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14px;\">\u9996\u5148\u5f53\u7136\u662f\u8f6f\u4ef6\u5b89\u88c5\u73af\u8282\uff0c\u5c0f\u679c\u4f5c\u4e3a\u8d44\u6df1\u7684\u751f\u4fe1\u72d7\uff0c\u4e0d\u8981\u8ddf\u6211\u63d0conda\uff0c\u4ec0\u4e48bioconda\uff0c\u4ec0\u4e48\u4e00\u952e\u5b89\u88c5\u3002\u4e0d\u53ef\u80fd\uff0c\u7edd\u5bf9\u4e0d\u53ef\u80fd\uff0c\u6211\u679c\u5c0f\u679c\u5c31\u662f\u7d2f\u6b7b\uff0c\u6240\u6709\u5934\u53d1\u90fd\u8585\u79c3\uff0c\u4e5f\u4e0d\u4f1a\u7528\u4f60\u4e00\u6b21conda\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;color: rgb(255, 0, 0);\">\u54b3\u54b3\uff0c\u9996\u5148\u8ba9\u6211\u4eec\u6253\u5f00conda,\u5b89\u88c5bwa<\/span><span style=\"font-size: 14px;\"><\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">$ conda install -c bioconda bwa<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5946745562130178\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_tS3y0jkQ0RZvvujmLjbR4I8UuXm2XQ-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAzUmbl9AKuU2I0OTXNMqJibib2E4GOo3GGcZNJPC1Vx1AFBtttS3y0jkQ0RZvvujmLjbR4I8UuXm2XQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"338\" style=\"\" data-recalc-dims=\"1\"><span style=\"font-size: 14px;letter-spacing: 0.578px;text-align: justify;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u53c2\u6570\u5982\u56fe\u6240\u793a\uff0c\u5728bwa\u5de5\u4f5c\u6d41\u7a0b\u4e2d\uff0c\u5e8f\u5217\u6bd4\u5bf9\u5206\u4e3a\u4e24\u6b65\uff0c\u9996\u5148\u9700\u8981\u4f7f\u7528bwa index\u5efa\u7acb\u7d22\u5f15\uff0c\u5176\u4e2d-a\u53ef\u4ee5\u6307\u5b9a\u7d22\u5f15\u7684\u7b97\u6cd5\uff0c\u5176\u4e2d\u5305\u62ecbwtsw\u3001is\u548crb2\uff0cis\u9002\u5408\u7528\u4e8e\u53c2\u8003\u57fa\u56e0\u7ec4\u8f83\u5c0f\u7684\u7269\u79cd\uff0c\u5982\u7ec6\u83cc\uff0c\u4eba\u7c7b\u57fa\u56e0\u7ec4\u5219\u9700\u8981\u4f7f\u7528bwtsw\u3002\u4f4d\u4e8e\u4e2d\u95f4\u7684\u57fa\u56e0\u7ec4\u9009\u54ea\u4e2a\u90fd\u884c\u3002<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">$bwa index human_glk_v37.fasta<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4eba\u5bb6\u8bf4\u53ef\u4ee5\u52a0-a\uff0c\u4e5f\u6ca1\u8bf4\u4e00\u5b9a\u8981\u52a0\u5440\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u6784\u5efa\u5b8cindex\u540e\u5c31\u53ef\u4ee5\u8fdb\u884c\u5e8f\u5217\u6bd4\u5bf9\u4e86\uff0cbwa mem\u540c\u65f6\u652f\u6301\u5355\u7aef\u6d4b\u5e8f\u548c\u53cc\u7aef\u6d4b\u5e8f\u5e8f\u5217\u6bd4\u5bf9\u4ee3\u7801\u5982\u4e0b\u6240\u793a\u3002<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">$ bwa mem -R <span class=\"code-snippet__string\">'@RGtID:E1602061tSM:bartLB:library1'<\/span> -t <span class=\"code-snippet__number\">4<\/span> reference.fna<\/span><\/code><code><span class=\"code-snippet_outer\"> reads<span class=\"code-snippet__number\">.1<\/span>.fq.gz reads<span class=\"code-snippet__number\">.2<\/span>.fq.gz >result.sam<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5176\u4e2d-R\u91cc\u7684\u5185\u5bb9\u975e\u5e38\u91cd\u8981\uff0c\u4e0d\u540clane\u3001\u6587\u5e93\u3001\u6837\u672c\u4f9d\u8d56RG\u8fdb\u884c\u533a\u5206\u540e\u7eedgatk\u8fdb\u884csnp calling\u65f6\u4f1a\u68c0\u67e5-R\u662f\u5426\u7f3a\u5931\uff0c-t\u662f\u6307\u8ba1\u7b97\u7684\u7ebf\u7a0b\u6570\uff0creads1\u548creads2\u6307\u53cc\u7aef\u6d4b\u5e8f\u7684\u4e24\u6761reads\uff0c\u5355\u7aef\u6d4b\u5e8f\u4e5f\u53ef\u4ee5\u53ea\u586b\u4e00\u4e2a\uff0c\u8f93\u51fa\u7684result\u652f\u6301sam\u548cbam\u4e24\u79cd\u683c\u5f0f\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u81f3\u6b64\uff0cfastq\u5df2\u7ecf\u8f6c\u5316\u4e3abam\u6587\u4ef6\u4e86\uff0c\u6b32\u77e5\u540e\u7eed\u5206\u6790\u5982\u4f55\u8fdb\u884c\uff0c\u6b22\u8fce\u5173\u6ce8\u5fae\u4fe1\u516c\u4f17\u53f7\u3002<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"578\" data-cropsely1=\"0\" data-cropsely2=\"322\" data-galleryid=\"\" data-ratio=\"0.7193195625759417\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_oRxs73HBHUoxCtAqBPQnq1AXricRvg-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxenwVZiaibuOTpj6MMDUj2LoPdS6tic40bbGQV7QyM6QOQY8EFoRxs73HBHUoxCtAqBPQnq1AXricRvg\/640?wx_fmt=png'\" data-type=\"jpeg\" data-w=\"823\" style=\"width: 287px;height: 206px;\" data-recalc-dims=\"1\" \/><\/p>\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-top: 0.5em;\">\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);\">\n<section style=\"text-align: left;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;\">\n<section style=\"margin-bottom: 8px;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u70b9\u51fb<strong><span style=\"color: rgb(0, 150, 136);\">\u201c\u9605\u8bfb\u539f\u6587\u201d<\/span><\/strong>\u7acb\u523b\u62e5\u6709<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 136);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u2193\u2193\u2193 <\/span><span style=\"letter-spacing: 0.5px;text-indent: 0em;\"><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5e8f\u5217\u6bd4\u5bf9\u91d1\u6807\u51c6BWA\u00b7\u771f\u4fdd\u59c6\u7ea7\u6559\u7a0b \u539f\u521b \u5c0f\u679c \u751f\u4fe1\u679c […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/50294"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=50294"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/50294\/revisions"}],"predecessor-version":[{"id":50298,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/50294\/revisions\/50298"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=50294"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=50294"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=50294"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

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solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"\u5e8f\u5217\u6bd4\u5bf9\u91d1\u6807\u51c6BWA\u00b7\u771f\u4fdd\u59c6\u7ea7\u6559\u7a0b\",\"time\":\"2023-07-25 19:00:14\",\"timeStamp\":\"1690282814\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247490472&idx=1&sn=22bb4900a8343f5b8180118294f807e3&chksm=cf7c8b6df80b027ba38676465a1ca2c55055dd1b4b46e74bed18fb64663c4293d1a717743fce#rd\" target=\"_blank\" rel=\"noopener\">\u5e8f\u5217\u6bd4\u5bf9\u91d1\u6807\u51c6BWA\u00b7\u771f\u4fdd\u59c6\u7ea7\u6559\u7a0b<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span id=\"copyright_logo\" class=\"wx_tap_link js_wx_tap_highlight rich_media_meta icon_appmsg_tag appmsg_title_tag weui-wa-hotarea\">\u539f\u521b <\/span><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u679c <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-07-25 19:00:14<\/em><\/div>\n<p><content><\/p>\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;font-size: 16px;\">\n<section>\n<section>\n<section>\n<section>\n<section>\n<section data-width=\"100%\" style=\"width: 578px;flex: 0 0 100%;opacity: 1;\">\n<section style=\"line-height: 1.8;\">\n<section>\n<section>\n<section style=\"margin-top: 10px;margin-bottom: 20px;display: flex;flex-flow: row nowrap;text-align: center;justify-content: center;\">\n<section style=\"display: inline-block;width: 578px;vertical-align: top;border-style: solid;border-width: 1px;border-color: rgb(67, 67, 67);flex: 0 0 auto;align-self: flex-start;height: auto;box-shadow: rgb(255, 219, 118) 6px 6px 0px;background-color: rgb(255, 255, 255);\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 403.391px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;\">\n<section style=\"margin-top: 10px;\">\n<section style=\"text-align: center;\">\n<section style=\"font-size: 16px;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;vertical-align: top;width: 70.0348%;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 70.0348% !important;\">\n<section style=\"margin-top: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u751f\u4fe1\u4ebaR\u8bed\u8a00\u5b66\u4e60\u5fc5\u5907<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u7acb\u523b\u62e5\u6709\u4e00\u4e2aRstudio\u8d26\u53f7<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u5f00\u542f\u5347\u7ea7\u6a21\u5f0f\u5427<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"color: rgb(255, 129, 36);font-size: 12px;letter-spacing: 0.5px;\">\uff0856\u7ebf\u7a0b\uff0c256G\u5185\u5b58\uff0c\u4e2a\u4eba\u5b58\u50a81T\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 29.9652%;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 29.9652% !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;line-height: 0;\">\n<section>\n<section style=\"display: inline-block;vertical-align: middle;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 0;\">\n<section style=\"width: 111px;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 1.75em;text-indent: 0em;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;height: auto;width: 111px;\"><img decoding=\"async\" data-ratio=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_GBhsibRAgNiaQsH4ichdP9ibEhHMFA-38.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYngnlT6jiavu08Bvp2iblzGf3IkoBD9NGBhsibRAgNiaQsH4ichdP9ibEhHMFA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"400\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section data-role=\"list\">\n<section>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 8px;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"padding-left: 8px;display: inline-block;width: 578px;vertical-align: top;border-left: 3px solid rgb(219, 219, 219);border-bottom-left-radius: 0px;align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"color: rgba(0, 0, 0, 0.5);text-align: justify;\">\n<p><span style=\"letter-spacing: 0.578px;\"><span style=\"font-size: 14px;letter-spacing: 0.578px;\"><span style=\"font-size: 14px;letter-spacing: 0.578px;\">\u968f\u7740illumina\u4e3a\u4e3b\u7684NGS\u6d4b\u5e8f\u6280\u672f\u7684\u6d41\u884c\uff0c\u6d4b\u5e8f\u6210\u672c\u5728\u77ed\u77ed\u5341\u5e74\u5185\u98de\u901f\u4e0b\u964d\uff0c\u73b0\u5728\u6d4b\u5e8f\u7684\u6210\u672c\u4ec5\u4ec5\u662f\u5341\u5e74\u524d\u7684\u96f6\u5934\uff0c\u8d8a\u6765\u8d8a\u591a\u8bfe\u9898\u7ec4\u9009\u62e9\u57fa\u56e0\u7ec4\u5b66\u5f00\u5c55\u7814\u7a76\u3002<\/span><\/span><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section style=\"font-size: 16px;\">\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5176\u4e2d\uff0c<span style=\"font-size: 14px;color: rgb(255, 0, 0);\">\u77ed\u5e8f\u5217\u6bd4\u5bf9\uff08m<\/span><\/span><span style=\"font-size: 14px;color: rgb(255, 0, 0);\">apping<\/span><span style=\"font-size: 14px;\"><span style=\"font-size: 14px;color: rgb(255, 0, 0);\">\uff09<\/span>\u662fNGS\u5206\u6790\u7684\u91cd\u4e2d\u4e4b\u91cd\uff0c\u76ee\u524d\u6d41\u884c\u7684\u5916\u663e\u5b50\u3001WGS\u3001GBS\u90fd\u9700\u8981\u7528\u5230\u77ed\u5e8f\u5217\u6bd4\u5bf9\u3002\u5728\u4f17\u591a\u77ed\u5e8f\u5217\u6bd4\u5bf9\u4e2d\uff0cBWA\u548cbowtie2\u662f\u5176\u4e2d\u7684\u4f7c\u4f7c\u8005\uff0c\u968f\u7740\u4e09\u4ee3\u6d4b\u5e8flong read\u7684\u5174\u8d77\uff0cBWA\u4e5f\u53ca\u65f6\u8ddf\u8fdb\u4e86\u7b97\u6cd5\uff0c\u589e\u52a0\u4e86split reads\u548cRNAseq\u7684\u6bd4\u5bf9\uff0c\u8ba9BWA\u9690\u9690\u6709\u6210\u4e3a\u91d1\u6807\u51c6\u7684\u8d8b\u52bf\u3002<\/span><\/p>\n<section class=\"channels_iframe_wrp\"><mp-common-videosnap class=\"js_uneditable custom_select_card channels_live_iframe\" data-pluginname=\"mpvideosnap\" data-headimgurl=\"https:\/\/wx.qlogo.cn\/finderhead\/DX68kVlzcbz8hx9HMoThqGJzT37Slibt5l9fBMMKy3pg\/0\" data-username=\"v2_060000231003b20faec8c7e38019c0d4cc02ec33b077ef3bc1e59b4a7f53fd21ec5cd455628a@finder\" data-nickname=\"\u4e91\u751f\u4fe1\u5b66\u751f\u7269\u4fe1\u606f\u5b66\" data-desc=\"\u5c06\u572807\u670827\u65e5 15:00 \u76f4\u64ad\" data-livewording=\"\u9884\u7ea6\" data-intro=\"\u673a\u5668\u5b66\u4e60\u5165\u95e8\u4e0e\u5b9e\u8df5\uff08\u4e09\uff09\uff1aCatboost\u4e0eGBM\u7b97\u6cd5\u9884\u6d4b\u4e73\u817a\u764c\u6076\u6027\u75c5\u53d8\u5bf9\u6bd4\u5206\u6790\" data-type=\"live\" data-status=\"0\" data-noticeid=\"finderlivenotice-v2_060000231003b20faec8c7e38019c0d4cc02ec33b077ef3bc1e59b4a7f53fd21ec5cd455628a@finder-1690162701490641-1678740077\"><\/mp-common-videosnap><\/section>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u76ee\u524dBWA\u652f\u6301\u4e09\u79cd\u7b97\u6cd5\uff0c\u5206\u522b\u662f<span style=\"font-size: 14px;color: rgb(255, 0, 0);\">bwa-backtrack\uff0cbwa-sw\u548cbwa-mem<\/span>\uff0c\u5176\u4e2dbacktrack\u4e3b\u8981\u7528\u4e8e100bp\u4ee5\u4e0b\u7684reads\uff0c\u76ee\u524d\u8f83\u5c11\u5e94\u7528\u3002sw\u548cmem\u7b97\u6cd5\u90fd\u652f\u6301long read\u548csplit\u6bd4\u5bf9\uff0c\u652f\u630170bp\u52301M\u7684reads\uff0c\u5176\u4e2dmem\u7b97\u6cd5\u66f4\u65b0\u3001\u66f4\u5feb\u3001\u66f4\u5f3a\uff0c\u53ef\u7528\u4e8eillumina\u3001454\u3001sanger\u7b49\u591a\u4e2a\u5e73\u53f0\uff0c\u662f\u5b98\u65b9\u6d41\u7a0b\u63a8\u8350\u7684\u7b97\u6cd5\uff0c\u5f88\u660e\u663e\uff0c\u8ddf\u7740\u5b98\u65b9workflow\u8d70\u5c31\u5bf9\u4e86\u3002\u63a5\u4e0b\u6765\u8ddf\u7740\u5c0f\u679c\u8fdb\u5165\u5b9e\u64cd\u73af\u8282\u5427\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u9996\u5148\u5f53\u7136\u662f\u8f6f\u4ef6\u5b89\u88c5\u73af\u8282\uff0c\u5c0f\u679c\u4f5c\u4e3a\u8d44\u6df1\u7684\u751f\u4fe1\u72d7\uff0c\u4e0d\u8981\u8ddf\u6211\u63d0conda\uff0c\u4ec0\u4e48bioconda\uff0c\u4ec0\u4e48\u4e00\u952e\u5b89\u88c5\u3002\u4e0d\u53ef\u80fd\uff0c\u7edd\u5bf9\u4e0d\u53ef\u80fd\uff0c\u6211\u679c\u5c0f\u679c\u5c31\u662f\u7d2f\u6b7b\uff0c\u6240\u6709\u5934\u53d1\u90fd\u8585\u79c3\uff0c\u4e5f\u4e0d\u4f1a\u7528\u4f60\u4e00\u6b21conda\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;color: rgb(255, 0, 0);\">\u54b3\u54b3\uff0c\u9996\u5148\u8ba9\u6211\u4eec\u6253\u5f00conda,\u5b89\u88c5bwa<\/span><span style=\"font-size: 14px;\"><\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">$ conda install -c bioconda bwa<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5946745562130178\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_tS3y0jkQ0RZvvujmLjbR4I8UuXm2XQ-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAzUmbl9AKuU2I0OTXNMqJibib2E4GOo3GGcZNJPC1Vx1AFBtttS3y0jkQ0RZvvujmLjbR4I8UuXm2XQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"338\" style=\"\" data-recalc-dims=\"1\"><span style=\"font-size: 14px;letter-spacing: 0.578px;text-align: justify;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u53c2\u6570\u5982\u56fe\u6240\u793a\uff0c\u5728bwa\u5de5\u4f5c\u6d41\u7a0b\u4e2d\uff0c\u5e8f\u5217\u6bd4\u5bf9\u5206\u4e3a\u4e24\u6b65\uff0c\u9996\u5148\u9700\u8981\u4f7f\u7528bwa index\u5efa\u7acb\u7d22\u5f15\uff0c\u5176\u4e2d-a\u53ef\u4ee5\u6307\u5b9a\u7d22\u5f15\u7684\u7b97\u6cd5\uff0c\u5176\u4e2d\u5305\u62ecbwtsw\u3001is\u548crb2\uff0cis\u9002\u5408\u7528\u4e8e\u53c2\u8003\u57fa\u56e0\u7ec4\u8f83\u5c0f\u7684\u7269\u79cd\uff0c\u5982\u7ec6\u83cc\uff0c\u4eba\u7c7b\u57fa\u56e0\u7ec4\u5219\u9700\u8981\u4f7f\u7528bwtsw\u3002\u4f4d\u4e8e\u4e2d\u95f4\u7684\u57fa\u56e0\u7ec4\u9009\u54ea\u4e2a\u90fd\u884c\u3002<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">$bwa index human_glk_v37.fasta<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4eba\u5bb6\u8bf4\u53ef\u4ee5\u52a0-a\uff0c\u4e5f\u6ca1\u8bf4\u4e00\u5b9a\u8981\u52a0\u5440\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u6784\u5efa\u5b8cindex\u540e\u5c31\u53ef\u4ee5\u8fdb\u884c\u5e8f\u5217\u6bd4\u5bf9\u4e86\uff0cbwa mem\u540c\u65f6\u652f\u6301\u5355\u7aef\u6d4b\u5e8f\u548c\u53cc\u7aef\u6d4b\u5e8f\u5e8f\u5217\u6bd4\u5bf9\u4ee3\u7801\u5982\u4e0b\u6240\u793a\u3002<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">$ bwa mem -R <span class=\"code-snippet__string\">'@RGtID:E1602061tSM:bartLB:library1'<\/span> -t <span class=\"code-snippet__number\">4<\/span> reference.fna<\/span><\/code><code><span class=\"code-snippet_outer\"> reads<span class=\"code-snippet__number\">.1<\/span>.fq.gz reads<span class=\"code-snippet__number\">.2<\/span>.fq.gz >result.sam<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5176\u4e2d-R\u91cc\u7684\u5185\u5bb9\u975e\u5e38\u91cd\u8981\uff0c\u4e0d\u540clane\u3001\u6587\u5e93\u3001\u6837\u672c\u4f9d\u8d56RG\u8fdb\u884c\u533a\u5206\u540e\u7eedgatk\u8fdb\u884csnp calling\u65f6\u4f1a\u68c0\u67e5-R\u662f\u5426\u7f3a\u5931\uff0c-t\u662f\u6307\u8ba1\u7b97\u7684\u7ebf\u7a0b\u6570\uff0creads1\u548creads2\u6307\u53cc\u7aef\u6d4b\u5e8f\u7684\u4e24\u6761reads\uff0c\u5355\u7aef\u6d4b\u5e8f\u4e5f\u53ef\u4ee5\u53ea\u586b\u4e00\u4e2a\uff0c\u8f93\u51fa\u7684result\u652f\u6301sam\u548cbam\u4e24\u79cd\u683c\u5f0f\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> 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