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style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">2.\u6839\u636e\u53c2\u8003\u57fa\u56e0\u96c6\u7684\u547d\u540d\u6ce8\u91ca\u805a\u5408\u6bcf\u4e2a\u7ec6\u80de\u7c7b\u578b\u7684\u591a\u91cd\u76f8\u5173\u7cfb\u6570\uff0c\u6bcf\u79cd\u7ec6\u80de\u7c7b\u578b\u7684\u8bc4\u5206\u9ed8\u8ba4\u9009\u62e9\u76f8\u5173\u7cfb\u6570\u503c\u768480%\uff0c\u6b64\u65f6\u53ef\u4ee5\u7b5b\u9009\u5f97\u5230\u6bcf\u4e2a\u7ec6\u80de\u6700\u53ef\u80fd\u7684\u7ec6\u80de\u5b9a\u4e49\u7c7b\u578b\uff1b<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">3.\u8ba1\u7b97\u6765\u81ea\u53c2\u8003\u6570\u636e\u96c6\u4e2d\u6700\u53ef\u80fd\u7684\u7ec6\u80de\u7c7b\u578b\u57fa\u56e0\u8868\u8fbe\u8c31\u7684\u53ef\u53d8\u57fa\u56e0\uff0c\u5e76\u8ba1\u7b97\u5355\u4e2a\u7ec6\u80de\u4e0e\u6700\u53ef\u80fd\u7ec6\u80de\u7c7b\u578b\u5728\u53ef\u53d8\u57fa\u56e0\u4e0a\u7684Spearman\u76f8\u5173\u6027\uff0c\u53bb\u9664\u6700\u4f4e\u503c\u7684\u7ec6\u80de\uff08\u6216\u662f\u6bd4\u6700\u9ad8\u503c\u4f4e\u4e8e0.05\u7684\u7ec6\u80de\uff09\uff0c\u91cd\u590d\u6b64\u6b65\u9aa4\uff0c\u76f4\u5230\u53ea\u4fdd\u7559\u4e24\u79cd\u7ec6\u80de\u7c7b\u578b\u3002\u6700\u540e\u4e00\u6b21\u8fd0\u884c\u540e\uff0c\u76f8\u5173\u7cfb\u6570\u503c\u6700\u9ad8\u7684\u7ec6\u80de\u7c7b\u578b\u5219\u4f5c\u4e3a\u9700\u8981\u9274\u5b9a\u7684\u7ec6\u80de\u7684\u5b9a\u4e49\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e00\u3001\u51c6\u5907<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><span style=\"color: rgb(16, 116, 231);\"><strong>\uff08\u4e00\uff09\u8f6f\u4ef6\u5b89\u88c5\u4e0e\u52a0\u8f7d<\/strong><\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">BiocManager::install(<span class=\"code-snippet__string\">\"SingleR\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">library(SingleR)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong><span style=\"color: rgb(16, 116, 231);\">\uff08\u4e8c\uff09\u53c2\u8003\u57fa\u56e0\u5e93<\/span><\/strong><br \/><\/p>\n<p>\u4ed6\u73b0\u5728\u6709\u4e03\u4e2a\u5e93\uff0c\u4e3b\u8981\u6709\u4eba\u548c\u5c0f\u9f20\u7684<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3094660194174757\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/3_uWnJL8wCNuKzgiacoFu5CqHiblMCXw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4ATr9dMvztGBu5na4JshPicPRQia1uWnJL8wCNuKzgiacoFu5CqHiblMCXw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"824\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.2674698795180723\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/4_tg1KlrdJunzGJ2D6QzDa7C5kc8Aeug.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4NIgoL675gbpcE9OYAoqjlW4ttg1KlrdJunzGJ2D6QzDa7C5kc8Aeug\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"830\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4e0b\u8f7d\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(celldex)<\/span><\/code><code><span class=\"code-snippet_outer\">ref <- celldex::HumanPrimaryCellAtlasData() <span class=\"code-snippet__comment\">#\u4e0b\u8f7d\u4eba\u7c7b\u7ec6\u80de\u6570\u636e<\/span><\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u4eca\u5929\u4f7f\u7528\u7684\u6848\u4f8b\u662f\u7528\u5916\u5468\u8840\u7ec6\u80de\u53ef\u4ee5\u7528Monaco Inunene Data\u3002\u76ee\u524d singler \u5df2\u7ecf\u5c06\u8fd9\u4e9b\u6570\u636e\u5e93\u6253\u5305\u5230 celldex \u5305\u4e2d\uff0c\u53ef\u4ee5\u901a\u8fc7\u5b89\u88c5 celldex \u5305\u8fdb\u884c\u5b89\u88c5\u548c\u4e0b\u8f7d\u3002\u4e0b\u8f7d\u7684\u6570\u636e\u4e3a SummarizedExperiment \u7c7b\u5bf9\u8c61\uff0c\u53ef\u4ee5\u901a\u8fc7\u6dfb\u52a0 Ensemble=TRUE\uff0c\u4e0b\u8f7d Ensemble \u7c7b\u578b\u57fa\u56e0 ID\uff0c\u901a\u8fc7\u6dfb\u52a0 cell.ont = c(“all”, “nonna”, “none”) \u6dfb\u52a0 Cell Ontology terms \u4fe1\u606f\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3924170616113744\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/5_p3cib8FicDCXPKdD8oeUwEF17npBQA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4O6DzIBWnv6oPHLjBUVyBico1JibMp3cib8FicDCXPKdD8oeUwEF17npBQA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1055\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"color: rgb(16, 116, 231);padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\uff08\u4e09\uff09\u53c2\u8003\u57fa\u56e0\u521d\u63a2<\/strong><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.45695364238410596\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/6_NicFPap7yvrDYjm2tBamzDBsyFd25Q.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4wkZYUZg5QLyiaj9Aib8Qms8fdUuNicFPap7yvrDYjm2tBamzDBsyFd25Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"755\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u5217\u662f\u6bcf\u4e2a\u7ec6\u80de\uff0c\u5171713\u79cd\u3002<\/p>\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e8c\u3001SingleR\u6ce8\u91ca<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><span style=\"color: rgb(16, 116, 231);\"><strong>\uff08\u4e00\uff09\u8bfb\u53d6\u6570\u636e<\/strong><\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6seurat\u5305\u5206\u6790\u7684\u5355\u7ec6\u80de\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc.data <- readRDS(\u201cxxx.rds<span class=\"code-snippet__string\">\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">#\u83b7\u5f97\u5355\u7ec6\u80de\u8868\u8fbefeature_barcode\u77e9\u9635<\/span><\/code><code><span class=\"code-snippet_outer\">query <- pbmc@assays$RNA@data<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.42276422764227645\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/7_ROY61ajLnJWBw7nVYZfEK5eojo4vUw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4YWzVfFW8xLB6Q8ibCfwkMAjwnROY61ajLnJWBw7nVYZfEK5eojo4vUw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"246\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u901a\u8fc7\u67e5\u770b\u53ef\u4ee5\u53d1\u73b0\u662f\u77e9\u9635\u683c\u5f0f\u3002<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.28393881453154873\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/8_rAyA5Xansib31W2qlP0ZlE0LtZDWqw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4bTNiaQSFASHwFV5RaR2HXW6eYGrAyA5Xansib31W2qlP0ZlE0LtZDWqw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1046\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u884c\u540d\u662f\u57fa\u56e0\uff0c\u5217\u662f\u5355\u7ec6\u80de\u7684barcode\u7684\u4e00\u4e2a\u7a00\u758f\u77e9\u9635\u3002<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><span style=\"color: rgb(16, 116, 231);\"><strong>\uff08\u4e8c\uff09\u6ce8\u91ca\u7ec6\u80de\u540d<\/strong><\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#SingleR\u6ce8\u91ca<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pred.pbmc<-SingleR(test=query,assay.type.test=<span class=\"code-snippet__number\">1<\/span>,ref=ref,clusters=pbmc@active.ident,labels=ref$label.fine)<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;border-width: 1px;border-radius: 8px;border-style: dotted;border-color: rgb(0, 0, 0);overflow: hidden;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin: 15px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);padding-right: 15px;padding-left: 15px;\">\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">Test \u8f93\u5165\u8868\u8fbe\u77e9\u9635<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">Ref \u4e0b\u8f7d\u597d\u7684\u53c2\u8003\u6570\u636e<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">Cluster \u7ec6\u80de\u5206\u7fa4\u6570<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">Lab \u4f7f\u7528\u6570\u636e\u81ea\u5e26\u7684\u7ec6\u80de\u540d<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.4305555555555556\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/9_2uSsDOAibw5ojcV8KeibmbDnnF8ZVw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4GXwueQDSM7cs6jUH6TIpWfGs2b2uSsDOAibw5ojcV8KeibmbDnnF8ZVw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u5177\u4f53\u8be6\u7ec6\u7684\u53c2\u6570\u4fe1\u606f\uff0c\u53ef\u4ee5\u8f93\u5165\uff1fSingleR\u67e5\u770b\u5e2e\u52a9\u6587\u6863<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.19722222222222222\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/10_icrtOpMZSI3Jklric8icv7gAj4B7dg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4nLQvGL01HPcHS8GQ9P6uwSmCpGCicrtOpMZSI3Jklric8icv7gAj4B7dg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u6ce8\u91ca\u597d\u7684\u6570\u636e<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u67e5\u770b\u6ce8\u91ca\u7ed3\u679c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">View(as.data.frame(pred.pbmc))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8f93\u51fa\u6ce8\u91ca\u7684\u7ec6\u80de<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pred.pbmc$pruned.labels<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.08981481481481482\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/11_jibxqJgibPeiamib1ltz1pJv0qfmCQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4F639mGF3FVWjw2vTEREn9yR1riaIRjibxqJgibPeiamib1ltz1pJv0qfmCQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ec6\u80de\u7c7b\u522b\u7edf\u8ba1<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">table(pred.pbmc$pruned.labels)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.20462962962962963\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/12_XSrH63hCZvNOpjnAUtogSy1PCKzvgg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4NQJRNtKiaxhQoa5IIeI51O6p9XSrH63hCZvNOpjnAUtogSy1PCKzvgg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u5171\u8ba17\u4e2a\u7ec6\u80de\u7c7b<\/p>\n<p><br \/><\/p>\n<p><span style=\"color: rgb(16, 116, 231);\"><strong>\uff08\u4e09\uff09\u4f7f\u7528\u6ce8\u91ca\u7684\u7ec6\u80de\u540d\u79f0<\/strong><\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u521b\u5efa\u4e00\u4e2a\u65b0\u7684\u53d8\u91cf\u50a8\u5b58\u7ec6\u80de\u540d\u79f0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">new.cluster.ids <- pred.pbmc$labels<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.15568022440392706\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/13_qeM4sQUGjpov8NbXhVysse1uPibuBw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4TzQGiaXf2LN0fOdhSLly6OEzUdqeM4sQUGjpov8NbXhVysse1uPibuBw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"713\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>9\u79cd\u7ec6\u80de\u540d\u79f0<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u66ff\u6362level\u4e3a\u7ec6\u80de\u540d\uff0c\u5c06level\u4ece\u6570\u5b57\u66ff\u6362\u6210\u7ec6\u80de\u540d\u79f0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">names<\/span>(new.cluster.ids) <- levels(pbmc)<\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- RenameIdents(pbmc,new.cluster.ids)<\/span><\/code><code><span class=\"code-snippet_outer\">levels(pbmc)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.19536903039073805\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/14_ty3Y7q7BibcbJ98AKSnP3cSTdtoLbA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx47K6yMEaISrN2rbvRLOWddqKXUty3Y7q7BibcbJ98AKSnP3cSTdtoLbA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"691\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong style=\"color: rgb(16, 116, 231);\">\uff08\u56db\uff09\u7ed8\u56fe<\/strong><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u67e5\u770b\u6ce8\u91ca\u7ed3\u679c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">plotScoreHeatmap(pred.pbmc)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7194444444444444\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/15_yXoTdp6yZcQ41c9v29XfJiaxp0Z0zw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4B1kC13jjqZ8KwIMXqDD2n960byXoTdp6yZcQ41c9v29XfJiaxp0Z0zw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u7531\u7d2b\u5230\u9ec4\uff0c\u76f8\u5173\u6027\u4ece\u4f4e\u5230\u9ad8\u3002\u5206\u6210\u4e86\u5176\u4e2d\u7ec6\u80de\u3002<\/p>\n<p><br \/><\/p>\n<p><br \/><\/p>\n<p><span style=\"color: rgb(16, 116, 231);\"><strong>\uff08\u4e94\uff09\u4e3a\u7ec6\u80de\u7fa4\u6dfb\u52a0\u6ce8\u91ca<\/strong><\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236umap\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">DimPlot(pbmc, reduction = <span class=\"code-snippet__string\">\"umap\"<\/span>, label = <span class=\"code-snippet__literal\">TRUE<\/span>, pt.size = <span class=\"code-snippet__number\">0.5<\/span>) + NoLegend()<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9976608187134502\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/16_ia2zfVsT8csYlfwP72P3XYaNU1Kvjw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4Iyk0SvKGqrcQMyA5s8d3ia7ws6ia2zfVsT8csYlfwP72P3XYaNU1Kvjw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"855\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236t-SNE\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">DimPlot(pbmc, reduction = <span class=\"code-snippet__string\">\"tsne\"<\/span>, label = <span class=\"code-snippet__literal\">TRUE<\/span>, pt.size = <span class=\"code-snippet__number\">0.5<\/span>) + NoLegend()<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9849012775842044\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/17_bXqsbOQMj9JANlRHZhtaUyysMItdCw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4d0gnyhTBDBZXNINrXPR6PZdwibXqsbOQMj9JANlRHZhtaUyysMItdCw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"861\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 98%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;border-style: solid;border-width: 2px;border-color: rgb(160, 160, 160);padding: 27px;\">\n<section style=\"text-align: justify;line-height: 1.6;letter-spacing: 0px;padding-right: 6px;padding-left: 6px;\" powered-by=\"xiumi.us\">\n<p>\u53ef\u4ee5\u770b\u5230\u6210\u529f\u5c06\u7ec6\u80de\u7c7b\u578b\u6ce8\u91ca\u5230\u7ec6\u80de\u7fa4\u4e0a\u4e86\u3002\u4f60\u5b66\u4f1a\u4e86\u5417\uff01\u4e0b\u671f\u5c0f\u82b1\u5c06\u4e3a\u5927\u5bb6\u5e26\u6765\u57fa\u4e8eOrthoFinder\u7ed3\u679c\u5f00\u5c55\u7684\u7cfb\u7edf\u53d1\u80b2\u57fa\u56e0\u7ec4\u5b66\u5206\u6790\u7684\u6559\u7a0b\uff0c\u611f\u5174\u8da3\u7684\u53ef\u4ee5\u6301\u7eed\u5173\u6ce8\u672c\u516c\u4f17\u53f7\u54e6\uff01<\/p>\n<p><br \/><\/p>\n<p>\u6b22\u8fce\u4f7f\u7528\uff1a\u4e91\u751f\u4fe1 – \u5b66\u751f\u7269\u4fe1\u606f\u5b66 (biocloudservice.com)<\/p>\n<p><br \/><\/p>\n<p>\u5982\u679c\u60f3\u7528\u670d\u52a1\u5668\u53ef\u4ee5\u8054\u7cfb\u5fae\u4fe1\uff1a18502195490\uff08\u5feb\u6765\u8054\u7cfb\u6211\u4eec\u4f7f\u7528\u5427\uff01\uff09<\/p>\n<\/section>\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row;transform: translate3d(40px, 0px, 0px);margin-bottom: -40px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 26px;height: 26px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);border-left: 1px solid rgb(160, 160, 160);border-bottom-left-radius: 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650761303&idx=2&sn=6f73471d1e82c56c0b2160d0fcd32d53&scene=21#wechat_redirect\" textvalue=\"\" linktype=\"image\" imgurl=\"https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/0?wx_fmt=jpeg\" imgdata=\"[object Object]\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.35083798882681566\" data-s=\"300,640\" 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5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;margin-right: -17px;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(201, 229, 70);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u4eca\u5929\u5c0f\u82b1\u4e3a\u5927\u5bb6\u5206\u4eab\u4e00\u4e2a\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\u7684\u4e00\u4e2a\u6743\u5a01R\u5305SingleR\u3002<br \/><\/p>\n<p><br \/><\/p>\n<p>\u5176\u4e4b\u524d\u53d1\u8fc7nature\uff0c\u6536\u5230\u4e86\u975e\u5e38\u591a\u4eba\u7684\u8ba4\u53ef\uff0c\u662f\u9274\u5b9a\u4e0e\u6ce8\u91ca\u5355\u7ec6\u80de\u4e9a\u7fa4\u7684number one\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5203703703703704\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/1_3ia2MYiavsAXSDibvKibGPF9rT9kzg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4by1XpcibV7X28pXHhhC7yplA3rfQe3ia2MYiavsAXSDibvKibGPF9rT9kzg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u622a\u81f3\u76ee\u524d\u996e\u7528\u91cf\u5df2\u8fbe1286\u6b21\u3002<\/p>\n<p>SingleR\u7684\u4f7f\u7528\u6587\u6863\uff0c\u975e\u5e38\u8be6\u7ec6\u3002<\/p>\n<p>\u4f7f\u7528 SingleR \u5206\u914d\u5355\u5143\u683c\u7c7b\u578b (bioconductor.org)<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5509259259259259\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/2_wKu0J6mcLA14gn2Uk5vQvLajHmu4aw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4Dfvib1hQxmpQ0QIfd8Bl0pkR6wKu0J6mcLA14gn2Uk5vQvLajHmu4aw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u539f\u7406\u793a\u610f\u56fe<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u539f\u7406<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;border-width: 1px;border-radius: 8px;border-style: dotted;border-color: rgb(0, 0, 0);overflow: hidden;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin: 15px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);padding-right: 15px;padding-left: 15px;\">\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">1.\u8ba1\u7b97\u53c2\u8003\u6570\u636e\u96c6\u4e2d\u5404\u4e2a\u7ec6\u80de\u7c7b\u578b\u57fa\u56e0\u8868\u8fbe\u8c31\u7684\u53ef\u53d8\u57fa\u56e0\uff0c\u5bf9\u4e8e\u6bcf\u4e00\u4e2a\u9700\u8981\u9274\u5b9a\u7684\u7ec6\u80de\uff0c\u8ba1\u7b97\u5355\u4e2a\u7ec6\u80de\u4e0e\u53c2\u8003\u6570\u636e\u96c6\u4e2d\u5404\u4e2a\u7ec6\u80de\u7c7b\u578b\u57fa\u56e0\u5728\u53ef\u53d8\u57fa\u56e0\u4e0a\u7684Spearman\u76f8\u5173\u6027\uff1b<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">2.\u6839\u636e\u53c2\u8003\u57fa\u56e0\u96c6\u7684\u547d\u540d\u6ce8\u91ca\u805a\u5408\u6bcf\u4e2a\u7ec6\u80de\u7c7b\u578b\u7684\u591a\u91cd\u76f8\u5173\u7cfb\u6570\uff0c\u6bcf\u79cd\u7ec6\u80de\u7c7b\u578b\u7684\u8bc4\u5206\u9ed8\u8ba4\u9009\u62e9\u76f8\u5173\u7cfb\u6570\u503c\u768480%\uff0c\u6b64\u65f6\u53ef\u4ee5\u7b5b\u9009\u5f97\u5230\u6bcf\u4e2a\u7ec6\u80de\u6700\u53ef\u80fd\u7684\u7ec6\u80de\u5b9a\u4e49\u7c7b\u578b\uff1b<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">3.\u8ba1\u7b97\u6765\u81ea\u53c2\u8003\u6570\u636e\u96c6\u4e2d\u6700\u53ef\u80fd\u7684\u7ec6\u80de\u7c7b\u578b\u57fa\u56e0\u8868\u8fbe\u8c31\u7684\u53ef\u53d8\u57fa\u56e0\uff0c\u5e76\u8ba1\u7b97\u5355\u4e2a\u7ec6\u80de\u4e0e\u6700\u53ef\u80fd\u7ec6\u80de\u7c7b\u578b\u5728\u53ef\u53d8\u57fa\u56e0\u4e0a\u7684Spearman\u76f8\u5173\u6027\uff0c\u53bb\u9664\u6700\u4f4e\u503c\u7684\u7ec6\u80de\uff08\u6216\u662f\u6bd4\u6700\u9ad8\u503c\u4f4e\u4e8e0.05\u7684\u7ec6\u80de\uff09\uff0c\u91cd\u590d\u6b64\u6b65\u9aa4\uff0c\u76f4\u5230\u53ea\u4fdd\u7559\u4e24\u79cd\u7ec6\u80de\u7c7b\u578b\u3002\u6700\u540e\u4e00\u6b21\u8fd0\u884c\u540e\uff0c\u76f8\u5173\u7cfb\u6570\u503c\u6700\u9ad8\u7684\u7ec6\u80de\u7c7b\u578b\u5219\u4f5c\u4e3a\u9700\u8981\u9274\u5b9a\u7684\u7ec6\u80de\u7684\u5b9a\u4e49\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e00\u3001\u51c6\u5907<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><span style=\"color: rgb(16, 116, 231);\"><strong>\uff08\u4e00\uff09\u8f6f\u4ef6\u5b89\u88c5\u4e0e\u52a0\u8f7d<\/strong><\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">BiocManager::install(<span class=\"code-snippet__string\">\"SingleR\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">library(SingleR)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong><span style=\"color: rgb(16, 116, 231);\">\uff08\u4e8c\uff09\u53c2\u8003\u57fa\u56e0\u5e93<\/span><\/strong><br \/><\/p>\n<p>\u4ed6\u73b0\u5728\u6709\u4e03\u4e2a\u5e93\uff0c\u4e3b\u8981\u6709\u4eba\u548c\u5c0f\u9f20\u7684<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3094660194174757\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/3_uWnJL8wCNuKzgiacoFu5CqHiblMCXw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4ATr9dMvztGBu5na4JshPicPRQia1uWnJL8wCNuKzgiacoFu5CqHiblMCXw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"824\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.2674698795180723\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/4_tg1KlrdJunzGJ2D6QzDa7C5kc8Aeug.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4NIgoL675gbpcE9OYAoqjlW4ttg1KlrdJunzGJ2D6QzDa7C5kc8Aeug\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"830\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4e0b\u8f7d\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(celldex)<\/span><\/code><code><span class=\"code-snippet_outer\">ref <- celldex::HumanPrimaryCellAtlasData() <span class=\"code-snippet__comment\">#\u4e0b\u8f7d\u4eba\u7c7b\u7ec6\u80de\u6570\u636e<\/span><\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u4eca\u5929\u4f7f\u7528\u7684\u6848\u4f8b\u662f\u7528\u5916\u5468\u8840\u7ec6\u80de\u53ef\u4ee5\u7528Monaco Inunene Data\u3002\u76ee\u524d singler \u5df2\u7ecf\u5c06\u8fd9\u4e9b\u6570\u636e\u5e93\u6253\u5305\u5230 celldex \u5305\u4e2d\uff0c\u53ef\u4ee5\u901a\u8fc7\u5b89\u88c5 celldex \u5305\u8fdb\u884c\u5b89\u88c5\u548c\u4e0b\u8f7d\u3002\u4e0b\u8f7d\u7684\u6570\u636e\u4e3a SummarizedExperiment \u7c7b\u5bf9\u8c61\uff0c\u53ef\u4ee5\u901a\u8fc7\u6dfb\u52a0 Ensemble=TRUE\uff0c\u4e0b\u8f7d Ensemble \u7c7b\u578b\u57fa\u56e0 ID\uff0c\u901a\u8fc7\u6dfb\u52a0 cell.ont = c(“all”, “nonna”, “none”) \u6dfb\u52a0 Cell Ontology terms \u4fe1\u606f\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3924170616113744\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/5_p3cib8FicDCXPKdD8oeUwEF17npBQA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4O6DzIBWnv6oPHLjBUVyBico1JibMp3cib8FicDCXPKdD8oeUwEF17npBQA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1055\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"color: rgb(16, 116, 231);padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\uff08\u4e09\uff09\u53c2\u8003\u57fa\u56e0\u521d\u63a2<\/strong><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.45695364238410596\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/6_NicFPap7yvrDYjm2tBamzDBsyFd25Q.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4wkZYUZg5QLyiaj9Aib8Qms8fdUuNicFPap7yvrDYjm2tBamzDBsyFd25Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"755\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u5217\u662f\u6bcf\u4e2a\u7ec6\u80de\uff0c\u5171713\u79cd\u3002<\/p>\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e8c\u3001SingleR\u6ce8\u91ca<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><span style=\"color: rgb(16, 116, 231);\"><strong>\uff08\u4e00\uff09\u8bfb\u53d6\u6570\u636e<\/strong><\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6seurat\u5305\u5206\u6790\u7684\u5355\u7ec6\u80de\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc.data <- readRDS(\u201cxxx.rds<span class=\"code-snippet__string\">\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">#\u83b7\u5f97\u5355\u7ec6\u80de\u8868\u8fbefeature_barcode\u77e9\u9635<\/span><\/code><code><span class=\"code-snippet_outer\">query <- pbmc@assays$RNA@data<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.42276422764227645\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/7_ROY61ajLnJWBw7nVYZfEK5eojo4vUw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4YWzVfFW8xLB6Q8ibCfwkMAjwnROY61ajLnJWBw7nVYZfEK5eojo4vUw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"246\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u901a\u8fc7\u67e5\u770b\u53ef\u4ee5\u53d1\u73b0\u662f\u77e9\u9635\u683c\u5f0f\u3002<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.28393881453154873\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/8_rAyA5Xansib31W2qlP0ZlE0LtZDWqw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4bTNiaQSFASHwFV5RaR2HXW6eYGrAyA5Xansib31W2qlP0ZlE0LtZDWqw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1046\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u884c\u540d\u662f\u57fa\u56e0\uff0c\u5217\u662f\u5355\u7ec6\u80de\u7684barcode\u7684\u4e00\u4e2a\u7a00\u758f\u77e9\u9635\u3002<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><span style=\"color: rgb(16, 116, 231);\"><strong>\uff08\u4e8c\uff09\u6ce8\u91ca\u7ec6\u80de\u540d<\/strong><\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#SingleR\u6ce8\u91ca<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pred.pbmc<-SingleR(test=query,assay.type.test=<span class=\"code-snippet__number\">1<\/span>,ref=ref,clusters=pbmc@active.ident,labels=ref$label.fine)<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;border-width: 1px;border-radius: 8px;border-style: dotted;border-color: rgb(0, 0, 0);overflow: hidden;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin: 15px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);padding-right: 15px;padding-left: 15px;\">\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">Test \u8f93\u5165\u8868\u8fbe\u77e9\u9635<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">Ref \u4e0b\u8f7d\u597d\u7684\u53c2\u8003\u6570\u636e<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">Cluster \u7ec6\u80de\u5206\u7fa4\u6570<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">Lab \u4f7f\u7528\u6570\u636e\u81ea\u5e26\u7684\u7ec6\u80de\u540d<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.4305555555555556\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/9_2uSsDOAibw5ojcV8KeibmbDnnF8ZVw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4GXwueQDSM7cs6jUH6TIpWfGs2b2uSsDOAibw5ojcV8KeibmbDnnF8ZVw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u5177\u4f53\u8be6\u7ec6\u7684\u53c2\u6570\u4fe1\u606f\uff0c\u53ef\u4ee5\u8f93\u5165\uff1fSingleR\u67e5\u770b\u5e2e\u52a9\u6587\u6863<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.19722222222222222\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/10_icrtOpMZSI3Jklric8icv7gAj4B7dg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4nLQvGL01HPcHS8GQ9P6uwSmCpGCicrtOpMZSI3Jklric8icv7gAj4B7dg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u6ce8\u91ca\u597d\u7684\u6570\u636e<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u67e5\u770b\u6ce8\u91ca\u7ed3\u679c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">View(as.data.frame(pred.pbmc))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8f93\u51fa\u6ce8\u91ca\u7684\u7ec6\u80de<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pred.pbmc$pruned.labels<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.08981481481481482\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/11_jibxqJgibPeiamib1ltz1pJv0qfmCQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4F639mGF3FVWjw2vTEREn9yR1riaIRjibxqJgibPeiamib1ltz1pJv0qfmCQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ec6\u80de\u7c7b\u522b\u7edf\u8ba1<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">table(pred.pbmc$pruned.labels)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.20462962962962963\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/12_XSrH63hCZvNOpjnAUtogSy1PCKzvgg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4NQJRNtKiaxhQoa5IIeI51O6p9XSrH63hCZvNOpjnAUtogSy1PCKzvgg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u5171\u8ba17\u4e2a\u7ec6\u80de\u7c7b<\/p>\n<p><br \/><\/p>\n<p><span style=\"color: rgb(16, 116, 231);\"><strong>\uff08\u4e09\uff09\u4f7f\u7528\u6ce8\u91ca\u7684\u7ec6\u80de\u540d\u79f0<\/strong><\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u521b\u5efa\u4e00\u4e2a\u65b0\u7684\u53d8\u91cf\u50a8\u5b58\u7ec6\u80de\u540d\u79f0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">new.cluster.ids <- pred.pbmc$labels<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.15568022440392706\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/13_qeM4sQUGjpov8NbXhVysse1uPibuBw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4TzQGiaXf2LN0fOdhSLly6OEzUdqeM4sQUGjpov8NbXhVysse1uPibuBw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"713\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>9\u79cd\u7ec6\u80de\u540d\u79f0<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u66ff\u6362level\u4e3a\u7ec6\u80de\u540d\uff0c\u5c06level\u4ece\u6570\u5b57\u66ff\u6362\u6210\u7ec6\u80de\u540d\u79f0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">names<\/span>(new.cluster.ids) <- levels(pbmc)<\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- RenameIdents(pbmc,new.cluster.ids)<\/span><\/code><code><span class=\"code-snippet_outer\">levels(pbmc)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.19536903039073805\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/14_ty3Y7q7BibcbJ98AKSnP3cSTdtoLbA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx47K6yMEaISrN2rbvRLOWddqKXUty3Y7q7BibcbJ98AKSnP3cSTdtoLbA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"691\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong style=\"color: rgb(16, 116, 231);\">\uff08\u56db\uff09\u7ed8\u56fe<\/strong><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u67e5\u770b\u6ce8\u91ca\u7ed3\u679c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">plotScoreHeatmap(pred.pbmc)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7194444444444444\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/15_yXoTdp6yZcQ41c9v29XfJiaxp0Z0zw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4B1kC13jjqZ8KwIMXqDD2n960byXoTdp6yZcQ41c9v29XfJiaxp0Z0zw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u7531\u7d2b\u5230\u9ec4\uff0c\u76f8\u5173\u6027\u4ece\u4f4e\u5230\u9ad8\u3002\u5206\u6210\u4e86\u5176\u4e2d\u7ec6\u80de\u3002<\/p>\n<p><br \/><\/p>\n<p><br \/><\/p>\n<p><span style=\"color: rgb(16, 116, 231);\"><strong>\uff08\u4e94\uff09\u4e3a\u7ec6\u80de\u7fa4\u6dfb\u52a0\u6ce8\u91ca<\/strong><\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236umap\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">DimPlot(pbmc, reduction = <span class=\"code-snippet__string\">\"umap\"<\/span>, label = <span class=\"code-snippet__literal\">TRUE<\/span>, pt.size = <span class=\"code-snippet__number\">0.5<\/span>) + NoLegend()<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9976608187134502\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/16_ia2zfVsT8csYlfwP72P3XYaNU1Kvjw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4Iyk0SvKGqrcQMyA5s8d3ia7ws6ia2zfVsT8csYlfwP72P3XYaNU1Kvjw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"855\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236t-SNE\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">DimPlot(pbmc, reduction = <span class=\"code-snippet__string\">\"tsne\"<\/span>, label = <span class=\"code-snippet__literal\">TRUE<\/span>, pt.size = <span class=\"code-snippet__number\">0.5<\/span>) + NoLegend()<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9849012775842044\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/17_bXqsbOQMj9JANlRHZhtaUyysMItdCw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx4d0gnyhTBDBZXNINrXPR6PZdwibXqsbOQMj9JANlRHZhtaUyysMItdCw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"861\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section 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\/><\/p>\n<p>\u6b22\u8fce\u4f7f\u7528\uff1a\u4e91\u751f\u4fe1 – \u5b66\u751f\u7269\u4fe1\u606f\u5b66 (biocloudservice.com)<\/p>\n<p><br \/><\/p>\n<p>\u5982\u679c\u60f3\u7528\u670d\u52a1\u5668\u53ef\u4ee5\u8054\u7cfb\u5fae\u4fe1\uff1a18502195490\uff08\u5feb\u6765\u8054\u7cfb\u6211\u4eec\u4f7f\u7528\u5427\uff01\uff09<\/p>\n<\/section>\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row;transform: translate3d(40px, 0px, 0px);margin-bottom: -40px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 26px;height: 26px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);border-left: 1px solid rgb(160, 160, 160);border-bottom-left-radius: 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650761303&idx=2&sn=6f73471d1e82c56c0b2160d0fcd32d53&scene=21#wechat_redirect\" textvalue=\"\" linktype=\"image\" imgurl=\"https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/0?wx_fmt=jpeg\" imgdata=\"[object Object]\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.35083798882681566\" data-s=\"300,640\" 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onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSbGdkTKlXI3tbocicP8GUCzibJV5ibjQs6D7lQhs1pnDYZQiauBwGGHoIUWPWSIgBgzuWA7zggZhslM2g\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"900\" style=\"\" \/><\/p>\n<p powered-by=\"xiumi.us\">\uff08\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u8df3\u8f6c\uff09<\/p>\n<section style=\"text-align: left;margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;min-width: 10%;vertical-align: top;padding-right: 20px;\">\n<section style=\"transform: translate3d(20px, 0px, 0px);margin: 10px 0% -30px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);text-align: justify;font-size: 12px;\">\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.866\" data-s=\"300,640\" 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wxw-img\" data-ratio=\"0.7709090909090909\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u300c\u8f6c\u300d3\u5206\u949f\u6559\u4f60\u4f7f\u7528SingleR\u5305\u81ea\u52a8\u5316\u9274\u5b9a\u5355\u7ec6\u80de\u4e9a\u7fa4\/21_Ofd5wibutTPvibZFEokSFMKwYmIwFg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSbFZum7bxPBLwl2uKYwWBx44n05H7orkebwoRBtqVyh1FohtfOfd5wibutTPvibZFEokSFMKwYmIwFg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"275\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" 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