<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 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15px;\">\u751f\u4fe1\u4ebaR\u8bed\u8a00\u5b66\u4e60\u5fc5\u5907<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u7acb\u523b\u62e5\u6709\u4e00\u4e2aRstudio\u8d26\u53f7<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u5f00\u542f\u5347\u7ea7\u6a21\u5f0f\u5427<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"color: rgb(255, 129, 36);font-size: 12px;letter-spacing: 0.5px;\">\uff0856\u7ebf\u7a0b\uff0c256G\u5185\u5b58\uff0c\u4e2a\u4eba\u5b58\u50a81T\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 29.9652%;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 29.9652% !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;line-height: 0;\">\n<section>\n<section style=\"display: inline-block;vertical-align: middle;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 0;\">\n<section style=\"width: 111px;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 1.75em;text-indent: 0em;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;height: auto;width: 111px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_GBhsibRAgNiaQsH4ichdP9ibEhHMFA-29.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYngnlT6jiavu08Bvp2iblzGf3IkoBD9NGBhsibRAgNiaQsH4ichdP9ibEhHMFA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"400\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section style=\"display: contents;\" powered-by=\"xiumi.us\">\n<section style=\"display: contents;\">\n<section style=\"display: contents;\">\n<section style=\"height: 0px;overflow: hidden;\"><br \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u57fa\u56e0-\u5fae\u5c0fRNA\uff08miRNA\uff09\u7684\u76f8\u5173\u6027\u5206\u6790\uff0c\u53ef\u4ee5\u63a2\u7d22\u57fa\u56e0\u548cmiRNA\u4e4b\u95f4\u7684\u6f5c\u5728\u76f8\u4e92\u4f5c\u7528\u548c\u8c03\u63a7\u5173\u7cfb\u3002\u610f\u4e49\u5728\u4e8e\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u5206\u6790\u57fa\u56e0\u548cmiRNA\u4e4b\u95f4\u7684\u5173\u7cfb\uff0c\u5e76\u627e\u5230\u4e0e\u76ee\u6807\u57fa\u56e0\u76f8\u5173\u7684miRNA\uff0c\u4ee5\u53ca\u5b83\u4eec\u4e4b\u95f4\u7684\u8c03\u63a7\u673a\u5236\u3002\u8fd9\u5bf9\u4e8e\u6df1\u5165\u7406\u89e3\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u3001\u53d1\u73b0\u6f5c\u5728\u7684\u751f\u7269\u6807\u5fd7\u7269\u7814\u7a76\u75be\u75c5\u7684\u53d1\u751f\u673a\u5236\u7b49\u65b9\u9762\u90fd\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>\u4ee3\u7801\u4e2d\u9009\u62e9\u4e86\u4e00\u4e2a\u76ee\u6807\u57fa\u56e0\uff08gene\uff09\u5e76\u5bf9\u5176\u4e0emiRNA\u7684\u8868\u8fbe\u91cf\u8fdb\u884c\u76f8\u5173\u6027\u5206\u6790\u3002\u901a\u8fc7\u8ba1\u7b97\u76f8\u5173\u7cfb\u6570\u548cp\u503c\uff0c\u53ef\u4ee5\u786e\u5b9a\u4e0e\u76ee\u6807\u57fa\u56e0\u8868\u8fbe\u91cf\u663e\u8457\u76f8\u5173\u7684miRNA\u3002<\/strong><strong>\u4e0b\u9762\u662f\u539f\u59cb\u4e0b\u8f7d\u7684\u57fa\u56e0\u8868\u8fbe\u91cf\u6570\u636e\u548cmirna\u7684\u539f\u59cb\u6570\u636e\u3002<\/strong><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong><br \/><\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6596434359805511\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_aVbyXibrCcJtQZfNy3Y2DicLHLO4Rg-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEYUpoovfTh0uJ9NO9Uwiby4M9zUaiaVbyXibrCcJtQZfNy3Y2DicLHLO4Rg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"617\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6331738437001595\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_fRziaJ1jc50TyQiaRdEcsic4uFubrg-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEWpr1Wdgbs03r5wZ3XhDIdr4hE40fRziaJ1jc50TyQiaRdEcsic4uFubrg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"627\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"text-align: center;white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">#if (!requireNamespace(\"BiocManager\", quietly = TRUE))<\/span><\/code><code><span class=\"code-snippet_outer\"># install.packages(\"BiocManager\")<\/span><\/code><code><span class=\"code-snippet_outer\">#BiocManager::install(\"limma\")<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#install.packages(\"ggpubr\")<\/span><\/code><code><span class=\"code-snippet_outer\">#install.packages(\"ggExtra\")<\/span><\/code><code><span class=\"code-snippet_outer\"># \u9996\u5148\uff0c\u68c0\u67e5\u662f\u5426\u5df2\u7ecf\u5b89\u88c5\u4e86\u540d\u4e3a\"BiocManager\"\u7684\u5305\uff0c\u5982\u679c\u6ca1\u6709\u5219\u8fdb\u884c\u5b89\u88c5\u3002\u63a5\u4e0b\u6765\uff0c\u5b89#\u88c5\"limma\"\u3001\"ggpubr\"\u548c\"ggExtra\"\u5305\u3002<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#\u5f15\u7528\u5305<\/span><\/code><code><span class=\"code-snippet_outer\">library(limma)<\/span><\/code><code><span class=\"code-snippet_outer\">library(reshape2)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggExtra)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">corFilter=0.2 #\u76f8\u5173\u7cfb\u6570\u8fc7\u6ee4\u6807\u51c6<\/span><\/code><code><span class=\"code-snippet_outer\">pvalueFilter=0.001 #pvalue\u8fc7\u6ee4\u6807\u51c6<\/span><\/code><code><span class=\"code-snippet_outer\">gene=\"VCAN\" #\u793a\u4f8b\u5c55\u793a\u7684\u57fa\u56e0\u540d\u79f0<\/span><\/code><code><span class=\"code-snippet_outer\">expFile=\"diffGeneExp.txt\" #\u57fa\u56e0\u8868\u8fbe\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\">miExpFile=\"miRNAmatrix.txt\" #miRNA\u8868\u8fbe\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\">mirnaFile=\"miRNA.txt\" #miRNA\u5217\u8868\u6587\u4ef6\uff0c\u4e4b\u524drownames\u63d0\u53d6\u51fa\u7684\u884c\u540d<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.1516129032258065\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_FTt5p5XuhyK7weC6X2wZxmLLqyhxhA-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkE8TmH6UjskGke0k4h7gverd1OFTt5p5XuhyK7weC6X2wZxmLLqyhxhA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"310\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">#\u521d\u59cb\u5316\u4e86\u4e00\u4e9b\u53d8\u91cf\uff0c\u5305\u62ec\u76f8\u5173\u7cfb\u6570\u8fc7\u6ee4\u9608\u503c\uff08corFilter\uff09\u3001p\u503c\u8fc7\u6ee4\u9608\u503c\uff08pvalueFilter\uff09\u3001<\/span><\/code><code><span class=\"code-snippet_outer\">#\u76ee\u6807\u57fa\u56e0\u540d\u79f0\uff08gene\uff09\u3001\u57fa\u56e0\u8868\u8fbe\u6587\u4ef6\u540d\uff08expFile\uff09\u3001miRNA\u8868\u8fbe\u6587\u4ef6\u540d\uff08miExpFile\uff09\u3001#miRNA\u5217\u8868\u6587\u4ef6\u540d\uff08mirnaFile\uff09<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#\u8bfb\u53d6\u57fa\u56e0\u8868\u8fbe\u6587\u4ef6,\u63d0\u53d6\u76ee\u6807\u57fa\u56e0\u7684\u8868\u8fbe\u91cf<\/span><\/code><code><span class=\"code-snippet_outer\">rt=read.table(expFile, header=T, sep=\"t\", check.names=F)<\/span><\/code><code><span class=\"code-snippet_outer\">rt=as.matrix(rt)<\/span><\/code><code><span class=\"code-snippet_outer\">rownames(rt)=rt[,1]<\/span><\/code><code><span class=\"code-snippet_outer\">exp=rt[,2:ncol(rt)]<\/span><\/code><code><span class=\"code-snippet_outer\">dimnames=list(rownames(exp),colnames(exp))<\/span><\/code><code><span class=\"code-snippet_outer\">data=matrix(as.numeric(as.matrix(exp)),nrow=nrow(exp),dimnames=dimnames)<\/span><\/code><code><span class=\"code-snippet_outer\">data=avereps(data)<\/span><\/code><code><span class=\"code-snippet_outer\">data=data[gene,,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\">#\u4ece\u6307\u5b9a\u7684\u6587\u4ef6\uff08expFile\uff09\u4e2d\u8bfb\u53d6\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u3002\u57fa\u56e0\u540d\u79f0\u4f5c\u4e3a\u884c\u540d\uff0c\u63d0\u53d6\u76ee\u6807\u57fa\u56e0\u7684\u8868\u8fbe<\/span><\/code><code><span class=\"code-snippet_outer\">#\u91cf\u6570\u636e\u3002\u5c06\u8868\u8fbe\u91cf\u6570\u636e\u8f6c\u6362\u4e3a\u77e9\u9635\u5f62\u5f0f\uff0c\u5b58\u50a8\u5728\u53d8\u91cf\"data\"\u4e2d<\/span><\/code><code><span class=\"code-snippet_outer\">#\u5220\u6389\u6b63\u5e38\u6837\u54c1<\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(colnames(data),\"\\-\"), \"[\", 4)<\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(group,\"\"), \"[\", 1)<\/span><\/code><code><span class=\"code-snippet_outer\">group=gsub(\"2\", \"1\", group)<\/span><\/code><code><span class=\"code-snippet_outer\">conData=data[,group==1,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\">treatData=data[,group==0,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\">logFC=mean(treatData)-mean(conData)<\/span><\/code><code><span class=\"code-snippet_outer\">geneExp=data[,group==0,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\"># \u4ece\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u4e2d\u5206\u79bb\u51fa\u6b63\u5e38\u6837\u672c\u548c\u80bf\u7624\u6837\u672c\u3002\u8ba1\u7b97\u80bf\u7624\u6837\u672c\u548c\u6b63\u5e38\u6837\u672c\u7684\u5e73\u5747\u8868\u8fbe<\/span><\/code><code><span class=\"code-snippet_outer\">#\u91cf\u4e4b\u5dee\uff08logFC\uff09\u3002\u5c06\u80bf\u7624\u7ec4\u7684\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u5b58\u50a8\u5728\u53d8\u91cf\"geneExp\"\u4e2d\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">#\u8bfb\u53d6miRNA\u8868\u8fbe\u6570\u636e<\/span><\/code><code><span class=\"code-snippet_outer\">rt1=read.table(miExpFile, header=T, sep=\"t\", check.names=F)<\/span><\/code><code><span class=\"code-snippet_outer\">rt1=as.matrix(rt1)<\/span><\/code><code><span class=\"code-snippet_outer\">rownames(rt1)=rt1[,1]<\/span><\/code><code><span class=\"code-snippet_outer\">exp1=rt1[,2:ncol(rt1)]<\/span><\/code><code><span class=\"code-snippet_outer\">dimnames1=list(rownames(exp1),colnames(exp1))<\/span><\/code><code><span class=\"code-snippet_outer\">miRNA=matrix(as.numeric(as.matrix(exp1)), nrow=nrow(exp1), dimnames=dimnames1)<\/span><\/code><code><span class=\"code-snippet_outer\">miRNA=avereps(miRNA)<\/span><\/code><code><span class=\"code-snippet_outer\">miRNA=miRNA[rowMeans(miRNA)>0,]<\/span><\/code><code><span class=\"code-snippet_outer\">#\u4ece\u6307\u5b9a\u7684\u6587\u4ef6\uff08miExpFile\uff09\u4e2d\u8bfb\u53d6miRNA\u8868\u8fbe\u6570\u636e\u3002\u5c06miRNA\u540d\u79f0\u4f5c\u4e3a\u884c\u540d\uff0c\u63d0\u53d6miRNA<\/span><\/code><code><span class=\"code-snippet_outer\">#\u7684\u8868\u8fbe\u91cf\u6570\u636e\u3002\u5c06\u8868\u8fbe\u91cf\u6570\u636e\u8f6c\u6362\u4e3a\u77e9\u9635\u5f62\u5f0f\uff0c\u5b58\u50a8\u5728\u53d8\u91cf\"miRNA\"\u4e2d<\/span><\/code><code><span class=\"code-snippet_outer\">#\u63d0\u53d6\u76ee\u6807miRNA\u8868\u8fbe\u91cf<\/span><\/code><code><span class=\"code-snippet_outer\">miList=read.table(mirnaFile, header=F, sep=\"t\", check.names=F)<\/span><\/code><code><span class=\"code-snippet_outer\">sameMirna=intersect(row.names(miRNA), as.vector(miList[,1]))<\/span><\/code><code><span class=\"code-snippet_outer\">miRNA=miRNA[sameMirna,]<\/span><\/code><code><span class=\"code-snippet_outer\">miRNA=log2(miRNA+1)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#\u6b63\u5e38\u548c\u80bf\u7624\u6837\u54c1\u6570\u76ee<\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(colnames(miRNA),\"\\-\"), \"[\", 4)<\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(group,\"\"), \"[\", 1)<\/span><\/code><code><span class=\"code-snippet_outer\">group=gsub(\"2\", \"1\", group)<\/span><\/code><code><span class=\"code-snippet_outer\">miRNAtumor=miRNA[,group==0]<\/span><\/code><code><span class=\"code-snippet_outer\">conNum=length(group[group==1]) #\u6b63\u5e38\u7ec4\u6837\u54c1\u6570\u76ee<\/span><\/code><code><span class=\"code-snippet_outer\">treatNum=length(group[group==0]) #\u80bf\u7624\u7ec4\u6837\u54c1\u6570\u76ee<\/span><\/code><code><span class=\"code-snippet_outer\">sampleType=c(rep(\"Normal\",conNum), rep(\"Tumor\",treatNum))<\/span><\/code><code><span class=\"code-snippet_outer\">colnames(miRNAtumor)=gsub(\"(.*?)\\-(.*?)\\-(.*?)\\-(.*?)\\-.*\",\"\\1\\-\\2\\-\\3-\\4\",colnames(miRNAtumor))<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#\u6837\u54c1\u53d6\u4ea4\u96c6<\/span><\/code><code><span class=\"code-snippet_outer\">sameSample=intersect(colnames(miRNAtumor), colnames(geneExp))<\/span><\/code><code><span class=\"code-snippet_outer\">miRNAtumor=miRNAtumor[,sameSample,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\">geneExp=geneExp[,sameSample,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\">y=as.numeric(geneExp[gene,])<\/span><\/code><code><span class=\"code-snippet_outer\"># \u4ecemiRNA\u548c\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u4e2d\u83b7\u53d6\u6837\u672c\u7684\u7c7b\u578b\uff08\u6b63\u5e38\/\u80bf\u7624\uff09\u4fe1\u606f\u3002\u83b7\u53d6\u6837\u672c\u7684\u7c7b\u578b\u548c<\/span><\/code><code><span class=\"code-snippet_outer\">#\u6837\u672c\u6570\u76ee\u3002\u786e\u5b9amiRNA\u548c\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u4e2d\u6837\u672c\u7684\u4ea4\u96c6\uff0c\u5373\u5728\u4e24\u4e2a\u6570\u636e\u4e2d\u90fd\u5b58\u5728\u7684\u6837\u672c\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">#\u76f8\u5173\u6027\u68c0\u9a8c<\/span><\/code><code><span class=\"code-snippet_outer\">outTab=data.frame()<\/span><\/code><code><span class=\"code-snippet_outer\">for(i in row.names(miRNAtumor)){<\/span><\/code><code><span class=\"code-snippet_outer\"> if(sd(miRNAtumor[i,])>0.01){<\/span><\/code><code><span class=\"code-snippet_outer\"> #miRNA\u5dee\u5f02\u5206\u6790<\/span><\/code><code><span class=\"code-snippet_outer\"> miLogFC=mean(miRNA[i,((conNum+1):ncol(miRNA))])-mean(miRNA[i,1:conNum])<\/span><\/code><code><span class=\"code-snippet_outer\"> test=wilcox.test(miRNA[i,] ~ sampleType)<\/span><\/code><code><span class=\"code-snippet_outer\"> diffPvalue=test$p.value<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"> #\u76f8\u5173\u6027\u5206\u6790<\/span><\/code><code><span class=\"code-snippet_outer\"> x=as.numeric(miRNAtumor[i,])<\/span><\/code><code><span class=\"code-snippet_outer\"> corT=cor.test(x, y, method=\"spearma\")<\/span><\/code><code><span class=\"code-snippet_outer\"> cor=corT$estimate<\/span><\/code><code><span class=\"code-snippet_outer\"> pvalue=corT$p.value<\/span><\/code><code><span class=\"code-snippet_outer\"> outTab=rbind(outTab,cbind(Gene=gene, miRNA=i, cor, pvalue, logFC=miLogFC, diffPval=diffPvalue))<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"> #\u5bf9\u6ee1\u8db3\u76f8\u5173\u6027\u8fc7\u6ee4\u6761\u4ef6\u7684miRNA\u8fdb\u884c\u53ef\u89c6\u5316<\/span><\/code><code><span class=\"code-snippet_outer\"> if((cor< -corFilter) & (pvalue<pvalueFilter)){<\/span><\/code><code><span class=\"code-snippet_outer\"> #\u7ed8\u5236\u76f8\u5173\u6027\u6563\u70b9\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"> df1=as.data.frame(cbind(x,y))<\/span><\/code><code><span class=\"code-snippet_outer\"> p1=ggplot(df1, aes(x, y)) + xlab(paste0(i, \" expression\")) + ylab(paste0(gene, \" expression\"))+geom_point() + geom_smooth(method=\"lm\",formula = y ~ x) + theme_bw()+stat_cor(method = 'spearman', aes(x =x, y =y))<\/span><\/code><code><span class=\"code-snippet_outer\"> p2=ggMarginal(p1, type=\"density\", xparams=list(fill = \"orange\"), yparams=list(fill = \"blue\"))<\/span><\/code><code><span class=\"code-snippet_outer\"> #\u4fdd\u5b58\u76f8\u5173\u6027\u56fe\u5f62<\/span><\/code><code><span class=\"code-snippet_outer\"> pdf(file=paste0(\"cor.\",i,\".pdf\"), width=5.2, height=5)<\/span><\/code><code><span class=\"code-snippet_outer\"> print(p2)<\/span><\/code><code><span class=\"code-snippet_outer\"> dev.off()<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"> #miRNA\u5dee\u5f02\u7684\u7bb1\u7ebf\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"> rt=data.frame(Expression=miRNA[i,], Type=sampleType)<\/span><\/code><code><span class=\"code-snippet_outer\"> group=levels(factor(rt$Type))<\/span><\/code><code><span class=\"code-snippet_outer\"> rt$Type=factor(rt$Type, levels=group)<\/span><\/code><code><span class=\"code-snippet_outer\"> comp=combn(group,2)<\/span><\/code><code><span class=\"code-snippet_outer\"> my_comparisons=list()<\/span><\/code><code><span class=\"code-snippet_outer\"> for(k in 1:ncol(comp)){my_comparisons[[k]]<-comp[,k]}<\/span><\/code><code><span class=\"code-snippet_outer\"> #\u7ed8\u5236boxplot<\/span><\/code><code><span class=\"code-snippet_outer\"> boxplot=ggboxplot(rt, x=\"Type\", y=\"Expression\", color=\"Type\",<\/span><\/code><code><span class=\"code-snippet_outer\"> xlab=\"\", ylab=paste0(i, \" expression\"),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.title=\"Type\",<\/span><\/code><code><span class=\"code-snippet_outer\"> palette = c(\"blue\",\"red\"),<\/span><\/code><code><span class=\"code-snippet_outer\"> add = \"jitter\")+ <\/span><\/code><code><span class=\"code-snippet_outer\"> stat_compare_means(comparisons = my_comparisons)<\/span><\/code><code><span class=\"code-snippet_outer\"> #\u8f93\u51fa\u7bb1\u7ebf\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"> pdf(file=paste0(\"diff.\",i,\".pdf\"),width=5,height=4.5)<\/span><\/code><code><span class=\"code-snippet_outer\"> print(boxplot)<\/span><\/code><code><span class=\"code-snippet_outer\"> dev.off()<\/span><\/code><code><span class=\"code-snippet_outer\"> }<\/span><\/code><code><span class=\"code-snippet_outer\"> }<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5bf9miRNA\u8868\u8fbe\u6570\u636e\u4e2d\u7684\u6bcf\u4e2amiRNA\u8fdb\u884c\u5faa\u73af\u904d\u5386\uff1a<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5982\u679c\u8be5miRNA\u7684\u8868\u8fbe\u91cf\u7684\u6807\u51c6\u5dee\u5927\u4e8e0.01\uff1a<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u8ba1\u7b97miRNA\u5728\u80bf\u7624\u7ec4\u548c\u6b63\u5e38\u7ec4\u4e4b\u95f4\u7684\u5e73\u5747\u8868\u8fbe\u91cf\u5dee\u5f02\uff08miLogFC\uff09\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u8fdb\u884cmiRNA\u8868\u8fbe\u91cf\u4e0e\u76ee\u6807\u57fa\u56e0\u8868\u8fbe\u91cf\u4e4b\u95f4\u7684\u76f8\u5173\u6027\u5206\u6790\uff0c\u4f7f\u7528\u7684\u662fSpearman\u76f8\u5173\u7cfb\u6570\u548c\u5bf9\u5e94\u7684p\u503c\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5c06\u76f8\u5173\u6027\u7ed3\u679c\u5b58\u50a8\u5728\u6570\u636e\u6846”outTab”\u4e2d\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5bf9\u6ee1\u8db3\u76f8\u5173\u6027\u8fc7\u6ee4\u6761\u4ef6\u7684miRNA\u8fdb\u884c\u53ef\u89c6\u5316\uff0c\u5305\u62ec\u7ed8\u5236\u76f8\u5173\u6027\u6563\u70b9\u56fe\u548c\u5dee\u5f02\u7684\u7bb1\u7ebf\u56fe\uff0c\u5e76\u5c06\u7ed3\u679c\u4fdd\u5b58\u4e3aPDF\u6587\u4ef6\u3002<\/span><\/p>\n<p><strong><span style=\"font-family: \u5b8b\u4f53;font-size: 10.5pt;\"><o:p><\/o:p><\/span><\/strong><\/p>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><span style=\"color: rgb(51, 51, 51);font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><\/span><span style=\"mso-spacerun:"yes";font-family:"Open Sans";mso-fareast-font-family:\u5b8b\u4f53;color:rgb(51,51,51);font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">#\u8f93\u51fa\u76f8\u5173\u6027\u7ed3\u679c<\/span><\/code><code><span class=\"code-snippet_outer\">outTab=outTab[order(as.numeric(as.vector(outTab[,\"cor\"]))),]<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(file=\"net.network.txt\",outTab,sep=\"t\",quote=F,row.names=F)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#\u8f93\u51fa\u76f8\u5173\u6027\u8282\u70b9\u5c5e\u6027<\/span><\/code><code><span class=\"code-snippet_outer\">miNode=data.frame(Node=unique(as.vector(outTab[,\"miRNA\"])), Type=\"miRNA\")<\/span><\/code><code><span class=\"code-snippet_outer\">geneNode=data.frame(Node=unique(as.vector(outTab[,\"Gene\"])), Type=\"Gene\")<\/span><\/code><code><span class=\"code-snippet_outer\">nodeOut=rbind(miNode, geneNode)<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(nodeOut, file=\"net.node.txt\", sep=\"t\", quote=F, row.names=F)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#\u8f93\u51fa\u76f8\u5173\u6027\u663e\u8457\u7684\u7ed3\u679c<\/span><\/code><code><span class=\"code-snippet_outer\">outTab=outTab[((as.numeric(as.vector(outTab[,\"cor\"]))<-corFilter)&(as.numeric(as.vector(outTab[,\"pvalue\"])) < pvalueFilter)),]<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(file=\"cor.sig.txt\",outTab,sep=\"t\",quote=F,row.names=F)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#\u8f93\u51fa\u76f8\u5173\u6027\u663e\u8457miRNA\u7684\u8868\u8fbe\u91cf<\/span><\/code><code><span class=\"code-snippet_outer\">corMirna=unique(as.vector(outTab[,\"miRNA\"]))<\/span><\/code><code><span class=\"code-snippet_outer\">corMirnaExp=miRNA[corMirna,,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\">corMirnaExp=rbind(ID=colnames(corMirnaExp), corMirnaExp)<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(corMirnaExp, file=\"cor.MirnaExp.txt\", sep=\"t\", quote=F, col.names=F)<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u901a\u8fc7\u57fa\u56e0\u548cmiRNA\u4e4b\u95f4\u7684\u76f8\u5173\u6027\u5206\u6790\uff0c\u53ef\u4ee5\u627e\u5230\u57fa\u56e0\u548cmiRNA\u4e4b\u95f4\u7684\u8c03\u63a7\u5173\u7cfb\u3002\u901a\u8fc7\u5206\u6790\u5b83\u4eec\u4e4b\u95f4\u7684\u76f8\u5173\u6027\uff0c\u53ef\u4ee5\u8bc6\u522b\u51fa\u4e0e\u76ee\u6807\u57fa\u56e0\u8868\u8fbe\u5bc6\u5207\u76f8\u5173\u7684miRNA\u3002\u8fd9\u6709\u52a9\u4e8e\u7406\u89e3\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u4e2d\u7684\u8c03\u8282\u6a21\u5f0f\u548c\u673a\u5236\u3002\u53d1\u73b0\u6f5c\u5728\u7684miRNA\u8c03\u63a7\u9776\u70b9\uff1a\u76f8\u5173\u6027\u5206\u6790\u53ef\u4ee5\u5e2e\u52a9\u786e\u5b9amiRNA\u7684\u6f5c\u5728\u9776\u70b9\u57fa\u56e0\u3002\u5982\u679c\u4e00\u4e2amiRNA\u4e0e\u67d0\u4e2a\u57fa\u56e0\u5448\u73b0\u663e\u8457\u7684\u76f8\u5173\u6027\uff0c\u90a3\u4e48\u8be5\u57fa\u56e0\u5f88\u53ef\u80fd\u662f\u8be5miRNA\u7684\u8c03\u63a7\u9776\u70b9\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u4e0b\u9762\u6211\u4eec\u6765\u770b\u770b\u7ed3\u679c\u8f93\u51fa\u6587\u4ef6\u5217\u8868\u548c\u7ed3\u679c\u56fe\u793a\u8303\u3002<\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"display: none;line-height: 0px;\">\u200d<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.1229508196721312\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_lmAKib4e9wIpicAoYXNItPo7hfOEyQ-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkE2CarhxrvicTEviaJIo6OwD1gCTyxlmAKib4e9wIpicAoYXNItPo7hfOEyQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"244\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.0238095238095237\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_j6l9WoicZr9769dWm3U07Eo43jOiaA-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEVab1z0FbVZSkhLU606led9WBcWj6l9WoicZr9769dWm3U07Eo43jOiaA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"546\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9686346863468634\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_icynk5rN9qXib5DBTsGX0uRUNTcnOw-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEb4XjuZ3yH8YKdOKo88Cn1zlSyhicynk5rN9qXib5DBTsGX0uRUNTcnOw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"542\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.4583333333333333\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_pKSrNecyZ14SD7Eev6OQUG6aSTOrxg-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEgIdOKIvJsW4tEXP8aN4c76rIpKSrNecyZ14SD7Eev6OQUG6aSTOrxg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"216\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.909710391822828\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_2XDHPtXeC9aFUibh1yL4qibr6jWncg-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEEDB1kvasDciafQak4Awy5rzxhhK2XDHPtXeC9aFUibh1yL4qibr6jWncg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"587\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.898936170212766\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_ZKzpumIgibE6BhSfBMthAccLTGEH4w-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEsbGW5SOQWgnSEH0N8qZrdbuJQZKzpumIgibE6BhSfBMthAccLTGEH4w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"564\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e0b\u671f\u5c06\u4e3a\u4f60\u5e26\u6765\u66f4\u591aR\u8bed\u8a00\u7684\u9a9a\u64cd\u4f5c\u6280\u5de7\uff0c\u4ee5\u4e0b\u63a8\u8350\u7684\u662f\u4e00\u4e2a\u591a\u529f\u80fd\u7684\u751f\u4fe1\u5e73\u53f0\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"color: rgb(255, 0, 0);background: rgb(255, 255, 255);font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e91\u751f\u4fe1\u5e73\u53f0\u94fe\u63a5\uff1a<\/span><span style=\"color: rgb(255, 0, 0);background: rgb(255, 255, 255);text-decoration: underline;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">http:\/\/www.biocloudservice.com\/home.html\u3002<\/span><span style=\"color: rgb(255, 0, 0);background: rgb(255, 255, 255);font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><span style=\"color: rgb(255, 0, 0);background: rgb(255, 255, 255);\">\u4e91\u751f\u4fe1\u5e73\u53f0\u94fe\u63a5\uff1ahttp:\/\/www.biocloudservice.com\/home.html\u3002<\/span><span style=\"font-family: \u5b8b\u4f53;\"><o:p><\/o:p><\/span><\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><span style=\"color: rgb(217, 33, 66);font-size: 10.5pt;font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u5176\u4ed6\u76f8\u5173\u5206\u6790\u5185\u5bb9\uff0c\u4f8b\u5982\u9884\u6d4b\u80bf\u7624\u6837\u672c\u836f\u7269\u654f\u611f\u6027\u5206\u6790(http:\/\/www.biocloudservice.com\/712\/712.php),\u9884\u6d4b\u67d0\u6837\u672c\u4e9a\u578b\u5bf9\u514d\u75ab\u6cbb\u7597\u7684\u53cd\u5e94(http:\/\/www.biocloudservice.com\/292\/292.php)\uff0c\u5355\u6837\u672c\u5bcc\u96c6\u7b97\u6cd5\u5206\u6790\u514d\u75ab\u6d78\u6da6\u4e30\u5ea6(http:\/\/www.biocloudservice.com\/106\/106.php)\uff0c\u8ba1\u7b9764\u79cd\u514d\u75ab\u7ec6\u80de\u76f8\u5bf9\u542b\u91cf(http:\/\/www.biocloudservice.com\/107\/107.php)\u7b49\u90fd\u53ef\u4ee5\u7528\u672c\u516c\u53f8\u65b0\u5f00\u53d1\u7684\u96f6\u4ee3\u7801\u4e91\u5e73\u53f0\u751f\u4fe1\u5206\u6790\u5c0f\u5de5\u5177\uff0c\u4e00\u952e\u5b8c\u6210\u8be5\u5206\u6790\u5965\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u6765\u5c1d\u8bd5\u5965\uff0c\u7f51\u5740\uff1ahttp:\/\/www.biocloudservice.com\/home.html\u3002<\/span><span style=\"mso-spacerun:"yes";font-family:\u5fae\u8f6f\u96c5\u9ed1;font-size:10.5000pt;mso-font-kerning:1.0000pt;\">\u4eca\u5929\u5c0f\u679c\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\uff0c\u4e0b\u671f\u5728\u89c1\u5965\u3002<\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;font-size: 10.5pt;letter-spacing: 0.034em;\"><\/span><span style=\"mso-spacerun:"yes";font-family:\u5fae\u8f6f\u96c5\u9ed1;font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><\/span><\/p>\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-top: 0.5em;\">\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);\">\n<section style=\"text-align: left;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;\">\n<section style=\"margin-bottom: 8px;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u70b9\u51fb<strong><span style=\"color: rgb(0, 150, 136);\">\u201c\u9605\u8bfb\u539f\u6587\u201d<\/span><\/strong>\u7acb\u523b\u62e5\u6709<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 136);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u2193\u2193\u2193 <\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"https:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247490130&idx=1&sn=1578a7fab283ba24e1ce5cd6087e2d0d&chksm=cf7c8a97f80b038111f1d3df359dbee67a49462b61b5bfed1060dd83c5d7b975f0db40f4626a#rd\" target=\"_blank\" 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10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;line-height: 0;\">\n<section>\n<section style=\"display: inline-block;vertical-align: middle;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 0;\">\n<section style=\"width: 111px;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 1.75em;text-indent: 0em;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;height: auto;width: 111px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_GBhsibRAgNiaQsH4ichdP9ibEhHMFA-29.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYngnlT6jiavu08Bvp2iblzGf3IkoBD9NGBhsibRAgNiaQsH4ichdP9ibEhHMFA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"400\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section style=\"display: contents;\" powered-by=\"xiumi.us\">\n<section style=\"display: contents;\">\n<section style=\"display: contents;\">\n<section style=\"height: 0px;overflow: hidden;\"><br \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u57fa\u56e0-\u5fae\u5c0fRNA\uff08miRNA\uff09\u7684\u76f8\u5173\u6027\u5206\u6790\uff0c\u53ef\u4ee5\u63a2\u7d22\u57fa\u56e0\u548cmiRNA\u4e4b\u95f4\u7684\u6f5c\u5728\u76f8\u4e92\u4f5c\u7528\u548c\u8c03\u63a7\u5173\u7cfb\u3002\u610f\u4e49\u5728\u4e8e\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u5206\u6790\u57fa\u56e0\u548cmiRNA\u4e4b\u95f4\u7684\u5173\u7cfb\uff0c\u5e76\u627e\u5230\u4e0e\u76ee\u6807\u57fa\u56e0\u76f8\u5173\u7684miRNA\uff0c\u4ee5\u53ca\u5b83\u4eec\u4e4b\u95f4\u7684\u8c03\u63a7\u673a\u5236\u3002\u8fd9\u5bf9\u4e8e\u6df1\u5165\u7406\u89e3\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u3001\u53d1\u73b0\u6f5c\u5728\u7684\u751f\u7269\u6807\u5fd7\u7269\u7814\u7a76\u75be\u75c5\u7684\u53d1\u751f\u673a\u5236\u7b49\u65b9\u9762\u90fd\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>\u4ee3\u7801\u4e2d\u9009\u62e9\u4e86\u4e00\u4e2a\u76ee\u6807\u57fa\u56e0\uff08gene\uff09\u5e76\u5bf9\u5176\u4e0emiRNA\u7684\u8868\u8fbe\u91cf\u8fdb\u884c\u76f8\u5173\u6027\u5206\u6790\u3002\u901a\u8fc7\u8ba1\u7b97\u76f8\u5173\u7cfb\u6570\u548cp\u503c\uff0c\u53ef\u4ee5\u786e\u5b9a\u4e0e\u76ee\u6807\u57fa\u56e0\u8868\u8fbe\u91cf\u663e\u8457\u76f8\u5173\u7684miRNA\u3002<\/strong><strong>\u4e0b\u9762\u662f\u539f\u59cb\u4e0b\u8f7d\u7684\u57fa\u56e0\u8868\u8fbe\u91cf\u6570\u636e\u548cmirna\u7684\u539f\u59cb\u6570\u636e\u3002<\/strong><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong><br \/><\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6596434359805511\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_aVbyXibrCcJtQZfNy3Y2DicLHLO4Rg-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEYUpoovfTh0uJ9NO9Uwiby4M9zUaiaVbyXibrCcJtQZfNy3Y2DicLHLO4Rg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"617\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6331738437001595\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_fRziaJ1jc50TyQiaRdEcsic4uFubrg-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEWpr1Wdgbs03r5wZ3XhDIdr4hE40fRziaJ1jc50TyQiaRdEcsic4uFubrg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"627\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"text-align: center;white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">#if (!requireNamespace(\"BiocManager\", quietly = TRUE))<\/span><\/code><code><span class=\"code-snippet_outer\"># install.packages(\"BiocManager\")<\/span><\/code><code><span class=\"code-snippet_outer\">#BiocManager::install(\"limma\")<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#install.packages(\"ggpubr\")<\/span><\/code><code><span class=\"code-snippet_outer\">#install.packages(\"ggExtra\")<\/span><\/code><code><span class=\"code-snippet_outer\"># \u9996\u5148\uff0c\u68c0\u67e5\u662f\u5426\u5df2\u7ecf\u5b89\u88c5\u4e86\u540d\u4e3a\"BiocManager\"\u7684\u5305\uff0c\u5982\u679c\u6ca1\u6709\u5219\u8fdb\u884c\u5b89\u88c5\u3002\u63a5\u4e0b\u6765\uff0c\u5b89#\u88c5\"limma\"\u3001\"ggpubr\"\u548c\"ggExtra\"\u5305\u3002<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#\u5f15\u7528\u5305<\/span><\/code><code><span class=\"code-snippet_outer\">library(limma)<\/span><\/code><code><span class=\"code-snippet_outer\">library(reshape2)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggExtra)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">corFilter=0.2 #\u76f8\u5173\u7cfb\u6570\u8fc7\u6ee4\u6807\u51c6<\/span><\/code><code><span class=\"code-snippet_outer\">pvalueFilter=0.001 #pvalue\u8fc7\u6ee4\u6807\u51c6<\/span><\/code><code><span class=\"code-snippet_outer\">gene=\"VCAN\" #\u793a\u4f8b\u5c55\u793a\u7684\u57fa\u56e0\u540d\u79f0<\/span><\/code><code><span class=\"code-snippet_outer\">expFile=\"diffGeneExp.txt\" #\u57fa\u56e0\u8868\u8fbe\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\">miExpFile=\"miRNAmatrix.txt\" #miRNA\u8868\u8fbe\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\">mirnaFile=\"miRNA.txt\" #miRNA\u5217\u8868\u6587\u4ef6\uff0c\u4e4b\u524drownames\u63d0\u53d6\u51fa\u7684\u884c\u540d<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.1516129032258065\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_FTt5p5XuhyK7weC6X2wZxmLLqyhxhA-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkE8TmH6UjskGke0k4h7gverd1OFTt5p5XuhyK7weC6X2wZxmLLqyhxhA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"310\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">#\u521d\u59cb\u5316\u4e86\u4e00\u4e9b\u53d8\u91cf\uff0c\u5305\u62ec\u76f8\u5173\u7cfb\u6570\u8fc7\u6ee4\u9608\u503c\uff08corFilter\uff09\u3001p\u503c\u8fc7\u6ee4\u9608\u503c\uff08pvalueFilter\uff09\u3001<\/span><\/code><code><span class=\"code-snippet_outer\">#\u76ee\u6807\u57fa\u56e0\u540d\u79f0\uff08gene\uff09\u3001\u57fa\u56e0\u8868\u8fbe\u6587\u4ef6\u540d\uff08expFile\uff09\u3001miRNA\u8868\u8fbe\u6587\u4ef6\u540d\uff08miExpFile\uff09\u3001#miRNA\u5217\u8868\u6587\u4ef6\u540d\uff08mirnaFile\uff09<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#\u8bfb\u53d6\u57fa\u56e0\u8868\u8fbe\u6587\u4ef6,\u63d0\u53d6\u76ee\u6807\u57fa\u56e0\u7684\u8868\u8fbe\u91cf<\/span><\/code><code><span class=\"code-snippet_outer\">rt=read.table(expFile, header=T, sep=\"t\", check.names=F)<\/span><\/code><code><span class=\"code-snippet_outer\">rt=as.matrix(rt)<\/span><\/code><code><span class=\"code-snippet_outer\">rownames(rt)=rt[,1]<\/span><\/code><code><span class=\"code-snippet_outer\">exp=rt[,2:ncol(rt)]<\/span><\/code><code><span class=\"code-snippet_outer\">dimnames=list(rownames(exp),colnames(exp))<\/span><\/code><code><span class=\"code-snippet_outer\">data=matrix(as.numeric(as.matrix(exp)),nrow=nrow(exp),dimnames=dimnames)<\/span><\/code><code><span class=\"code-snippet_outer\">data=avereps(data)<\/span><\/code><code><span class=\"code-snippet_outer\">data=data[gene,,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\">#\u4ece\u6307\u5b9a\u7684\u6587\u4ef6\uff08expFile\uff09\u4e2d\u8bfb\u53d6\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u3002\u57fa\u56e0\u540d\u79f0\u4f5c\u4e3a\u884c\u540d\uff0c\u63d0\u53d6\u76ee\u6807\u57fa\u56e0\u7684\u8868\u8fbe<\/span><\/code><code><span class=\"code-snippet_outer\">#\u91cf\u6570\u636e\u3002\u5c06\u8868\u8fbe\u91cf\u6570\u636e\u8f6c\u6362\u4e3a\u77e9\u9635\u5f62\u5f0f\uff0c\u5b58\u50a8\u5728\u53d8\u91cf\"data\"\u4e2d<\/span><\/code><code><span class=\"code-snippet_outer\">#\u5220\u6389\u6b63\u5e38\u6837\u54c1<\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(colnames(data),\"\\-\"), \"[\", 4)<\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(group,\"\"), \"[\", 1)<\/span><\/code><code><span class=\"code-snippet_outer\">group=gsub(\"2\", \"1\", group)<\/span><\/code><code><span class=\"code-snippet_outer\">conData=data[,group==1,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\">treatData=data[,group==0,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\">logFC=mean(treatData)-mean(conData)<\/span><\/code><code><span class=\"code-snippet_outer\">geneExp=data[,group==0,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\"># \u4ece\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u4e2d\u5206\u79bb\u51fa\u6b63\u5e38\u6837\u672c\u548c\u80bf\u7624\u6837\u672c\u3002\u8ba1\u7b97\u80bf\u7624\u6837\u672c\u548c\u6b63\u5e38\u6837\u672c\u7684\u5e73\u5747\u8868\u8fbe<\/span><\/code><code><span class=\"code-snippet_outer\">#\u91cf\u4e4b\u5dee\uff08logFC\uff09\u3002\u5c06\u80bf\u7624\u7ec4\u7684\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u5b58\u50a8\u5728\u53d8\u91cf\"geneExp\"\u4e2d\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">#\u8bfb\u53d6miRNA\u8868\u8fbe\u6570\u636e<\/span><\/code><code><span class=\"code-snippet_outer\">rt1=read.table(miExpFile, header=T, sep=\"t\", check.names=F)<\/span><\/code><code><span class=\"code-snippet_outer\">rt1=as.matrix(rt1)<\/span><\/code><code><span class=\"code-snippet_outer\">rownames(rt1)=rt1[,1]<\/span><\/code><code><span class=\"code-snippet_outer\">exp1=rt1[,2:ncol(rt1)]<\/span><\/code><code><span class=\"code-snippet_outer\">dimnames1=list(rownames(exp1),colnames(exp1))<\/span><\/code><code><span class=\"code-snippet_outer\">miRNA=matrix(as.numeric(as.matrix(exp1)), nrow=nrow(exp1), dimnames=dimnames1)<\/span><\/code><code><span class=\"code-snippet_outer\">miRNA=avereps(miRNA)<\/span><\/code><code><span class=\"code-snippet_outer\">miRNA=miRNA[rowMeans(miRNA)>0,]<\/span><\/code><code><span class=\"code-snippet_outer\">#\u4ece\u6307\u5b9a\u7684\u6587\u4ef6\uff08miExpFile\uff09\u4e2d\u8bfb\u53d6miRNA\u8868\u8fbe\u6570\u636e\u3002\u5c06miRNA\u540d\u79f0\u4f5c\u4e3a\u884c\u540d\uff0c\u63d0\u53d6miRNA<\/span><\/code><code><span class=\"code-snippet_outer\">#\u7684\u8868\u8fbe\u91cf\u6570\u636e\u3002\u5c06\u8868\u8fbe\u91cf\u6570\u636e\u8f6c\u6362\u4e3a\u77e9\u9635\u5f62\u5f0f\uff0c\u5b58\u50a8\u5728\u53d8\u91cf\"miRNA\"\u4e2d<\/span><\/code><code><span class=\"code-snippet_outer\">#\u63d0\u53d6\u76ee\u6807miRNA\u8868\u8fbe\u91cf<\/span><\/code><code><span class=\"code-snippet_outer\">miList=read.table(mirnaFile, header=F, sep=\"t\", check.names=F)<\/span><\/code><code><span class=\"code-snippet_outer\">sameMirna=intersect(row.names(miRNA), as.vector(miList[,1]))<\/span><\/code><code><span class=\"code-snippet_outer\">miRNA=miRNA[sameMirna,]<\/span><\/code><code><span class=\"code-snippet_outer\">miRNA=log2(miRNA+1)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#\u6b63\u5e38\u548c\u80bf\u7624\u6837\u54c1\u6570\u76ee<\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(colnames(miRNA),\"\\-\"), \"[\", 4)<\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(group,\"\"), \"[\", 1)<\/span><\/code><code><span class=\"code-snippet_outer\">group=gsub(\"2\", \"1\", group)<\/span><\/code><code><span class=\"code-snippet_outer\">miRNAtumor=miRNA[,group==0]<\/span><\/code><code><span class=\"code-snippet_outer\">conNum=length(group[group==1]) #\u6b63\u5e38\u7ec4\u6837\u54c1\u6570\u76ee<\/span><\/code><code><span class=\"code-snippet_outer\">treatNum=length(group[group==0]) #\u80bf\u7624\u7ec4\u6837\u54c1\u6570\u76ee<\/span><\/code><code><span class=\"code-snippet_outer\">sampleType=c(rep(\"Normal\",conNum), rep(\"Tumor\",treatNum))<\/span><\/code><code><span class=\"code-snippet_outer\">colnames(miRNAtumor)=gsub(\"(.*?)\\-(.*?)\\-(.*?)\\-(.*?)\\-.*\",\"\\1\\-\\2\\-\\3-\\4\",colnames(miRNAtumor))<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#\u6837\u54c1\u53d6\u4ea4\u96c6<\/span><\/code><code><span class=\"code-snippet_outer\">sameSample=intersect(colnames(miRNAtumor), colnames(geneExp))<\/span><\/code><code><span class=\"code-snippet_outer\">miRNAtumor=miRNAtumor[,sameSample,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\">geneExp=geneExp[,sameSample,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\">y=as.numeric(geneExp[gene,])<\/span><\/code><code><span class=\"code-snippet_outer\"># \u4ecemiRNA\u548c\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u4e2d\u83b7\u53d6\u6837\u672c\u7684\u7c7b\u578b\uff08\u6b63\u5e38\/\u80bf\u7624\uff09\u4fe1\u606f\u3002\u83b7\u53d6\u6837\u672c\u7684\u7c7b\u578b\u548c<\/span><\/code><code><span class=\"code-snippet_outer\">#\u6837\u672c\u6570\u76ee\u3002\u786e\u5b9amiRNA\u548c\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u4e2d\u6837\u672c\u7684\u4ea4\u96c6\uff0c\u5373\u5728\u4e24\u4e2a\u6570\u636e\u4e2d\u90fd\u5b58\u5728\u7684\u6837\u672c\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">#\u76f8\u5173\u6027\u68c0\u9a8c<\/span><\/code><code><span class=\"code-snippet_outer\">outTab=data.frame()<\/span><\/code><code><span class=\"code-snippet_outer\">for(i in row.names(miRNAtumor)){<\/span><\/code><code><span class=\"code-snippet_outer\"> if(sd(miRNAtumor[i,])>0.01){<\/span><\/code><code><span class=\"code-snippet_outer\"> #miRNA\u5dee\u5f02\u5206\u6790<\/span><\/code><code><span class=\"code-snippet_outer\"> miLogFC=mean(miRNA[i,((conNum+1):ncol(miRNA))])-mean(miRNA[i,1:conNum])<\/span><\/code><code><span class=\"code-snippet_outer\"> test=wilcox.test(miRNA[i,] ~ sampleType)<\/span><\/code><code><span class=\"code-snippet_outer\"> diffPvalue=test$p.value<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"> #\u76f8\u5173\u6027\u5206\u6790<\/span><\/code><code><span class=\"code-snippet_outer\"> x=as.numeric(miRNAtumor[i,])<\/span><\/code><code><span class=\"code-snippet_outer\"> corT=cor.test(x, y, method=\"spearma\")<\/span><\/code><code><span class=\"code-snippet_outer\"> cor=corT$estimate<\/span><\/code><code><span class=\"code-snippet_outer\"> pvalue=corT$p.value<\/span><\/code><code><span class=\"code-snippet_outer\"> outTab=rbind(outTab,cbind(Gene=gene, miRNA=i, cor, pvalue, logFC=miLogFC, diffPval=diffPvalue))<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"> #\u5bf9\u6ee1\u8db3\u76f8\u5173\u6027\u8fc7\u6ee4\u6761\u4ef6\u7684miRNA\u8fdb\u884c\u53ef\u89c6\u5316<\/span><\/code><code><span class=\"code-snippet_outer\"> if((cor< -corFilter) & (pvalue<pvalueFilter)){<\/span><\/code><code><span class=\"code-snippet_outer\"> #\u7ed8\u5236\u76f8\u5173\u6027\u6563\u70b9\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"> df1=as.data.frame(cbind(x,y))<\/span><\/code><code><span class=\"code-snippet_outer\"> p1=ggplot(df1, aes(x, y)) + xlab(paste0(i, \" expression\")) + ylab(paste0(gene, \" expression\"))+geom_point() + geom_smooth(method=\"lm\",formula = y ~ x) + theme_bw()+stat_cor(method = 'spearman', aes(x =x, y =y))<\/span><\/code><code><span class=\"code-snippet_outer\"> p2=ggMarginal(p1, type=\"density\", xparams=list(fill = \"orange\"), yparams=list(fill = \"blue\"))<\/span><\/code><code><span class=\"code-snippet_outer\"> #\u4fdd\u5b58\u76f8\u5173\u6027\u56fe\u5f62<\/span><\/code><code><span class=\"code-snippet_outer\"> pdf(file=paste0(\"cor.\",i,\".pdf\"), width=5.2, height=5)<\/span><\/code><code><span class=\"code-snippet_outer\"> print(p2)<\/span><\/code><code><span class=\"code-snippet_outer\"> dev.off()<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"> #miRNA\u5dee\u5f02\u7684\u7bb1\u7ebf\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"> rt=data.frame(Expression=miRNA[i,], Type=sampleType)<\/span><\/code><code><span class=\"code-snippet_outer\"> group=levels(factor(rt$Type))<\/span><\/code><code><span class=\"code-snippet_outer\"> rt$Type=factor(rt$Type, levels=group)<\/span><\/code><code><span class=\"code-snippet_outer\"> comp=combn(group,2)<\/span><\/code><code><span class=\"code-snippet_outer\"> my_comparisons=list()<\/span><\/code><code><span class=\"code-snippet_outer\"> for(k in 1:ncol(comp)){my_comparisons[[k]]<-comp[,k]}<\/span><\/code><code><span class=\"code-snippet_outer\"> #\u7ed8\u5236boxplot<\/span><\/code><code><span class=\"code-snippet_outer\"> boxplot=ggboxplot(rt, x=\"Type\", y=\"Expression\", color=\"Type\",<\/span><\/code><code><span class=\"code-snippet_outer\"> xlab=\"\", ylab=paste0(i, \" expression\"),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.title=\"Type\",<\/span><\/code><code><span class=\"code-snippet_outer\"> palette = c(\"blue\",\"red\"),<\/span><\/code><code><span class=\"code-snippet_outer\"> add = \"jitter\")+ <\/span><\/code><code><span class=\"code-snippet_outer\"> stat_compare_means(comparisons = my_comparisons)<\/span><\/code><code><span class=\"code-snippet_outer\"> #\u8f93\u51fa\u7bb1\u7ebf\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"> pdf(file=paste0(\"diff.\",i,\".pdf\"),width=5,height=4.5)<\/span><\/code><code><span class=\"code-snippet_outer\"> print(boxplot)<\/span><\/code><code><span class=\"code-snippet_outer\"> dev.off()<\/span><\/code><code><span class=\"code-snippet_outer\"> }<\/span><\/code><code><span class=\"code-snippet_outer\"> }<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5bf9miRNA\u8868\u8fbe\u6570\u636e\u4e2d\u7684\u6bcf\u4e2amiRNA\u8fdb\u884c\u5faa\u73af\u904d\u5386\uff1a<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5982\u679c\u8be5miRNA\u7684\u8868\u8fbe\u91cf\u7684\u6807\u51c6\u5dee\u5927\u4e8e0.01\uff1a<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u8ba1\u7b97miRNA\u5728\u80bf\u7624\u7ec4\u548c\u6b63\u5e38\u7ec4\u4e4b\u95f4\u7684\u5e73\u5747\u8868\u8fbe\u91cf\u5dee\u5f02\uff08miLogFC\uff09\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u8fdb\u884cmiRNA\u8868\u8fbe\u91cf\u4e0e\u76ee\u6807\u57fa\u56e0\u8868\u8fbe\u91cf\u4e4b\u95f4\u7684\u76f8\u5173\u6027\u5206\u6790\uff0c\u4f7f\u7528\u7684\u662fSpearman\u76f8\u5173\u7cfb\u6570\u548c\u5bf9\u5e94\u7684p\u503c\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5c06\u76f8\u5173\u6027\u7ed3\u679c\u5b58\u50a8\u5728\u6570\u636e\u6846”outTab”\u4e2d\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5bf9\u6ee1\u8db3\u76f8\u5173\u6027\u8fc7\u6ee4\u6761\u4ef6\u7684miRNA\u8fdb\u884c\u53ef\u89c6\u5316\uff0c\u5305\u62ec\u7ed8\u5236\u76f8\u5173\u6027\u6563\u70b9\u56fe\u548c\u5dee\u5f02\u7684\u7bb1\u7ebf\u56fe\uff0c\u5e76\u5c06\u7ed3\u679c\u4fdd\u5b58\u4e3aPDF\u6587\u4ef6\u3002<\/span><\/p>\n<p><strong><span style=\"font-family: \u5b8b\u4f53;font-size: 10.5pt;\"><o:p><\/o:p><\/span><\/strong><\/p>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><span style=\"color: rgb(51, 51, 51);font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><\/span><span style=\"mso-spacerun:"yes";font-family:"Open Sans";mso-fareast-font-family:\u5b8b\u4f53;color:rgb(51,51,51);font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">#\u8f93\u51fa\u76f8\u5173\u6027\u7ed3\u679c<\/span><\/code><code><span class=\"code-snippet_outer\">outTab=outTab[order(as.numeric(as.vector(outTab[,\"cor\"]))),]<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(file=\"net.network.txt\",outTab,sep=\"t\",quote=F,row.names=F)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#\u8f93\u51fa\u76f8\u5173\u6027\u8282\u70b9\u5c5e\u6027<\/span><\/code><code><span class=\"code-snippet_outer\">miNode=data.frame(Node=unique(as.vector(outTab[,\"miRNA\"])), Type=\"miRNA\")<\/span><\/code><code><span class=\"code-snippet_outer\">geneNode=data.frame(Node=unique(as.vector(outTab[,\"Gene\"])), Type=\"Gene\")<\/span><\/code><code><span class=\"code-snippet_outer\">nodeOut=rbind(miNode, geneNode)<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(nodeOut, file=\"net.node.txt\", sep=\"t\", quote=F, row.names=F)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#\u8f93\u51fa\u76f8\u5173\u6027\u663e\u8457\u7684\u7ed3\u679c<\/span><\/code><code><span class=\"code-snippet_outer\">outTab=outTab[((as.numeric(as.vector(outTab[,\"cor\"]))<-corFilter)&(as.numeric(as.vector(outTab[,\"pvalue\"])) < pvalueFilter)),]<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(file=\"cor.sig.txt\",outTab,sep=\"t\",quote=F,row.names=F)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#\u8f93\u51fa\u76f8\u5173\u6027\u663e\u8457miRNA\u7684\u8868\u8fbe\u91cf<\/span><\/code><code><span class=\"code-snippet_outer\">corMirna=unique(as.vector(outTab[,\"miRNA\"]))<\/span><\/code><code><span class=\"code-snippet_outer\">corMirnaExp=miRNA[corMirna,,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\">corMirnaExp=rbind(ID=colnames(corMirnaExp), corMirnaExp)<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(corMirnaExp, file=\"cor.MirnaExp.txt\", sep=\"t\", quote=F, col.names=F)<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u901a\u8fc7\u57fa\u56e0\u548cmiRNA\u4e4b\u95f4\u7684\u76f8\u5173\u6027\u5206\u6790\uff0c\u53ef\u4ee5\u627e\u5230\u57fa\u56e0\u548cmiRNA\u4e4b\u95f4\u7684\u8c03\u63a7\u5173\u7cfb\u3002\u901a\u8fc7\u5206\u6790\u5b83\u4eec\u4e4b\u95f4\u7684\u76f8\u5173\u6027\uff0c\u53ef\u4ee5\u8bc6\u522b\u51fa\u4e0e\u76ee\u6807\u57fa\u56e0\u8868\u8fbe\u5bc6\u5207\u76f8\u5173\u7684miRNA\u3002\u8fd9\u6709\u52a9\u4e8e\u7406\u89e3\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u4e2d\u7684\u8c03\u8282\u6a21\u5f0f\u548c\u673a\u5236\u3002\u53d1\u73b0\u6f5c\u5728\u7684miRNA\u8c03\u63a7\u9776\u70b9\uff1a\u76f8\u5173\u6027\u5206\u6790\u53ef\u4ee5\u5e2e\u52a9\u786e\u5b9amiRNA\u7684\u6f5c\u5728\u9776\u70b9\u57fa\u56e0\u3002\u5982\u679c\u4e00\u4e2amiRNA\u4e0e\u67d0\u4e2a\u57fa\u56e0\u5448\u73b0\u663e\u8457\u7684\u76f8\u5173\u6027\uff0c\u90a3\u4e48\u8be5\u57fa\u56e0\u5f88\u53ef\u80fd\u662f\u8be5miRNA\u7684\u8c03\u63a7\u9776\u70b9\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u4e0b\u9762\u6211\u4eec\u6765\u770b\u770b\u7ed3\u679c\u8f93\u51fa\u6587\u4ef6\u5217\u8868\u548c\u7ed3\u679c\u56fe\u793a\u8303\u3002<\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"display: none;line-height: 0px;\">\u200d<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.1229508196721312\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_lmAKib4e9wIpicAoYXNItPo7hfOEyQ-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkE2CarhxrvicTEviaJIo6OwD1gCTyxlmAKib4e9wIpicAoYXNItPo7hfOEyQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"244\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.0238095238095237\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_j6l9WoicZr9769dWm3U07Eo43jOiaA-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEVab1z0FbVZSkhLU606led9WBcWj6l9WoicZr9769dWm3U07Eo43jOiaA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"546\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9686346863468634\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_icynk5rN9qXib5DBTsGX0uRUNTcnOw-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEb4XjuZ3yH8YKdOKo88Cn1zlSyhicynk5rN9qXib5DBTsGX0uRUNTcnOw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"542\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.4583333333333333\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_pKSrNecyZ14SD7Eev6OQUG6aSTOrxg-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEgIdOKIvJsW4tEXP8aN4c76rIpKSrNecyZ14SD7Eev6OQUG6aSTOrxg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"216\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.909710391822828\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_2XDHPtXeC9aFUibh1yL4qibr6jWncg-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEEDB1kvasDciafQak4Awy5rzxhhK2XDHPtXeC9aFUibh1yL4qibr6jWncg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"587\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.898936170212766\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_ZKzpumIgibE6BhSfBMthAccLTGEH4w-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEsbGW5SOQWgnSEH0N8qZrdbuJQZKzpumIgibE6BhSfBMthAccLTGEH4w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"564\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p><span 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