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justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\">1.CIBERSORT\u7b97\u6cd5\u4ecb\u7ecd<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: flex-end;\" mpa-from-tpl=\"t\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section 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underline;\">\u516c\u4f17\u53f7\u540e\u53f0\u56de\u590d\u201c111\u201d\u9886\u53d6\u4ee3\u7801\uff0c\u4ee3\u7801\u7f16\u53f7\uff1a231007<\/span><\/span><\/em><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3888888888888889\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_IlmBAWe69EMUPMhNEgrv6NXX85lJxg-13.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAwyrj7kbUR3ULG9ZUbLrLkgibzL8qUpQROfe8ebc3gNSnu1dIlmBAWe69EMUPMhNEgrv6NXX85lJxg\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"\" \/><\/p>\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\">2.\u51c6\u5907\u9700\u8981\u7684R\u5305<\/strong><em style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;text-align: center;letter-spacing: 0.034em;\"><\/em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.034em;text-align: left;\"><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<h2 style=\"text-align: center;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/h2>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"sql\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5b89\u88c5\u9700\u8981\u7684R\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"ggplot2\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"reshape2\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"ggpubr\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"dplyr\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"ggsci\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">BiocManager::<span class=\"code-snippet__keyword\">install<\/span>(<span class=\"code-snippet__string\">\"RcolorBrewer\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u52a0\u8f7d\u9700\u8981\u7684R\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">library<\/span>(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">library<\/span>(reshape2)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">library<\/span>(ggpubr)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">library<\/span>(dplyr)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">library<\/span>(RcolorBrewer)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">source<\/span>(<span class=\"code-snippet__string\">'Cibersort.R'<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\">3.\u8bfb\u53d6\u6587\u4ef6<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: flex-end;\" mpa-from-tpl=\"t\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"transform: translate3d(5px, 0px, 0px) rotateX(180deg);\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 460px;background-color: rgb(244, 171, 189);\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-align: center;\"><span style=\"font-size: 20px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><em><strong><span style=\"font-size: 20px;\"><\/span><\/strong><\/em><\/span><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><span style=\"font-family:Calibri;\">#<\/span><\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\">LM22.txt<\/span><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><span style=\"font-family:Calibri;\">,22<\/span><span style=\"font-family:\u5b8b\u4f53;\">\u79cd\u514d\u75ab\u7ec6\u80de\u8868\u8fbe\u91cf\u6587\u4ef6\uff0c\u7b2c\u4e00\u5217\u4e3a<\/span><span style=\"font-family:Calibri;\">Gene symbol,<\/span><span style=\"font-family:\u5b8b\u4f53;\">\u5176\u4ed6\u5217\u4e3a<\/span><span style=\"font-family:Calibri;\">22<\/span><span style=\"font-family:\u5b8b\u4f53;\">\u79cd\u514d\u75ab\u7ec6\u80de\u3002<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">LM22<\/span><span class=\"code-snippet__selector-class\">.file<\/span> <<span class=\"code-snippet__selector-tag\">-<\/span> \"<span class=\"code-snippet__selector-tag\">LM22<\/span><span class=\"code-snippet__selector-class\">.txt<\/span>\"<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.20694645441389292\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_HFVlHiaNt42ibYNRS5lfpvgK8ZxHvA-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyBMiaebYV0wRXKtNfxorecdLOEUylUANF4abd9kABgS3vdmPNHFVlHiaNt42ibYNRS5lfpvgK8ZxHvA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"691\" style=\"\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><span style=\"font-family:Calibri;\">#combined.expr.txt<\/span><span style=\"font-family:\u5b8b\u4f53;\">\uff0c\u8868\u8fbe\u77e9\u9635\u6587\u4ef6\uff0c\u884c\u540d\u4e3a\u57fa\u56e0\u540d\uff0c\u5217\u540d\u4e3a\u6837\u672c\u4fe1\u606f\u3002<\/span><\/span><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">GEO_exp<\/span><span class=\"code-snippet__selector-class\">.file<\/span> <<span class=\"code-snippet__selector-tag\">-<\/span> \"<span class=\"code-snippet__selector-tag\">combined<\/span><span class=\"code-snippet__selector-class\">.expr<\/span><span class=\"code-snippet__selector-class\">.txt<\/span>\"<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.25722543352601157\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_dsogU7bA8KtS3uhicFtFjFapgiaevQ-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyBMiaebYV0wRXKtNfxorecdMj3icPtRmehiaj5yJJbr7ND8ATx8dsogU7bA8KtS3uhicFtFjFapgiaevQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"692\" style=\"\" \/><\/p>\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\">4.CIBERSORT\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: flex-end;\" mpa-from-tpl=\"t\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"transform: translate3d(5px, 0px, 0px) rotateX(180deg);\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 460px;background-color: rgb(244, 171, 189);\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><em style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;text-align: center;letter-spacing: 0.034em;\"><\/em><span style=\"font-family: \u5b8b\u4f53;font-size: 10.5pt;text-align: center;letter-spacing: 0.034em;\"><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"objectivec\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#perm\u8868\u793a\u7f6e\u6362\u6b21\u6570, QN\u5982\u679c\u662f\u82af\u7247\u8bbe\u7f6e\u4e3aT\uff0c\u5982\u679c\u662f\u6d4b\u5e8f\u5c31\u8bbe\u7f6e\u4e3aF<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">GEO_cibersort.results <- <span class=\"code-snippet__built_in\">CIBERSORT<\/span>(LM22.file ,GEO_exp.file, perm = <span class=\"code-snippet__number\">50<\/span>, QN = T)<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(GEO_cibersort.results, <span class=\"code-snippet__string\">\"GEO_CIBERSORT.txt\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u8ba1\u7b97\u768422\u79cd\u514d\u75ab\u7ec6\u80de\u6bd4\u4f8b\u7ed3\u679c\u6587\u4ef6\uff0c\u884c\u540d\u4e3a\u6837\u672c\u4fe1\u606f\uff0c\u5217\u540d\u4e3a\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\u3002<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2691751085383502\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_GyqAx3OL3BKgLvLjmoWEQpUJCibK9A-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyBMiaebYV0wRXKtNfxorecdhmiaice2bGG7gz1VMYccTcn6icQhGyqAx3OL3BKgLvLjmoWEQpUJCibK9A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"691\" style=\"\" \/><\/p>\n<p style=\"margin-left: 0pt;text-align: center;\"><span style=\"font-size: 20px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><em><strong><span style=\"font-size: 20px;\"><span style=\"font-size: 20px;\"><\/span><\/span><\/strong><\/em><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\"># \u63d0\u53d6cibersort\u524d22\u5217\u6570\u636e\uff0c23-25\u5217\u4e3a P-value\uff0c P-value\uff0cRMSE\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort_data <- <span class=\"code-snippet__keyword\">as<\/span>.data.frame(GEO_cibersort.results[,<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">22<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5c06\u884c\u540d\u8f6c\u5316\u4e3a\u5217\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort_data<-rownames_to_column(cibersort_data,<span class=\"code-snippet__keyword\">var<\/span>=<span class=\"code-snippet__string\">\"Sample\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u8bfb\u53d6\u6837\u672c\u5206\u7ec4\u6587\u4ef6,\u5305\u62ec\u4e24\u5217\u4fe1\u606f\uff0c\u7b2c\u4e00\u5217\u4e3a\u6837\u672c\u540d\uff0c\u7b2c\u4e8c\u5217\u4e3a\u5206\u7ec4\u4fe1\u606f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">Group<-read.table(\u201c<span class=\"code-snippet__keyword\">group<\/span>.txt\u201d,header=T,sep=\u201dt\u201d)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2615606936416185\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_JjicGx2pIcRThLSzOZib8T1HVt437Q-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyBMiaebYV0wRXKtNfxorecdqAHtkYDQGpphHiauJSvoKsaK733JjicGx2pIcRThLSzOZib8T1HVt437Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"692\" style=\"\" \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><br \/><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u8fde\u63a5\u5206\u7ec4\u4fe1\u606f\u548ccibersort\u7ed3\u679c\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort<-left_join(cibersort_data,Group,<span class=\"code-snippet__keyword\">by<\/span>=<span class=\"code-snippet__string\">\"Sample\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u957f\u5bbd\u6570\u636e\u8f6c\u6362<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort<- melt(cibersort,id.vars=c(<span class=\"code-snippet__string\">\"Sample\"<\/span>,<span class=\"code-snippet__string\">\"group\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u8bbe\u7f6e\u884c\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">colnames(cibersort)<-c(<span class=\"code-snippet__string\">\"Sample\"<\/span>,<span class=\"code-snippet__string\">\"Group\"<\/span>,<span class=\"code-snippet__string\">\"celltype\"<\/span>,<span class=\"code-snippet__string\">\"composition\"<\/span>) <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u7ed8\u5236\u5dee\u5f02\u5206\u6790\u7bb1\u7ebf\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">boxplot_cibersort<- ggplot(cibersort, aes(x = celltype, y = composition))+ <\/span><\/code><code><span class=\"code-snippet_outer\"> labs(y=<span class=\"code-snippet__string\">\"Cell composition\"<\/span>,x= <span class=\"code-snippet__string\">\"\"<\/span>)+ <\/span><\/code><code><span class=\"code-snippet_outer\"> geom_boxplot(aes(fill = Group),position=position_dodge(<span class=\"code-snippet__number\">0.5<\/span>),width=<span class=\"code-snippet__number\">0.5<\/span>)+ <\/span><\/code><code><span class=\"code-snippet_outer\"> scale_fill_npg()+<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__meta\">#\u4fee\u6539\u4e3b\u9898<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">theme_bw() + <\/span><\/code><code><span class=\"code-snippet_outer\"> theme(axis.title = element_text(size = <span class=\"code-snippet__number\">12<\/span>,color =<span class=\"code-snippet__string\">\"black\"<\/span>), <\/span><\/code><code><span class=\"code-snippet_outer\"> axis.text = element_text(size= <span class=\"code-snippet__number\">12<\/span>,color = <span class=\"code-snippet__string\">\"black\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> panel.grid.minor.y = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> panel.grid.minor.x = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.text.x = element_text(angle = <span class=\"code-snippet__number\">45<\/span>, hjust = <span class=\"code-snippet__number\">1<\/span> ),<\/span><\/code><code><span class=\"code-snippet_outer\"> panel.grid=element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.position = <span class=\"code-snippet__string\">\"top\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.text = element_text(size= <span class=\"code-snippet__number\">12<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.title= element_text(size= <span class=\"code-snippet__number\">12<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> ) +<\/span><\/code><code><span class=\"code-snippet_outer\"> stat_compare_means(aes(<span class=\"code-snippet__keyword\">group<\/span> = Group),<\/span><\/code><code><span class=\"code-snippet_outer\"> label = <span class=\"code-snippet__string\">\"p.signif\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> method = <span class=\"code-snippet__string\">\"wilcox.test\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> hide.ns = T)<span class=\"code-snippet__meta\">#\u9690\u85cf\u4e0d\u663e\u8457\u7684<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggsave(file=<span class=\"code-snippet__string\">\"cibersort.pdf\"<\/span>,boxplot_cibersort,height=<span class=\"code-snippet__number\">10<\/span>,width=<span class=\"code-snippet__number\">15<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6628664495114006\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_26kI3vxBCOP8UVuAb6KKoRCyDwAyCg-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyBMiaebYV0wRXKtNfxorecdlA8xUHjibok7D66XuaQ2BA6PH26kI3vxBCOP8UVuAb6KKoRCyDwAyCg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"614\" style=\"\" \/><\/p>\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\"><strong>5<\/strong><strong>.\u7ed8\u5236\u514d\u75ab\u7ec6\u80de\u542b\u91cf\u67f1\u72b6\u56fe<\/strong><\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: flex-end;\" mpa-from-tpl=\"t\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"transform: translate3d(5px, 0px, 0px) rotateX(180deg);\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 460px;background-color: rgb(244, 171, 189);\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><em style=\"text-align: center;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;letter-spacing: 0.034em;\"><\/em><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u957f\u5bbd\u6570\u636e\u8f6c\u6362<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort_plot<-melt(cibersort_data,id.vars=\u201dSample\u201d)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4fee\u6539\u5217\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">colnames(cibersort_plot)<-c(<span class=\"code-snippet__string\">\"Sample\"<\/span>,<span class=\"code-snippet__string\">\"\"<\/span>celltype<span class=\"code-snippet__string\">\",\"<\/span>composition<span class=\"code-snippet__string\">\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">#\u7ed8\u5236\u514d\u75ab\u7ec6\u80de\u542b\u91cf\u5806\u53e0\u67f1\u72b6\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\">##\u81ea\u5b9a\u4e49\u989c\u8272\u8bbe\u7f6e<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet_outer\">colour = c(brewer.pal(12, \"<\/span>Paired<span class=\"code-snippet__string\">\"),brewer.pal(8, \"<\/span>Dark2<span class=\"code-snippet__string\">\"),brewer.pal(12, \"<\/span>Set3<span class=\"code-snippet__string\">\"))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggplot(data=cibersort_plot,aes(x=Sample,y=composition,fill=celltype))+<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet_outer\"> geom_bar(position=\"<\/span>stack<span class=\"code-snippet__string\">\",stat=\"<\/span>identity<span class=\"code-snippet__string\">\")+ #\u7ed8\u5236\u67f1\u72b6\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> scale_fill_manual(values = colour)+ #\u6dfb\u52a0\u81ea\u5b9a\u4e49\u989c\u8272<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet_outer\"> labs(x=\"<\/span><span class=\"code-snippet__string\">\",y=\"<\/span><span class=\"code-snippet__string\">\",title=\"<\/span>cell proportion<span class=\"code-snippet__string\">\")+ #\u8bbe\u7f6e\u5750\u6807\u8f74<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> scale_y_continuous(expand=c(0,0))+ #\u8bbe\u7f6e\u4e0ey\u8f74\u7684\u95f4\u8ddd\u4e3a0<\/span><\/code><code><span class=\"code-snippet_outer\"> guides(fill = guide_legend(ncol = 1))+ #\u8bbe\u7f6e\u56fe\u4f8b\u6392\u5217\u6210\u4e00\u5217<\/span><\/code><code><span class=\"code-snippet_outer\"> #\u4fee\u6539\u4e3b\u9898<\/span><\/code><code><span class=\"code-snippet_outer\">theme_bw()+<\/span><\/code><code><span class=\"code-snippet_outer\"> theme(legend.key = element_blank(),legend.title = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> panel.grid=element_blank(), <\/span><\/code><code><span class=\"code-snippet_outer\"> axis.text.x=element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.ticks.x=element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> plot.title=element_text(hjust=0.5))<\/span><\/code><code><span class=\"code-snippet_outer\">#\u4fdd\u5b58\u56fe\u7247<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet_outer\">ggsave(\"<\/span>cibersort_barplot.pdf<span class=\"code-snippet__string\">\",width=8,height=8)<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9859437751004017\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_IDOibQKaZ2icgU7t2ynhn37R8Rzicg-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyBMiaebYV0wRXKtNfxorecdia1iaLHibpLREUy0MdZmFiaMlJ3W4sMIDOibQKaZ2icgU7t2ynhn37R8Rzicg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"498\" style=\"\" \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u6700\u7ec8\u5c0f\u679c\u6210\u529f\u7684\u5229\u7528CIBERSORT\u7b97\u6cd5\u8fdb\u884c\u4e86\u514d\u75ab\u6d78\u6da6\u5206\u6790\uff0c\u7ed8\u5236\u4e86\u514d\u75ab\u7ec6\u80de\u4e30\u5ea6\u5806\u53e0\u67f1\u72b6\u56fe\u548c\u5206\u7ec4\u5dee\u5f02\u5206\u6790\u7bb1\u7ebf\u56fe\uff0c\u770b\u8d77\u6765\u56fe\u7247\u6548\u679c\u975e\u5e38\u4e0d\u9519\uff0c\u6b22\u8fce\u5927\u5bb6\u548c\u5c0f\u679c\u4e00\u8d77\u8ba8\u8bba\u5b66\u4e60\u5440\uff01<\/span><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4eca\u5929\u5c0f\u679c\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\uff0c\u4e0b\u671f\u5728\u89c1\u5965\u3002<\/span><\/strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><\/span><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;text-align: left;\">\n<section style=\"font-size: 16px;\">\n<section powered-by=\"xiumi.us\" style=\"margin: 10px;justify-content: flex-start;display: flex;flex-flow: row;\">\n<section style=\"padding-right: 10px;padding-left: 10px;display: inline-block;width: 558px;vertical-align: top;border-style: dashed;border-width: 1px;border-color: rgb(62, 62, 62);align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;width: 536px;border-width: 0px 0px 0px 10px;border-style: solid;border-left-color: rgb(144, 215, 236);border-right-color: rgb(46, 172, 109);box-shadow: rgb(0, 0, 0) 0px 0px 0px;border-bottom-left-radius: 0px;\">\n<section powered-by=\"xiumi.us\" style=\"padding-right: 3px;padding-left: 3px;\">\n<section style=\"margin-top: 0.95em;margin-bottom: -0.65em;display: inline-block;width: 520px;vertical-align: top;\">\n<section style=\"width: 520px;\">\n<section style=\"margin-top: -3px;width: 6px;height: 6px;border-radius: 100%;float: right;background-color: rgb(46, 172, 109);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: -1em;padding: 3px 10px;display: inline-block;vertical-align: top;background-color: rgb(255, 255, 255);color: rgb(0, 154, 68);\">\n<p><strong>\u5f80\u671f\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: justify;\">\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491794&idx=1&sn=2b7a4de95169c79202e44813885a8250&chksm=cf7f7017f808f9015689839fe4057fd418bf7b20eb1dfb5a28cd92e56a41b5605e62bfbc16fb&scene=21#wechat_redirect\" textvalue=\"1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\"><span style=\"font-size: 14px;\">1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4<\/span><\/a><br \/><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491705&idx=1&sn=9ff9b6da28b3a256a99c6697d589be66&chksm=cf7f70bcf808f9aafd1019a0bba3dd3a55e2867dbb9a09203f13c2683adf867094637c405ed1&scene=21#wechat_redirect\" textvalue=\"2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\"><span style=\"font-size: 14px;\">2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86<\/span><\/a><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491909&idx=1&sn=902e34bcf53e605fba2b457ec6a33e36&chksm=cf7f7180f808f8961542f458a49da14e85d8831449fb65fbf2623d55affc564686a5422f56fd&scene=21#wechat_redirect\" textvalue=\"3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\"><span style=\"font-size: 14px;\">3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8<\/span><\/a><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491905&idx=1&sn=fb34ebd6282962c7803badaee7a39096&chksm=cf7f7184f808f8929608c0050344ad86193d0fc6455c1c18e16cc17bf4b37832b63d3b18284e&scene=21#wechat_redirect\" textvalue=\"4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\"><span style=\"font-size: 14px;\">4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b<\/span><\/a><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;text-align: left;\"><span style=\"font-size: 10.5pt;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247493633&idx=1&sn=2263dfe7572c0bcf6816c2c1a37d79de&chksm=cf7f78c4f808f1d2209321d6ace39fde5fa60c0691b56f88eac21916f5a2872ce8ee345f1d5f&scene=21#wechat_redirect\" textvalue=\"\u4f60\u5df2\u9009\u4e2d\u4e86\u6dfb\u52a0\u94fe\u63a5\u7684\u5185\u5bb9\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.4255555555555556\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_3enDMf2LD6pjia3zQolKXIfTwuh6AA-13.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxweqRKIWbRUBSWSGID9aog3q3QVgsHZplb1JyboXh8CPz6b3enDMf2LD6pjia3zQolKXIfTwuh6AA\/640?wx_fmt=jpeg&wxfrom=5&wx_lazy=1&wx_co=1'\" data-w=\"900\"><\/span><\/a><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3888888888888889\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_SibFRUHIk0cCbShYgOsGDPrxeHkEtw-13.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAwyrj7kbUR3ULG9ZUbLrLkgvrEB1jg0GibYczZiaOORjzpxpIjSibFRUHIk0cCbShYgOsGDPrxeHkEtw\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"\"><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u9ad8\u5206\u751f\u4fe1\u6587\u7ae0\u6700\u53d7\u6b22\u8fce\u7684\u514d\u75ab\u6d78\u6da6\u5206\u6790\u7b97\u6cd5-CIBERSORT \u539f\u521b \u5c0f\u679c &nb 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style=\"transform: translate3d(5px, 0px, 0px) rotateX(180deg);\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 460px;background-color: rgb(244, 171, 189);\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><br mpa-from-tpl=\"t\" \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-align: justify;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.034em;text-align: justify;\">\u5c0f\u679c\u6765\u4e3a\u5c0f\u4f19\u4f34\u4eec\u7b80\u5355\u4ecb\u7ecd\u4e00\u4e0bCIBERSORT\u7b97\u6cd5\uff0cCIBERSORT\u5229\u7528\u7ebf\u6027\u652f\u6301\u5411\u91cf\u56de\u5f52\u7684\u539f\u7406\u5bf9\u514d\u75ab\u7ec6\u80de\u4e9a\u578b\u7684\u8868\u8fbe\u77e9\u9635\u8fdb\u884c\u53bb\u5377\u79ef\uff0c\u6765\u4f30\u8ba1\u514d\u75ab\u7ec6\u80de\u7684\u4e30\u5ea6\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.034em;text-align: justify;\">CIBERSORT\u63d0\u4f9b\u4e8622\u79cd\u5e38\u89c1\u7684\u514d\u75ab\u6d78\u6da6\u7ec6\u80de\u8868\u8fbe\u6570\u636eLM22\uff0c\u5305\u62ec\u4e0d\u540c\u7684\u7ec6\u80de\u7c7b\u578b\u548c\u529f\u80fd\u72b6\u6001\u7684\u514d\u75ab\u7ec6\u80de\uff0c\u8fd9\u5c31\u662f\u5c0f\u679c\u5bf9\u8be5\u7b97\u6cd5\u7684\u4ecb\u7ecd\uff0c\u5c0f\u4f19\u4f34\u4eec\u6709\u6ca1\u6709\u7406\u89e3\u5440\uff01<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.034em;text-align: justify;\">\u5229\u7528CIBERSORT\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u5206\u6790\uff0c\u9700\u8981\u5728\u5b98\u7f51\u4e0b\u8f7d22\u79cd\u5e38\u89c1\u7684\u514d\u75ab\u6d78\u6da6\u7ec6\u80de\u8868\u8fbe\u6570\u636eLM22\u6587\u4ef6\uff0c\u4ee5\u53ca\u51c6\u5907\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u6587\u4ef6\u5c31\u53ef\u4ee5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790\uff0c\u975e\u5e38\u7b80\u5355\uff0c\u5f88\u9002\u5408\u5c0f\u767d\u5b66\u4e60\uff0c\u63a5\u4e0b\u6765\u8ddf\u7740\u5c0f\u679c\u5f00\u59cb\u4eca\u5929\u7684\u5b9e\u64cd\u5427\uff01<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"color: rgb(178, 178, 178);\"><em><span style=\"font-family:Helvetica Neue, Helvetica, Hiragino Sans GB, Microsoft YaHei, Arial, sans-serif;\"><span style=\"color: rgb(178, 178, 178);font-size: 14px;letter-spacing: 0.476px;text-decoration: underline;\">\u516c\u4f17\u53f7\u540e\u53f0\u56de\u590d\u201c111\u201d\u9886\u53d6\u4ee3\u7801\uff0c\u4ee3\u7801\u7f16\u53f7\uff1a231007<\/span><\/span><\/em><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3888888888888889\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_IlmBAWe69EMUPMhNEgrv6NXX85lJxg-13.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAwyrj7kbUR3ULG9ZUbLrLkgibzL8qUpQROfe8ebc3gNSnu1dIlmBAWe69EMUPMhNEgrv6NXX85lJxg\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"\" \/><\/p>\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\">2.\u51c6\u5907\u9700\u8981\u7684R\u5305<\/strong><em style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;text-align: center;letter-spacing: 0.034em;\"><\/em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.034em;text-align: left;\"><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<h2 style=\"text-align: center;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/h2>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"sql\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5b89\u88c5\u9700\u8981\u7684R\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"ggplot2\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"reshape2\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"ggpubr\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"dplyr\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"ggsci\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">BiocManager::<span class=\"code-snippet__keyword\">install<\/span>(<span class=\"code-snippet__string\">\"RcolorBrewer\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u52a0\u8f7d\u9700\u8981\u7684R\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">library<\/span>(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">library<\/span>(reshape2)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">library<\/span>(ggpubr)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">library<\/span>(dplyr)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">library<\/span>(RcolorBrewer)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">source<\/span>(<span class=\"code-snippet__string\">'Cibersort.R'<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\">3.\u8bfb\u53d6\u6587\u4ef6<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: flex-end;\" mpa-from-tpl=\"t\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"transform: translate3d(5px, 0px, 0px) rotateX(180deg);\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 460px;background-color: rgb(244, 171, 189);\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-align: center;\"><span style=\"font-size: 20px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><em><strong><span style=\"font-size: 20px;\"><\/span><\/strong><\/em><\/span><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><span style=\"font-family:Calibri;\">#<\/span><\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\">LM22.txt<\/span><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><span style=\"font-family:Calibri;\">,22<\/span><span style=\"font-family:\u5b8b\u4f53;\">\u79cd\u514d\u75ab\u7ec6\u80de\u8868\u8fbe\u91cf\u6587\u4ef6\uff0c\u7b2c\u4e00\u5217\u4e3a<\/span><span style=\"font-family:Calibri;\">Gene symbol,<\/span><span style=\"font-family:\u5b8b\u4f53;\">\u5176\u4ed6\u5217\u4e3a<\/span><span style=\"font-family:Calibri;\">22<\/span><span style=\"font-family:\u5b8b\u4f53;\">\u79cd\u514d\u75ab\u7ec6\u80de\u3002<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">LM22<\/span><span class=\"code-snippet__selector-class\">.file<\/span> <<span class=\"code-snippet__selector-tag\">-<\/span> \"<span class=\"code-snippet__selector-tag\">LM22<\/span><span class=\"code-snippet__selector-class\">.txt<\/span>\"<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.20694645441389292\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_HFVlHiaNt42ibYNRS5lfpvgK8ZxHvA-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyBMiaebYV0wRXKtNfxorecdLOEUylUANF4abd9kABgS3vdmPNHFVlHiaNt42ibYNRS5lfpvgK8ZxHvA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"691\" style=\"\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><span style=\"font-family:Calibri;\">#combined.expr.txt<\/span><span style=\"font-family:\u5b8b\u4f53;\">\uff0c\u8868\u8fbe\u77e9\u9635\u6587\u4ef6\uff0c\u884c\u540d\u4e3a\u57fa\u56e0\u540d\uff0c\u5217\u540d\u4e3a\u6837\u672c\u4fe1\u606f\u3002<\/span><\/span><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">GEO_exp<\/span><span class=\"code-snippet__selector-class\">.file<\/span> <<span class=\"code-snippet__selector-tag\">-<\/span> \"<span class=\"code-snippet__selector-tag\">combined<\/span><span class=\"code-snippet__selector-class\">.expr<\/span><span class=\"code-snippet__selector-class\">.txt<\/span>\"<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.25722543352601157\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_dsogU7bA8KtS3uhicFtFjFapgiaevQ-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyBMiaebYV0wRXKtNfxorecdMj3icPtRmehiaj5yJJbr7ND8ATx8dsogU7bA8KtS3uhicFtFjFapgiaevQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"692\" style=\"\" \/><\/p>\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\">4.CIBERSORT\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: flex-end;\" mpa-from-tpl=\"t\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"transform: translate3d(5px, 0px, 0px) rotateX(180deg);\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 460px;background-color: rgb(244, 171, 189);\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><em style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;text-align: center;letter-spacing: 0.034em;\"><\/em><span style=\"font-family: \u5b8b\u4f53;font-size: 10.5pt;text-align: center;letter-spacing: 0.034em;\"><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"objectivec\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#perm\u8868\u793a\u7f6e\u6362\u6b21\u6570, QN\u5982\u679c\u662f\u82af\u7247\u8bbe\u7f6e\u4e3aT\uff0c\u5982\u679c\u662f\u6d4b\u5e8f\u5c31\u8bbe\u7f6e\u4e3aF<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">GEO_cibersort.results <- <span class=\"code-snippet__built_in\">CIBERSORT<\/span>(LM22.file ,GEO_exp.file, perm = <span class=\"code-snippet__number\">50<\/span>, QN = T)<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(GEO_cibersort.results, <span class=\"code-snippet__string\">\"GEO_CIBERSORT.txt\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u8ba1\u7b97\u768422\u79cd\u514d\u75ab\u7ec6\u80de\u6bd4\u4f8b\u7ed3\u679c\u6587\u4ef6\uff0c\u884c\u540d\u4e3a\u6837\u672c\u4fe1\u606f\uff0c\u5217\u540d\u4e3a\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\u3002<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2691751085383502\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_GyqAx3OL3BKgLvLjmoWEQpUJCibK9A-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyBMiaebYV0wRXKtNfxorecdhmiaice2bGG7gz1VMYccTcn6icQhGyqAx3OL3BKgLvLjmoWEQpUJCibK9A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"691\" style=\"\" \/><\/p>\n<p style=\"margin-left: 0pt;text-align: center;\"><span style=\"font-size: 20px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><em><strong><span style=\"font-size: 20px;\"><span style=\"font-size: 20px;\"><\/span><\/span><\/strong><\/em><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\"># \u63d0\u53d6cibersort\u524d22\u5217\u6570\u636e\uff0c23-25\u5217\u4e3a P-value\uff0c P-value\uff0cRMSE\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort_data <- <span class=\"code-snippet__keyword\">as<\/span>.data.frame(GEO_cibersort.results[,<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">22<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5c06\u884c\u540d\u8f6c\u5316\u4e3a\u5217\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort_data<-rownames_to_column(cibersort_data,<span class=\"code-snippet__keyword\">var<\/span>=<span class=\"code-snippet__string\">\"Sample\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u8bfb\u53d6\u6837\u672c\u5206\u7ec4\u6587\u4ef6,\u5305\u62ec\u4e24\u5217\u4fe1\u606f\uff0c\u7b2c\u4e00\u5217\u4e3a\u6837\u672c\u540d\uff0c\u7b2c\u4e8c\u5217\u4e3a\u5206\u7ec4\u4fe1\u606f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">Group<-read.table(\u201c<span class=\"code-snippet__keyword\">group<\/span>.txt\u201d,header=T,sep=\u201dt\u201d)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2615606936416185\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_JjicGx2pIcRThLSzOZib8T1HVt437Q-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyBMiaebYV0wRXKtNfxorecdqAHtkYDQGpphHiauJSvoKsaK733JjicGx2pIcRThLSzOZib8T1HVt437Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"692\" style=\"\" \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><br \/><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u8fde\u63a5\u5206\u7ec4\u4fe1\u606f\u548ccibersort\u7ed3\u679c\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort<-left_join(cibersort_data,Group,<span class=\"code-snippet__keyword\">by<\/span>=<span class=\"code-snippet__string\">\"Sample\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u957f\u5bbd\u6570\u636e\u8f6c\u6362<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort<- melt(cibersort,id.vars=c(<span class=\"code-snippet__string\">\"Sample\"<\/span>,<span class=\"code-snippet__string\">\"group\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u8bbe\u7f6e\u884c\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">colnames(cibersort)<-c(<span class=\"code-snippet__string\">\"Sample\"<\/span>,<span class=\"code-snippet__string\">\"Group\"<\/span>,<span class=\"code-snippet__string\">\"celltype\"<\/span>,<span class=\"code-snippet__string\">\"composition\"<\/span>) <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u7ed8\u5236\u5dee\u5f02\u5206\u6790\u7bb1\u7ebf\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">boxplot_cibersort<- ggplot(cibersort, aes(x = celltype, y = composition))+ <\/span><\/code><code><span class=\"code-snippet_outer\"> labs(y=<span class=\"code-snippet__string\">\"Cell composition\"<\/span>,x= <span class=\"code-snippet__string\">\"\"<\/span>)+ <\/span><\/code><code><span class=\"code-snippet_outer\"> geom_boxplot(aes(fill = Group),position=position_dodge(<span class=\"code-snippet__number\">0.5<\/span>),width=<span class=\"code-snippet__number\">0.5<\/span>)+ <\/span><\/code><code><span class=\"code-snippet_outer\"> scale_fill_npg()+<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__meta\">#\u4fee\u6539\u4e3b\u9898<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">theme_bw() + <\/span><\/code><code><span class=\"code-snippet_outer\"> theme(axis.title = element_text(size = <span class=\"code-snippet__number\">12<\/span>,color =<span class=\"code-snippet__string\">\"black\"<\/span>), <\/span><\/code><code><span class=\"code-snippet_outer\"> axis.text = element_text(size= <span class=\"code-snippet__number\">12<\/span>,color = <span class=\"code-snippet__string\">\"black\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> panel.grid.minor.y = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> panel.grid.minor.x = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.text.x = element_text(angle = <span class=\"code-snippet__number\">45<\/span>, hjust = <span class=\"code-snippet__number\">1<\/span> ),<\/span><\/code><code><span class=\"code-snippet_outer\"> panel.grid=element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.position = <span class=\"code-snippet__string\">\"top\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.text = element_text(size= <span class=\"code-snippet__number\">12<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.title= element_text(size= <span class=\"code-snippet__number\">12<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> ) +<\/span><\/code><code><span class=\"code-snippet_outer\"> stat_compare_means(aes(<span class=\"code-snippet__keyword\">group<\/span> = Group),<\/span><\/code><code><span class=\"code-snippet_outer\"> label = <span class=\"code-snippet__string\">\"p.signif\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> method = <span class=\"code-snippet__string\">\"wilcox.test\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> hide.ns = T)<span class=\"code-snippet__meta\">#\u9690\u85cf\u4e0d\u663e\u8457\u7684<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggsave(file=<span class=\"code-snippet__string\">\"cibersort.pdf\"<\/span>,boxplot_cibersort,height=<span class=\"code-snippet__number\">10<\/span>,width=<span class=\"code-snippet__number\">15<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6628664495114006\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_26kI3vxBCOP8UVuAb6KKoRCyDwAyCg-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyBMiaebYV0wRXKtNfxorecdlA8xUHjibok7D66XuaQ2BA6PH26kI3vxBCOP8UVuAb6KKoRCyDwAyCg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"614\" style=\"\" \/><\/p>\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\"><strong>5<\/strong><strong>.\u7ed8\u5236\u514d\u75ab\u7ec6\u80de\u542b\u91cf\u67f1\u72b6\u56fe<\/strong><\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: flex-end;\" mpa-from-tpl=\"t\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"transform: translate3d(5px, 0px, 0px) rotateX(180deg);\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 460px;background-color: rgb(244, 171, 189);\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><em style=\"text-align: center;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;letter-spacing: 0.034em;\"><\/em><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u957f\u5bbd\u6570\u636e\u8f6c\u6362<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort_plot<-melt(cibersort_data,id.vars=\u201dSample\u201d)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4fee\u6539\u5217\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">colnames(cibersort_plot)<-c(<span class=\"code-snippet__string\">\"Sample\"<\/span>,<span class=\"code-snippet__string\">\"\"<\/span>celltype<span class=\"code-snippet__string\">\",\"<\/span>composition<span class=\"code-snippet__string\">\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">#\u7ed8\u5236\u514d\u75ab\u7ec6\u80de\u542b\u91cf\u5806\u53e0\u67f1\u72b6\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\">##\u81ea\u5b9a\u4e49\u989c\u8272\u8bbe\u7f6e<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet_outer\">colour = c(brewer.pal(12, \"<\/span>Paired<span class=\"code-snippet__string\">\"),brewer.pal(8, \"<\/span>Dark2<span class=\"code-snippet__string\">\"),brewer.pal(12, \"<\/span>Set3<span class=\"code-snippet__string\">\"))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggplot(data=cibersort_plot,aes(x=Sample,y=composition,fill=celltype))+<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet_outer\"> geom_bar(position=\"<\/span>stack<span class=\"code-snippet__string\">\",stat=\"<\/span>identity<span class=\"code-snippet__string\">\")+ #\u7ed8\u5236\u67f1\u72b6\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> scale_fill_manual(values = colour)+ #\u6dfb\u52a0\u81ea\u5b9a\u4e49\u989c\u8272<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet_outer\"> labs(x=\"<\/span><span class=\"code-snippet__string\">\",y=\"<\/span><span class=\"code-snippet__string\">\",title=\"<\/span>cell proportion<span class=\"code-snippet__string\">\")+ #\u8bbe\u7f6e\u5750\u6807\u8f74<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> scale_y_continuous(expand=c(0,0))+ #\u8bbe\u7f6e\u4e0ey\u8f74\u7684\u95f4\u8ddd\u4e3a0<\/span><\/code><code><span class=\"code-snippet_outer\"> guides(fill = guide_legend(ncol = 1))+ #\u8bbe\u7f6e\u56fe\u4f8b\u6392\u5217\u6210\u4e00\u5217<\/span><\/code><code><span class=\"code-snippet_outer\"> #\u4fee\u6539\u4e3b\u9898<\/span><\/code><code><span class=\"code-snippet_outer\">theme_bw()+<\/span><\/code><code><span class=\"code-snippet_outer\"> theme(legend.key = element_blank(),legend.title = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> panel.grid=element_blank(), <\/span><\/code><code><span class=\"code-snippet_outer\"> axis.text.x=element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.ticks.x=element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> plot.title=element_text(hjust=0.5))<\/span><\/code><code><span class=\"code-snippet_outer\">#\u4fdd\u5b58\u56fe\u7247<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet_outer\">ggsave(\"<\/span>cibersort_barplot.pdf<span class=\"code-snippet__string\">\",width=8,height=8)<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9859437751004017\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_IDOibQKaZ2icgU7t2ynhn37R8Rzicg-3.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyBMiaebYV0wRXKtNfxorecdia1iaLHibpLREUy0MdZmFiaMlJ3W4sMIDOibQKaZ2icgU7t2ynhn37R8Rzicg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"498\" style=\"\" \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u6700\u7ec8\u5c0f\u679c\u6210\u529f\u7684\u5229\u7528CIBERSORT\u7b97\u6cd5\u8fdb\u884c\u4e86\u514d\u75ab\u6d78\u6da6\u5206\u6790\uff0c\u7ed8\u5236\u4e86\u514d\u75ab\u7ec6\u80de\u4e30\u5ea6\u5806\u53e0\u67f1\u72b6\u56fe\u548c\u5206\u7ec4\u5dee\u5f02\u5206\u6790\u7bb1\u7ebf\u56fe\uff0c\u770b\u8d77\u6765\u56fe\u7247\u6548\u679c\u975e\u5e38\u4e0d\u9519\uff0c\u6b22\u8fce\u5927\u5bb6\u548c\u5c0f\u679c\u4e00\u8d77\u8ba8\u8bba\u5b66\u4e60\u5440\uff01<\/span><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4eca\u5929\u5c0f\u679c\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\uff0c\u4e0b\u671f\u5728\u89c1\u5965\u3002<\/span><\/strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><\/span><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;text-align: left;\">\n<section style=\"font-size: 16px;\">\n<section powered-by=\"xiumi.us\" style=\"margin: 10px;justify-content: flex-start;display: flex;flex-flow: row;\">\n<section style=\"padding-right: 10px;padding-left: 10px;display: inline-block;width: 558px;vertical-align: top;border-style: dashed;border-width: 1px;border-color: rgb(62, 62, 62);align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;width: 536px;border-width: 0px 0px 0px 10px;border-style: solid;border-left-color: rgb(144, 215, 236);border-right-color: rgb(46, 172, 109);box-shadow: rgb(0, 0, 0) 0px 0px 0px;border-bottom-left-radius: 0px;\">\n<section powered-by=\"xiumi.us\" style=\"padding-right: 3px;padding-left: 3px;\">\n<section style=\"margin-top: 0.95em;margin-bottom: -0.65em;display: inline-block;width: 520px;vertical-align: top;\">\n<section style=\"width: 520px;\">\n<section style=\"margin-top: -3px;width: 6px;height: 6px;border-radius: 100%;float: right;background-color: rgb(46, 172, 109);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: -1em;padding: 3px 10px;display: inline-block;vertical-align: top;background-color: rgb(255, 255, 255);color: rgb(0, 154, 68);\">\n<p><strong>\u5f80\u671f\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: justify;\">\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491794&idx=1&sn=2b7a4de95169c79202e44813885a8250&chksm=cf7f7017f808f9015689839fe4057fd418bf7b20eb1dfb5a28cd92e56a41b5605e62bfbc16fb&scene=21#wechat_redirect\" textvalue=\"1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\"><span style=\"font-size: 14px;\">1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4<\/span><\/a><br \/><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" 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