<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 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solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"xCell\uff1a\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u7684\u514d\u75ab\u6d78\u6da6\u5206\u6790\u4e13\u5bb6\uff01\",\"time\":\"2023-05-16 19:01:06\",\"timeStamp\":\"1684234866\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247487597&idx=1&sn=32201aa83df9d8b7cc9294a1a5bcf8a5&chksm=cf7c80a8f80b09bebacd1c8145f0bde2e139cebbb4cdea84417ab31d1e81985297314e0fa188#rd\" target=\"_blank\" rel=\"noopener\">xCell\uff1a\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u7684\u514d\u75ab\u6d78\u6da6\u5206\u6790\u4e13\u5bb6\uff01<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span id=\"copyright_logo\" class=\"wx_tap_link js_wx_tap_highlight rich_media_meta icon_appmsg_tag appmsg_title_tag weui-wa-hotarea\">\u539f\u521b <\/span><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u679c <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-05-16 19:01:06<\/em><\/div>\n<p><content><\/p>\n<section data-role=\"outer\" label=\"edit by 135editor\">\n<section style=\"letter-spacing: 0.578px;font-size: 16px;\">\n<section>\n<section>\n<section>\n<section>\n<section>\n<section data-width=\"100%\" style=\"width: 100%;flex: 0 0 100%;opacity: 1;\">\n<section style=\"line-height: 1.8;\">\n<section>\n<section>\n<section style=\"margin-top: 10px;margin-bottom: 20px;display: flex;flex-flow: row nowrap;text-align: center;justify-content: center;\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;border-style: solid;border-width: 1px;border-color: rgb(67, 67, 67);flex: 0 0 auto;align-self: flex-start;height: auto;box-shadow: rgb(255, 219, 118) 6px 6px 0px;background-color: rgb(255, 255, 255);\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 70%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 70% !important;\">\n<section style=\"margin-top: 10px;\">\n<section style=\"text-align: center;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u751f\u4fe1\u4ebaR\u8bed\u8a00\u5b66\u4e60\u5fc5\u5907<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u7acb\u523b\u62e5\u6709\u4e00\u4e2aRstudio\u8d26\u53f7<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u5f00\u542f\u5347\u7ea7\u6a21\u5f0f\u5427<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"color: rgb(255, 129, 36);font-size: 12px;letter-spacing: 0.5px;\">\uff0856\u7ebf\u7a0b\uff0c256G\u5185\u5b58\uff0c\u4e2a\u4eba\u5b58\u50a81T\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;width: 30%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 30% !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"162\" data-cropsely1=\"0\" data-cropsely2=\"162\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_ib2l1ye6HNIkwTG696C2pf9yAtdeGA-25.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAyB69UyXDLG0wQcISNwmL6DlpIKVBnCSOXHTWJhLcxic2qtibaib2l1ye6HNIkwTG696C2pf9yAtdeGA\/640?wx_fmt=png'\" data-type=\"jpeg\" style=\"height: 111px;vertical-align: middle;width: 111px;\" data-ratio=\"1\" data-w=\"400\" data-recalc-dims=\"1\" \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section data-role=\"list\">\n<ul class=\"list-paddingleft-1\" style=\"padding-left: 30px;width: 100%;list-style-position: outside;\">\n<li>\n<section><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">hi\u5927\u5bb6\uff0c\u597d\u4e45\u4e0d\u89c1\uff0c\u5c0f\u679c\u53c8\u6765\u5566\uff01<\/span><\/section>\n<\/li>\n<li>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">xCell\u662f\u4e00\u79cd\u57fa\u4e8e\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u7684\u514d\u75ab\u7ec6\u80de\u6d78\u6da6\u8bc4\u4f30\u5de5\u5177\uff0c\u53ef\u4ee5\u8bc6\u522b\u51fa\u6f5c\u5728\u7684\u514d\u75ab\u7ec6\u80de\u4e9a\u7fa4\u5e76\u8ba1\u7b97\u5b83\u4eec\u5728\u7ec4\u7ec7\u4e2d\u7684\u76f8\u5bf9\u4e30\u5ea6\u3002\u5b83\u53ef\u4ee5\u5e7f\u6cdb\u5e94\u7528\u4e8e\u764c\u75c7\u3001\u81ea\u8eab\u514d\u75ab\u6027\u75be\u75c5\u548c\u5176\u4ed6\u75be\u75c5\u7684\u514d\u75ab\u6d78\u6da6\u5206\u6790\u4e2d\u3002<\/span><\/p>\n<\/li>\n<\/ul>\n<section>\n<section>\n<section><br \/><\/section>\n<section style=\"font-size: 16px;\">\n<section style=\"margin-top: 5px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4eca\u5929\u5c0f\u679c\u5c31\u6765\u6559\u5927\u5bb6\u5982\u4f55\u5b9e\u6218\u5e94\u7528xCell\u8fdb\u884c\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u7684\u514d\u75ab\u6d78\u6da6\u5206\u6790\uff0c\u90a3\u5c31\u548c\u5c0f\u679c\u4e00\u8d77\u6765\u770b\u4e0b\u5427\uff01<\/span><\/p>\n<\/section>\n<section style=\"line-height: 0;text-align: center;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_QiaOCcR0jFpvjkSAu3UmKL82ohCV5Q-1.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNXhQ4GD7nqK0jxIA9vNfaZMfcibQiaOCcR0jFpvjkSAu3UmKL82ohCV5Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"500\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section style=\"margin-top: 5px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<section>\n<p style=\"text-align: center;white-space: normal;\"><em><strong><span style=\"font-size: 20px;\">\u6570\u636e\u9884\u5904\u7406<\/span><\/strong><\/em><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4e0b\u8f7dxCell\u5305\u5e76\u5bfc\u5165\uff1a<\/span><\/p>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">'devtools'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">devtools::install_github(<span class=\"code-snippet__string\">'dviraran\/xCell'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">library(xCell)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"line-height: 0;text-align: center;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_s2ZHCtbN9nDKgpfniawYJJzqxT6v6g.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNq0nuSrPNnVriasrjcop8ONDW3ss2ZHCtbN9nDKgpfniawYJJzqxT6v6g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"500\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section style=\"margin-top: 5px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<section>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;color: rgb(62, 62, 62);\">xCell\u514d\u75ab\u6d78\u6da6\u5206\u6790<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5bfc\u5165\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\uff0c\u5e76\u901a\u8fc7xCellAnalysis\u5206\u6790\u8868\u8fbe\u77e9\u9635\uff0c\u8be5\u51fd\u6570\u7528\u4e8e\u9884\u6d4b\u7ec6\u80de\u7ec4\u5206\u5728\u7ed9\u5b9a\u6837\u672c\u4e2d\u7684\u76f8\u5bf9\u4e30\u5ea6\uff1a<\/span><\/p>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">exprSet=read.csv(<span class=\"code-snippet__string\">\"GSE57065_ExprMatrix.csv\"<\/span>,header=T,row.names=<span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">xCell <- xCellAnalysis(exprSet) ##<span class=\"code-snippet__meta\">#array data<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">write.csv(xCell,<span class=\"code-snippet__string\">\"xCell_score.csv\"<\/span>) #\u5c06\u8ba1\u7b97\u540e\u7684\u7ed3\u679c\u5199\u5165\u8868\u683c\u6587\u4ef6\u4e2d<\/span><\/code><\/pre>\n<\/section>\n<section style=\"margin-top: 5px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5148\u6765\u4e00\u8d77\u770b\u4e0b\u5206\u6790\u540e\u7684\u6570\u636e\u96c6\u5427\uff01<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6371841155234657\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_fUBIIH3vsVekJbmH1KnO48DII995DA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNcJeIyg3dEFnePCe7O2ib4bGcUfUBIIH3vsVekJbmH1KnO48DII995DA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;text-align: center;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.49899799599198397\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_PSgMgn9cx3xIMRpoK7HUMUxLr0HL3A.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNszG7picpYriceh3kdysIicAjyUtPSgMgn9cx3xIMRpoK7HUMUxLr0HL3A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"499\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section style=\"margin-top: 5px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<section>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;color: rgb(62, 62, 62);\">\u5904\u7406xCell\u5206\u6790\u540e\u7684\u7ed3\u679c\u6570\u636e\u96c6<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u53ef\u4ee5\u6839\u636e\u6837\u672c\u7684\u5206\u7ec4\u60c5\u51b5\uff0c\u63d0\u53d6\u51fa\u5bf9\u5e94\u7684\u7ec6\u80de\u7c7b\u578b\u5f97\u5206\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u9996\u5148\u6211\u4eec\u8981\u8bfb\u53d6 group.txt \u7684\u6587\u4ef6\uff0c\u8fd9\u91cc\u5305\u542b\u4e86\u6837\u672c\u7684\u5206\u7ec4\u4fe1\u606f\uff0c\u6e29\u99a8\u63d0\u793a\uff0c\u7ec6\u80de\u8868\u8fbe\u6837\u672c\u6570\u636e\u5305\u542b\u4e24\u79cd\u7c7b\u578b\u54e6\uff01<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u6211\u4eec\u53ef\u4ee5\u8bfb\u5165\u8fd9\u4e2a\u6587\u4ef6\uff0c\u5e76\u63d0\u53d6\u51fa\u201cseptic_shock\u201d \u548c \u201chealthy\u201d \u4e24\u7ec4\u6837\u672c\u7684\u5f97\u5206\uff1a<\/span><\/p>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">group=read.delim(<span class=\"code-snippet__string\">\"group.txt\"<\/span>,header=T,stringsAsFactors = FALSE,sep=<span class=\"code-snippet__string\">\"t\"<\/span>) <\/span><\/code><code><span class=\"code-snippet_outer\"> score=read.csv(<span class=\"code-snippet__string\">\"xCell_score.csv\"<\/span>,header=T,row.names=<span class=\"code-snippet__number\">1<\/span>,stringsAsFactors = FALSE)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u53d6\u51fa\u7ed3\u679c\u6587\u4ef6\u4e2d\u7684\u524d<span class=\"code-snippet__number\">64<\/span>\u4e2a\u6837\u672c\uff0c\u5e76\u5206\u522b\u53d6\u51fa\u201cseptic_shock\u201d\uff0c\u5373\u5e26\u6709\u75be\u75c5\u7684\u6837\u672c\u7fa4\u548c\u201chealthy\u201d\uff0c\u5373\u5065\u5eb7\u7684\u6837\u672c\u7fa4\u5230\u5bf9\u5e94\u7684\u6570\u636e\u96c6\u4e2d<\/span><\/code><code><span class=\"code-snippet_outer\"> case_data=score[<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">64<\/span>,group$group==<span class=\"code-snippet__string\">\"septic_shock\"<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\"> control_data=score[<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">64<\/span>,group$group==<span class=\"code-snippet__string\">\"healthy\"<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\"> all(row.names(case_data)==row.names(control_data))<\/span><\/code><code><span class=\"code-snippet_outer\"> normal=ncol(control_data)<\/span><\/code><code><span class=\"code-snippet_outer\"> tumor=ncol(case_data)<\/span><\/code><code><span class=\"code-snippet_outer\"> normal_data=as.data.frame(t(control_data))<\/span><\/code><code><span class=\"code-snippet_outer\"> tumor_data=as.data.frame(t(case_data))<\/span><\/code><code><span class=\"code-snippet_outer\"> #\u5408\u5e76\u4e24\u79cd\u7c7b\u578b\u7684\u7ed3\u679c\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\"> rt_total = rbind(normal_data,tumor_data)<\/span><\/code><code><span class=\"code-snippet_outer\"> cell_group_file=read.delim(<span class=\"code-snippet__string\">\"cell_type.txt\"<\/span>,header=T,stringsAsFactors = FALSE)<\/span><\/code><code><span class=\"code-snippet_outer\">cell_group=unique(cell_group_file$Subgroup)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"line-height: 0;text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><br \/><\/section>\n<\/section>\n<section style=\"line-height: 0;text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><br \/><\/section>\n<\/section>\n<section style=\"line-height: 0;text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><br \/><\/section>\n<\/section>\n<section style=\"line-height: 0;text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.4400871459694989\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_5b6n1ktWiauTdCD5ibDmDlYhn4Gvlg.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNylh9Ot54aFJuqnzRED27naywY85b6n1ktWiauTdCD5ibDmDlYhn4Gvlg\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"459\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section style=\"margin-top: 5px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<section>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;color: rgb(62, 62, 62);\">\u6574\u5408\u6837\u672c\u5206\u7ec4\u4fe1\u606f\u4e0e\u7ec6\u80de\u5206\u7ec4\u4fe1\u606f<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5904\u7406\u597d\u5206\u6790\u540e\u7684\u6570\u636e\u540e\uff0c\u6211\u4eec\u53ef\u4ee5\u901a\u8fc7\u8fdb\u4e00\u6b65\u6574\u5408\u7ed8\u5236\u6240\u6709\u7ed3\u679c\u6570\u636e\u96c6\u5bf9\u5e94\u7684\u70ed\u56fe\uff0c\u90a3\u5c31\u548c\u5c0f\u679c\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\u600e\u6837\u7ed8\u5236\u5427\uff01<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5728\u7ed8\u5236\u70ed\u56fe\u4e4b\u524d\uff0c\u6211\u4eec\u9996\u5148\u8981\u6574\u5408\u6570\u636e\u3001\u5bf9\u6240\u9700\u6570\u636e\u8fdb\u884c\u7b5b\u9009\u7b49\u64cd\u4f5c\uff0c\u4e3a\u540e\u7eed\u7684\u70ed\u56fe\u7ed8\u5236\u5de5\u4f5c\u505a\u597d\u51c6\u5907\u5de5\u4f5c\uff0c\u6211\u4eec\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\u5427\uff01<\/span><\/p>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">rt=t(rbind(normal_data,tumor_data)) #\u6b63\u5e38\u7ec4\u548c\u80bf\u7624\u7ec4\u7684\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u6309\u884c\u5408\u5e76\uff0c\u5e76\u8fdb\u884c\u8f6c\u7f6e\uff0c\u4f7f\u6837\u672c\u5728\u5217\u4e0a\u6392\u5217\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">cell_group_file=cell_group_file[order(cell_group_file$Subgroup),]<\/span><\/code><code><span class=\"code-snippet_outer\">rt=rt[cell_group_file$Cell.types,] #\u8bfb\u5165\u7ec6\u80de\u7c7b\u578b\u4fe1\u606f\u6587\u4ef6cell_group_file\uff0c\u5bf9\u5176\u6309\u7167Subgroup\u8fd9\u4e00\u5217\u5217\u8fdb\u884c\u6392\u5e8f<\/span><\/code><code><span class=\"code-snippet_outer\">all(row.names(rt)==cell_group_file$Cell.types)<\/span><\/code><code><span class=\"code-snippet_outer\">group1=c(rep(<span class=\"code-snippet__string\">\"healthy\"<\/span>,nrow(normal_data)), #\u5408\u5e76\u5065\u5eb7\u7ec4\u548c\u80bf\u7624\u7ec4\u6837\u672c<\/span><\/code><code><span class=\"code-snippet_outer\"> rep(<span class=\"code-snippet__string\">\"septic_shock\"<\/span>,nrow(tumor_data)))<\/span><\/code><code><span class=\"code-snippet_outer\">names(group1)=colnames(rt)<\/span><\/code><code><span class=\"code-snippet_outer\">group1=as.data.frame(group1)<\/span><\/code><code><span class=\"code-snippet_outer\">type=cell_group_file[,<span class=\"code-snippet__number\">2<\/span>:<span class=\"code-snippet__number\">3<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">row.names(type)=type$Cell.types<\/span><\/code><code><span class=\"code-snippet_outer\">type$Cell.types=<span class=\"code-snippet__literal\">NULL<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"line-height: 0;text-align: center;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.42061855670103093\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_M4YPbLrMhk5rkKib7KWjdWUhv6ZXoA.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNHMkkJngnf5Q03jSVl55kdgmnXM4YPbLrMhk5rkKib7KWjdWUhv6ZXoA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"485\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section style=\"margin-top: 5px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<section>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;color: rgb(62, 62, 62);\">\u751f\u6210\u70ed\u56fe<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u505a\u597d\u4e0a\u8ff0\u51c6\u5907\u5de5\u4f5c\u540e\uff0c\u6211\u4eec\u53ef\u4ee5\u5bfc\u5165pheatmap\u5305\u5e76\u7ed8\u5236\u70ed\u56fe\uff0c\u70ed\u56fe\u7684\u5177\u4f53\u7ed8\u5236\u65b9\u6cd5\u53ef\u4ee5\u770b\u5c0f\u679c\u4e4b\u524d\u53d1\u8fc7\u7684\u7b14\u8bb0\u54e6\uff0c\u63a5\u4e0b\u6765\u548c\u5c0f\u679c\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\u7ed8\u5236\u8fc7\u7a0b\u5427\uff01<\/span><\/p>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">library(pheatmap)<\/span><\/code><code><span class=\"code-snippet_outer\">pdf(<span class=\"code-snippet__string\">\"Heatmap.pdf\"<\/span>,height=<span class=\"code-snippet__number\">8<\/span>,width=<span class=\"code-snippet__number\">12<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> pheatmap(rt, <\/span><\/code><code><span class=\"code-snippet_outer\"> annotation_col =group1, <\/span><\/code><code><span class=\"code-snippet_outer\"> annotation_row=type,<\/span><\/code><code><span class=\"code-snippet_outer\"> annotation_colors =<span class=\"code-snippet__built_in\">list<\/span>(Type=c(healthy = <span class=\"code-snippet__string\">\"#42B540FF\"<\/span>, septic_shock = <span class=\"code-snippet__string\">\"#925E9FFF\"<\/span>)) ,<\/span><\/code><code><span class=\"code-snippet_outer\"> color = colorRampPalette(c(<span class=\"code-snippet__string\">\"green\"<\/span>, <span class=\"code-snippet__string\">\"black\"<\/span>, <span class=\"code-snippet__string\">\"red\"<\/span>))(<span class=\"code-snippet__number\">50<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> cluster_cols =F,<\/span><\/code><code><span class=\"code-snippet_outer\"> fontsize = <span class=\"code-snippet__number\">9<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> fontsize_row=<span class=\"code-snippet__number\">8<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> fontsize_col=<span class=\"code-snippet__number\">6<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> cluster_rows = F,<\/span><\/code><code><span class=\"code-snippet_outer\"> show_colnames = F)<\/span><\/code><code><span class=\"code-snippet_outer\">dev.off()<\/span><\/code><\/pre>\n<\/section>\n<section style=\"margin-top: 15px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<section>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u597d\u5566\uff01\u73b0\u5728\u4f60\u5df2\u7ecf\u6210\u529f\u5b8c\u6210\u4e86\u70ed\u56fe\u7684\u7ed8\u5236\uff0c\u63a5\u4e0b\u6765\u6211\u4eec\u770b\u770b\u7ed8\u5236\u6548\u679c\uff1a<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6666666666666666\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_F1Tvl0bicPHa4MruWAgyh1rniajdpQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNYvJ5zcL0KmhEpu4v31eBgXxmib9F1Tvl0bicPHa4MruWAgyh1rniajdpQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"567\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><br \/><\/p>\n<p style=\"white-space: normal;\"><em><strong><span style=\"font-size: 18px;\">xCell\u5bf9\u5206\u6790\u7ed3\u679c\u4e0b\u6e38\u5206\u6790<\/span><\/strong><\/em><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u540c\u6837\u5730\uff0c\u6211\u4eec\u4e5f\u53ef\u4ee5\u5bf9xCell\u5206\u6790\u7684\u7ed3\u679c\u8fdb\u884c\u4e0b\u6e38\u5206\u6790\u5e76\u901a\u8fc7\u5c0f\u63d0\u7434\u56fe\u5bf9\u7ed3\u679c\u8fdb\u884c\u76f4\u89c2\u7684\u5206\u6790\uff0c\u548c\u5c0f\u679c\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\u600e\u6837\u64cd\u4f5c\u5427\uff01<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u73b0\u5728\u6211\u4eec\u63d0\u53d6\u51fa\u4e86xCellAnalysis\u5206\u6790\u540e\u7684\u6837\u672c\u6570\u636e\u96c6\u5e76\u505a\u4e86\u5c01\u88c5\u5904\u7406\uff0c\u73b0\u5728\u6211\u4eec\u53ef\u4ee5\u9488\u5bf9\u6211\u4eec\u7684\u7ec6\u80de\u7fa4\u6570\u636e\u8fdb\u884c\u4e0b\u6e38\u5206\u6790\u4ee5\u53ca\u53ef\u89c6\u5316\u5904\u7406\u7684\u64cd\u4f5c\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4e3b\u8981\u7684\u6d41\u7a0b\u4e3a\uff1a\u6839\u636e\u4e0d\u540c\u7684\u7ec6\u80de\u5b50\u7fa4\u5bf9\u6837\u672c\u8fdb\u884c\u5206\u7c7b\uff0c\u904d\u5386\u6bcf\u4e2a\u7ec6\u80de\u5b50\u7fa4\uff0c\u5e76\u8ba1\u7b97\u6bcf\u4e2a\u5b50\u7fa4\u7684\u5dee\u5f02\u663e\u8457\u6027\uff0c\u5e76\u5bf9\u663e\u8457\u5dee\u5f02\u7684\u7ec6\u80de\u5b50\u7fa4\u8fdb\u884c\u7ed8\u56fe\u5c55\u793a\u548c\u4fdd\u5b58\u7ed3\u679c\u3002\u90a3\u4e48\u5c31\u8ba9\u6211\u4eec\u4e00\u8d77\u6765\u770b\u4e0b\u4ee3\u7801\u5b9e\u73b0\u5427\uff01<\/span><\/p>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span> (subgroup in cell_group){<\/span><\/code><code><span class=\"code-snippet_outer\"> s=which( cell_group_file$Subgroup==subgroup) #\u83b7\u53d6\u5f53\u524d\u7ec6\u80de\u6240\u5c5e\u5b50\u7fa4\u7684index<\/span><\/code><code><span class=\"code-snippet_outer\"> cells=cell_group_file$Cell.types[s] #\u63d0\u53d6\u5c5e\u4e8e\u5f53\u524d\u5b50\u7fa4\u7684\u6240\u6709\u7ec6\u80de<\/span><\/code><code><span class=\"code-snippet_outer\"> rt=rt_total[,cells] #\u63d0\u53d6\u5c5e\u4e8e\u5f53\u524d\u5b50\u7fa4\u7684\u6240\u6709\u7ec6\u80de\u7c7b\u578b\u7684\u6570\u636e\uff0c\u5e76\u4fdd\u5b58\u5728 rt \u4e2d<\/span><\/code><code><span class=\"code-snippet_outer\"> cell=c()<\/span><\/code><code><span class=\"code-snippet_outer\"> p.value=c()<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__keyword\">for<\/span>(i in <span class=\"code-snippet__number\">1<\/span>:ncol(rt)){<\/span><\/code><code><span class=\"code-snippet_outer\"> normalData=rt[<span class=\"code-snippet__number\">1<\/span>:normal,i]<\/span><\/code><code><span class=\"code-snippet_outer\"> tumorData=rt[(normal+<span class=\"code-snippet__number\">1<\/span>):(normal+tumor),i]<\/span><\/code><code><span class=\"code-snippet_outer\"> wilcoxtest=wilcox.test(normalData,tumorData,exact = F) #\u8ba1\u7b97\u5176\u5dee\u5f02\u7684 p-value\uff0c\u5e76\u4fdd\u5b58\u5728p.value\u4e2d\uff0c\u7ec6\u80de\u7c7b\u578b\u540d\u79f0\u4fdd\u5b58\u5728cell\u4e2d<\/span><\/code><code><span class=\"code-snippet_outer\"> p=round(wilcoxtest$p.value,<span class=\"code-snippet__number\">3<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> p.value=c(p.value,p) #\u5408\u5e76<\/span><\/code><code><span class=\"code-snippet_outer\"> cell=c(cell,colnames(rt)[i])<\/span><\/code><code><span class=\"code-snippet_outer\"> }<\/span><\/code><code><span class=\"code-snippet_outer\"> sig=data.frame(cell,p.value)<\/span><\/code><code><span class=\"code-snippet_outer\"> sig=sig[sig$p.value<<span class=\"code-snippet__number\">0.05<\/span>,] #\u9009\u51fap\u503c\u5c0f\u4e8e<span class=\"code-snippet__number\">0.05<\/span>\u7684\u663e\u8457\u7ec6\u80de\u7c7b\u578b<\/span><\/code><code><span class=\"code-snippet_outer\"> s=which(colnames(rt)%in%as.character(sig$cell))<\/span><\/code><code><span class=\"code-snippet_outer\"> rt=rt[,s]<\/span><\/code><code><span class=\"code-snippet_outer\"> all(colnames(rt)==as.character(sig$cell))<\/span><\/code><\/pre>\n<\/section>\n<section style=\"margin-top: 15px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<section>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u73b0\u5728\uff0c\u5c0f\u679c\u7ed9\u5927\u5bb6\u5c55\u793a\u904d\u5386\u5230\u7684\u5176\u4e2d\u4e00\u4e2a\u7ec6\u80de\u5b50\u7fa4\u7684\u6700\u7ec8\u8ba1\u7b97\u7ed3\u679c\u6570\u636e\uff0c\u5373\u6211\u4eec\u63d0\u53d6\u51fa\u7684\u8981\u53ef\u89c6\u5316\u7684\u6570\u636e\u96c6\uff0c\u4e00\u8d77\u6765\u770b\u770b\u5427\uff01<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9368231046931408\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_aRG2rEicEAAx2huhGzr5wvviarlktg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNCKLQtYTrHqDNcPeT3PEqz16sooaRG2rEicEAAx2huhGzr5wvviarlktg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><\/p>\n<p style=\"white-space: normal;\"><strong><em><span style=\"font-size: 18px;color: rgb(62, 62, 62);\">\u901a\u8fc7\u5c0f\u63d0\u7434\u56fe\u53ef\u89c6\u5316\u4e0b\u6e38\u5206\u6790\u7684\u7ed3\u679c<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u73b0\u5728\uff0c\u6211\u4eec\u53ef\u4ee5\u5c06\u6700\u7ec8\u7684\u8ba1\u7b97\u7ed3\u679c\u901a\u8fc7\u5c0f\u63d0\u7434\u56fe\u5c55\u793a\u51fa\u6765\uff0c\u8ba9\u6211\u4eec\u6765\u770b\u770b\u5177\u4f53\u7684\u4ee3\u7801\u5b9e\u73b0\u5427\uff01<\/span><\/p>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">library(vioplot)<\/span><\/code><code><span class=\"code-snippet_outer\">pdf(paste(subgroup,<span class=\"code-snippet__string\">\"Xcell_score.pdf\"<\/span>,sep=<span class=\"code-snippet__string\">\"_\"<\/span>),height=<span class=\"code-snippet__number\">8<\/span>,width=<span class=\"code-snippet__number\">15<\/span>) #\u8bbe\u7f6e\u53ef\u89c6\u5316\u548c\u4fdd\u5b58\u7684\u6587\u4ef6\u540d\u79f0<\/span><\/code><code><span class=\"code-snippet_outer\">par(las=<span class=\"code-snippet__number\">1<\/span>,mar=c(<span class=\"code-snippet__number\">10<\/span>,<span class=\"code-snippet__number\">6<\/span>,<span class=\"code-snippet__number\">3<\/span>,<span class=\"code-snippet__number\">3<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\">x=c(<span class=\"code-snippet__number\">1<\/span>:ncol(rt))<\/span><\/code><code><span class=\"code-snippet_outer\">y=c(<span class=\"code-snippet__number\">1<\/span>:ncol(rt))<\/span><\/code><code><span class=\"code-snippet_outer\">#\u7ed8\u5236\u7a7a\u767d\u6563\u70b9\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\">plot(x,y,<\/span><\/code><code><span class=\"code-snippet_outer\"> xlim=c(<span class=\"code-snippet__number\">0<\/span>,(ncol(rt)<span class=\"code-snippet__number\">-1<\/span>)*<span class=\"code-snippet__number\">3<\/span>+<span class=\"code-snippet__number\">2<\/span>),ylim=c(min(rt),max(rt)+<span class=\"code-snippet__number\">0.02<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> main=<span class=\"code-snippet__string\">\"GSE57065\"<\/span>,xlab=<span class=\"code-snippet__string\">\"\"<\/span>, ylab=<span class=\"code-snippet__string\">\"Xcell score\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> pch=<span class=\"code-snippet__number\">21<\/span>,col=<span class=\"code-snippet__string\">\"white\"<\/span>,xaxt=<span class=\"code-snippet__string\">\"n\"<\/span>,cex.lab=<span class=\"code-snippet__number\">1.3<\/span>,cex.main=<span class=\"code-snippet__number\">1.5<\/span>) <\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__keyword\">for<\/span>(i in <span class=\"code-snippet__number\">1<\/span>:ncol(rt)){<\/span><\/code><code><span class=\"code-snippet_outer\"> normalData=rt[<span class=\"code-snippet__number\">1<\/span>:normal,i]<\/span><\/code><code><span class=\"code-snippet_outer\"> tumorData=rt[(normal+<span class=\"code-snippet__number\">1<\/span>):(normal+tumor),i]<\/span><\/code><code><span class=\"code-snippet_outer\"> wilcoxtest=wilcox.test(normalData,tumorData,exact = F)<\/span><\/code><code><span class=\"code-snippet_outer\"> p=round(wilcoxtest$p.value,<span class=\"code-snippet__number\">3<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> vioplot(normalData,at=<span class=\"code-snippet__number\">3<\/span>*(i<span class=\"code-snippet__number\">-1<\/span>),lty=<span class=\"code-snippet__number\">1<\/span>,add = T,col = <span class=\"code-snippet__string\">'#42B540FF'<\/span>) #\u7ed8\u5236\u6b63\u5e38\u6570\u636e\u7684\u5c0f\u63d0\u7434\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"> vioplot(tumorData,at=<span class=\"code-snippet__number\">3<\/span>*(i<span class=\"code-snippet__number\">-1<\/span>)+<span class=\"code-snippet__number\">1<\/span>,lty=<span class=\"code-snippet__number\">1<\/span>,add = T,col = <span class=\"code-snippet__string\">'#925E9FFF'<\/span>) #\u7ed8\u5236\u80bf\u7624\u6570\u636e\u7684\u5c0f\u63d0\u7434\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"> mx=max(c(normalData,tumorData))<\/span><\/code><code><span class=\"code-snippet_outer\"> lines(c(x=<span class=\"code-snippet__number\">3<\/span>*(i<span class=\"code-snippet__number\">-1<\/span>)+<span class=\"code-snippet__number\">0.2<\/span>,x=<span class=\"code-snippet__number\">3<\/span>*(i<span class=\"code-snippet__number\">-1<\/span>)+<span class=\"code-snippet__number\">0.8<\/span>),c(mx,mx))<\/span><\/code><code><span class=\"code-snippet_outer\"> text(x=<span class=\"code-snippet__number\">3<\/span>*(i<span class=\"code-snippet__number\">-1<\/span>)+<span class=\"code-snippet__number\">1<\/span>,y=mx+<span class=\"code-snippet__number\">0.02<\/span>,labels=ifelse(p<<span class=\"code-snippet__number\">0.001<\/span>,paste0(<span class=\"code-snippet__string\">\"p<0.001\"<\/span>),paste0(<span class=\"code-snippet__string\">\"p=\"<\/span>,p)),cex = <span class=\"code-snippet__number\">0.8<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> }<\/span><\/code><code><span class=\"code-snippet_outer\"> text(seq(<span class=\"code-snippet__number\">1<\/span>,((ncol(rt)<span class=\"code-snippet__number\">-1<\/span>)*<span class=\"code-snippet__number\">3<\/span>+<span class=\"code-snippet__number\">1<\/span>),<span class=\"code-snippet__number\">3<\/span>),<span class=\"code-snippet__number\">-0.01<\/span>,xpd = NA,labels=colnames(rt),cex = <span class=\"code-snippet__number\">1<\/span>,srt = <span class=\"code-snippet__number\">45<\/span>,pos=<span class=\"code-snippet__number\">2<\/span>,font=<span class=\"code-snippet__number\">2<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> dev.off()<\/span><\/code><code><span class=\"code-snippet_outer\"> write.csv(sig,paste(subgroup,<span class=\"code-snippet__string\">\"_sig_cell.csv\"<\/span>,sep=<span class=\"code-snippet__string\">\"\"<\/span>)) #\u4fdd\u5b58\u6700\u7ec8\u7ed3\u679c <\/span><\/code><code><span class=\"code-snippet_outer\">} <\/span><\/code><\/pre>\n<\/section>\n<section style=\"margin-top: 15px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<section>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u73b0\u5728\uff0c\u4f60\u5df2\u7ecf\u6210\u529f\u5b8c\u6210\u4e86xCell\u5206\u6790\u7ec6\u80de\u5230\u53ef\u89c6\u5316\u7684\u5de6\u53f3\u5de5\u4f5c\uff0c\u73b0\u5728\u5c0f\u679c\u7ed9\u5927\u5bb6\u5c55\u793a\u5176\u4e2d\u4e00\u4e2a\u7ec6\u80de\u5b50\u7fa4\u7684\u4e0b\u6e38\u5206\u6790\u53ef\u89c6\u5316\u7ed3\u679c\uff01\u4e00\u8d77\u6765\u770b\u5427\uff01<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.4828767123287671\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_IFHHvkuYvorKoAibEv7ickxRdKALzg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNxTtxS8QqsGGfAicthiasv9ia3jxdyIFHHvkuYvorKoAibEv7ickxRdKALzg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"584\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u600e\u4e48\u6837\uff0c\u4f60\u5b66\u4f1a\u600e\u4e48\u4f7f\u7528xCell\u5305\u4e86\u561b? \u66f4\u591a\u5b66\u4e60\u8d44\u6e90\u8bf7\u5927\u5bb6\u79fb\u6b65\u5c0f\u679c\u4e13\u5c5e\u4e91\u751f\u4fe1\u5e73\u53f0\u641c\u7d22\u66f4\u591a\u8d44\u6e90\u54e6\uff01<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\"><span style=\"font-size: 14px;letter-spacing: 0.578px;\">\u5c0f\u679c\u4e13\u5c5e\u4e91\u751f\u4fe1\u5e73\u53f0<\/span>\uff1a<\/span><em style=\"letter-spacing: 0.578px;color: rgb(0, 0, 0);font-size: 17px;line-height: 1.59em;\"><strong>\u4e91\u751f\u4fe1 – \u5b66\u751f\u7269\u4fe1\u606f\u5b66 (biocloudservice.com)<\/strong><\/em><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\"><span style=\"font-size: 14px;letter-spacing: 0.578px;\">\u4e91\u751f\u4fe1\u5e73\u53f0\u4e5f\u6709\u514d\u75ab\u4e13\u7248\u7684\u5b66\u4e60\u6a21\u5757\u54e6\uff0c\u5feb\u6765\u627e\u5230\u4f60\u60f3\u5b66\u4e60\u7684\u4e13\u5c5e\u6a21\u5757\u5427\uff01<\/span>\u5982\u679c\u60f3\u7528\u670d\u52a1\u5668\u53ef\u4ee5\u8054\u7cfb\u5fae\u4fe1\uff1a<\/span><em style=\"letter-spacing: 0.578px;color: rgb(0, 0, 0);font-size: 17px;line-height: 1.59em;\"><strong>18502195490\uff08\u5feb\u6765\u8054\u7cfb\u6211\u4eec\u4f7f\u7528\u5427\uff01\uff09<\/strong><\/em><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section data-tools=\"135\u7f16\u8f91\u5668\" data-id=\"105054\" style=\"font-size: 16px;\">\n<section style=\"margin: 10px auto;text-align: center;\">\n<section style=\"padding-top: 30px;padding-bottom: 30px;background-color: rgb(242, 246, 255);border-radius: 7px;\">\n<section style=\"display: flex;justify-content: space-evenly;align-items: center;\">\n<section 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class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-05-16 19:01:06<\/em><\/div>\n<p><content><\/p>\n<section data-role=\"outer\" label=\"edit by 135editor\">\n<section style=\"letter-spacing: 0.578px;font-size: 16px;\">\n<section>\n<section>\n<section>\n<section>\n<section>\n<section data-width=\"100%\" style=\"width: 100%;flex: 0 0 100%;opacity: 1;\">\n<section style=\"line-height: 1.8;\">\n<section>\n<section>\n<section style=\"margin-top: 10px;margin-bottom: 20px;display: flex;flex-flow: row nowrap;text-align: center;justify-content: center;\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;border-style: solid;border-width: 1px;border-color: rgb(67, 67, 67);flex: 0 0 auto;align-self: flex-start;height: auto;box-shadow: rgb(255, 219, 118) 6px 6px 0px;background-color: rgb(255, 255, 255);\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 70%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 70% !important;\">\n<section style=\"margin-top: 10px;\">\n<section style=\"text-align: center;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u751f\u4fe1\u4ebaR\u8bed\u8a00\u5b66\u4e60\u5fc5\u5907<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u7acb\u523b\u62e5\u6709\u4e00\u4e2aRstudio\u8d26\u53f7<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u5f00\u542f\u5347\u7ea7\u6a21\u5f0f\u5427<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"color: rgb(255, 129, 36);font-size: 12px;letter-spacing: 0.5px;\">\uff0856\u7ebf\u7a0b\uff0c256G\u5185\u5b58\uff0c\u4e2a\u4eba\u5b58\u50a81T\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;width: 30%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 30% !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"162\" data-cropsely1=\"0\" data-cropsely2=\"162\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_ib2l1ye6HNIkwTG696C2pf9yAtdeGA-25.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAyB69UyXDLG0wQcISNwmL6DlpIKVBnCSOXHTWJhLcxic2qtibaib2l1ye6HNIkwTG696C2pf9yAtdeGA\/640?wx_fmt=png'\" data-type=\"jpeg\" style=\"height: 111px;vertical-align: middle;width: 111px;\" data-ratio=\"1\" data-w=\"400\" data-recalc-dims=\"1\" \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section data-role=\"list\">\n<ul class=\"list-paddingleft-1\" style=\"padding-left: 30px;width: 100%;list-style-position: outside;\">\n<li>\n<section><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">hi\u5927\u5bb6\uff0c\u597d\u4e45\u4e0d\u89c1\uff0c\u5c0f\u679c\u53c8\u6765\u5566\uff01<\/span><\/section>\n<\/li>\n<li>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\">xCell\u662f\u4e00\u79cd\u57fa\u4e8e\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u7684\u514d\u75ab\u7ec6\u80de\u6d78\u6da6\u8bc4\u4f30\u5de5\u5177\uff0c\u53ef\u4ee5\u8bc6\u522b\u51fa\u6f5c\u5728\u7684\u514d\u75ab\u7ec6\u80de\u4e9a\u7fa4\u5e76\u8ba1\u7b97\u5b83\u4eec\u5728\u7ec4\u7ec7\u4e2d\u7684\u76f8\u5bf9\u4e30\u5ea6\u3002\u5b83\u53ef\u4ee5\u5e7f\u6cdb\u5e94\u7528\u4e8e\u764c\u75c7\u3001\u81ea\u8eab\u514d\u75ab\u6027\u75be\u75c5\u548c\u5176\u4ed6\u75be\u75c5\u7684\u514d\u75ab\u6d78\u6da6\u5206\u6790\u4e2d\u3002<\/span><\/p>\n<\/li>\n<\/ul>\n<section>\n<section>\n<section><br \/><\/section>\n<section style=\"font-size: 16px;\">\n<section style=\"margin-top: 5px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4eca\u5929\u5c0f\u679c\u5c31\u6765\u6559\u5927\u5bb6\u5982\u4f55\u5b9e\u6218\u5e94\u7528xCell\u8fdb\u884c\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u7684\u514d\u75ab\u6d78\u6da6\u5206\u6790\uff0c\u90a3\u5c31\u548c\u5c0f\u679c\u4e00\u8d77\u6765\u770b\u4e0b\u5427\uff01<\/span><\/p>\n<\/section>\n<section style=\"line-height: 0;text-align: center;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_QiaOCcR0jFpvjkSAu3UmKL82ohCV5Q-1.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNXhQ4GD7nqK0jxIA9vNfaZMfcibQiaOCcR0jFpvjkSAu3UmKL82ohCV5Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"500\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section style=\"margin-top: 5px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<section>\n<p style=\"text-align: center;white-space: normal;\"><em><strong><span style=\"font-size: 20px;\">\u6570\u636e\u9884\u5904\u7406<\/span><\/strong><\/em><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4e0b\u8f7dxCell\u5305\u5e76\u5bfc\u5165\uff1a<\/span><\/p>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">'devtools'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">devtools::install_github(<span class=\"code-snippet__string\">'dviraran\/xCell'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">library(xCell)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"line-height: 0;text-align: center;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_s2ZHCtbN9nDKgpfniawYJJzqxT6v6g.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNq0nuSrPNnVriasrjcop8ONDW3ss2ZHCtbN9nDKgpfniawYJJzqxT6v6g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"500\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section style=\"margin-top: 5px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<section>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;color: rgb(62, 62, 62);\">xCell\u514d\u75ab\u6d78\u6da6\u5206\u6790<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5bfc\u5165\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\uff0c\u5e76\u901a\u8fc7xCellAnalysis\u5206\u6790\u8868\u8fbe\u77e9\u9635\uff0c\u8be5\u51fd\u6570\u7528\u4e8e\u9884\u6d4b\u7ec6\u80de\u7ec4\u5206\u5728\u7ed9\u5b9a\u6837\u672c\u4e2d\u7684\u76f8\u5bf9\u4e30\u5ea6\uff1a<\/span><\/p>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">exprSet=read.csv(<span class=\"code-snippet__string\">\"GSE57065_ExprMatrix.csv\"<\/span>,header=T,row.names=<span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">xCell <- xCellAnalysis(exprSet) ##<span class=\"code-snippet__meta\">#array data<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">write.csv(xCell,<span class=\"code-snippet__string\">\"xCell_score.csv\"<\/span>) #\u5c06\u8ba1\u7b97\u540e\u7684\u7ed3\u679c\u5199\u5165\u8868\u683c\u6587\u4ef6\u4e2d<\/span><\/code><\/pre>\n<\/section>\n<section style=\"margin-top: 5px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5148\u6765\u4e00\u8d77\u770b\u4e0b\u5206\u6790\u540e\u7684\u6570\u636e\u96c6\u5427\uff01<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6371841155234657\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_fUBIIH3vsVekJbmH1KnO48DII995DA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNcJeIyg3dEFnePCe7O2ib4bGcUfUBIIH3vsVekJbmH1KnO48DII995DA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;text-align: center;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.49899799599198397\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_PSgMgn9cx3xIMRpoK7HUMUxLr0HL3A.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNszG7picpYriceh3kdysIicAjyUtPSgMgn9cx3xIMRpoK7HUMUxLr0HL3A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"499\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section style=\"margin-top: 5px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<section>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;color: rgb(62, 62, 62);\">\u5904\u7406xCell\u5206\u6790\u540e\u7684\u7ed3\u679c\u6570\u636e\u96c6<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u53ef\u4ee5\u6839\u636e\u6837\u672c\u7684\u5206\u7ec4\u60c5\u51b5\uff0c\u63d0\u53d6\u51fa\u5bf9\u5e94\u7684\u7ec6\u80de\u7c7b\u578b\u5f97\u5206\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u9996\u5148\u6211\u4eec\u8981\u8bfb\u53d6 group.txt \u7684\u6587\u4ef6\uff0c\u8fd9\u91cc\u5305\u542b\u4e86\u6837\u672c\u7684\u5206\u7ec4\u4fe1\u606f\uff0c\u6e29\u99a8\u63d0\u793a\uff0c\u7ec6\u80de\u8868\u8fbe\u6837\u672c\u6570\u636e\u5305\u542b\u4e24\u79cd\u7c7b\u578b\u54e6\uff01<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u6211\u4eec\u53ef\u4ee5\u8bfb\u5165\u8fd9\u4e2a\u6587\u4ef6\uff0c\u5e76\u63d0\u53d6\u51fa\u201cseptic_shock\u201d \u548c \u201chealthy\u201d \u4e24\u7ec4\u6837\u672c\u7684\u5f97\u5206\uff1a<\/span><\/p>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">group=read.delim(<span class=\"code-snippet__string\">\"group.txt\"<\/span>,header=T,stringsAsFactors = FALSE,sep=<span class=\"code-snippet__string\">\"t\"<\/span>) <\/span><\/code><code><span class=\"code-snippet_outer\"> score=read.csv(<span class=\"code-snippet__string\">\"xCell_score.csv\"<\/span>,header=T,row.names=<span class=\"code-snippet__number\">1<\/span>,stringsAsFactors = FALSE)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u53d6\u51fa\u7ed3\u679c\u6587\u4ef6\u4e2d\u7684\u524d<span class=\"code-snippet__number\">64<\/span>\u4e2a\u6837\u672c\uff0c\u5e76\u5206\u522b\u53d6\u51fa\u201cseptic_shock\u201d\uff0c\u5373\u5e26\u6709\u75be\u75c5\u7684\u6837\u672c\u7fa4\u548c\u201chealthy\u201d\uff0c\u5373\u5065\u5eb7\u7684\u6837\u672c\u7fa4\u5230\u5bf9\u5e94\u7684\u6570\u636e\u96c6\u4e2d<\/span><\/code><code><span class=\"code-snippet_outer\"> case_data=score[<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">64<\/span>,group$group==<span class=\"code-snippet__string\">\"septic_shock\"<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\"> control_data=score[<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">64<\/span>,group$group==<span class=\"code-snippet__string\">\"healthy\"<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\"> all(row.names(case_data)==row.names(control_data))<\/span><\/code><code><span class=\"code-snippet_outer\"> normal=ncol(control_data)<\/span><\/code><code><span class=\"code-snippet_outer\"> tumor=ncol(case_data)<\/span><\/code><code><span class=\"code-snippet_outer\"> normal_data=as.data.frame(t(control_data))<\/span><\/code><code><span class=\"code-snippet_outer\"> tumor_data=as.data.frame(t(case_data))<\/span><\/code><code><span class=\"code-snippet_outer\"> #\u5408\u5e76\u4e24\u79cd\u7c7b\u578b\u7684\u7ed3\u679c\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\"> rt_total = rbind(normal_data,tumor_data)<\/span><\/code><code><span class=\"code-snippet_outer\"> cell_group_file=read.delim(<span class=\"code-snippet__string\">\"cell_type.txt\"<\/span>,header=T,stringsAsFactors = FALSE)<\/span><\/code><code><span class=\"code-snippet_outer\">cell_group=unique(cell_group_file$Subgroup)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"line-height: 0;text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><br \/><\/section>\n<\/section>\n<section style=\"line-height: 0;text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><br \/><\/section>\n<\/section>\n<section style=\"line-height: 0;text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><br \/><\/section>\n<\/section>\n<section style=\"line-height: 0;text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.4400871459694989\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_5b6n1ktWiauTdCD5ibDmDlYhn4Gvlg.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNylh9Ot54aFJuqnzRED27naywY85b6n1ktWiauTdCD5ibDmDlYhn4Gvlg\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"459\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section style=\"margin-top: 5px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<section>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;color: rgb(62, 62, 62);\">\u6574\u5408\u6837\u672c\u5206\u7ec4\u4fe1\u606f\u4e0e\u7ec6\u80de\u5206\u7ec4\u4fe1\u606f<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5904\u7406\u597d\u5206\u6790\u540e\u7684\u6570\u636e\u540e\uff0c\u6211\u4eec\u53ef\u4ee5\u901a\u8fc7\u8fdb\u4e00\u6b65\u6574\u5408\u7ed8\u5236\u6240\u6709\u7ed3\u679c\u6570\u636e\u96c6\u5bf9\u5e94\u7684\u70ed\u56fe\uff0c\u90a3\u5c31\u548c\u5c0f\u679c\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\u600e\u6837\u7ed8\u5236\u5427\uff01<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5728\u7ed8\u5236\u70ed\u56fe\u4e4b\u524d\uff0c\u6211\u4eec\u9996\u5148\u8981\u6574\u5408\u6570\u636e\u3001\u5bf9\u6240\u9700\u6570\u636e\u8fdb\u884c\u7b5b\u9009\u7b49\u64cd\u4f5c\uff0c\u4e3a\u540e\u7eed\u7684\u70ed\u56fe\u7ed8\u5236\u5de5\u4f5c\u505a\u597d\u51c6\u5907\u5de5\u4f5c\uff0c\u6211\u4eec\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\u5427\uff01<\/span><\/p>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">rt=t(rbind(normal_data,tumor_data)) #\u6b63\u5e38\u7ec4\u548c\u80bf\u7624\u7ec4\u7684\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u6309\u884c\u5408\u5e76\uff0c\u5e76\u8fdb\u884c\u8f6c\u7f6e\uff0c\u4f7f\u6837\u672c\u5728\u5217\u4e0a\u6392\u5217\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">cell_group_file=cell_group_file[order(cell_group_file$Subgroup),]<\/span><\/code><code><span class=\"code-snippet_outer\">rt=rt[cell_group_file$Cell.types,] #\u8bfb\u5165\u7ec6\u80de\u7c7b\u578b\u4fe1\u606f\u6587\u4ef6cell_group_file\uff0c\u5bf9\u5176\u6309\u7167Subgroup\u8fd9\u4e00\u5217\u5217\u8fdb\u884c\u6392\u5e8f<\/span><\/code><code><span class=\"code-snippet_outer\">all(row.names(rt)==cell_group_file$Cell.types)<\/span><\/code><code><span class=\"code-snippet_outer\">group1=c(rep(<span class=\"code-snippet__string\">\"healthy\"<\/span>,nrow(normal_data)), #\u5408\u5e76\u5065\u5eb7\u7ec4\u548c\u80bf\u7624\u7ec4\u6837\u672c<\/span><\/code><code><span class=\"code-snippet_outer\"> rep(<span class=\"code-snippet__string\">\"septic_shock\"<\/span>,nrow(tumor_data)))<\/span><\/code><code><span class=\"code-snippet_outer\">names(group1)=colnames(rt)<\/span><\/code><code><span class=\"code-snippet_outer\">group1=as.data.frame(group1)<\/span><\/code><code><span class=\"code-snippet_outer\">type=cell_group_file[,<span class=\"code-snippet__number\">2<\/span>:<span class=\"code-snippet__number\">3<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">row.names(type)=type$Cell.types<\/span><\/code><code><span class=\"code-snippet_outer\">type$Cell.types=<span class=\"code-snippet__literal\">NULL<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"line-height: 0;text-align: center;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.42061855670103093\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_M4YPbLrMhk5rkKib7KWjdWUhv6ZXoA.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNHMkkJngnf5Q03jSVl55kdgmnXM4YPbLrMhk5rkKib7KWjdWUhv6ZXoA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"485\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section style=\"margin-top: 5px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<section>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;color: rgb(62, 62, 62);\">\u751f\u6210\u70ed\u56fe<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u505a\u597d\u4e0a\u8ff0\u51c6\u5907\u5de5\u4f5c\u540e\uff0c\u6211\u4eec\u53ef\u4ee5\u5bfc\u5165pheatmap\u5305\u5e76\u7ed8\u5236\u70ed\u56fe\uff0c\u70ed\u56fe\u7684\u5177\u4f53\u7ed8\u5236\u65b9\u6cd5\u53ef\u4ee5\u770b\u5c0f\u679c\u4e4b\u524d\u53d1\u8fc7\u7684\u7b14\u8bb0\u54e6\uff0c\u63a5\u4e0b\u6765\u548c\u5c0f\u679c\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\u7ed8\u5236\u8fc7\u7a0b\u5427\uff01<\/span><\/p>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">library(pheatmap)<\/span><\/code><code><span class=\"code-snippet_outer\">pdf(<span class=\"code-snippet__string\">\"Heatmap.pdf\"<\/span>,height=<span class=\"code-snippet__number\">8<\/span>,width=<span class=\"code-snippet__number\">12<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> pheatmap(rt, <\/span><\/code><code><span class=\"code-snippet_outer\"> annotation_col =group1, <\/span><\/code><code><span class=\"code-snippet_outer\"> annotation_row=type,<\/span><\/code><code><span class=\"code-snippet_outer\"> annotation_colors =<span class=\"code-snippet__built_in\">list<\/span>(Type=c(healthy = <span class=\"code-snippet__string\">\"#42B540FF\"<\/span>, septic_shock = <span class=\"code-snippet__string\">\"#925E9FFF\"<\/span>)) ,<\/span><\/code><code><span class=\"code-snippet_outer\"> color = colorRampPalette(c(<span class=\"code-snippet__string\">\"green\"<\/span>, <span class=\"code-snippet__string\">\"black\"<\/span>, <span class=\"code-snippet__string\">\"red\"<\/span>))(<span class=\"code-snippet__number\">50<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> cluster_cols =F,<\/span><\/code><code><span class=\"code-snippet_outer\"> fontsize = <span class=\"code-snippet__number\">9<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> fontsize_row=<span class=\"code-snippet__number\">8<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> fontsize_col=<span class=\"code-snippet__number\">6<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> cluster_rows = F,<\/span><\/code><code><span class=\"code-snippet_outer\"> show_colnames = F)<\/span><\/code><code><span class=\"code-snippet_outer\">dev.off()<\/span><\/code><\/pre>\n<\/section>\n<section style=\"margin-top: 15px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<section>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u597d\u5566\uff01\u73b0\u5728\u4f60\u5df2\u7ecf\u6210\u529f\u5b8c\u6210\u4e86\u70ed\u56fe\u7684\u7ed8\u5236\uff0c\u63a5\u4e0b\u6765\u6211\u4eec\u770b\u770b\u7ed8\u5236\u6548\u679c\uff1a<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6666666666666666\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_F1Tvl0bicPHa4MruWAgyh1rniajdpQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNYvJ5zcL0KmhEpu4v31eBgXxmib9F1Tvl0bicPHa4MruWAgyh1rniajdpQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"567\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><br \/><\/p>\n<p style=\"white-space: normal;\"><em><strong><span style=\"font-size: 18px;\">xCell\u5bf9\u5206\u6790\u7ed3\u679c\u4e0b\u6e38\u5206\u6790<\/span><\/strong><\/em><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u540c\u6837\u5730\uff0c\u6211\u4eec\u4e5f\u53ef\u4ee5\u5bf9xCell\u5206\u6790\u7684\u7ed3\u679c\u8fdb\u884c\u4e0b\u6e38\u5206\u6790\u5e76\u901a\u8fc7\u5c0f\u63d0\u7434\u56fe\u5bf9\u7ed3\u679c\u8fdb\u884c\u76f4\u89c2\u7684\u5206\u6790\uff0c\u548c\u5c0f\u679c\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\u600e\u6837\u64cd\u4f5c\u5427\uff01<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u73b0\u5728\u6211\u4eec\u63d0\u53d6\u51fa\u4e86xCellAnalysis\u5206\u6790\u540e\u7684\u6837\u672c\u6570\u636e\u96c6\u5e76\u505a\u4e86\u5c01\u88c5\u5904\u7406\uff0c\u73b0\u5728\u6211\u4eec\u53ef\u4ee5\u9488\u5bf9\u6211\u4eec\u7684\u7ec6\u80de\u7fa4\u6570\u636e\u8fdb\u884c\u4e0b\u6e38\u5206\u6790\u4ee5\u53ca\u53ef\u89c6\u5316\u5904\u7406\u7684\u64cd\u4f5c\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4e3b\u8981\u7684\u6d41\u7a0b\u4e3a\uff1a\u6839\u636e\u4e0d\u540c\u7684\u7ec6\u80de\u5b50\u7fa4\u5bf9\u6837\u672c\u8fdb\u884c\u5206\u7c7b\uff0c\u904d\u5386\u6bcf\u4e2a\u7ec6\u80de\u5b50\u7fa4\uff0c\u5e76\u8ba1\u7b97\u6bcf\u4e2a\u5b50\u7fa4\u7684\u5dee\u5f02\u663e\u8457\u6027\uff0c\u5e76\u5bf9\u663e\u8457\u5dee\u5f02\u7684\u7ec6\u80de\u5b50\u7fa4\u8fdb\u884c\u7ed8\u56fe\u5c55\u793a\u548c\u4fdd\u5b58\u7ed3\u679c\u3002\u90a3\u4e48\u5c31\u8ba9\u6211\u4eec\u4e00\u8d77\u6765\u770b\u4e0b\u4ee3\u7801\u5b9e\u73b0\u5427\uff01<\/span><\/p>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span> (subgroup in cell_group){<\/span><\/code><code><span class=\"code-snippet_outer\"> s=which( cell_group_file$Subgroup==subgroup) #\u83b7\u53d6\u5f53\u524d\u7ec6\u80de\u6240\u5c5e\u5b50\u7fa4\u7684index<\/span><\/code><code><span class=\"code-snippet_outer\"> cells=cell_group_file$Cell.types[s] #\u63d0\u53d6\u5c5e\u4e8e\u5f53\u524d\u5b50\u7fa4\u7684\u6240\u6709\u7ec6\u80de<\/span><\/code><code><span class=\"code-snippet_outer\"> rt=rt_total[,cells] #\u63d0\u53d6\u5c5e\u4e8e\u5f53\u524d\u5b50\u7fa4\u7684\u6240\u6709\u7ec6\u80de\u7c7b\u578b\u7684\u6570\u636e\uff0c\u5e76\u4fdd\u5b58\u5728 rt \u4e2d<\/span><\/code><code><span class=\"code-snippet_outer\"> cell=c()<\/span><\/code><code><span class=\"code-snippet_outer\"> p.value=c()<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__keyword\">for<\/span>(i in <span class=\"code-snippet__number\">1<\/span>:ncol(rt)){<\/span><\/code><code><span class=\"code-snippet_outer\"> normalData=rt[<span class=\"code-snippet__number\">1<\/span>:normal,i]<\/span><\/code><code><span class=\"code-snippet_outer\"> tumorData=rt[(normal+<span class=\"code-snippet__number\">1<\/span>):(normal+tumor),i]<\/span><\/code><code><span class=\"code-snippet_outer\"> wilcoxtest=wilcox.test(normalData,tumorData,exact = F) #\u8ba1\u7b97\u5176\u5dee\u5f02\u7684 p-value\uff0c\u5e76\u4fdd\u5b58\u5728p.value\u4e2d\uff0c\u7ec6\u80de\u7c7b\u578b\u540d\u79f0\u4fdd\u5b58\u5728cell\u4e2d<\/span><\/code><code><span class=\"code-snippet_outer\"> p=round(wilcoxtest$p.value,<span class=\"code-snippet__number\">3<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> p.value=c(p.value,p) #\u5408\u5e76<\/span><\/code><code><span class=\"code-snippet_outer\"> cell=c(cell,colnames(rt)[i])<\/span><\/code><code><span class=\"code-snippet_outer\"> }<\/span><\/code><code><span class=\"code-snippet_outer\"> sig=data.frame(cell,p.value)<\/span><\/code><code><span class=\"code-snippet_outer\"> sig=sig[sig$p.value<<span class=\"code-snippet__number\">0.05<\/span>,] #\u9009\u51fap\u503c\u5c0f\u4e8e<span class=\"code-snippet__number\">0.05<\/span>\u7684\u663e\u8457\u7ec6\u80de\u7c7b\u578b<\/span><\/code><code><span class=\"code-snippet_outer\"> s=which(colnames(rt)%in%as.character(sig$cell))<\/span><\/code><code><span class=\"code-snippet_outer\"> rt=rt[,s]<\/span><\/code><code><span class=\"code-snippet_outer\"> all(colnames(rt)==as.character(sig$cell))<\/span><\/code><\/pre>\n<\/section>\n<section style=\"margin-top: 15px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<section>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u73b0\u5728\uff0c\u5c0f\u679c\u7ed9\u5927\u5bb6\u5c55\u793a\u904d\u5386\u5230\u7684\u5176\u4e2d\u4e00\u4e2a\u7ec6\u80de\u5b50\u7fa4\u7684\u6700\u7ec8\u8ba1\u7b97\u7ed3\u679c\u6570\u636e\uff0c\u5373\u6211\u4eec\u63d0\u53d6\u51fa\u7684\u8981\u53ef\u89c6\u5316\u7684\u6570\u636e\u96c6\uff0c\u4e00\u8d77\u6765\u770b\u770b\u5427\uff01<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9368231046931408\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_aRG2rEicEAAx2huhGzr5wvviarlktg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNCKLQtYTrHqDNcPeT3PEqz16sooaRG2rEicEAAx2huhGzr5wvviarlktg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><\/p>\n<p style=\"white-space: normal;\"><strong><em><span style=\"font-size: 18px;color: rgb(62, 62, 62);\">\u901a\u8fc7\u5c0f\u63d0\u7434\u56fe\u53ef\u89c6\u5316\u4e0b\u6e38\u5206\u6790\u7684\u7ed3\u679c<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u73b0\u5728\uff0c\u6211\u4eec\u53ef\u4ee5\u5c06\u6700\u7ec8\u7684\u8ba1\u7b97\u7ed3\u679c\u901a\u8fc7\u5c0f\u63d0\u7434\u56fe\u5c55\u793a\u51fa\u6765\uff0c\u8ba9\u6211\u4eec\u6765\u770b\u770b\u5177\u4f53\u7684\u4ee3\u7801\u5b9e\u73b0\u5427\uff01<\/span><\/p>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">library(vioplot)<\/span><\/code><code><span class=\"code-snippet_outer\">pdf(paste(subgroup,<span class=\"code-snippet__string\">\"Xcell_score.pdf\"<\/span>,sep=<span class=\"code-snippet__string\">\"_\"<\/span>),height=<span class=\"code-snippet__number\">8<\/span>,width=<span class=\"code-snippet__number\">15<\/span>) #\u8bbe\u7f6e\u53ef\u89c6\u5316\u548c\u4fdd\u5b58\u7684\u6587\u4ef6\u540d\u79f0<\/span><\/code><code><span class=\"code-snippet_outer\">par(las=<span class=\"code-snippet__number\">1<\/span>,mar=c(<span class=\"code-snippet__number\">10<\/span>,<span class=\"code-snippet__number\">6<\/span>,<span class=\"code-snippet__number\">3<\/span>,<span class=\"code-snippet__number\">3<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\">x=c(<span class=\"code-snippet__number\">1<\/span>:ncol(rt))<\/span><\/code><code><span class=\"code-snippet_outer\">y=c(<span class=\"code-snippet__number\">1<\/span>:ncol(rt))<\/span><\/code><code><span class=\"code-snippet_outer\">#\u7ed8\u5236\u7a7a\u767d\u6563\u70b9\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\">plot(x,y,<\/span><\/code><code><span class=\"code-snippet_outer\"> xlim=c(<span class=\"code-snippet__number\">0<\/span>,(ncol(rt)<span class=\"code-snippet__number\">-1<\/span>)*<span class=\"code-snippet__number\">3<\/span>+<span class=\"code-snippet__number\">2<\/span>),ylim=c(min(rt),max(rt)+<span class=\"code-snippet__number\">0.02<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> main=<span class=\"code-snippet__string\">\"GSE57065\"<\/span>,xlab=<span class=\"code-snippet__string\">\"\"<\/span>, ylab=<span class=\"code-snippet__string\">\"Xcell score\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> pch=<span class=\"code-snippet__number\">21<\/span>,col=<span class=\"code-snippet__string\">\"white\"<\/span>,xaxt=<span class=\"code-snippet__string\">\"n\"<\/span>,cex.lab=<span class=\"code-snippet__number\">1.3<\/span>,cex.main=<span class=\"code-snippet__number\">1.5<\/span>) <\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__keyword\">for<\/span>(i in <span class=\"code-snippet__number\">1<\/span>:ncol(rt)){<\/span><\/code><code><span class=\"code-snippet_outer\"> normalData=rt[<span class=\"code-snippet__number\">1<\/span>:normal,i]<\/span><\/code><code><span class=\"code-snippet_outer\"> tumorData=rt[(normal+<span class=\"code-snippet__number\">1<\/span>):(normal+tumor),i]<\/span><\/code><code><span class=\"code-snippet_outer\"> wilcoxtest=wilcox.test(normalData,tumorData,exact = F)<\/span><\/code><code><span class=\"code-snippet_outer\"> p=round(wilcoxtest$p.value,<span class=\"code-snippet__number\">3<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> vioplot(normalData,at=<span class=\"code-snippet__number\">3<\/span>*(i<span class=\"code-snippet__number\">-1<\/span>),lty=<span class=\"code-snippet__number\">1<\/span>,add = T,col = <span class=\"code-snippet__string\">'#42B540FF'<\/span>) #\u7ed8\u5236\u6b63\u5e38\u6570\u636e\u7684\u5c0f\u63d0\u7434\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"> vioplot(tumorData,at=<span class=\"code-snippet__number\">3<\/span>*(i<span class=\"code-snippet__number\">-1<\/span>)+<span class=\"code-snippet__number\">1<\/span>,lty=<span class=\"code-snippet__number\">1<\/span>,add = T,col = <span class=\"code-snippet__string\">'#925E9FFF'<\/span>) #\u7ed8\u5236\u80bf\u7624\u6570\u636e\u7684\u5c0f\u63d0\u7434\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"> mx=max(c(normalData,tumorData))<\/span><\/code><code><span class=\"code-snippet_outer\"> lines(c(x=<span class=\"code-snippet__number\">3<\/span>*(i<span class=\"code-snippet__number\">-1<\/span>)+<span class=\"code-snippet__number\">0.2<\/span>,x=<span class=\"code-snippet__number\">3<\/span>*(i<span class=\"code-snippet__number\">-1<\/span>)+<span class=\"code-snippet__number\">0.8<\/span>),c(mx,mx))<\/span><\/code><code><span class=\"code-snippet_outer\"> text(x=<span class=\"code-snippet__number\">3<\/span>*(i<span class=\"code-snippet__number\">-1<\/span>)+<span class=\"code-snippet__number\">1<\/span>,y=mx+<span class=\"code-snippet__number\">0.02<\/span>,labels=ifelse(p<<span class=\"code-snippet__number\">0.001<\/span>,paste0(<span class=\"code-snippet__string\">\"p<0.001\"<\/span>),paste0(<span class=\"code-snippet__string\">\"p=\"<\/span>,p)),cex = <span class=\"code-snippet__number\">0.8<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> }<\/span><\/code><code><span class=\"code-snippet_outer\"> text(seq(<span class=\"code-snippet__number\">1<\/span>,((ncol(rt)<span class=\"code-snippet__number\">-1<\/span>)*<span class=\"code-snippet__number\">3<\/span>+<span class=\"code-snippet__number\">1<\/span>),<span class=\"code-snippet__number\">3<\/span>),<span class=\"code-snippet__number\">-0.01<\/span>,xpd = NA,labels=colnames(rt),cex = <span class=\"code-snippet__number\">1<\/span>,srt = <span class=\"code-snippet__number\">45<\/span>,pos=<span class=\"code-snippet__number\">2<\/span>,font=<span class=\"code-snippet__number\">2<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> dev.off()<\/span><\/code><code><span class=\"code-snippet_outer\"> write.csv(sig,paste(subgroup,<span class=\"code-snippet__string\">\"_sig_cell.csv\"<\/span>,sep=<span class=\"code-snippet__string\">\"\"<\/span>)) #\u4fdd\u5b58\u6700\u7ec8\u7ed3\u679c <\/span><\/code><code><span class=\"code-snippet_outer\">} <\/span><\/code><\/pre>\n<\/section>\n<section style=\"margin-top: 15px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<section>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u73b0\u5728\uff0c\u4f60\u5df2\u7ecf\u6210\u529f\u5b8c\u6210\u4e86xCell\u5206\u6790\u7ec6\u80de\u5230\u53ef\u89c6\u5316\u7684\u5de6\u53f3\u5de5\u4f5c\uff0c\u73b0\u5728\u5c0f\u679c\u7ed9\u5927\u5bb6\u5c55\u793a\u5176\u4e2d\u4e00\u4e2a\u7ec6\u80de\u5b50\u7fa4\u7684\u4e0b\u6e38\u5206\u6790\u53ef\u89c6\u5316\u7ed3\u679c\uff01\u4e00\u8d77\u6765\u770b\u5427\uff01<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.4828767123287671\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_IFHHvkuYvorKoAibEv7ickxRdKALzg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNxTtxS8QqsGGfAicthiasv9ia3jxdyIFHHvkuYvorKoAibEv7ickxRdKALzg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"584\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u600e\u4e48\u6837\uff0c\u4f60\u5b66\u4f1a\u600e\u4e48\u4f7f\u7528xCell\u5305\u4e86\u561b? \u66f4\u591a\u5b66\u4e60\u8d44\u6e90\u8bf7\u5927\u5bb6\u79fb\u6b65\u5c0f\u679c\u4e13\u5c5e\u4e91\u751f\u4fe1\u5e73\u53f0\u641c\u7d22\u66f4\u591a\u8d44\u6e90\u54e6\uff01<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\"><span style=\"font-size: 14px;letter-spacing: 0.578px;\">\u5c0f\u679c\u4e13\u5c5e\u4e91\u751f\u4fe1\u5e73\u53f0<\/span>\uff1a<\/span><em style=\"letter-spacing: 0.578px;color: rgb(0, 0, 0);font-size: 17px;line-height: 1.59em;\"><strong>\u4e91\u751f\u4fe1 – \u5b66\u751f\u7269\u4fe1\u606f\u5b66 (biocloudservice.com)<\/strong><\/em><\/p>\n<p><span style=\"line-height: 2em;font-size: 14px;letter-spacing: 0.5px;caret-color: rgb(255, 0, 0);\"><span style=\"font-size: 14px;letter-spacing: 0.578px;\">\u4e91\u751f\u4fe1\u5e73\u53f0\u4e5f\u6709\u514d\u75ab\u4e13\u7248\u7684\u5b66\u4e60\u6a21\u5757\u54e6\uff0c\u5feb\u6765\u627e\u5230\u4f60\u60f3\u5b66\u4e60\u7684\u4e13\u5c5e\u6a21\u5757\u5427\uff01<\/span>\u5982\u679c\u60f3\u7528\u670d\u52a1\u5668\u53ef\u4ee5\u8054\u7cfb\u5fae\u4fe1\uff1a<\/span><em style=\"letter-spacing: 0.578px;color: rgb(0, 0, 0);font-size: 17px;line-height: 1.59em;\"><strong>18502195490\uff08\u5feb\u6765\u8054\u7cfb\u6211\u4eec\u4f7f\u7528\u5427\uff01\uff09<\/strong><\/em><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section data-tools=\"135\u7f16\u8f91\u5668\" data-id=\"105054\" style=\"font-size: 16px;\">\n<section style=\"margin: 10px auto;text-align: center;\">\n<section style=\"padding-top: 30px;padding-bottom: 30px;background-color: rgb(242, 246, 255);border-radius: 7px;\">\n<section style=\"display: flex;justify-content: space-evenly;align-items: center;\">\n<section data-width=\"26%\" style=\"width: 26%;max-width: 26% !important;\">\n<section style=\"padding: 7px;border-radius: 15px;box-shadow: rgba(169, 187, 223, 0.45) 4px 4px 8px, rgb(255, 255, 255) -2.5px -2.5px 5px;\">\n<section data-width=\"100%\" style=\"width: 100%;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.005281690140845\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_0WFLKBMicbm6iawtkrNrMxpSVU9GBQ-10.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtN261llpmfF6btPBTUKkibEdnRz5Y0WFLKBMicbm6iawtkrNrMxpSVU9GBQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"568\" data-width=\"100%\" style=\"vertical-align: inherit;width: 100%;display: block;border-radius: 7px;\" title=\"\u5c0f\u4e91-\u5fae\u4fe1\u4e8c\u7ef4\u7801.png\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section data-width=\"62%\" style=\"width: 62%;max-width: 62% !important;\">\n<section style=\"display: inline-block;\">\n<section style=\"padding: 7px 15px;border-radius: 20px;box-shadow: rgba(169, 187, 223, 0.45) 6px 6px 8px inset, rgb(255, 255, 255) -2.5px -2.5px 5px inset;\">\n<section data-brushtype=\"text\" style=\"font-size: 14px;letter-spacing: 1.5px;color: rgb(111, 133, 172);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u5fae\u4fe1\u53f7 | 18502195490<\/span><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-top: 12px;padding: 7px 15px;border-radius: 20px;box-shadow: rgba(169, 187, 223, 0.45) 6px 6px 8px inset, rgb(255, 255, 255) -2.5px -2.5px 5px inset;\">\n<section data-brushtype=\"text\" style=\"font-size: 14px;letter-spacing: 1.5px;color: rgb(111, 133, 172);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u77e5\u4e4e | \u751f\u4fe1\u679c<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"letter-spacing: 0.578px;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-top: 0.5em;\">\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);\">\n<section style=\"text-align: left;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;\">\n<section style=\"margin-bottom: 8px;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u70b9\u51fb<strong><span style=\"color: rgb(0, 150, 136);\">\u201c\u9605\u8bfb\u539f\u6587\u201d<\/span><\/strong>\u7acb\u523b\u62e5\u6709<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 136);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u2193\u2193\u2193 <\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>xCell\uff1a\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u7684\u514d\u75ab\u6d78\u6da6\u5206\u6790\u4e13\u5bb6\uff01 \u539f\u521b \u5c0f\u679c \u751f\u4fe1\u679c& […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/54165"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=54165"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/54165\/revisions"}],"predecessor-version":[{"id":54177,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/54165\/revisions\/54177"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=54165"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=54165"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=54165"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}