<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 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right;letter-spacing: 4px;\" powered-by=\"xiumi.us\">\n<p><strong>\u70b9\u51fb\u84dd\u5b57<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;border-left: 4px solid rgb(242, 183, 183);border-bottom-left-radius: 0px;flex: 100 100 0%;align-self: stretch;height: auto;padding: 3px 10px;background-color: rgba(254, 245, 240, 0.8);\">\n<section style=\"color: rgb(181, 126, 121);font-size: 17px;letter-spacing: 4px;\" powered-by=\"xiumi.us\">\n<p><strong>\u5173\u6ce8\u5c0f\u56fe<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">Hello\u5927\u5bb6\u597d\uff0c\u5c0f\u56fe\u53c8\u6765\u5566<\/p>\n<p style=\"white-space: normal;\">\u5c0f\u56fe\u4eca\u5929\u4f7f\u7528HiC-Pro\u7ed9\u5927\u5bb6\u5206\u6790\u4e00\u4e0bHi-C\u7684\u6570\u636e<\/p>\n<p style=\"white-space: normal;\">\u9996\u5148\u719f\u6089\u4e09\u7ef4\u57fa\u56e0\u7ec4\u7684\u5c0f\u4f19\u4f34\u4eec\u80af\u5b9a\u90fd\u77e5\u9053Hi-C\u6570\u636e\u7684\u5904\u7406\u8f6f\u4ef6\u91cc\u9762HiC-Pro\u548cjuicer\u662f\u6bd4\u8f83\u6d41\u884c\u7684\uff0c<strong>\u90a3\u4e48\u8fd9\u4e24\u4e2a\u8f6f\u4ef6\u7684\u533a\u522b\u548c\u4fa7\u91cd\u70b9\u662f\u4ec0\u4e48\u5462\uff1f<\/strong>\u4e14\u542c\u5c0f\u56fe\u7ec6\u7ec6\u9053\u6765<\/p>\n<\/section>\n<ol style=\"padding-left: 40px;list-style-position: outside;\" powered-by=\"xiumi.us\" class=\"list-paddingleft-1\">\n<li>\n<p>HiC-Pro\u662f\u4e00\u4e2a\u7075\u6d3b\u548c\u9ad8\u6548\u7684\u7ba1\u9053\uff0c\u53ef\u4ee5\u4ece\u539f\u59cb\u6d4b\u5e8f\u6570\u636e\u5230\u89c4\u8303\u5316\u7684\u8054\u7cfb\u77e9\u9635\u3002\u5b83\u652f\u6301\u591a\u79cd\u9650\u5236\u6027\u9176\u5207\u4f4d\u70b9\uff0c\u53ef\u4ee5\u5e76\u884c\u5904\u7406\u591a\u4e2a\u6837\u672c\uff0c\u53ef\u4ee5\u68c0\u6d4b\u548c\u8fc7\u6ee4PCR\u91cd\u590d\uff0c\u53ef\u4ee5\u751f\u6210\u591a\u79cd\u683c\u5f0f\u7684\u8f93\u51fa\u6587\u4ef6\u3002<\/p>\n<\/li>\n<li>\n<p>juicer\u7531\u4e24\u90e8\u5206\u7ec4\u6210\uff1a\u4ece\u539f\u59cb\u6570\u636e\u5230\u521b\u5efaHi-C\u6587\u4ef6\u7684pipeline\u548c\u540e\u7eed\u5206\u6790\u5de5\u5177\u3002\u5b83\u53ef\u4ee5\u5904\u7406terabase\u89c4\u6a21\u7684Hi-C\u6570\u636e\u96c6\uff0c\u81ea\u52a8\u6ce8\u91caLoops\u548cDomains\uff0c\u4e0e\u591a\u4e2a\u96c6\u7fa4\u64cd\u4f5c\u7cfb\u7edf\u548cAmazon Web Services\u517c\u5bb9\u3002\u5b83\u8fd8\u53ef\u4ee5\u5229\u7528GPU\u8fdb\u884c\u5cf0\u503c\u8c03\u7528\u3002<\/p>\n<\/li>\n<li>\n<p>\u4e24\u8005\u7684\u4e3b\u8981\u533a\u522b\u5728\u4e8ejuicer\u63d0\u4f9b\u4e86\u4e00\u4e2a\u4e00\u952e\u5f0f\u7684\u7cfb\u7edf\uff0c\u800cHiC-Pro\u9700\u8981\u7528\u6237\u81ea\u5df1\u914d\u7f6e\u53c2\u6570\u548c\u8fd0\u884c\u6b65\u9aa4\u3002juicer\u8fd8\u63d0\u4f9b\u4e86\u66f4\u591a\u7684\u540e\u7eed\u5206\u6790\u5de5\u5177\uff0c\u59823D-DNA\uff0c\u53ef\u4ee5\u7528\u6765\u8f85\u52a9\u57fa\u56e0\u7ec4\u7ec4\u88c5\u3002HiC-Pro\u652f\u6301\u591a\u79cd\u9650\u5236\u6027\u9176\u5207\u4f4d\u70b9\uff0c\u800cjuicer\u53ea\u652f\u6301HindIII\u548cMboI\u4e24\u79cd\u3002HiC-Pro\u53ef\u4ee5\u751f\u6210\u591a\u79cd\u683c\u5f0f\u7684\u8f93\u51fa\u6587\u4ef6\uff0c\u5982bed, matrix, pairs\u7b49\uff0c\u800cjuicer\u53ea\u751f\u6210hic\u6587\u4ef6\u3002HiC-Pro\u53ef\u4ee5\u8ba9\u7528\u6237\u81ea\u5df1\u914d\u7f6e\u53c2\u6570\u548c\u8fd0\u884c\u6b65\u9aa4\uff0c\u6709\u66f4\u591a\u7684\u7075\u6d3b\u6027\u548c\u53ef\u5b9a\u5236\u6027\uff0c\u800cjuicer\u662f\u4e00\u4e2a\u4e00\u952e\u5f0f\u7684\u7cfb\u7edf\uff0c\u6709\u4e00\u4e9b\u9ed8\u8ba4\u7684\u8bbe\u7f6e\u548c\u9650\u5236\u3002<\/p>\n<\/li>\n<\/ol>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\">\u53ef\u80fd\u6709\u5c0f\u4f19\u4f34\u5bf9\u7b2c\u4e8c\u70b9\u4e2d\u7684terabase\u8868\u793a\u7591\u95ee\uff0c\u4e0d\u77e5\u9053\u662f\u4ec0\u4e48\uff0c\u5176\u5b9eterabase\u662f\u4e00\u4e2a\u8ba1\u91cf\u5355\u4f4d\uff0c\u8868\u793a10\u768412\u6b21\u65b9\u4e2a\u5b57\u8282\uff0c\u4e5f\u5c31\u662f\u4e00\u4e07\u4ebf\u4e2a\u5b57\u8282\u3002\u5b83\u901a\u5e38\u7528\u6765\u8868\u793a\u5927\u89c4\u6a21\u7684\u6570\u636e\u5b58\u50a8\u6216\u4f20\u8f93\u3002\u4f8b\u5982\uff0c\u4e00\u4e2aterabase\u7684Hi-C\u6570\u636e\u96c6\u5c31\u662f\u5305\u542b\u4e86\u4e00\u4e07\u4ebf\u4e2aHi-C\u6d4b\u5e8f\u5bf9\u7684\u6570\u636e\u96c6<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong>\u542c\u8bf4HiC-Pro\u8f6f\u4ef6\u5b89\u88c5\u5751\u5f88\u591a\uff1f\u5c0f\u56fe\u624b\u628a\u624b\u6559\u5927\u5bb6\u5b89\u88c5HiC-Pro\uff01<\/strong><\/p>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">1. \u9996\u5148\u521b\u5efa\u81ea\u5df1\u7684\u8f6f\u4ef6\u76ee\u5f55\u7136\u540e\u8fdb\u5165\uff0c\u63a5\u7740\u4e0b\u8f7dHiC-Pro\uff1a<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">wget https:<span class=\"code-snippet__comment\">\/\/github.com\/nservant\/HiC-Pro\/archive\/refs\/tags\/v3.1.0.tar.gz<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">2. \u89e3\u538b\u51fa\u6765\uff1a<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">tar -zxvf HiC-Pro<span class=\"code-snippet__number\">-3.1<\/span><span class=\"code-snippet__number\">.0<\/span>.tar.gz<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">3.\u4f7f\u7528conda\u6839\u636eyml\u6587\u4ef6\u521b\u5efa\u65b0\u7684\u73af\u5883<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">conda env create -f YOUR_PATH\/HiC-Pro<span class=\"code-snippet__number\">-3.1<\/span><span class=\"code-snippet__number\">.0<\/span>\/environment.yml -n hicpro<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">4. \u5207\u6362\u5230hicpro\u73af\u5883\u5e76\u5b89\u88c5HiC-Pro\uff08\u9700\u8981root\u6743\u9650\uff09<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">conda activate HiC-Pro<\/span><\/code><code><span class=\"code-snippet_outer\">make configure<\/span><\/code><code><span class=\"code-snippet_outer\">make<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u8fd9\u6837\u662f\u6700\u9002\u5408\u5c0f\u767d\u540c\u5b66\u7684\u5b89\u88c5\u5566\uff0c\u63a8\u8350\u4f7f\u7528\u5b98\u65b9\u7684yml\u6587\u4ef6\u521b\u5efa\u73af\u5883\uff0c\u5982\u679c\u662f\u81ea\u5df1\u521b\u5efa\u73af\u5883\u4e0b\u8f7d\u4f9d\u8d56\u7684\u8bdd\uff0c\u8bb0\u5f97\u53bb\u4fee\u6539config-install.txt\u6587\u4ef6\u7684\u5730\u5740\u54e6<br \/><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\">\u4e0b\u9762\u5c0f\u56fe\u5f00\u59cb\u5e26\u5927\u5bb6\u4e00\u8d77\u4f7f\u7528HiC-Pro\u6765\u5206\u6790HiC\u6570\u636e\u5566<\/p>\n<p style=\"white-space: normal;\">\u9996\u5148\u6211\u4eec\u9700\u8981\u7684\u6587\u4ef6\u662f\u53c2\u8003\u57fa\u56e0\u7ec4\uff0cbowtie2\u8f6f\u4ef6\u5efa\u7acb\u7684\u7d22\u5f15\uff0c\u542b\u6709\u9176\u5207\u7247\u6bb5\u4fe1\u606f\u7684bed\u6587\u4ef6\uff0c\u57fa\u56e0\u7ec4\u5927\u5c0f\u4fe1\u606f\u7684chrom.size\u6587\u4ef6\u4ee5\u53caHi-C\u7684\u6d4b\u5e8f\u6570\u636e\u96c6\uff08\u8fd9\u91cc\u5c0f\u56fe\u4f7f\u7528\u6d4b\u8bd5\u6570\u636e\u96c6\uff09<\/p>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"margin: 10px 0%;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;border-left: 5px solid rgb(242, 203, 203);border-bottom-left-radius: 0px;padding-left: 9px;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"margin: 2px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(110, 75, 36);font-size: 17px;line-height: 1.3;text-align: justify;letter-spacing: 1px;\">\n<p style=\"text-align: left;white-space: normal;\"><strong>\u9996\u5148\u4e0b\u8f7d\u6d4b\u8bd5\u6570\u636e\u96c6\uff1a<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">wget http:<span class=\"code-snippet__comment\">\/\/juicerawsmirror.s3.amazonaws.com\/opt\/juicer\/work\/HIC003\/fastq\/HIC003_S2_L001_R1_001.fastq.gz<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">wget http:<span class=\"code-snippet__comment\">\/\/juicerawsmirror.s3.amazonaws.com\/opt\/juicer\/work\/HIC003\/fastq\/HIC003_S2_L001_R2_001.fastq.gz<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u5c0f\u56fe\u53c2\u8003\u57fa\u56e0\u7ec4\u9009\u62e9\u7684\u662fhg38\uff0c\u63a5\u7740\u4f7f\u7528bowtie2-build\u6765\u5efa\u7acb\u7d22\u5f15\uff1a<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">bowtie2-build -f hg38.fasta hg38<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u53c2\u6570-f\u6307\u5b9a\u53c2\u8003\u57fa\u56e0\u7ec4\uff0c\u540e\u9762\u662f\u7d22\u5f15\u6587\u4ef6\u7684\u524d\u7f00\uff0c\u6700\u540e\u4f1a\u751f\u62106\u4e2abt2\u6587\u4ef6<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\">\u9176\u5207\u7247\u6bb5\u4fe1\u606f\u7684bed\u6587\u4ef6\u4f7f\u7528digest_genome.py\u6765\u751f\u6210\uff0cdigest_genome.py\u811a\u672c\u5728HiC-Pro-3.1.0\/bin\/utils\u76ee\u5f55\u4e0b\u9762\uff1a<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">python3 \/HiC-Pro<span class=\"code-snippet__number\">-3.1<\/span><span class=\"code-snippet__number\">.0<\/span>\/bin\/utils\/digest_genome.py hg38.fasta -r MboI -o hg38_MboI.bed<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u53c2\u6570-r\u6307\u5b9a\u9176\u5207\u79cd\u7c7b\uff0c\u4e0d\u533a\u5206\u5927\u5c0f\u5199<\/p>\n<p style=\"white-space: normal;\">\u53c2\u6570-o\u6307\u5b9a\u751f\u6210\u6587\u4ef6\u540d\u79f0<\/p>\n<p style=\"white-space: normal;\">\u5c0f\u679c\u751f\u6210\u7684hg38_MboI.bed\u6587\u4ef6\u5982\u4e0b\uff1a<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"0.8095238095238095\" data-s=\"300,640\" data-type=\"png\" data-w=\"483\" style=\"vertical-align: middle;width: 100%;height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_Pkj51bjKB8Z8CKggvSsaUYicKoZV8Q-9.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/2wJ7bWfS0QsB6fKmYL73GeH9qhSicicdRI6fyIX02Q9AbZicg5j8uYHxQTnAPkj51bjKB8Z8CKggvSsaUYicKoZV8Q\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u5176\u4e2d\u7b2c\u4e09\u5217\u662f\u68c0\u6d4b\u5230\u7684\u9176\u5207\u4f4d\u70b9\u5bf9\u5e94\u7684\u4f4d\u7f6e<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\">\u5c0f\u56fe\u4f7f\u7528samtools\u548cawk\u5de5\u5177\u6765\u5f97\u5230chrom.size\u6587\u4ef6\uff1a<\/p>\n<p style=\"white-space: normal;\">\u9996\u5148\u4f7f\u7528samtools\u8f6f\u4ef6\u5bf9\u53c2\u8003\u57fa\u56e0\u7ec4\u5efa\u7acb\u7d22\u5f15\uff1a<\/p>\n<p style=\"white-space: normal;\">samtools faidx hg38.fasta<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">samtools faidx hg38.fasta<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"margin: 10px 0%;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;border-left: 5px solid rgb(242, 203, 203);border-bottom-left-radius: 0px;padding-left: 9px;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"margin: 2px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(110, 75, 36);font-size: 17px;line-height: 1.3;text-align: justify;letter-spacing: 1px;\">\n<p style=\"white-space: normal;\"><strong>\u7136\u540e\u4f7f\u7528awk\u6765\u5bf9fai\u6587\u4ef6\u8fdb\u884c\u64cd\u4f5c\uff1a<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">awk <span class=\"code-snippet__string\">'{print $1 \"t\" $2}'<\/span> hg38.fasta.fai > hg38.chrom.sizes<\/span><\/code><\/pre>\n<\/section>\n<section style=\"color: rgb(182, 182, 182);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u4e0a\u9762\u6587\u4ef6\u7684\u51c6\u5907\u5de5\u4f5c\u5df2\u7ecf\u5b8c\u6210\u4e86\uff0c\u4e0b\u9762\u5f00\u59cb\u4f7f\u7528HiC-Pro\u8f6f\u4ef6\u8fdb\u884c\u5206\u6790\u4e86<br \/><\/p>\n<p style=\"white-space: normal;\">\u4e0d\u8fc7\u6211\u4eec\u9996\u5148\u8981\u5728HiC-Pro\u7684\u8f6f\u4ef6\u6839\u76ee\u5f55\u4e0b\u521b\u5efa\u4e00\u4e2arawdata\u6587\u4ef6\u5939\u6765\u5b58\u653e\u6211\u4eec\u7684\u6d4b\u5e8f\u6570\u636e\uff0c<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong>\u4e0b\u9762\u6211\u4eec\u8981\u4fee\u6539\u914d\u7f6e\u6587\u4ef6\uff1aconfig-hicpro.txt\uff0c\u4e0b\u9762\u662f\u4e00\u4e9b\u91cd\u8981\u7684\u8bbe\u7f6e\u53c2\u6570\uff1a<\/strong><\/p>\n<\/section>\n<ol style=\"padding-left: 40px;list-style-position: outside;\" powered-by=\"xiumi.us\" class=\"list-paddingleft-1\">\n<li>\n<p>N_CPU\uff0cCPU\u6570\u76ee\uff1b<\/p>\n<\/li>\n<li>\n<p>BOWTIE2_IDX_PATH\uff0c\u7d22\u5f15\u6240\u5728\u76ee\u5f55<\/p>\n<\/li>\n<li>\n<p>REFERENCE_GENOME\uff0c\u6bd4\u5bf9\u53c2\u8003\u57fa\u56e0\u7ec4\u8def\u5f84\u53ca\u524d\u7f00<\/p>\n<\/li>\n<li>\n<p>GENOME_SIZE\uff0cchrom.sizes\u6587\u4ef6\u7684\u8def\u5f84<\/p>\n<\/li>\n<li>\n<p>GENOME_FRAGMENT\uff0c\u9176\u5207\u7247\u6bb5\u7684bed\u6587\u4ef6\u7684\u8def\u5f84<\/p>\n<\/li>\n<li>\n<p>LIGATION_SITE\uff0c\u9176\u5207\u4f4d\u70b9\u672b\u7aef\u8865\u5e73\u518d\u6b21\u8fde\u63a5\u540e\u5f62\u6210\u7684\u5d4c\u5408\u5e8f\u5217\uff0c\u4f8b\u5982HindIII\uff0c\u5219\u4e3aAAGCTAGCTT\uff1b\u5982\u679c\u662fMboI\u5219\u5e8f\u5217\u4e3aGATCGATC\uff1b<\/p>\n<\/li>\n<\/ol>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"margin: 10px 0%;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;border-left: 5px solid rgb(242, 203, 203);border-bottom-left-radius: 0px;padding-left: 9px;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"margin: 2px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(110, 75, 36);font-size: 17px;line-height: 1.3;text-align: justify;letter-spacing: 1px;\">\n<p style=\"white-space: normal;\"><strong>\u914d\u7f6e\u597d\u6587\u4ef6\uff0c\u6211\u4eec\u5c31\u53ef\u4ee5\u8dd1\u8d77\u6765\u5566<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><strong>\u5c0f\u56fe\u7684\u4ee3\u7801\u5982\u4e0b\uff1a<\/strong><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">YOUR_PATH\/bin\/HiC-Pro -c YOUR_PATH\/config-hicpro.txt -i YOUR_PATH\/rawdata\/ -o YOUR_PATH\/test_re<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u5176\u4e2d\uff0c-c\u53c2\u6570\u6307\u5b9a\u914d\u7f6e\u6587\u4ef6\uff0c-i\u6307\u5b9a\u6570\u636e\u7684\u8def\u5f84\uff0c-o\u6307\u5b9a\u751f\u6210\u6587\u4ef6\u5939\u7684\u8def\u5f84<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"0.25245098039215685\" data-s=\"300,640\" data-type=\"png\" data-w=\"408\" style=\"vertical-align: middle;width: 100%;height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_xR54lsTZtsgAkszyicMmzWEJFCwtsQ-9.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/2wJ7bWfS0QsB6fKmYL73GeH9qhSicicdRIIh5OFEDiaEm9xmzGVAsm0cAWcKxR54lsTZtsgAkszyicMmzWEJFCwtsQ\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u751f\u6210\u7684\u65e5\u5fd7\u6587\u4ef6\u5728-o\u6307\u5b9a\u7684\u6587\u4ef6\u5939\u91cc\u9762<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong>\u4e0b\u9762\u662f\u5c0f\u56fe\u7684\u7ed3\u679c\u6587\u4ef6\uff1a<\/strong><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"1.17003367003367\" data-s=\"300,640\" data-type=\"png\" data-w=\"594\" style=\"vertical-align: middle;width: 100%;height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_c2c5CSqccoOqesjFcznvRKVPjSmUEA-9.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/2wJ7bWfS0QsB6fKmYL73GeH9qhSicicdRI1eQkG1tib1ibjzia95dIJg1Vl2vc2c5CSqccoOqesjFcznvRKVPjSmUEA\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u5176\u4e2d\uff0cbowtie_results:\u6bd4\u5bf9\u7ed3\u679c\u6240\u5728\u76ee\u5f55\uff1b<\/p>\n<p style=\"white-space: normal;\">hic_results:hic\u77e9\u9635\u53ca\u5206\u6790\u7ed3\u679c\u6240\u5728\u76ee\u5f55\uff1b<\/p>\n<p style=\"white-space: normal;\">logs:\u5b58\u653e\u5206\u6790\u65e5\u5fd7\uff1b<\/p>\n<p style=\"white-space: normal;\">rawdata\uff1a\u94fe\u63a5\u4e86\u539f\u59cb\u6570\u636e\uff1b<\/p>\n<p style=\"white-space: normal;\">tmp\uff1a\u5b58\u653e\u4e2d\u95f4\u6587\u4ef6<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong>\u76ee\u5f55bowtie_results\u4e0b\u5171\u6709\u4e09\u4e2a\u6587\u4ef6\u5939\uff1a<\/strong><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"0.5263157894736842\" data-s=\"300,640\" data-type=\"png\" data-w=\"285\" style=\"vertical-align: middle;width: 100%;height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_foia57kfX68aiaU1jf52bQ0MAnCvvg-9.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/2wJ7bWfS0QsB6fKmYL73GeH9qhSicicdRIyDjZK2234cEE76EibO0lg7KpRukfoia57kfX68aiaU1jf52bQ0MAnCvvg\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<ol style=\"padding-left: 40px;list-style-position: outside;\" powered-by=\"xiumi.us\" class=\"list-paddingleft-1\">\n<li>\n<p>bwt2\uff1a\u5b58\u653e\u5408\u5e76\u540e\u7684bam\u6587\u4ef6\u548c\u7edf\u8ba1\u7ed3\u679c<\/p>\n<\/li>\n<li>\n<p>bwt2_global\uff1a\u5b58\u653e\u5168\u5c40\u6bd4\u5bf9\u7ed3\u679c<\/p>\n<\/li>\n<li>\n<p>bwt2_local\uff1a\u5b58\u653e\u5c40\u90e8\u6bd4\u5bf9\u7ed3\u679c<\/p>\n<\/li>\n<\/ol>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong>\u76ee\u5f55hic_results\u4e0b\u9762\u6709\u56db\u4e2a\u6587\u4ef6\u5939\uff1a<\/strong><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"1.5287769784172662\" data-s=\"300,640\" data-type=\"png\" data-w=\"278\" style=\"vertical-align: middle;width: 100%;height: auto !important;\" 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href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzkxODUxNDgzNA==&mid=2247483741&idx=1&sn=39674bd0f28be4148fa07665bb9c0636&chksm=c1b17ca0f6c6f5b6cf63b01fdde27d73e61d5f8cfa2cddf644b400a32c6489de27a83b5b0b10&scene=21#wechat_redirect\" textvalue=\"Volcano plot\u2014\u2014\u522b\u518d\u95ee\u6211\u8fd9\u4e3a\u4ec0\u4e48\u662f\u706b\u5c71\u56fe\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">Volcano plot\u2014\u2014\u522b\u518d\u95ee\u6211\u8fd9\u4e3a\u4ec0\u4e48\u662f\u706b\u5c71\u56fe<\/a><\/p>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\ud83d\udc47\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u8fdb\u5165\u7f51\u5740<\/p>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u4e09\u5206\u949f\uff01HiC-Pro\u5e26\u4f60\u8dd1\u5b8cHi-C\u5206\u6790\u6d41\u7a0b \u5c0f\u56fe \u751f\u4fe1\u679c 20 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/53668"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=53668"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/53668\/revisions"}],"predecessor-version":[{"id":53675,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/53668\/revisions\/53675"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=53668"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=53668"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=53668"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

{"id":53668,"date":"2024-06-16T13:04:58","date_gmt":"2024-06-16T05:04:58","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=53668"},"modified":"2024-06-16T13:04:58","modified_gmt":"2024-06-16T05:04:58","slug":"%e4%b8%89%e5%88%86%e9%92%9f%ef%bc%81hic-pro%e5%b8%a6%e4%bd%a0%e8%b7%91%e5%ae%8chi-c%e5%88%86%e6%9e%90%e6%b5%81%e7%a8%8b-2","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=53668","title":{"rendered":"\u4e09\u5206\u949f\uff01HiC-Pro\u5e26\u4f60\u8dd1\u5b8cHi-C\u5206\u6790\u6d41\u7a0b"},"content":{"rendered":"

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#33ab01;margin-right:5px;}.ad{text-decoration:none;color:#d6d4d4;font-size:12px;}.msgBodyReply{padding-top:5px;}.userName span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation 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id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-07-16 19:00:05<\/em><\/div>\n<p><content><\/p>\n<div class=\"reprint\">\n<p>\u8f6c\u81ea\u516c\u4f17\u53f7\uff1a\u751f\u4fe1\u56fe<br \/><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzkxODUxNDgzNA==&mid=2247483794&idx=1&sn=f11f897a95432581180e0f9d3f378a0a\" target=\"_blank\" title=\"\u9605\u8bfb\u539f\u6587\" rel=\"noopener\">http:\/\/mp.weixin.qq.com\/s?__biz=MzkxODUxNDgzNA==&mid=2247483794&idx=1&sn=f11f897a95432581180e0f9d3f378a0a<\/a><\/p>\n<\/div>\n<section style=\"font-size: 16px;margin-bottom: 24px;\">\n<section style=\"display: flex;flex-flow: row nowrap;margin: 10px 0%;text-align: left;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: bottom;width: 50%;background-color: rgb(242, 183, 183);margin-right: 3px;flex: 0 0 auto;align-self: flex-end;height: auto;padding: 3px 10px;\">\n<section style=\"font-size: 17px;color: rgb(255, 255, 255);text-align: right;letter-spacing: 4px;\" powered-by=\"xiumi.us\">\n<p><strong>\u70b9\u51fb\u84dd\u5b57<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;border-left: 4px solid rgb(242, 183, 183);border-bottom-left-radius: 0px;flex: 100 100 0%;align-self: stretch;height: auto;padding: 3px 10px;background-color: rgba(254, 245, 240, 0.8);\">\n<section style=\"color: rgb(181, 126, 121);font-size: 17px;letter-spacing: 4px;\" powered-by=\"xiumi.us\">\n<p><strong>\u5173\u6ce8\u5c0f\u56fe<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">Hello\u5927\u5bb6\u597d\uff0c\u5c0f\u56fe\u53c8\u6765\u5566<\/p>\n<p style=\"white-space: normal;\">\u5c0f\u56fe\u4eca\u5929\u4f7f\u7528HiC-Pro\u7ed9\u5927\u5bb6\u5206\u6790\u4e00\u4e0bHi-C\u7684\u6570\u636e<\/p>\n<p style=\"white-space: normal;\">\u9996\u5148\u719f\u6089\u4e09\u7ef4\u57fa\u56e0\u7ec4\u7684\u5c0f\u4f19\u4f34\u4eec\u80af\u5b9a\u90fd\u77e5\u9053Hi-C\u6570\u636e\u7684\u5904\u7406\u8f6f\u4ef6\u91cc\u9762HiC-Pro\u548cjuicer\u662f\u6bd4\u8f83\u6d41\u884c\u7684\uff0c<strong>\u90a3\u4e48\u8fd9\u4e24\u4e2a\u8f6f\u4ef6\u7684\u533a\u522b\u548c\u4fa7\u91cd\u70b9\u662f\u4ec0\u4e48\u5462\uff1f<\/strong>\u4e14\u542c\u5c0f\u56fe\u7ec6\u7ec6\u9053\u6765<\/p>\n<\/section>\n<ol style=\"padding-left: 40px;list-style-position: outside;\" powered-by=\"xiumi.us\" class=\"list-paddingleft-1\">\n<li>\n<p>HiC-Pro\u662f\u4e00\u4e2a\u7075\u6d3b\u548c\u9ad8\u6548\u7684\u7ba1\u9053\uff0c\u53ef\u4ee5\u4ece\u539f\u59cb\u6d4b\u5e8f\u6570\u636e\u5230\u89c4\u8303\u5316\u7684\u8054\u7cfb\u77e9\u9635\u3002\u5b83\u652f\u6301\u591a\u79cd\u9650\u5236\u6027\u9176\u5207\u4f4d\u70b9\uff0c\u53ef\u4ee5\u5e76\u884c\u5904\u7406\u591a\u4e2a\u6837\u672c\uff0c\u53ef\u4ee5\u68c0\u6d4b\u548c\u8fc7\u6ee4PCR\u91cd\u590d\uff0c\u53ef\u4ee5\u751f\u6210\u591a\u79cd\u683c\u5f0f\u7684\u8f93\u51fa\u6587\u4ef6\u3002<\/p>\n<\/li>\n<li>\n<p>juicer\u7531\u4e24\u90e8\u5206\u7ec4\u6210\uff1a\u4ece\u539f\u59cb\u6570\u636e\u5230\u521b\u5efaHi-C\u6587\u4ef6\u7684pipeline\u548c\u540e\u7eed\u5206\u6790\u5de5\u5177\u3002\u5b83\u53ef\u4ee5\u5904\u7406terabase\u89c4\u6a21\u7684Hi-C\u6570\u636e\u96c6\uff0c\u81ea\u52a8\u6ce8\u91caLoops\u548cDomains\uff0c\u4e0e\u591a\u4e2a\u96c6\u7fa4\u64cd\u4f5c\u7cfb\u7edf\u548cAmazon Web Services\u517c\u5bb9\u3002\u5b83\u8fd8\u53ef\u4ee5\u5229\u7528GPU\u8fdb\u884c\u5cf0\u503c\u8c03\u7528\u3002<\/p>\n<\/li>\n<li>\n<p>\u4e24\u8005\u7684\u4e3b\u8981\u533a\u522b\u5728\u4e8ejuicer\u63d0\u4f9b\u4e86\u4e00\u4e2a\u4e00\u952e\u5f0f\u7684\u7cfb\u7edf\uff0c\u800cHiC-Pro\u9700\u8981\u7528\u6237\u81ea\u5df1\u914d\u7f6e\u53c2\u6570\u548c\u8fd0\u884c\u6b65\u9aa4\u3002juicer\u8fd8\u63d0\u4f9b\u4e86\u66f4\u591a\u7684\u540e\u7eed\u5206\u6790\u5de5\u5177\uff0c\u59823D-DNA\uff0c\u53ef\u4ee5\u7528\u6765\u8f85\u52a9\u57fa\u56e0\u7ec4\u7ec4\u88c5\u3002HiC-Pro\u652f\u6301\u591a\u79cd\u9650\u5236\u6027\u9176\u5207\u4f4d\u70b9\uff0c\u800cjuicer\u53ea\u652f\u6301HindIII\u548cMboI\u4e24\u79cd\u3002HiC-Pro\u53ef\u4ee5\u751f\u6210\u591a\u79cd\u683c\u5f0f\u7684\u8f93\u51fa\u6587\u4ef6\uff0c\u5982bed, matrix, pairs\u7b49\uff0c\u800cjuicer\u53ea\u751f\u6210hic\u6587\u4ef6\u3002HiC-Pro\u53ef\u4ee5\u8ba9\u7528\u6237\u81ea\u5df1\u914d\u7f6e\u53c2\u6570\u548c\u8fd0\u884c\u6b65\u9aa4\uff0c\u6709\u66f4\u591a\u7684\u7075\u6d3b\u6027\u548c\u53ef\u5b9a\u5236\u6027\uff0c\u800cjuicer\u662f\u4e00\u4e2a\u4e00\u952e\u5f0f\u7684\u7cfb\u7edf\uff0c\u6709\u4e00\u4e9b\u9ed8\u8ba4\u7684\u8bbe\u7f6e\u548c\u9650\u5236\u3002<\/p>\n<\/li>\n<\/ol>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\">\u53ef\u80fd\u6709\u5c0f\u4f19\u4f34\u5bf9\u7b2c\u4e8c\u70b9\u4e2d\u7684terabase\u8868\u793a\u7591\u95ee\uff0c\u4e0d\u77e5\u9053\u662f\u4ec0\u4e48\uff0c\u5176\u5b9eterabase\u662f\u4e00\u4e2a\u8ba1\u91cf\u5355\u4f4d\uff0c\u8868\u793a10\u768412\u6b21\u65b9\u4e2a\u5b57\u8282\uff0c\u4e5f\u5c31\u662f\u4e00\u4e07\u4ebf\u4e2a\u5b57\u8282\u3002\u5b83\u901a\u5e38\u7528\u6765\u8868\u793a\u5927\u89c4\u6a21\u7684\u6570\u636e\u5b58\u50a8\u6216\u4f20\u8f93\u3002\u4f8b\u5982\uff0c\u4e00\u4e2aterabase\u7684Hi-C\u6570\u636e\u96c6\u5c31\u662f\u5305\u542b\u4e86\u4e00\u4e07\u4ebf\u4e2aHi-C\u6d4b\u5e8f\u5bf9\u7684\u6570\u636e\u96c6<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong>\u542c\u8bf4HiC-Pro\u8f6f\u4ef6\u5b89\u88c5\u5751\u5f88\u591a\uff1f\u5c0f\u56fe\u624b\u628a\u624b\u6559\u5927\u5bb6\u5b89\u88c5HiC-Pro\uff01<\/strong><\/p>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">1. \u9996\u5148\u521b\u5efa\u81ea\u5df1\u7684\u8f6f\u4ef6\u76ee\u5f55\u7136\u540e\u8fdb\u5165\uff0c\u63a5\u7740\u4e0b\u8f7dHiC-Pro\uff1a<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">wget https:<span class=\"code-snippet__comment\">\/\/github.com\/nservant\/HiC-Pro\/archive\/refs\/tags\/v3.1.0.tar.gz<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">2. \u89e3\u538b\u51fa\u6765\uff1a<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">tar -zxvf HiC-Pro<span class=\"code-snippet__number\">-3.1<\/span><span class=\"code-snippet__number\">.0<\/span>.tar.gz<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">3.\u4f7f\u7528conda\u6839\u636eyml\u6587\u4ef6\u521b\u5efa\u65b0\u7684\u73af\u5883<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">conda env create -f YOUR_PATH\/HiC-Pro<span class=\"code-snippet__number\">-3.1<\/span><span class=\"code-snippet__number\">.0<\/span>\/environment.yml -n hicpro<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">4. \u5207\u6362\u5230hicpro\u73af\u5883\u5e76\u5b89\u88c5HiC-Pro\uff08\u9700\u8981root\u6743\u9650\uff09<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">conda activate HiC-Pro<\/span><\/code><code><span class=\"code-snippet_outer\">make configure<\/span><\/code><code><span class=\"code-snippet_outer\">make<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u8fd9\u6837\u662f\u6700\u9002\u5408\u5c0f\u767d\u540c\u5b66\u7684\u5b89\u88c5\u5566\uff0c\u63a8\u8350\u4f7f\u7528\u5b98\u65b9\u7684yml\u6587\u4ef6\u521b\u5efa\u73af\u5883\uff0c\u5982\u679c\u662f\u81ea\u5df1\u521b\u5efa\u73af\u5883\u4e0b\u8f7d\u4f9d\u8d56\u7684\u8bdd\uff0c\u8bb0\u5f97\u53bb\u4fee\u6539config-install.txt\u6587\u4ef6\u7684\u5730\u5740\u54e6<br \/><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\">\u4e0b\u9762\u5c0f\u56fe\u5f00\u59cb\u5e26\u5927\u5bb6\u4e00\u8d77\u4f7f\u7528HiC-Pro\u6765\u5206\u6790HiC\u6570\u636e\u5566<\/p>\n<p style=\"white-space: normal;\">\u9996\u5148\u6211\u4eec\u9700\u8981\u7684\u6587\u4ef6\u662f\u53c2\u8003\u57fa\u56e0\u7ec4\uff0cbowtie2\u8f6f\u4ef6\u5efa\u7acb\u7684\u7d22\u5f15\uff0c\u542b\u6709\u9176\u5207\u7247\u6bb5\u4fe1\u606f\u7684bed\u6587\u4ef6\uff0c\u57fa\u56e0\u7ec4\u5927\u5c0f\u4fe1\u606f\u7684chrom.size\u6587\u4ef6\u4ee5\u53caHi-C\u7684\u6d4b\u5e8f\u6570\u636e\u96c6\uff08\u8fd9\u91cc\u5c0f\u56fe\u4f7f\u7528\u6d4b\u8bd5\u6570\u636e\u96c6\uff09<\/p>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"margin: 10px 0%;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;border-left: 5px solid rgb(242, 203, 203);border-bottom-left-radius: 0px;padding-left: 9px;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"margin: 2px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(110, 75, 36);font-size: 17px;line-height: 1.3;text-align: justify;letter-spacing: 1px;\">\n<p style=\"text-align: left;white-space: normal;\"><strong>\u9996\u5148\u4e0b\u8f7d\u6d4b\u8bd5\u6570\u636e\u96c6\uff1a<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">wget http:<span class=\"code-snippet__comment\">\/\/juicerawsmirror.s3.amazonaws.com\/opt\/juicer\/work\/HIC003\/fastq\/HIC003_S2_L001_R1_001.fastq.gz<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">wget http:<span class=\"code-snippet__comment\">\/\/juicerawsmirror.s3.amazonaws.com\/opt\/juicer\/work\/HIC003\/fastq\/HIC003_S2_L001_R2_001.fastq.gz<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u5c0f\u56fe\u53c2\u8003\u57fa\u56e0\u7ec4\u9009\u62e9\u7684\u662fhg38\uff0c\u63a5\u7740\u4f7f\u7528bowtie2-build\u6765\u5efa\u7acb\u7d22\u5f15\uff1a<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">bowtie2-build -f hg38.fasta hg38<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u53c2\u6570-f\u6307\u5b9a\u53c2\u8003\u57fa\u56e0\u7ec4\uff0c\u540e\u9762\u662f\u7d22\u5f15\u6587\u4ef6\u7684\u524d\u7f00\uff0c\u6700\u540e\u4f1a\u751f\u62106\u4e2abt2\u6587\u4ef6<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\">\u9176\u5207\u7247\u6bb5\u4fe1\u606f\u7684bed\u6587\u4ef6\u4f7f\u7528digest_genome.py\u6765\u751f\u6210\uff0cdigest_genome.py\u811a\u672c\u5728HiC-Pro-3.1.0\/bin\/utils\u76ee\u5f55\u4e0b\u9762\uff1a<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">python3 \/HiC-Pro<span class=\"code-snippet__number\">-3.1<\/span><span class=\"code-snippet__number\">.0<\/span>\/bin\/utils\/digest_genome.py hg38.fasta -r MboI -o hg38_MboI.bed<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u53c2\u6570-r\u6307\u5b9a\u9176\u5207\u79cd\u7c7b\uff0c\u4e0d\u533a\u5206\u5927\u5c0f\u5199<\/p>\n<p style=\"white-space: normal;\">\u53c2\u6570-o\u6307\u5b9a\u751f\u6210\u6587\u4ef6\u540d\u79f0<\/p>\n<p style=\"white-space: normal;\">\u5c0f\u679c\u751f\u6210\u7684hg38_MboI.bed\u6587\u4ef6\u5982\u4e0b\uff1a<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"0.8095238095238095\" data-s=\"300,640\" data-type=\"png\" data-w=\"483\" style=\"vertical-align: middle;width: 100%;height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_Pkj51bjKB8Z8CKggvSsaUYicKoZV8Q-9.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/2wJ7bWfS0QsB6fKmYL73GeH9qhSicicdRI6fyIX02Q9AbZicg5j8uYHxQTnAPkj51bjKB8Z8CKggvSsaUYicKoZV8Q\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u5176\u4e2d\u7b2c\u4e09\u5217\u662f\u68c0\u6d4b\u5230\u7684\u9176\u5207\u4f4d\u70b9\u5bf9\u5e94\u7684\u4f4d\u7f6e<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\">\u5c0f\u56fe\u4f7f\u7528samtools\u548cawk\u5de5\u5177\u6765\u5f97\u5230chrom.size\u6587\u4ef6\uff1a<\/p>\n<p style=\"white-space: normal;\">\u9996\u5148\u4f7f\u7528samtools\u8f6f\u4ef6\u5bf9\u53c2\u8003\u57fa\u56e0\u7ec4\u5efa\u7acb\u7d22\u5f15\uff1a<\/p>\n<p style=\"white-space: normal;\">samtools faidx hg38.fasta<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">samtools faidx hg38.fasta<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"margin: 10px 0%;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;border-left: 5px solid rgb(242, 203, 203);border-bottom-left-radius: 0px;padding-left: 9px;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"margin: 2px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(110, 75, 36);font-size: 17px;line-height: 1.3;text-align: justify;letter-spacing: 1px;\">\n<p style=\"white-space: normal;\"><strong>\u7136\u540e\u4f7f\u7528awk\u6765\u5bf9fai\u6587\u4ef6\u8fdb\u884c\u64cd\u4f5c\uff1a<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">awk <span class=\"code-snippet__string\">'{print $1 \"t\" $2}'<\/span> hg38.fasta.fai > hg38.chrom.sizes<\/span><\/code><\/pre>\n<\/section>\n<section style=\"color: rgb(182, 182, 182);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u4e0a\u9762\u6587\u4ef6\u7684\u51c6\u5907\u5de5\u4f5c\u5df2\u7ecf\u5b8c\u6210\u4e86\uff0c\u4e0b\u9762\u5f00\u59cb\u4f7f\u7528HiC-Pro\u8f6f\u4ef6\u8fdb\u884c\u5206\u6790\u4e86<br \/><\/p>\n<p style=\"white-space: normal;\">\u4e0d\u8fc7\u6211\u4eec\u9996\u5148\u8981\u5728HiC-Pro\u7684\u8f6f\u4ef6\u6839\u76ee\u5f55\u4e0b\u521b\u5efa\u4e00\u4e2arawdata\u6587\u4ef6\u5939\u6765\u5b58\u653e\u6211\u4eec\u7684\u6d4b\u5e8f\u6570\u636e\uff0c<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong>\u4e0b\u9762\u6211\u4eec\u8981\u4fee\u6539\u914d\u7f6e\u6587\u4ef6\uff1aconfig-hicpro.txt\uff0c\u4e0b\u9762\u662f\u4e00\u4e9b\u91cd\u8981\u7684\u8bbe\u7f6e\u53c2\u6570\uff1a<\/strong><\/p>\n<\/section>\n<ol style=\"padding-left: 40px;list-style-position: outside;\" powered-by=\"xiumi.us\" class=\"list-paddingleft-1\">\n<li>\n<p>N_CPU\uff0cCPU\u6570\u76ee\uff1b<\/p>\n<\/li>\n<li>\n<p>BOWTIE2_IDX_PATH\uff0c\u7d22\u5f15\u6240\u5728\u76ee\u5f55<\/p>\n<\/li>\n<li>\n<p>REFERENCE_GENOME\uff0c\u6bd4\u5bf9\u53c2\u8003\u57fa\u56e0\u7ec4\u8def\u5f84\u53ca\u524d\u7f00<\/p>\n<\/li>\n<li>\n<p>GENOME_SIZE\uff0cchrom.sizes\u6587\u4ef6\u7684\u8def\u5f84<\/p>\n<\/li>\n<li>\n<p>GENOME_FRAGMENT\uff0c\u9176\u5207\u7247\u6bb5\u7684bed\u6587\u4ef6\u7684\u8def\u5f84<\/p>\n<\/li>\n<li>\n<p>LIGATION_SITE\uff0c\u9176\u5207\u4f4d\u70b9\u672b\u7aef\u8865\u5e73\u518d\u6b21\u8fde\u63a5\u540e\u5f62\u6210\u7684\u5d4c\u5408\u5e8f\u5217\uff0c\u4f8b\u5982HindIII\uff0c\u5219\u4e3aAAGCTAGCTT\uff1b\u5982\u679c\u662fMboI\u5219\u5e8f\u5217\u4e3aGATCGATC\uff1b<\/p>\n<\/li>\n<\/ol>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"margin: 10px 0%;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;border-left: 5px solid rgb(242, 203, 203);border-bottom-left-radius: 0px;padding-left: 9px;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"margin: 2px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(110, 75, 36);font-size: 17px;line-height: 1.3;text-align: justify;letter-spacing: 1px;\">\n<p style=\"white-space: normal;\"><strong>\u914d\u7f6e\u597d\u6587\u4ef6\uff0c\u6211\u4eec\u5c31\u53ef\u4ee5\u8dd1\u8d77\u6765\u5566<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><strong>\u5c0f\u56fe\u7684\u4ee3\u7801\u5982\u4e0b\uff1a<\/strong><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">YOUR_PATH\/bin\/HiC-Pro -c YOUR_PATH\/config-hicpro.txt -i YOUR_PATH\/rawdata\/ -o YOUR_PATH\/test_re<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u5176\u4e2d\uff0c-c\u53c2\u6570\u6307\u5b9a\u914d\u7f6e\u6587\u4ef6\uff0c-i\u6307\u5b9a\u6570\u636e\u7684\u8def\u5f84\uff0c-o\u6307\u5b9a\u751f\u6210\u6587\u4ef6\u5939\u7684\u8def\u5f84<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"0.25245098039215685\" data-s=\"300,640\" data-type=\"png\" data-w=\"408\" style=\"vertical-align: middle;width: 100%;height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_xR54lsTZtsgAkszyicMmzWEJFCwtsQ-9.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/2wJ7bWfS0QsB6fKmYL73GeH9qhSicicdRIIh5OFEDiaEm9xmzGVAsm0cAWcKxR54lsTZtsgAkszyicMmzWEJFCwtsQ\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u751f\u6210\u7684\u65e5\u5fd7\u6587\u4ef6\u5728-o\u6307\u5b9a\u7684\u6587\u4ef6\u5939\u91cc\u9762<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong>\u4e0b\u9762\u662f\u5c0f\u56fe\u7684\u7ed3\u679c\u6587\u4ef6\uff1a<\/strong><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"1.17003367003367\" data-s=\"300,640\" data-type=\"png\" data-w=\"594\" style=\"vertical-align: middle;width: 100%;height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_c2c5CSqccoOqesjFcznvRKVPjSmUEA-9.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/2wJ7bWfS0QsB6fKmYL73GeH9qhSicicdRI1eQkG1tib1ibjzia95dIJg1Vl2vc2c5CSqccoOqesjFcznvRKVPjSmUEA\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u5176\u4e2d\uff0cbowtie_results:\u6bd4\u5bf9\u7ed3\u679c\u6240\u5728\u76ee\u5f55\uff1b<\/p>\n<p style=\"white-space: normal;\">hic_results:hic\u77e9\u9635\u53ca\u5206\u6790\u7ed3\u679c\u6240\u5728\u76ee\u5f55\uff1b<\/p>\n<p style=\"white-space: normal;\">logs:\u5b58\u653e\u5206\u6790\u65e5\u5fd7\uff1b<\/p>\n<p style=\"white-space: normal;\">rawdata\uff1a\u94fe\u63a5\u4e86\u539f\u59cb\u6570\u636e\uff1b<\/p>\n<p style=\"white-space: normal;\">tmp\uff1a\u5b58\u653e\u4e2d\u95f4\u6587\u4ef6<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong>\u76ee\u5f55bowtie_results\u4e0b\u5171\u6709\u4e09\u4e2a\u6587\u4ef6\u5939\uff1a<\/strong><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"0.5263157894736842\" data-s=\"300,640\" data-type=\"png\" data-w=\"285\" style=\"vertical-align: middle;width: 100%;height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_foia57kfX68aiaU1jf52bQ0MAnCvvg-9.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/2wJ7bWfS0QsB6fKmYL73GeH9qhSicicdRIyDjZK2234cEE76EibO0lg7KpRukfoia57kfX68aiaU1jf52bQ0MAnCvvg\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<ol style=\"padding-left: 40px;list-style-position: outside;\" powered-by=\"xiumi.us\" class=\"list-paddingleft-1\">\n<li>\n<p>bwt2\uff1a\u5b58\u653e\u5408\u5e76\u540e\u7684bam\u6587\u4ef6\u548c\u7edf\u8ba1\u7ed3\u679c<\/p>\n<\/li>\n<li>\n<p>bwt2_global\uff1a\u5b58\u653e\u5168\u5c40\u6bd4\u5bf9\u7ed3\u679c<\/p>\n<\/li>\n<li>\n<p>bwt2_local\uff1a\u5b58\u653e\u5c40\u90e8\u6bd4\u5bf9\u7ed3\u679c<\/p>\n<\/li>\n<\/ol>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong>\u76ee\u5f55hic_results\u4e0b\u9762\u6709\u56db\u4e2a\u6587\u4ef6\u5939\uff1a<\/strong><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"1.5287769784172662\" data-s=\"300,640\" data-type=\"png\" data-w=\"278\" style=\"vertical-align: middle;width: 100%;height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_2z69tzURz8aGjRhRkB0ZYhuricghNQ-9.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/2wJ7bWfS0QsB6fKmYL73GeH9qhSicicdRIoceBFka8ecArbSicemBlv8oNDr2z69tzURz8aGjRhRkB0ZYhuricghNQ\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<ol style=\"padding-left: 40px;list-style-position: outside;\" powered-by=\"xiumi.us\" class=\"list-paddingleft-1\">\n<li>\n<p>data\uff1a\u5b58\u653evalid pair reads\u53ca\u5176\u4ed6\u6570\u636e\u6587\u4ef6<\/p>\n<\/li>\n<li>\n<p>matrix\uff1a\u5b58\u653e\u4e0d\u540c\u5206\u8fa8\u7387\u77e9\u9635\u6587\u4ef6<\/p>\n<\/li>\n<li>\n<p>pic\uff1a\u5b58\u653e\u7edf\u8ba1\u5206\u6790\u56fe\u7247<\/p>\n<\/li>\n<li>\n<p>stats\uff1a\u5b58\u653e\u7edf\u8ba1\u8868<\/p>\n<\/li>\n<\/ol>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: 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normal;\">\u4eca\u5929\u7684\u5173\u4e8eHiC-Pro\u8f6f\u4ef6\u7684\u5b66\u4e60\u5c31\u5230\u8fd9\u91cc\u5566\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u627e\u5c0f\u56fe\u8ba8\u8bba\uff0c\u6709\u611f\u89c9\u751f\u4fe1\u5206\u6790\u590d\u6742\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u76f4\u63a5\u4f7f\u7528\u6211\u4eec\u7684\u751f\u4fe1\u5c0f\u5de5\u5177\u54e6\uff0c\u94fe\u63a5\u5728\u8fd9\uff1ahttp:\/\/www.biocloudservice.com\/home.html\uff0c\u6211\u4eec\u660e\u5929\u89c1\u54af~<\/p>\n<p style=\"white-space: normal;\">\u6b22\u8fce\u4f7f\u7528\uff1a\u4e91\u751f\u4fe1\u5e73\u53f0 ( http:\/\/www.biocloudservice.com\/home.html)<\/p>\n<\/section>\n<\/section>\n<section style=\"transform: scale(0.5);transform-origin: center center;margin-top: -65px;margin-bottom: -65px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img 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opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:49.classicTable1:2.td@@0\" style=\"border-top: 0px none rgb(62, 62, 62);border-right: 0px none rgb(62, 62, 62);border-left: 0px none rgb(62, 62, 62);border-bottom-color: rgb(202, 198, 198);border-bottom-style: dashed;background-color: rgb(247, 247, 247);padding-right: 5px;padding-left: 5px;\" width=\"96.3700%\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: middle;border-width: 0px;align-self: center;flex: 0 0 auto;\">\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzkxODUxNDgzNA==&mid=2247483730&idx=1&sn=abc7095012e15da0a0c27ec4d79ec243&chksm=c1b17caff6c6f5b93c5fc3b02da32d7e65aaf6063f50cde671373bf81595115c30ae0235ffb1&scene=21#wechat_redirect\" textvalue=\"GSEA\u5bcc\u96c6\u5206\u6790\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">GSEA\u5bcc\u96c6\u5206\u6790<\/a><\/p>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:49.classicTable1:3\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:49.classicTable1:3.td@@0\" style=\"border-top: 0px none rgb(62, 62, 62);border-right: 0px none rgb(62, 62, 62);border-left: 0px none rgb(62, 62, 62);border-bottom-color: rgb(202, 198, 198);border-bottom-style: dashed;background-color: rgb(247, 247, 247);padding-right: 5px;padding-left: 5px;\" width=\"96.3700%\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: middle;border-width: 0px;align-self: center;flex: 0 0 auto;\">\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzkxODUxNDgzNA==&mid=2247483741&idx=1&sn=39674bd0f28be4148fa07665bb9c0636&chksm=c1b17ca0f6c6f5b6cf63b01fdde27d73e61d5f8cfa2cddf644b400a32c6489de27a83b5b0b10&scene=21#wechat_redirect\" textvalue=\"Volcano plot\u2014\u2014\u522b\u518d\u95ee\u6211\u8fd9\u4e3a\u4ec0\u4e48\u662f\u706b\u5c71\u56fe\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">Volcano plot\u2014\u2014\u522b\u518d\u95ee\u6211\u8fd9\u4e3a\u4ec0\u4e48\u662f\u706b\u5c71\u56fe<\/a><\/p>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\ud83d\udc47\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u8fdb\u5165\u7f51\u5740<\/p>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u4e09\u5206\u949f\uff01HiC-Pro\u5e26\u4f60\u8dd1\u5b8cHi-C\u5206\u6790\u6d41\u7a0b \u5c0f\u56fe \u751f\u4fe1\u679c 20 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/53668"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=53668"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/53668\/revisions"}],"predecessor-version":[{"id":53675,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/53668\/revisions\/53675"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=53668"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=53668"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=53668"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}