ef\u80fd\uff01\"<\/span> \u00a0 \u00a0<\/span><\/p>\n

3. TRIM29\u6fc0\u6d3bMEK\/ERK\u4fe1\u53f7\u901a\u8def<\/span><\/p>\n

GSEA\u5206\u6790\u663e\u793a\uff0c<\/span>TRIM29\u8fc7\u8868\u8fbe\u4e0eMEK\/ERK\u4fe1\u53f7\u901a\u8def\u7684\u57fa\u56e0\u7b7e\u540d\u76f8\u5173<\/span>\uff08\u56fe3A-D\uff09\u3002\u63a5\u7740\uff0c\u86cb\u767d\u514d\u75ab\u5370\u8ff9\u5206\u6790\u53d1\u73b0<\/span>TRIM29\u7684\u8fc7\u8868\u8fbe\u663e\u8457\u589e\u52a0\u4e86MEK\u548cERK\u7684\u78f7\u9178\u5316\u6c34\u5e73<\/span>\uff08\u5373\u6fc0\u6d3b\u5f62\u5f0f\u7684pMEK\u548cpERK\uff09\uff0c\u800cTRIM29\u7684<\/span>\u6572\u4f4e\u5219\u964d\u4f4e\u4e86\u8fd9\u4e9b\u86cb\u767d\u7684\u78f7\u9178\u5316\u6c34\u5e73<\/span>\uff08\u56fe3E\u548cF\uff09\u3002\u8fd9\u4e00\u53d1\u73b0\u8868\u660eTRIM29\u53ef\u80fd\u901a\u8fc7\u8c03\u8282MEK\/ERK\u4fe1\u53f7\u901a\u8def\u7684\u6d3b\u6027\u6765\u5f71\u54cdPDAC\u7ec6\u80de\u7684\u751f\u7269\u5b66\u884c\u4e3a\u3002<\/span>\u7531\u4e8eMEK\/ERK\u4fe1\u53f7\u901a\u8def\u5728\u8c03\u63a7\u7ec6\u80de\u589e\u6b96\u3001\u5b58\u6d3b\u548c\u836f\u7269\u62b5\u6297\u6027\u4e2d\u626e\u6f14\u7740\u5173\u952e\u89d2\u8272\uff0cTRIM29\u5bf9\u8fd9\u4e00\u901a\u8def\u7684\u6fc0\u6d3b\u53ef\u80fd\u662f\u5176\u4fc3\u8fdbGEM\u62b5\u6297\u6027\u7684\u5206\u5b50\u673a\u5236\u4e4b\u4e00\u3002<\/span><\/span><\/p>\n

\"\u201c\u5e72\u6e7f\u7ed3\u5408\u201d\u53d124\u5206\u6587\u7ae0\uff0c\u6027\u4ef7\u6bd4MAX\uff01\u5357\u65b9\u533b\u79d1\u5927\u5b66\u8303\u5e94\u65b9\u7b49\u56e2\u961f\u6559\u4f60\u751f\u4fe1\u4e0e\u5b9e\u9a8c\u65e0\u7f1d\u5bf9\u63a5\uff0c\u5c55\u73b0\u79d1\u7814\u65e0\u9650\u53ef\u80fd\uff01\"<\/span><\/p>\n

4. circRPS29\/miR-770\u20135p\/TIM29\u8f74\u7684\u9884\u6d4b<\/span><\/p>\n

\u901a\u8fc7<\/span>\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u548c\u5b9e\u9a8c\u9a8c\u8bc1<\/span>\uff0c\u9884\u6d4b\u5e76\u63a2\u7d22\u4e86<\/span>circRPS29\u3001miR-770\u20135p\u548cTRIM29\u4e4b\u95f4\u7684\u8c03\u63a7\u5173\u7cfb<\/span>\u3002\u7814\u7a76\u8005\u53d1\u73b0miR-770\u20135p\u5728PDAC\u7ec4\u7ec7\u4e2d\u8868\u8fbe\u4e0b\u8c03\uff0c\u5e76\u4e14\u4e0eTRIM29\u7684\u8868\u8fbe\u6c34\u5e73\u5448\u8d1f\u76f8\u5173\uff08\u56fe4A\u548cB\uff09\u3002\u6b64\u5916\uff0c\u4e34\u5e8a\u6570\u636e\u8868\u660emiR-770\u20135p\u7684\u4f4e\u8868\u8fbe\u4e0ePDAC\u60a3\u8005\u8f83\u5dee\u7684\u603b\u4f53\u751f\u5b58\u7387\u76f8\u5173\uff08\u56fe4C\uff09\u3002\u901a\u8fc7ENCORI\u5e73\u53f0\u7684\u5206\u6790\uff0c\u7814\u7a76\u8005\u9884\u6d4b\u4e86circRPS29\u53ef\u80fd\u901a\u8fc7\u4e0emiR-770\u20135p\u76f8\u4e92\u4f5c\u7528\u6765\u8c03\u63a7TRIM29\u7684\u8868\u8fbe\u3002\u540e\u7eed\u7684\u5b9e\u9a8c\u9a8c\u8bc1\u4e86circRPS29\u4e0emiR-770\u20135p\u4ee5\u53caTRIM29 mRNA 3’UTR\u7684\u7ed3\u5408\u4f4d\u70b9\uff0c\u5e76\u901a\u8fc7\u8367\u5149\u7d20\u9176\u62a5\u544a\u57fa\u56e0\u5206\u6790\u3001RNA\u514d\u75ab\u6c89\u6dc0\u548ccircRNA pull-down\u5b9e\u9a8c\u8fdb\u4e00\u6b65\u8bc1\u5b9e\u4e86\u8fd9\u4e00\u8c03\u63a7\u8f74\u7684\u5b58\u5728\uff08\u56fe4D-G\uff09\u3002\u8fd9\u4e9b\u7ed3\u679c\u63ed\u793a\u4e86\u4e00\u4e2a<\/span>\u65b0\u7684\u8c03\u63a7\u8f74\uff0c\u53ef\u80fd\u5728PDAC\u7684GEM\u62b5\u6297\u6027\u4e2d\u8d77\u7740\u91cd\u8981\u4f5c\u7528<\/span>\u3002<\/span><\/span><\/p>\n

\"\u201c\u5e72\u6e7f\u7ed3\u5408\u201d\u53d124\u5206\u6587\u7ae0\uff0c\u6027\u4ef7\u6bd4MAX\uff01\u5357\u65b9\u533b\u79d1\u5927\u5b66\u8303\u5e94\u65b9\u7b49\u56e2\u961f\u6559\u4f60\u751f\u4fe1\u4e0e\u5b9e\u9a8c\u65e0\u7f1d\u5bf9\u63a5\uff0c\u5c55\u73b0\u79d1\u7814\u65e0\u9650\u53ef\u80fd\uff01\"<\/span> \u00a0 \u00a0<\/span><\/p>\n

5. circRPS29\/miR-770\u20135p\/TRIM29-MEK\/ERK\u4fe1\u53f7\u8f74\u7684\u673a\u5236\u9a8c\u8bc1<\/span><\/p>\n

\u8367\u5149\u7d20\u9176\u62a5\u544a\u57fa\u56e0<\/span>\u5206\u6790\u8bc1\u5b9e\u4e86circRPS29\u548cmiR-770\u20135p\u4ee5\u53camiR-770\u20135p\u548cTRIM29 mRNA 3’UTR\u4e4b\u95f4\u7684\u76f4\u63a5\u76f8\u4e92\u4f5c\u7528\uff08\u56fe5A-D\uff09\u3002<\/span>\u8367\u5149\u539f\u4f4d\u6742\u4ea4\uff08FISH\uff09<\/span>\u548c<\/span>RNA\u514d\u75ab\u6c89\u6dc0\uff08RIP\uff09<\/span>\u5b9e\u9a8c\u663e\u793a\u4e86circRPS29\u548cmiR-770\u20135p\u5728\u7ec6\u80de\u8d28\u4e2d\u7684\u5171\u5b9a\u4f4d\uff0c\u4ee5\u53ca\u5b83\u4eec\u4e0eAGO2\u86cb\u767d\u5f62\u6210\u7684\u590d\u5408\u4f53\uff08\u56fe5E\u548cF\uff09\u3002\u6b64\u5916\uff0c<\/span>RNA pull-down<\/span>\u5b9e\u9a8c\u8fdb\u4e00\u6b65\u8bc1\u5b9e\u4e86circRPS29\u548cmiR-770\u20135p\u4e4b\u95f4\u7684\u76f4\u63a5\u76f8\u4e92\u4f5c\u7528\uff08\u56fe5G\uff09\u3002<\/span>\u86cb\u767d\u514d\u75ab\u5370\u8ff9\u5206\u6790<\/span>\u63ed\u793a\u4e86circRPS29\u6572\u4f4e\u6216miR-770\u20135p\u8fc7\u8868\u8fbe\u6291\u5236\u4e86TRIM29\u3001pMEK\u548cpERK\u7684\u8868\u8fbe\uff0c\u800cmiR-770\u20135p\u7684\u6291\u5236\u5219\u9006\u8f6c\u4e86\u8fd9\u4e9b\u6548\u5e94\uff08\u56fe5H\u548cI\uff09\u3002\u8fd9\u4e9b\u7ed3\u679c\u5171\u540c\u8bc1\u660e\u4e86<\/span>circRPS29\u901a\u8fc7miR-770\u20135p\u8c03\u63a7TRIM29\u7684\u8868\u8fbe\uff0c\u5e76\u8fdb\u800c\u6fc0\u6d3bMEK\/ERK\u4fe1\u53f7\u901a\u8def\uff0c\u4fc3\u8fdbPDAC\u7684GEM\u62b5\u6297\u6027<\/span>\u3002<\/span><\/span><\/p>\n

\"\u201c\u5e72\u6e7f\u7ed3\u5408\u201d\u53d124\u5206\u6587\u7ae0\uff0c\u6027\u4ef7\u6bd4MAX\uff01\u5357\u65b9\u533b\u79d1\u5927\u5b66\u8303\u5e94\u65b9\u7b49\u56e2\u961f\u6559\u4f60\u751f\u4fe1\u4e0e\u5b9e\u9a8c\u65e0\u7f1d\u5bf9\u63a5\uff0c\u5c55\u73b0\u79d1\u7814\u65e0\u9650\u53ef\u80fd\uff01\"<\/span><\/p>\n

6. TRIM29\u633d\u6551circRPS29\u6572\u4f4e\u5bf9PDAC GEM\u62b5\u6297\u6027\u7684\u6291\u5236\u6548\u5e94<\/span><\/p>\n

\u514b\u9686\u5f62\u6210\u5b9e\u9a8c\u548cEdU\u63ba\u5165\u5b9e\u9a8c<\/span>\u663e\u793a\uff0cTRIM29\u7684\u8fc7\u8868\u8fbe\u53ef\u4ee5\u9006\u8f6ccircRPS29\u6572\u4f4e\u5f15\u8d77\u7684\u7ec6\u80de\u589e\u6b96\u548cDNA\u5408\u6210\u7684\u6291\u5236\uff08\u56fe6A\u548cB\uff09\u3002<\/span>\u7ec6\u80de\u5468\u671f\u5206\u6790<\/span>\u8868\u660e\uff0cTRIM29\u7684\u8fc7\u8868\u8fbe\u80fd\u591f\u9006\u8f6ccircRPS29\u6572\u4f4e\u8bf1\u5bfc\u7684\u7ec6\u80de\u5468\u671f\u505c\u6ede\uff08\u56fe6C\uff09\u3002<\/span>Transwell\u4fb5\u88ad\u5b9e\u9a8c<\/span>\u8fdb\u4e00\u6b65\u8bc1\u5b9e\u4e86TRIM29\u8fc7\u8868\u8fbe\u80fd\u591f\u589e\u5f3aPDAC\u8010\u836f\u7ec6\u80de\u7684\u4fb5\u88ad\u80fd\u529b\uff0c\u800c\u8fd9\u79cd\u80fd\u529b\u88abcircRPS29\u7684\u6572\u4f4e\u6240\u6291\u5236\uff08\u56fe6D\uff09\u3002<\/span>Western Blot\u5206\u6790<\/span>\u63ed\u793a\u4e86TRIM29\u8fc7\u8868\u8fbe\u80fd\u591f\u9006\u8f6ccircRPS29\u6572\u4f4e\u5f15\u8d77\u7684TRIM29\u3001pMEK\u548cpERK\u8868\u8fbe\u7684\u964d\u4f4e\uff08\u56fe6E\uff09\u3002\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\uff0c<\/span>TRIM29\u662fcircRPS29\u4e0b\u6e38\u7684\u6548\u5e94\u5206\u5b50\uff0c\u5e76\u4e14circRPS29\u901a\u8fc7TRIM29\u4fc3\u8fdb\u4e86PDAC\u7684GEM\u62b5\u6297\u6027\u3002<\/span><\/span><\/p>\n

\"\u201c\u5e72\u6e7f\u7ed3\u5408\u201d\u53d124\u5206\u6587\u7ae0\uff0c\u6027\u4ef7\u6bd4MAX\uff01\u5357\u65b9\u533b\u79d1\u5927\u5b66\u8303\u5e94\u65b9\u7b49\u56e2\u961f\u6559\u4f60\u751f\u4fe1\u4e0e\u5b9e\u9a8c\u65e0\u7f1d\u5bf9\u63a5\uff0c\u5c55\u73b0\u79d1\u7814\u65e0\u9650\u53ef\u80fd\uff01\"<\/span><\/p>\n

\u6587\u7ae0\u5c0f\u7ed3<\/span><\/p>\n

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