{"id":50160,"date":"2024-06-16T09:49:41","date_gmt":"2024-06-16T01:49:41","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=50160"},"modified":"2024-06-16T09:49:41","modified_gmt":"2024-06-16T01:49:41","slug":"cibersort%e5%8c%85%ef%bc%9a%e6%b4%9e%e6%82%89%e7%bb%86%e8%83%9e%e5%85%8d%e7%96%ab%e6%b5%b8%e6%b6%a6%e7%9a%84%e7%a5%9e%e5%a5%87%e5%b7%a5%e5%85%b7%ef%bc%81-2","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=50160","title":{"rendered":"CIBERSORT\u5305\uff1a\u6d1e\u6089\u7ec6\u80de\u514d\u75ab\u6d78\u6da6\u7684\u795e\u5947\u5de5\u5177\uff01"},"content":{"rendered":"


\n
\n<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 60px}.rich_media_area_extra{padding:0 60px 21px 60px}}@media (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner,body{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media{padding:20px;overflow:hidden;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}@media screen and (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media_content{overflow:hidden;color:#333;font-size:17px;word-wrap:break-word;-webkit-hyphens:auto;-ms-hyphens:auto;hyphens:auto;text-align:justify;position:relative;z-index:0}.rich_media_content *{max-width:100%!important;box-sizing:border-box!important;-webkit-box-sizing:border-box!important;word-wrap:break-word!important}.rich_media_content p{clear:both;min-height:1em}.rich_media_content em{font-style:italic}.rich_media_content fieldset{min-width:0}.rich_media_content .list-paddingleft-1,.rich_media_content .list-paddingleft-2,.rich_media_content .list-paddingleft-3{padding-left:2.2em}.rich_media_content .list-paddingleft-1 .list-paddingleft-2,.rich_media_content .list-paddingleft-2 .list-paddingleft-2,.rich_media_content .list-paddingleft-3 .list-paddingleft-2{padding-left:30px}.rich_media_content .list-paddingleft-1{padding-left:1.2em}.rich_media_content .list-paddingleft-3{padding-left:3.2em}.rich_media_content .code-snippet,.rich_media_content .code-snippet__fix{max-width:1000%!important}.rich_media_content .code-snippet *,.rich_media_content .code-snippet__fix *{max-width:1000%!important}.rich_media_title{font-size:22px;line-height:1.4;margin-bottom:13px;padding-bottom:13px;border-bottom:1px solid #e7e7eb;}@supports(-webkit-overflow-scrolling:touch){.rich_media_title{font-weight:700}}.rich_media_meta{display:inline-block;vertical-align:middle;padding:0 0 10px 0;font-size:15px;-webkit-tap-highlight-color:rgba(0,0,0,0)}.rich_media_meta.icon_appmsg_tag{margin-right:0px}.rich_media_meta.meta_tag_text{margin-right:0}.rich_media_meta_list em{font-style:normal}.rich_media_meta_text{color:#a5a5a5;}p{margin:0;}.msgBox{margin-top:20px;padding-top:20px;padding-left:50px;overflow:hidden;border-top:2px dashed #09a2ff;}.msg{padding-top:7px;clear:both;}.msgBody{float:right;width:100%;margin-left:55px;padding-bottom:15px;border-bottom:1px dashed #e0e0e0;}.userHeadImg{float:left;margin-left:-50px;}.userHeadImg img{width:40px;height:40px;margin-right:10px;border-radius:3px;}.userName{color:#888888;line-height:24px;font-size:14px;margin:5px 0 5px 0;height:24px;}.replyBody,.autherBody{color:#565656;font-size:15px;}.replyIcon{border-left:4px solid #33ab01;margin-right:5px;}.ad{text-decoration:none;color:#d6d4d4;font-size:12px;}.msgBodyReply{padding-top:5px;}.userName span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation Mono\",Menlo,Courier,monospace}.code-snippet__comment,.code-snippet__quote{color:#afafaf;font-style:italic}.code-snippet__keyword,.code-snippet__selector-tag,.code-snippet__subst{color:#ca7d37}.code-snippet__number,.code-snippet__literal,.code-snippet__variable,.code-snippet__template-variable,.code-snippet__tag .code-snippet__attr{color:#0e9ce5}.code-snippet__string,.code-snippet__doctag{color:#d14}.code-snippet__title,.code-snippet__section,.code-snippet__selector-id{color:#d14}.code-snippet__subst{font-weight:normal}.code-snippet__type,.code-snippet__class .code-snippet__title{color:#0e9ce5}.code-snippet__tag,.code-snippet__name,.code-snippet__attribute{color:#0e9ce5;font-weight:normal}.code-snippet__regexp,.code-snippet__link{color:#ca7d37}.code-snippet__symbol,.code-snippet__bullet{color:#d14}.code-snippet__built_in,.code-snippet__builtin-name{color:#ca7d37}.code-snippet__meta{color:#afafaf}.code-snippet__deletion{background:#fdd}.code-snippet__addition{background:#dfd}.code-snippet__emphasis{font-style:italic}.code-snippet__strong{font-weight:bold}.account_avatar{width:40px;height:40px;padding:0;}.account_info{display:-webkit-box;display:-webkit-flex;display:flex;-webkit-box-align:center;-webkit-align-items:center;padding:20px 0;align-items:center}.flex_bd{padding-left:14px;}.account_nickname{display:inline-block;vertical-align:middle;line-height:1.2;color:#576b95;font-size:14px}.account_desc{overflow:hidden;text-overflow:ellipsis;display:-webkit-box;-webkit-box-orient:vertical;-webkit-line-clamp:1;color:rgba(0,0,0,0.3);font-size:14px;line-height:1.2;padding-top:.4em}.msg_source_url{text-align:left;word-break:break-all;margin-top:20px;}.msg_source_url a{padding-right:10px;}.msg_source_url .url_text{color:#a8a8a8;}.video-desc{font-size:14px;margin-top:15px;color:#6c6c6c;}.msg_source_url{text-align:left;}.original_primary_card_tips{color:rgba(0,0,0,0.3);line-height:1.4;font-size:15px;}.weui-flex__item{margin-bottom:20px;padding:20px 16px;margin-top:16px;line-height:1.4;align-items:center;background-color:#f7f7f7;border-radius:8px;position:relative;}.original_primary_desc{color:rgba(0,0,0,0.5);font-size:14px;padding-top:4px;width:auto;overflow:hidden;text-overflow:ellipsis;}.msgBodyReplyList{border-top:1px solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"CIBERSORT\u5305\uff1a\u6d1e\u6089\u7ec6\u80de\u514d\u75ab\u6d78\u6da6\u7684\u795e\u5947\u5de5\u5177\uff01\",\"time\":\"2023-10-04 19:01:02\",\"timeStamp\":\"1696417262\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247493716&idx=1&sn=11bf9b062d8f143ab1cfc008bad6805e&chksm=cf7f7891f808f187a6298148b7264b1a17c552f068d77caf840c2f69eda04e23ac366b14685b#rd\" target=\"_blank\" rel=\"noopener\">CIBERSORT\u5305\uff1a\u6d1e\u6089\u7ec6\u80de\u514d\u75ab\u6d78\u6da6\u7684\u795e\u5947\u5de5\u5177\uff01<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span id=\"copyright_logo\" class=\"wx_tap_link js_wx_tap_highlight rich_media_meta icon_appmsg_tag appmsg_title_tag weui-wa-hotarea\">\u539f\u521b  <\/span><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u679c  <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c  <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-10-04 19:01:02<\/em><\/div>\n<p><content><\/p>\n<div class=\"topic\">\n<p>\u6536\u5f55\u4e8e\u8bdd\u9898<\/p>\n<p><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2812010014583390210#wechat_redirect\" title=\"R\u4ee3\u7801\" target=\"_blank\" rel=\"noopener\">#R\u4ee3\u7801<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=3142644752182673414#wechat_redirect\" title=\"\u514d\u75ab\u6d78\u6da6\" target=\"_blank\" rel=\"noopener\">#\u514d\u75ab\u6d78\u6da6<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2821072943068839938#wechat_redirect\" title=\"\u751f\u4fe1\u7ed8\u56fe\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u7ed8\u56fe<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=3138206666342268940#wechat_redirect\" title=\"\u751f\u4fe1\u5c0f\u767d\u76f8\u5173\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u5c0f\u767d\u76f8\u5173<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2664545260517294084#wechat_redirect\" title=\"\u751f\u4fe1\u5b9e\u64cd\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u5b9e\u64cd<\/a><\/p>\n<\/div>\n<section data-mpa-powered-by=\"yiban.io\">\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section powered-by=\"xiumi.us\">\n<section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;text-align: left;letter-spacing: 0.034em;\">\u514d\u75ab\u7ec6\u80de\u662f\u6211\u4eec\u8eab\u4f53\u7684\u62a4\u536b\u519b\uff0c\u5b83\u4eec\u5728\u4f53\u5185\u5de1\u6e38\uff0c\u5bfb\u627e\u5e76\u6e05\u9664\u75c5\u539f\u4f53\uff0c\u4fdd\u62a4\u6211\u4eec\u514d\u53d7\u611f\u67d3\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;text-align: left;letter-spacing: 0.034em;\">\u5f53\u6211\u4eec\u611f\u67d3\u6216\u51fa\u73b0\u5176\u4ed6\u5f02\u5e38\u60c5\u51b5\u65f6\uff0c\u514d\u75ab\u7ec6\u80de\u4f1a\u88ab\u5f15\u5bfc\u5230\u76f8\u5e94\u7684\u7ec4\u7ec7\u4e2d\uff0c\u5f62\u6210\u514d\u75ab\u6d78\u6da6\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;text-align: left;letter-spacing: 0.034em;\">\u514d\u75ab\u6d78\u6da6\u662f\u514d\u75ab\u7cfb\u7edf\u54cd\u5e94\u7684\u91cd\u8981\u8868\u73b0\uff0c\u5bf9\u4e8e\u75be\u75c5\u7684\u53d1\u5c55\u548c\u6cbb\u7597\u5177\u6709\u91cd\u8981\u5f71\u54cd\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;text-align: left;letter-spacing: 0.034em;\">\u5728\u80bf\u7624\u4e2d\uff0c\u514d\u75ab\u7ec6\u80de\u7684\u6d78\u6da6\u60c5\u51b5\u5c24\u5176\u91cd\u8981\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;text-align: left;letter-spacing: 0.034em;\">\u4e00\u65b9\u9762\uff0c\u514d\u75ab\u7ec6\u80de\u7684\u6d78\u6da6\u53ef\u4ee5\u5e2e\u52a9\u6e05\u9664\u80bf\u7624\u7ec6\u80de\uff0c\u8d77\u5230\u6297\u80bf\u7624\u4f5c\u7528\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;text-align: left;letter-spacing: 0.034em;\">\u53e6\u4e00\u65b9\u9762\uff0c\u67d0\u4e9b\u80bf\u7624\u53ef\u80fd\u901a\u8fc7\u64cd\u7eb5\u514d\u75ab\u7cfb\u7edf\uff0c\u9003\u907f\u514d\u75ab\u7ec6\u80de\u7684\u653b\u51fb\uff0c\u4ece\u800c\u4fc3\u8fdb\u80bf\u7624\u7684\u751f\u957f\u548c\u8f6c\u79fb\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;text-align: left;letter-spacing: 0.034em;\">\u56e0\u6b64\uff0c\u51c6\u786e\u4e86\u89e3\u514d\u75ab\u7ec6\u80de\u5728\u80bf\u7624\u7ec4\u7ec7\u4e2d\u7684\u5206\u5e03\u548c\u4f5c\u7528\uff0c\u5bf9\u4e8e\u80bf\u7624\u6cbb\u7597\u548c\u9884\u540e\u8bc4\u4f30\u975e\u5e38\u91cd\u8981\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">CIBERSORT\u662f\u4e00\u4e2a\u5f3a\u5927\u7684\u751f\u7269\u4fe1\u606f\u5b66\u5de5\u5177\uff0c\u7531\u65af\u5766\u798f\u5927\u5b66\u7684\u7814\u7a76\u56e2\u961f\u5f00\u53d1\u3002\u5b83\u5229\u7528RNA\u6d4b\u5e8f\u6570\u636e\uff0c\u901a\u8fc7\u89e3\u6790\u514d\u75ab\u57fa\u56e0\u8868\u8fbe\u8c31\uff0c\u4f30\u7b97\u4e0d\u540c\u514d\u75ab\u7ec6\u80de\u5728\u6837\u672c\u4e2d\u7684\u6bd4\u4f8b\u3002\u4f7f\u7528CIBERSORT\uff0c\u6211\u4eec\u53ef\u4ee5\u7cbe\u51c6\u5730\u4e86\u89e3\u7ec4\u7ec7\u4e2d\u4e0d\u540c\u7c7b\u578b\u514d\u75ab\u7ec6\u80de\u7684\u5206\u5e03\u60c5\u51b5\uff0c\u6d1e\u6089\u514d\u75ab\u6d78\u6da6\u7684\u5965\u79d8\u3002CIBERSORTx\u6765\u81ea\u5b98\u7f51\uff08https:\/\/cibersortx.stanford.edu\/\uff09\u7684\u5206\u6790\u6d41\u7a0b\u5982\u56fe\u6240\u793a\uff1a<o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 20px;\"><em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong>CIBERSORTx\u5206\u6790\u6d41\u7a0b<\/strong><\/span><\/em><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.8199300699300699\" data-s=\"300,640\" data-type=\"png\" data-w=\"572\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_3qp70RyVZfFfyibEW2lIYdB8vLf2ow-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwEDYcakdMibMcjoQxWlWTxGbPaYFmICjccPgOibdlu78w5icFg3qp70RyVZfFfyibEW2lIYdB8vLf2ow\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5982\u4f55\u4f7f\u7528CIBERSORT\uff1f\u4f7f\u7528CIBERSORT\u975e\u5e38\u7b80\u5355\uff01\u8ba9\u6211\u4e3a\u5927\u5bb6\u5c55\u793a\u4e00\u4e0b\uff1a<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><span style=\"color: rgb(51, 51, 51);letter-spacing: 0.4pt;background: rgb(255, 255, 255);\">\u4ee3\u7801<\/span>\u5177\u4f53\u5305\u62ec\uff1a<\/span><span style=\"font-family: "Times New Roman";font-size: 10.5pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;\"><em><strong>Step1 \u5206\u6790\u60a3\u8005\u548c\u5bf9\u7167\u6837\u672c\u7684\u6d78\u6da6\u7a0b\u5ea6<\/strong><\/em><\/span><strong style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;\"><span style=\"font-family: "Times New Roman";font-size: 10.5pt;\"><o:p><\/o:p><\/span><\/strong><\/p>\n<section style=\"font-size: 16px;text-align: justify;\" powered-by=\"xiumi.us\"><span style=\"mso-spacerun:"yes";font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";\"><o:p><\/o:p><\/span><\/section>\n<h2 style=\"font-size: 16px;text-align: center;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/h2>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\">\u8fd9\u91cc\u7684LM22.txt \u662fCIBERSORT\u5b98\u65b9\u4e0b\u8f7d\u7684\u6587\u4ef6\uff0cCIBERSORT_exp.txt\u662f\u672c\u5730\u51c6\u5907\u7684\u57fa\u56e0\u8868\u8fbe\u6587\u4ef6\uff0csampleName.csv\u662f\u672c\u5730\u51c6\u5907\u7684\u6837\u672c\u4fe1\u606f\u6587\u4ef6\uff0c\u5c0f\u679c\u7ed9\u5927\u5bb6\u9644\u5728\u6700\u540e\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">########\u5206\u6790\u60a3\u8005\u548c\u5bf9\u7167\u6837\u672c\u7684\u6d78\u6da6\u7a0b\u5ea6<\/span><\/code><code><span class=\"code-snippet_outer\">#\u52a0\u8f7d\u5305<\/span><\/code><code><span class=\"code-snippet_outer\">library(dplyr)<\/span><\/code><code><span class=\"code-snippet_outer\">library(tibble)<\/span><\/code><code><span class=\"code-snippet_outer\">library(tidyr)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggsci)<\/span><\/code><code><span class=\"code-snippet_outer\">library(reshape2)<\/span><\/code><code><span class=\"code-snippet_outer\">setwd(\"D:\/wanglab\/life\/ziyuan\/20230718\/\")<\/span><\/code><code><span class=\"code-snippet_outer\"># devtools::install_github(\"Moonerss\/CIBERSORT\")<\/span><\/code><code><span class=\"code-snippet_outer\">library(CIBERSORT)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u8bbe\u7f6e\u5206\u6790\u4f9d\u8d56\u7684\u57fa\u7840\u8868\u8fbe\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\"># \u6bcf\u7c7b\u514d\u75ab\u7ec6\u80de\u7684\u6807\u5fd7\u6027\u57fa\u56e0\u53ca\u5176\u8868\u8fbe<\/span><\/code><code><span class=\"code-snippet_outer\"># \u57fa\u56e0\u540d\u5b57\u4e3aGene symbol<\/span><\/code><code><span class=\"code-snippet_outer\">LM22.file <- read.delim2(\".\/LM22.txt\",header = T)<\/span><\/code><code><span class=\"code-snippet_outer\">LM22.file[,-1] = lapply(LM22.file[,-1], FUN = function(y){as.numeric(y)})<\/span><\/code><code><span class=\"code-snippet_outer\">row.names(LM22.file) <- LM22.file$Gene.symbol<\/span><\/code><code><span class=\"code-snippet_outer\">LM22.file <- LM22.file[,-1]<\/span><\/code><code><span class=\"code-snippet_outer\">LM22.file <- as.matrix(LM22.file)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u52a0\u8f7d\u81ea\u5df1\u7684\u6570\u636e\u7528\u4e8e\u5206\u6790\u8ba1\u7b97\u514d\u75ab\u7ec6\u80de<\/span><\/code><code><span class=\"code-snippet_outer\">load(\"GSE105450_raw.Rdata\")<\/span><\/code><code><span class=\"code-snippet_outer\">#\u52a0\u8f7d\u81ea\u5df1\u7684\u6570\u636e\u7528\u4e8e\u5206\u6790\u8ba1\u7b97\u514d\u75ab\u7ec6\u80de<\/span><\/code><code><span class=\"code-snippet_outer\"># df <- express[,-c(1,2,4)]<\/span><\/code><code><span class=\"code-snippet_outer\"># #colnames(df)[1] <- \"Gene symbol\"<\/span><\/code><code><span class=\"code-snippet_outer\"># rownames(df) <- df$ORF<\/span><\/code><code><span class=\"code-snippet_outer\"># df <- df[,-1]<\/span><\/code><code><span class=\"code-snippet_outer\">df <- as.data.frame(express)<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(df,\"CIBERSORT_exp.txt\",sep = \"t\",col.names = T,quote = F)<\/span><\/code><code><span class=\"code-snippet_outer\">df <- df[row.names(LM22.file),]<\/span><\/code><code><span class=\"code-snippet_outer\">TCGA_TME.results <- CIBERSORT::cibersort(LM22.file ,df, perm = 1000, QN = F)<\/span><\/code><code><span class=\"code-snippet_outer\">#perm\u7f6e\u6362\u6b21\u6570=1000\uff0cQN\u5206\u4f4d\u6570\u5f52\u4e00\u5316=TRUE<\/span><\/code><code><span class=\"code-snippet_outer\"># perm\u7f6e\u6362\u6b21\u6570=1000<\/span><\/code><code><span class=\"code-snippet_outer\">#\u8f93\u51fa\u7ed3\u679c<\/span><\/code><code><span class=\"code-snippet_outer\">write.csv(TCGA_TME.results, \".\/CIBERSORT_Results_fromRcode.csv\")<\/span><\/code><code><span class=\"code-snippet_outer\">results <- TCGA_TME.results<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;\"><em><strong>Step2 \u7bb1\u7ebf\u56fe\u5c55\u793a<\/strong><\/em><\/span><span style=\"font-family: "Times New Roman";font-size: 10.5pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\">#########\u7bb1\u7ebf\u56fe\u5c55\u793a<\/span><\/code><code><span class=\"code-snippet_outer\">library(stringr)<\/span><\/code><code><span class=\"code-snippet_outer\">group <- sampleName$group[match(str_sub(colnames(df),1,10),sampleName$geo_accession)]<\/span><\/code><code><span class=\"code-snippet_outer\">group <- na.omit(group)<\/span><\/code><code><span class=\"code-snippet_outer\">res <- data.frame(results[,1:22])%>%<\/span><\/code><code><span class=\"code-snippet_outer\"> dplyr::mutate(group = group)%>%<\/span><\/code><code><span class=\"code-snippet_outer\"> tibble::rownames_to_column(\"sample\")%>%<\/span><\/code><code><span class=\"code-snippet_outer\"> tidyr::pivot_longer(cols = colnames(.)[2:23],<\/span><\/code><code><span class=\"code-snippet_outer\"> names_to = \"cell.type\",<\/span><\/code><code><span class=\"code-snippet_outer\"> values_to = 'value')<\/span><\/code><code><span class=\"code-snippet_outer\">head(res,n=6)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"font-size: 16px;text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.36486486486486486\" data-s=\"300,640\" data-type=\"png\" data-w=\"518\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_aFwG9U5qz4iaNSwR8f3KGwfk6971FA-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwEDYcakdMibMcjoQxWlWTxGaoCjdwmicg182Y8NHsxaw41uEFiaFwG9U5qz4iaNSwR8f3KGwfk6971FA\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code><span class=\"code-snippet_outer\">pdf(file = \"CIBERSORT_psignif.pdf\")<\/span><\/code><code><span class=\"code-snippet_outer\">ggplot(res,aes(cell.type,value,fill = group)) + <\/span><\/code><code><span class=\"code-snippet_outer\"> geom_boxplot(outlier.shape = 21,color = \"black\",outlier.color = \"black\") + <\/span><\/code><code><span class=\"code-snippet_outer\"> #theme_bw() +<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_fill_manual(values = c(\"#00BFC4\",\"#F8766D\"))+#\u8bbe\u7f6e\u989c\u8272<\/span><\/code><code><span class=\"code-snippet_outer\"> labs(x = \"Cell Type\", y = \"Estimated Proportion\") +<\/span><\/code><code><span class=\"code-snippet_outer\"> theme(legend.position = \"top\") + <\/span><\/code><code><span class=\"code-snippet_outer\"> theme(axis.text.x = element_text(angle=90,vjust = 0.5,size = 14,face = \"italic\",colour = 'black'),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.text.y = element_text(face = \"italic\",size = 14,colour = 'black'))+<\/span><\/code><code><span class=\"code-snippet_outer\"> #scale_fill_nejm()+<\/span><\/code><code><span class=\"code-snippet_outer\"> stat_compare_means(aes(group = group),label = \"p.signif\",size=3,method = \"kruskal.test\")# p.format;p.signif<\/span><\/code><code><span class=\"code-snippet_outer\">dev.off()<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3888888888888889\" data-s=\"300,640\" data-type=\"jpeg\" data-w=\"1080\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_IlmBAWe69EMUPMhNEgrv6NXX85lJxg-4.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAwyrj7kbUR3ULG9ZUbLrLkgibzL8qUpQROfe8ebc3gNSnu1dIlmBAWe69EMUPMhNEgrv6NXX85lJxg\/640?wx_fmt=jpeg'\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong>\u514d\u75ab\u6d78\u6da6\u7ed3\u679c\u7bb1\u7ebf\u56fe\u6bd4\u8f83<\/strong><span style=\"font-family: \u5b8b\u4f53;font-size: 10.5pt;\"><o:p><\/o:p><\/span><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" data-type=\"png\" data-w=\"497\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_eSn05nHOUXKT6eaFXm0zrgxFk7sFyg-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwEDYcakdMibMcjoQxWlWTxG9b9OVRWPicEgu283cGNXj6kqTeSn05nHOUXKT6eaFXm0zrgxFk7sFyg\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;\"><em><strong>Step3 \u70ed\u56fe\u5c55\u793a<\/strong><\/em><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">library(pheatmap)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u9009\u62e9\u5177\u6709\u5dee\u5f02\u6027\u7684\u514d\u75ab\u7ec6\u80de<\/span><\/code><code><span class=\"code-snippet_outer\">deCell <- c(\"B.cells.naive\",\"Dendritic.cells.activated\",\"Macrophages.M0\",\"Macrophages.M2\",<\/span><\/code><code><span class=\"code-snippet_outer\">\"Mast.cells.activated\",\"Monocytes\",\"Neutrophils\",\"NK.cells.activated\",\"NK.cells.resting\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"T.cells.CD4.memory.resting\",\"T.cells.CD4.naive\",\"T.cells.CD8\",\"T.cells.regulatory..Tregs.\")<\/span><\/code><code><span class=\"code-snippet_outer\">TME.results <- TCGA_TME.results<\/span><\/code><code><span class=\"code-snippet_outer\">re <- TME.results[,-(23:25)]<\/span><\/code><code><span class=\"code-snippet_outer\">#\u5c55\u793a\u5dee\u5f02\u7684\u514d\u75ab\u7ec6\u80de\u7c7b\u578b<\/span><\/code><code><span class=\"code-snippet_outer\">re2 <- as.data.frame(t(re[,deCell]))<\/span><\/code><code><span class=\"code-snippet_outer\">Group = group<\/span><\/code><code><span class=\"code-snippet_outer\">table(Group)<\/span><\/code><code><span class=\"code-snippet_outer\">an = data.frame(group = group,row.names = colnames(re2))<\/span><\/code><code><span class=\"code-snippet_outer\">groupcolor=c(\"#00BFC4\",\"#F8766D\")<\/span><\/code><code><span class=\"code-snippet_outer\">names(groupcolor)<- c(\"Healthy person\",\"RSV\")<\/span><\/code><code><span class=\"code-snippet_outer\">pdf(file=\"\u5dee\u5f02\u514d\u75ab\u7ec6\u80de\u4e30\u5ea6\u70ed\u56fe.pdf\")<\/span><\/code><code><span class=\"code-snippet_outer\">pheatmap::pheatmap(re2,scale = \"row\",<\/span><\/code><code><span class=\"code-snippet_outer\"> show_colnames = F,<\/span><\/code><code><span class=\"code-snippet_outer\"> cluster_rows = F,cluster_cols = F,<\/span><\/code><code><span class=\"code-snippet_outer\"> annotation_col = an,<\/span><\/code><code><span class=\"code-snippet_outer\"> annotation_colors = list(group=groupcolor),<\/span><\/code><code><span class=\"code-snippet_outer\"> color = colorRampPalette(c(\"navy\", \"white\", \"firebrick3\"))(50))<\/span><\/code><code><span class=\"code-snippet_outer\">dev.off()<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><span style=\"font-family: \u5b8b\u4f53;font-size: 10.5pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong>\u5dee\u5f02\u514d\u75ab\u7ec6\u80de\u4e30\u5ea6\u70ed\u56fe<\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" data-type=\"png\" data-w=\"437\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_aRtpJx0Qg4Uyoq7tSCdOciblG8o4Zw-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwEDYcakdMibMcjoQxWlWTxGMIFgYZQ0WpqbvAUzk8NpJcs4DaRtpJx0Qg4Uyoq7tSCdOciblG8o4Zw\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><strong><span style=\"font-family: \u5b8b\u4f53;font-size: 10.5pt;\"><o:p><\/o:p><\/span><\/strong><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-size: 14px;\">\u5e0c\u671b\u901a\u8fc7\u8fd9\u7bc7\u6587\u7ae0\uff0c\u4f60\u80fd\u5bf9<\/span>CIBERSORT<span style=\"font-size: 14px;\">\u6709\u66f4\u6df1\u5165\u7684\u4e86\u89e3\uff0c\u5e76\u5728\u751f\u7269\u4fe1\u606f\u5b66\u7684\u4e16\u754c\u91cc\u63a2\u7d22\u66f4\u591a\u65b0\u5947\u7684\u53ef\u80fd\u6027\u3002\u5982\u679c\u4f60\u5bf9\u7ec6\u80de\u7ec4\u6210\u7684\u7814\u7a76\u5145\u6ee1\u70ed\u60c5\uff0c\u90a3\u5c31\u8d76\u5feb\u52a8\u624b\u5c1d\u8bd5\u4e00\u4e0b<\/span><span style=\"font-size: 14px;\">CIBERSORT<\/span><span style=\"font-size: 14px;\">\u5427\uff01<\/span><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><\/span><\/p>\n<p style=\"letter-spacing: 0.578px;white-space: normal;\"><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u597d\u4e86\u5c0f\u679c\u4eca\u5929\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\u4e86\uff0c<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;color: rgb(0, 0, 0);\">\u5c0f<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;color: rgb(0, 0, 0);\">\u4f19\u4f34\u4eec\uff0c\u4eca\u5929\u6709\u6ca1\u6709\u5b66\u5230\u65b0\u77e5\u8bc6\u5462\uff0c\u60f3\u8981\u7ee7\u7eed\u4e86\u89e3R\u8bed\u8a00\u5185\u5bb9\u53ef\u4ee5\u6301\u7eed\u5173\u6ce8\u5c0f\u679c\u54e6\uff5e\u6216\u8005\u4e5f\u53ef\u4ee5\u5173\u6ce8\u6211\u4eec\u7684\u5b98\u7f51\u4e5f\u4f1a\u6301\u7eed\u66f4\u65b0\u7684\u54e6\uff5e<\/span><strong style=\"letter-spacing: 0.578px;font-size: var(--articleFontsize);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;color: rgb(0, 0, 0);\"><\/span><\/strong><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;\">\n<section style=\"font-size: 16px;\">\n<section powered-by=\"xiumi.us\" style=\"margin: 10px;text-align: left;justify-content: flex-start;display: flex;flex-flow: row;\">\n<section style=\"padding-right: 10px;padding-left: 10px;display: inline-block;width: 558px;vertical-align: top;border-style: dashed;border-width: 1px;border-color: rgb(62, 62, 62);align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;width: 536px;border-width: 0px 0px 0px 10px;border-style: solid;border-left-color: rgb(144, 215, 236);border-right-color: rgb(46, 172, 109);box-shadow: rgb(0, 0, 0) 0px 0px 0px;border-bottom-left-radius: 0px;\">\n<section powered-by=\"xiumi.us\" style=\"padding-right: 3px;padding-left: 3px;\">\n<section style=\"margin-top: 0.95em;margin-bottom: -0.65em;display: inline-block;width: 520px;vertical-align: top;\">\n<section style=\"width: 520px;\">\n<section style=\"border-top: 1px solid rgb(0, 0, 0);width: 519.479px;float: left;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"margin-top: -3px;width: 6px;height: 6px;border-radius: 100%;float: right;background-color: rgb(46, 172, 109);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: -1em;padding: 3px 10px;display: inline-block;vertical-align: top;background-color: rgb(255, 255, 255);color: rgb(0, 154, 68);\">\n<p><strong>\u5f80\u671f\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: justify;\">\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491794&idx=1&sn=2b7a4de95169c79202e44813885a8250&chksm=cf7f7017f808f9015689839fe4057fd418bf7b20eb1dfb5a28cd92e56a41b5605e62bfbc16fb&scene=21#wechat_redirect\" textvalue=\"1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4<\/a><br \/><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491705&idx=1&sn=9ff9b6da28b3a256a99c6697d589be66&chksm=cf7f70bcf808f9aafd1019a0bba3dd3a55e2867dbb9a09203f13c2683adf867094637c405ed1&scene=21#wechat_redirect\" textvalue=\"2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86<\/a><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491909&idx=1&sn=902e34bcf53e605fba2b457ec6a33e36&chksm=cf7f7180f808f8961542f458a49da14e85d8831449fb65fbf2623d55affc564686a5422f56fd&scene=21#wechat_redirect\" textvalue=\"3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8<\/a><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491905&idx=1&sn=fb34ebd6282962c7803badaee7a39096&chksm=cf7f7184f808f8929608c0050344ad86193d0fc6455c1c18e16cc17bf4b37832b63d3b18284e&scene=21#wechat_redirect\" textvalue=\"4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b<\/a><\/section>\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-align: center;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247490130&idx=1&sn=1578a7fab283ba24e1ce5cd6087e2d0d&chksm=cf7c8a97f80b038111f1d3df359dbee67a49462b61b5bfed1060dd83c5d7b975f0db40f4626a&scene=21#wechat_redirect\" textvalue=\"\u4f60\u5df2\u9009\u4e2d\u4e86\u6dfb\u52a0\u94fe\u63a5\u7684\u5185\u5bb9\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.4255555555555556\" data-s=\"300,640\" data-type=\"jpeg\" data-w=\"900\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_3enDMf2LD6pjia3zQolKXIfTwuh6AA-4.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxweqRKIWbRUBSWSGID9aog3q3QVgsHZplb1JyboXh8CPz6b3enDMf2LD6pjia3zQolKXIfTwuh6AA\/640?wx_fmt=jpeg'\" data-recalc-dims=\"1\" \/><\/span><\/a><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;\">\n<section style=\"letter-spacing: 0.578px;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 136);\">\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3888888888888889\" data-s=\"300,640\" data-type=\"jpeg\" data-w=\"1080\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_SibFRUHIk0cCbShYgOsGDPrxeHkEtw-4.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAwyrj7kbUR3ULG9ZUbLrLkgvrEB1jg0GibYczZiaOORjzpxpIjSibFRUHIk0cCbShYgOsGDPrxeHkEtw\/640?wx_fmt=jpeg'\" data-recalc-dims=\"1\" \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>CIBERSORT\u5305\uff1a\u6d1e\u6089\u7ec6\u80de\u514d\u75ab\u6d78\u6da6\u7684\u795e\u5947\u5de5\u5177\uff01 \u539f\u521b  \u5c0f\u679c  \u751f […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/50160"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=50160"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/50160\/revisions"}],"predecessor-version":[{"id":50168,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/50160\/revisions\/50168"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=50160"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=50160"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=50160"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}