{"id":4990,"date":"2023-03-25T20:12:43","date_gmt":"2023-03-25T12:12:43","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=4990"},"modified":"2023-03-25T20:12:44","modified_gmt":"2023-03-25T12:12:44","slug":"sequel-iie%e6%98%af%e4%bb%80%e4%b9%88%e9%ac%bc%ef%bc%9f%e5%81%9a%e7%94%9f%e4%bf%a1%e7%9a%84%e4%bd%a0%e6%83%b3%e7%9f%a5%e9%81%93%e5%90%97%ef%bc%9f","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=4990","title":{"rendered":"Sequel IIe\u662f\u4ec0\u4e48\u9b3c\uff1f\u505a\u751f\u4fe1\u7684\u4f60\u60f3\u77e5\u9053\u5417\uff1f"},"content":{"rendered":"\n
\u6bd4\u8f83\u9879<\/th>Sequel IIe<\/th>PacBio<\/th>Oxford Nanopore Technologies<\/th><\/tr><\/thead>
\u5e73\u5747\u8bfb\u957f<\/td>\u7ea62-3 kb<\/td>\u66f4\u957f<\/td>\u66f4\u957f<\/td><\/tr>
\u6570\u636e\u8d28\u91cf<\/td>\u9ad8\uff0c\u9519\u8bef\u7387\u7ea60.1%<\/td>\u6570\u636e\u8d28\u91cf\u7a0d\u5dee\uff0c\u4f46\u53ef\u901a\u8fc7\u591a\u6b21\u6d4b\u5e8f\u548c\u6821\u6b63\u8fdb\u884c\u63d0\u9ad8<\/td>\u6570\u636e\u8d28\u91cf\u8f83\u5dee\uff0c\u9700\u8981\u8fdb\u884c\u4fe1\u53f7\u589e\u5f3a\u548c\u6ee4\u6ce2\u7b49\u5904\u7406<\/td><\/tr>
\u4ef7\u683c<\/td>\u8f83\u4f4e<\/td>\u8f83\u9ad8<\/td>\u8f83\u9ad8<\/td><\/tr>
\u4eea\u5668\u4f53\u79ef<\/td>\u8f83\u5c0f<\/td>\u8f83\u5927<\/td>\u8f83\u5c0f<\/td><\/tr>
\u6837\u54c1\u5904\u7406<\/td>\u9700\u8981\u8f83\u9ad8\u8d28\u91cf\u7684DNA\u6837\u54c1<\/td>DNA\/RNA\u6837\u54c1\u5747\u53ef<\/td>DNA\/RNA\u6837\u54c1\u5747\u53ef<\/td><\/tr>
\u8bfb\u5e8f\u901f\u5ea6<\/td>\u8f83\u6162<\/td>\u8f83\u5feb<\/td>\u8f83\u5feb<\/td><\/tr>
\u5e94\u7528\u573a\u666f<\/td>\u57fa\u56e0\u7ec4\u7ec4\u88c5\u3001\u8f6c\u5f55\u7ec4\u5206\u6790\u3001\u57fa\u56e0\u8bc6\u522b<\/td>\u57fa\u56e0\u7ec4\u7ec4\u88c5\u3001\u8f6c\u5f55\u7ec4\u5206\u6790\u3001\u57fa\u56e0\u8bc6\u522b<\/td>\u57fa\u56e0\u7ec4\u7ec4\u88c5\u3001\u8f6c\u5f55\u7ec4\u5206\u6790\u3001\u57fa\u56e0\u8bc6\u522b<\/td><\/tr>
\u5c40\u9650\u6027\u548c\u6311\u6218<\/td>\u9519\u8bef\u7387\u8f83\u4f4e\u4f46\u4ecd\u5b58\u5728\u9519\u8bef\uff0c\u4fe1\u566a\u6bd4\u8f83\u4f4e<\/td>\u6570\u636e\u8d28\u91cf\u7a0d\u5dee\uff0c\u9700\u8981\u591a\u6b21\u6d4b\u5e8f\u548c\u6821\u6b63<\/td>\u6570\u636e\u8d28\u91cf\u8f83\u5dee\uff0c\u9700\u8981\u8fdb\u884c\u4fe1\u53f7\u589e\u5f3a\u548c\u6ee4\u6ce2\u7b49\u5904\u7406<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\u563f\uff01\u5c0f\u4f19\u4f34\u4eec\uff0c\u6700\u8fd1\u6709\u4e00\u4e2a\u65b0\u6280\u672f\uff0c\u662f\u5173\u4e8e\u4e00\u4e2a\u53eb\u505a”Sequel IIe”\u7684\u65b0\u4e00\u4ee3\u6d4b\u5e8f\u6280\u672f\u7684\u3002\u8fd9\u4e2a\u6280\u672f\u662f\u7531Illumina\u516c\u53f8\u63a8\u51fa\u7684\uff0c\u8ddfPacBio\u516c\u53f8\u7684\u957f\u8bfb\u957f\u5ea6\u6d4b\u5e8f\u6280\u672f\u662f\u7ade\u4e89\u5173\u7cfb\u54e6\u3002Sequel IIe\u901a\u8fc7\u5229\u7528Illumina\u7684”bridged amplification”\u6280\u672f\uff0c\u5728\u5355\u4e2aDNA\u6a21\u677f\u4e0a\u751f\u6210\u6210\u5343\u4e0a\u4e07\u4e2a\u7c7b\u4f3cPCR\u6269\u589e\u7684”cluster”\uff0c\u7136\u540e\u5bf9\u6bcf\u4e2acluster\u8fdb\u884c\u5e76\u884c\u6d4b\u5e8f\u3002Sequel IIe\u6bd4\u8d77PacBio\u7684\u6280\u672f\uff0c\u6570\u636e\u8d28\u91cf\u66f4\u9ad8\uff0c\u800c\u4e14\u4ef7\u683c\u66f4\u4f4e\u3002\u8fd9\u4e2a\u6280\u672f\u4ea7\u751f\u7684\u8bfb\u957f\u957f\u5ea6\u4e5f\u6bd4\u8f83\u957f\uff0c\u5e76\u4e14\u9519\u8bef\u7387\u4e5f\u4e0d\u9ad8\u3002\u5927\u5bb6\u901a\u8fc7\u8fd9\u4e2a\u8868\u683c\u6765\u4e86\u89e3\u4e0b\u8fd9\u4e2a\u6280\u672f\u3002<\/p>\n\n\n\n

<\/p>\n\n\n\n

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\u6bd4\u8f83\u9879 Sequel IIe PacBio Oxford Nanopore Technologies \u5e73\u5747\u8bfb\u957f […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/4990"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4990"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/4990\/revisions"}],"predecessor-version":[{"id":4991,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/4990\/revisions\/4991"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4990"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4990"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4990"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}