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rel=\"noopener\">#\u7ed8\u56fe<\/a><\/p>\n<\/div>\n<p style=\"text-align: center;\" data-mpa-powered-by=\"yiban.io\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.18518518518518517\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_YHrH5kBzN1unDT7eHqLtdZO1vwdtrw-8.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/zcicibWZessAxZibD6iaowp2Zv5dgyZQqBVAac9npnibJvGYo1ZFiafpiayXgbaYHrH5kBzN1unDT7eHqLtdZO1vwdtrw\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<section style=\"box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<p style=\"white-space: normal;box-sizing: border-box;\" powered-by=\"xiumi.us\"><span style=\"color: rgb(255, 129, 36);box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">\u5bcc\u96c6\u5206\u6790<\/strong><\/span>\u662f\u6211\u4eec\u79d1\u7814\u5206\u6790\u4e2d\u5e38\u89c1\u7684\u4e00\u79cd\u5206\u6790\u65b9\u5f0f\uff0c\u4eba\u7684\u5206\u6790\u8fc7\u7a0b\u5df2\u7ecf\u5f88\u6e05\u695a\u4e86\uff0c\u800c\u4e14\u6709\u5728\u7ebf\u5de5\u5177\u53ef\u4ee5\u610f\u89c1\u5bcc\u96c6\uff0c\u4f46\u4e00\u4e9b\u975e\u4eba\u7269\u79cd\uff0c\u8fd8\u9700\u8981\u501f\u52a9\u4e00\u4e9b\u4ee3\u7801\u6765\u5b8c\u6210\uff0c\u5bcc\u96c6\u5206\u6790\u4e2d\u8f83\u5e38\u89c1\u7684\u5c31\u662f<span style=\"color: rgb(255, 129, 36);box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">clusterProfiler<\/strong><\/span>\uff0c\u4e0b\u9762\u5c31\u8ba9\u6211\u4eec\u4ee5\u5c0f\u9f20\u4e3a\u4f8b\uff0c\u6765\u770b\u770b\u5bcc\u96c6\u5206\u6790\u662f\u600e\u4e48\u505a\u7684\u5427\u3002<br style=\"box-sizing: border-box;\" \/><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\" powered-by=\"xiumi.us\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;max-width: 100%;height: auto;line-height: 0;padding-right: 5px;box-sizing: border-box;\">\n<section style=\"transform: perspective(0px);-webkit-transform: perspective(0px);-moz-transform: perspective(0px);-o-transform: perspective(0px);transform-style: flat;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;transform: rotateY(180deg);line-height: 0;box-sizing: border-box;\">\n<section style=\"max-width: 100%;vertical-align: middle;display: inline-block;line-height: 0;width: 21px;height: auto;box-sizing: border-box;\"><img decoding=\"async\" data-ratio=\"0.8823529\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2022-12-02_\u6a21\u5f0f\u751f\u7269\u4e5f\u80fd\u770b\u529f\u80fd\uff0c\u975e\u4eba\u7269\u79cd\u7684\u5bcc\u96c6\u5206\u6790\/2_7tj7DXbKYkjAr9InoVhjQQT5JcHhtg.svg\" onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2022-12-02_\u6a21\u5f0f\u751f\u7269\u4e5f\u80fd\u770b\u529f\u80fd\uff0c\u975e\u4eba\u7269\u79cd\u7684\u5bcc\u96c6\u5206\u6790\/5_7tj7DXbKYkjAr9InoVhjQQT5JcHhtg.svg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_svg\/oYwP0cFmRU2fibTmhzuxe0IiaDoxQNDRQibMjVKxoy1OmDibrB1jQu9Ru44oUzVajx5OK47tj7DXbKYkjAr9InoVhjQQT5JcHhtg\/640?wx_fmt=svg''\" data-type=\"svg\" data-w=\"170\" style=\"vertical-align: middle;width: 100%;height: 100%;box-sizing: border-box;\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;max-width: 100%;height: auto;padding: 3px 20px;background-color: rgb(255, 128, 52);box-sizing: border-box;\">\n<section style=\"text-align: justify;color: rgb(255, 255, 255);box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">\u4ee3\u7801\u5982\u4e0b<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\">library(clusterProfiler) <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#\u52a0\u8f7d\u7a0b\u5e8f\u5305<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">library(org.Mm.eg.db)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">dif_enrich<- read.table(<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"T2DMvsCon.txt\"<\/span>,header = <span class=\"code-snippet__built_in\" style=\"box-sizing: border-box;\">T<\/span>,row.names = <span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">1<\/span>,sep = <span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"t\"<\/span>)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"><span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#\u8bfb\u53d6\u8868\u8fbe\u6587\u4ef6<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\">gene <- rownames(dif_enrich)<span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\"># \u8f6c\u4e3a\u5411\u91cf<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"><span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">## \u5c06gene symbol\u8f6c\u6362\u4e3aEntrez ID<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> gene.id <- bitr(gene, fromType=<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"SYMBOL\"<\/span>, toType=<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"ENTREZID\"<\/span>, OrgDb=<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"org.Mm.eg.db\"<\/span>)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"><span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#org.Mm.eg.db\u5305\u91cc\u8bb0\u8f7d\u4e86\u5c0f\u9f20\u57fa\u56e0ID\u7684\u5bf9\u5e94\u5173\u7cfb<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> egoBP<-enrichGO(gene = gene.id$ENTREZID, pAdjustMethod =<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"none\"<\/span>, <\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> OrgDb = <span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">'org.Mm.eg.db'<\/span>, <\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"> ont = <span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"BP\"<\/span>, <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#GO\u5bcc\u96c6\u6709\u591a\u79cd\u5206\u7c7b\uff0c\u5176\u4e2d\u6700\u91cd\u8981\u7684\u5c31\u662fBP\uff0c\u4e5f\u5c31\u662f\u751f\u7269\u5b66\u8fc7\u7a0b\uff0c\u8fd8\u53ef\u4ee5\u9009\u62e9MF\u5206\u5b50\u529f\u80fd\u548cCC\u7ec6\u80de\u7ed3\u6784\u3002\u5982\u679c\u5168\u90fd\u8981\u7684\u8bdd\u53ef\u4ee5\u9009\u62e9ALL<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"> pvalueCutoff = <span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">0.05<\/span>, <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#P\u5c0f\u4e8e0.05\uff0c\u5373\u4e3a\u663e\u8457\u5bcc\u96c6\uff0c\u8fd9\u91cc\u7684\u8bbe\u7f6e\u5c31\u662f\u53ea\u4fdd\u7559\u663e\u8457\u5bcc\u96c6\u7684\u7ed3\u679c\uff0c\u5982\u679c\u60f3\u628a\u4e0d\u663e\u8457\u7684\u4e5f\u90fd\u4fdd\u5b58\u4e0b\u6765\uff0c\u8fd9\u91cc\u53ef\u4ee5\u9009\u62e9\u628a0.05\u6539\u62101<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> qvalueCutoff=<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">1<\/span>,<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> minGSSize = <span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">10<\/span>,<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> readable= <span class=\"code-snippet__literal\" style=\"box-sizing: border-box;\">TRUE<\/span>) <\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"> summary(egoBP) <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\"># \u7ed3\u679c\u5c55\u793a<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"> bp2 <- simplify(egoBP, cutoff=<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">0.7<\/span>, by=<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"p.adjust\"<\/span>, select_fun=<span class=\"code-snippet__built_in\" style=\"box-sizing: border-box;\">min<\/span>) <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#\u5bf9\u5bcc\u96c6\u5230\u7684GO\u9879\u8fdb\u884c\u7b80\u5316\u5904\u7406\u3002<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"> write.table(summary(bp2), <span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"GO.BP.txt\"<\/span>, sep=<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"t\"<\/span>) <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#\u5c06\u5bcc\u96c6\u7ed3\u679c\u5199\u5165\u6587\u4ef6<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> pdf(<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"GO.bp.pdf\"<\/span>,height=<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">8<\/span>,width=<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">8<\/span>)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"> barplot(bp2, showCategory=<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">8<\/span>) <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#\u6761\u5f62\u56fe showCategory\u8fd9\u4e2a\u53c2\u6570\u662f\u7528\u6765\u63a7\u5236\u663e\u793a\u7684GO\u9879\u7684\u6570\u91cf\u7684\uff0c\u9ed8\u8ba4\u662f10\uff0c\u5177\u4f53\u6570\u91cf\u53ef\u6839\u636e\u9700\u8981\u6765\u8c03\u6574<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> dev.off()<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> pdf(<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"GOdot.bp.pdf\"<\/span>,height=<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">8<\/span>,width=<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">8<\/span>)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"> dotplot(egoBP)<span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#\u6c14\u6ce1\u56fe<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> dev.off()<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"max-width: 100%;vertical-align: middle;display: inline-block;line-height: 0;box-sizing: border-box;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.2265758\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_NozKxcMNOQ3NVd5eW3tjMbxV45ic3A-2.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAx2iaXmKPcW6NYAelWiaT3HqZXdQncXrDRbAMqNKrzSQF4LuJNNozKxcMNOQ3NVd5eW3tjMbxV45ic3A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"587\" style=\"vertical-align: middle;width: 100%;box-sizing: border-box;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\">egoPathway<-enrichKEGG(gene = gene.id$ENTREZID, organism = <span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"mmu\"<\/span>, pvalueCutoff = <span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">0.05<\/span>,pAdjustMethod =<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"none\"<\/span>,minGSSize =<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">2<\/span>,qvalueCutoff = <span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">1<\/span>) <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#KEGG\u5bcc\u96c6\uff0c\u4e0eGO\u5bcc\u96c6\u7c7b\u4f3c\uff0cmmu\u4ee3\u8868\u5c0f\u9f20 <\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"> summary(egoPathway) <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\"># \u7ed3\u679c\u5c55\u793a<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> pdf(<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"pathway.pdf\"<\/span>)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> barplot(egoPathway, showCategory=<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">8<\/span>) <\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> dev.off()<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> pdf(<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"pathwaydot.pdf\"<\/span>)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> dotplot(egoPathway)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> dev.off()<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"max-width: 100%;vertical-align: middle;display: inline-block;line-height: 0;box-sizing: border-box;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.8448845\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_UZaPD7aicnbIp7IDneeBPdIrwC1oOQ-2.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAx2iaXmKPcW6NYAelWiaT3HqZiaEBiboTjAO7gwAYf9aljgJhkqPUZaPD7aicnbIp7IDneeBPdIrwC1oOQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"606\" style=\"vertical-align: middle;width: 100%;box-sizing: border-box;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;max-width: 100%;height: auto;box-sizing: border-box;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row nowrap;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(255, 129, 36);min-width: 5%;max-width: 100%;height: auto;padding-right: 4px;padding-left: 4px;box-sizing: border-box;\">\n<section style=\"text-align: justify;color: rgb(255, 255, 255);font-size: 12px;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">TIPS<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;max-width: 100%;flex: 0 0 auto;height: auto;align-self: center;box-sizing: border-box;\">\n<section style=\"transform: translate3d(7px, 0px, 0px);box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: justify;font-size: 15px;color: rgb(95, 156, 239);box-sizing: border-box;\">\n<p style=\"text-align: left;white-space: normal;box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">\u516c\u4f17\u53f7\u56de\u590d<span style=\"color: rgb(255, 129, 36);box-sizing: border-box;\">\u201c111\u201d<\/span>\u83b7\u53d6\u6587\u7ae0\u76f8\u5173\u6587\u4ef6<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;box-sizing: border-box;\" powered-by=\"xiumi.us\">\u597d\u4e86\uff0c\u8fd9\u5c31\u662f\u4eca\u5929\u7684\u4e3b\u8981\u5185\u5bb9\u4e86\uff0c\u4ee3\u7801\u8fd8\u662f\u6bd4\u8f83\u7b80\u5355\u7684\uff0c\u5c0f\u4f19\u4f34\u4eec\u6709\u4ec0\u4e48\u95ee\u9898\u7684\u8bdd\u6b22\u8fce\u6765\u548c\u5c0f\u4e91\u5206\u4eab\u8ba8\u8bba\u54df\u3002<br style=\"box-sizing: border-box;\" \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;max-width: 100%;height: auto;line-height: 0;padding-right: 5px;box-sizing: border-box;\">\n<section style=\"transform: perspective(0px);-webkit-transform: perspective(0px);-moz-transform: perspective(0px);-o-transform: perspective(0px);transform-style: flat;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;transform: rotateY(180deg);line-height: 0;box-sizing: border-box;\">\n<section style=\"max-width: 100%;vertical-align: middle;display: inline-block;line-height: 0;width: 21px;height: auto;box-sizing: border-box;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.8823529\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2022-12-02_\u6a21\u5f0f\u751f\u7269\u4e5f\u80fd\u770b\u529f\u80fd\uff0c\u975e\u4eba\u7269\u79cd\u7684\u5bcc\u96c6\u5206\u6790\/2_7tj7DXbKYkjAr9InoVhjQQT5JcHhtg.svg\" onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2022-12-02_\u6a21\u5f0f\u751f\u7269\u4e5f\u80fd\u770b\u529f\u80fd\uff0c\u975e\u4eba\u7269\u79cd\u7684\u5bcc\u96c6\u5206\u6790\/5_7tj7DXbKYkjAr9InoVhjQQT5JcHhtg.svg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_svg\/oYwP0cFmRU2fibTmhzuxe0IiaDoxQNDRQibMjVKxoy1OmDibrB1jQu9Ru44oUzVajx5OK47tj7DXbKYkjAr9InoVhjQQT5JcHhtg\/640?wx_fmt=svg''\" data-type=\"svg\" data-w=\"170\" style=\"vertical-align: middle;width: 100%;height: 100%;box-sizing: border-box;\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;max-width: 100%;height: auto;padding: 3px 20px;background-color: rgb(255, 128, 52);box-sizing: border-box;\">\n<section style=\"text-align: justify;color: rgb(255, 255, 255);box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">\u63a8\u8350\u9605\u8bfb<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<ul class=\"list-paddingleft-1\" style=\"list-style-type: square;\">\n<li style=\"font-size: 15px;\">\n<p style=\"white-space: normal;box-sizing: border-box;\" powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247483946&idx=1&sn=a78bc9b595f361befb069725e1570546&chksm=cf7c92eff80b1bf9ddc2a00d4a8289758cb963f969e32c0cb04c97ed17dba65f8c25c0b0f186&scene=21#wechat_redirect\" textvalue=\"\u5c0f\u679c\u6559\u4f60\u4e09\u5206\u949f\u770b\u61c2\u591a\u6761\u7ebf\u5171\u5b58\u7684ROC\u56fe\u7684R\u8bed\u8a00\u753b\u6cd5\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" style=\"text-decoration: underline;font-size: 15px;\" data-linktype=\"2\" rel=\"noopener\"><span style=\"font-size: 15px;\">\u5c0f\u679c\u6559\u4f60\u4e09\u5206\u949f\u770b\u61c2\u591a\u6761\u7ebf\u5171\u5b58\u7684ROC\u56fe\u7684R\u8bed\u8a00\u753b\u6cd5<\/span><\/a><br style=\"box-sizing: border-box;\" \/><\/p>\n<\/li>\n<li style=\"font-size: 15px;\">\n<p style=\"white-space: normal;box-sizing: border-box;\" powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247483939&idx=1&sn=a4a1409ea38d2cfeede09d0c068ba549&chksm=cf7c92e6f80b1bf05281bf235624c34638d3fc134b5e30c1dffbabe741aab95b0fe0adc7a6e6&scene=21#wechat_redirect\" textvalue=\"\u975e\u764c\u75c7\u4e5f\u80fd\u505a\u9010\u6b65\uff0clogistic\u9010\u6b65\u56de\u5f52\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" style=\"text-decoration: underline;font-size: 15px;\" data-linktype=\"2\" rel=\"noopener\"><span style=\"font-size: 15px;\">\u975e\u764c\u75c7\u4e5f\u80fd\u505a\u9010\u6b65\uff0clogistic\u9010\u6b65\u56de\u5f52<\/span><\/a><br \/><\/p>\n<\/li>\n<li style=\"font-size: 15px;\">\n<p style=\"white-space: normal;box-sizing: border-box;\" powered-by=\"xiumi.us\"><a target=\"_blank\" 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data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"\u6a21\u5f0f\u751f\u7269\u4e5f\u80fd\u770b\u529f\u80fd\uff0c\u975e\u4eba\u7269\u79cd\u7684\u5bcc\u96c6\u5206\u6790\",\"time\":\"2022-12-02 19:00:50\",\"timeStamp\":\"1669978850\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247483975&idx=1&sn=6fe698647fb03fbf77e55ec08802cea9&chksm=cf7c9282f80b1b94c8efa6fba4b80c26de3cdc2762e53a3eeb6fd341ffd4b547ef78f0d59b6c#rd\" target=\"_blank\" rel=\"noopener\">\u6a21\u5f0f\u751f\u7269\u4e5f\u80fd\u770b\u529f\u80fd\uff0c\u975e\u4eba\u7269\u79cd\u7684\u5bcc\u96c6\u5206\u6790<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span id=\"copyright_logo\" class=\"wx_tap_link js_wx_tap_highlight rich_media_meta icon_appmsg_tag appmsg_title_tag weui-wa-hotarea\">\u539f\u521b <\/span><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u679c 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rel=\"noopener\">#\u7ed8\u56fe<\/a><\/p>\n<\/div>\n<p style=\"text-align: center;\" data-mpa-powered-by=\"yiban.io\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.18518518518518517\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_YHrH5kBzN1unDT7eHqLtdZO1vwdtrw-8.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/zcicibWZessAxZibD6iaowp2Zv5dgyZQqBVAac9npnibJvGYo1ZFiafpiayXgbaYHrH5kBzN1unDT7eHqLtdZO1vwdtrw\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<section style=\"box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<p style=\"white-space: normal;box-sizing: border-box;\" powered-by=\"xiumi.us\"><span style=\"color: rgb(255, 129, 36);box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">\u5bcc\u96c6\u5206\u6790<\/strong><\/span>\u662f\u6211\u4eec\u79d1\u7814\u5206\u6790\u4e2d\u5e38\u89c1\u7684\u4e00\u79cd\u5206\u6790\u65b9\u5f0f\uff0c\u4eba\u7684\u5206\u6790\u8fc7\u7a0b\u5df2\u7ecf\u5f88\u6e05\u695a\u4e86\uff0c\u800c\u4e14\u6709\u5728\u7ebf\u5de5\u5177\u53ef\u4ee5\u610f\u89c1\u5bcc\u96c6\uff0c\u4f46\u4e00\u4e9b\u975e\u4eba\u7269\u79cd\uff0c\u8fd8\u9700\u8981\u501f\u52a9\u4e00\u4e9b\u4ee3\u7801\u6765\u5b8c\u6210\uff0c\u5bcc\u96c6\u5206\u6790\u4e2d\u8f83\u5e38\u89c1\u7684\u5c31\u662f<span style=\"color: rgb(255, 129, 36);box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">clusterProfiler<\/strong><\/span>\uff0c\u4e0b\u9762\u5c31\u8ba9\u6211\u4eec\u4ee5\u5c0f\u9f20\u4e3a\u4f8b\uff0c\u6765\u770b\u770b\u5bcc\u96c6\u5206\u6790\u662f\u600e\u4e48\u505a\u7684\u5427\u3002<br style=\"box-sizing: border-box;\" \/><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\" powered-by=\"xiumi.us\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;max-width: 100%;height: auto;line-height: 0;padding-right: 5px;box-sizing: border-box;\">\n<section style=\"transform: perspective(0px);-webkit-transform: perspective(0px);-moz-transform: perspective(0px);-o-transform: perspective(0px);transform-style: flat;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;transform: rotateY(180deg);line-height: 0;box-sizing: border-box;\">\n<section style=\"max-width: 100%;vertical-align: middle;display: inline-block;line-height: 0;width: 21px;height: auto;box-sizing: border-box;\"><img decoding=\"async\" data-ratio=\"0.8823529\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2022-12-02_\u6a21\u5f0f\u751f\u7269\u4e5f\u80fd\u770b\u529f\u80fd\uff0c\u975e\u4eba\u7269\u79cd\u7684\u5bcc\u96c6\u5206\u6790\/2_7tj7DXbKYkjAr9InoVhjQQT5JcHhtg.svg\" onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2022-12-02_\u6a21\u5f0f\u751f\u7269\u4e5f\u80fd\u770b\u529f\u80fd\uff0c\u975e\u4eba\u7269\u79cd\u7684\u5bcc\u96c6\u5206\u6790\/5_7tj7DXbKYkjAr9InoVhjQQT5JcHhtg.svg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_svg\/oYwP0cFmRU2fibTmhzuxe0IiaDoxQNDRQibMjVKxoy1OmDibrB1jQu9Ru44oUzVajx5OK47tj7DXbKYkjAr9InoVhjQQT5JcHhtg\/640?wx_fmt=svg''\" data-type=\"svg\" data-w=\"170\" style=\"vertical-align: middle;width: 100%;height: 100%;box-sizing: border-box;\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;max-width: 100%;height: auto;padding: 3px 20px;background-color: rgb(255, 128, 52);box-sizing: border-box;\">\n<section style=\"text-align: justify;color: rgb(255, 255, 255);box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">\u4ee3\u7801\u5982\u4e0b<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\">library(clusterProfiler) <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#\u52a0\u8f7d\u7a0b\u5e8f\u5305<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">library(org.Mm.eg.db)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">dif_enrich<- read.table(<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"T2DMvsCon.txt\"<\/span>,header = <span class=\"code-snippet__built_in\" style=\"box-sizing: border-box;\">T<\/span>,row.names = <span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">1<\/span>,sep = <span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"t\"<\/span>)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"><span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#\u8bfb\u53d6\u8868\u8fbe\u6587\u4ef6<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\">gene <- rownames(dif_enrich)<span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\"># \u8f6c\u4e3a\u5411\u91cf<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"><span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">## \u5c06gene symbol\u8f6c\u6362\u4e3aEntrez ID<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> gene.id <- bitr(gene, fromType=<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"SYMBOL\"<\/span>, toType=<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"ENTREZID\"<\/span>, OrgDb=<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"org.Mm.eg.db\"<\/span>)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"><span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#org.Mm.eg.db\u5305\u91cc\u8bb0\u8f7d\u4e86\u5c0f\u9f20\u57fa\u56e0ID\u7684\u5bf9\u5e94\u5173\u7cfb<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> egoBP<-enrichGO(gene = gene.id$ENTREZID, pAdjustMethod =<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"none\"<\/span>, <\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> OrgDb = <span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">'org.Mm.eg.db'<\/span>, <\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"> ont = <span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"BP\"<\/span>, <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#GO\u5bcc\u96c6\u6709\u591a\u79cd\u5206\u7c7b\uff0c\u5176\u4e2d\u6700\u91cd\u8981\u7684\u5c31\u662fBP\uff0c\u4e5f\u5c31\u662f\u751f\u7269\u5b66\u8fc7\u7a0b\uff0c\u8fd8\u53ef\u4ee5\u9009\u62e9MF\u5206\u5b50\u529f\u80fd\u548cCC\u7ec6\u80de\u7ed3\u6784\u3002\u5982\u679c\u5168\u90fd\u8981\u7684\u8bdd\u53ef\u4ee5\u9009\u62e9ALL<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"> pvalueCutoff = <span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">0.05<\/span>, <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#P\u5c0f\u4e8e0.05\uff0c\u5373\u4e3a\u663e\u8457\u5bcc\u96c6\uff0c\u8fd9\u91cc\u7684\u8bbe\u7f6e\u5c31\u662f\u53ea\u4fdd\u7559\u663e\u8457\u5bcc\u96c6\u7684\u7ed3\u679c\uff0c\u5982\u679c\u60f3\u628a\u4e0d\u663e\u8457\u7684\u4e5f\u90fd\u4fdd\u5b58\u4e0b\u6765\uff0c\u8fd9\u91cc\u53ef\u4ee5\u9009\u62e9\u628a0.05\u6539\u62101<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> qvalueCutoff=<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">1<\/span>,<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> minGSSize = <span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">10<\/span>,<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> readable= <span class=\"code-snippet__literal\" style=\"box-sizing: border-box;\">TRUE<\/span>) <\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"> summary(egoBP) <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\"># \u7ed3\u679c\u5c55\u793a<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"> bp2 <- simplify(egoBP, cutoff=<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">0.7<\/span>, by=<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"p.adjust\"<\/span>, select_fun=<span class=\"code-snippet__built_in\" style=\"box-sizing: border-box;\">min<\/span>) <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#\u5bf9\u5bcc\u96c6\u5230\u7684GO\u9879\u8fdb\u884c\u7b80\u5316\u5904\u7406\u3002<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"> write.table(summary(bp2), <span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"GO.BP.txt\"<\/span>, sep=<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"t\"<\/span>) <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#\u5c06\u5bcc\u96c6\u7ed3\u679c\u5199\u5165\u6587\u4ef6<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> pdf(<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"GO.bp.pdf\"<\/span>,height=<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">8<\/span>,width=<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">8<\/span>)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"> barplot(bp2, showCategory=<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">8<\/span>) <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#\u6761\u5f62\u56fe showCategory\u8fd9\u4e2a\u53c2\u6570\u662f\u7528\u6765\u63a7\u5236\u663e\u793a\u7684GO\u9879\u7684\u6570\u91cf\u7684\uff0c\u9ed8\u8ba4\u662f10\uff0c\u5177\u4f53\u6570\u91cf\u53ef\u6839\u636e\u9700\u8981\u6765\u8c03\u6574<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> dev.off()<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> pdf(<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"GOdot.bp.pdf\"<\/span>,height=<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">8<\/span>,width=<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">8<\/span>)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"> dotplot(egoBP)<span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#\u6c14\u6ce1\u56fe<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> dev.off()<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"max-width: 100%;vertical-align: middle;display: inline-block;line-height: 0;box-sizing: border-box;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.2265758\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_NozKxcMNOQ3NVd5eW3tjMbxV45ic3A-2.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAx2iaXmKPcW6NYAelWiaT3HqZXdQncXrDRbAMqNKrzSQF4LuJNNozKxcMNOQ3NVd5eW3tjMbxV45ic3A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"587\" style=\"vertical-align: middle;width: 100%;box-sizing: border-box;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\">egoPathway<-enrichKEGG(gene = gene.id$ENTREZID, organism = <span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"mmu\"<\/span>, pvalueCutoff = <span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">0.05<\/span>,pAdjustMethod =<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"none\"<\/span>,minGSSize =<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">2<\/span>,qvalueCutoff = <span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">1<\/span>) <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\">#KEGG\u5bcc\u96c6\uff0c\u4e0eGO\u5bcc\u96c6\u7c7b\u4f3c\uff0cmmu\u4ee3\u8868\u5c0f\u9f20 <\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\" style=\"box-sizing: border-box;\"> summary(egoPathway) <span class=\"code-snippet__comment\" style=\"box-sizing: border-box;\"># \u7ed3\u679c\u5c55\u793a<\/span><\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> pdf(<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"pathway.pdf\"<\/span>)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> barplot(egoPathway, showCategory=<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">8<\/span>) <\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> dev.off()<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> pdf(<span class=\"code-snippet__string\" style=\"box-sizing: border-box;\">\"pathwaydot.pdf\"<\/span>)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> dotplot(egoPathway)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\"> dev.off()<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"max-width: 100%;vertical-align: middle;display: inline-block;line-height: 0;box-sizing: border-box;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.8448845\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_UZaPD7aicnbIp7IDneeBPdIrwC1oOQ-2.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAx2iaXmKPcW6NYAelWiaT3HqZiaEBiboTjAO7gwAYf9aljgJhkqPUZaPD7aicnbIp7IDneeBPdIrwC1oOQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"606\" style=\"vertical-align: middle;width: 100%;box-sizing: border-box;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;max-width: 100%;height: auto;box-sizing: border-box;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row nowrap;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(255, 129, 36);min-width: 5%;max-width: 100%;height: auto;padding-right: 4px;padding-left: 4px;box-sizing: border-box;\">\n<section style=\"text-align: justify;color: rgb(255, 255, 255);font-size: 12px;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">TIPS<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;max-width: 100%;flex: 0 0 auto;height: auto;align-self: center;box-sizing: border-box;\">\n<section style=\"transform: translate3d(7px, 0px, 0px);box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: justify;font-size: 15px;color: rgb(95, 156, 239);box-sizing: border-box;\">\n<p style=\"text-align: left;white-space: normal;box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">\u516c\u4f17\u53f7\u56de\u590d<span style=\"color: rgb(255, 129, 36);box-sizing: border-box;\">\u201c111\u201d<\/span>\u83b7\u53d6\u6587\u7ae0\u76f8\u5173\u6587\u4ef6<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;box-sizing: border-box;\" powered-by=\"xiumi.us\">\u597d\u4e86\uff0c\u8fd9\u5c31\u662f\u4eca\u5929\u7684\u4e3b\u8981\u5185\u5bb9\u4e86\uff0c\u4ee3\u7801\u8fd8\u662f\u6bd4\u8f83\u7b80\u5355\u7684\uff0c\u5c0f\u4f19\u4f34\u4eec\u6709\u4ec0\u4e48\u95ee\u9898\u7684\u8bdd\u6b22\u8fce\u6765\u548c\u5c0f\u4e91\u5206\u4eab\u8ba8\u8bba\u54df\u3002<br style=\"box-sizing: border-box;\" \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;max-width: 100%;height: auto;line-height: 0;padding-right: 5px;box-sizing: border-box;\">\n<section style=\"transform: perspective(0px);-webkit-transform: perspective(0px);-moz-transform: perspective(0px);-o-transform: perspective(0px);transform-style: flat;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;transform: rotateY(180deg);line-height: 0;box-sizing: border-box;\">\n<section style=\"max-width: 100%;vertical-align: middle;display: inline-block;line-height: 0;width: 21px;height: auto;box-sizing: border-box;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.8823529\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2022-12-02_\u6a21\u5f0f\u751f\u7269\u4e5f\u80fd\u770b\u529f\u80fd\uff0c\u975e\u4eba\u7269\u79cd\u7684\u5bcc\u96c6\u5206\u6790\/2_7tj7DXbKYkjAr9InoVhjQQT5JcHhtg.svg\" onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2022-12-02_\u6a21\u5f0f\u751f\u7269\u4e5f\u80fd\u770b\u529f\u80fd\uff0c\u975e\u4eba\u7269\u79cd\u7684\u5bcc\u96c6\u5206\u6790\/5_7tj7DXbKYkjAr9InoVhjQQT5JcHhtg.svg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_svg\/oYwP0cFmRU2fibTmhzuxe0IiaDoxQNDRQibMjVKxoy1OmDibrB1jQu9Ru44oUzVajx5OK47tj7DXbKYkjAr9InoVhjQQT5JcHhtg\/640?wx_fmt=svg''\" data-type=\"svg\" data-w=\"170\" style=\"vertical-align: middle;width: 100%;height: 100%;box-sizing: border-box;\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;max-width: 100%;height: auto;padding: 3px 20px;background-color: rgb(255, 128, 52);box-sizing: border-box;\">\n<section style=\"text-align: justify;color: rgb(255, 255, 255);box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">\u63a8\u8350\u9605\u8bfb<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<ul class=\"list-paddingleft-1\" style=\"list-style-type: square;\">\n<li style=\"font-size: 15px;\">\n<p style=\"white-space: normal;box-sizing: border-box;\" powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247483946&idx=1&sn=a78bc9b595f361befb069725e1570546&chksm=cf7c92eff80b1bf9ddc2a00d4a8289758cb963f969e32c0cb04c97ed17dba65f8c25c0b0f186&scene=21#wechat_redirect\" textvalue=\"\u5c0f\u679c\u6559\u4f60\u4e09\u5206\u949f\u770b\u61c2\u591a\u6761\u7ebf\u5171\u5b58\u7684ROC\u56fe\u7684R\u8bed\u8a00\u753b\u6cd5\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" style=\"text-decoration: underline;font-size: 15px;\" data-linktype=\"2\" rel=\"noopener\"><span style=\"font-size: 15px;\">\u5c0f\u679c\u6559\u4f60\u4e09\u5206\u949f\u770b\u61c2\u591a\u6761\u7ebf\u5171\u5b58\u7684ROC\u56fe\u7684R\u8bed\u8a00\u753b\u6cd5<\/span><\/a><br style=\"box-sizing: border-box;\" \/><\/p>\n<\/li>\n<li style=\"font-size: 15px;\">\n<p style=\"white-space: normal;box-sizing: border-box;\" powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247483939&idx=1&sn=a4a1409ea38d2cfeede09d0c068ba549&chksm=cf7c92e6f80b1bf05281bf235624c34638d3fc134b5e30c1dffbabe741aab95b0fe0adc7a6e6&scene=21#wechat_redirect\" textvalue=\"\u975e\u764c\u75c7\u4e5f\u80fd\u505a\u9010\u6b65\uff0clogistic\u9010\u6b65\u56de\u5f52\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" style=\"text-decoration: underline;font-size: 15px;\" data-linktype=\"2\" rel=\"noopener\"><span style=\"font-size: 15px;\">\u975e\u764c\u75c7\u4e5f\u80fd\u505a\u9010\u6b65\uff0clogistic\u9010\u6b65\u56de\u5f52<\/span><\/a><br \/><\/p>\n<\/li>\n<li style=\"font-size: 15px;\">\n<p style=\"white-space: normal;box-sizing: border-box;\" powered-by=\"xiumi.us\"><a target=\"_blank\" 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href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247483766&idx=1&sn=90b424cc1e9d0effb48e858801bf6e56&chksm=cf7c91b3f80b18a50ca28b8ac11ca41a4282a553736d9e93bdda02a2f04d43bbfc23707e3d3a&scene=21#wechat_redirect\" textvalue=\"\u975e\u764c\u75c7\u4e5f\u80fd\u770b\u98ce\u9669\uff0cR\u8bed\u8a00\u5355\u56e0\u7d20logistic\u56de\u5f52\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" style=\"text-decoration: underline;font-size: 15px;\" data-linktype=\"2\" rel=\"noopener\"><span style=\"font-size: 15px;\">\u975e\u764c\u75c7\u4e5f\u80fd\u770b\u98ce\u9669\uff0cR\u8bed\u8a00\u5355\u56e0\u7d20logistic\u56de\u5f52<\/span><\/a><br \/><\/p>\n<\/li>\n<\/ul>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: -5px;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: stretch;flex: 0 0 auto;background-color: rgb(226, 91, 91);min-width: 5%;max-width: 100%;height: auto;padding-top: 5px;padding-right: 9px;padding-left: 20px;box-sizing: border-box;\">\n<section style=\"margin-bottom: -5px;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: justify;color: rgb(255, 255, 255);box-sizing: border-box;\">\n<p style=\"white-space: normal;box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">\u626b\u7801\u5173\u6ce8\u6211\u4eec<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 5%;max-width: 100%;flex: 0 0 auto;height: auto;align-self: stretch;box-sizing: border-box;\">\n<section style=\"box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 38px 0px 0px 19px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(226, 91, 91);box-sizing: border-box;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 10px;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(226, 91, 91);height: 3px;box-sizing: border-box;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 20px;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: 37%;align-self: center;flex: 0 0 auto;height: auto;box-sizing: border-box;\">\n<section style=\"text-align: center;margin-top: 10px;line-height: 0;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"max-width: 100%;vertical-align: middle;display: inline-block;line-height: 0;width: 90%;height: auto;box-sizing: border-box;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_Ws7VoicntXP2lJRicyt0IGO8FLOjEA-2.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/zcicibWZessAx2iaXmKPcW6NYAelWiaT3HqZLOuicPlticafEdohC3x7Tz75ajvVWs7VoicntXP2lJRicyt0IGO8FLOjEA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"258\" style=\"vertical-align: middle;width: 100%;box-sizing: border-box;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 57%;align-self: center;flex: 0 0 auto;height: auto;box-sizing: border-box;\">\n<section style=\"text-align: right;margin-bottom: 4px;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(238, 216, 190);box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\">shengxinguoer<\/p>\n<\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: -4px;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: right;color: rgb(226, 91, 91);font-size: 23px;line-height: 1.1;box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">\u751f\u4fe1\u679c<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<\/section>\n<section style=\"text-align: center;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: right;box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\">\u751f\u4fe1\u786c\u6838\u77e5\u8bc6\u89e3\u7b54<\/p>\n<p style=\"box-sizing: 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[…]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/47889"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=47889"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/47889\/revisions"}],"predecessor-version":[{"id":47904,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/47889\/revisions\/47904"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=47889"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=47889"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=47889"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}