<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 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#e7e7eb;}@supports(-webkit-overflow-scrolling:touch){.rich_media_title{font-weight:700}}.rich_media_meta{display:inline-block;vertical-align:middle;padding:0 0 10px 0;font-size:15px;-webkit-tap-highlight-color:rgba(0,0,0,0)}.rich_media_meta.icon_appmsg_tag{margin-right:0px}.rich_media_meta.meta_tag_text{margin-right:0}.rich_media_meta_list em{font-style:normal}.rich_media_meta_text{color:#a5a5a5;}p{margin:0;}.msgBox{margin-top:20px;padding-top:20px;padding-left:50px;overflow:hidden;border-top:2px dashed #09a2ff;}.msg{padding-top:7px;clear:both;}.msgBody{float:right;width:100%;margin-left:55px;padding-bottom:15px;border-bottom:1px dashed #e0e0e0;}.userHeadImg{float:left;margin-left:-50px;}.userHeadImg img{width:40px;height:40px;margin-right:10px;border-radius:3px;}.userName{color:#888888;line-height:24px;font-size:14px;margin:5px 0 5px 0;height:24px;}.replyBody,.autherBody{color:#565656;font-size:15px;}.replyIcon{border-left:4px solid #33ab01;margin-right:5px;}.ad{text-decoration:none;color:#d6d4d4;font-size:12px;}.msgBodyReply{padding-top:5px;}.userName span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation 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0;align-items:center}.flex_bd{padding-left:14px;}.account_nickname{display:inline-block;vertical-align:middle;line-height:1.2;color:#576b95;font-size:14px}.account_desc{overflow:hidden;text-overflow:ellipsis;display:-webkit-box;-webkit-box-orient:vertical;-webkit-line-clamp:1;color:rgba(0,0,0,0.3);font-size:14px;line-height:1.2;padding-top:.4em}.msg_source_url{text-align:left;word-break:break-all;margin-top:20px;}.msg_source_url a{padding-right:10px;}.msg_source_url .url_text{color:#a8a8a8;}.video-desc{font-size:14px;margin-top:15px;color:#6c6c6c;}.msg_source_url{text-align:left;}.original_primary_card_tips{color:rgba(0,0,0,0.3);line-height:1.4;font-size:15px;}.weui-flex__item{margin-bottom:20px;padding:20px 16px;margin-top:16px;line-height:1.4;align-items:center;background-color:#f7f7f7;border-radius:8px;position:relative;}.original_primary_desc{color:rgba(0,0,0,0.5);font-size:14px;padding-top:4px;width:auto;overflow:hidden;text-overflow:ellipsis;}.msgBodyReplyList{border-top:1px solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"SCP\u63a2\u7d22\u5355\u7ec6\u80de\u5b87\u5b99\u7684\u9b54\u6cd5\u5de5\u5177\uff0c\u52a0\u901f\u751f\u7269\u4fe1\u606f\u5b66\u7814\u7a76\",\"time\":\"2023-09-20 19:00:13\",\"timeStamp\":\"1695207613\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247493257&idx=1&sn=61d8dd7856912ade9c1240b5e1cf7d24&chksm=cf7f764cf808ff5ae85d0720a187a9330dae13a5ff506c92ad9756c8fa453f83ec26bbcbf927#rd\" target=\"_blank\" rel=\"noopener\">SCP\u63a2\u7d22\u5355\u7ec6\u80de\u5b87\u5b99\u7684\u9b54\u6cd5\u5de5\u5177\uff0c\u52a0\u901f\u751f\u7269\u4fe1\u606f\u5b66\u7814\u7a76<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span id=\"copyright_logo\" class=\"wx_tap_link js_wx_tap_highlight rich_media_meta icon_appmsg_tag appmsg_title_tag weui-wa-hotarea\">\u539f\u521b <\/span><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u679c <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-09-20 19:00:13<\/em><\/div>\n<p><content><\/p>\n<div class=\"topic\">\n<p>\u6536\u5f55\u4e8e\u8bdd\u9898<\/p>\n<p><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2812010014583390210#wechat_redirect\" title=\"R\u4ee3\u7801\" target=\"_blank\" rel=\"noopener\">#R\u4ee3\u7801<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=3142740974448934918#wechat_redirect\" title=\"\u79d1\u7814\u4f5c\u56fe\" target=\"_blank\" rel=\"noopener\">#\u79d1\u7814\u4f5c\u56fe<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=3142533467331887107#wechat_redirect\" title=\"\u5355\u7ec6\u80de\u6d4b\u5e8f\" target=\"_blank\" rel=\"noopener\">#\u5355\u7ec6\u80de\u6d4b\u5e8f<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2664545260517294084#wechat_redirect\" title=\"\u751f\u4fe1\u5b9e\u64cd\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u5b9e\u64cd<\/a><\/p>\n<\/div>\n<section data-mpa-powered-by=\"yiban.io\">\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section powered-by=\"xiumi.us\">\n<section>\n<p><span style=\"font-size: 16px;\">\u5728\u4e4b\u524d\uff0c\u5c0f\u679c\u4e3a\u5927\u5bb6\u4ecb\u7ecd\u4e86popsicleR\u8fd9\u4e2a\u4ee4\u4eba\u60ca\u53f9\u7684R\u5305\uff0c\u5b83\u63d0\u4f9b\u4e86\u4ece\u5355\u7ec6\u80de\u5206\u6790\u8d28\u63a7\u5230\u7ec6\u80de\u6ce8\u91ca\u7684\u6574\u5957\u6d41\u7a0b\u3002\u8fd9\u79cd\u7c7b\u578b\u7684R\u5305\u88ab\u79f0\u4e3asingle-cell pipeline\uff0c\u4e3a\u6211\u4eec\u63d0\u4f9b\u4e86\u4fef\u77b0\u6574\u4e2a\u5355\u7ec6\u80de\u5206\u6790\u6d41\u7a0b\u7684\u4fbf\u6377\u9014\u5f84\uff0c\u8ba9\u6211\u4eec\u80fd\u591f\u7ad9\u5728R\u5305\u7684\u80a9\u8180\u4e0a\uff0c\u8f7b\u677e\u5e94\u5bf9\u5355\u7ec6\u80de\u6570\u636e\u7684\u6311\u6218\u3002<br \/><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;\">\u800c\u4eca\u5929\uff0c\u5c0f\u679c\u8981\u5411\u5927\u5bb6\u4ecb\u7ecd\u4e00\u6b3e\u66f4\u8fdb\u9636\u7684R\u5305\u2014\u2014SCP\uff08single-cell pipeline\uff09\uff0c\u5b83\u4e0d\u4ec5\u63d0\u4f9b\u4e86\u9ad8\u7ea7\u7684\u5355\u7ec6\u80de\u5206\u6790\u529f\u80fd\uff0c\u4f8b\u5982\u62df\u65f6\u5e8f\u5206\u6790\u3001\u901f\u7387\u5206\u6790\u7b49\uff0c\u8fd8\u62e5\u6709\u5404\u79cd\u7f8e\u89c2\u5927\u6c14\u7684\u6570\u636e\u53ef\u89c6\u5316\u56fe\u3002\u8fd9\u6b3eR\u5305\u662f\u7531\u5357\u4eac\u533b\u79d1\u5927\u5b66\u751f\u6b96\u533b\u5b66\u535a\u58eb\u4eb2\u81ea\u7f16\u5199\uff0c\u53ef\u89c1\u5176\u4e13\u4e1a\u6027\u4e0e\u5b9e\u7528\u6027\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;\">SCP R\u5305\u662f\u4e00\u5957\u5f3a\u5927\u7684\u5355\u7ec6\u80de\u6570\u636e\u5904\u7406\u548c\u5206\u6790\u5de5\u5177\uff0c\u63d0\u4f9b\u4e86\u5168\u9762\u7684\u89e3\u51b3\u65b9\u6848\u3002\u5176\u529f\u80fd\u4e4b\u5f3a\u5927\uff0c\u6db5\u76d6\u4e86\u4f17\u591a\u751f\u7269\u4fe1\u606f\u5b66\u5e38\u7528\u5de5\u5177\u548c\u51fd\u6570\uff0c\u65e8\u5728\u7b80\u5316\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u7684\u590d\u6742\u6027\uff0c\u4f7f\u7814\u7a76\u8005\u80fd\u591f\u66f4\u4e13\u6ce8\u4e8e\u79d1\u5b66\u63a2\u7d22\uff0c\u800c\u65e0\u9700\u82b1\u8d39\u8fc7\u591a\u65f6\u95f4\u5728\u7e41\u7410\u7684\u6570\u636e\u5904\u7406\u4e0a\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;\">\u5bf9\u4e8e\u521d\u5b66\u8005\u800c\u8a00\uff0c\u5b66\u4e60\u8fd9\u6837\u4e00\u4e2a\u529f\u80fd\u5f3a\u5927\uff0c\u6c47\u96c6\u4e86\u5927\u91cf\u51fd\u6570\u7684R\u5305\uff0c\u6700\u521d\u7684\u96be\u70b9\u5f80\u5f80\u5728\u4e8e\u5982\u4f55\u6210\u529f\u5b89\u88c5\u3002\u56e0\u6b64\uff0c\u63a5\u4e0b\u6765\u5c0f\u679c\u5c06\u4e3a\u5927\u5bb6\u5c55\u793a\u5982\u4f55\u5728\u670d\u52a1\u5668\u548c\u672c\u5730\u7535\u8111\u4e24\u4e2a\u4e0d\u540c\u7684\u7ec8\u7aef\u4e0a\u987a\u5229\u4e0b\u8f7dSCP\uff0c\u8ba9\u5927\u5bb6\u8f7b\u677e\u8fc8\u5165\u5355\u7ec6\u80de\u6570\u636e\u7684\u7cbe\u5f69\u4e16\u754c\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;\">\u6e29\u99a8\u63d0\u9192\uff1aR \u7248\u672c >= 4.1.0\u54e6<\/span><span style=\"mso-spacerun:'yes';font-family:Cambria;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:12.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<h1 style=\"text-align: center;\"><em><strong><span style=\"font-family: Calibri;font-size: 16pt;\">Linux<span style=\"font-family:\u5b8b\u4f53;\">\u7ec8\u7aef<\/span><\/span><\/strong><\/em><strong><span style=\"font-family: Calibri;color: rgb(79, 129, 189);font-size: 16pt;\"><o:p><\/o:p><\/span><\/strong><\/h1>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5728\u670d\u52a1\u5668\u4e2d\u8fdb\u884c\u5b89\u88c5\uff0c\u9996\u5148\u6211\u4eec\u5f97\u65b0\u5efa\u4e00\u4e2aconda\u7684\u865a\u62df\u73af\u5883<\/span><em style=\"letter-spacing: 0.034em;\"><\/em><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"sql\"><code><span class=\"code-snippet_outer\"># \u521b\u5efa\u65b0\u7684\u865a\u62df\u73af\u5883<\/span><\/code><code><span class=\"code-snippet_outer\">conda create -n SCP_env r=4.1.3<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u8fdb\u5165\u521b\u5efa\u7684\u65b0\u73af\u5883\u4e2d<\/span><\/code><code><span class=\"code-snippet_outer\">conda activate SCP_env<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u968f\u540e\u8fdb\u884cconda\u5b98\u7f51\u5bf9\u6240\u9700\u8981\u7684R\u5305\u8fdb\u884c\u4e0b\u8f7d<\/span><\/code><code><span class=\"code-snippet_outer\">## https:\/\/anaconda.org\/anaconda\/conda<\/span><\/code><code><span class=\"code-snippet_outer\">conda install -c conda-forge r-devtools # \u6211\u4eec\u5148\u9700\u8981\u5b89\u88c5devtools\uff0c\u4e0b\u8f7d\u5728GitHub\u4e0a\u7684SCP\u5305<\/span><\/code><code><span class=\"code-snippet_outer\"># \u4e5f\u53ef\u4ee5\u9009\u62e9\u5728R\u73af\u5883\u4e2d\u8fdb\u884c\u4e0b\u8f7d\uff0c\u5c0f\u679c\u6bd4\u8f83\u504f\u5411\u4e8econda\u5b89\u88c5\u54e6~ \u8fd9\u4e2a\u9009\u62e9\u5e76\u6ca1\u6709\u5bf9\u9519\u4e4b\u5206\uff0c\u54ea\u79cd\u65b9\u6cd5\u66f4\u9002\u5408\u4f60\uff0c\u4e0b\u8f7d\u901f\u5ea6\u66f4\u5feb\uff0c\u4f7f\u7528\u4fbf\u662f\u5566\uff01\u4ee5\u7ed3\u679c\u4e3a\u5bfc\u5411\uff01\uff01<\/span><\/code><code><span class=\"code-snippet_outer\">if (!require(\"devtools\", quietly = TRUE)) {<\/span><\/code><code><span class=\"code-snippet_outer\"> install.packages(\"devtools\")<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">options(timeout = 999) # \u5728\u4f7f\u7528devtools::install_github()\u51fd\u6570\u65f6\uff0c\u90fd\u8981\u8bbe\u7f6e\u597d\u8fd9\u4e2a\u53c2\u6570\u54e6\uff0c\u5b83\u53ef\u4ee5\u5e2e\u52a9\u6211\u4eec\u987a\u5229\u7684\u4eceGitHub\u4e0a\u4e0b\u8f7dR\u5305<\/span><\/code><code><span class=\"code-snippet_outer\">devtools::install_github(\"zhanghao-njmu\/SCP\")<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u5728\u5b89\u88c5SCP\u7684\u8fc7\u7a0b\u4e2d\u53ef\u80fd\u4f1a\u51fa\u73b0\u67d0\u4e00\u5305\u65e0\u6cd5\u5b89\u88c5\u7684\u60c5\u51b5\uff0c\u6211\u4eec\u53ef\u4ee5\u4f7f\u7528conda\u8fdb\u884c\u5b89\u88c5\uff0c\u5b89\u88c5\u65b9\u6cd5\u4e0e\u5b89\u88c5devtools\u4e00\u81f4\uff0c\u5728\u8fd9\u5c0f\u679c\u5c31\u4e0d\u8d58\u8ff0\u5566\u3002<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<h1 style=\"text-align: center;\"><em><strong><span style=\"font-family: Calibri;font-size: 16pt;\">Windows<span style=\"font-family:\u5b8b\u4f53;\">\u7ec8\u7aef<\/span><\/span><\/strong><\/em><\/h1>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\">env_dir <- \"D:\/SCP_env\/\"<\/span><\/code><code><span class=\"code-snippet_outer\">dir.create(env_dir,recursive = TRUE)<\/span><\/code><code><span class=\"code-snippet_outer\">setwd(env_dir)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u4f7f\u7528\u5c0f\u679c\u66fe\u7ecf\u6559\u8fc7\u5927\u5bb6\u7684renv\u5305\uff0c\u5728\u672c\u5730\u521b\u5efa\u4e00\u4e2a\u65b0\u7684\u73af\u5883<\/span><\/code><code><span class=\"code-snippet_outer\">if(!require(\"renv\")) install.packages(renv)<\/span><\/code><code><span class=\"code-snippet_outer\">renv::init(project = env_dir)<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 14px;font-family: \u5b8b\u4f53;\"><br \/><\/span><\/p>\n<p><span style=\"font-size: 14px;\"><span style=\"font-size: 14px;font-family: Cambria;\"><span style=\"font-family: \u5b8b\u4f53;\">\u5728\u6210\u529f\u521b\u5efa\u73af\u5883\u540e\uff0c\u6211\u4eec\u53ef\u4ee5\u770b\u5230\u8fd9\u6837\u7684\u8f93\u51fa<\/span> <\/span><span style=\"font-family: Cambria;font-size: 12pt;\"><o:p><\/o:p><\/span><\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: \u5b8b\u4f53;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.175\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_TsqCGM3DibsnOBumZRvUkVgA7780uw-2.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4ETD3aPH8PDqwphkIicXu9G1wK2ibTsqCGM3DibsnOBumZRvUkVgA7780uw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"720\" style=\"\" \/><\/p>\n<p><br \/><\/p>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5728\u8fdb\u5165\u73af\u5883\u4e2d\uff0c\u6211\u4eec\u4fbf\u53ef\u4ee5\u5f00\u59cb\u5b89\u88c5R\u5305\uff0c\u548cLinux\u7ec8\u7aef\u4e00\u6837\uff0c\u6211\u4eec\u9996\u5148\u9700\u8981\u4e0b\u8f7d\u7684\u662fdevtools\u548cBiocManager\u4e24\u4e2aR\u5305\uff0c\u5bf9\u540e\u7eed\u7684R\u5305\u8fdb\u884c\u5b89\u88c5\u64cd\u4f5c\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:Cambria;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:12.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\">if (!require(\"devtools\", quietly = TRUE)) {<\/span><\/code><code><span class=\"code-snippet_outer\"> install.packages(\"devtools\")<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">options(timeout = 999)<\/span><\/code><code><span class=\"code-snippet_outer\">devtools::install_github(\"zhanghao-njmu\/SCP\")<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5728\u5b89\u88c5\u7684\u8fc7\u7a0b\u4e2d\uff0c\u6211\u4eec\u53ef\u80fd\u4f1a\u9047\u5230\u8fd9\u79cd\u60c5\u51b5<\/span><span style=\"mso-spacerun:'yes';font-family:Cambria;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:12.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2791666666666667\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_gCkgAx3Y0p6TsddSzTUtChpWibFTmA-2.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4E8kicMiaqhPAJgwFgvvJSYsHrCxBgCkgAx3Y0p6TsddSzTUtChpWibFTmA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"720\" style=\"\" \/><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5c0f\u4f19\u4f34\u4eec\uff0c\u9047\u5230\u62a5\u9519\u6211\u4eec\u4e0d\u8981\u5bb3\u6015\u54e6\uff0c\u8fd9\u4e2a\u62a5\u9519\u53ea\u662f\u56e0\u4e3a\u6211\u4eec\u7684\u4f9d\u8d56\u6ca1\u6709\u5b89\u88c5\u6210\u529f\u5462\uff01\u6211\u4eec\u53ea\u9700\u8981\u628a\u62a5\u9519\u4e0a\u7684\u624b\u52a8\u7684\u5b89\u88c5\u597d\u5c31\u53ef\u4ee5\u7ee7\u7eed\u8fd0\u884c\u5566<\/span><span style=\"mso-spacerun:'yes';font-family:Cambria;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:12.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\">if (!require(\"BiocManager\", quietly = TRUE)) {<\/span><\/code><code><span class=\"code-snippet_outer\"> install.packages(\"BiocManager\")<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">BiocManager::install(\"R_names\")<\/span><\/code><code><span class=\"code-snippet_outer\"># \u5982\u679c\u78b0\u4e0a\u7f51\u7edc\u4e0d\u7a33\u5b9a\u4e5f\u53ef\u4ee5\u8fdbbioconductor\u5b98\u7f51(https:\/\/www.bioconductor.org\/)\u4e0b\u8f7d\u5b89\u88c5\u5305\u540e\u8fdb\u884c\u672c\u5730\u4e0b\u8f7d\u54e6<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(\".tar.gz_dir\", repos = NULL, type = \"source\")<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u5b89\u88c5\u597dR\u8bed\u8a00\u73af\u5883\u4e0b\u7684R\u5305\u540e\uff0c\u6211\u4eec\u4fbf\u53ef\u4ee5\u8f7d\u5165SCP\u5305\uff0c\u51c6\u5907\u914d\u7f6epython\u73af\u5883\u4e0b\u7684\u5305\u5566\uff0c\u8fd9\u662f\u56e0\u4e3a\u5355\u7ec6\u80de\u5206\u6790\u7684\u8fc7\u7a0b\u4e2d\uff0c\u5982\uff1a\u901f\u7387\u5206\u6790\u7b49\u662f\u9700\u8981python\u73af\u5883\u7684\uff0c\u56e0\u6b64\u6211\u4eec\u5728\u672c\u5730\u4e2d\u4e5f\u9700\u8981\u4e0b\u8f7dconda\uff0c\u914d\u7f6e\u57fa\u7840\u7684python\u73af\u5883\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">options(reticulate.conda_binary = \"D:\/conda\/envs\/SCP_env\")# \u5728\u8fd9\u4e00\u6b65\u6211\u4eec\u9700\u8981\u6307\u5b9a\u597dconda\u7684\u73af\u5883\uff0c\u5207\u8bb0\u5207\u8bb0\uff01\u8def\u5f84\u6700\u540e\u4e0d\u8981\u52a0\u201c\/\u201d\u54e6\uff0c\u4e0d\u7136\u4f1a\u51fa\u73b0\u62a5\u9519<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u5728Windows\u7684\u5b89\u88c5\u8fc7\u7a0b\u4e2d\uff0c\u6211\u4eec\u8fd8\u9700\u8981\u989d\u5916\u5728conda\u4e2d\u5b89\u88c5\u4e00\u4e2a\u5305<\/span><\/code><code><span class=\"code-snippet_outer\">conda install jaxlib-0.1.70-cp38-none-win_amd64.whl<\/span><\/code><code><span class=\"code-snippet_outer\"># \u53ef\u4ee5\u4ece\u8fd9\u4e2a\u7f51\u5740\u4e2d\u4e0b\u8f7d\u9002\u5408\u81ea\u5df1\u7535\u8111\u7684\u5b89\u88c5\u5305\u54e6 https:\/\/whls.blob.core.windows.net\/unstable\/index.html<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u5b89\u88c5python\u5305\u6211\u4eec\u4f7f\u7528\u4e00\u4e0b\u547d\u4ee4<\/span><\/code><code><span class=\"code-snippet_outer\">SCP::PrepareEnv()<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u4f46\u8fd9\u6b65\u662f\u6709\u53ef\u80fd\u62a5\u9519\u7684\uff0c\u90e8\u5206\u5305\u56e0\u4e3a\u7248\u672c\u4e0d\u5bf9\u5e94\u7684\u6216\u662f\u65e0\u6cd5\u6b63\u5e38\u52a0\u8f7d\u7684\uff0c\u6211\u4eec\u90fd\u53ef\u4ee5\u6839\u636e\u62a5\u9519\u5355\u72ec\u7684\u8fdb\u884c\u4e0b\u8f7d\uff0c\u4f7f\u7528\u4ee5\u4e0b\u7684\u547d\u4ee4\uff08\u8fd9\u8fb9\u5c0f\u679c\u4f7f\u7528\u4e86pip\u7684\u4e34\u65f6\u6e05\u534e\u6e90\u8fdb\u884c\u4e0b\u8f7d\u54e6\uff09<\/span><\/code><code><span class=\"code-snippet_outer\">pip install -i https:\/\/pypi.tuna.tsinghua.edu.cn\/simple packages_names<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u65e0\u8bba\u662fLinux\u8fd8\u662fWindows\uff0c\u5f53\u80fd\u6210\u529f\u8fd0\u884c\u4ee5\u4e0b\u4ee3\u7801\u7684\u65f6\u5019\u5c31\u8bc1\u660e\u6211\u4eec\u57fa\u672c\u5df2\u7ecf\u6210\u529f\u5566\uff01\u4e3a\u4ec0\u4e48\u8bf4\u662f\u57fa\u672c\u5462\uff1f\u5c0f\u679c\u5728\u8fd9\u5148\u5356\u4e2a\u5173\u5b50\u54e6<o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"powershell\"><code><span class=\"code-snippet_outer\">library(SCP)<\/span><\/code><code><span class=\"code-snippet_outer\">data(\"pancreas_sub\")<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"kotlin\"><code><span class=\"code-snippet_outer\">pancreas_sub <- RunPAGA(srt = pancreas_sub, group_by = \"SubCellType\", linear_reduction = \"PCA\", nonlinear_reduction = \"UMAP\")<\/span><\/code><code><span class=\"code-snippet_outer\">## 'misc' slot is not converted.<\/span><\/code><code><span class=\"code-snippet_outer\">## 'tools' slot is not converted.<\/span><\/code><code><span class=\"code-snippet_outer\">CellDimPlot(pancreas_sub, group.by = \"SubCellType\", reduction = \"draw_graph_fr\")<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_cBCwnAr64SybN8LL1yNdmEEYnfpzlw-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4EwJIxs2NAiacAq58mWrnkkMOR4cBCwnAr64SybN8LL1yNdmEEYnfpzlw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\" \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code><span class=\"code-snippet_outer\">##\u7ec6\u80de\u805a\u7c7b<\/span><\/code><code><span class=\"code-snippet_outer\">CellDimPlot(<\/span><\/code><code><span class=\"code-snippet_outer\"> srt = pancreas_sub, group.by = c(\"CellType\", \"SubCellType\"),<\/span><\/code><code><span class=\"code-snippet_outer\"> reduction = \"UMAP\", theme_use = \"theme_blank\"<\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_9VeSNJlnwSddDhcaFFyjCYiajPH0sA-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4EPZA0LlQnNOk49vBVYVC9bb2gt9VeSNJlnwSddDhcaFFyjCYiajPH0sA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code><span class=\"code-snippet_outer\">##\u7ec6\u80de\u5468\u671f\u6ce8\u91ca<\/span><\/code><code><span class=\"code-snippet_outer\">CellDimPlot(<\/span><\/code><code><span class=\"code-snippet_outer\"> srt = pancreas_sub, group.by = \"SubCellType\", stat.by = \"Phase\",<\/span><\/code><code><span class=\"code-snippet_outer\"> reduction = \"UMAP\", theme_use = \"theme_blank\"<\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"display: none;line-height: 0px;\">\u200d<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_26pO8ZDnYTYXic5NzpbN0Gic134SUg-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4EJk5ibhLPdvLMak1kCg2pYJwELud26pO8ZDnYTYXic5NzpbN0Gic134SUg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\" \/><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code><span class=\"code-snippet_outer\">##\u7279\u5f81\u6ce8\u91ca<\/span><\/code><code><span class=\"code-snippet_outer\">FeatureDimPlot(<\/span><\/code><code><span class=\"code-snippet_outer\"> srt = pancreas_sub, features = c(\"Sox9\", \"Neurog3\", \"Fev\", \"Rbp4\"),<\/span><\/code><code><span class=\"code-snippet_outer\"> reduction = \"UMAP\", theme_use = \"theme_blank\"<\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_YWp1q7RgDsjMmTbaksPrEgNibiaWNw-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4ELMT78gsALSrzZIRQcKghXokDAeYWp1q7RgDsjMmTbaksPrEgNibiaWNw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\" \/><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">FeatureDimPlot(<\/span><\/code><code><span class=\"code-snippet_outer\">srt = pancreas_sub, features = c(\"Ins1\", \"Gcg\", \"Sst\", \"Ghrl\"),<\/span><\/code><code><span class=\"code-snippet_outer\">compare_features = TRUE, label = TRUE, label_insitu = TRUE,<\/span><\/code><code><span class=\"code-snippet_outer\">reduction = \"UMAP\", theme_use = \"theme_blank\"<\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_7ySWpJDQiakyT0YWEKLTeJk6UbQ52Q-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4EDGFiajR6gHX9J3OQXeUXOrCJMZ7ySWpJDQiakyT0YWEKLTeJk6UbQ52Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\"><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\">##\u7ec6\u80de\u7c7b\u578b\u3001\u767e\u5206\u6bd4\u548c\u7ec6\u80de\u5468\u671f<\/span><\/code><code><span class=\"code-snippet_outer\">ht <- GroupHeatmap(<\/span><\/code><code><span class=\"code-snippet_outer\"> srt = pancreas_sub,<\/span><\/code><code><span class=\"code-snippet_outer\"> features = c(<\/span><\/code><code><span class=\"code-snippet_outer\"> \"Sox9\", \"Anxa2\", # Ductal<\/span><\/code><code><span class=\"code-snippet_outer\"> \"Neurog3\", \"Hes6\", # EPs<\/span><\/code><code><span class=\"code-snippet_outer\"> \"Fev\", \"Neurod1\", # Pre-endocrine<\/span><\/code><code><span class=\"code-snippet_outer\"> \"Rbp4\", \"Pyy\", # Endocrine<\/span><\/code><code><span class=\"code-snippet_outer\"> \"Ins1\", \"Gcg\", \"Sst\", \"Ghrl\" # Beta, Alpha, Delta, Epsilon<\/span><\/code><code><span class=\"code-snippet_outer\"> ),<\/span><\/code><code><span class=\"code-snippet_outer\"> group.by = c(\"CellType\", \"SubCellType\"),<\/span><\/code><code><span class=\"code-snippet_outer\"> heatmap_palette = \"YlOrRd\",<\/span><\/code><code><span class=\"code-snippet_outer\"> cell_annotation = c(\"Phase\", \"G2M_score\", \"Cdh2\"),<\/span><\/code><code><span class=\"code-snippet_outer\"> cell_annotation_palette = c(\"Dark2\", \"Paired\", \"Paired\"),<\/span><\/code><code><span class=\"code-snippet_outer\"> show_row_names = TRUE, row_names_side = \"left\",<\/span><\/code><code><span class=\"code-snippet_outer\"> add_dot = TRUE, add_reticle = TRUE<\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><code><span class=\"code-snippet_outer\">print(ht$plot)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_nq3Fvzia9jiaeRtZmicPc4iaXTxAdg-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4ElHMjug0oqdzvbFq7DxSLT6qHyicJ6nq3Fvzia9jiaeRtZmicPc4iaXTxAdg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\"><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">##\u8d28\u63a7<\/span><\/code><code><span class=\"code-snippet_outer\">## conda install -c bioconda bioconductor-scdblfinder \u4f9d\u8d56\u4e0d\u9002\u914d<\/span><\/code><code><span class=\"code-snippet_outer\"># \u5b89\u88c5\u8def\u5f84<\/span><\/code><code><span class=\"code-snippet_outer\">pancreas_sub <- RunCellQC(srt = pancreas_sub)<\/span><\/code><code><span class=\"code-snippet_outer\">CellDimPlot(srt = pancreas_sub, group.by = \"CellQC\", reduction = \"UMAP\")<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><span style=\"display: none;line-height: 0px;\">\u200d<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_JeVpRcpPiaKQf2Jfx0uKI7bCAPvfIQ-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4Eibw9l344EgqkxqfBxJUrNiaia4d9JeVpRcpPiaKQf2Jfx0uKI7bCAPvfIQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\"><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code><span class=\"code-snippet_outer\">CellStatPlot(srt = pancreas_sub, stat.<span class=\"code-snippet__keyword\">by<\/span> = <span class=\"code-snippet__string\">\"CellQC\"<\/span>, <span class=\"code-snippet__keyword\">group<\/span>.<span class=\"code-snippet__keyword\">by<\/span> = <span class=\"code-snippet__string\">\"CellType\"<\/span>, label = TRUE)<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_SMT7rwMWS4KZiaD9YGSbNgribQW9RA-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4Euv3dNFLGrlPdoE2qCiaMFDqpeAySMT7rwMWS4KZiaD9YGSbNgribQW9RA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\"><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code><span class=\"code-snippet_outer\">CellStatPlot(<\/span><\/code><code><span class=\"code-snippet_outer\"> srt = pancreas_sub,<\/span><\/code><code><span class=\"code-snippet_outer\"> stat.by = c(<\/span><\/code><code><span class=\"code-snippet_outer\"> \"db_qc\", \"outlier_qc\", \"umi_qc\", \"gene_qc\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"mito_qc\", \"ribo_qc\", \"ribo_mito_ratio_qc\", \"species_qc\"<\/span><\/code><code><span class=\"code-snippet_outer\"> ),<\/span><\/code><code><span class=\"code-snippet_outer\"> plot_type = \"upset\", stat_level = \"Fail\"<\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_1REuHP9mfibFtRialZvebibPsMeIYw-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4Ek9Ifvu0PiaTD0FRZ1QQO7sqMnVWN1REuHP9mfibFtRialZvebibPsMeIYw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\"><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code><span class=\"code-snippet_outer\">##\u6807\u51c6\u6d41\u7a0b<\/span><\/code><code><span class=\"code-snippet_outer\">pancreas_sub <- Standard_SCP(srt = pancreas_sub)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">CellDimPlot(<\/span><\/code><code><span class=\"code-snippet_outer\"> srt = pancreas_sub, group.by = c(\"CellType\", \"SubCellType\"),<\/span><\/code><code><span class=\"code-snippet_outer\"> reduction = \"StandardUMAP2D\", theme_use = \"theme_blank\"<\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/12_QIkFYf1Z3SRIibibXLXxV3IxcZh95g-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4E6WprcWld7W73qoS6egpiaaKKfcKQIkFYf1Z3SRIibibXLXxV3IxcZh95g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\"><\/p>\n<section style=\"font-size: 16px;\">\n<section style=\"margin: 10px;text-align: left;justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;padding-right: 10px;padding-left: 10px;border-style: dashed;border-width: 1px;border-color: rgb(62, 62, 62);align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;border-width: 0px 0px 0px 10px;border-style: solid;border-left-color: rgb(144, 215, 236);border-right-color: rgb(46, 172, 109);box-shadow: rgb(0, 0, 0) 0px 0px 0px;border-bottom-left-radius: 0px;\">\n<section style=\"margin-right: 0%;margin-left: 0%;padding-right: 3px;padding-left: 3px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;margin-bottom: -0.65em;margin-top: 0.95em;\">\n<section style=\"width: 100%;\">\n<section style=\"border-top: 1px solid rgb(0, 0, 0);width: 99.9%;float: left;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"width: 6px;height: 6px;margin-top: -3px;border-radius: 100%;float: right;background-color: rgb(46, 172, 109);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"display: inline-block;margin-top: -1em;vertical-align: top;background-color: rgb(255, 255, 255);padding: 3px 10px;color: rgb(0, 154, 68);\">\n<p><strong>\u5f80\u671f\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section style=\"white-space: normal;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491794&idx=1&sn=2b7a4de95169c79202e44813885a8250&chksm=cf7f7017f808f9015689839fe4057fd418bf7b20eb1dfb5a28cd92e56a41b5605e62bfbc16fb&scene=21#wechat_redirect\" textvalue=\"1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4<\/a><br \/><\/section>\n<section style=\"white-space: normal;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491705&idx=1&sn=9ff9b6da28b3a256a99c6697d589be66&chksm=cf7f70bcf808f9aafd1019a0bba3dd3a55e2867dbb9a09203f13c2683adf867094637c405ed1&scene=21#wechat_redirect\" textvalue=\"2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86<\/a><\/section>\n<section style=\"white-space: normal;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491909&idx=1&sn=902e34bcf53e605fba2b457ec6a33e36&chksm=cf7f7180f808f8961542f458a49da14e85d8831449fb65fbf2623d55affc564686a5422f56fd&scene=21#wechat_redirect\" 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data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"SCP\u63a2\u7d22\u5355\u7ec6\u80de\u5b87\u5b99\u7684\u9b54\u6cd5\u5de5\u5177\uff0c\u52a0\u901f\u751f\u7269\u4fe1\u606f\u5b66\u7814\u7a76\",\"time\":\"2023-09-20 19:00:13\",\"timeStamp\":\"1695207613\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247493257&idx=1&sn=61d8dd7856912ade9c1240b5e1cf7d24&chksm=cf7f764cf808ff5ae85d0720a187a9330dae13a5ff506c92ad9756c8fa453f83ec26bbcbf927#rd\" target=\"_blank\" rel=\"noopener\">SCP\u63a2\u7d22\u5355\u7ec6\u80de\u5b87\u5b99\u7684\u9b54\u6cd5\u5de5\u5177\uff0c\u52a0\u901f\u751f\u7269\u4fe1\u606f\u5b66\u7814\u7a76<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span id=\"copyright_logo\" class=\"wx_tap_link js_wx_tap_highlight rich_media_meta icon_appmsg_tag appmsg_title_tag weui-wa-hotarea\">\u539f\u521b <\/span><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u679c <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-09-20 19:00:13<\/em><\/div>\n<p><content><\/p>\n<div class=\"topic\">\n<p>\u6536\u5f55\u4e8e\u8bdd\u9898<\/p>\n<p><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2812010014583390210#wechat_redirect\" title=\"R\u4ee3\u7801\" target=\"_blank\" rel=\"noopener\">#R\u4ee3\u7801<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=3142740974448934918#wechat_redirect\" title=\"\u79d1\u7814\u4f5c\u56fe\" target=\"_blank\" rel=\"noopener\">#\u79d1\u7814\u4f5c\u56fe<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=3142533467331887107#wechat_redirect\" title=\"\u5355\u7ec6\u80de\u6d4b\u5e8f\" target=\"_blank\" rel=\"noopener\">#\u5355\u7ec6\u80de\u6d4b\u5e8f<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2664545260517294084#wechat_redirect\" title=\"\u751f\u4fe1\u5b9e\u64cd\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u5b9e\u64cd<\/a><\/p>\n<\/div>\n<section data-mpa-powered-by=\"yiban.io\">\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section powered-by=\"xiumi.us\">\n<section>\n<p><span style=\"font-size: 16px;\">\u5728\u4e4b\u524d\uff0c\u5c0f\u679c\u4e3a\u5927\u5bb6\u4ecb\u7ecd\u4e86popsicleR\u8fd9\u4e2a\u4ee4\u4eba\u60ca\u53f9\u7684R\u5305\uff0c\u5b83\u63d0\u4f9b\u4e86\u4ece\u5355\u7ec6\u80de\u5206\u6790\u8d28\u63a7\u5230\u7ec6\u80de\u6ce8\u91ca\u7684\u6574\u5957\u6d41\u7a0b\u3002\u8fd9\u79cd\u7c7b\u578b\u7684R\u5305\u88ab\u79f0\u4e3asingle-cell pipeline\uff0c\u4e3a\u6211\u4eec\u63d0\u4f9b\u4e86\u4fef\u77b0\u6574\u4e2a\u5355\u7ec6\u80de\u5206\u6790\u6d41\u7a0b\u7684\u4fbf\u6377\u9014\u5f84\uff0c\u8ba9\u6211\u4eec\u80fd\u591f\u7ad9\u5728R\u5305\u7684\u80a9\u8180\u4e0a\uff0c\u8f7b\u677e\u5e94\u5bf9\u5355\u7ec6\u80de\u6570\u636e\u7684\u6311\u6218\u3002<br \/><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;\">\u800c\u4eca\u5929\uff0c\u5c0f\u679c\u8981\u5411\u5927\u5bb6\u4ecb\u7ecd\u4e00\u6b3e\u66f4\u8fdb\u9636\u7684R\u5305\u2014\u2014SCP\uff08single-cell pipeline\uff09\uff0c\u5b83\u4e0d\u4ec5\u63d0\u4f9b\u4e86\u9ad8\u7ea7\u7684\u5355\u7ec6\u80de\u5206\u6790\u529f\u80fd\uff0c\u4f8b\u5982\u62df\u65f6\u5e8f\u5206\u6790\u3001\u901f\u7387\u5206\u6790\u7b49\uff0c\u8fd8\u62e5\u6709\u5404\u79cd\u7f8e\u89c2\u5927\u6c14\u7684\u6570\u636e\u53ef\u89c6\u5316\u56fe\u3002\u8fd9\u6b3eR\u5305\u662f\u7531\u5357\u4eac\u533b\u79d1\u5927\u5b66\u751f\u6b96\u533b\u5b66\u535a\u58eb\u4eb2\u81ea\u7f16\u5199\uff0c\u53ef\u89c1\u5176\u4e13\u4e1a\u6027\u4e0e\u5b9e\u7528\u6027\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;\">SCP R\u5305\u662f\u4e00\u5957\u5f3a\u5927\u7684\u5355\u7ec6\u80de\u6570\u636e\u5904\u7406\u548c\u5206\u6790\u5de5\u5177\uff0c\u63d0\u4f9b\u4e86\u5168\u9762\u7684\u89e3\u51b3\u65b9\u6848\u3002\u5176\u529f\u80fd\u4e4b\u5f3a\u5927\uff0c\u6db5\u76d6\u4e86\u4f17\u591a\u751f\u7269\u4fe1\u606f\u5b66\u5e38\u7528\u5de5\u5177\u548c\u51fd\u6570\uff0c\u65e8\u5728\u7b80\u5316\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u7684\u590d\u6742\u6027\uff0c\u4f7f\u7814\u7a76\u8005\u80fd\u591f\u66f4\u4e13\u6ce8\u4e8e\u79d1\u5b66\u63a2\u7d22\uff0c\u800c\u65e0\u9700\u82b1\u8d39\u8fc7\u591a\u65f6\u95f4\u5728\u7e41\u7410\u7684\u6570\u636e\u5904\u7406\u4e0a\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;\">\u5bf9\u4e8e\u521d\u5b66\u8005\u800c\u8a00\uff0c\u5b66\u4e60\u8fd9\u6837\u4e00\u4e2a\u529f\u80fd\u5f3a\u5927\uff0c\u6c47\u96c6\u4e86\u5927\u91cf\u51fd\u6570\u7684R\u5305\uff0c\u6700\u521d\u7684\u96be\u70b9\u5f80\u5f80\u5728\u4e8e\u5982\u4f55\u6210\u529f\u5b89\u88c5\u3002\u56e0\u6b64\uff0c\u63a5\u4e0b\u6765\u5c0f\u679c\u5c06\u4e3a\u5927\u5bb6\u5c55\u793a\u5982\u4f55\u5728\u670d\u52a1\u5668\u548c\u672c\u5730\u7535\u8111\u4e24\u4e2a\u4e0d\u540c\u7684\u7ec8\u7aef\u4e0a\u987a\u5229\u4e0b\u8f7dSCP\uff0c\u8ba9\u5927\u5bb6\u8f7b\u677e\u8fc8\u5165\u5355\u7ec6\u80de\u6570\u636e\u7684\u7cbe\u5f69\u4e16\u754c\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;\">\u6e29\u99a8\u63d0\u9192\uff1aR \u7248\u672c >= 4.1.0\u54e6<\/span><span style=\"mso-spacerun:'yes';font-family:Cambria;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:12.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<h1 style=\"text-align: center;\"><em><strong><span style=\"font-family: Calibri;font-size: 16pt;\">Linux<span style=\"font-family:\u5b8b\u4f53;\">\u7ec8\u7aef<\/span><\/span><\/strong><\/em><strong><span style=\"font-family: Calibri;color: rgb(79, 129, 189);font-size: 16pt;\"><o:p><\/o:p><\/span><\/strong><\/h1>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5728\u670d\u52a1\u5668\u4e2d\u8fdb\u884c\u5b89\u88c5\uff0c\u9996\u5148\u6211\u4eec\u5f97\u65b0\u5efa\u4e00\u4e2aconda\u7684\u865a\u62df\u73af\u5883<\/span><em style=\"letter-spacing: 0.034em;\"><\/em><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"sql\"><code><span class=\"code-snippet_outer\"># \u521b\u5efa\u65b0\u7684\u865a\u62df\u73af\u5883<\/span><\/code><code><span class=\"code-snippet_outer\">conda create -n SCP_env r=4.1.3<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u8fdb\u5165\u521b\u5efa\u7684\u65b0\u73af\u5883\u4e2d<\/span><\/code><code><span class=\"code-snippet_outer\">conda activate SCP_env<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u968f\u540e\u8fdb\u884cconda\u5b98\u7f51\u5bf9\u6240\u9700\u8981\u7684R\u5305\u8fdb\u884c\u4e0b\u8f7d<\/span><\/code><code><span class=\"code-snippet_outer\">## https:\/\/anaconda.org\/anaconda\/conda<\/span><\/code><code><span class=\"code-snippet_outer\">conda install -c conda-forge r-devtools # \u6211\u4eec\u5148\u9700\u8981\u5b89\u88c5devtools\uff0c\u4e0b\u8f7d\u5728GitHub\u4e0a\u7684SCP\u5305<\/span><\/code><code><span class=\"code-snippet_outer\"># \u4e5f\u53ef\u4ee5\u9009\u62e9\u5728R\u73af\u5883\u4e2d\u8fdb\u884c\u4e0b\u8f7d\uff0c\u5c0f\u679c\u6bd4\u8f83\u504f\u5411\u4e8econda\u5b89\u88c5\u54e6~ \u8fd9\u4e2a\u9009\u62e9\u5e76\u6ca1\u6709\u5bf9\u9519\u4e4b\u5206\uff0c\u54ea\u79cd\u65b9\u6cd5\u66f4\u9002\u5408\u4f60\uff0c\u4e0b\u8f7d\u901f\u5ea6\u66f4\u5feb\uff0c\u4f7f\u7528\u4fbf\u662f\u5566\uff01\u4ee5\u7ed3\u679c\u4e3a\u5bfc\u5411\uff01\uff01<\/span><\/code><code><span class=\"code-snippet_outer\">if (!require(\"devtools\", quietly = TRUE)) {<\/span><\/code><code><span class=\"code-snippet_outer\"> install.packages(\"devtools\")<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">options(timeout = 999) # \u5728\u4f7f\u7528devtools::install_github()\u51fd\u6570\u65f6\uff0c\u90fd\u8981\u8bbe\u7f6e\u597d\u8fd9\u4e2a\u53c2\u6570\u54e6\uff0c\u5b83\u53ef\u4ee5\u5e2e\u52a9\u6211\u4eec\u987a\u5229\u7684\u4eceGitHub\u4e0a\u4e0b\u8f7dR\u5305<\/span><\/code><code><span class=\"code-snippet_outer\">devtools::install_github(\"zhanghao-njmu\/SCP\")<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u5728\u5b89\u88c5SCP\u7684\u8fc7\u7a0b\u4e2d\u53ef\u80fd\u4f1a\u51fa\u73b0\u67d0\u4e00\u5305\u65e0\u6cd5\u5b89\u88c5\u7684\u60c5\u51b5\uff0c\u6211\u4eec\u53ef\u4ee5\u4f7f\u7528conda\u8fdb\u884c\u5b89\u88c5\uff0c\u5b89\u88c5\u65b9\u6cd5\u4e0e\u5b89\u88c5devtools\u4e00\u81f4\uff0c\u5728\u8fd9\u5c0f\u679c\u5c31\u4e0d\u8d58\u8ff0\u5566\u3002<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<h1 style=\"text-align: center;\"><em><strong><span style=\"font-family: Calibri;font-size: 16pt;\">Windows<span style=\"font-family:\u5b8b\u4f53;\">\u7ec8\u7aef<\/span><\/span><\/strong><\/em><\/h1>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\">env_dir <- \"D:\/SCP_env\/\"<\/span><\/code><code><span class=\"code-snippet_outer\">dir.create(env_dir,recursive = TRUE)<\/span><\/code><code><span class=\"code-snippet_outer\">setwd(env_dir)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u4f7f\u7528\u5c0f\u679c\u66fe\u7ecf\u6559\u8fc7\u5927\u5bb6\u7684renv\u5305\uff0c\u5728\u672c\u5730\u521b\u5efa\u4e00\u4e2a\u65b0\u7684\u73af\u5883<\/span><\/code><code><span class=\"code-snippet_outer\">if(!require(\"renv\")) install.packages(renv)<\/span><\/code><code><span class=\"code-snippet_outer\">renv::init(project = env_dir)<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 14px;font-family: \u5b8b\u4f53;\"><br \/><\/span><\/p>\n<p><span style=\"font-size: 14px;\"><span style=\"font-size: 14px;font-family: Cambria;\"><span style=\"font-family: \u5b8b\u4f53;\">\u5728\u6210\u529f\u521b\u5efa\u73af\u5883\u540e\uff0c\u6211\u4eec\u53ef\u4ee5\u770b\u5230\u8fd9\u6837\u7684\u8f93\u51fa<\/span> <\/span><span style=\"font-family: Cambria;font-size: 12pt;\"><o:p><\/o:p><\/span><\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: \u5b8b\u4f53;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.175\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_TsqCGM3DibsnOBumZRvUkVgA7780uw-2.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4ETD3aPH8PDqwphkIicXu9G1wK2ibTsqCGM3DibsnOBumZRvUkVgA7780uw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"720\" style=\"\" \/><\/p>\n<p><br \/><\/p>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5728\u8fdb\u5165\u73af\u5883\u4e2d\uff0c\u6211\u4eec\u4fbf\u53ef\u4ee5\u5f00\u59cb\u5b89\u88c5R\u5305\uff0c\u548cLinux\u7ec8\u7aef\u4e00\u6837\uff0c\u6211\u4eec\u9996\u5148\u9700\u8981\u4e0b\u8f7d\u7684\u662fdevtools\u548cBiocManager\u4e24\u4e2aR\u5305\uff0c\u5bf9\u540e\u7eed\u7684R\u5305\u8fdb\u884c\u5b89\u88c5\u64cd\u4f5c\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:Cambria;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:12.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\">if (!require(\"devtools\", quietly = TRUE)) {<\/span><\/code><code><span class=\"code-snippet_outer\"> install.packages(\"devtools\")<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">options(timeout = 999)<\/span><\/code><code><span class=\"code-snippet_outer\">devtools::install_github(\"zhanghao-njmu\/SCP\")<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5728\u5b89\u88c5\u7684\u8fc7\u7a0b\u4e2d\uff0c\u6211\u4eec\u53ef\u80fd\u4f1a\u9047\u5230\u8fd9\u79cd\u60c5\u51b5<\/span><span style=\"mso-spacerun:'yes';font-family:Cambria;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:12.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2791666666666667\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_gCkgAx3Y0p6TsddSzTUtChpWibFTmA-2.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4E8kicMiaqhPAJgwFgvvJSYsHrCxBgCkgAx3Y0p6TsddSzTUtChpWibFTmA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"720\" style=\"\" \/><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5c0f\u4f19\u4f34\u4eec\uff0c\u9047\u5230\u62a5\u9519\u6211\u4eec\u4e0d\u8981\u5bb3\u6015\u54e6\uff0c\u8fd9\u4e2a\u62a5\u9519\u53ea\u662f\u56e0\u4e3a\u6211\u4eec\u7684\u4f9d\u8d56\u6ca1\u6709\u5b89\u88c5\u6210\u529f\u5462\uff01\u6211\u4eec\u53ea\u9700\u8981\u628a\u62a5\u9519\u4e0a\u7684\u624b\u52a8\u7684\u5b89\u88c5\u597d\u5c31\u53ef\u4ee5\u7ee7\u7eed\u8fd0\u884c\u5566<\/span><span style=\"mso-spacerun:'yes';font-family:Cambria;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:12.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\">if (!require(\"BiocManager\", quietly = TRUE)) {<\/span><\/code><code><span class=\"code-snippet_outer\"> install.packages(\"BiocManager\")<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">BiocManager::install(\"R_names\")<\/span><\/code><code><span class=\"code-snippet_outer\"># \u5982\u679c\u78b0\u4e0a\u7f51\u7edc\u4e0d\u7a33\u5b9a\u4e5f\u53ef\u4ee5\u8fdbbioconductor\u5b98\u7f51(https:\/\/www.bioconductor.org\/)\u4e0b\u8f7d\u5b89\u88c5\u5305\u540e\u8fdb\u884c\u672c\u5730\u4e0b\u8f7d\u54e6<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(\".tar.gz_dir\", repos = NULL, type = \"source\")<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u5b89\u88c5\u597dR\u8bed\u8a00\u73af\u5883\u4e0b\u7684R\u5305\u540e\uff0c\u6211\u4eec\u4fbf\u53ef\u4ee5\u8f7d\u5165SCP\u5305\uff0c\u51c6\u5907\u914d\u7f6epython\u73af\u5883\u4e0b\u7684\u5305\u5566\uff0c\u8fd9\u662f\u56e0\u4e3a\u5355\u7ec6\u80de\u5206\u6790\u7684\u8fc7\u7a0b\u4e2d\uff0c\u5982\uff1a\u901f\u7387\u5206\u6790\u7b49\u662f\u9700\u8981python\u73af\u5883\u7684\uff0c\u56e0\u6b64\u6211\u4eec\u5728\u672c\u5730\u4e2d\u4e5f\u9700\u8981\u4e0b\u8f7dconda\uff0c\u914d\u7f6e\u57fa\u7840\u7684python\u73af\u5883\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">options(reticulate.conda_binary = \"D:\/conda\/envs\/SCP_env\")# \u5728\u8fd9\u4e00\u6b65\u6211\u4eec\u9700\u8981\u6307\u5b9a\u597dconda\u7684\u73af\u5883\uff0c\u5207\u8bb0\u5207\u8bb0\uff01\u8def\u5f84\u6700\u540e\u4e0d\u8981\u52a0\u201c\/\u201d\u54e6\uff0c\u4e0d\u7136\u4f1a\u51fa\u73b0\u62a5\u9519<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u5728Windows\u7684\u5b89\u88c5\u8fc7\u7a0b\u4e2d\uff0c\u6211\u4eec\u8fd8\u9700\u8981\u989d\u5916\u5728conda\u4e2d\u5b89\u88c5\u4e00\u4e2a\u5305<\/span><\/code><code><span class=\"code-snippet_outer\">conda install jaxlib-0.1.70-cp38-none-win_amd64.whl<\/span><\/code><code><span class=\"code-snippet_outer\"># \u53ef\u4ee5\u4ece\u8fd9\u4e2a\u7f51\u5740\u4e2d\u4e0b\u8f7d\u9002\u5408\u81ea\u5df1\u7535\u8111\u7684\u5b89\u88c5\u5305\u54e6 https:\/\/whls.blob.core.windows.net\/unstable\/index.html<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u5b89\u88c5python\u5305\u6211\u4eec\u4f7f\u7528\u4e00\u4e0b\u547d\u4ee4<\/span><\/code><code><span class=\"code-snippet_outer\">SCP::PrepareEnv()<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u4f46\u8fd9\u6b65\u662f\u6709\u53ef\u80fd\u62a5\u9519\u7684\uff0c\u90e8\u5206\u5305\u56e0\u4e3a\u7248\u672c\u4e0d\u5bf9\u5e94\u7684\u6216\u662f\u65e0\u6cd5\u6b63\u5e38\u52a0\u8f7d\u7684\uff0c\u6211\u4eec\u90fd\u53ef\u4ee5\u6839\u636e\u62a5\u9519\u5355\u72ec\u7684\u8fdb\u884c\u4e0b\u8f7d\uff0c\u4f7f\u7528\u4ee5\u4e0b\u7684\u547d\u4ee4\uff08\u8fd9\u8fb9\u5c0f\u679c\u4f7f\u7528\u4e86pip\u7684\u4e34\u65f6\u6e05\u534e\u6e90\u8fdb\u884c\u4e0b\u8f7d\u54e6\uff09<\/span><\/code><code><span class=\"code-snippet_outer\">pip install -i https:\/\/pypi.tuna.tsinghua.edu.cn\/simple packages_names<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u65e0\u8bba\u662fLinux\u8fd8\u662fWindows\uff0c\u5f53\u80fd\u6210\u529f\u8fd0\u884c\u4ee5\u4e0b\u4ee3\u7801\u7684\u65f6\u5019\u5c31\u8bc1\u660e\u6211\u4eec\u57fa\u672c\u5df2\u7ecf\u6210\u529f\u5566\uff01\u4e3a\u4ec0\u4e48\u8bf4\u662f\u57fa\u672c\u5462\uff1f\u5c0f\u679c\u5728\u8fd9\u5148\u5356\u4e2a\u5173\u5b50\u54e6<o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"powershell\"><code><span class=\"code-snippet_outer\">library(SCP)<\/span><\/code><code><span class=\"code-snippet_outer\">data(\"pancreas_sub\")<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"kotlin\"><code><span class=\"code-snippet_outer\">pancreas_sub <- RunPAGA(srt = pancreas_sub, group_by = \"SubCellType\", linear_reduction = \"PCA\", nonlinear_reduction = \"UMAP\")<\/span><\/code><code><span class=\"code-snippet_outer\">## 'misc' slot is not converted.<\/span><\/code><code><span class=\"code-snippet_outer\">## 'tools' slot is not converted.<\/span><\/code><code><span class=\"code-snippet_outer\">CellDimPlot(pancreas_sub, group.by = \"SubCellType\", reduction = \"draw_graph_fr\")<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_cBCwnAr64SybN8LL1yNdmEEYnfpzlw-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4EwJIxs2NAiacAq58mWrnkkMOR4cBCwnAr64SybN8LL1yNdmEEYnfpzlw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\" \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code><span class=\"code-snippet_outer\">##\u7ec6\u80de\u805a\u7c7b<\/span><\/code><code><span class=\"code-snippet_outer\">CellDimPlot(<\/span><\/code><code><span class=\"code-snippet_outer\"> srt = pancreas_sub, group.by = c(\"CellType\", \"SubCellType\"),<\/span><\/code><code><span class=\"code-snippet_outer\"> reduction = \"UMAP\", theme_use = \"theme_blank\"<\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_9VeSNJlnwSddDhcaFFyjCYiajPH0sA-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4EPZA0LlQnNOk49vBVYVC9bb2gt9VeSNJlnwSddDhcaFFyjCYiajPH0sA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code><span class=\"code-snippet_outer\">##\u7ec6\u80de\u5468\u671f\u6ce8\u91ca<\/span><\/code><code><span class=\"code-snippet_outer\">CellDimPlot(<\/span><\/code><code><span class=\"code-snippet_outer\"> srt = pancreas_sub, group.by = \"SubCellType\", stat.by = \"Phase\",<\/span><\/code><code><span class=\"code-snippet_outer\"> reduction = \"UMAP\", theme_use = \"theme_blank\"<\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"display: none;line-height: 0px;\">\u200d<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_26pO8ZDnYTYXic5NzpbN0Gic134SUg-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4EJk5ibhLPdvLMak1kCg2pYJwELud26pO8ZDnYTYXic5NzpbN0Gic134SUg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\" \/><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code><span class=\"code-snippet_outer\">##\u7279\u5f81\u6ce8\u91ca<\/span><\/code><code><span class=\"code-snippet_outer\">FeatureDimPlot(<\/span><\/code><code><span class=\"code-snippet_outer\"> srt = pancreas_sub, features = c(\"Sox9\", \"Neurog3\", \"Fev\", \"Rbp4\"),<\/span><\/code><code><span class=\"code-snippet_outer\"> reduction = \"UMAP\", theme_use = \"theme_blank\"<\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_YWp1q7RgDsjMmTbaksPrEgNibiaWNw-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4ELMT78gsALSrzZIRQcKghXokDAeYWp1q7RgDsjMmTbaksPrEgNibiaWNw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\" \/><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">FeatureDimPlot(<\/span><\/code><code><span class=\"code-snippet_outer\">srt = pancreas_sub, features = c(\"Ins1\", \"Gcg\", \"Sst\", \"Ghrl\"),<\/span><\/code><code><span class=\"code-snippet_outer\">compare_features = TRUE, label = TRUE, label_insitu = TRUE,<\/span><\/code><code><span class=\"code-snippet_outer\">reduction = \"UMAP\", theme_use = \"theme_blank\"<\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_7ySWpJDQiakyT0YWEKLTeJk6UbQ52Q-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4EDGFiajR6gHX9J3OQXeUXOrCJMZ7ySWpJDQiakyT0YWEKLTeJk6UbQ52Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\"><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\">##\u7ec6\u80de\u7c7b\u578b\u3001\u767e\u5206\u6bd4\u548c\u7ec6\u80de\u5468\u671f<\/span><\/code><code><span class=\"code-snippet_outer\">ht <- GroupHeatmap(<\/span><\/code><code><span class=\"code-snippet_outer\"> srt = pancreas_sub,<\/span><\/code><code><span class=\"code-snippet_outer\"> features = c(<\/span><\/code><code><span class=\"code-snippet_outer\"> \"Sox9\", \"Anxa2\", # Ductal<\/span><\/code><code><span class=\"code-snippet_outer\"> \"Neurog3\", \"Hes6\", # EPs<\/span><\/code><code><span class=\"code-snippet_outer\"> \"Fev\", \"Neurod1\", # Pre-endocrine<\/span><\/code><code><span class=\"code-snippet_outer\"> \"Rbp4\", \"Pyy\", # Endocrine<\/span><\/code><code><span class=\"code-snippet_outer\"> \"Ins1\", \"Gcg\", \"Sst\", \"Ghrl\" # Beta, Alpha, Delta, Epsilon<\/span><\/code><code><span class=\"code-snippet_outer\"> ),<\/span><\/code><code><span class=\"code-snippet_outer\"> group.by = c(\"CellType\", \"SubCellType\"),<\/span><\/code><code><span class=\"code-snippet_outer\"> heatmap_palette = \"YlOrRd\",<\/span><\/code><code><span class=\"code-snippet_outer\"> cell_annotation = c(\"Phase\", \"G2M_score\", \"Cdh2\"),<\/span><\/code><code><span class=\"code-snippet_outer\"> cell_annotation_palette = c(\"Dark2\", \"Paired\", \"Paired\"),<\/span><\/code><code><span class=\"code-snippet_outer\"> show_row_names = TRUE, row_names_side = \"left\",<\/span><\/code><code><span class=\"code-snippet_outer\"> add_dot = TRUE, add_reticle = TRUE<\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><code><span class=\"code-snippet_outer\">print(ht$plot)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_nq3Fvzia9jiaeRtZmicPc4iaXTxAdg-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4ElHMjug0oqdzvbFq7DxSLT6qHyicJ6nq3Fvzia9jiaeRtZmicPc4iaXTxAdg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\"><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">##\u8d28\u63a7<\/span><\/code><code><span class=\"code-snippet_outer\">## conda install -c bioconda bioconductor-scdblfinder \u4f9d\u8d56\u4e0d\u9002\u914d<\/span><\/code><code><span class=\"code-snippet_outer\"># \u5b89\u88c5\u8def\u5f84<\/span><\/code><code><span class=\"code-snippet_outer\">pancreas_sub <- RunCellQC(srt = pancreas_sub)<\/span><\/code><code><span class=\"code-snippet_outer\">CellDimPlot(srt = pancreas_sub, group.by = \"CellQC\", reduction = \"UMAP\")<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><span style=\"display: none;line-height: 0px;\">\u200d<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_JeVpRcpPiaKQf2Jfx0uKI7bCAPvfIQ-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4Eibw9l344EgqkxqfBxJUrNiaia4d9JeVpRcpPiaKQf2Jfx0uKI7bCAPvfIQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\"><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code><span class=\"code-snippet_outer\">CellStatPlot(srt = pancreas_sub, stat.<span class=\"code-snippet__keyword\">by<\/span> = <span class=\"code-snippet__string\">\"CellQC\"<\/span>, <span class=\"code-snippet__keyword\">group<\/span>.<span class=\"code-snippet__keyword\">by<\/span> = <span class=\"code-snippet__string\">\"CellType\"<\/span>, label = TRUE)<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_SMT7rwMWS4KZiaD9YGSbNgribQW9RA-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4Euv3dNFLGrlPdoE2qCiaMFDqpeAySMT7rwMWS4KZiaD9YGSbNgribQW9RA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\"><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code><span class=\"code-snippet_outer\">CellStatPlot(<\/span><\/code><code><span class=\"code-snippet_outer\"> srt = pancreas_sub,<\/span><\/code><code><span class=\"code-snippet_outer\"> stat.by = c(<\/span><\/code><code><span class=\"code-snippet_outer\"> \"db_qc\", \"outlier_qc\", \"umi_qc\", \"gene_qc\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"mito_qc\", \"ribo_qc\", \"ribo_mito_ratio_qc\", \"species_qc\"<\/span><\/code><code><span class=\"code-snippet_outer\"> ),<\/span><\/code><code><span class=\"code-snippet_outer\"> plot_type = \"upset\", stat_level = \"Fail\"<\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_1REuHP9mfibFtRialZvebibPsMeIYw-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4Ek9Ifvu0PiaTD0FRZ1QQO7sqMnVWN1REuHP9mfibFtRialZvebibPsMeIYw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\"><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code><span class=\"code-snippet_outer\">##\u6807\u51c6\u6d41\u7a0b<\/span><\/code><code><span class=\"code-snippet_outer\">pancreas_sub <- Standard_SCP(srt = pancreas_sub)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">CellDimPlot(<\/span><\/code><code><span class=\"code-snippet_outer\"> srt = pancreas_sub, group.by = c(\"CellType\", \"SubCellType\"),<\/span><\/code><code><span class=\"code-snippet_outer\"> reduction = \"StandardUMAP2D\", theme_use = \"theme_blank\"<\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/12_QIkFYf1Z3SRIibibXLXxV3IxcZh95g-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwBJ4BvJe1iaoVp0piawdPJ4E6WprcWld7W73qoS6egpiaaKKfcKQIkFYf1Z3SRIibibXLXxV3IxcZh95g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"378\" style=\"\"><\/p>\n<section style=\"font-size: 16px;\">\n<section style=\"margin: 10px;text-align: left;justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;padding-right: 10px;padding-left: 10px;border-style: dashed;border-width: 1px;border-color: rgb(62, 62, 62);align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;border-width: 0px 0px 0px 10px;border-style: solid;border-left-color: rgb(144, 215, 236);border-right-color: rgb(46, 172, 109);box-shadow: rgb(0, 0, 0) 0px 0px 0px;border-bottom-left-radius: 0px;\">\n<section style=\"margin-right: 0%;margin-left: 0%;padding-right: 3px;padding-left: 3px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;margin-bottom: -0.65em;margin-top: 0.95em;\">\n<section style=\"width: 100%;\">\n<section style=\"border-top: 1px solid rgb(0, 0, 0);width: 99.9%;float: left;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"width: 6px;height: 6px;margin-top: -3px;border-radius: 100%;float: right;background-color: rgb(46, 172, 109);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"display: inline-block;margin-top: -1em;vertical-align: top;background-color: rgb(255, 255, 255);padding: 3px 10px;color: rgb(0, 154, 68);\">\n<p><strong>\u5f80\u671f\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section style=\"white-space: normal;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491794&idx=1&sn=2b7a4de95169c79202e44813885a8250&chksm=cf7f7017f808f9015689839fe4057fd418bf7b20eb1dfb5a28cd92e56a41b5605e62bfbc16fb&scene=21#wechat_redirect\" textvalue=\"1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4<\/a><br \/><\/section>\n<section style=\"white-space: normal;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491705&idx=1&sn=9ff9b6da28b3a256a99c6697d589be66&chksm=cf7f70bcf808f9aafd1019a0bba3dd3a55e2867dbb9a09203f13c2683adf867094637c405ed1&scene=21#wechat_redirect\" textvalue=\"2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86<\/a><\/section>\n<section style=\"white-space: normal;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491909&idx=1&sn=902e34bcf53e605fba2b457ec6a33e36&chksm=cf7f7180f808f8961542f458a49da14e85d8831449fb65fbf2623d55affc564686a5422f56fd&scene=21#wechat_redirect\" textvalue=\"3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8<\/a><\/section>\n<section style=\"white-space: normal;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491905&idx=1&sn=fb34ebd6282962c7803badaee7a39096&chksm=cf7f7184f808f8929608c0050344ad86193d0fc6455c1c18e16cc17bf4b37832b63d3b18284e&scene=21#wechat_redirect\" textvalue=\"4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b<\/a><\/section>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.4255555555555556\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/13_cTaYFqnWUYyNUD7oPfa3t3Zia6v9QQ-3.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxweqRKIWbRUBSWSGID9aogBsHn4PHBgzOlL5xiatzsIsLicPEcTaYFqnWUYyNUD7oPfa3t3Zia6v9QQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"900\" style=\"\"><\/p>\n<p><span style=\"font-size: 16px;\"><strong><span style=\"font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5982\u679c\u5c0f\u4f19\u4f34\u6709\u5176\u4ed6\u6570\u636e\u5206\u6790\u9700\u6c42\uff0c\u53ef\u4ee5\u626b\u7801\u4e86\u89e3Rstudio\u8d26\u53f7\uff0c\u6216\u8005\u70b9\u51fb\u539f\u6587\u8fdb\u884c\u4e86\u89e3~<\/span><\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-top: 0.5em;\">\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);\">\n<section style=\"text-align: left;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;\">\n<section style=\"margin-bottom: 8px;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u70b9\u51fb<strong><span style=\"color: rgb(0, 150, 136);\">\u201c\u9605\u8bfb\u539f\u6587\u201d<\/span><\/strong>\u7acb\u523b\u62e5\u6709<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 136);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u2193\u2193\u2193 <\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/wordpress\/?product=rstudio%E8%B4%A6%E5%8F%B7%EF%BC%8812%E4%B8%AA%E6%9C%88%EF%BC%89\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/wordpress\/?product=rstudio%E8%B4%A6%E5%8F%B7%EF%BC%8812%E4%B8%AA%E6%9C%88%EF%BC%89\" 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[…]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/52534"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=52534"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/52534\/revisions"}],"predecessor-version":[{"id":52548,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/52534\/revisions\/52548"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=52534"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=52534"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=52534"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}