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auto;height: auto;overflow: hidden;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<h1><\/h1>\n<h1><span style=\"color: rgb(2, 30, 170);\"><strong>\u4e00\u5206\u949f\u5b66\u4f1a\u5982\u4f55\u5904\u7406FASTQ\u6587\u4ef6<\/strong><\/span><o:p><\/o:p><\/h1>\n<p><span style=\"color: rgb(2, 30, 170);\"><strong><br \/><\/strong><\/span><\/p>\n<p>\u5728\u524d\u9762\u7684\u6587\u7ae0\u4e2d\uff0c\u5c0f\u82b1\u5411\u5927\u5bb6\u5206\u4eab\u4e86\u5355\u7ec6\u80de\u6d4b\u5e8f\u7684\u6d41\u7a0b\uff0c\u60f3\u5fc5\u5c0f\u4f19\u4f34\u4eec\u4e5f\u6210\u529f\u7684\u5b66\u4f1a\u4e86\u6e7f\u5b9e\u9a8c\u7684\u6b65\u9aa4\u5427\u3002\u4f46\u662f\u6e7f\u5b9e\u9a8c\u7684\u7ed3\u675f\u624d\u662f\u6574\u4e2a\u751f\u4fe1\u9879\u76ee\u7684\u5f00\u59cb\uff0c\u9762\u5bf9\u7740\u6d4b\u5e8f\u4eea\u4e0b\u673a\u7684\u539f\u59cb\u6570\u636e\uff0c\u5f88\u591a\u5c0f\u4f19\u4f34\u53ef\u80fd\u611f\u89c9\u65e0\u4ece\u4e0b\u624b\uff0c\u4e0d\u77e5\u9053\u600e\u4e48\u5bf9\u6570\u636e\u8fdb\u884c\u5206\u6790\u3002\u8fd9\u4e00\u6b21\u5c0f\u82b1\u5c31\u8ddf\u5927\u5bb6\u5206\u4eab\u4e00\u4e0b\u600e\u4e48\u9605\u8bfb\u4ece\u6d4b\u5e8f\u4eea\u5f97\u5230\u7684FASTQ\u6587\u4ef6\uff0c\u5927\u5bb6\u5feb\u6765\u770b\u770b\u5427\uff01<o:p><\/o:p><\/p>\n<h2><span style=\"color: rgb(2, 30, 170);\"><strong>\u4ec0\u4e48\u662fFASTQ\u6587\u4ef6\uff1f<\/strong><\/span><o:p><\/o:p><\/h2>\n<p><span style=\"color: rgb(2, 30, 170);\"><strong><br \/><\/strong><\/span><\/p>\n<p>FASTQ\uff08Fast Quality\uff09\u6587\u4ef6\u662f\u4e00\u79cd\u5e38\u7528\u7684\u5b58\u50a8\u6d4b\u5e8f\u6570\u636e\u7684\u6587\u672c\u683c\u5f0f\uff0c\u6587\u4ef6\u540e\u7f00\u901a\u5e38\u4e3a.fastq\u6216.fq\u3002\u5b83\u901a\u5e38\u7528\u4e8e\u5b58\u50a8\u9ad8\u901a\u91cf\u6d4b\u5e8f\uff08\u5982DNA\u6d4b\u5e8f\u6216RNA\u6d4b\u5e8f\uff09\u5f97\u5230\u7684\u539f\u59cb\u6d4b\u5e8f\u7247\u6bb5\uff08reads\uff09\u7684\u5e8f\u5217\u548c\u5bf9\u5e94\u7684\u8d28\u91cf\u4fe1\u606f\u3002<o:p><\/o:p><\/p>\n<p>FASTQ\u6587\u4ef6\u7684\u6bcf\u4e2a\u6d4b\u5e8f\u7247\u6bb5\u901a\u5e38\u7531\u56db\u884c\u7ec4\u6210\uff0c\u5177\u4f53\u683c\u5f0f\u5982\u4e0b\uff1a<o:p><\/o:p><\/p>\n<p>1.\u7b2c\u4e00\u884c\u4ee5\u7b26\u53f7 “@” 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170);\"><strong>\u4ec0\u4e48\u662f\u8d28\u91cf\u503cQ\u548c\u8d28\u91cf\u5206\u6570Phred\uff1f<\/strong><\/span><\/h2>\n<p>\u8d28\u91cf\u503cQ\u8868\u793a\u4e86\u6d4b\u5e8f\u7247\u6bb5\u4e2d\u6bcf\u4e2a\u78b1\u57fa\u7684\u53ef\u4fe1\u5ea6\uff0c\u78b1\u57fa\u8d28\u91cf\u503cQ\u8ba1\u7b97\u516c\u5f0f\uff1a<\/p>\n<p><span lang=\"EN-US\"><o:p><\/o:p><\/span><\/p>\n<p><span lang=\"EN-US\" style=\"mso-no-proof:yes;\"><\/span><span lang=\"EN-US\"><o:p><\/o:p><\/span><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><br \/><\/section>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3459715639810427\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_Y6rGnZBtyHPNKk5cukEqf23RsoIsAA-6.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibBibxudZckMAw1qXpu3MVj9AiaNY6rGnZBtyHPNKk5cukEqf23RsoIsAA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"211\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p>\u5176\u4e2dp\u4e3a\u78b1\u57fa\u88ab\u8bc6\u522b\u9519\u8bef\u7684\u6982\u7387\u3002\u6839\u636e\u516c\u5f0f\u8ba1\u7b97\u6240\u5f97Q\u503c\u8d8a\u9ad8\uff0c\u4ee3\u8868\u78b1\u57fa\u88ab\u6d4b\u9519\u7684\u6982\u7387\u8d8a\u5c0f\u3002\u4f8b\u5982\u6d4b\u5e8f\u6570\u636e\u4e0b\u673a\u8d28\u91cf\u8bc4\u4ef7\u6307\u6807\u4e2d\uff0cQ20\uff0cQ30\uff0cQ40\u5c31\u5206\u522b\u4ee3\u8868\u6d4b\u5e8f\u9519\u8bef\u7387\u4e3a1%\uff0c0.1%\uff0c0.01%\u3002\u4e0b\u673a\u6570\u636eQ30 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data-w=\"560\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p>\u9700\u8981\u6ce8\u610f\u7684\u662f\uff0c\u7531\u4e8eFASTQ\u6587\u4ef6\u7684\u4f53\u79ef\u901a\u5e38\u8f83\u5927\uff0c\u5728\u5904\u7406\u548c\u4f20\u8f93\u65f6\u9700\u8981\u8003\u8651\u5b58\u50a8\u7a7a\u95f4\u548c\u8ba1\u7b97\u8d44\u6e90\u7684\u9650\u5236\u3002<o:p><\/o:p><\/p>\n<p>\u770b\u5230\u8fd9\u91cc\uff0c\u5c0f\u82b1\u76f8\u4fe1\u5927\u5bb6\u5df2\u7ecf\u638c\u63e1\u4e86\u5982\u4f55\u9605\u8bfbFASTQ\u6587\u4ef6\uff0c\u4f46\u662f\u53ef\u80fd\u8fd8\u6709\u5c0f\u4f19\u4f34\u4eec\u4f1a\u6709\u7591\u95ee\uff0cFASTQ\u6587\u4ef6\u53ef\u4ee5\u8fdb\u884c\u4ec0\u4e48\u5904\u7406\u5462\uff1f\u80fd\u591f\u53d8\u6210\u4ec0\u4e48\u6587\u4ef6\u5462\uff1f\u4e0e\u5176\u4ed6\u6587\u4ef6\u7c7b\u578b\u6709\u4ec0\u4e48\u5173\u7cfb\u5462\uff1f\u4e0d\u8981\u5fc3\u6025\uff0c\u5c0f\u679c\u8fd9\u5c31\u7ed9\u5927\u5bb6\u5a13\u5a13\u9053\u6765\u3002<o:p><\/o:p><\/p>\n<p><br \/><\/p>\n<h2><span style=\"color: rgb(2, 30, 170);\"><strong>FASTQ\u6587\u4ef6\u540e\u7eed\u5982\u4f55\u5904\u7406\uff1f<\/strong><\/span><o:p><\/o:p><\/h2>\n<p>\u521d\u5165\u751f\u4fe1\u7684\u5c0f\u4f19\u4f34\u4eec\u53ef\u80fd\u542c\u8bf4\u8fc7FASTA\u6587\u4ef6\uff08.fasta\/.fa)\uff0c\u4e0eFASTQ\u540d\u79f0\u6709\u516b\u6210\u751a\u81f3\u4e5d\u6210\u7684\u76f8\u4f3c\uff0c\u53ef\u80fd\u5f88\u591a\u5c0f\u4f19\u4f34\u90fd\u4e0d\u592a\u80fd\u5206\u6e05\u4ed6\u4eec\uff0c\u73b0\u5728\u5c31\u8ba9\u5c0f\u679c\u7528\u201c\u7167\u5996\u955c\u201d\u63ed\u5f00FASTQ\u6587\u4ef6\u7684\u670b\u53cb\u4eec\u7684\u795e\u79d8\u9762\u7eb1\u5427\uff1a<o:p><\/o:p><\/p>\n<p>\u4e3e\u4e00\u4e2aFASTA\u6587\u4ef6\u7684\u4f8b\u5b50\uff1a<\/p>\n<p><o:p><\/o:p><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5517857142857143\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_xFYHdDS2UP9LDEaUwVHZTtsJV2AnYA-6.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibkh7OBic2h8picqzMAshlQhKZrdxFYHdDS2UP9LDEaUwVHZTtsJV2AnYA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"560\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><br \/><\/p>\n<p><br 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style=\"letter-spacing: 0.034em;\"><\/span><o:p><\/o:p><\/p>\n<p>1.\u5bf9\u6bd4\u5bf9\u7ed3\u679c\u8fdb\u884c\u53d8\u5f02\u68c0\u6d4b\u65f6\uff0c\u53ef\u4ee5\u5c06SAM\/BAM\u6587\u4ef6\u4e0e\u53c2\u8003\u57fa\u56e0\u7ec4\u8fdb\u884c\u6bd4\u8f83\uff0c\u8bc6\u522b\u51fa\u6d4b\u5e8f\u6837\u672c\u4e2d\u4e0e\u53c2\u8003\u57fa\u56e0\u7ec4\u4e0d\u540c\u7684\u53d8\u5f02\u4f4d\u70b9\uff0c\u5e76\u751f\u6210Variant Call Format\uff08VCF\uff09\u6587\u4ef6\u3002<o:p><\/o:p><\/p>\n<p><br \/><\/p>\n<p>2.\u6839\u636eSAM\/BAM\u6587\u4ef6\u7684\u8986\u76d6\u7a0b\u5ea6\u548c\u7279\u5f81\uff0c\u53ef\u4ee5\u751f\u6210BED\uff08Browser Extensible Data\uff09\u6587\u4ef6\uff0c\u7528\u4e8e\u8868\u793a\u57fa\u56e0\u7ec4\u533a\u57df\u7684\u6ce8\u91ca\u3001\u67d3\u8272\u4f53\u7ed3\u6784\u53d8\u5f02\u7b49\u4fe1\u606f\u3002<o:p><\/o:p><\/p>\n<p><br 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\/><\/p>\n<p>4.\u4e3a\u4e86\u5feb\u901f\u8bbf\u95ee\u548c\u68c0\u7d22BAM\u6587\u4ef6\u4e2d\u7684\u6bd4\u5bf9\u7ed3\u679c\uff0c\u53ef\u4ee5\u5bf9BAM\u6587\u4ef6\u8fdb\u884c\u7d22\u5f15\u6784\u5efa\u3002\u7d22\u5f15\u6587\u4ef6\u63d0\u4f9b\u4e86\u4e00\u79cd\u5feb\u901f\u67e5\u8be2\u7279\u5b9a\u533a\u57df\u7684\u673a\u5236\uff0c\u907f\u514d\u4e86\u9700\u8981\u9010\u884c\u626b\u63cf\u6574\u4e2aBAM\u6587\u4ef6\u7684\u65f6\u95f4\u6d88\u8017\u3002\u76ee\u524d\u5e38\u89c1\u7684BAM\u7d22\u5f15\u6587\u4ef6\u683c\u5f0f\u6709.BAI\uff08Binary Alignment\/Map Index\uff09\u548c.CSI\uff08Coordinate-sorted Binary Index\uff09\u4e24\u79cd\u683c\u5f0f\u3002<\/p>\n<p><o:p><\/o:p><\/p>\n<p>\u4e3a\u4e86\u65b9\u4fbf\u5927\u5bb6\u8bb0\u5fc6\uff0c\u5c0f\u82b1\u81ea\u5236\u4e00\u5f20\u601d\u7ef4\u5bfc\u56fe\uff0c\u6b22\u8fce\u5927\u5bb6\u6536\u85cf\u54e6~<o:p><\/o:p><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.4785714285714286\" 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lang=\"EN-US\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 98%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;border-style: solid;border-width: 2px;border-color: rgb(160, 160, 160);padding: 27px;\">\n<section style=\"text-align: justify;line-height: 1.6;letter-spacing: 0px;padding-right: 6px;padding-left: 6px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<p><br \/><\/p>\n<p>\u5982\u679c\u5c0f\u4f19\u4f34\u4eec\u5e73\u65f6\u5728\u751f\u4fe1\u5206\u6790\u7684\u64cd\u4f5c\u8fc7\u7a0b\u4e2d\u9047\u5230\u56f0\u96be\uff0c\u6b22\u8fce\u5927\u5bb6\u4f7f\u7528\u5c0f\u82b1\u5f00\u53d1\u7684\u751f\u4fe1\u5de5\u5177\u5e73\u53f0http:\/\/www.biocloudservice.com\/home.html\uff0c\u5927\u5bb6\u5728\u65b0\u63a5\u89e6\u4e00\u4e2a\u77e5\u8bc6\u7684\u65f6\u5019\uff0c\u4e0e\u5176\u5148\u82b1\u8d39\u5927\u91cf\u65f6\u95f4\u6b7b\u5543\u77e5\u8bc6\u70b9\uff0c\u4e0d\u5982\u5148\u5229\u7528\u597d\u5de5\u5177\u5148\u81ea\u5df1\u4e0a\u624b\u63a5\u89e6\u6d41\u7a0b\uff0c\u5728\u8dd1\u5b8c\u4e00\u904d\u5168\u6d41\u7a0b\u540e\u518d\u8fd4\u56de\u53bb\u7406\u89e3\u77e5\u8bc6\u70b9\uff0c\u76f8\u4fe1\u53ef\u4ee5\u66f4\u597d\u66f4\u5feb\u5730\u7406\u89e3\uff0c\u8fbe\u5230\u4e8b\u534a\u529f\u500d\u7684\u6548\u679c\uff01<o:p><\/o:p><\/p>\n<p><br 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{"id":52493,"date":"2024-06-16T11:32:35","date_gmt":"2024-06-16T03:32:35","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=52493"},"modified":"2024-06-16T11:32:35","modified_gmt":"2024-06-16T03:32:35","slug":"dna%e7%94%9f%e4%bf%a1%e5%88%86%e6%9e%90%ef%bc%88%e4%b8%80%ef%bc%89%ef%bc%9a%e8%90%8c%e6%96%b0%e5%bf%85%e7%9c%8b%ef%bc%81%e4%b8%80%e5%88%86%e9%92%9f%e5%ad%a6%e4%bc%9a%e5%a6%82%e4%bd%95%e5%a4%84","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=52493","title":{"rendered":"DNA\u751f\u4fe1\u5206\u6790\uff08\u4e00\uff09\uff1a\u840c\u65b0\u5fc5\u770b\uff01\u4e00\u5206\u949f\u5b66\u4f1a\u5982\u4f55\u5904\u7406FASTQ\u6587\u4ef6"},"content":{"rendered":"

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auto;height: auto;overflow: hidden;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<h1><\/h1>\n<h1><span style=\"color: rgb(2, 30, 170);\"><strong>\u4e00\u5206\u949f\u5b66\u4f1a\u5982\u4f55\u5904\u7406FASTQ\u6587\u4ef6<\/strong><\/span><o:p><\/o:p><\/h1>\n<p><span style=\"color: rgb(2, 30, 170);\"><strong><br \/><\/strong><\/span><\/p>\n<p>\u5728\u524d\u9762\u7684\u6587\u7ae0\u4e2d\uff0c\u5c0f\u82b1\u5411\u5927\u5bb6\u5206\u4eab\u4e86\u5355\u7ec6\u80de\u6d4b\u5e8f\u7684\u6d41\u7a0b\uff0c\u60f3\u5fc5\u5c0f\u4f19\u4f34\u4eec\u4e5f\u6210\u529f\u7684\u5b66\u4f1a\u4e86\u6e7f\u5b9e\u9a8c\u7684\u6b65\u9aa4\u5427\u3002\u4f46\u662f\u6e7f\u5b9e\u9a8c\u7684\u7ed3\u675f\u624d\u662f\u6574\u4e2a\u751f\u4fe1\u9879\u76ee\u7684\u5f00\u59cb\uff0c\u9762\u5bf9\u7740\u6d4b\u5e8f\u4eea\u4e0b\u673a\u7684\u539f\u59cb\u6570\u636e\uff0c\u5f88\u591a\u5c0f\u4f19\u4f34\u53ef\u80fd\u611f\u89c9\u65e0\u4ece\u4e0b\u624b\uff0c\u4e0d\u77e5\u9053\u600e\u4e48\u5bf9\u6570\u636e\u8fdb\u884c\u5206\u6790\u3002\u8fd9\u4e00\u6b21\u5c0f\u82b1\u5c31\u8ddf\u5927\u5bb6\u5206\u4eab\u4e00\u4e0b\u600e\u4e48\u9605\u8bfb\u4ece\u6d4b\u5e8f\u4eea\u5f97\u5230\u7684FASTQ\u6587\u4ef6\uff0c\u5927\u5bb6\u5feb\u6765\u770b\u770b\u5427\uff01<o:p><\/o:p><\/p>\n<h2><span style=\"color: rgb(2, 30, 170);\"><strong>\u4ec0\u4e48\u662fFASTQ\u6587\u4ef6\uff1f<\/strong><\/span><o:p><\/o:p><\/h2>\n<p><span style=\"color: rgb(2, 30, 170);\"><strong><br \/><\/strong><\/span><\/p>\n<p>FASTQ\uff08Fast Quality\uff09\u6587\u4ef6\u662f\u4e00\u79cd\u5e38\u7528\u7684\u5b58\u50a8\u6d4b\u5e8f\u6570\u636e\u7684\u6587\u672c\u683c\u5f0f\uff0c\u6587\u4ef6\u540e\u7f00\u901a\u5e38\u4e3a.fastq\u6216.fq\u3002\u5b83\u901a\u5e38\u7528\u4e8e\u5b58\u50a8\u9ad8\u901a\u91cf\u6d4b\u5e8f\uff08\u5982DNA\u6d4b\u5e8f\u6216RNA\u6d4b\u5e8f\uff09\u5f97\u5230\u7684\u539f\u59cb\u6d4b\u5e8f\u7247\u6bb5\uff08reads\uff09\u7684\u5e8f\u5217\u548c\u5bf9\u5e94\u7684\u8d28\u91cf\u4fe1\u606f\u3002<o:p><\/o:p><\/p>\n<p>FASTQ\u6587\u4ef6\u7684\u6bcf\u4e2a\u6d4b\u5e8f\u7247\u6bb5\u901a\u5e38\u7531\u56db\u884c\u7ec4\u6210\uff0c\u5177\u4f53\u683c\u5f0f\u5982\u4e0b\uff1a<o:p><\/o:p><\/p>\n<p>1.\u7b2c\u4e00\u884c\u4ee5\u7b26\u53f7 “@” \u5f00\u5934\uff0c\u540e\u63a5\u4e00\u4e2a\u552f\u4e00\u7684\u6807\u8bc6\u7b26\uff0c\u8868\u793a\u8be5\u5e8f\u5217\u7684\u540d\u79f0\u6216\u7f16\u53f7\u3002<o:p><\/o:p><\/p>\n<p>2.\u7b2c\u4e8c\u884c\u662f\u5bf9\u5e94\u6d4b\u5e8f\u7247\u6bb5\u7684\u78b1\u57fa\u5e8f\u5217\uff0c\u7531A\u3001T\u3001C\u3001G\u7b49\u78b1\u57fa\u5b57\u7b26\u7ec4\u6210\u3002<o:p><\/o:p><\/p>\n<p>3.\u7b2c\u4e09\u884c\u4ee5\u7b26\u53f7 “+” 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rgb(250, 250, 250);color: rgb(51, 51, 51);letter-spacing: 0.034em;\"><\/span><\/p>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9392857142857143\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_aLgbaJIa1AJB3iaIialzKztPicg6NA-6.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibialhsBGNiaEXCOuwBz71GSaL5eic49iaLgbaJIa1AJB3iaIialzKztPicg6NA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"560\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<p>\u8fd9\u4e2aFASTQ\u6587\u4ef6\u662f\u5c0f\u679c\u5728\u4e00\u6b21DNA\u6d4b\u5e8f\u540e\u4ece\u6d4b\u5e8f\u4eea\u76f4\u63a5\u5f97\u5230\u7684\u4e0b\u673a\u6570\u636e\u3002\u4ee5\u8fd9\u4e2a\u6587\u4ef6\u4e3a\u4f8b\uff0c\u5c0f\u4f19\u4f34\u4eec\u662f\u4e0d\u662f\u5df2\u7ecf\u7406\u89e3\u4e86\u6bcf\u4e2a\u7247\u6bb5\u7684\u524d\u4e09\u884c\u5462\uff1f\u63a5\u4e0b\u6765\u5c0f\u82b1\u5c31\u8be6\u7ec6\u7ed9\u5927\u5bb6\u89e3\u91ca\u4e00\u4e0b\u7b2c\u56db\u884c\u8d28\u91cf\u503c\u7684\u9605\u8bfb\u65b9\u6cd5\u3002<\/p>\n<p><span lang=\"EN-US\"><o:p><br \/><\/o:p><\/span><\/p>\n<p><span lang=\"EN-US\" style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:\nCambria;mso-ascii-theme-font:minor-latin;mso-fareast-theme-font:minor-fareast;mso-hansi-font-family:Cambria;mso-hansi-theme-font:minor-latin;\"><br \/><\/span><\/p>\n<h2><\/h2>\n<h2><span style=\"color: rgb(2, 30, 170);\"><strong>\u4ec0\u4e48\u662f\u8d28\u91cf\u503cQ\u548c\u8d28\u91cf\u5206\u6570Phred\uff1f<\/strong><\/span><\/h2>\n<p>\u8d28\u91cf\u503cQ\u8868\u793a\u4e86\u6d4b\u5e8f\u7247\u6bb5\u4e2d\u6bcf\u4e2a\u78b1\u57fa\u7684\u53ef\u4fe1\u5ea6\uff0c\u78b1\u57fa\u8d28\u91cf\u503cQ\u8ba1\u7b97\u516c\u5f0f\uff1a<\/p>\n<p><span lang=\"EN-US\"><o:p><\/o:p><\/span><\/p>\n<p><span lang=\"EN-US\" style=\"mso-no-proof:yes;\"><\/span><span lang=\"EN-US\"><o:p><\/o:p><\/span><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><br \/><\/section>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3459715639810427\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_Y6rGnZBtyHPNKk5cukEqf23RsoIsAA-6.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibBibxudZckMAw1qXpu3MVj9AiaNY6rGnZBtyHPNKk5cukEqf23RsoIsAA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"211\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p>\u5176\u4e2dp\u4e3a\u78b1\u57fa\u88ab\u8bc6\u522b\u9519\u8bef\u7684\u6982\u7387\u3002\u6839\u636e\u516c\u5f0f\u8ba1\u7b97\u6240\u5f97Q\u503c\u8d8a\u9ad8\uff0c\u4ee3\u8868\u78b1\u57fa\u88ab\u6d4b\u9519\u7684\u6982\u7387\u8d8a\u5c0f\u3002\u4f8b\u5982\u6d4b\u5e8f\u6570\u636e\u4e0b\u673a\u8d28\u91cf\u8bc4\u4ef7\u6307\u6807\u4e2d\uff0cQ20\uff0cQ30\uff0cQ40\u5c31\u5206\u522b\u4ee3\u8868\u6d4b\u5e8f\u9519\u8bef\u7387\u4e3a1%\uff0c0.1%\uff0c0.01%\u3002\u4e0b\u673a\u6570\u636eQ30 >85%\uff0c\u4ee3\u8868\u6240\u6709\u4e0b\u673a\u6570\u636e\u5f53\u4e2d\u6d4b\u5e8f\u9519\u8bef\u7387\u5c0f\u4e8e0.1%\u7684\u78b1\u57fa\u5360\u603b\u78b1\u57fa\u6570\u7684\u6bd4\u4f8b\u8d85\u8fc785%\u3002<o:p><\/o:p><\/p>\n<p>Phred\u8d28\u91cf\u5206\u6570\u662f\u4e00\u4e2a\u8d1f\u6570\uff0c\u4ee3\u8868\u7740\u9519\u8bef\u7684\u6982\u7387\uff0c\u4e00\u822c\u57280\u523041\u4e4b\u95f4\uff08ASCII\u7f16\u7801\u7684\u8303\u56f4\u662f33-73\uff09\uff0c\u8f83\u4f4e\u7684\u8d28\u91cf\u5206\u6570\u8868\u793a\u8f83\u9ad8\u7684\u9519\u8bef\u6982\u7387\u3002<o:p><\/o:p><\/p>\n<p>\u4e3a\u4e86\u5bf9\u78b1\u57fa\u8d28\u91cf\u503c\u8fdb\u884c\u51c6\u786e\u8bb0\u5f55\u5e76\u8282\u7ea6\u5b58\u50a8\uff0c\u91c7\u7528ASCII\u8868\u5bf9\u78b1\u57fa\u8d28\u91cf\u503c\u8fdb\u884c\u8f6c\u6362\u3002\u5bf9\u5e94\u8f6c\u6362\u65b9\u5f0f\u6bd4\u8f83\u5e38\u89c1\u7684\u6709Phred33\u548cPhred64\u4e24\u79cd\u4e0d\u540c\u7684\u8d28\u91cf\u4f53\u7cfb\u3002<o:p><\/o:p><\/p>\n<p>Phred33\uff1a\u4eceASCII\u7801\u8868\u53ef\u6253\u5370\u7684\u5b57\u7b26\u5f00\u59cb\uff0c\u5c06\u8d28\u91cf\u503c0~40\u5bf9\u5e94\u8f6c\u6362\u4e3aASCII\u503c33\u523073\u5bf9\u5e94\u7684\u63a7\u5236\u5b57\u7b26\u201c\uff01\u201d~\u201cI\u201d\uff1a\uff08\u5373\u7528\u201c\u5b9e\u9645\u78b1\u57fa\u8d28\u91cf\u503c+33\u201d\u6240\u5f97\u6570\u503c\u5bf9\u5e94\u7684ASCII\u7801\u8868\u4e2d\u7684\u63a7\u5236\u5b57\u7b26\u4ee3\u8868\u8be5\u78b1\u57fa\u8d28\u91cf\u503c\uff09<o:p><\/o:p><\/p>\n<p>Phred64\uff1a\u7528\u201c\u5b9e\u9645\u78b1\u57fa\u8d28\u91cf\u503c+64\u201d \u6240\u5f97\u6570\u503c\u5bf9\u5e94\u7684ASCII\u7801\u8868\u4e2d\u7684\u63a7\u5236\u5b57\u7b26\u4ee3\u8868\u8be5\u78b1\u57fa\u8d28\u91cf\u503c\u3002<o:p><\/o:p><\/p>\n<p>\u5176\u4ed6\u8d28\u91cf\u503c\u4f53\u7cfb\u5982Solexa+64\u7b49\u7684\u8d28\u91cf\u503c\u4e0e\u63a7\u5236\u5b57\u7b26\u7684\u5bf9\u5e94\u5173\u7cfb\u89c1\u4ee5\u4e0b\u56fe\u793a\u3002<\/p>\n<p><span lang=\"EN-US\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<\/section>\n<p><span style=\"display: none;line-height: 0px;\">\u200d<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.425\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_pWEpNMQrct5pkwwqBqpmuUXNibnVgg-6.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibEiaWqBM9iaCQvpck6UniaSrVnftnpWEpNMQrct5pkwwqBqpmuUXNibnVgg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"560\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><br 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170);\"><strong>FASTQ\u6587\u4ef6\u540e\u7eed\u5982\u4f55\u5904\u7406\uff1f<\/strong><\/span><o:p><\/o:p><\/h2>\n<p>\u521d\u5165\u751f\u4fe1\u7684\u5c0f\u4f19\u4f34\u4eec\u53ef\u80fd\u542c\u8bf4\u8fc7FASTA\u6587\u4ef6\uff08.fasta\/.fa)\uff0c\u4e0eFASTQ\u540d\u79f0\u6709\u516b\u6210\u751a\u81f3\u4e5d\u6210\u7684\u76f8\u4f3c\uff0c\u53ef\u80fd\u5f88\u591a\u5c0f\u4f19\u4f34\u90fd\u4e0d\u592a\u80fd\u5206\u6e05\u4ed6\u4eec\uff0c\u73b0\u5728\u5c31\u8ba9\u5c0f\u679c\u7528\u201c\u7167\u5996\u955c\u201d\u63ed\u5f00FASTQ\u6587\u4ef6\u7684\u670b\u53cb\u4eec\u7684\u795e\u79d8\u9762\u7eb1\u5427\uff1a<o:p><\/o:p><\/p>\n<p>\u4e3e\u4e00\u4e2aFASTA\u6587\u4ef6\u7684\u4f8b\u5b50\uff1a<\/p>\n<p><o:p><\/o:p><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5517857142857143\" data-s=\"300,640\" 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style=\"letter-spacing: 0.034em;\"><\/span><o:p><\/o:p><\/p>\n<p>1.\u5bf9\u6bd4\u5bf9\u7ed3\u679c\u8fdb\u884c\u53d8\u5f02\u68c0\u6d4b\u65f6\uff0c\u53ef\u4ee5\u5c06SAM\/BAM\u6587\u4ef6\u4e0e\u53c2\u8003\u57fa\u56e0\u7ec4\u8fdb\u884c\u6bd4\u8f83\uff0c\u8bc6\u522b\u51fa\u6d4b\u5e8f\u6837\u672c\u4e2d\u4e0e\u53c2\u8003\u57fa\u56e0\u7ec4\u4e0d\u540c\u7684\u53d8\u5f02\u4f4d\u70b9\uff0c\u5e76\u751f\u6210Variant Call Format\uff08VCF\uff09\u6587\u4ef6\u3002<o:p><\/o:p><\/p>\n<p><br \/><\/p>\n<p>2.\u6839\u636eSAM\/BAM\u6587\u4ef6\u7684\u8986\u76d6\u7a0b\u5ea6\u548c\u7279\u5f81\uff0c\u53ef\u4ee5\u751f\u6210BED\uff08Browser Extensible Data\uff09\u6587\u4ef6\uff0c\u7528\u4e8e\u8868\u793a\u57fa\u56e0\u7ec4\u533a\u57df\u7684\u6ce8\u91ca\u3001\u67d3\u8272\u4f53\u7ed3\u6784\u53d8\u5f02\u7b49\u4fe1\u606f\u3002<o:p><\/o:p><\/p>\n<p><br 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\/><\/p>\n<p>4.\u4e3a\u4e86\u5feb\u901f\u8bbf\u95ee\u548c\u68c0\u7d22BAM\u6587\u4ef6\u4e2d\u7684\u6bd4\u5bf9\u7ed3\u679c\uff0c\u53ef\u4ee5\u5bf9BAM\u6587\u4ef6\u8fdb\u884c\u7d22\u5f15\u6784\u5efa\u3002\u7d22\u5f15\u6587\u4ef6\u63d0\u4f9b\u4e86\u4e00\u79cd\u5feb\u901f\u67e5\u8be2\u7279\u5b9a\u533a\u57df\u7684\u673a\u5236\uff0c\u907f\u514d\u4e86\u9700\u8981\u9010\u884c\u626b\u63cf\u6574\u4e2aBAM\u6587\u4ef6\u7684\u65f6\u95f4\u6d88\u8017\u3002\u76ee\u524d\u5e38\u89c1\u7684BAM\u7d22\u5f15\u6587\u4ef6\u683c\u5f0f\u6709.BAI\uff08Binary Alignment\/Map Index\uff09\u548c.CSI\uff08Coordinate-sorted Binary Index\uff09\u4e24\u79cd\u683c\u5f0f\u3002<\/p>\n<p><o:p><\/o:p><\/p>\n<p>\u4e3a\u4e86\u65b9\u4fbf\u5927\u5bb6\u8bb0\u5fc6\uff0c\u5c0f\u82b1\u81ea\u5236\u4e00\u5f20\u601d\u7ef4\u5bfc\u56fe\uff0c\u6b22\u8fce\u5927\u5bb6\u6536\u85cf\u54e6~<o:p><\/o:p><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.4785714285714286\" 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