{"id":54005,"date":"2024-06-16T13:33:41","date_gmt":"2024-06-16T05:33:41","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=54005"},"modified":"2024-06-16T13:33:41","modified_gmt":"2024-06-16T05:33:41","slug":"r%e8%af%ad%e8%a8%80%e5%8c%85ggbio%e6%8e%a2%e7%b4%a2%e5%9f%ba%e5%9b%a0%e7%bb%84%e6%95%b0%e6%8d%ae%e7%9a%84%e4%bc%98%e9%9b%85%e4%b9%8b%e9%81%93","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=54005","title":{"rendered":"R\u8bed\u8a00\u5305ggbio\u63a2\u7d22\u57fa\u56e0\u7ec4\u6570\u636e\u7684\u4f18\u96c5\u4e4b\u9053"},"content":{"rendered":"


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title=\"\u751f\u4fe1\u6570\u636e\u5e93\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u6570\u636e\u5e93<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=3138222091851530242#wechat_redirect\" title=\"\u751f\u4fe1\u6709\u5173\u7edf\u8ba1\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u6709\u5173\u7edf\u8ba1<\/a><\/p>\n<\/div>\n<p style=\"margin-left: 0pt;text-indent: 0pt;line-height: normal;font-size: 12pt;font-family: "Times New Roman";font-weight: normal;text-align: justify;\" data-mpa-powered-by=\"yiban.io\"><span style=\"font-family: \u5b8b\u4f53;font-size: 12pt;letter-spacing: 0.034em;text-indent: 0pt;text-align: left;\">\u968f\u7740\u751f\u7269\u5b66\u7814\u7a76\u7684\u4e0d\u65ad\u8fdb\u6b65\uff0c\u57fa\u56e0\u7ec4\u6570\u636e\u7684\u751f\u6210\u4e0e\u83b7\u53d6\u53d8\u5f97\u8d8a\u6765\u8d8a\u666e\u904d\u3002\u7136\u800c\uff0c\u5229\u7528\u5e9e\u5927\u7684\u57fa\u56e0\u7ec4\u6570\u636e\u5e76\u4ece\u4e2d\u83b7\u53d6\u6709\u610f\u4e49\u7684\u4fe1\u606f\u662f\u4e00\u9879\u590d\u6742\u4e14\u5177\u6709\u6311\u6218\u6027\u7684\u4efb\u52a1\u3002\u4e3a\u4e86\u5e2e\u52a9\u751f\u7269\u5b66\u5bb6\u548c\u6570\u636e\u79d1\u5b66\u5bb6\u66f4\u52a0\u8f7b\u677e\u5730\u53ef\u89c6\u5316\u548c\u5206\u6790\u57fa\u56e0\u7ec4\u6570\u636e\uff0cR\u8bed\u8a00\u5f00\u53d1\u4e86\u8bb8\u591a\u5f3a\u5927\u7684\u5305\u3002\u5176\u4e2d\uff0cggbio\uff08Grammar of Graphics for Bioinformatics\uff09\u662f\u4e00\u4e2a\u5907\u53d7\u5173\u6ce8\u7684R\u8bed\u8a00\u5305\uff0c\u5b83\u63d0\u4f9b\u4e86\u4e00\u79cd\u4f18\u96c5\u7684\u65b9\u5f0f\u6765\u53ef\u89c6\u5316\u548c\u89e3\u91ca\u57fa\u56e0\u7ec4\u6570\u636e\u3002<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;\">ggbio<\/span><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;\">\u8bed\u8a00\u5305<\/span><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;\">\u7ed3\u5408\u4e86ggplot2\uff08\u4e00\u79cd\u7528\u4e8e\u7ed8\u5236\u9ad8\u8d28\u91cf\u56fe\u5f62\u7684R\u5305\uff09\u548c\u751f\u7269\u4fe1\u606f\u5b66\u7684\u7279\u5b9a\u77e5\u8bc6\uff0c\u4f7f\u7528\u6237\u80fd\u591f\u8f7b\u677e\u5730\u53ef\u89c6\u5316\u548c\u5206\u6790\u57fa\u56e0\u7ec4\u6570\u636e\u3002\u901a\u8fc7\u63d0\u4f9b\u4e00\u7ec4\u76f4\u89c2\u4e14\u6613\u4e8e\u4f7f\u7528\u7684\u51fd\u6570\u548c\u5de5\u5177\uff0cggbio\u4f7f\u5f97\u5728R\u73af\u5883\u4e2d\u521b\u5efa\u6709\u5438\u5f15\u529b\u7684\u57fa\u56e0\u7ec4\u56fe\u5f62\u6210\u4e3a\u53ef\u80fd\uff0c\u8fd9\u4e9b\u56fe\u5f62\u53ef\u4ee5\u5e2e\u52a9\u7814\u7a76\u8005\u66f4\u597d\u5730\u7406\u89e3\u751f\u7269\u5b66\u4e2d\u7684\u5173\u952e\u8fc7\u7a0b\u548c\u73b0\u8c61\u3002<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.38981481481481484\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-11-09_R\u8bed\u8a00\u5305ggbio\u63a2\u7d22\u57fa\u56e0\u7ec4\u6570\u636e\u7684\u4f18\u96c5\u4e4b\u9053\/1_IWicymEetCc6Enf2BkUE4Yz1Ya5e5w.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyuYsqlJnQUJvnS2iciaDibtfbdV0VmHlriaSjYwjFPLp1BLjokHIWicymEetCc6Enf2BkUE4Yz1Ya5e5w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"font-family: \u5b8b\u4f53;font-size: 15px;letter-spacing: 0.578px;white-space: normal;text-align: center;\" \/><\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;\"><em style=\"letter-spacing: 0.578px;white-space: normal;color: rgb(178, 178, 178);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.476px;text-decoration: underline;\">\u516c\u4f17\u53f7\u540e\u53f0\u56de\u590d\u201c111\u201d\u9886\u53d6\u4ee3\u7801\uff0c\u4ee3\u7801\u7f16\u53f7\uff1a231104<\/span><\/em><\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;\">\u8981\u4f7f\u7528<\/span><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;\">ggbio<\/span><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;\">\u5305\uff0c\u53ef\u4ee5\u5728R\u4e2d\u4f7f\u7528\u4ee5\u4e0b\u547d\u4ee4\u8fdb\u884c\u5b89\u88c5\u548c\u52a0\u8f7d\uff1a<\/span><span style=\"font-family: Calibri;font-size: 12pt;text-indent: 0pt;letter-spacing: 0.034em;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">><\/span> install.packages(<span class=\"code-snippet__string\">\"ggbio\"<\/span>) <span class=\"code-snippet__comment\">#\u5b89\u88c5\u8bed\u8a00\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">><\/span> library(ggbio) <span class=\"code-snippet__comment\">#\u52a0\u8f7d\u8bed\u8a00\u5305<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;\">ggbio\u5177\u6709\u591a\u79cd\u529f\u80fd\uff0c\u4f7f\u5176\u6210\u4e3a\u63a2\u7d22\u548c\u5206\u6790\u57fa\u56e0\u7ec4\u6570\u636e\u7684\u6709\u529b\u5de5\u5177\uff1a<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;mso-list: l1 level1 lfo1;margin-left: 0.0pt;text-indent: 0.0pt;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;\">1.<span style=\"font:7.0pt Times New Roman;\"><\/span><\/span><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;\">\u57fa\u56e0\u7ed3\u6784\u53ef\u89c6\u5316\uff1aggbio\u53ef\u4ee5\u53ef\u89c6\u5316\u57fa\u56e0\u7684\u7ed3\u6784\uff0c\u5305\u62ec\u5916\u663e\u5b50\u3001\u5185\u542b\u5b50\u3001UTR\uff08\u975e\u7ffb\u8bd1\u533a\u57df\uff09\u7b49\u4fe1\u606f\u3002\u8fd9\u6709\u52a9\u4e8e\u7814\u7a76\u4eba\u5458\u5feb\u901f\u4e86\u89e3\u57fa\u56e0\u7684\u7ec4\u6210\u548c\u7ed3\u6784\uff0c\u5e2e\u52a9\u8fdb\u4e00\u6b65\u7684\u529f\u80fd\u6ce8\u91ca\u3002<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family: \u5b8b\u4f53;font-size: 12pt;text-indent: 0pt;letter-spacing: 0.034em;\">\u793a\u4f8b\uff1a<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;\">\u5047\u8bbe\u6211\u4eec\u6709\u4e00\u4e2a\u57fa\u56e0\u540d\u4e3a”GENE1″\uff0c\u5176\u5916\u663e\u5b50(exon)\u548c\u5185\u542b\u5b50(intron)\u7684\u4f4d\u7f6e\u4fe1\u606f\u5206\u522b\u4e3a\uff1a<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;\">\u5916\u663e\u5b501\uff1a\u8d77\u59cb\u4f4d\u7f6e\u4e3a100\uff0c\u7ec8\u6b62\u4f4d\u7f6e\u4e3a300<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;\">\u5185\u542b\u5b501\uff1a\u8d77\u59cb\u4f4d\u7f6e\u4e3a301\uff0c\u7ec8\u6b62\u4f4d\u7f6e\u4e3a500<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;\">\u5916\u663e\u5b502\uff1a\u8d77\u59cb\u4f4d\u7f6e\u4e3a501\uff0c\u7ec8\u6b62\u4f4d\u7f6e\u4e3a700<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;\">\u6211\u4eec\u8fd8\u6709\u4e24\u4e2aUTR\uff085′ UTR\u548c3′ UTR\uff09\u7684\u4fe1\u606f\uff1a<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family: Calibri;font-size: 12pt;text-indent: 0pt;letter-spacing: 0.034em;\">5′ UTR\uff1a\u8d77\u59cb\u4f4d\u7f6e\u4e3a50\uff0c\u7ec8\u6b62\u4f4d\u7f6e\u4e3a99<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;\">3′ UTR\uff1a\u8d77\u59cb\u4f4d\u7f6e\u4e3a701\uff0c\u7ec8\u6b62\u4f4d\u7f6e\u4e3a800<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Install and load required packages<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">> install.packages(<span class=\"code-snippet__string\">\"ggbio\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">> library(ggbio)<\/span><\/code><code><span class=\"code-snippet_outer\">> library(GenomicRanges)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Create gene structure data frame<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">> gene_data <- data.frame(<\/span><\/code><code><span class=\"code-snippet_outer\"> gene = <span class=\"code-snippet__string\">\"GENE1\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__built_in\">type<\/span> = c(<span class=\"code-snippet__string\">\"exon\"<\/span>, <span class=\"code-snippet__string\">\"intron\"<\/span>, <span class=\"code-snippet__string\">\"exon\"<\/span>, <span class=\"code-snippet__string\">\"UTR\"<\/span>, <span class=\"code-snippet__string\">\"UTR\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> start = c(100, 301, 501, 50, 701),<\/span><\/code><code><span class=\"code-snippet_outer\"> end = c(300, 500, 700, 99, 800)<\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Convert data frame to GRanges object<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">> gr <- with(gene_data, GRanges(gene, IRanges(start, end), <span class=\"code-snippet__built_in\">type<\/span>=<span class=\"code-snippet__built_in\">type<\/span>)) <\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Plot gene structure using ggbio<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">> autoplot(gr) +<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_fill_manual(values = c(exon=<span class=\"code-snippet__string\">\"lightblue\"<\/span>, intron=<span class=\"code-snippet__string\">\"lightgreen\"<\/span>, UTR=<span class=\"code-snippet__string\">\"orange\"<\/span>)) +<\/span><\/code><code><span class=\"code-snippet_outer\"> labs(title = <span class=\"code-snippet__string\">\"GENE1 structure\"<\/span>, x = <span class=\"code-snippet__string\">\"Location\"<\/span>, y = <span class=\"code-snippet__string\">\"Gene Name\"<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> theme_minimal() +<\/span><\/code><code><span class=\"code-snippet_outer\"> theme(plot.title = element_text(size=14, face=<span class=\"code-snippet__string\">\"bold\"<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.8464646464646465\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-11-09_R\u8bed\u8a00\u5305ggbio\u63a2\u7d22\u57fa\u56e0\u7ec4\u6570\u636e\u7684\u4f18\u96c5\u4e4b\u9053\/2_QlBDUywJck4owHzlhueHzq73MlKNvg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAz3JeicJYr7pOY6FNYBTEIa6UsXFzA8oz8EUfazsicTACNHktQlBDUywJck4owHzlhueHzq73MlKNvg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"990\" height=\"471.5400085449219\" width=\"557.010009765625\" \/><\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;mso-list: l1 level1 lfo1;margin-left: 0.0pt;text-indent: 0.0pt;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;\">2.<span style=\"font:7.0pt Times New Roman;\"><\/span><\/span><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;\">\u67d3\u8272\u4f53\u53ef\u89c6\u5316\uff1aggbio\u53ef\u4ee5\u7ed8\u5236\u67d3\u8272\u4f53\u7684\u957f\u5ea6\u548c\u5206\u5e03\uff0c\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u8bc6\u522b\u53ef\u80fd\u4e0e\u67d0\u4e9b\u7279\u5b9a\u9057\u4f20\u75be\u75c5\u6216\u8868\u578b\u76f8\u5173\u7684\u67d3\u8272\u4f53\u533a\u57df\u3002<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;\">          <o:p> <\/o:p><\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;\">\u793a\u4f8b\uff1a<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u5b89\u88c5\u548c\u52a0\u8f7dggbio\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">> install.packages(<span class=\"code-snippet__string\">\"ggbio\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">> library(ggbio)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u521b\u5efa\u67d3\u8272\u4f53\u957f\u5ea6\u548c\u5206\u5e03\u6570\u636e\u6846<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">> chromosome_data <- data.frame(<\/span><\/code><code><span class=\"code-snippet_outer\"> chromosome = paste<span class=\"code-snippet__number\">0<\/span>(<span class=\"code-snippet__string\">\"Chr\"<\/span>, <span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">22<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__keyword\">length<\/span> = c(<span class=\"code-snippet__number\">249250621<\/span>, <span class=\"code-snippet__number\">243199373<\/span>, <span class=\"code-snippet__number\">198022430<\/span>, <span class=\"code-snippet__number\">191154276<\/span>, <span class=\"code-snippet__number\">180915260<\/span>, <span class=\"code-snippet__number\">171115067<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__number\">159138663<\/span>, <span class=\"code-snippet__number\">146364022<\/span>, <span class=\"code-snippet__number\">141213431<\/span>, <span class=\"code-snippet__number\">135534747<\/span>, <span class=\"code-snippet__number\">135006516<\/span>, <span class=\"code-snippet__number\">133851895<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__number\">115169878<\/span>, <span class=\"code-snippet__number\">107349540<\/span>, <span class=\"code-snippet__number\">102531392<\/span>, <span class=\"code-snippet__number\">90354753<\/span>, <span class=\"code-snippet__number\">81195210<\/span>, <span class=\"code-snippet__number\">78077248<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__number\">59128983<\/span>, <span class=\"code-snippet__number\">63025520<\/span>, <span class=\"code-snippet__number\">48129895<\/span>, <span class=\"code-snippet__number\">51304566<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">) <\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u7ed8\u5236\u67d3\u8272\u4f53\u957f\u5ea6\u548c\u5206\u5e03\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">> ggplot(chromosome_data, aes(<span class=\"code-snippet__keyword\">x<\/span>=chromosome, <span class=\"code-snippet__keyword\">y<\/span>=<span class=\"code-snippet__keyword\">length<\/span>)) +<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_bar(<span class=\"code-snippet__keyword\">stat<\/span>=<span class=\"code-snippet__string\">\"identity\"<\/span>, fill=<span class=\"code-snippet__string\">\"steelblue\"<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> labs(title=<span class=\"code-snippet__string\">\"DNA Length and Distribution\"<\/span>, <span class=\"code-snippet__keyword\">x<\/span>=<span class=\"code-snippet__string\">\"DNA\"<\/span>, <span class=\"code-snippet__keyword\">y<\/span>=<span class=\"code-snippet__string\">\"Length\"<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> theme_minimal() +<\/span><\/code><code><span class=\"code-snippet_outer\"> theme(axis.text.x = element_text(angle = <span class=\"code-snippet__number\">45<\/span>, hjust = <span class=\"code-snippet__number\">1<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.8464646464646465\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-11-09_R\u8bed\u8a00\u5305ggbio\u63a2\u7d22\u57fa\u56e0\u7ec4\u6570\u636e\u7684\u4f18\u96c5\u4e4b\u9053\/3_utp4nty2oLohmLwpj9oQiaHoRvY7LA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAz3JeicJYr7pOY6FNYBTEIa6W4GRHs3zKbhOtibRAJPLQcOkdIutp4nty2oLohmLwpj9oQiaHoRvY7LA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"990\" height=\"468.469970703125\" width=\"553.4099731445312\" \/><\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;mso-list: l1 level1 lfo1;margin-left: 0.0pt;text-indent: 0.0pt;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;\">3.<span style=\"font:7.0pt Times New Roman;\"><\/span><\/span><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;\">\u57fa\u56e0\u7ec4\u8303\u56f4\u5173\u8054\u56fe\uff1aggbio\u53ef\u4ee5\u751f\u6210\u57fa\u56e0\u7ec4\u8303\u56f4\u7684\u5173\u8054\u56fe\uff0c\u5e2e\u52a9\u63ed\u793a\u57fa\u56e0\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u548c\u57fa\u56e0\u7ec4\u7684\u7a7a\u95f4\u7ed3\u6784\u3002<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;\">          <o:p> <\/o:p><\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;\">\u793a\u4f8b\uff1a<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;\">\u5047\u8bbe\u6709\u4e24\u4e2a\u57fa\u56e0\uff0c\u5206\u522b\u4e3a”GeneA”\u548c”GeneB”\uff0c\u5e76\u4e14\u5b83\u4eec\u4e4b\u95f4\u5b58\u5728\u76f8\u4e92\u4f5c\u7528\u3002\u6211\u4eec\u5c06\u4e3a\u6bcf\u4e2a\u57fa\u56e0\u521b\u5efa\u4e00\u4e2a\u5177\u6709\u968f\u673a\u5750\u6807\u7684GRanges\u5bf9\u8c61\uff0c\u5e76\u5728\u67d3\u8272\u4f53\u4e0a\u7ed8\u5236\u8fd9\u4e24\u4e2a\u57fa\u56e0\u7684\u8303\u56f4\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u5b89\u88c5\u548c\u52a0\u8f7dggbio\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">> install.packages(<span class=\"code-snippet__string\">\"ggbio\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">> library(ggbio)<\/span><\/code><code><span class=\"code-snippet_outer\">> library(GenomicRanges)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u521b\u5efa\u57fa\u56e0\u8303\u56f4\u6570\u636e\u6846<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">> geneA <- GRanges(<span class=\"code-snippet__string\">\"chr1\"<\/span>, IRanges(start=1000, end=2000), gene=<span class=\"code-snippet__string\">\"GeneA\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">> geneB <- GRanges(<span class=\"code-snippet__string\">\"chr1\"<\/span>, IRanges(start=3000, end=4000), gene=<span class=\"code-snippet__string\">\"GeneB\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u521b\u5efa\u57fa\u56e0\u8303\u56f4\u5173\u8054\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">> p <- autoplot(c(geneA, geneB), aes(color=gene)) +<\/span><\/code><code><span class=\"code-snippet_outer\">+ scale_color_manual(values=c(<span class=\"code-snippet__string\">\"blue\"<\/span>, <span class=\"code-snippet__string\">\"red\"<\/span>)) +<\/span><\/code><code><span class=\"code-snippet_outer\">+ labs(title=<span class=\"code-snippet__string\">\"\u57fa\u56e0\u7ec4\u8303\u56f4\u5173\u8054\u56fe\"<\/span>, x=<span class=\"code-snippet__string\">\"\u4f4d\u7f6e\"<\/span>, y=<span class=\"code-snippet__string\">\"\u57fa\u56e0\u540d\u79f0\"<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">+ theme_minimal() +<\/span><\/code><code><span class=\"code-snippet_outer\">+ theme(plot.title = element_text(size=14, face=<span class=\"code-snippet__string\">\"bold\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u6dfb\u52a0\u67d3\u8272\u4f53\u8fb9\u754c\u7ebf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">> p + geom_segment(aes(x=1, xend=1, y=0, yend=5000), color=<span class=\"code-snippet__string\">\"gray\"<\/span>, size=1) +<\/span><\/code><code><span class=\"code-snippet_outer\">+ geom_segment(aes(x=5000, xend=5000, y=0, yend=5000), color=<span class=\"code-snippet__string\">\"gray\"<\/span>, size=1) +<\/span><\/code><code><span class=\"code-snippet_outer\">+ annotate(<span class=\"code-snippet__string\">\"text\"<\/span>, x=2500, y=6000, label=<span class=\"code-snippet__string\">\"Chr1\"<\/span>, size=4, color=<span class=\"code-snippet__string\">\"gray\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">Scale <span class=\"code-snippet__keyword\">for<\/span> y is already present.<\/span><\/code><code><span class=\"code-snippet_outer\">Adding another scale <span class=\"code-snippet__keyword\">for<\/span> y, <span class=\"code-snippet__built_in\">which<\/span> will replace the existing scale.<\/span><\/code><code><span class=\"code-snippet_outer\">Scale <span class=\"code-snippet__keyword\">for<\/span> y is already present.<\/span><\/code><code><span class=\"code-snippet_outer\">Adding another scale <span class=\"code-snippet__keyword\">for<\/span> y, <span class=\"code-snippet__built_in\">which<\/span> will replace the existing scale.<\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;mso-fareast-language:ZH-CN;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.8464646464646465\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-11-09_R\u8bed\u8a00\u5305ggbio\u63a2\u7d22\u57fa\u56e0\u7ec4\u6570\u636e\u7684\u4f18\u96c5\u4e4b\u9053\/4_ZBpY4An94a59sWX2w7FicGsFZhxXBA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAz3JeicJYr7pOY6FNYBTEIa6ta8htY0IxgNfz3WUN1hs167pJZBpY4An94a59sWX2w7FicGsFZhxXBA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"990\" height=\"468.4700012207031\" width=\"553.4099731445312\" \/><\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;mso-list: l1 level1 lfo1;margin-left: 0.0pt;text-indent: 0.0pt;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;\">4.<span style=\"font:7.0pt Times New Roman;\"><\/span><\/span><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;\">DNA\u7532\u57fa\u5316\u6570\u636e\u53ef\u89c6\u5316\uff1aggbio\u53ef\u4ee5\u53ef\u89c6\u5316DNA\u7532\u57fa\u5316\u6570\u636e\uff0c\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u7406\u89e3\u8868\u89c2\u9057\u4f20\u5b66\u5728\u57fa\u56e0\u8c03\u63a7\u548c\u8868\u8fbe\u4e2d\u7684\u4f5c\u7528\u3002<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;\">          <o:p> <\/o:p><\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;\">\u793a\u4f8b\uff1a<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;\">\u5047\u8bbe\u6709\u4e00\u4e2a\u5305\u542bDNA\u7532\u57fa\u5316\u6570\u636e\u7684\u6570\u636e\u6846\uff0c\u6bcf\u884c\u4ee3\u8868\u4e00\u4e2a\u7532\u57fa\u5316\u4f4d\u70b9\uff0c\u5e76\u5305\u542b\u7532\u57fa\u5316\u4f4d\u70b9\u7684\u67d3\u8272\u4f53\u3001\u4f4d\u7f6e\u548c\u7532\u57fa\u5316\u6c34\u5e73\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"markdown\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__section\"># \u5b89\u88c5\u548c\u52a0\u8f7dggbio\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__quote\">> install.packages(\"ggbio\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__quote\">> library(ggbio)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__quote\">> library(Gviz)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__code\"> <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__section\"># \u521b\u5efaDNA\u7532\u57fa\u5316\u6570\u636e\u6846<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__quote\">> methylation_data <- data.frame(<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__bullet\">+ <\/span>chromosome = c(\"chr1\", \"chr1\", \"chr1\", \"chr1\", \"chr1\"),<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__bullet\">+ <\/span>position = c(1000, 2000, 3000, 4000, 5000),<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__bullet\">+ <\/span>methylation_level = c(0.8, 0.6, 0.4, 0.2, 0.9)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__bullet\">+ <\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__code\"> <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__section\"># \u521b\u5efa\u57fa\u56e0\u7ec4\u8f68\u9053\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__quote\">> genometrack <- GenomeAxisTrack() +<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__bullet\">+ <\/span>GenomeTickTrack()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__code\"> <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__section\"># \u521b\u5efaDNA\u7532\u57fa\u5316\u6570\u636e\u8f68\u9053\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__quote\">> methylationtrack <- DataTrack(<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__bullet\">+ <\/span>range = GRanges(methylation<span class=\"code-snippet__emphasis\">_data$chromosome, IRanges(methylation_<\/span>data$position, <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__bullet\">+ <\/span>width=1)),<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__bullet\">+ <\/span>data = methylation<span class=\"code-snippet__emphasis\">_data$methylation_<\/span>level,<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__bullet\">+ <\/span>type = \"l\", ylim = c(0, 1),<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__bullet\">+ <\/span>name = \"DNA Methylation\",<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__bullet\">+ <\/span>lty = 1, col = \"blue\"<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__bullet\">+ <\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__code\"> <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__section\"># \u7ed8\u5236DNA\u7532\u57fa\u5316\u6570\u636e\u53ef\u89c6\u5316\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__quote\">> plotTracks(genometrack, methylationtrack, type=\"p\", chromosome=\"chr1\")<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><o:page><\/o:page><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;mso-fareast-language:ZH-CN;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"1\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-11-09_R\u8bed\u8a00\u5305ggbio\u63a2\u7d22\u57fa\u56e0\u7ec4\u6570\u636e\u7684\u4f18\u96c5\u4e4b\u9053\/5_icUpTHxl1AIyfo6lLuCk86MSZfsFuw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAz3JeicJYr7pOY6FNYBTEIa6XyRC0zuuNiaFUvkDwcp5TlY6U0icUpTHxl1AIyfo6lLuCk86MSZfsFuw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1000\" height=\"553.010009765625\" width=\"553.010009765625\" \/><\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;mso-list: l1 level1 lfo1;margin-left: 0.0pt;text-indent: 0.0pt;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;\">5.<span style=\"font:7.0pt Times New Roman;\"><\/span><\/span><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;\">\u67d3\u8272\u8d28\u72b6\u6001\u56fe\uff1aggbio\u652f\u6301\u7ed8\u5236\u67d3\u8272\u8d28\u72b6\u6001\u56fe\uff0c\u8fd9\u6709\u52a9\u4e8e\u7814\u7a76\u4eba\u5458\u4e86\u89e3\u57fa\u56e0\u7ec4\u5728\u4e0d\u540c\u751f\u7269\u5b66\u72b6\u6001\u4e0b\u7684\u7a7a\u95f4\u7ed3\u6784\u3002<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;\">\u793a\u4f8b\uff1a<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;font-variant:normal;text-transform:none;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;\">\u67d3\u8272\u8d28\u72b6\u6001\u6570\u636e\u53ef\u4ee5\u901a\u8fc7\u4e0d\u540c\u7684\u5b9e\u9a8c\u6280\u672f\uff08\u5982ChIP-seq\u6216ATAC-seq\uff09\u6765\u83b7\u5f97\uff0c\u7528\u4e8e\u63cf\u8ff0\u57fa\u56e0\u7ec4\u4e0a\u4e0d\u540c\u533a\u57df\u7684\u67d3\u8272\u8d28\u72b6\u6001\u3002\u5728\u8fd9\u91cc\uff0c\u6211\u4eec\u4f7f\u7528\u968f\u673a\u751f\u6210\u7684\u6570\u636e\u6765\u521b\u5efa\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u5b89\u88c5\u548c\u52a0\u8f7dggbio\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">> install.packages(<span class=\"code-snippet__string\">\"ggbio\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">> library(ggbio)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u521b\u5efa\u67d3\u8272\u8d28\u72b6\u6001\u6570\u636e\u6846\uff08\u793a\u4f8b\u4f7f\u7528\u968f\u673a\u751f\u6210\u7684\u6570\u636e\uff09<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">> chromatin_data <- data.frame(<\/span><\/code><code><span class=\"code-snippet_outer\"> chromosome = rep(paste<span class=\"code-snippet__number\">0<\/span>(<span class=\"code-snippet__string\">\"chr\"<\/span>, <span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">22<\/span>), <span class=\"code-snippet__keyword\">each<\/span>=<span class=\"code-snippet__number\">100<\/span>), <span class=\"code-snippet__comment\"># \u5047\u8bbe\u670922\u6761\u67d3\u8272\u4f53\uff0c\u6bcf\u6761\u67d3\u8272\u4f53\u6709100\u4e2a\u533a\u57df<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> start = rep(se<span class=\"code-snippet__string\">q(1, 1000000, by=10000)<\/span>, <span class=\"code-snippet__keyword\">times<\/span>=<span class=\"code-snippet__number\">22<\/span>), <span class=\"code-snippet__comment\"># \u6bcf\u4e2a\u533a\u57df\u8d77\u59cb\u4f4d\u7f6e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> end = rep(se<span class=\"code-snippet__string\">q(10000, 1000010, by=10000)<\/span>, <span class=\"code-snippet__keyword\">times<\/span>=<span class=\"code-snippet__number\">22<\/span>), <span class=\"code-snippet__comment\"># \u6bcf\u4e2a\u533a\u57df\u7ec8\u6b62\u4f4d\u7f6e <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__keyword\">state<\/span> = sample(c(<span class=\"code-snippet__string\">\"Active\"<\/span>, <span class=\"code-snippet__string\">\"Inactive\"<\/span>, <span class=\"code-snippet__string\">\"Enhancer\"<\/span>, <span class=\"code-snippet__string\">\"Promoter\"<\/span>), <span class=\"code-snippet__number\">2200<\/span>, replace=TRUE) <span class=\"code-snippet__comment\"># \u968f\u673a\u751f\u6210\u67d3\u8272\u8d28\u72b6\u6001<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u5c06\u72b6\u6001\u4fe1\u606f\u6620\u5c04\u4e3a\u989c\u8272<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">> chromatin_data$color <- ifelse(chromatin_data$state == <span class=\"code-snippet__string\">\"Active\"<\/span>, <span class=\"code-snippet__string\">\"red\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> ifelse(chromatin_data$state == <span class=\"code-snippet__string\">\"Inactive\"<\/span>, <span class=\"code-snippet__string\">\"blue\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> ifelse(chromatin_data$state == <span class=\"code-snippet__string\">\"Enhancer\"<\/span>, <span class=\"code-snippet__string\">\"green\"<\/span>, <span class=\"code-snippet__string\">\"purple\"<\/span>)))<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u521b\u5efa\u57fa\u56e0\u7ec4\u8f68\u9053\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">> genometrack <- GenomeAxisTrack() +<\/span><\/code><code><span class=\"code-snippet_outer\"> GenomeTickTrack()<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u521b\u5efa\u67d3\u8272\u8d28\u72b6\u6001\u8f68\u9053\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">> chromatin_track <- DataTrack(<\/span><\/code><code><span class=\"code-snippet_outer\"> range = GRanges(chromatin_data$chromosome, IRanges(chromatin_data$start, chromatin_data$end)),<\/span><\/code><code><span class=\"code-snippet_outer\"> data = chromatin_data$color,<\/span><\/code><code><span class=\"code-snippet_outer\"> type = <span class=\"code-snippet__string\">\"histogram\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> alpha = <span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">8<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u7ed8\u5236\u67d3\u8272\u8d28\u72b6\u6001\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">> ggplot() +<\/span><\/code><code><span class=\"code-snippet_outer\"> genometrack +<\/span><\/code><code><span class=\"code-snippet_outer\"> chromatin_track +<\/span><\/code><code><span class=\"code-snippet_outer\"> theme_ggbio()<\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;mso-fareast-language:ZH-CN;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.9212962962962963\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-11-09_R\u8bed\u8a00\u5305ggbio\u63a2\u7d22\u57fa\u56e0\u7ec4\u6570\u636e\u7684\u4f18\u96c5\u4e4b\u9053\/6_kL9NYWsMqHy5y5W5YFNt03fp7EYzRA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAz3JeicJYr7pOY6FNYBTEIa6EHA5WzkM3lcOOxxoZrhEUzLhkL9NYWsMqHy5y5W5YFNt03fp7EYzRA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"462.4100341796875\" width=\"502.07000732421875\" \/><\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;\">ggbio\u4e4b\u6240\u4ee5\u5907\u53d7\u6b22\u8fce\uff0c\u662f\u56e0\u4e3a\u5b83\u878d\u5408\u4e86ggplot2\u7684\u4f18\u96c5\u548c\u7075\u6d3b\u6027\uff0c\u4f7f\u7528\u6237\u80fd\u591f\u901a\u8fc7\u7b80\u5355\u7684\u4ee3\u7801\u521b\u5efa\u590d\u6742\u7684\u56fe\u5f62\u3002\u540c\u65f6\uff0cggbio\u4e13\u95e8\u9488\u5bf9\u751f\u7269\u4fe1\u606f\u5b66\u6570\u636e\u8fdb\u884c\u4e86\u4f18\u5316\uff0c\u4f7f\u5f97\u7814\u7a76\u4eba\u5458\u80fd\u591f\u4e13\u6ce8\u4e8e\u6570\u636e\u672c\u8eab\uff0c\u800c\u4e0d\u5fc5\u8fc7\u591a\u8003\u8651\u56fe\u5f62\u7ec6\u8282\u3002<\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;text-indent: 0pt;line-height: normal;font-size: 12pt;font-family: "Times New Roman";font-weight: normal;\"><span style=\"font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;font-variant:normal;text-transform:none;\">\u4ee5\u4e0a\u5c31\u662f\u5bf9R\u8bed\u8a00\u5305ggbio\u7684\u7b80\u5355\u4ecb\u7ecd\u5566\uff0c\u5b83\u4e3a\u7814\u7a76\u4eba\u5458\u63d0\u4f9b\u4e86\u4e00\u79cd\u4f18\u96c5\u7684\u65b9\u5f0f\u6765\u53ef\u89c6\u5316\u57fa\u56e0\u7ec4\u6570\u636e\uff0c\u5e2e\u52a9\u4ed6\u4eec\u66f4\u6df1\u5165\u5730\u7406\u89e3\u751f\u7269\u5b66\u4e2d\u7684\u91cd\u8981\u8fc7\u7a0b\u3002\u4f5c\u4e3aR\u8bed\u8a00\u751f\u6001\u7cfb\u7edf\u4e2d\u7684\u91cd\u8981\u6210\u5458\uff0cggbio\u4e3a\u751f\u7269\u5b66\u5bb6\u548c\u6570\u636e\u79d1\u5b66\u5bb6\u4eec\u63d0\u4f9b\u4e86\u4e00\u4e2a\u5f3a\u5927\u7684\u5de5\u5177\uff0c\u4fc3\u8fdb\u4e86\u57fa\u56e0\u7ec4\u5b66\u548c\u751f\u7269\u4fe1\u606f\u5b66\u9886\u57df\u7684\u7814\u7a76\u548c\u53d1\u5c55\u3002<\/span><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: 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