<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 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#e7e7eb;}@supports(-webkit-overflow-scrolling:touch){.rich_media_title{font-weight:700}}.rich_media_meta{display:inline-block;vertical-align:middle;padding:0 0 10px 0;font-size:15px;-webkit-tap-highlight-color:rgba(0,0,0,0)}.rich_media_meta.icon_appmsg_tag{margin-right:0px}.rich_media_meta.meta_tag_text{margin-right:0}.rich_media_meta_list em{font-style:normal}.rich_media_meta_text{color:#a5a5a5;}p{margin:0;}.msgBox{margin-top:20px;padding-top:20px;padding-left:50px;overflow:hidden;border-top:2px dashed #09a2ff;}.msg{padding-top:7px;clear:both;}.msgBody{float:right;width:100%;margin-left:55px;padding-bottom:15px;border-bottom:1px dashed #e0e0e0;}.userHeadImg{float:left;margin-left:-50px;}.userHeadImg img{width:40px;height:40px;margin-right:10px;border-radius:3px;}.userName{color:#888888;line-height:24px;font-size:14px;margin:5px 0 5px 0;height:24px;}.replyBody,.autherBody{color:#565656;font-size:15px;}.replyIcon{border-left:4px solid #33ab01;margin-right:5px;}.ad{text-decoration:none;color:#d6d4d4;font-size:12px;}.msgBodyReply{padding-top:5px;}.userName span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation Mono\",Menlo,Courier,monospace}.code-snippet__comment,.code-snippet__quote{color:#afafaf;font-style:italic}.code-snippet__keyword,.code-snippet__selector-tag,.code-snippet__subst{color:#ca7d37}.code-snippet__number,.code-snippet__literal,.code-snippet__variable,.code-snippet__template-variable,.code-snippet__tag .code-snippet__attr{color:#0e9ce5}.code-snippet__string,.code-snippet__doctag{color:#d14}.code-snippet__title,.code-snippet__section,.code-snippet__selector-id{color:#d14}.code-snippet__subst{font-weight:normal}.code-snippet__type,.code-snippet__class .code-snippet__title{color:#0e9ce5}.code-snippet__tag,.code-snippet__name,.code-snippet__attribute{color:#0e9ce5;font-weight:normal}.code-snippet__regexp,.code-snippet__link{color:#ca7d37}.code-snippet__symbol,.code-snippet__bullet{color:#d14}.code-snippet__built_in,.code-snippet__builtin-name{color:#ca7d37}.code-snippet__meta{color:#afafaf}.code-snippet__deletion{background:#fdd}.code-snippet__addition{background:#dfd}.code-snippet__emphasis{font-style:italic}.code-snippet__strong{font-weight:bold}.account_avatar{width:40px;height:40px;padding:0;}.account_info{display:-webkit-box;display:-webkit-flex;display:flex;-webkit-box-align:center;-webkit-align-items:center;padding:20px 0;align-items:center}.flex_bd{padding-left:14px;}.account_nickname{display:inline-block;vertical-align:middle;line-height:1.2;color:#576b95;font-size:14px}.account_desc{overflow:hidden;text-overflow:ellipsis;display:-webkit-box;-webkit-box-orient:vertical;-webkit-line-clamp:1;color:rgba(0,0,0,0.3);font-size:14px;line-height:1.2;padding-top:.4em}.msg_source_url{text-align:left;word-break:break-all;margin-top:20px;}.msg_source_url a{padding-right:10px;}.msg_source_url .url_text{color:#a8a8a8;}.video-desc{font-size:14px;margin-top:15px;color:#6c6c6c;}.msg_source_url{text-align:left;}.original_primary_card_tips{color:rgba(0,0,0,0.3);line-height:1.4;font-size:15px;}.weui-flex__item{margin-bottom:20px;padding:20px 16px;margin-top:16px;line-height:1.4;align-items:center;background-color:#f7f7f7;border-radius:8px;position:relative;}.original_primary_desc{color:rgba(0,0,0,0.5);font-size:14px;padding-top:4px;width:auto;overflow:hidden;text-overflow:ellipsis;}.msgBodyReplyList{border-top:1px solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"\u5c0f\u679c\u6559\u4f60\u7528TransDecoder\u8f7b\u677e\u9884\u6d4b\u86cb\u767d\u5f00\u653e\u9605\u8bfb\u6846\u5e8f\u5217\",\"time\":\"2022-12-03 19:00:28\",\"timeStamp\":\"1670065228\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247483976&idx=1&sn=2cfa9de54c5cc4e438741a314d84d75d&chksm=cf7c928df80b1b9bbf55a81bc1d7ddda54c8ad6330af9c756e09e754d285ac561c28dab043e0#rd\" target=\"_blank\" rel=\"noopener\">\u5c0f\u679c\u6559\u4f60\u7528TransDecoder\u8f7b\u677e\u9884\u6d4b\u86cb\u767d\u5f00\u653e\u9605\u8bfb\u6846\u5e8f\u5217<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span id=\"copyright_logo\" class=\"wx_tap_link js_wx_tap_highlight rich_media_meta icon_appmsg_tag appmsg_title_tag weui-wa-hotarea\">\u539f\u521b <\/span><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u679c <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2022-12-03 19:00:28<\/em><\/div>\n<p><content><\/p>\n<div class=\"topic\">\n<p>\u6536\u5f55\u4e8e\u8bdd\u9898<\/p>\n<p><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2668999206111641602#wechat_redirect\" title=\"\u6570\u636e\u5e93\" target=\"_blank\" rel=\"noopener\">#\u6570\u636e\u5e93<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2664545260517294084#wechat_redirect\" title=\"\u751f\u4fe1\u5b9e\u64cd\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u5b9e\u64cd<\/a><\/p>\n<\/div>\n<p style=\"text-align: center;\" data-mpa-powered-by=\"yiban.io\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.18518518518518517\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_YHrH5kBzN1unDT7eHqLtdZO1vwdtrw-30.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/zcicibWZessAxZibD6iaowp2Zv5dgyZQqBVAac9npnibJvGYo1ZFiafpiayXgbaYHrH5kBzN1unDT7eHqLtdZO1vwdtrw\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<section style=\"box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<section style=\"box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;box-sizing: border-box;\">\u5c0f\u679c\u4e4b\u524d\u4e3b\u8981\u662f\u5728\u5904\u7406\u6570\u636e\u6316\u6398\uff0c\u4ece\u516c\u5171\u6570\u636e\u5e93\u91cc\u627e\u73b0\u6210\u7684\u8868\u8fbe\u77e9\u9635\u3002\u4f46\u5c0f\u679c\u4eca\u5929\u63a5\u5230\u4e2a\u8bdd\uff0c\u8981\u5904\u7406\u57fa\u56e0\u5e8f\u5217\uff0c\u5c31\u662f\u6709\u4e00\u4e2a\u8f6c\u5f55\u672c\u7684fasta\u5e8f\u5217\u6587\u4ef6\uff0c\u9700\u8981\u9884\u6d4b\u4e00\u4e0b\u5b83\u7684\u86cb\u767d\u5e8f\u5217\uff0c\u627e\u4e00\u4e0b\u5f00\u653e\u9605\u8bfb\u6846\uff0c\u8fd9\u4e2a\u64cd\u4f5c\u5176\u5b9e\u4e0d\u7b97\u590d\u6742\uff0c\u6709\u4e2a\u8f6f\u4ef6\u5c31\u884c\u4e86\uff0c\u5c31\u662f<strong style=\"box-sizing: border-box;\">TransDecoder<\/strong>\u3002<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\"><br \/><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">\u5148\u8bf4\u4e00\u4e0b\uff0c\u8fd9\u662flinux\u7cfb\u7edf\u4e0b\u7684\u8f6f\u4ef6\uff0c\u6240\u4ee5\u6211\u4eec\u7684\u64cd\u4f5c\u8981\u5728linux\u7cfb\u7edf\u4e0b\u8fdb\u884c\uff0c\u9996\u5148\u662f\u8981\u5b89\u88c5\u8f6f\u4ef6\uff0c\u8fd9\u4e2a\u8f6f\u4ef6\u5176\u5b9e\u8fd8\u662f\u633a\u53cb\u597d\u7684\uff0c\u56e0\u4e3a\u4ed6\u53ea\u8981\u4e0b\u8f7d\u4e0b\u6765\u5c31\u884c\uff0c\u4e0d\u9700\u8981\u5355\u72ec\u5b89\u88c5\u3002\u53ef\u4ee5\u5728linux\u7cfb\u7edf\u4e0b\u76f4\u63a5\u4f7f\u7528\u4e0b\u9762\u8fd9\u4e2a\u547d\u4ee4\u3002<\/p>\n<p style=\"text-align: left;white-space: normal;box-sizing: border-box;\">wget https:\/\/codeload.github.com\/TransDecoder\/TransDecoder\/tar.gz\/2.0.1<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">\u4e5f\u53ef\u4ee5\u5148\u4e0b\u8f7d\u4e0b\u6765\u7136\u540e\u518d\u4f20\u8f93\u5230linux\u7cfb\u7edf\u91cc\u3002<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\"><br \/><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">\u7b80\u5355\u8bf4\u4e00\u4e0bfasta\u6587\u4ef6\uff0c\u5c31\u662f\u4e00\u884c\u6807\u9898\uff0c\u7136\u540e\u4e0b\u9762\u4e00\u884c\u6216\u51e0\u884c\u5e8f\u5217<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"max-width: 100%;vertical-align: middle;display: inline-block;line-height: 0;box-sizing: border-box;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6921529\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_eRdRPeBWWJ35BMXGLcBodt9iaricfA-2.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAyqZ8j1Z8oWvYiciabcfQK3H9gVPQUWsQK807xjh4Yj7rdaXBodeRdRPeBWWJ35BMXGLcBodt9iaricfA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"497\" style=\"vertical-align: middle;width: 100%;box-sizing: border-box;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section style=\"box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">TransDecoder.LongOrfs -t target_transcripts.fasta<\/strong><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\"><br \/><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">\u9ed8\u8ba4\u60c5\u51b5\u4e0b\uff0cTransDecoder.LongOrfs\u5c06\u8bc6\u522b\u81f3\u5c11100\u4e2a\u6c28\u57fa\u9178\u957f\u7684ORF\u3002\u53ef\u4ee5\u901a\u8fc7\u2019-m\u2019\u53c2\u6570\u964d\u4f4e\u6b64\u503c\uff0c\u4f46\u53ef\u4ee5\u77e5\u9053\uff0c\u4f7f\u7528\u66f4\u77ed\u7684\u6700\u5c0f\u957f\u5ea6\u6807\u51c6\uff0c\u8bef\u62a5ORF\u9884\u6d4b\u7684\u6bd4\u7387\u4f1a\u6025\u5267\u589e\u52a0<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\"><br \/><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">TransDecoder.Predict -t target_transcripts.fasta<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">\u6700\u7ec8\u7684\u6587\u4ef6\u53ef\u4ee5\u5728\u5f53\u524d\u76ee\u5f55\u627e\u5230\uff0c\u4e5f\u5c31\u662f\u540e\u7f00\u4e3a.pep, .cds, .gff3\u548c.bed\u7684\u6587\u4ef6<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\"><br \/><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">\u4e00\u822c\u6765\u8bf4\uff0c\u53ef\u4ee5\u4f7f\u7528TransDecoder\u5bf9\u65e0\u53c2\u8f6c\u5f55\u7ec4\u7684\u62fc\u63a5\u7ed3\u679c\u5e8f\u5217\u9884\u6d4b\u5176CDS\uff0c\u6240\u4ee5\u6211\u4eec\u53ef\u4ee5\u5148\u5c06\u62fc\u63a5\u5e8f\u5217\u7528BLAST\u6bd4\u5bf9nr\u4ee5\u53caswissprot\u86cb\u767d\u6570\u636e\u5e93\uff0c\u7136\u540e\u63d0\u53d6\u5176\u6bd4\u5bf9\u4e0a\u7684\u540c\u6e90\u5e8f\u5217\u7684\u4f4d\u7f6e\u6765\u8bc6\u522bCDS\uff0c\u6700\u540e\u518d\u901a\u8fc7TransDecoder\u7684\u7b2c\u4e00\u6b65\u548c\u7b2c\u4e09\u6b65\u6765\u9884\u6d4b\u90a3\u4e9b\u672a\u6bd4\u5bf9\u4e0a\u7684\u5e8f\u5217\u7684CDS\u3002\u8fd9\u4e2a\u5c0f\u4f19\u4f34\u4eec\u4e86\u89e3\u4e00\u4e0b\u5c31\u597d\u3002<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<\/section>\n<p style=\"white-space: normal;box-sizing: border-box;\" powered-by=\"xiumi.us\">\u8f6f\u4ef6\u4f1a\u521b\u5efa\u4e00\u4e2a\u5de5\u4f5c\u76ee\u5f55(\u4f8b\u5982transcripts.transdecoder_dir \/)\u4fdd\u5b58\u7ed3\u679c\uff0c\u5176\u4e2d\u5305\u542b\uff1a<\/p>\n<section style=\"margin-top: 10px;margin-bottom: 10px;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;border-width: 2px;border-style: dotted;border-color: rgb(192, 200, 209);padding: 10px;box-sizing: border-box;\">\n<section style=\"box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;box-sizing: border-box;\">longest_orfs.pep: \u6ee1\u8db3\u6700\u5c0f\u957f\u5ea6\u6807\u51c6\u7684\u6240\u6709ORF\uff0c\u65e0\u8bba\u7f16\u7801\u6f5c\u529b\u5982\u4f55\u3002<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">longest_orfs.gff3: \u76ee\u6807\u8f6c\u5f55\u672c\u4e2d\u6240\u6709ORF\u7684\u4f4d\u7f6e<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">longest_orfs.cds: 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solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"\u5c0f\u679c\u6559\u4f60\u7528TransDecoder\u8f7b\u677e\u9884\u6d4b\u86cb\u767d\u5f00\u653e\u9605\u8bfb\u6846\u5e8f\u5217\",\"time\":\"2022-12-03 19:00:28\",\"timeStamp\":\"1670065228\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247483976&idx=1&sn=2cfa9de54c5cc4e438741a314d84d75d&chksm=cf7c928df80b1b9bbf55a81bc1d7ddda54c8ad6330af9c756e09e754d285ac561c28dab043e0#rd\" target=\"_blank\" rel=\"noopener\">\u5c0f\u679c\u6559\u4f60\u7528TransDecoder\u8f7b\u677e\u9884\u6d4b\u86cb\u767d\u5f00\u653e\u9605\u8bfb\u6846\u5e8f\u5217<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span id=\"copyright_logo\" class=\"wx_tap_link js_wx_tap_highlight rich_media_meta icon_appmsg_tag appmsg_title_tag weui-wa-hotarea\">\u539f\u521b <\/span><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u679c <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2022-12-03 19:00:28<\/em><\/div>\n<p><content><\/p>\n<div class=\"topic\">\n<p>\u6536\u5f55\u4e8e\u8bdd\u9898<\/p>\n<p><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2668999206111641602#wechat_redirect\" title=\"\u6570\u636e\u5e93\" target=\"_blank\" rel=\"noopener\">#\u6570\u636e\u5e93<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2664545260517294084#wechat_redirect\" title=\"\u751f\u4fe1\u5b9e\u64cd\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u5b9e\u64cd<\/a><\/p>\n<\/div>\n<p style=\"text-align: center;\" data-mpa-powered-by=\"yiban.io\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.18518518518518517\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_YHrH5kBzN1unDT7eHqLtdZO1vwdtrw-30.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/zcicibWZessAxZibD6iaowp2Zv5dgyZQqBVAac9npnibJvGYo1ZFiafpiayXgbaYHrH5kBzN1unDT7eHqLtdZO1vwdtrw\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<section style=\"box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<section style=\"box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;box-sizing: border-box;\">\u5c0f\u679c\u4e4b\u524d\u4e3b\u8981\u662f\u5728\u5904\u7406\u6570\u636e\u6316\u6398\uff0c\u4ece\u516c\u5171\u6570\u636e\u5e93\u91cc\u627e\u73b0\u6210\u7684\u8868\u8fbe\u77e9\u9635\u3002\u4f46\u5c0f\u679c\u4eca\u5929\u63a5\u5230\u4e2a\u8bdd\uff0c\u8981\u5904\u7406\u57fa\u56e0\u5e8f\u5217\uff0c\u5c31\u662f\u6709\u4e00\u4e2a\u8f6c\u5f55\u672c\u7684fasta\u5e8f\u5217\u6587\u4ef6\uff0c\u9700\u8981\u9884\u6d4b\u4e00\u4e0b\u5b83\u7684\u86cb\u767d\u5e8f\u5217\uff0c\u627e\u4e00\u4e0b\u5f00\u653e\u9605\u8bfb\u6846\uff0c\u8fd9\u4e2a\u64cd\u4f5c\u5176\u5b9e\u4e0d\u7b97\u590d\u6742\uff0c\u6709\u4e2a\u8f6f\u4ef6\u5c31\u884c\u4e86\uff0c\u5c31\u662f<strong style=\"box-sizing: border-box;\">TransDecoder<\/strong>\u3002<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\"><br \/><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">\u5148\u8bf4\u4e00\u4e0b\uff0c\u8fd9\u662flinux\u7cfb\u7edf\u4e0b\u7684\u8f6f\u4ef6\uff0c\u6240\u4ee5\u6211\u4eec\u7684\u64cd\u4f5c\u8981\u5728linux\u7cfb\u7edf\u4e0b\u8fdb\u884c\uff0c\u9996\u5148\u662f\u8981\u5b89\u88c5\u8f6f\u4ef6\uff0c\u8fd9\u4e2a\u8f6f\u4ef6\u5176\u5b9e\u8fd8\u662f\u633a\u53cb\u597d\u7684\uff0c\u56e0\u4e3a\u4ed6\u53ea\u8981\u4e0b\u8f7d\u4e0b\u6765\u5c31\u884c\uff0c\u4e0d\u9700\u8981\u5355\u72ec\u5b89\u88c5\u3002\u53ef\u4ee5\u5728linux\u7cfb\u7edf\u4e0b\u76f4\u63a5\u4f7f\u7528\u4e0b\u9762\u8fd9\u4e2a\u547d\u4ee4\u3002<\/p>\n<p style=\"text-align: left;white-space: normal;box-sizing: border-box;\">wget https:\/\/codeload.github.com\/TransDecoder\/TransDecoder\/tar.gz\/2.0.1<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">\u4e5f\u53ef\u4ee5\u5148\u4e0b\u8f7d\u4e0b\u6765\u7136\u540e\u518d\u4f20\u8f93\u5230linux\u7cfb\u7edf\u91cc\u3002<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\"><br \/><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">\u7b80\u5355\u8bf4\u4e00\u4e0bfasta\u6587\u4ef6\uff0c\u5c31\u662f\u4e00\u884c\u6807\u9898\uff0c\u7136\u540e\u4e0b\u9762\u4e00\u884c\u6216\u51e0\u884c\u5e8f\u5217<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"max-width: 100%;vertical-align: middle;display: inline-block;line-height: 0;box-sizing: border-box;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6921529\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_eRdRPeBWWJ35BMXGLcBodt9iaricfA-2.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAyqZ8j1Z8oWvYiciabcfQK3H9gVPQUWsQK807xjh4Yj7rdaXBodeRdRPeBWWJ35BMXGLcBodt9iaricfA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"497\" style=\"vertical-align: middle;width: 100%;box-sizing: border-box;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section style=\"box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">TransDecoder.LongOrfs -t target_transcripts.fasta<\/strong><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\"><br \/><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">\u9ed8\u8ba4\u60c5\u51b5\u4e0b\uff0cTransDecoder.LongOrfs\u5c06\u8bc6\u522b\u81f3\u5c11100\u4e2a\u6c28\u57fa\u9178\u957f\u7684ORF\u3002\u53ef\u4ee5\u901a\u8fc7\u2019-m\u2019\u53c2\u6570\u964d\u4f4e\u6b64\u503c\uff0c\u4f46\u53ef\u4ee5\u77e5\u9053\uff0c\u4f7f\u7528\u66f4\u77ed\u7684\u6700\u5c0f\u957f\u5ea6\u6807\u51c6\uff0c\u8bef\u62a5ORF\u9884\u6d4b\u7684\u6bd4\u7387\u4f1a\u6025\u5267\u589e\u52a0<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\"><br \/><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">TransDecoder.Predict -t target_transcripts.fasta<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">\u6700\u7ec8\u7684\u6587\u4ef6\u53ef\u4ee5\u5728\u5f53\u524d\u76ee\u5f55\u627e\u5230\uff0c\u4e5f\u5c31\u662f\u540e\u7f00\u4e3a.pep, .cds, .gff3\u548c.bed\u7684\u6587\u4ef6<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\"><br \/><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">\u4e00\u822c\u6765\u8bf4\uff0c\u53ef\u4ee5\u4f7f\u7528TransDecoder\u5bf9\u65e0\u53c2\u8f6c\u5f55\u7ec4\u7684\u62fc\u63a5\u7ed3\u679c\u5e8f\u5217\u9884\u6d4b\u5176CDS\uff0c\u6240\u4ee5\u6211\u4eec\u53ef\u4ee5\u5148\u5c06\u62fc\u63a5\u5e8f\u5217\u7528BLAST\u6bd4\u5bf9nr\u4ee5\u53caswissprot\u86cb\u767d\u6570\u636e\u5e93\uff0c\u7136\u540e\u63d0\u53d6\u5176\u6bd4\u5bf9\u4e0a\u7684\u540c\u6e90\u5e8f\u5217\u7684\u4f4d\u7f6e\u6765\u8bc6\u522bCDS\uff0c\u6700\u540e\u518d\u901a\u8fc7TransDecoder\u7684\u7b2c\u4e00\u6b65\u548c\u7b2c\u4e09\u6b65\u6765\u9884\u6d4b\u90a3\u4e9b\u672a\u6bd4\u5bf9\u4e0a\u7684\u5e8f\u5217\u7684CDS\u3002\u8fd9\u4e2a\u5c0f\u4f19\u4f34\u4eec\u4e86\u89e3\u4e00\u4e0b\u5c31\u597d\u3002<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<\/section>\n<p style=\"white-space: normal;box-sizing: border-box;\" powered-by=\"xiumi.us\">\u8f6f\u4ef6\u4f1a\u521b\u5efa\u4e00\u4e2a\u5de5\u4f5c\u76ee\u5f55(\u4f8b\u5982transcripts.transdecoder_dir \/)\u4fdd\u5b58\u7ed3\u679c\uff0c\u5176\u4e2d\u5305\u542b\uff1a<\/p>\n<section style=\"margin-top: 10px;margin-bottom: 10px;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;border-width: 2px;border-style: dotted;border-color: rgb(192, 200, 209);padding: 10px;box-sizing: border-box;\">\n<section style=\"box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;box-sizing: border-box;\">longest_orfs.pep: \u6ee1\u8db3\u6700\u5c0f\u957f\u5ea6\u6807\u51c6\u7684\u6240\u6709ORF\uff0c\u65e0\u8bba\u7f16\u7801\u6f5c\u529b\u5982\u4f55\u3002<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">longest_orfs.gff3: \u76ee\u6807\u8f6c\u5f55\u672c\u4e2d\u6240\u6709ORF\u7684\u4f4d\u7f6e<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">longest_orfs.cds: \u6240\u6709\u68c0\u6d4b\u5230\u7684ORF\u7684\u6838\u82f7\u9178\u7f16\u7801\u5e8f\u5217<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">longest_orfs.cds.top_500_longest: top 500\u6700\u957f\u7684ORF\uff0c\u7528\u4e8e\u8bad\u7ec3\u7f16\u7801\u5e8f\u5217\u7684\u9a6c\u5c14\u53ef\u592b\u6a21\u578b<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">\u4ee5\u4e0a\u662fTransDecoder.LongOrfs\u7684\u7ed3\u679c\uff0c\u4e0b\u9762\u662fTransDecoder.Predict\u7684\u9884\u6d4b\u7ed3\u679c\u3002<\/strong><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">hexamer.scores: \u6bcf\u4e2ak-mer\u7684\u5bf9\u6570\u4f3c\u7136\u5206\u6570\uff08\u7f16\u7801\/\u968f\u673a\uff09<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">longest_orfs.cds.scores: 6\u4e2a\u9605\u8bfb\u6846\u4e2d\u6bcf\u4e2aORF\u7684\u5bf9\u6570\u4f3c\u7136\u548c\u5206\u6570<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">longest_orfs.cds.scores.selected: \u6839\u636e\u8bc4\u5206\u6807\u51c6\u9009\u62e9\u7684ORF\u7684\u52a0\u5165\uff08\u5982\u9876\u90e8\u6240\u8ff0\uff09<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">transcripts.fasta.transdecoder.pep: \u6700\u7ec8\u5019\u9009ORF\u7684\u80bd\u5e8f\u5217\uff1b\u5220\u9664\u8f83\u957fORF\u4e2d\u7684\u6240\u6709\u8f83\u77ed\u5019\u9009\u9879<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">transcripts.fasta.transdecoder.cds: \u6700\u7ec8\u5019\u9009ORF\u7f16\u7801\u533a\u7684\u6838\u82f7\u9178\u5e8f\u5217<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">transcripts.fasta.transdecoder.gff3: \u6700\u7ec8\u9009\u5b9aORF\u7684\u76ee\u6807\u8f6c\u5f55\u672c\u4e2d\u7684\u4f4d\u7f6e<\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\">transcripts.fasta.transdecoder.bed: \u63cf\u8ff0ORF\u4f4d\u7f6e\u7684bed\u683c\u5f0f\u6587\u4ef6<\/p>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;box-sizing: border-box;\" powered-by=\"xiumi.us\"><br style=\"box-sizing: border-box;\" \/><\/p>\n<p style=\"white-space: normal;box-sizing: border-box;\" powered-by=\"xiumi.us\">\u547d\u4ee4\u662f\u6bd4\u8f83\u7b80\u5355\u7684\uff0c\u5c0f\u4f19\u4f34\u4eec\u53ef\u4ee5\u8bd5\u4e00\u4e0b\u54df\uff0c\u597d\u4e86\uff0c\u4eca\u5929\u7684\u5185\u5bb9\u5c31\u662f\u8fd9\u4e9b\u4e86\uff0c\u5c0f\u4f19\u4f34\u4eec\u6709\u4ec0\u4e48\u95ee\u9898\u6b22\u8fce\u6765\u8ba8\u8bba\u5206\u4eab\u554a\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section>\n<section style=\"box-sizing: border-box;font-size: 16px;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-top: 10px;box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: stretch;flex: 0 0 auto;background-color: rgb(226, 91, 91);min-width: 5%;max-width: 100%;height: auto;padding-top: 5px;padding-right: 9px;padding-left: 20px;box-sizing: border-box;\">\n<section style=\"text-align: justify;color: rgb(255, 255, 255);box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;box-sizing: border-box;\"><strong style=\"box-sizing: border-box;\">\u626b\u7801\u5173\u6ce8\u6211\u4eec<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 5%;max-width: 100%;flex: 0 0 auto;height: auto;align-self: stretch;box-sizing: border-box;\">\n<section style=\"box-sizing: border-box;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 38px 0px 0px 19px;border-color: 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