{"id":54138,"date":"2024-06-16T13:42:08","date_gmt":"2024-06-16T05:42:08","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=54138"},"modified":"2024-06-16T13:42:08","modified_gmt":"2024-06-16T05:42:08","slug":"%e6%96%b0%e6%89%8b%e4%b8%8a%e8%b7%af%ef%bc%8c%e7%bb%88%e4%ba%8e%e8%b7%91%e9%80%9a%e4%ba%86%ef%bc%9a%e5%88%a9%e7%94%a8edta%e6%b3%a8%e9%87%8a%e5%9f%ba%e5%9b%a0%e7%bb%84%e7%9a%84%e9%87%8d%e5%a4%8d-2","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=54138","title":{"rendered":"\u65b0\u624b\u4e0a\u8def\uff0c\u7ec8\u4e8e\u8dd1\u901a\u4e86\uff1a\u5229\u7528edta\u6ce8\u91ca\u57fa\u56e0\u7ec4\u7684\u91cd\u590d\u5e8f\u5217\uff08\u4ee5\u62df\u5357\u82a5\u4e3a\u4f8b\uff09"},"content":{"rendered":"


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rich_media_meta icon_appmsg_tag appmsg_title_tag weui-wa-hotarea\">\u539f\u521b  <\/span><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u679c  <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c  <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-05-12 19:00:16<\/em><\/div>\n<p><content><\/p>\n<section data-role=\"outer\" label=\"edit by 135editor\">\n<section style=\"letter-spacing: 0.578px;font-size: 16px;\">\n<section>\n<section>\n<section>\n<section>\n<section>\n<section data-width=\"100%\" style=\"width: 100%;flex: 0 0 100%;opacity: 1;\">\n<section style=\"line-height: 1.8;\">\n<section>\n<section>\n<section style=\"margin-top: 10px;margin-bottom: 20px;display: flex;flex-flow: row nowrap;text-align: center;justify-content: center;\">\n<section style=\"display: inline-block;width: 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15px;\">\u7acb\u523b\u62e5\u6709\u4e00\u4e2aRstudio\u8d26\u53f7<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u5f00\u542f\u5347\u7ea7\u6a21\u5f0f\u5427<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"color: rgb(255, 129, 36);font-size: 12px;letter-spacing: 0.5px;\">\uff0856\u7ebf\u7a0b\uff0c256G\u5185\u5b58\uff0c\u4e2a\u4eba\u5b58\u50a81T\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;width: 30%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 30% !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"162\" data-cropsely1=\"0\" data-cropsely2=\"162\" data-ratio=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_ib2l1ye6HNIkwTG696C2pf9yAtdeGA-24.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAyB69UyXDLG0wQcISNwmL6DlpIKVBnCSOXHTWJhLcxic2qtibaib2l1ye6HNIkwTG696C2pf9yAtdeGA\/640?wx_fmt=png'\" data-type=\"jpeg\" data-w=\"400\" style=\"height: 111px;vertical-align: middle;width: 111px;\" data-recalc-dims=\"1\"><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section data-role=\"list\">\n<ul class=\"list-paddingleft-1\" style=\"padding-left: 30px;width: 100%;list-style-position: outside;\">\n<li>\n<p style=\"text-align:justify;text-indent: 0em;line-height: 1.75em;\"><span style=\"font-size: 14px;letter-spacing: 0.5px;line-height: 2em;\"><span style=\"font-family: 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0);\">\u5f53\u5929\u771f\u7684\u5c0f\u679c\u4ee5\u4e3a\u88c5\u597d\u8f6f\u4ef6\u5bf9\u4ee3\u7801\u590d\u5236\u7c98\u8d34\uff08\u4e0d\u662f\uff09\u5c31\u53ef\u4ee5\u8f7b\u677e\u6ce8\u91ca\u65f6\uff0c\u60b2\u5267\u5c31\u8bde\u751f\u4e86\uff01\u56e0\u4e3a\u4f5c\u4e3a\u4e00\u53ea\u83dc\u679c\uff0c\u5979\u8fd8\u4e0d\u660e\u767derro\uff01erro\uff01error\uff01\u662f\u80fd\u628a\u4eba\u903c\u75af\u7684\u3002<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">\u5e9f\u8bdd\u4e0d\u591a\u8bf4\uff0c\u6211\u4eec\u8fd8\u662f\u8fdb\u5165\u6b63\u9898\u5427\uff01\u4e0a\u671f\u8bf4\u8981\u7528\u5230\u5c0f\u9f20\u57fa\u56e0\u7ec4\uff0c\u4f46\u662f\u8981\u7ec3\u4e60\u7684\u8bdd\u8fd8\u662f\u9009\u62e9\u57fa\u56e0\u7ec4\u6bd4\u8f83\u5c0f\u7684\u7269\u79cd\u66f4\u5feb\uff0c\u6240\u4ee5\u5c0f\u679c\u6362\u6210\u4e86\u62df\u5357\u82a5\u3002<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">1. \u4e0b\u8f7d\u57fa\u56e0\u7ec4\u6570\u636e\u5e76\u89e3\u538b\u7f29Arabidopsis thaliana genome assembly TAIR10.1 – NCBI – NLM (nih.gov)<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\"> <img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.35478260869565215\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_R1QF0TvkFicT03F8wXjE2jHoibmbVw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAyrricjRoBoPO5X3NaJyYDOb3okRAvJsFos6iahrbicg5XrloDgOR1QF0TvkFicT03F8wXjE2jHoibmbVw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"575\" style=\"vertical-align:inherit;\" data-recalc-dims=\"1\"><\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">\u5728ncbi\u63d0\u4f9b\u4e863\u79cd\u65b9\u5f0f\uff0cdownload\u53ef\u4ee5\u76f4\u63a5\u4e0b\u8f7d\u5230\u672c\u5730\uff0cdatasets\u5219\u8981\u4e0b\u8f7dncbi datasets\u8fdb\u884c\u5b89\u88c5\uff0ccurl\u5219\u9700\u8981\u5df2\u5b89\u88c5curl\uff0c\u6bd4\u5982\u8bf4\u7528curl\u5b89\u88c5\u5219\u76f4\u63a5\u590d\u5236\u547d\u4ee4\u5230\u7ec8\u7aef\u5c31\u53ef\u4ee5\u4e86\u3002<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\"> <img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.4209245742092457\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_g039gtsjR1KMoWqlRgQYGqvucAoZ9A.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAyrricjRoBoPO5X3NaJyYDObhvXED87jDWibm5eWUl8cZ7B06g039gtsjR1KMoWqlRgQYGqvucAoZ9A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"411\" style=\"vertical-align:inherit;\" data-recalc-dims=\"1\"><\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">\u5728\u8fc7\u7a0b\u4e2d\u9700\u8981\u7528\u5230genometools\uff0c\u6211\u4eec\u7528miniconda\u5b89\u88c5\u5373\u53ef\uff0c\u4e0d\u6307\u5b9a\u5b89\u88c5\u76ee\u5f55\u4f1a\u9ed8\u8ba4\u5b89\u88c5\u5230conda\u73af\u5883\u4e0b\u7684pkgs\u76ee\u5f55\u4e0b\u3002<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">conda install -c bioconda genometools-genometools<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">\u67e5\u770b\u5b89\u88c5\u60c5\u51b5<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">conda list genometools<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">4. \u5f00\u59cb\u6ce8\u91ca\uff01<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">conda activate EDTA<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">nohup perl ~\/EDTA\/EDTA.pl <\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–genome ~\/my_folder\/GCF_000001735.4\/GCF_000001735.4_TAIR10.1_genomic.fna <\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–species others <\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–step all <\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–overwrite 1 <\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–cds ~\/my_folder\/GCF_000001735.4\/cds_from_genomic.fna <\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–sensitive 1 <\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–anno 1 <\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–evaluate 1 <\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–force 1 <\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–u 1.3e-8 <\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–threads 4 <\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–debug 0 <\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">>output.log &<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">\u4e3a\u4e86\u66f4\u597d\u7406\u89e3\uff0c\u5c0f\u679c\u5c06\u4ee3\u7801\u7684\u542b\u4e49\u653e\u5728\u4e0b\u9762\uff1a<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">\u8bb0\u5f97\u5148\u6fc0\u6d3bEDTA\u73af\u5883\u54e6\uff01<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">nohup: \u5ffd\u7565SIGHUP\u4fe1\u53f7\uff0c\u5373\u4f7f\u5f53\u524d\u7ec8\u7aef\u5173\u95ed\u4e5f\u80fd\u7ee7\u7eed\u8fd0\u884c\u3002<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–genome\uff1a\u6307\u5b9a\u5f85\u5206\u6790\u7684\u57fa\u56e0\u7ec4\u5e8f\u5217\u6587\u4ef6\u8def\u5f84<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–species\uff1a\u6307\u5b9a\u5f85\u5206\u6790\u57fa\u56e0\u7ec4\u7684\u7269\u79cd\u4fe1\u606f\uff0c\u8fd9\u91cc\u6307\u5b9a\u4e3a\u201cothers\u201d<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–step\uff1a\u6307\u5b9a\u5206\u6790\u6b65\u9aa4\uff0c\u8fd9\u91cc\u6307\u5b9a\u4e3a\u201call\u201d\u8868\u793a\u6267\u884c\u5b8c\u6574\u7684\u5206\u6790\u6d41\u7a0b<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–overwrite\uff1a\u5982\u679c\u8f93\u51fa\u6587\u4ef6\u5df2\u7ecf\u5b58\u5728\uff0c\u662f\u5426\u8986\u76d6\uff0c\u8fd9\u91cc\u6307\u5b9a\u4e3a\u201c1\u201d\u8868\u793a\u8986\u76d6<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–cds\uff1a\u6307\u5b9a\u5305\u542bCDS\u5e8f\u5217\u7684\u6587\u4ef6\u8def\u5f84\uff0c\u8fd9\u4e2a\u6587\u4ef6\u901a\u5e38\u7531\u57fa\u56e0\u7ec4\u6ce8\u91ca\u6587\u4ef6\u63d0\u4f9b<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–sensitive\uff1a\u662f\u5426\u4f7f\u7528\u8f83\u4e3a\u654f\u611f\u7684TE\u8bc6\u522b\u7b97\u6cd5\uff0c\u8fd9\u91cc\u6307\u5b9a\u4e3a\u201c1\u201d\u8868\u793a\u4f7f\u7528<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–anno\uff1a\u662f\u5426\u4f7f\u7528\u5df2\u6709\u7684\u57fa\u56e0\u7ec4\u6ce8\u91ca\u4fe1\u606f\u8fdb\u884c\u5206\u6790\uff0c\u8fd9\u91cc\u6307\u5b9a\u4e3a\u201c1\u201d\u8868\u793a\u4f7f\u7528<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–evaluate\uff1a\u662f\u5426\u5bf9\u5206\u6790\u7ed3\u679c\u8fdb\u884c\u8bc4\u4f30\uff0c\u8fd9\u91cc\u6307\u5b9a\u4e3a\u201c1\u201d\u8868\u793a\u4f7f\u7528<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–force\uff1a\u662f\u5426\u5f3a\u5236\u6267\u884c\uff0c\u8fd9\u91cc\u6307\u5b9a\u4e3a\u201c1\u201d\u8868\u793a\u5f3a\u5236\u6267\u884c<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–u\uff1a\u6307\u5b9aTE\u5e93\u4e2d\u7684\u6700\u5c0f\u4e0d\u540c\u79cd\u540c\u65cf\u91cd\u590d\u5143\u4ef6\u6570\u91cf\uff0c\u8fd9\u91cc\u6307\u5b9a\u4e3a\u201c1.3e-8\u201d<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–threads\uff1a\u6307\u5b9a\u4f7f\u7528\u7684\u7ebf\u7a0b\u6570\u91cf\uff0c\u8fd9\u91cc\u6307\u5b9a\u4e3a\u201c4\u201d<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">–debug\uff1a\u662f\u5426\u8f93\u51fa\u8c03\u8bd5\u4fe1\u606f\uff0c\u8fd9\u91cc\u6307\u5b9a\u4e3a\u201c0\u201d\u8868\u793a\u4e0d\u8f93\u51fa<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">edta.log\uff1a\u5c06\u6807\u51c6\u8f93\u51fa\u91cd\u5b9a\u5411\u5230\u540d\u4e3a\u201cedta.log\u201d\u7684\u6587\u4ef6\u4e2d<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">&\uff1a\u5c06\u8fdb\u7a0b\u653e\u5230\u540e\u53f0\u8fd0\u884c\u3002<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\"> <img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.37398373983739835\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_OFRkLJuVS5KWj0USsXmHfclmlibFgg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAyrricjRoBoPO5X3NaJyYDOb6fwNy9gicrvRXvG6JLxecOvVO8OFRkLJuVS5KWj0USsXmHfclmlibFgg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"492\" style=\"vertical-align:inherit;\" data-recalc-dims=\"1\"><\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">\u63a5\u4e0b\u6765\u53ef\u4ee5\u901a\u8fc7\u67e5\u770b\u8f93\u51fa\u65e5\u5fd7\u6765\u67e5\u770b\u8fdb\u5ea6<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">cat output.log<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\"> <img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.39119804400978\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_pJUza9icUmGrSiaTWQks46qBia1CoA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAyrricjRoBoPO5X3NaJyYDObzftM9Dz2GasFLgbTNUJ4QtKGrxypJUza9icUmGrSiaTWQks46qBia1CoA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"409\" style=\"vertical-align:inherit;\" data-recalc-dims=\"1\"><\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">\u53ef\u4ee5\u770b\u5230\u5c0f\u679c\u7684\u8fdb\u5ea6\u76ee\u524d\u662f\u6ca1\u6709erro\u7684~<\/span><\/p>\n<p><br \/><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">\u5c0f\u679c\u5728\u8fd9\u4e00\u8fc7\u7a0b\u4e2d\u72af\u4e86\u4e00\u4e9b\u9519\u8bef\uff0c\u7ed9\u5927\u5bb6\u501f\u9274\uff1a<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">1.\u5fd8\u8bb0\u6fc0\u6d3bEDTA \u73af\u5883\u5bfc\u81f4\u62a5\u9519<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">2.\u6ca1\u6709\u5b89\u88c5genometools<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">3.\u8def\u5f84\u9519\u8bef<\/span><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">\u5f53\u7136\u4e86\uff0c\u6bcf\u4e2a\u4eba\u9047\u5230\u7684\u95ee\u9898\u53ef\u80fd\u4e0d\u4e00\u6837\uff0c\u5728\u8fc7\u7a0b\u4e2d\u53ef\u4ee5\u6839\u636e\u81ea\u5df1\u7684\u62a5\u9519\u6765\u89e3\u51b3\u95ee\u9898\u3002<\/span><\/p>\n<p><br \/><\/p>\n<p>\u6b22\u8fce\u4f7f\u7528\uff1a<em style=\"letter-spacing: 0.578px;color: rgb(0, 0, 0);font-size: 17px;line-height: 1.59em;\"><strong>\u4e91\u751f\u4fe1  – \u5b66\u751f\u7269\u4fe1\u606f\u5b66 (biocloudservice.com)<\/strong><\/em><\/p>\n<p>\u5982\u679c\u60f3\u7528\u670d\u52a1\u5668\u53ef\u4ee5\u8054\u7cfb\u5fae\u4fe1\uff1a<em style=\"letter-spacing: 0.578px;color: rgb(0, 0, 0);font-size: 17px;line-height: 1.59em;\"><strong>18502195490\uff08\u5904\u7406\u5927\u91cf\u6570\u636e\u7684\u65f6\u5019\u4e0d\u7528\u670d\u52a1\u5668\u7528\u4ec0\u4e48\uff01\u5feb\u6765\u8054\u7cfb\u6211\u4eec\u4f7f\u7528\u5427\uff01\uff09<\/strong><\/em><\/p>\n<section style=\"height: 0px;overflow: hidden;\"><br \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-align:center;text-indent: 0em;line-height: 1.75em;\"><br \/><\/p>\n<section data-tools=\"135\u7f16\u8f91\u5668\" data-id=\"105054\" style=\"font-size: 16px;\">\n<section style=\"margin: 10px auto;text-align: center;\">\n<section style=\"padding-top: 30px;padding-bottom: 30px;background-color: rgb(242, 246, 255);border-radius: 7px;\">\n<section style=\"display: flex;justify-content: space-evenly;align-items: center;\">\n<section data-width=\"26%\" style=\"width: 26%;max-width: 26% !important;\">\n<section style=\"padding: 7px;border-radius: 15px;box-shadow: rgba(169, 187, 223, 0.45) 4px 4px 8px, rgb(255, 255, 255) -2.5px -2.5px 5px;\">\n<section data-width=\"100%\" style=\"width: 100%;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.005281690140845\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_bCYIDZfwLuT4nrseE4TicyG6FGPicg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAyrricjRoBoPO5X3NaJyYDObrubfmmLA3icGIDxH3fHl1JG7wzGbCYIDZfwLuT4nrseE4TicyG6FGPicg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"568\" data-width=\"100%\" style=\"vertical-align: inherit;width: 100%;display: block;border-radius: 7px;\" title=\"\u5c0f\u4e91-\u5fae\u4fe1\u4e8c\u7ef4\u7801.png\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section data-width=\"62%\" style=\"width: 62%;max-width: 62% !important;\">\n<section style=\"display: inline-block;\">\n<section style=\"padding: 7px 15px;border-radius: 20px;box-shadow: rgba(169, 187, 223, 0.45) 6px 6px 8px inset, rgb(255, 255, 255) -2.5px -2.5px 5px inset;\">\n<section data-brushtype=\"text\" style=\"font-size: 14px;letter-spacing: 1.5px;color: rgb(111, 133, 172);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u5fae\u4fe1\u53f7 | 18502195490<\/span><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-top: 12px;padding: 7px 15px;border-radius: 20px;box-shadow: rgba(169, 187, 223, 0.45) 6px 6px 8px inset, rgb(255, 255, 255) -2.5px -2.5px 5px inset;\">\n<section data-brushtype=\"text\" style=\"font-size: 14px;letter-spacing: 1.5px;color: rgb(111, 133, 172);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u77e5\u4e4e | \u751f\u4fe1\u679c<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"letter-spacing: 0.578px;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-top: 0.5em;\">\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);\">\n<section style=\"text-align: left;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;\">\n<section style=\"margin-bottom: 8px;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u70b9\u51fb<strong><span style=\"color: rgb(0, 150, 136);\">\u201c\u9605\u8bfb\u539f\u6587\u201d<\/span><\/strong>\u7acb\u523b\u62e5\u6709<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 136);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u2193\u2193\u2193 <\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br 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