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flex-start;flex: 0 0 auto;height: auto;overflow: hidden;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<h1><\/h1>\n<h1><strong><span style=\"color: rgb(2, 30, 170);\">\u4e00\u5206\u949f\u5b8c\u6210FASTQ\u6587\u4ef6\u8d28\u91cf\u8bc4\u4f30<\/span><\/strong><o:p><\/o:p><\/h1>\n<p>\u4e0a\u4e00\u6b21\u5c0f\u82b1\u7ed9\u5927\u5bb6\u5206\u4eab\u4e86\u5982\u4f55\u5904\u7406FASTQ\u6587\u4ef6\uff0c\u76f8\u4fe1\u806a\u660e\u7684\u5c0f\u4f19\u4f34\u4eec\u5df2\u7ecf\u8f7b\u677e\u638c\u63e1\uff0c\u5bf9\u4e0b\u4e00\u9636\u6bb5\u7684\u8c03\u6574\u8dc3\u8dc3\u6b32\u8bd5\u5566\u3002\u6b63\u5982\u4e0a\u4e00\u6b21\u6240\u8bf4\uff0c\u6570\u636e\u8d28\u91cf\u8bc4\u4f30\u548c\u9884\u5904\u7406\u6b65\u9aa4\u5728\u4f7f\u7528FASTQ\u6587\u4ef6\u8fdb\u884c\u8fdb\u4e00\u6b65\u5206\u6790\u4e4b\u524d\u662f\u5f88\u91cd\u8981\u7684\uff0c\u90a3\u4e48\u8fd9\u4e00\u6b21\u5c0f\u82b1\u5c31\u6765\u7ed9\u5927\u5bb6\u5206\u4eab\u5982\u4f55\u5728\u4e00\u5206\u949f\u5185\u8f7b\u677e\u5b8c\u6210FASTQ\u6587\u4ef6\u8d28\u91cf\u8bc4\u4f30\uff0c\u5e76\u5bf9\u8d28\u91cf\u8bc4\u4f30\u7ed3\u679c\u9644\u4e0a\u8be6\u7ec6\u7684\u8bf4\u660e\uff0c\u5e72\u8d27\u6ee1\u6ee1\uff0c\u5c0f\u4f19\u4f34\u4eec\u5feb\u6765\u770b\u770b\u5427\u3002<o:p><\/o:p><\/p>\n<p><br \/><\/p>\n<h2><span style=\"color: rgb(2, 30, 170);\"><strong>\u4e3a\u4ec0\u4e48\u8981\u8fdb\u884c\u8d28\u91cf\u8bc4\u4f30\u548c\u9884\u5904\u7406\uff1f<\/strong><\/span><o:p><\/o:p><\/h2>\n<p>1.\u9519\u8bef\u8bc6\u522b\u548c\u4fee\u6b63\uff1aFASTQ\u6587\u4ef6\u5305\u542b\u4e86\u6d4b\u5e8f\u5b9e\u9a8c\u751f\u6210\u7684\u539f\u59cb\u78b1\u57fa\u5e8f\u5217\u53ca\u5176\u5bf9\u5e94\u7684\u8d28\u91cf\u503c\u3002\u8fd9\u4e9b\u8d28\u91cf\u503c\u53cd\u6620\u4e86\u6bcf\u4e2a\u78b1\u57fa\u6d4b\u5e8f\u7684\u53ef\u4fe1\u5ea6\u3002\u6570\u636e\u8d28\u91cf\u8bc4\u4f30\u53ef\u4ee5\u5e2e\u52a9\u8bc6\u522b\u548c\u4fee\u6b63\u53ef\u80fd\u5b58\u5728\u7684\u6d4b\u5e8f\u9519\u8bef\uff0c\u4f8b\u5982\u6d4b\u5e8f\u78b1\u57fa\u66ff\u4ee3\u3001\u63d2\u5165\u6216\u5220\u9664\u9519\u8bef\u3002\u901a\u8fc7\u4fee\u590d\u8fd9\u4e9b\u9519\u8bef\uff0c\u53ef\u4ee5\u63d0\u9ad8\u540e\u7eed\u5206\u6790\u7684\u51c6\u786e\u6027\u3002<o:p><\/o:p><\/p>\n<p>2.\u5197\u4f59\u548c\u4f4e\u8d28\u91cf\u7247\u6bb5\u8fc7\u6ee4\uff1aFASTQ\u6587\u4ef6\u901a\u5e38\u5305\u542b\u5927\u91cf\u7684\u6d4b\u5e8f\u7247\u6bb5\uff0c\u5176\u4e2d\u53ef\u80fd\u5b58\u5728\u5197\u4f59\u5e8f\u5217\u3001PCR\u91cd\u590d\u6216\u8005\u4f4e\u8d28\u91cf\u7684\u7247\u6bb5\u3002\u5bf9\u6570\u636e\u8fdb\u884c\u8d28\u91cf\u8bc4\u4f30\u548c\u9884\u5904\u7406\u53ef\u4ee5\u8fc7\u6ee4\u6389\u8fd9\u4e9b\u4e0d\u5fc5\u8981\u7684\u7247\u6bb5\uff0c\u51cf\u5c11\u6570\u636e\u91cf\u548c\u566a\u97f3\uff0c\u63d0\u9ad8\u540e\u7eed\u5206\u6790\u7684\u6548\u7387\u548c\u51c6\u786e\u6027\u3002<o:p><\/o:p><\/p>\n<p>3.\u8d28\u91cf\u63a7\u5236\uff1a\u6570\u636e\u8d28\u91cf\u8bc4\u4f30\u53ef\u4ee5\u63d0\u4f9b\u5173\u4e8e\u6d4b\u5e8f\u6570\u636e\u8d28\u91cf\u7684\u8be6\u7ec6\u7edf\u8ba1\u4fe1\u606f\uff0c\u4f8b\u5982\u6bcf\u4e2a\u78b1\u57fa\u7684\u8d28\u91cf\u5206\u5e03\u3001\u6d4b\u5e8f\u6df1\u5ea6\u7b49\u3002\u8fd9\u4e9b\u4fe1\u606f\u5bf9\u4e8e\u9009\u62e9\u5408\u9002\u7684\u9608\u503c\u3001\u786e\u5b9a\u5206\u6790\u65b9\u6cd5\u548c\u89e3\u91ca\u7ed3\u679c\u975e\u5e38\u91cd\u8981\u3002\u901a\u8fc7\u5bf9\u6570\u636e\u8d28\u91cf\u8fdb\u884c\u63a7\u5236\uff0c\u53ef\u4ee5\u786e\u4fdd\u540e\u7eed\u5206\u6790\u7684\u53ef\u9760\u6027\u548c\u53ef\u91cd\u590d\u6027\u3002<o:p><\/o:p><\/p>\n<p>4.\u6570\u636e\u6e05\u6d17\uff1a\u5728\u8fdb\u884c\u67d0\u4e9b\u5206\u6790\u4e4b\u524d\uff0c\u9700\u8981\u5bf9\u6570\u636e\u8fdb\u884c\u6e05\u6d17\uff0c\u4ee5\u53bb\u9664\u4f4e\u8d28\u91cf\u7684\u78b1\u57fa\u6216\u8005\u4e0d\u53ef\u9760\u7684\u7247\u6bb5\u3002\u8fd9\u6837\u53ef\u4ee5\u6392\u9664\u566a\u97f3\u5f15\u5165\u548c\u504f\u5dee\uff0c\u786e\u4fdd\u5206\u6790\u7ed3\u679c\u66f4\u52a0\u51c6\u786e\u548c\u53ef\u9760\u3002<span style=\"mso-bookmark:\u4e3a\u4ec0\u4e48\u8981\u8fdb\u884c\u8d28\u91cf\u8bc4\u4f30\u548c\u9884\u5904\u7406;\"><\/span><\/p>\n<p><span style=\"mso-bookmark:\u4e3a\u4ec0\u4e48\u8981\u8fdb\u884c\u8d28\u91cf\u8bc4\u4f30\u548c\u9884\u5904\u7406;\"><span lang=\"EN-US\"><o:p><\/o:p><\/span><\/span><\/p>\n<h2><strong><span style=\"color: rgb(2, 30, 170);\">FASTQ<\/span><\/strong><strong><span style=\"color: rgb(2, 30, 170);\">\u6570\u636e\u8d28\u63a7\u8fc7\u6ee4\u8f6f\u4ef6\u2014SOAPnuke<\/span><\/strong><\/h2>\n<h2><o:p><\/o:p><\/h2>\n<p>\u5f53\u4eca\u5e02\u9762\u4e0a\u6709\u8bb8\u591a\u53ef\u4ee5\u5bf9FASTQ\u6570\u636e\u8fdb\u884c\u8d28\u63a7\u8fc7\u6ee4\u7684\u8f6f\u4ef6\uff0cSOAPnuke\u662f\u5176\u4e2d\u5bf9\u65b0\u624b\u6bd4\u8f83\u53cb\u597d\uff0c\u4f7f\u7528\u8d77\u6765\u6bd4\u8f83\u7b80\u5355\u7684\u5165\u95e8\u7ea7\u8f6f\u4ef6\u3002\u5c0f\u679c\u8fd9\u6b21\u5c31\u5728linux\u4e2d\u4ee5SOAPnuke\u4e3a\u4f8b\uff0c\u6559\u7ed9\u5927\u5bb6\u8d28\u91cf\u8bc4\u4f30\u7684\u6b65\u9aa4\u3002<o:p><\/o:p><\/p>\n<p>\u4e0b\u9762\u662fSOAPnuke\u7684\u4e00\u4e9b\u5e38\u7528\u53c2\u6570\u7684\u4ecb\u7ecd\uff1a<o:p><\/o:p><\/p>\n<p>1.-1\uff1a\u6307\u5b9a\u5f85\u5904\u7406\u6570\u636e\u7684\u7b2c\u4e00\u5bf9\uff08\u6216\u552f\u4e00\uff09\u6d4b\u5e8f\u6587\u4ef6\u7684\u8def\u5f84\u3002<o:p><\/o:p><\/p>\n<p>2.-2\uff1a\u6307\u5b9a\u5f85\u5904\u7406\u6570\u636e\u7684\u7b2c\u4e8c\u5bf9\u6d4b\u5e8f\u6587\u4ef6\u7684\u8def\u5f84\uff08\u5982\u679c\u662f\u53cc\u7aef\u6d4b\u5e8f\u6570\u636e\uff09\u3002<o:p><\/o:p><\/p>\n<p>3.-r\uff1a\u6307\u5b9a\u4fdd\u5b58\u5904\u7406\u7ed3\u679c\u7684\u8def\u5f84\u3002<o:p><\/o:p><\/p>\n<p>4.-C\uff1a\u7528\u4e8e\u6307\u5b9a\u914d\u7f6e\u6587\u4ef6\u7684\u8def\u5f84\u3002\u914d\u7f6e\u6587\u4ef6\u5305\u542b\u4e86SOAPnuke\u7684\u8be6\u7ec6\u8bbe\u7f6e\uff0c\u5982\u5904\u7406\u8fc7\u7a0b\u4e2d\u6240\u4f7f\u7528\u7684\u8d28\u91cf\u8ba1\u7b97\u65b9\u6cd5\u3001\u8d28\u91cf\u9608\u503c\u7b49\u3002\u53ef\u4ee5\u6839\u636e\u9700\u8981\u8fdb\u884c\u81ea\u5b9a\u4e49\u8bbe\u7f6e\u3002<o:p><\/o:p><\/p>\n<p>5.-5\uff1a\u6307\u5b9a5’\u7aef\u526a\u5207\u7684\u78b1\u57fa\u6570\u3002\u8be5\u53c2\u6570\u7528\u4e8e\u53bb\u9664\u6d4b\u5e8f\u6587\u5e93\u4e2d\u7684\u4f4e\u8d28\u91cf\u672b\u7aef\uff0c\u63d0\u9ad8\u6570\u636e\u8d28\u91cf\u3002<o:p><\/o:p><\/p>\n<p>6.-3\uff1a\u6307\u5b9a3’\u7aef\u526a\u5207\u7684\u78b1\u57fa\u6570\u3002\u8be5\u53c2\u6570\u540c\u6837\u7528\u4e8e\u53bb\u9664\u6d4b\u5e8f\u6587\u5e93\u4e2d\u7684\u4f4e\u8d28\u91cf\u672b\u7aef\u3002<o:p><\/o:p><\/p>\n<p>7.-l\uff1a\u6307\u5b9a\u6700\u77ed\u6709\u6548\u8bfb\u957f\uff08clean read length\uff09\u3002\u8be5\u53c2\u6570\u7528\u4e8e\u53bb\u9664\u8f83\u77ed\u7684reads\uff0c\u63d0\u9ad8\u6570\u636e\u8d28\u91cf\u3002<o:p><\/o:p><\/p>\n<p>8.-q\uff1a\u6307\u5b9a\u8d28\u91cf\u9608\u503c\u3002\u8be5\u53c2\u6570\u7528\u4e8e\u5c06\u4f4e\u4e8e\u6307\u5b9a\u8d28\u91cf\u9608\u503c\u7684\u78b1\u57fa\u6807\u8bb0\u4e3aN\uff0c\u4ece\u800c\u53bb\u9664\u4f4e\u8d28\u91cf\u7684\u78b1\u57fa\u3002<o:p><\/o:p><\/p>\n<p>9.-n\uff1a\u6307\u5b9a\u5141\u8bb8\u7684\u6700\u5927\u8fde\u7eed\u4f4e\u8d28\u91cf\u78b1\u57fa\u6570\u3002\u8be5\u53c2\u6570\u7528\u4e8e\u53bb\u9664\u5177\u6709\u8fde\u7eed\u4f4e\u8d28\u91cf\u78b1\u57fa\u7684reads\u3002<o:p><\/o:p><\/p>\n<p>10.-M\uff1a\u6307\u5b9a\u4fdd\u7559\u7684\u6700\u5c0f\u5e73\u5747\u8d28\u91cf\u503c\u3002\u8be5\u53c2\u6570\u7528\u4e8e\u8fc7\u6ee4\u6389\u5e73\u5747\u8d28\u91cf\u4f4e\u4e8e\u6307\u5b9a\u9608\u503c\u7684reads\u3002<o:p><\/o:p><\/p>\n<p>11.-d\uff1a\u6307\u5b9aadapter\u5e8f\u5217\u6587\u4ef6\u7684\u8def\u5f84\u3002\u8be5\u53c2\u6570\u7528\u4e8e\u53bb\u9664reads\u4e2d\u7684adapter\u5e8f\u5217\u3002<\/p>\n<p>12.-t\uff1a\u6307\u5b9a\u7ebf\u7a0b\u6570\u3002\u8be5\u53c2\u6570\u7528\u4e8e\u6307\u5b9a\u5e76\u884c\u5904\u7406\u7684\u7ebf\u7a0b\u6570\uff0c\u52a0\u5feb\u5904\u7406\u901f\u5ea6\u3002<o:p><\/o:p><\/p>\n<p>13.-D\uff1a\u5f00\u542fadapter\u5e8f\u5217\u7684\u5b58\u5728\u6027\u68c0\u6d4b\u548c\u53bb\u9664\u3002<o:p><\/o:p><\/p>\n<p>14.-I\uff1a\u5f00\u542fPCR\u5197\u4f59\u5e8f\u5217\u7684\u68c0\u6d4b\u548c\u53bb\u9664\u3002<o:p><\/o:p><\/p>\n<p>15.-O\uff1a\u5f00\u542fPCR\u5197\u4f59\u5e8f\u5217\u914d\u5bf9\u6bd4\u5bf9\u7684\u68c0\u6d4b\u548c\u53bb\u9664\u3002<o:p><\/o:p><\/p>\n<p>\u8fd9\u4e9b\u53ea\u662fSOAPnuke\u7684\u4e00\u4e9b\u5e38\u7528\u53c2\u6570\uff0c\u8fd8\u6709\u5176\u4ed6\u4e00\u4e9b\u53ef\u9009\u53c2\u6570\u7528\u4e8e\u66f4\u8be6\u7ec6\u5730\u63a7\u5236\u9884\u5904\u7406\u8fc7\u7a0b\u3002\u4f7f\u7528\u9002\u5f53\u7684\u53c2\u6570\u8bbe\u7f6e\u53ef\u4ee5\u6839\u636e\u5b9e\u9645\u9700\u6c42\u8fdb\u884c\u6570\u636e\u8d28\u91cf\u63a7\u5236\u548c\u9884\u5904\u7406\uff0c\u63d0\u9ad8\u540e\u7eed\u5206\u6790\u7684\u51c6\u786e\u6027\u548c\u53ef\u9760\u6027\u3002\u5c0f\u4f19\u4f34\u4eec\u5982\u679c\u60f3\u4e86\u89e3\u7684\u8bdd\u53ef\u4ee5\u5728\u547d\u4ee4\u884c\u4e2d\u8fd0\u884cSOAPnuke filter \u2013help\uff0c\u67e5\u770bSOAPnuke\u66f4\u591a\u5b8c\u6574\u53c2\u6570\u3002<o:p><\/o:p><\/p>\n<p><br \/><\/p>\n<h2><span style=\"color: rgb(2, 30, 170);\"><strong>\u64cd\u4f5c\u6b65\u9aa4<\/strong><\/span><span lang=\"EN-US\"><o:p><\/o:p><\/span><\/h2>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u521b\u5efa\u6587\u4ef6\u5939<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">mkdir<\/span> <span class=\"code-snippet__number\">00_<\/span><span class=\"code-snippet__keyword\">ref<\/span> <span class=\"code-snippet__number\">01_<\/span>clean <span class=\"code-snippet__number\">02_<\/span>align <span class=\"code-snippet__number\">03_<\/span>SNPCalling <span class=\"code-snippet__number\">04_<\/span>annotation raw_data<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p>\u4e4b\u6240\u4ee5\u521b\u5efa\u8fd9\u4e48\u591a\u6587\u4ef6\u5939\u662f\u56e0\u4e3a\u8d28\u91cf\u8bc4\u4f30\u53ea\u662f\u6211\u4eec\u5206\u6790\u6d41\u7a0b\u7684\u7b2c\u4e00\u6b65\uff0c\u5728\u540e\u7eed\u5206\u6790\u6d41\u7a0b\u8fd8\u4f1a\u4ea7\u751f\u4ee5\u4e0b\u6587\u4ef6\uff1a<o:p><\/o:p><\/p>\n<p>\u8fc7\u6ee4\u2192clean.fq\u2192\u6bd4\u5bf9\u2192clean.bwa.sam\u2192\u6570\u636e\u683c\u5f0f\u8f6c\u6362\u2192clean.bwa.bam\u2192\u7d22\u5f15\u7ed3\u679c\u6392\u5e8f\u2192clean.bwa.sorted.bam\u2192\u53d8\u5f02\u68c0\u6d4b\u2192clean.bwa.snp.vcf<o:p><\/o:p><\/p>\n<p>\u8fd9\u91cc\u5c0f\u679c\u5efa\u8bae\u5927\u5bb6\u4f7f\u7528mobaXterm\u6765\u8fde\u63a5linux\u670d\u52a1\u5668\uff0c\u56e0\u4e3amobaXterm\u53ef\u4ee5\u76f4\u63a5\u5728\u547d\u4ee4\u884c\u7a97\u53e3\u5de6\u8fb9\u5de5\u5177\u680f\u5b9e\u73b0\u6570\u636e\u7684\u4e0a\u4f20\u548c\u4e0b\u8f7d\u7b49\u529f\u80fd\uff0c\u975e\u5e38\u7b80\u5355\u65b9\u4fbf\uff0c\u5982\u4e0b\u56fe\u8fd9\u4e2a\u6846\u4e2d\u7684\u7bad\u5934\u5c31\u662f\u6570\u636e\u4e0a\u4f20\u6309\u94ae\uff0c\u800c\u5b83\u5de6\u8fb9\u53e6\u4e00\u4e2a\u7bad\u5934\u5c31\u662f\u6570\u636e\u4e0b\u8f7d\u6309\u94ae\u3002\u521b\u5efa\u6210\u529f\u540e\u901a\u8fc7\u5de6\u4fa7\u5de5\u5177\u680f\u5c06\u4e0b\u673a\u6570\u636e\u62f7\u8d1d\u81f3\u65b0\u5efa\u76ee\u5f55raw_data\uff1a<\/p>\n<p><span lang=\"EN-US\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.15535714285714286\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_sqVxPqL8iccwOiaSCzZJDSXn9L5ArA-2.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaib70dAzgtWRqsNcsNOjNKTcxG5wYsqVxPqL8iccwOiaSCzZJDSXn9L5ArA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"560\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><span style=\"display: none;line-height: 0px;\">\u200d<\/span><\/p>\n<h1><br \/><\/h1>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"properties\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u4f7f\u7528SOAPnuke\u8fc7\u6ee4\u6570\u636e\uff08\u6ce8\u610f\uff0c\u6b64\u547d\u4ee4\u4e3a\u8fde\u7eed\u4e00\u884c\uff09<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">SOAPnuke<\/span> <span class=\"code-snippet__string\">filter -1 .\/raw_data\/G1211112269_Barcode_e.fq.gz -l 10 -q 0.5 -n 0.01 -T 1 -o .\/01_clean\/ -C test_clean.fq.gz<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u53ef\u572801_clean\u8def\u5f84\u4e0b\u4f7f\u7528less\u547d\u4ee4\u9010\u4e00\u67e5\u770b\u6d4b\u5e8f\u6570\u636e\u8d28\u91cf\u8bc4\u4f30\u8868\uff0c\u7b80\u5355\u5c55\u793a\u8fc7\u6ee4\u524d\u540e\u6570\u636e\u603b\u4f53\u60c5\u51b5\u5982\u4e0b<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">less<\/span> <span class=\"code-snippet__string\">01_clean\/Basic_Statistics_of_Sequencing_Quality.txt<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3982142857142857\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_qf8OYaSGPjBDuPw2CSWkqB29Q8VGMA-2.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibXPhibPHTa1IONBQPvCAaqMOlLqf8OYaSGPjBDuPw2CSWkqB29Q8VGMA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"560\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p>\u5927\u5bb6\u4e5f\u53ef\u4ee5\u628a\u6570\u636e\u4e0b\u8f7d\u5230\u672c\u5730\u8fdb\u884c\u67e5\u770b\uff0c\u53ef\u572801_clean\u8def\u5f84\u4e0b\u627e\u5230”Basic_Statistics_of_Sequencing_Quality.txt”\u6587\u4ef6\u3002\u8be5\u6587\u4ef6\u63d0\u4f9b\u4e86\u8fc7\u6ee4\u524d\u540e\u6570\u636e\u8d28\u91cf\u7684\u5bf9\u6bd4\u7edf\u8ba1\u4fe1\u606f\uff0c\u53ef\u4ee5\u67e5\u770b\u4ee5\u4e0b\u6307\u6807\uff1a\u6570\u636e\u603b\u6570\u3001\u603b\u78b1\u57fa\u6570\u3001GC\u542b\u91cf\u3001Q20\u548cQ30\u7684\u6bd4\u4f8b\u7b49\uff0c\u53ef\u901a\u8fc7\u67e5\u770b\u8be5\u6587\u4ef6\u4e86\u89e3\u6570\u636e\u8d28\u91cf\u4e0e \u8fc7\u6ee4\u60c5\u51b5\uff0c\u622a\u56fe\u5982\u4e0b<span lang=\"EN-US\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5214285714285715\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_CqQICEcWzya1L2SP7BfN5IFIHwbS4w-2.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibpULaBNfQgQz30r1d3jmmQe4ACqQICEcWzya1L2SP7BfN5IFIHwbS4w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"560\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><br \/><\/p>\n<p><br \/><\/p>\n<p>\u5230\u8fd9\u91cc\u4e3a\u6b62\u8d28\u91cf\u8bc4\u4f30\u7684\u64cd\u4f5c\u5c31\u7ed3\u675f\u5566\uff0c\u662f\u4e0d\u662f\u975e\u5e38\u7b80\u5355\u5462\uff1f\u4e0b\u4e00\u6b21\u5c0f\u82b1\u4f1a\u8be6\u7ec6\u7ed9\u5927\u5bb6\u4ecb\u7ecd\u5982\u4f55\u89c2\u770b\u8d28\u91cf\u8bc4\u4f30\u7ed3\u679c\u4ee5\u53ca\u751f\u6210\u7684\u8d28\u91cf\u8bc4\u4f30\u62a5\u544a\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u4eec\u4e00\u5b9a\u8981\u7ee7\u7eed\u5173\u6ce8\u54e6~<span lang=\"EN-US\"><\/span><\/p>\n<p><span lang=\"EN-US\"><o:p><\/o:p><\/span><\/p>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 98%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;border-style: solid;border-width: 2px;border-color: rgb(160, 160, 160);padding: 27px;\">\n<section style=\"text-align: justify;line-height: 1.6;letter-spacing: 0px;padding-right: 6px;padding-left: 6px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14px;letter-spacing: 0.578px;\">\u5230\u8fd9\u91cc\u4e3a\u6b62\u8d28\u91cf\u8bc4\u4f30\u7684\u64cd\u4f5c\u5c31\u7ed3\u675f\u5566\uff0c\u662f\u4e0d\u662f\u975e\u5e38\u7b80\u5355\u5462\uff1f\u4e0b\u4e00\u6b21\u5c0f\u82b1\u4f1a\u8be6\u7ec6\u7ed9\u5927\u5bb6\u4ecb\u7ecd\u5982\u4f55\u89c2\u770b\u8d28\u91cf\u8bc4\u4f30\u7ed3\u679c\u4ee5\u53ca\u751f\u6210\u7684\u8d28\u91cf\u8bc4\u4f30\u62a5\u544a\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u4eec\u4e00\u5b9a\u8981\u7ee7\u7eed\u5173\u6ce8\u54e6<\/span><span style=\"font-size: 14px;letter-spacing: 0.578px;\">~<\/span><\/p>\n<p><span style=\"font-size: 14px;letter-spacing: 0.578px;\"><br \/><\/span><\/p>\n<p style=\"text-align: justify;\">\u5982\u679c\u5c0f\u4f19\u4f34\u4eec\u5e73\u65f6\u5728\u751f\u4fe1\u5206\u6790\u7684\u64cd\u4f5c\u8fc7\u7a0b\u4e2d\u9047\u5230\u56f0\u96be\uff0c\u6b22\u8fce\u5927\u5bb6\u4f7f\u7528\u5c0f\u82b1\u5f00\u53d1\u7684\u751f\u4fe1\u5de5\u5177\u5e73\u53f0http:\/\/www.biocloudservice.com\/home.html\uff0c\u5927\u5bb6\u5728\u65b0\u63a5\u89e6\u4e00\u4e2a\u77e5\u8bc6\u7684\u65f6\u5019\uff0c\u4e0e\u5176\u5148\u82b1\u8d39\u5927\u91cf\u65f6\u95f4\u6b7b\u5543\u77e5\u8bc6\u70b9\uff0c\u4e0d\u5982\u5148\u5229\u7528\u597d\u5de5\u5177\u5148\u81ea\u5df1\u4e0a\u624b\u63a5\u89e6\u6d41\u7a0b\uff0c\u5728\u8dd1\u5b8c\u4e00\u904d\u5168\u6d41\u7a0b\u540e\u518d\u8fd4\u56de\u53bb\u7406\u89e3\u77e5\u8bc6\u70b9\uff0c\u76f8\u4fe1\u53ef\u4ee5\u66f4\u597d\u66f4\u5feb\u5730\u7406\u89e3\uff0c\u8fbe\u5230\u4e8b\u534a\u529f\u500d\u7684\u6548\u679c\uff01<span lang=\"EN-US\"><o:p><\/o:p><\/span><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;font-size: 16px;letter-spacing: 0.578px;white-space: normal;text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;vertical-align: middle;width: 40.6667px;align-self: center;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7434944237918215\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_DsGlqEbyCicrBvWND6p21siabffBDA-2.gif?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_gif\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibyrlnYHKpXHqd0HWVnWKG2jCwfibDsGlqEbyCicrBvWND6p21siabffBDA\/640?wx_fmt=gif'\" data-type=\"gif\" data-w=\"269\" style=\"vertical-align: middle;width: 40.6667px;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 177.948px;align-self: center;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"178\" data-cropsely1=\"0\" data-cropsely2=\"53\" data-ratio=\"1\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_g3QkgKRyGrcXuVcRuy0TxF7QbOA5lQ-2.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibOCAs16wqcbibQF52pia3AsicicM2g3QkgKRyGrcXuVcRuy0TxF7QbOA5lQ\/640?wx_fmt=jpeg'\" data-type=\"gif\" data-w=\"796\" style=\"height: 178px;vertical-align: middle;width: 178px;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 40.6667px;align-self: center;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"0.7434944237918215\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_rL4tsfT96vpzIsHBrsoQqDuUUldFAg-2.gif?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_gif\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibkGkZ7Bia7bSTtFPQKYrBvKcVArL4tsfT96vpzIsHBrsoQqDuUUldFAg\/640?wx_fmt=gif'\" data-type=\"gif\" data-w=\"269\" style=\"vertical-align: middle;width: 40.6667px;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;font-size: 16px;letter-spacing: 0.578px;white-space: normal;text-align: center;\">\n<p><strong>\u957f\u6309\u8bc6\u522b\u4e8c\u7ef4\u7801\u5173\u6ce8\u6211\u4eec\u54df<\/strong><\/p>\n<\/section>\n<section style=\"text-align: justify;line-height: 1.6;letter-spacing: 0px;padding-right: 6px;padding-left: 6px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 26px;height: 26px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);border-left: 1px solid rgb(160, 160, 160);border-bottom-left-radius: 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650761303&idx=2&sn=6f73471d1e82c56c0b2160d0fcd32d53&scene=21#wechat_redirect\" textvalue=\"\" linktype=\"image\" imgurl=\"https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/0?wx_fmt=jpeg\" imgdata=\"[object Object]\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.35083798882681566\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_pBN4r4MaBCk9p9v16yZwvMvS5nM7sQ-2.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"895\" style=\"\" data-recalc-dims=\"1\" \/><\/span><\/a><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3488888888888889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_uKaqczIP6EMQhkT9sW7c1bKv6z9AAg-2.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdD9CpdQZl2iaySUTmNepgg2BpMuKaqczIP6EMQhkT9sW7c1bKv6z9AAg\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"900\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p powered-by=\"xiumi.us\">\uff08\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u8df3\u8f6c\uff09<\/p>\n<section style=\"text-align: left;margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;min-width: 10%;vertical-align: top;padding-right: 20px;\">\n<section style=\"transform: translate3d(20px, 0px, 0px);margin: 10px 0% -30px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);text-align: justify;font-size: 12px;\">\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.866\" data-s=\"300,640\" 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data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_KoP0KgFxjKk4cgY8Jeh5Z19dSq0Xrg-2.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYywn1OQia51WibQZhibAV4c1icVMdHhzE6jfjR7uxo3fuwLyhnKoP0KgFxjKk4cgY8Jeh5Z19dSq0Xrg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"275\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>DNA\u751f\u4fe1\u5206\u6790\uff08\u4e8c\uff09\uff1a\u4fdd\u59c6\u7ea7\u6559\u7a0b\uff01\u4e00\u5206\u949f\u5b8c\u6210FASTQ\u6587\u4ef6\u8d28\u91cf\u8bc4\u4f30 \u5c0f\u82b1 \u751f\u4fe1\u679c&nbs 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170);\"><strong>\u4e3a\u4ec0\u4e48\u8981\u8fdb\u884c\u8d28\u91cf\u8bc4\u4f30\u548c\u9884\u5904\u7406\uff1f<\/strong><\/span><o:p><\/o:p><\/h2>\n<p>1.\u9519\u8bef\u8bc6\u522b\u548c\u4fee\u6b63\uff1aFASTQ\u6587\u4ef6\u5305\u542b\u4e86\u6d4b\u5e8f\u5b9e\u9a8c\u751f\u6210\u7684\u539f\u59cb\u78b1\u57fa\u5e8f\u5217\u53ca\u5176\u5bf9\u5e94\u7684\u8d28\u91cf\u503c\u3002\u8fd9\u4e9b\u8d28\u91cf\u503c\u53cd\u6620\u4e86\u6bcf\u4e2a\u78b1\u57fa\u6d4b\u5e8f\u7684\u53ef\u4fe1\u5ea6\u3002\u6570\u636e\u8d28\u91cf\u8bc4\u4f30\u53ef\u4ee5\u5e2e\u52a9\u8bc6\u522b\u548c\u4fee\u6b63\u53ef\u80fd\u5b58\u5728\u7684\u6d4b\u5e8f\u9519\u8bef\uff0c\u4f8b\u5982\u6d4b\u5e8f\u78b1\u57fa\u66ff\u4ee3\u3001\u63d2\u5165\u6216\u5220\u9664\u9519\u8bef\u3002\u901a\u8fc7\u4fee\u590d\u8fd9\u4e9b\u9519\u8bef\uff0c\u53ef\u4ee5\u63d0\u9ad8\u540e\u7eed\u5206\u6790\u7684\u51c6\u786e\u6027\u3002<o:p><\/o:p><\/p>\n<p>2.\u5197\u4f59\u548c\u4f4e\u8d28\u91cf\u7247\u6bb5\u8fc7\u6ee4\uff1aFASTQ\u6587\u4ef6\u901a\u5e38\u5305\u542b\u5927\u91cf\u7684\u6d4b\u5e8f\u7247\u6bb5\uff0c\u5176\u4e2d\u53ef\u80fd\u5b58\u5728\u5197\u4f59\u5e8f\u5217\u3001PCR\u91cd\u590d\u6216\u8005\u4f4e\u8d28\u91cf\u7684\u7247\u6bb5\u3002\u5bf9\u6570\u636e\u8fdb\u884c\u8d28\u91cf\u8bc4\u4f30\u548c\u9884\u5904\u7406\u53ef\u4ee5\u8fc7\u6ee4\u6389\u8fd9\u4e9b\u4e0d\u5fc5\u8981\u7684\u7247\u6bb5\uff0c\u51cf\u5c11\u6570\u636e\u91cf\u548c\u566a\u97f3\uff0c\u63d0\u9ad8\u540e\u7eed\u5206\u6790\u7684\u6548\u7387\u548c\u51c6\u786e\u6027\u3002<o:p><\/o:p><\/p>\n<p>3.\u8d28\u91cf\u63a7\u5236\uff1a\u6570\u636e\u8d28\u91cf\u8bc4\u4f30\u53ef\u4ee5\u63d0\u4f9b\u5173\u4e8e\u6d4b\u5e8f\u6570\u636e\u8d28\u91cf\u7684\u8be6\u7ec6\u7edf\u8ba1\u4fe1\u606f\uff0c\u4f8b\u5982\u6bcf\u4e2a\u78b1\u57fa\u7684\u8d28\u91cf\u5206\u5e03\u3001\u6d4b\u5e8f\u6df1\u5ea6\u7b49\u3002\u8fd9\u4e9b\u4fe1\u606f\u5bf9\u4e8e\u9009\u62e9\u5408\u9002\u7684\u9608\u503c\u3001\u786e\u5b9a\u5206\u6790\u65b9\u6cd5\u548c\u89e3\u91ca\u7ed3\u679c\u975e\u5e38\u91cd\u8981\u3002\u901a\u8fc7\u5bf9\u6570\u636e\u8d28\u91cf\u8fdb\u884c\u63a7\u5236\uff0c\u53ef\u4ee5\u786e\u4fdd\u540e\u7eed\u5206\u6790\u7684\u53ef\u9760\u6027\u548c\u53ef\u91cd\u590d\u6027\u3002<o:p><\/o:p><\/p>\n<p>4.\u6570\u636e\u6e05\u6d17\uff1a\u5728\u8fdb\u884c\u67d0\u4e9b\u5206\u6790\u4e4b\u524d\uff0c\u9700\u8981\u5bf9\u6570\u636e\u8fdb\u884c\u6e05\u6d17\uff0c\u4ee5\u53bb\u9664\u4f4e\u8d28\u91cf\u7684\u78b1\u57fa\u6216\u8005\u4e0d\u53ef\u9760\u7684\u7247\u6bb5\u3002\u8fd9\u6837\u53ef\u4ee5\u6392\u9664\u566a\u97f3\u5f15\u5165\u548c\u504f\u5dee\uff0c\u786e\u4fdd\u5206\u6790\u7ed3\u679c\u66f4\u52a0\u51c6\u786e\u548c\u53ef\u9760\u3002<span style=\"mso-bookmark:\u4e3a\u4ec0\u4e48\u8981\u8fdb\u884c\u8d28\u91cf\u8bc4\u4f30\u548c\u9884\u5904\u7406;\"><\/span><\/p>\n<p><span style=\"mso-bookmark:\u4e3a\u4ec0\u4e48\u8981\u8fdb\u884c\u8d28\u91cf\u8bc4\u4f30\u548c\u9884\u5904\u7406;\"><span lang=\"EN-US\"><o:p><\/o:p><\/span><\/span><\/p>\n<h2><strong><span style=\"color: rgb(2, 30, 170);\">FASTQ<\/span><\/strong><strong><span style=\"color: rgb(2, 30, 170);\">\u6570\u636e\u8d28\u63a7\u8fc7\u6ee4\u8f6f\u4ef6\u2014SOAPnuke<\/span><\/strong><\/h2>\n<h2><o:p><\/o:p><\/h2>\n<p>\u5f53\u4eca\u5e02\u9762\u4e0a\u6709\u8bb8\u591a\u53ef\u4ee5\u5bf9FASTQ\u6570\u636e\u8fdb\u884c\u8d28\u63a7\u8fc7\u6ee4\u7684\u8f6f\u4ef6\uff0cSOAPnuke\u662f\u5176\u4e2d\u5bf9\u65b0\u624b\u6bd4\u8f83\u53cb\u597d\uff0c\u4f7f\u7528\u8d77\u6765\u6bd4\u8f83\u7b80\u5355\u7684\u5165\u95e8\u7ea7\u8f6f\u4ef6\u3002\u5c0f\u679c\u8fd9\u6b21\u5c31\u5728linux\u4e2d\u4ee5SOAPnuke\u4e3a\u4f8b\uff0c\u6559\u7ed9\u5927\u5bb6\u8d28\u91cf\u8bc4\u4f30\u7684\u6b65\u9aa4\u3002<o:p><\/o:p><\/p>\n<p>\u4e0b\u9762\u662fSOAPnuke\u7684\u4e00\u4e9b\u5e38\u7528\u53c2\u6570\u7684\u4ecb\u7ecd\uff1a<o:p><\/o:p><\/p>\n<p>1.-1\uff1a\u6307\u5b9a\u5f85\u5904\u7406\u6570\u636e\u7684\u7b2c\u4e00\u5bf9\uff08\u6216\u552f\u4e00\uff09\u6d4b\u5e8f\u6587\u4ef6\u7684\u8def\u5f84\u3002<o:p><\/o:p><\/p>\n<p>2.-2\uff1a\u6307\u5b9a\u5f85\u5904\u7406\u6570\u636e\u7684\u7b2c\u4e8c\u5bf9\u6d4b\u5e8f\u6587\u4ef6\u7684\u8def\u5f84\uff08\u5982\u679c\u662f\u53cc\u7aef\u6d4b\u5e8f\u6570\u636e\uff09\u3002<o:p><\/o:p><\/p>\n<p>3.-r\uff1a\u6307\u5b9a\u4fdd\u5b58\u5904\u7406\u7ed3\u679c\u7684\u8def\u5f84\u3002<o:p><\/o:p><\/p>\n<p>4.-C\uff1a\u7528\u4e8e\u6307\u5b9a\u914d\u7f6e\u6587\u4ef6\u7684\u8def\u5f84\u3002\u914d\u7f6e\u6587\u4ef6\u5305\u542b\u4e86SOAPnuke\u7684\u8be6\u7ec6\u8bbe\u7f6e\uff0c\u5982\u5904\u7406\u8fc7\u7a0b\u4e2d\u6240\u4f7f\u7528\u7684\u8d28\u91cf\u8ba1\u7b97\u65b9\u6cd5\u3001\u8d28\u91cf\u9608\u503c\u7b49\u3002\u53ef\u4ee5\u6839\u636e\u9700\u8981\u8fdb\u884c\u81ea\u5b9a\u4e49\u8bbe\u7f6e\u3002<o:p><\/o:p><\/p>\n<p>5.-5\uff1a\u6307\u5b9a5’\u7aef\u526a\u5207\u7684\u78b1\u57fa\u6570\u3002\u8be5\u53c2\u6570\u7528\u4e8e\u53bb\u9664\u6d4b\u5e8f\u6587\u5e93\u4e2d\u7684\u4f4e\u8d28\u91cf\u672b\u7aef\uff0c\u63d0\u9ad8\u6570\u636e\u8d28\u91cf\u3002<o:p><\/o:p><\/p>\n<p>6.-3\uff1a\u6307\u5b9a3’\u7aef\u526a\u5207\u7684\u78b1\u57fa\u6570\u3002\u8be5\u53c2\u6570\u540c\u6837\u7528\u4e8e\u53bb\u9664\u6d4b\u5e8f\u6587\u5e93\u4e2d\u7684\u4f4e\u8d28\u91cf\u672b\u7aef\u3002<o:p><\/o:p><\/p>\n<p>7.-l\uff1a\u6307\u5b9a\u6700\u77ed\u6709\u6548\u8bfb\u957f\uff08clean read length\uff09\u3002\u8be5\u53c2\u6570\u7528\u4e8e\u53bb\u9664\u8f83\u77ed\u7684reads\uff0c\u63d0\u9ad8\u6570\u636e\u8d28\u91cf\u3002<o:p><\/o:p><\/p>\n<p>8.-q\uff1a\u6307\u5b9a\u8d28\u91cf\u9608\u503c\u3002\u8be5\u53c2\u6570\u7528\u4e8e\u5c06\u4f4e\u4e8e\u6307\u5b9a\u8d28\u91cf\u9608\u503c\u7684\u78b1\u57fa\u6807\u8bb0\u4e3aN\uff0c\u4ece\u800c\u53bb\u9664\u4f4e\u8d28\u91cf\u7684\u78b1\u57fa\u3002<o:p><\/o:p><\/p>\n<p>9.-n\uff1a\u6307\u5b9a\u5141\u8bb8\u7684\u6700\u5927\u8fde\u7eed\u4f4e\u8d28\u91cf\u78b1\u57fa\u6570\u3002\u8be5\u53c2\u6570\u7528\u4e8e\u53bb\u9664\u5177\u6709\u8fde\u7eed\u4f4e\u8d28\u91cf\u78b1\u57fa\u7684reads\u3002<o:p><\/o:p><\/p>\n<p>10.-M\uff1a\u6307\u5b9a\u4fdd\u7559\u7684\u6700\u5c0f\u5e73\u5747\u8d28\u91cf\u503c\u3002\u8be5\u53c2\u6570\u7528\u4e8e\u8fc7\u6ee4\u6389\u5e73\u5747\u8d28\u91cf\u4f4e\u4e8e\u6307\u5b9a\u9608\u503c\u7684reads\u3002<o:p><\/o:p><\/p>\n<p>11.-d\uff1a\u6307\u5b9aadapter\u5e8f\u5217\u6587\u4ef6\u7684\u8def\u5f84\u3002\u8be5\u53c2\u6570\u7528\u4e8e\u53bb\u9664reads\u4e2d\u7684adapter\u5e8f\u5217\u3002<\/p>\n<p>12.-t\uff1a\u6307\u5b9a\u7ebf\u7a0b\u6570\u3002\u8be5\u53c2\u6570\u7528\u4e8e\u6307\u5b9a\u5e76\u884c\u5904\u7406\u7684\u7ebf\u7a0b\u6570\uff0c\u52a0\u5feb\u5904\u7406\u901f\u5ea6\u3002<o:p><\/o:p><\/p>\n<p>13.-D\uff1a\u5f00\u542fadapter\u5e8f\u5217\u7684\u5b58\u5728\u6027\u68c0\u6d4b\u548c\u53bb\u9664\u3002<o:p><\/o:p><\/p>\n<p>14.-I\uff1a\u5f00\u542fPCR\u5197\u4f59\u5e8f\u5217\u7684\u68c0\u6d4b\u548c\u53bb\u9664\u3002<o:p><\/o:p><\/p>\n<p>15.-O\uff1a\u5f00\u542fPCR\u5197\u4f59\u5e8f\u5217\u914d\u5bf9\u6bd4\u5bf9\u7684\u68c0\u6d4b\u548c\u53bb\u9664\u3002<o:p><\/o:p><\/p>\n<p>\u8fd9\u4e9b\u53ea\u662fSOAPnuke\u7684\u4e00\u4e9b\u5e38\u7528\u53c2\u6570\uff0c\u8fd8\u6709\u5176\u4ed6\u4e00\u4e9b\u53ef\u9009\u53c2\u6570\u7528\u4e8e\u66f4\u8be6\u7ec6\u5730\u63a7\u5236\u9884\u5904\u7406\u8fc7\u7a0b\u3002\u4f7f\u7528\u9002\u5f53\u7684\u53c2\u6570\u8bbe\u7f6e\u53ef\u4ee5\u6839\u636e\u5b9e\u9645\u9700\u6c42\u8fdb\u884c\u6570\u636e\u8d28\u91cf\u63a7\u5236\u548c\u9884\u5904\u7406\uff0c\u63d0\u9ad8\u540e\u7eed\u5206\u6790\u7684\u51c6\u786e\u6027\u548c\u53ef\u9760\u6027\u3002\u5c0f\u4f19\u4f34\u4eec\u5982\u679c\u60f3\u4e86\u89e3\u7684\u8bdd\u53ef\u4ee5\u5728\u547d\u4ee4\u884c\u4e2d\u8fd0\u884cSOAPnuke filter \u2013help\uff0c\u67e5\u770bSOAPnuke\u66f4\u591a\u5b8c\u6574\u53c2\u6570\u3002<o:p><\/o:p><\/p>\n<p><br \/><\/p>\n<h2><span style=\"color: rgb(2, 30, 170);\"><strong>\u64cd\u4f5c\u6b65\u9aa4<\/strong><\/span><span lang=\"EN-US\"><o:p><\/o:p><\/span><\/h2>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u521b\u5efa\u6587\u4ef6\u5939<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">mkdir<\/span> <span class=\"code-snippet__number\">00_<\/span><span class=\"code-snippet__keyword\">ref<\/span> <span class=\"code-snippet__number\">01_<\/span>clean <span class=\"code-snippet__number\">02_<\/span>align <span class=\"code-snippet__number\">03_<\/span>SNPCalling <span class=\"code-snippet__number\">04_<\/span>annotation raw_data<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p>\u4e4b\u6240\u4ee5\u521b\u5efa\u8fd9\u4e48\u591a\u6587\u4ef6\u5939\u662f\u56e0\u4e3a\u8d28\u91cf\u8bc4\u4f30\u53ea\u662f\u6211\u4eec\u5206\u6790\u6d41\u7a0b\u7684\u7b2c\u4e00\u6b65\uff0c\u5728\u540e\u7eed\u5206\u6790\u6d41\u7a0b\u8fd8\u4f1a\u4ea7\u751f\u4ee5\u4e0b\u6587\u4ef6\uff1a<o:p><\/o:p><\/p>\n<p>\u8fc7\u6ee4\u2192clean.fq\u2192\u6bd4\u5bf9\u2192clean.bwa.sam\u2192\u6570\u636e\u683c\u5f0f\u8f6c\u6362\u2192clean.bwa.bam\u2192\u7d22\u5f15\u7ed3\u679c\u6392\u5e8f\u2192clean.bwa.sorted.bam\u2192\u53d8\u5f02\u68c0\u6d4b\u2192clean.bwa.snp.vcf<o:p><\/o:p><\/p>\n<p>\u8fd9\u91cc\u5c0f\u679c\u5efa\u8bae\u5927\u5bb6\u4f7f\u7528mobaXterm\u6765\u8fde\u63a5linux\u670d\u52a1\u5668\uff0c\u56e0\u4e3amobaXterm\u53ef\u4ee5\u76f4\u63a5\u5728\u547d\u4ee4\u884c\u7a97\u53e3\u5de6\u8fb9\u5de5\u5177\u680f\u5b9e\u73b0\u6570\u636e\u7684\u4e0a\u4f20\u548c\u4e0b\u8f7d\u7b49\u529f\u80fd\uff0c\u975e\u5e38\u7b80\u5355\u65b9\u4fbf\uff0c\u5982\u4e0b\u56fe\u8fd9\u4e2a\u6846\u4e2d\u7684\u7bad\u5934\u5c31\u662f\u6570\u636e\u4e0a\u4f20\u6309\u94ae\uff0c\u800c\u5b83\u5de6\u8fb9\u53e6\u4e00\u4e2a\u7bad\u5934\u5c31\u662f\u6570\u636e\u4e0b\u8f7d\u6309\u94ae\u3002\u521b\u5efa\u6210\u529f\u540e\u901a\u8fc7\u5de6\u4fa7\u5de5\u5177\u680f\u5c06\u4e0b\u673a\u6570\u636e\u62f7\u8d1d\u81f3\u65b0\u5efa\u76ee\u5f55raw_data\uff1a<\/p>\n<p><span lang=\"EN-US\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.15535714285714286\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_sqVxPqL8iccwOiaSCzZJDSXn9L5ArA-2.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaib70dAzgtWRqsNcsNOjNKTcxG5wYsqVxPqL8iccwOiaSCzZJDSXn9L5ArA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"560\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><span style=\"display: none;line-height: 0px;\">\u200d<\/span><\/p>\n<h1><br \/><\/h1>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"properties\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u4f7f\u7528SOAPnuke\u8fc7\u6ee4\u6570\u636e\uff08\u6ce8\u610f\uff0c\u6b64\u547d\u4ee4\u4e3a\u8fde\u7eed\u4e00\u884c\uff09<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">SOAPnuke<\/span> <span class=\"code-snippet__string\">filter -1 .\/raw_data\/G1211112269_Barcode_e.fq.gz -l 10 -q 0.5 -n 0.01 -T 1 -o .\/01_clean\/ -C test_clean.fq.gz<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u53ef\u572801_clean\u8def\u5f84\u4e0b\u4f7f\u7528less\u547d\u4ee4\u9010\u4e00\u67e5\u770b\u6d4b\u5e8f\u6570\u636e\u8d28\u91cf\u8bc4\u4f30\u8868\uff0c\u7b80\u5355\u5c55\u793a\u8fc7\u6ee4\u524d\u540e\u6570\u636e\u603b\u4f53\u60c5\u51b5\u5982\u4e0b<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">less<\/span> <span class=\"code-snippet__string\">01_clean\/Basic_Statistics_of_Sequencing_Quality.txt<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3982142857142857\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_qf8OYaSGPjBDuPw2CSWkqB29Q8VGMA-2.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibXPhibPHTa1IONBQPvCAaqMOlLqf8OYaSGPjBDuPw2CSWkqB29Q8VGMA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"560\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p>\u5927\u5bb6\u4e5f\u53ef\u4ee5\u628a\u6570\u636e\u4e0b\u8f7d\u5230\u672c\u5730\u8fdb\u884c\u67e5\u770b\uff0c\u53ef\u572801_clean\u8def\u5f84\u4e0b\u627e\u5230”Basic_Statistics_of_Sequencing_Quality.txt”\u6587\u4ef6\u3002\u8be5\u6587\u4ef6\u63d0\u4f9b\u4e86\u8fc7\u6ee4\u524d\u540e\u6570\u636e\u8d28\u91cf\u7684\u5bf9\u6bd4\u7edf\u8ba1\u4fe1\u606f\uff0c\u53ef\u4ee5\u67e5\u770b\u4ee5\u4e0b\u6307\u6807\uff1a\u6570\u636e\u603b\u6570\u3001\u603b\u78b1\u57fa\u6570\u3001GC\u542b\u91cf\u3001Q20\u548cQ30\u7684\u6bd4\u4f8b\u7b49\uff0c\u53ef\u901a\u8fc7\u67e5\u770b\u8be5\u6587\u4ef6\u4e86\u89e3\u6570\u636e\u8d28\u91cf\u4e0e \u8fc7\u6ee4\u60c5\u51b5\uff0c\u622a\u56fe\u5982\u4e0b<span lang=\"EN-US\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5214285714285715\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_CqQICEcWzya1L2SP7BfN5IFIHwbS4w-2.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibpULaBNfQgQz30r1d3jmmQe4ACqQICEcWzya1L2SP7BfN5IFIHwbS4w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"560\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><br \/><\/p>\n<p><br \/><\/p>\n<p>\u5230\u8fd9\u91cc\u4e3a\u6b62\u8d28\u91cf\u8bc4\u4f30\u7684\u64cd\u4f5c\u5c31\u7ed3\u675f\u5566\uff0c\u662f\u4e0d\u662f\u975e\u5e38\u7b80\u5355\u5462\uff1f\u4e0b\u4e00\u6b21\u5c0f\u82b1\u4f1a\u8be6\u7ec6\u7ed9\u5927\u5bb6\u4ecb\u7ecd\u5982\u4f55\u89c2\u770b\u8d28\u91cf\u8bc4\u4f30\u7ed3\u679c\u4ee5\u53ca\u751f\u6210\u7684\u8d28\u91cf\u8bc4\u4f30\u62a5\u544a\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u4eec\u4e00\u5b9a\u8981\u7ee7\u7eed\u5173\u6ce8\u54e6~<span lang=\"EN-US\"><\/span><\/p>\n<p><span lang=\"EN-US\"><o:p><\/o:p><\/span><\/p>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 98%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;border-style: solid;border-width: 2px;border-color: rgb(160, 160, 160);padding: 27px;\">\n<section style=\"text-align: justify;line-height: 1.6;letter-spacing: 0px;padding-right: 6px;padding-left: 6px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14px;letter-spacing: 0.578px;\">\u5230\u8fd9\u91cc\u4e3a\u6b62\u8d28\u91cf\u8bc4\u4f30\u7684\u64cd\u4f5c\u5c31\u7ed3\u675f\u5566\uff0c\u662f\u4e0d\u662f\u975e\u5e38\u7b80\u5355\u5462\uff1f\u4e0b\u4e00\u6b21\u5c0f\u82b1\u4f1a\u8be6\u7ec6\u7ed9\u5927\u5bb6\u4ecb\u7ecd\u5982\u4f55\u89c2\u770b\u8d28\u91cf\u8bc4\u4f30\u7ed3\u679c\u4ee5\u53ca\u751f\u6210\u7684\u8d28\u91cf\u8bc4\u4f30\u62a5\u544a\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u4eec\u4e00\u5b9a\u8981\u7ee7\u7eed\u5173\u6ce8\u54e6<\/span><span style=\"font-size: 14px;letter-spacing: 0.578px;\">~<\/span><\/p>\n<p><span style=\"font-size: 14px;letter-spacing: 0.578px;\"><br \/><\/span><\/p>\n<p style=\"text-align: justify;\">\u5982\u679c\u5c0f\u4f19\u4f34\u4eec\u5e73\u65f6\u5728\u751f\u4fe1\u5206\u6790\u7684\u64cd\u4f5c\u8fc7\u7a0b\u4e2d\u9047\u5230\u56f0\u96be\uff0c\u6b22\u8fce\u5927\u5bb6\u4f7f\u7528\u5c0f\u82b1\u5f00\u53d1\u7684\u751f\u4fe1\u5de5\u5177\u5e73\u53f0http:\/\/www.biocloudservice.com\/home.html\uff0c\u5927\u5bb6\u5728\u65b0\u63a5\u89e6\u4e00\u4e2a\u77e5\u8bc6\u7684\u65f6\u5019\uff0c\u4e0e\u5176\u5148\u82b1\u8d39\u5927\u91cf\u65f6\u95f4\u6b7b\u5543\u77e5\u8bc6\u70b9\uff0c\u4e0d\u5982\u5148\u5229\u7528\u597d\u5de5\u5177\u5148\u81ea\u5df1\u4e0a\u624b\u63a5\u89e6\u6d41\u7a0b\uff0c\u5728\u8dd1\u5b8c\u4e00\u904d\u5168\u6d41\u7a0b\u540e\u518d\u8fd4\u56de\u53bb\u7406\u89e3\u77e5\u8bc6\u70b9\uff0c\u76f8\u4fe1\u53ef\u4ee5\u66f4\u597d\u66f4\u5feb\u5730\u7406\u89e3\uff0c\u8fbe\u5230\u4e8b\u534a\u529f\u500d\u7684\u6548\u679c\uff01<span lang=\"EN-US\"><o:p><\/o:p><\/span><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;font-size: 16px;letter-spacing: 0.578px;white-space: normal;text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;vertical-align: middle;width: 40.6667px;align-self: center;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7434944237918215\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_DsGlqEbyCicrBvWND6p21siabffBDA-2.gif?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_gif\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibyrlnYHKpXHqd0HWVnWKG2jCwfibDsGlqEbyCicrBvWND6p21siabffBDA\/640?wx_fmt=gif'\" data-type=\"gif\" data-w=\"269\" style=\"vertical-align: middle;width: 40.6667px;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 177.948px;align-self: center;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"178\" data-cropsely1=\"0\" data-cropsely2=\"53\" data-ratio=\"1\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_g3QkgKRyGrcXuVcRuy0TxF7QbOA5lQ-2.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibOCAs16wqcbibQF52pia3AsicicM2g3QkgKRyGrcXuVcRuy0TxF7QbOA5lQ\/640?wx_fmt=jpeg'\" data-type=\"gif\" data-w=\"796\" style=\"height: 178px;vertical-align: middle;width: 178px;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 40.6667px;align-self: center;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"0.7434944237918215\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_rL4tsfT96vpzIsHBrsoQqDuUUldFAg-2.gif?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_gif\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibkGkZ7Bia7bSTtFPQKYrBvKcVArL4tsfT96vpzIsHBrsoQqDuUUldFAg\/640?wx_fmt=gif'\" data-type=\"gif\" data-w=\"269\" style=\"vertical-align: middle;width: 40.6667px;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;font-size: 16px;letter-spacing: 0.578px;white-space: normal;text-align: center;\">\n<p><strong>\u957f\u6309\u8bc6\u522b\u4e8c\u7ef4\u7801\u5173\u6ce8\u6211\u4eec\u54df<\/strong><\/p>\n<\/section>\n<section style=\"text-align: justify;line-height: 1.6;letter-spacing: 0px;padding-right: 6px;padding-left: 6px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 26px;height: 26px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);border-left: 1px solid rgb(160, 160, 160);border-bottom-left-radius: 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650761303&idx=2&sn=6f73471d1e82c56c0b2160d0fcd32d53&scene=21#wechat_redirect\" textvalue=\"\" linktype=\"image\" imgurl=\"https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/0?wx_fmt=jpeg\" imgdata=\"[object Object]\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.35083798882681566\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_pBN4r4MaBCk9p9v16yZwvMvS5nM7sQ-2.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"895\" style=\"\" data-recalc-dims=\"1\" \/><\/span><\/a><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3488888888888889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_uKaqczIP6EMQhkT9sW7c1bKv6z9AAg-2.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdD9CpdQZl2iaySUTmNepgg2BpMuKaqczIP6EMQhkT9sW7c1bKv6z9AAg\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"900\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p powered-by=\"xiumi.us\">\uff08\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u8df3\u8f6c\uff09<\/p>\n<section style=\"text-align: left;margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;min-width: 10%;vertical-align: top;padding-right: 20px;\">\n<section style=\"transform: translate3d(20px, 0px, 0px);margin: 10px 0% -30px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);text-align: justify;font-size: 12px;\">\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.866\" data-s=\"300,640\" 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