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onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/zcicibWZessAx2cF5xxW6pBGPmgTUt8qTlL9RiabvDGL72zZRhzS10IN01ZYt0CuCiczxXATRA1NT7QsFibQGILBxvQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"text-align: center;\"><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;font-size: 16px;white-space: normal;box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"box-sizing: border-box;\">\n<p powered-by=\"xiumi.us\" style=\"box-sizing: border-box;\">\u5f53\u6211\u4eec\u62ff\u5230\u4e00\u7ec4\u5355\u7ec6\u80de\u6570\u636e\u7684\u77e9\u9635\u65f6\uff0c\u5982\u4f55\u5728\u5904\u7406\u4e4b\u524d\u89c2\u5bdf\u7ec6\u80de\u548c\u57fa\u56e0\u8868\u8fbe\u4e4b\u95f4\u7684\u5173\u7cfb\u5462\uff1f\u4eca\u5929\u5c0f\u679c\u5c31\u5e26\u5927\u5bb6\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\uff01<\/p>\n<p powered-by=\"xiumi.us\" style=\"box-sizing: border-box;\"><br \/><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;box-sizing: border-box;\">\n<section style=\"padding-right: 12px;padding-left: 12px;display: inline-block;vertical-align: bottom;width: auto;background-color: rgb(147, 176, 108);min-width: 10%;max-width: 100%;flex: 0 0 auto;height: auto;align-self: flex-end;box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"font-size: 22px;color: rgb(255, 255, 255);text-align: justify;box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">1<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-left: 10px;display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;border-style: solid;border-width: 1px 0px;border-color: rgb(147, 176, 108) rgb(62, 62, 62);min-width: 10%;max-width: 100%;flex: 0 0 auto;height: auto;box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 4px;margin-bottom: 4px;box-sizing: border-box;\">\n<section style=\"color: rgb(147, 176, 108);text-align: justify;box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\">\u751f\u6210\u6570\u636e\u77e9\u9635<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\" style=\"box-sizing: border-box;\">\u4eca\u5929\u6211\u4eec\u7528\u5230\u7684\u6570\u636e\u4ecd\u7136\u6765\u81ea\u4e8escRNAseq\u5305\u4e2d\u7684\u7ec6\u80de\u96c6\u54e6\uff01\u6570\u636e\u77e9\u9635\u7684\u751f\u6210\u65b9\u6cd5\u5c0f\u679c\u5df2\u7ecf\u7ed9\u5927\u5bb6\u51c6\u5907\u597d\u5566\uff01\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\u5427\uff01<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">library(scRNAseq)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">data(fluidigm)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">fluidigm<-ReprocessedFluidigmData()<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">ct <- <span class=\"code-snippet__built_in\" style=\"box-sizing: border-box;\">floor<\/span>(assays(fluidigm)$rsem_counts) #\u751f\u6210\u6570\u636e\u77e9\u9635<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">ct[<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">1<\/span>:<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">4<\/span>,<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">1<\/span>:<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">4<\/span>]<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">sample_ann <- as.data.frame(colData(fluidigm))<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">DT::datatable(sample_ann)<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\"><br \/><\/p>\n<p style=\"box-sizing: border-box;\">\u4f7f\u7528R\u8bed\u8a00\u4e2d\u7684View()\u51fd\u6570\u53ef\u4ee5\u76f4\u63a5\u67e5\u770b\u6211\u4eec\u751f\u6210\u7684\u6570\u636e\u77e9\u9635\u54e6\uff0c\u6211\u4eec\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\u3002<\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.4649805447470817\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-05-06_scran\uff1a\u7ec6\u80de\u8868\u8fbe\u5927\u6570\u636e\u7684\u667a\u80fd\u5904\u7406\u5de5\u5177\uff0c\u52a9\u4f60\u6210\u4e3a\u8868\u8fbe\u5206\u6790\u4e13\u5bb6\uff01\/2_QXIaWm7icdSRAkbLhHhryBjuyOibNw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAwtJh5WPmw9Q70nJCdPAypjdTIuT71XzISibRWHfc7aNhnbuO7QXIaWm7icdSRAkbLhHhryBjuyOibNw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"514\"><\/p>\n<p style=\"box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;box-sizing: border-box;\">\n<section style=\"padding-right: 12px;padding-left: 12px;display: inline-block;vertical-align: bottom;width: auto;background-color: rgb(147, 176, 108);min-width: 10%;max-width: 100%;flex: 0 0 auto;height: auto;align-self: flex-end;box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"font-size: 22px;color: rgb(255, 255, 255);text-align: justify;box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\">2<br style=\"box-sizing: border-box;\" \/><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-left: 10px;display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;border-style: solid;border-width: 1px 0px;border-color: rgb(147, 176, 108) rgb(62, 62, 62);min-width: 10%;max-width: 100%;flex: 0 0 auto;height: auto;box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 4px;margin-bottom: 4px;box-sizing: border-box;\">\n<section style=\"color: rgb(147, 176, 108);text-align: justify;box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\">\u63a2\u7d22\u57fa\u56e0\u8868\u8fbe\u60c5\u51b5<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\">\u5728\u63a2\u7d22\u57fa\u56e0\u8868\u8fbe\u60c5\u51b5\uff0c\u5177\u4f53\u65f6\u4ece\u54ea\u4e9b\u89d2\u5ea6\u6765\u770b\u5462\uff1f\u5c0f\u679c\u5728\u8fd9\u91cc\u7ed9\u5927\u5bb6\u4f53\u5171\u4e24\u79cd\u601d\u8def\uff1a<\/p>\n<p style=\"box-sizing: border-box;\">1.\u7edf\u8ba1\u67d0\u4e2a\u57fa\u56e0\u5728\u591a\u5c11\u4e2a\u7ec6\u80de\u4e2d\u5f97\u5230\u8868\u8fbe\uff1b<\/p>\n<p style=\"box-sizing: border-box;\">2.\u7edf\u8ba1\u4e00\u4e2a\u7ec6\u80de\u4e2d\u8868\u8fbe\u4e86\u591a\u5c11\u4e2a\u57fa\u56e0\uff1b<\/p>\n<p style=\"box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<p style=\"box-sizing: border-box;\">\u90a3\u4e48\u8981\u4ece\u8fd9\u4e24\u4e2a\u89d2\u5ea6\u53bb\u63a2\u7d22\uff0c\u5206\u522b\u8981\u5982\u4f55\u53bb\u7edf\u8ba1\u5462\uff1f\u5feb\u8c03\u52a8\u4f60\u806a\u660e\u7684\u5c0f\u8111\u888b\uff01\u5982\u679c\u60f3\u4e0d\u5230\u4e5f\u6ca1\u5173\u7cfb\uff0c\u4eca\u5929\u5c0f\u679c\u624b\u628a\u624b\u6559\u4f60\u5b8c\u6210\uff01<\/p>\n<p style=\"box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<p style=\"box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">o \u7edf\u8ba1\u6240\u6709\u57fa\u56e0\u8868\u8fbe\u6210\u529f\u7684\u7ec6\u80de\u4e2a\u6570<\/strong><\/p>\n<p style=\"box-sizing: border-box;\">\u5bf9\u4e8e\u57fa\u56e0\u8868\u8fbe\u6210\u529f\u7684\u7ec6\u80de\u4e2a\u6570\uff0c\u6211\u4eec\u9700\u8981\u7528apply\u51fd\u6570\u7edf\u8ba1\u6570\u636e\u77e9\u9635\u4e2d\u6bcf\u4e00\u884c\u5143\u7d20\u4e2d\u5bf9\u5e94\u503c\u5927\u4e8e0\u7684\u4e2a\u6570\uff0c\u63a5\u4e0b\u6765\u6211\u4eec\u8fdb\u884c\u7edf\u8ba1\u5e76\u7ed8\u5236\u7bb1\u7ebf\u56fe\u89c2\u5bdf\u6574\u4f53\u8868\u8fbe\u60c5\u51b5\u3002\u548c\u5c0f\u679c\u4e00\u8d77\u6765\u770b\u770b\u5427\uff01<\/p>\n<p style=\"box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<p style=\"box-sizing: border-box;\"><span style=\"color: rgb(136, 136, 136);\">fivenum(apply(counts,1,function(x) sum(x>0) ))#\u7edf\u8ba1\u6240\u6709\u57fa\u56e0\u8868\u8fbe\u6210\u529f\u7ec6\u80de\u4e2a\u6570\u4e2d\u7684\u4e94\u4e2a\u6307\u6807<br style=\"box-sizing: border-box;\" \/>boxplot(apply(counts,1,function(x) sum(x>0) ))#\u7ed8\u5236\u7bb1\u7ebf\u56fe<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.574757281553398\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-05-06_scran\uff1a\u7ec6\u80de\u8868\u8fbe\u5927\u6570\u636e\u7684\u667a\u80fd\u5904\u7406\u5de5\u5177\uff0c\u52a9\u4f60\u6210\u4e3a\u8868\u8fbe\u5206\u6790\u4e13\u5bb6\uff01\/3_XWnOIJzejwOI236LR2I0Cpic0TEXDw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAwtJh5WPmw9Q70nJCdPAypjdvefqJwbc5c9lrSKdIcdHpnZ2XWnOIJzejwOI236LR2I0Cpic0TEXDw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"515\"><\/p>\n<p style=\"box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<p style=\"box-sizing: border-box;\">\u5f97\u5230\u4ee5\u4e0a\u7684\u7bb1\u7ebf\u56fe\u540e\uff0c\u5c0f\u679c\u6765\u7ed9\u5927\u5bb6\u505a\u4e00\u4e2a\u7b80\u5355\u7684\u89e3\u8bfb\u5427\uff01\u7531\u7bb1\u7ebf\u56fe\u7684\u4e2d\u4f4d\u7ebf\u53ef\u4ee5\u770b\u51fa\uff0c\u57fa\u56e0\u8868\u8fbe\u6210\u529f\u7684\u7ec6\u80de\u6570\u7684\u4e2d\u4f4d\u6570\u503c\u5927\u6982\u57283\u4e2a\u5de6\u53f3\uff0c\u8868\u8fbe\u7ec6\u80de\u6570\u7684\u9608\u503c\u5927\u6982\u572835\u5de6\u53f3\uff0c\u5373\u8bf4\u660e\u5982\u679c\u57fa\u56e0\u572835\u4e2a\u7ec6\u80de\u5185\u4e0d\u8868\u8fbe\u5c31\u53ef\u4ee5\u76f4\u63a5\u8fc7\u6ee4\u6389\u54e6\u3002<\/p>\n<p style=\"box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<p style=\"box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">o \u7edf\u8ba1\u6240\u6709\u7ec6\u80de\u8868\u8fbe\u4e86\u7684\u57fa\u56e0\u4e2a\u6570<\/strong><\/p>\n<p style=\"box-sizing: border-box;\">\u6709\u4e86\u4e0a\u9762\u7684\u57fa\u7840\uff0c\u4f60\u662f\u5426\u5b66\u4f1a\u4e3e\u4e00\u53cd\u4e09\u4e86\u5462\uff1f\u5bf9\u4e8e\u7ec6\u80de\u8868\u8fbe\u4e86\u7684\u57fa\u56e0\u4e2a\u6570\uff0c\u6211\u4eec\u540c\u6837\u4f7f\u7528apply\u51fd\u6570\u7edf\u8ba1\u6570\u636e\u77e9\u9635\u4e2d\u7684\u6bcf\u4e00\u5217\u5143\u7d20\u4e2d\u5bf9\u5e94\u503c\u5927\u4e8e0\u7684\u4e2a\u6570\u5373\u53ef\uff0c\u63a5\u4e0b\u6765\u6211\u4eec\u7528\u76f4\u65b9\u5206\u5e03\u56fe\u6765\u548c\u5927\u5bb6\u5c55\u793a\u4e00\u4e0b\u6574\u4f53\u7684\u8868\u8fbe\u60c5\u51b5\u3002\u548c\u5c0f\u679c\u4e00\u8d77\u770b\u770b\u5427\uff01<\/p>\n<p style=\"box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<p style=\"box-sizing: border-box;\"><span style=\"color: rgb(136, 136, 136);\">fivenum(apply(counts,2,function(x) sum(x>0) ))#\u7edf\u8ba1\u6240\u6709\u7ec6\u80de\u8868\u8fbe\u4e86\u57fa\u56e0\u4e2a\u6570\u4e2d\u7684\u4e94\u4e2a\u6307\u6807<br style=\"box-sizing: border-box;\" \/>hist(apply(counts,2,function(x) sum(x>0) )) #\u7ed8\u5236\u76f4\u65b9\u5206\u5e03\u56fe<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.7113970588235294\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-05-06_scran\uff1a\u7ec6\u80de\u8868\u8fbe\u5927\u6570\u636e\u7684\u667a\u80fd\u5904\u7406\u5de5\u5177\uff0c\u52a9\u4f60\u6210\u4e3a\u8868\u8fbe\u5206\u6790\u4e13\u5bb6\uff01\/4_Diae8vA44hOJbXgAMtdc63AbqLlW0g.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAwtJh5WPmw9Q70nJCdPAypja1YdelzYblFCkvWviaWUhTTWTuDiae8vA44hOJbXgAMtdc63AbqLlW0g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"544\"><span style=\"text-align: justify;\"><\/span><\/p>\n<p style=\"box-sizing: border-box;\"> \u5728\u5206\u6790\u4e86\u7bb1\u7ebf\u56fe\u7684\u7edf\u8ba1\u7ed3\u679c\u540e\uff0c\u4f60\u80fd\u5426\u72ec\u7acb\u5206\u6790\u76f4\u65b9\u56fe\u7684\u7edf\u8ba1\u7ed3\u679c\u5462\uff1f\u6211\u4eec\u8fd8\u662f\u548c\u5c0f\u679c\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\uff01<\/p>\n<p style=\"box-sizing: border-box;\"> \u7531\u76f4\u65b9\u5206\u5e03\u56fe\u7684\u7edf\u8ba1\u7ed3\u679c\u53ef\u4ee5\u770b\u51fa\uff0c\u8868\u8fbe\u57fa\u56e0\u4e2a\u6570\u57283000~4000\u4e4b\u95f4\u7684\u7ec6\u80de\u5360\u6700\u591a\u6570\u91cf\uff0c\u8868\u8fbe\u57fa\u56e0\u4e2a\u6570\u57288000\u4ee5\u4e0a\u7684\u7ec6\u80de\u4ec5\u67092~3\u4e2a\uff0c\u5360\u6700\u5c11\u7684\u6570\u91cf\u3002<\/p>\n<p style=\"box-sizing: border-box;\"> \u901a\u8fc7\u4ee5\u4e0a\u4e24\u7ec4\u7edf\u8ba1\u56fe\u7684\u5206\u6790\uff0c\u6211\u4eec\u5c31\u5bf9\u57fa\u56e0\u5728\u7ec6\u80de\u4e2d\u7684\u8868\u8fbe\u60c5\u51b5\u6e38\u5ba2\u6574\u4f53\u7684\u628a\u63e1\uff0c\u600e\u4e48\u6837\uff0c\u4eca\u5929\u7684\u5c0f\u6280\u5de7\u4f60\u5b66\u5e9f\u4e86\u561b\uff1f\u66f4\u591a\u751f\u4fe1\u6280\u5de7\u8bf7\u7ee7\u7eed\u5173\u6ce8\u5c0f\u679c\u54e6\uff01<\/p>\n<\/section>\n<p style=\"box-sizing: border-box;\"><br \/><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;box-sizing: border-box;\">\n<section style=\"display: inline-block;vertical-align: top;box-sizing: border-box;\">\n<section style=\"padding-right: 12px;padding-left: 12px;background-color: rgb(255, 255, 255);box-sizing: border-box;\">\n<p style=\"clear: none;box-sizing: border-box;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;font-size: 16px;white-space: normal;box-sizing: border-box;\">\n<section style=\"box-sizing: border-box;\">\n<section>\n<section style=\"box-sizing: border-box;max-width: 100%;\">\n<section>\n<section style=\"box-sizing: border-box;max-width: 100%;\">\n<section>\n<section style=\"box-sizing: border-box;max-width: 100%;\">\n<section>\n<section style=\"box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"box-sizing: border-box;\">\n<section style=\"line-height: 1.8;box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"box-sizing: border-box;\">\n<section style=\"box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 20px;display: flex;flex-flow: row nowrap;text-align: center;justify-content: center;box-sizing: border-box;\">\n<section style=\"display: inline-block;width: 543.108px;vertical-align: top;border-style: solid;border-width: 1px;border-color: rgb(67, 67, 67);flex: 0 0 auto;align-self: flex-start;height: auto;box-shadow: rgb(255, 219, 118) 6px 6px 0px;background-color: rgb(255, 255, 255);box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;box-sizing: border-box;\">\n<section style=\"display: inline-block;width: 378.611px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;box-sizing: border-box;\">\n<section style=\"text-align: center;box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\"><span style=\"font-size: 15px;box-sizing: border-box;\">\u751f\u4fe1\u4ebaR\u8bed\u8a00\u5b66\u4e60\u5fc5\u5907<\/span><\/strong><\/p>\n<p style=\"box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\"><span style=\"font-size: 15px;box-sizing: border-box;\">\u7acb\u523b\u62e5\u6709\u4e00\u4e2aRstudio\u8d26\u53f7<\/span><\/strong><\/p>\n<p style=\"box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\"><span style=\"font-size: 15px;box-sizing: border-box;\">\u5f00\u542f\u5347\u7ea7\u6a21\u5f0f\u5427<\/span><\/strong><\/p>\n<p style=\"box-sizing: border-box;\"><span style=\"color: rgb(255, 129, 36);box-sizing: border-box;font-size: 12px;\">\uff0856\u7ebf\u7a0b\uff0c256G\u5185\u5b58\uff0c\u4e2a\u4eba\u5b58\u50a81T\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;width: 162.257px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;line-height: 0;box-sizing: border-box;\">\n<section style=\"max-width: 100%;vertical-align: middle;display: inline-block;line-height: 0;box-sizing: border-box;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"162\" data-cropsely1=\"0\" data-cropsely2=\"162\" data-ratio=\"1\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-05-06_scran\uff1a\u7ec6\u80de\u8868\u8fbe\u5927\u6570\u636e\u7684\u667a\u80fd\u5904\u7406\u5de5\u5177\uff0c\u52a9\u4f60\u6210\u4e3a\u8868\u8fbe\u5206\u6790\u4e13\u5bb6\uff01\/5_ib2l1ye6HNIkwTG696C2pf9yAtdeGA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAyB69UyXDLG0wQcISNwmL6DlpIKVBnCSOXHTWJhLcxic2qtibaib2l1ye6HNIkwTG696C2pf9yAtdeGA\/640?wx_fmt=png'\" data-type=\"jpeg\" data-w=\"400\" style=\"height: 111px;vertical-align: middle;width: 111px;box-sizing: border-box;\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"box-sizing: border-box;\">\n<p style=\"text-align: center;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650750896&idx=2&sn=eedf639d72ed894b623b97cb453ec731&chksm=831a7820b46df136d60bbcd98da1545dc7cdc7d23013ee352ad58b8413bd8b74496a1cb24158&scene=21#wechat_redirect\" textvalue=\"\u4f60\u5df2\u9009\u4e2d\u4e86\u6dfb\u52a0\u94fe\u63a5\u7684\u5185\u5bb9\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><\/span><\/a><\/p>\n<section class=\"mp_profile_iframe_wrp\"><mp-common-profile class=\"js_uneditable custom_select_card mp_profile_iframe\" data-pluginname=\"mpprofile\" data-weui-theme=\"light\" data-id=\"Mzg3OTg3MjQyNg==\" data-headimg=\"http:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAwvyLLXlYq0SJu55voD1bnb7juHUdkAibE5woL3FrUvV4nmfhXjL5DgcNYqYFsyFCZrof6ibA2ibPmuw\/0?wx_fmt=png\" data-nickname=\"\u751f\u4fe1\u679c\" data-alias=\"shengxinguoer\" data-signature=\"\u751f\u4fe1\u5165\u95e8\u3001R\u8bed\u8a00\u3001\u751f\u4fe1\u56fe\u89e3\u8bfb\u4e0e\u7ed8\u5236\u3001\u8f6f\u4ef6\u64cd\u4f5c\u3001\u4ee3\u7801\u590d\u73b0\u3001\u751f\u4fe1\u786c\u6838\u77e5\u8bc6\u6280\u80fd\u3001\u670d\u52a1\u5668\u7b49\" data-from=\"2\" data-is_biz_ban=\"0\"><\/mp-common-profile><\/section>\n<section><br \/><\/section>\n<section style=\"white-space: normal;caret-color: rgba(0, 0, 0, 0);box-sizing: border-box;font-size: 16px;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 0.5em;box-sizing: border-box;\">\n<section style=\"margin-top: 0.3em;margin-bottom: 0.3em;height: 3px;background-color: rgb(0, 150, 136);box-sizing: border-box;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"text-align: left;box-sizing: border-box;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\">\u70b9\u51fb<strong style=\"box-sizing: border-box;\"><span style=\"color: rgb(0, 150, 136);box-sizing: border-box;\">\u201c\u9605\u8bfb\u539f\u6587\u201d<\/span><\/strong>\u7acb\u523b\u62e5\u6709<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"font-size: 32px;color: rgb(0, 150, 136);box-sizing: border-box;\">\u2193\u2193\u2193 <\/section>\n<\/section>\n<p><br style=\"white-space: normal;\" \/><\/p>\n<section><br \/><\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>scran\uff1a\u7ec6\u80de\u8868\u8fbe\u5927\u6570\u636e\u7684\u667a\u80fd\u5904\u7406\u5de5\u5177\uff0c\u52a9\u4f60\u6210\u4e3a\u8868\u8fbe\u5206\u6790\u4e13\u5bb6\uff01 \u539f\u521b \u5c0f\u679c 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{"id":47989,"date":"2024-06-15T20:07:58","date_gmt":"2024-06-15T12:07:58","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=47989"},"modified":"2024-06-15T20:07:58","modified_gmt":"2024-06-15T12:07:58","slug":"scran%ef%bc%9a%e7%bb%86%e8%83%9e%e8%a1%a8%e8%be%be%e5%a4%a7%e6%95%b0%e6%8d%ae%e7%9a%84%e6%99%ba%e8%83%bd%e5%a4%84%e7%90%86%e5%b7%a5%e5%85%b7%ef%bc%8c%e5%8a%a9%e4%bd%a0%e6%88%90%e4%b8%ba%e8%a1%a8-2","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=47989","title":{"rendered":"scran\uff1a\u7ec6\u80de\u8868\u8fbe\u5927\u6570\u636e\u7684\u667a\u80fd\u5904\u7406\u5de5\u5177\uff0c\u52a9\u4f60\u6210\u4e3a\u8868\u8fbe\u5206\u6790\u4e13\u5bb6\uff01"},"content":{"rendered":"

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center;justify-content: center;display: flex;flex-flow: row nowrap;box-sizing: border-box;\">\n<section style=\"padding-right: 12px;padding-left: 12px;display: inline-block;vertical-align: bottom;width: auto;background-color: rgb(147, 176, 108);min-width: 10%;max-width: 100%;flex: 0 0 auto;height: auto;align-self: flex-end;box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"font-size: 22px;color: rgb(255, 255, 255);text-align: justify;box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">1<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-left: 10px;display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;border-style: solid;border-width: 1px 0px;border-color: rgb(147, 176, 108) rgb(62, 62, 62);min-width: 10%;max-width: 100%;flex: 0 0 auto;height: auto;box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 4px;margin-bottom: 4px;box-sizing: border-box;\">\n<section style=\"color: rgb(147, 176, 108);text-align: justify;box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\">\u751f\u6210\u6570\u636e\u77e9\u9635<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\" style=\"box-sizing: border-box;\">\u4eca\u5929\u6211\u4eec\u7528\u5230\u7684\u6570\u636e\u4ecd\u7136\u6765\u81ea\u4e8escRNAseq\u5305\u4e2d\u7684\u7ec6\u80de\u96c6\u54e6\uff01\u6570\u636e\u77e9\u9635\u7684\u751f\u6210\u65b9\u6cd5\u5c0f\u679c\u5df2\u7ecf\u7ed9\u5927\u5bb6\u51c6\u5907\u597d\u5566\uff01\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\u5427\uff01<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">library(scRNAseq)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">data(fluidigm)<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">fluidigm<-ReprocessedFluidigmData()<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">ct <- <span class=\"code-snippet__built_in\" style=\"box-sizing: border-box;\">floor<\/span>(assays(fluidigm)$rsem_counts) #\u751f\u6210\u6570\u636e\u77e9\u9635<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">ct[<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">1<\/span>:<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">4<\/span>,<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">1<\/span>:<span class=\"code-snippet__number\" style=\"box-sizing: border-box;\">4<\/span>]<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">sample_ann <- as.data.frame(colData(fluidigm))<\/span><\/code><code style=\"box-sizing: border-box;\"><span class=\"code-snippet_outer\">DT::datatable(sample_ann)<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\"><br \/><\/p>\n<p style=\"box-sizing: border-box;\">\u4f7f\u7528R\u8bed\u8a00\u4e2d\u7684View()\u51fd\u6570\u53ef\u4ee5\u76f4\u63a5\u67e5\u770b\u6211\u4eec\u751f\u6210\u7684\u6570\u636e\u77e9\u9635\u54e6\uff0c\u6211\u4eec\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\u3002<\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.4649805447470817\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-05-06_scran\uff1a\u7ec6\u80de\u8868\u8fbe\u5927\u6570\u636e\u7684\u667a\u80fd\u5904\u7406\u5de5\u5177\uff0c\u52a9\u4f60\u6210\u4e3a\u8868\u8fbe\u5206\u6790\u4e13\u5bb6\uff01\/2_QXIaWm7icdSRAkbLhHhryBjuyOibNw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAwtJh5WPmw9Q70nJCdPAypjdTIuT71XzISibRWHfc7aNhnbuO7QXIaWm7icdSRAkbLhHhryBjuyOibNw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"514\"><\/p>\n<p style=\"box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;box-sizing: border-box;\">\n<section style=\"padding-right: 12px;padding-left: 12px;display: inline-block;vertical-align: bottom;width: auto;background-color: rgb(147, 176, 108);min-width: 10%;max-width: 100%;flex: 0 0 auto;height: auto;align-self: flex-end;box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"font-size: 22px;color: rgb(255, 255, 255);text-align: justify;box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\">2<br style=\"box-sizing: border-box;\" \/><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-left: 10px;display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;border-style: solid;border-width: 1px 0px;border-color: rgb(147, 176, 108) rgb(62, 62, 62);min-width: 10%;max-width: 100%;flex: 0 0 auto;height: auto;box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 4px;margin-bottom: 4px;box-sizing: border-box;\">\n<section style=\"color: rgb(147, 176, 108);text-align: justify;box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\">\u63a2\u7d22\u57fa\u56e0\u8868\u8fbe\u60c5\u51b5<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\">\u5728\u63a2\u7d22\u57fa\u56e0\u8868\u8fbe\u60c5\u51b5\uff0c\u5177\u4f53\u65f6\u4ece\u54ea\u4e9b\u89d2\u5ea6\u6765\u770b\u5462\uff1f\u5c0f\u679c\u5728\u8fd9\u91cc\u7ed9\u5927\u5bb6\u4f53\u5171\u4e24\u79cd\u601d\u8def\uff1a<\/p>\n<p style=\"box-sizing: border-box;\">1.\u7edf\u8ba1\u67d0\u4e2a\u57fa\u56e0\u5728\u591a\u5c11\u4e2a\u7ec6\u80de\u4e2d\u5f97\u5230\u8868\u8fbe\uff1b<\/p>\n<p style=\"box-sizing: border-box;\">2.\u7edf\u8ba1\u4e00\u4e2a\u7ec6\u80de\u4e2d\u8868\u8fbe\u4e86\u591a\u5c11\u4e2a\u57fa\u56e0\uff1b<\/p>\n<p style=\"box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<p style=\"box-sizing: border-box;\">\u90a3\u4e48\u8981\u4ece\u8fd9\u4e24\u4e2a\u89d2\u5ea6\u53bb\u63a2\u7d22\uff0c\u5206\u522b\u8981\u5982\u4f55\u53bb\u7edf\u8ba1\u5462\uff1f\u5feb\u8c03\u52a8\u4f60\u806a\u660e\u7684\u5c0f\u8111\u888b\uff01\u5982\u679c\u60f3\u4e0d\u5230\u4e5f\u6ca1\u5173\u7cfb\uff0c\u4eca\u5929\u5c0f\u679c\u624b\u628a\u624b\u6559\u4f60\u5b8c\u6210\uff01<\/p>\n<p style=\"box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<p style=\"box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">o \u7edf\u8ba1\u6240\u6709\u57fa\u56e0\u8868\u8fbe\u6210\u529f\u7684\u7ec6\u80de\u4e2a\u6570<\/strong><\/p>\n<p style=\"box-sizing: border-box;\">\u5bf9\u4e8e\u57fa\u56e0\u8868\u8fbe\u6210\u529f\u7684\u7ec6\u80de\u4e2a\u6570\uff0c\u6211\u4eec\u9700\u8981\u7528apply\u51fd\u6570\u7edf\u8ba1\u6570\u636e\u77e9\u9635\u4e2d\u6bcf\u4e00\u884c\u5143\u7d20\u4e2d\u5bf9\u5e94\u503c\u5927\u4e8e0\u7684\u4e2a\u6570\uff0c\u63a5\u4e0b\u6765\u6211\u4eec\u8fdb\u884c\u7edf\u8ba1\u5e76\u7ed8\u5236\u7bb1\u7ebf\u56fe\u89c2\u5bdf\u6574\u4f53\u8868\u8fbe\u60c5\u51b5\u3002\u548c\u5c0f\u679c\u4e00\u8d77\u6765\u770b\u770b\u5427\uff01<\/p>\n<p style=\"box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<p style=\"box-sizing: border-box;\"><span style=\"color: rgb(136, 136, 136);\">fivenum(apply(counts,1,function(x) sum(x>0) ))#\u7edf\u8ba1\u6240\u6709\u57fa\u56e0\u8868\u8fbe\u6210\u529f\u7ec6\u80de\u4e2a\u6570\u4e2d\u7684\u4e94\u4e2a\u6307\u6807<br style=\"box-sizing: border-box;\" \/>boxplot(apply(counts,1,function(x) sum(x>0) ))#\u7ed8\u5236\u7bb1\u7ebf\u56fe<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.574757281553398\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-05-06_scran\uff1a\u7ec6\u80de\u8868\u8fbe\u5927\u6570\u636e\u7684\u667a\u80fd\u5904\u7406\u5de5\u5177\uff0c\u52a9\u4f60\u6210\u4e3a\u8868\u8fbe\u5206\u6790\u4e13\u5bb6\uff01\/3_XWnOIJzejwOI236LR2I0Cpic0TEXDw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAwtJh5WPmw9Q70nJCdPAypjdvefqJwbc5c9lrSKdIcdHpnZ2XWnOIJzejwOI236LR2I0Cpic0TEXDw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"515\"><\/p>\n<p style=\"box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<p style=\"box-sizing: border-box;\">\u5f97\u5230\u4ee5\u4e0a\u7684\u7bb1\u7ebf\u56fe\u540e\uff0c\u5c0f\u679c\u6765\u7ed9\u5927\u5bb6\u505a\u4e00\u4e2a\u7b80\u5355\u7684\u89e3\u8bfb\u5427\uff01\u7531\u7bb1\u7ebf\u56fe\u7684\u4e2d\u4f4d\u7ebf\u53ef\u4ee5\u770b\u51fa\uff0c\u57fa\u56e0\u8868\u8fbe\u6210\u529f\u7684\u7ec6\u80de\u6570\u7684\u4e2d\u4f4d\u6570\u503c\u5927\u6982\u57283\u4e2a\u5de6\u53f3\uff0c\u8868\u8fbe\u7ec6\u80de\u6570\u7684\u9608\u503c\u5927\u6982\u572835\u5de6\u53f3\uff0c\u5373\u8bf4\u660e\u5982\u679c\u57fa\u56e0\u572835\u4e2a\u7ec6\u80de\u5185\u4e0d\u8868\u8fbe\u5c31\u53ef\u4ee5\u76f4\u63a5\u8fc7\u6ee4\u6389\u54e6\u3002<\/p>\n<p style=\"box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<p style=\"box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">o \u7edf\u8ba1\u6240\u6709\u7ec6\u80de\u8868\u8fbe\u4e86\u7684\u57fa\u56e0\u4e2a\u6570<\/strong><\/p>\n<p style=\"box-sizing: border-box;\">\u6709\u4e86\u4e0a\u9762\u7684\u57fa\u7840\uff0c\u4f60\u662f\u5426\u5b66\u4f1a\u4e3e\u4e00\u53cd\u4e09\u4e86\u5462\uff1f\u5bf9\u4e8e\u7ec6\u80de\u8868\u8fbe\u4e86\u7684\u57fa\u56e0\u4e2a\u6570\uff0c\u6211\u4eec\u540c\u6837\u4f7f\u7528apply\u51fd\u6570\u7edf\u8ba1\u6570\u636e\u77e9\u9635\u4e2d\u7684\u6bcf\u4e00\u5217\u5143\u7d20\u4e2d\u5bf9\u5e94\u503c\u5927\u4e8e0\u7684\u4e2a\u6570\u5373\u53ef\uff0c\u63a5\u4e0b\u6765\u6211\u4eec\u7528\u76f4\u65b9\u5206\u5e03\u56fe\u6765\u548c\u5927\u5bb6\u5c55\u793a\u4e00\u4e0b\u6574\u4f53\u7684\u8868\u8fbe\u60c5\u51b5\u3002\u548c\u5c0f\u679c\u4e00\u8d77\u770b\u770b\u5427\uff01<\/p>\n<p style=\"box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<p style=\"box-sizing: border-box;\"><span style=\"color: rgb(136, 136, 136);\">fivenum(apply(counts,2,function(x) sum(x>0) ))#\u7edf\u8ba1\u6240\u6709\u7ec6\u80de\u8868\u8fbe\u4e86\u57fa\u56e0\u4e2a\u6570\u4e2d\u7684\u4e94\u4e2a\u6307\u6807<br style=\"box-sizing: border-box;\" \/>hist(apply(counts,2,function(x) sum(x>0) )) #\u7ed8\u5236\u76f4\u65b9\u5206\u5e03\u56fe<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.7113970588235294\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-05-06_scran\uff1a\u7ec6\u80de\u8868\u8fbe\u5927\u6570\u636e\u7684\u667a\u80fd\u5904\u7406\u5de5\u5177\uff0c\u52a9\u4f60\u6210\u4e3a\u8868\u8fbe\u5206\u6790\u4e13\u5bb6\uff01\/4_Diae8vA44hOJbXgAMtdc63AbqLlW0g.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAwtJh5WPmw9Q70nJCdPAypja1YdelzYblFCkvWviaWUhTTWTuDiae8vA44hOJbXgAMtdc63AbqLlW0g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"544\"><span style=\"text-align: justify;\"><\/span><\/p>\n<p style=\"box-sizing: border-box;\"> \u5728\u5206\u6790\u4e86\u7bb1\u7ebf\u56fe\u7684\u7edf\u8ba1\u7ed3\u679c\u540e\uff0c\u4f60\u80fd\u5426\u72ec\u7acb\u5206\u6790\u76f4\u65b9\u56fe\u7684\u7edf\u8ba1\u7ed3\u679c\u5462\uff1f\u6211\u4eec\u8fd8\u662f\u548c\u5c0f\u679c\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\uff01<\/p>\n<p style=\"box-sizing: border-box;\"> \u7531\u76f4\u65b9\u5206\u5e03\u56fe\u7684\u7edf\u8ba1\u7ed3\u679c\u53ef\u4ee5\u770b\u51fa\uff0c\u8868\u8fbe\u57fa\u56e0\u4e2a\u6570\u57283000~4000\u4e4b\u95f4\u7684\u7ec6\u80de\u5360\u6700\u591a\u6570\u91cf\uff0c\u8868\u8fbe\u57fa\u56e0\u4e2a\u6570\u57288000\u4ee5\u4e0a\u7684\u7ec6\u80de\u4ec5\u67092~3\u4e2a\uff0c\u5360\u6700\u5c11\u7684\u6570\u91cf\u3002<\/p>\n<p style=\"box-sizing: border-box;\"> \u901a\u8fc7\u4ee5\u4e0a\u4e24\u7ec4\u7edf\u8ba1\u56fe\u7684\u5206\u6790\uff0c\u6211\u4eec\u5c31\u5bf9\u57fa\u56e0\u5728\u7ec6\u80de\u4e2d\u7684\u8868\u8fbe\u60c5\u51b5\u6e38\u5ba2\u6574\u4f53\u7684\u628a\u63e1\uff0c\u600e\u4e48\u6837\uff0c\u4eca\u5929\u7684\u5c0f\u6280\u5de7\u4f60\u5b66\u5e9f\u4e86\u561b\uff1f\u66f4\u591a\u751f\u4fe1\u6280\u5de7\u8bf7\u7ee7\u7eed\u5173\u6ce8\u5c0f\u679c\u54e6\uff01<\/p>\n<\/section>\n<p style=\"box-sizing: border-box;\"><br \/><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;box-sizing: border-box;\">\n<section style=\"display: inline-block;vertical-align: top;box-sizing: border-box;\">\n<section style=\"padding-right: 12px;padding-left: 12px;background-color: rgb(255, 255, 255);box-sizing: border-box;\">\n<p style=\"clear: none;box-sizing: border-box;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;font-size: 16px;white-space: normal;box-sizing: border-box;\">\n<section style=\"box-sizing: border-box;\">\n<section>\n<section style=\"box-sizing: border-box;max-width: 100%;\">\n<section>\n<section style=\"box-sizing: border-box;max-width: 100%;\">\n<section>\n<section style=\"box-sizing: border-box;max-width: 100%;\">\n<section>\n<section style=\"box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"box-sizing: border-box;\">\n<section style=\"line-height: 1.8;box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"box-sizing: border-box;\">\n<section style=\"box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 20px;display: flex;flex-flow: row nowrap;text-align: center;justify-content: center;box-sizing: border-box;\">\n<section style=\"display: inline-block;width: 543.108px;vertical-align: top;border-style: solid;border-width: 1px;border-color: rgb(67, 67, 67);flex: 0 0 auto;align-self: flex-start;height: auto;box-shadow: rgb(255, 219, 118) 6px 6px 0px;background-color: rgb(255, 255, 255);box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;box-sizing: border-box;\">\n<section style=\"display: inline-block;width: 378.611px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;box-sizing: border-box;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;box-sizing: border-box;\">\n<section style=\"text-align: center;box-sizing: border-box;\">\n<p style=\"box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\"><span style=\"font-size: 15px;box-sizing: border-box;\">\u751f\u4fe1\u4ebaR\u8bed\u8a00\u5b66\u4e60\u5fc5\u5907<\/span><\/strong><\/p>\n<p style=\"box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\"><span style=\"font-size: 15px;box-sizing: border-box;\">\u7acb\u523b\u62e5\u6709\u4e00\u4e2aRstudio\u8d26\u53f7<\/span><\/strong><\/p>\n<p style=\"box-sizing: 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