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style=\"display: inline-block;vertical-align: middle;width: 6px;height: 6px;border-radius: 100%;background-color: rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;padding-right: 18px;padding-left: 18px;\">\n<section style=\"text-align: center;margin-top: 20px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 13px 8px 0px;border-color: rgb(93, 91, 92) rgba(255, 255, 255, 0) rgb(251, 133, 129);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;height: auto;align-self: center;\">\n<section style=\"overflow: hidden;text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: 6px;height: 6px;\">\n<section style=\"transform: rotate(0.1deg);\">\n<section style=\"width: 6px;height: 6px;border-radius: 100%;background-color: rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 100%;margin-right: -6px;margin-left: -7px;border-bottom: 2px dotted rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 6px;height: 6px;border-radius: 100%;background-color: rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.8421052631578947\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_WgT3XTQbJQWicmmCf0S1iarXjJpptA-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSHwEuTm51NEXRqZDxBUdecaX3KofibdS6cTuWxOgpp3Su6THrWgT3XTQbJQWicmmCf0S1iarXjJpptA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"361\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">\u53ef\u4ee5\u770b\u5230\u683c\u5f0f\u975e\u5e38\u7b80\u5355\uff0c\u6bcf\u4e00\u884c\u5bf9\u5e94\u4e00\u4e2a\u6837\u672c\uff0c\u7b2c\u4e00\u5217\u4e3a\u6837\u672c\u540d\uff0c\u7b2c\u4e8c\u5217\u4e3a\u6837\u672c\u5206\u7ec4\uff0c\u540e\u9762\u51e0\u5217\u5c31\u662f\u6211\u4eec\u9700\u8981\u6bd4\u8f83\u7684\u6307\u6807\u4e86\uff0c\u53ef\u4ee5\u662f\u4e00\u5217\u4e5f\u53ef\u4ee5\u662f\u591a\u5217\uff0c\u7136\u540e\u8ba9\u6211\u4eec\u5f00\u59cb\u4ee3\u7801\u90e8\u5206\u5427\uff01<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u52a0\u8f7d\u5173\u952eR\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u52a0\u8f7d\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">rt<-read.table(<span class=\"code-snippet__string\">\"gene_deg.txt\"<\/span>,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,header=<span class=\"code-snippet__built_in\">T<\/span>,check.names=<span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u9884\u89c8\u4e00\u4e0b<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">head(rt)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.34794520547945207\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_hyuRIQJCiaEWrfqmpoDyj1Y2m0iaWA-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSHwEuTm51NEXRqZDxBUdecjRHlIhIZDHbRzG78tn73nn7XkHhyuRIQJCiaEWrfqmpoDyj1Y2m0iaWA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"365\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7136\u540e\u6307\u5b9a\u6211\u4eec\u7684\u5206\u7ec4\u548c\u5bf9\u6bd4\u6307\u6807<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">x=colnames(rt)[<span class=\"code-snippet__number\">2<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">y1=colnames(rt)[<span class=\"code-snippet__number\">3<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">y2=colnames(rt)[<span class=\"code-snippet__number\">4<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">y3=colnames(rt)[<span class=\"code-snippet__number\">5<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5206\u7ec4\u9700\u8981\u8fdb\u884c\u4e00\u4e0b\u56e0\u5b50\u5316\uff0c\u56e0\u4e3a\u662f\u5206\u7c7b\u53d8\u91cf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">group=levels(factor(rt$group))<\/span><\/code><code><span class=\"code-snippet_outer\">rt$group=factor(rt$group,levels=group)<\/span><\/code><code><span class=\"code-snippet_outer\">comp=combn(group,<span class=\"code-snippet__number\">2<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">my_comparisons=<span class=\"code-snippet__built_in\">list<\/span>()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(i <span class=\"code-snippet__keyword\">in<\/span> <span class=\"code-snippet__number\">1<\/span>:ncol(comp)){my_comparisons[[i]]<-comp[,i]}<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\">#\u7136\u540e\u9700\u8981\u8c03\u7528ggpubr\u4e2d\u7684ggboxplot\u65b9\u6cd5\u6765\u8fdb\u884c\u53ef\u89c6\u5316<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> ggboxplot(rt,<\/span><\/code><code><span class=\"code-snippet_outer\">x=<span class=\"code-snippet__string\">\"group\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">y=y1,<\/span><\/code><code><span class=\"code-snippet_outer\">color=<span class=\"code-snippet__string\">\"group\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">xlab=<span class=\"code-snippet__string\">\"\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">ylab= <span class=\"code-snippet__string\">\" Expression\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">legend.title=<span class=\"code-snippet__string\">\"Type\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">palette = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"#dc0000\"<\/span>,<span class=\"code-snippet__string\">\"#0072b5\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">add = <span class=\"code-snippet__string\">\"jitter\"<\/span>)+ <\/span><\/code><code><span class=\"code-snippet_outer\">stat_compare_means(comparisons = my_comparisons,method = <span class=\"code-snippet__string\">\"wilcox.test\"<\/span>,symnum.args=<span class=\"code-snippet__built_in\">list<\/span>(cutpoints = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__number\">0<\/span>, <span class=\"code-snippet__number\">0.001<\/span>, <span class=\"code-snippet__number\">0.01<\/span>, <span class=\"code-snippet__number\">0.05<\/span>, <span class=\"code-snippet__number\">1<\/span>), symbols = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"***\"<\/span>, <span class=\"code-snippet__string\">\"**\"<\/span>, <span class=\"code-snippet__string\">\"*\"<\/span>, <span class=\"code-snippet__string\">\"-\"<\/span>)),label = <span class=\"code-snippet__string\">\"p.signif\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\">#\u8fd9\u91cc\u5927\u6d77\u54e5\u7528*\u53f7\u6765\u4ee3\u66ff\u4e86\u5dee\u5f02\u6027P\u503c\uff0c\u5176\u4e2d\u5c0f\u4e8e1\u5927\u4e8e0.05\u5c31\u662f\u4e00\u4e2a-\uff0c\u7136\u540e\u5c0f\u4e8e0.05\u5927\u4e8e0.01\u5c31\u662f\u4e00\u4e2a*\uff0c\u5c0f\u4e8e0.01\u5927\u4e8e0.001\u5c31\u662f**\uff0c\u5c0f\u4e8e0.001\u5927\u4e8e0\u5c31\u662f***<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5f53\u7136\u4e86\u5927\u5bb6\u4e5f\u53ef\u4ee5\u76f4\u63a5\u663e\u793ap\u503c\u54e6\uff0c\u53ea\u9700\u8981\u628asymnum.args=list(cutpoints = c(0, 0.001, 0.01, 0.05, 1), symbols = c(\"***\", \"**\", \"*\", \"-\")),label = \"p.signif\")\u53bb\u6389\u5c31\u597d\u5566\uff01\u4f1a\u81ea\u52a8\u4ee5\u79d1\u5b66\u8ba1\u6570\u6cd5\u7684\u65b9\u5f0f\u5c55\u793aP\u503c\u54e6\uff01<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\">#\u6211\u4eec\u5148\u770b\u770b*\u53f7\u663e\u793a\u7684\u7ed3\u679c<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9329896907216495\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_a2JuQ9RGyJ2nAej7KWpvMGT7uO4pMg-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSHwEuTm51NEXRqZDxBUdecYwNzoo0qYTJZOgUJ32IKrFiaUia2JuQ9RGyJ2nAej7KWpvMGT7uO4pMg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"388\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u770b\u7740\u662f\u4e0d\u662f\u5f88\u4e0d\u9519<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6211\u4eec\u518d\u770b\u770b\u76f4\u63a5\u663e\u793aP\u503c\u7684<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9692307692307692\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_abYVUkW50P9ic7CXfXvgPuYDZYnC9g-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSHwEuTm51NEXRqZDxBUdecyIXGIQCib0ddz7YqqZ3Oib1tAEJabYVUkW50P9ic7CXfXvgPuYDZYnC9g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"390\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u800c\u4e14stat_compare_means\u4e2d\u7684method\u53ef\u4ee5\u6307\u5b9a\u4e0d\u540c\u7684\u5dee\u5f02\u68c0\u9a8c\u65b9\u6cd5\u54e6\uff01wilcox.test\u4ee3\u8868\u662fWilcox\u79e9\u548c\u68c0\u9a8c\uff0c\u8fd8\u53ef\u4ee5\u6362\u6210t.test\uff0ckruskal.test\uff0canova\u7b49\u65b9\u5f0f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#Wilcox\u79e9\u548c\u68c0\u9a8c\u662f\u4e00\u79cd\u975e\u53c2\u6570\u65b9\u6cd5\uff0c\u7528\u4e8e\u6bd4\u8f83\u4e24\u7ec4\u6570\u636e\u7684\u5206\u5e03\u5dee\u5f02\uff0c\u7279\u522b\u9002\u7528\u4e8e\u4e0d\u6ee1\u8db3\u6b63\u6001\u5206\u5e03\u5047\u8bbe\u6216\u65b9\u5dee\u9f50\u6027\u5047\u8bbe\u7684\u60c5\u51b5\u3002\u5b83\u5728\u7edf\u8ba1\u5206\u6790\u4e2d\u6709\u5e7f\u6cdb\u7684\u5e94\u7528\uff0c\u7528\u4e8e\u7814\u7a76\u4e24\u7ec4\u6570\u636e\u4e4b\u95f4\u7684\u5dee\u5f02\u3002\u800c\u5176\u4ed6\u7684\u65b9\u6cd5\uff0c\u50cft.test\u5c31\u662fT\u68c0\u9a8c\uff0c\u90fd\u662f\u5927\u540c\u5c0f\u5f02\u7684\uff0c\u5927\u5bb6\u4e5f\u53ef\u4ee5\u81ea\u884c\u9009\u62e9\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd9\u91cc\u5927\u6d77\u54e5\u53ea\u5c55\u793a\u4e00\u5217\u6307\u6807\u7684\u7bb1\u578b\u56fe\uff0c\u7136\u540e\u540c\u6837\u7684\u4ee3\u7801\u5c55\u793a\u53e6\u4e00\u5217\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u53ea\u9700\u8981\u628ay1\u6362\u6210y2\u548cy3\u5c31\u597d\u5566<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggboxplot(rt,<\/span><\/code><code><span class=\"code-snippet_outer\">x=<span class=\"code-snippet__string\">\"group\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">y=y2,<\/span><\/code><code><span class=\"code-snippet_outer\">color=<span class=\"code-snippet__string\">\"group\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">xlab=<span class=\"code-snippet__string\">\"\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">ylab= <span class=\"code-snippet__string\">\" Expression\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">legend.title=<span class=\"code-snippet__string\">\"Type\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">palette = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"#dc0000\"<\/span>,<span class=\"code-snippet__string\">\"#0072b5\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">add = <span class=\"code-snippet__string\">\"jitter\"<\/span>)+ <\/span><\/code><code><span class=\"code-snippet_outer\">stat_compare_means(comparisons = my_comparisons,method = <span class=\"code-snippet__string\">\"wilcox.test\"<\/span>,symnum.args=<span class=\"code-snippet__built_in\">list<\/span>(cutpoints = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__number\">0<\/span>, <span class=\"code-snippet__number\">0.001<\/span>, <span class=\"code-snippet__number\">0.01<\/span>, <span class=\"code-snippet__number\">0.05<\/span>, <span class=\"code-snippet__number\">1<\/span>), symbols = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"***\"<\/span>, <span class=\"code-snippet__string\">\"**\"<\/span>, <span class=\"code-snippet__string\">\"*\"<\/span>, <span class=\"code-snippet__string\">\"-\"<\/span>)),label = <span class=\"code-snippet__string\">\"p.signif\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u770b\u770b\u7ed3\u679c\u5427\uff01<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9457364341085271\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_kpqPcd4cEp5mk8SiaECmrralUvfgHw-3.png\" 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powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px 3px 0px 0px;border-color: rgb(231, 215, 173) rgb(231, 215, 173) rgb(69, 107, 176) rgb(69, 107, 176);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-left: -6px;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px 3px 0px 0px;border-color: rgb(231, 215, 173) rgb(231, 215, 173) rgb(69, 107, 176) rgb(69, 107, 176);\">\n<section 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{"id":53916,"date":"2024-06-16T13:26:02","date_gmt":"2024-06-16T05:26:02","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=53916"},"modified":"2024-06-16T13:26:02","modified_gmt":"2024-06-16T05:26:02","slug":"%e7%bb%84%e9%97%b4%e5%b7%ae%e5%bc%82%e7%ae%b1%e5%9e%8b%e5%9b%be%e6%80%8e%e4%b9%88%e7%94%bb%ef%bc%9f%e5%a4%a7%e6%b5%b7%e5%93%a5%e8%b5%90%e4%bd%a0%e4%bb%a3%e7%a0%81%e6%b3%95%e5%ae%9d%ef%bc%81-3","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=53916","title":{"rendered":"\u7ec4\u95f4\u5dee\u5f02\u7bb1\u578b\u56fe\u600e\u4e48\u753b\uff1f\u5927\u6d77\u54e5\u8d50\u4f60\u4ee3\u7801\u6cd5\u5b9d\uff01"},"content":{"rendered":"

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center;\">\n<section style=\"overflow: hidden;text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: 6px;height: 6px;\">\n<section style=\"transform: rotate(0.1deg);\">\n<section style=\"width: 6px;height: 6px;border-radius: 100%;background-color: rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 100%;margin-right: -6px;margin-left: -7px;border-bottom: 2px dotted rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 6px;height: 6px;border-radius: 100%;background-color: rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.8421052631578947\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_WgT3XTQbJQWicmmCf0S1iarXjJpptA-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSHwEuTm51NEXRqZDxBUdecaX3KofibdS6cTuWxOgpp3Su6THrWgT3XTQbJQWicmmCf0S1iarXjJpptA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"361\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">\u53ef\u4ee5\u770b\u5230\u683c\u5f0f\u975e\u5e38\u7b80\u5355\uff0c\u6bcf\u4e00\u884c\u5bf9\u5e94\u4e00\u4e2a\u6837\u672c\uff0c\u7b2c\u4e00\u5217\u4e3a\u6837\u672c\u540d\uff0c\u7b2c\u4e8c\u5217\u4e3a\u6837\u672c\u5206\u7ec4\uff0c\u540e\u9762\u51e0\u5217\u5c31\u662f\u6211\u4eec\u9700\u8981\u6bd4\u8f83\u7684\u6307\u6807\u4e86\uff0c\u53ef\u4ee5\u662f\u4e00\u5217\u4e5f\u53ef\u4ee5\u662f\u591a\u5217\uff0c\u7136\u540e\u8ba9\u6211\u4eec\u5f00\u59cb\u4ee3\u7801\u90e8\u5206\u5427\uff01<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u52a0\u8f7d\u5173\u952eR\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u52a0\u8f7d\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">rt<-read.table(<span class=\"code-snippet__string\">\"gene_deg.txt\"<\/span>,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,header=<span class=\"code-snippet__built_in\">T<\/span>,check.names=<span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u9884\u89c8\u4e00\u4e0b<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">head(rt)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.34794520547945207\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_hyuRIQJCiaEWrfqmpoDyj1Y2m0iaWA-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSHwEuTm51NEXRqZDxBUdecjRHlIhIZDHbRzG78tn73nn7XkHhyuRIQJCiaEWrfqmpoDyj1Y2m0iaWA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"365\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7136\u540e\u6307\u5b9a\u6211\u4eec\u7684\u5206\u7ec4\u548c\u5bf9\u6bd4\u6307\u6807<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">x=colnames(rt)[<span class=\"code-snippet__number\">2<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">y1=colnames(rt)[<span class=\"code-snippet__number\">3<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">y2=colnames(rt)[<span class=\"code-snippet__number\">4<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">y3=colnames(rt)[<span class=\"code-snippet__number\">5<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5206\u7ec4\u9700\u8981\u8fdb\u884c\u4e00\u4e0b\u56e0\u5b50\u5316\uff0c\u56e0\u4e3a\u662f\u5206\u7c7b\u53d8\u91cf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">group=levels(factor(rt$group))<\/span><\/code><code><span class=\"code-snippet_outer\">rt$group=factor(rt$group,levels=group)<\/span><\/code><code><span class=\"code-snippet_outer\">comp=combn(group,<span class=\"code-snippet__number\">2<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">my_comparisons=<span class=\"code-snippet__built_in\">list<\/span>()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(i <span class=\"code-snippet__keyword\">in<\/span> <span class=\"code-snippet__number\">1<\/span>:ncol(comp)){my_comparisons[[i]]<-comp[,i]}<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\">#\u7136\u540e\u9700\u8981\u8c03\u7528ggpubr\u4e2d\u7684ggboxplot\u65b9\u6cd5\u6765\u8fdb\u884c\u53ef\u89c6\u5316<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> ggboxplot(rt,<\/span><\/code><code><span class=\"code-snippet_outer\">x=<span class=\"code-snippet__string\">\"group\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">y=y1,<\/span><\/code><code><span class=\"code-snippet_outer\">color=<span class=\"code-snippet__string\">\"group\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">xlab=<span class=\"code-snippet__string\">\"\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">ylab= <span class=\"code-snippet__string\">\" Expression\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">legend.title=<span class=\"code-snippet__string\">\"Type\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">palette = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"#dc0000\"<\/span>,<span class=\"code-snippet__string\">\"#0072b5\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">add = <span class=\"code-snippet__string\">\"jitter\"<\/span>)+ <\/span><\/code><code><span class=\"code-snippet_outer\">stat_compare_means(comparisons = my_comparisons,method = <span class=\"code-snippet__string\">\"wilcox.test\"<\/span>,symnum.args=<span class=\"code-snippet__built_in\">list<\/span>(cutpoints = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__number\">0<\/span>, <span class=\"code-snippet__number\">0.001<\/span>, <span class=\"code-snippet__number\">0.01<\/span>, <span class=\"code-snippet__number\">0.05<\/span>, <span class=\"code-snippet__number\">1<\/span>), symbols = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"***\"<\/span>, <span class=\"code-snippet__string\">\"**\"<\/span>, <span class=\"code-snippet__string\">\"*\"<\/span>, <span class=\"code-snippet__string\">\"-\"<\/span>)),label = <span class=\"code-snippet__string\">\"p.signif\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\">#\u8fd9\u91cc\u5927\u6d77\u54e5\u7528*\u53f7\u6765\u4ee3\u66ff\u4e86\u5dee\u5f02\u6027P\u503c\uff0c\u5176\u4e2d\u5c0f\u4e8e1\u5927\u4e8e0.05\u5c31\u662f\u4e00\u4e2a-\uff0c\u7136\u540e\u5c0f\u4e8e0.05\u5927\u4e8e0.01\u5c31\u662f\u4e00\u4e2a*\uff0c\u5c0f\u4e8e0.01\u5927\u4e8e0.001\u5c31\u662f**\uff0c\u5c0f\u4e8e0.001\u5927\u4e8e0\u5c31\u662f***<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5f53\u7136\u4e86\u5927\u5bb6\u4e5f\u53ef\u4ee5\u76f4\u63a5\u663e\u793ap\u503c\u54e6\uff0c\u53ea\u9700\u8981\u628asymnum.args=list(cutpoints = c(0, 0.001, 0.01, 0.05, 1), symbols = c(\"***\", \"**\", \"*\", \"-\")),label = \"p.signif\")\u53bb\u6389\u5c31\u597d\u5566\uff01\u4f1a\u81ea\u52a8\u4ee5\u79d1\u5b66\u8ba1\u6570\u6cd5\u7684\u65b9\u5f0f\u5c55\u793aP\u503c\u54e6\uff01<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\">#\u6211\u4eec\u5148\u770b\u770b*\u53f7\u663e\u793a\u7684\u7ed3\u679c<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9329896907216495\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_a2JuQ9RGyJ2nAej7KWpvMGT7uO4pMg-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSHwEuTm51NEXRqZDxBUdecYwNzoo0qYTJZOgUJ32IKrFiaUia2JuQ9RGyJ2nAej7KWpvMGT7uO4pMg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"388\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u770b\u7740\u662f\u4e0d\u662f\u5f88\u4e0d\u9519<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6211\u4eec\u518d\u770b\u770b\u76f4\u63a5\u663e\u793aP\u503c\u7684<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9692307692307692\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_abYVUkW50P9ic7CXfXvgPuYDZYnC9g-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSHwEuTm51NEXRqZDxBUdecyIXGIQCib0ddz7YqqZ3Oib1tAEJabYVUkW50P9ic7CXfXvgPuYDZYnC9g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"390\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u800c\u4e14stat_compare_means\u4e2d\u7684method\u53ef\u4ee5\u6307\u5b9a\u4e0d\u540c\u7684\u5dee\u5f02\u68c0\u9a8c\u65b9\u6cd5\u54e6\uff01wilcox.test\u4ee3\u8868\u662fWilcox\u79e9\u548c\u68c0\u9a8c\uff0c\u8fd8\u53ef\u4ee5\u6362\u6210t.test\uff0ckruskal.test\uff0canova\u7b49\u65b9\u5f0f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#Wilcox\u79e9\u548c\u68c0\u9a8c\u662f\u4e00\u79cd\u975e\u53c2\u6570\u65b9\u6cd5\uff0c\u7528\u4e8e\u6bd4\u8f83\u4e24\u7ec4\u6570\u636e\u7684\u5206\u5e03\u5dee\u5f02\uff0c\u7279\u522b\u9002\u7528\u4e8e\u4e0d\u6ee1\u8db3\u6b63\u6001\u5206\u5e03\u5047\u8bbe\u6216\u65b9\u5dee\u9f50\u6027\u5047\u8bbe\u7684\u60c5\u51b5\u3002\u5b83\u5728\u7edf\u8ba1\u5206\u6790\u4e2d\u6709\u5e7f\u6cdb\u7684\u5e94\u7528\uff0c\u7528\u4e8e\u7814\u7a76\u4e24\u7ec4\u6570\u636e\u4e4b\u95f4\u7684\u5dee\u5f02\u3002\u800c\u5176\u4ed6\u7684\u65b9\u6cd5\uff0c\u50cft.test\u5c31\u662fT\u68c0\u9a8c\uff0c\u90fd\u662f\u5927\u540c\u5c0f\u5f02\u7684\uff0c\u5927\u5bb6\u4e5f\u53ef\u4ee5\u81ea\u884c\u9009\u62e9\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd9\u91cc\u5927\u6d77\u54e5\u53ea\u5c55\u793a\u4e00\u5217\u6307\u6807\u7684\u7bb1\u578b\u56fe\uff0c\u7136\u540e\u540c\u6837\u7684\u4ee3\u7801\u5c55\u793a\u53e6\u4e00\u5217\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u53ea\u9700\u8981\u628ay1\u6362\u6210y2\u548cy3\u5c31\u597d\u5566<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggboxplot(rt,<\/span><\/code><code><span class=\"code-snippet_outer\">x=<span class=\"code-snippet__string\">\"group\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">y=y2,<\/span><\/code><code><span class=\"code-snippet_outer\">color=<span class=\"code-snippet__string\">\"group\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">xlab=<span class=\"code-snippet__string\">\"\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">ylab= <span class=\"code-snippet__string\">\" Expression\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">legend.title=<span class=\"code-snippet__string\">\"Type\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">palette = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"#dc0000\"<\/span>,<span class=\"code-snippet__string\">\"#0072b5\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">add = <span class=\"code-snippet__string\">\"jitter\"<\/span>)+ <\/span><\/code><code><span class=\"code-snippet_outer\">stat_compare_means(comparisons = my_comparisons,method = <span class=\"code-snippet__string\">\"wilcox.test\"<\/span>,symnum.args=<span class=\"code-snippet__built_in\">list<\/span>(cutpoints = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__number\">0<\/span>, <span class=\"code-snippet__number\">0.001<\/span>, <span class=\"code-snippet__number\">0.01<\/span>, <span class=\"code-snippet__number\">0.05<\/span>, <span class=\"code-snippet__number\">1<\/span>), symbols = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"***\"<\/span>, <span class=\"code-snippet__string\">\"**\"<\/span>, <span class=\"code-snippet__string\">\"*\"<\/span>, <span class=\"code-snippet__string\">\"-\"<\/span>)),label = <span class=\"code-snippet__string\">\"p.signif\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u770b\u770b\u7ed3\u679c\u5427\uff01<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9457364341085271\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_kpqPcd4cEp5mk8SiaECmrralUvfgHw-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSHwEuTm51NEXRqZDxBUdec4u4vqt4w4VdMXY4kbhbVbOVXakpqPcd4cEp5mk8SiaECmrralUvfgHw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"387\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">#\u662f\u4e0d\u662f\u5f88\u7b80\u5355\uff0c\u518d\u591a\u7684\u6307\u6807\u4e5f\u662f\u901a\u8fc7\u4fee\u6539y\u5c31\u597d\u4e86\uff0c\u7136\u540e\u6211\u4eec\u518d\u624b\u52a8\u6574\u5408\u4e00\u4e0b\u7ed3\u679c\u5c31\u53ef\u4ee5\u76f4\u63a5\u653e\u5230\u8bba\u6587\u91cc\u4e86\uff0c\u7b80\u5355\u53c8\u597d\u7528\u8fd8\u597d\u770b\uff0c\u8bf4\u662f\u6cd5\u5b9d\u4e0d\u4e3a\u8fc7\uff0c\u53ea\u5e0c\u671b\u5bf9\u5c0f\u4f19\u4f34\u4eec\u6709\u7528\u5c31\u597d\u3002<\/p>\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"box-shadow: rgb(170, 170, 170) 0px 0px 10px inset;border-color: rgb(192, 200, 209);padding: 10px;border-width: 1px;border-style: solid;background-color: rgb(250, 250, 239);\">\n<section style=\"color: rgb(51, 51, 51);text-align: center;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p style=\"text-align: justify;text-wrap: 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