{"id":54418,"date":"2024-06-16T14:12:07","date_gmt":"2024-06-16T06:12:07","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=54418"},"modified":"2024-06-16T14:12:07","modified_gmt":"2024-06-16T06:12:07","slug":"20%e5%88%86%e6%96%87%e7%ab%a0%e7%9a%84%e5%8d%95%e7%bb%86%e8%83%9e%e7%83%ad%e5%9b%be%ef%bc%81%ef%bc%81%e8%bf%98%e4%b8%8d%e8%b7%9f%e5%b0%8f%e6%9e%9c%e5%8e%bb%e5%ad%a6%e4%b9%a0%e4%b8%80%e4%b8%8b%e5%a6%82","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=54418","title":{"rendered":"20\u5206\u6587\u7ae0\u7684\u5355\u7ec6\u80de\u70ed\u56fe\uff01\uff01\u8fd8\u4e0d\u8ddf\u5c0f\u679c\u53bb\u5b66\u4e60\u4e00\u4e0b\u5982\u4f55\u7ed8\u5236\uff01"},"content":{"rendered":"


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\n<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 60px}.rich_media_area_extra{padding:0 60px 21px 60px}}@media (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner,body{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media{padding:20px;overflow:hidden;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}@media screen and (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media_content{overflow:hidden;color:#333;font-size:17px;word-wrap:break-word;-webkit-hyphens:auto;-ms-hyphens:auto;hyphens:auto;text-align:justify;position:relative;z-index:0}.rich_media_content *{max-width:100%!important;box-sizing:border-box!important;-webkit-box-sizing:border-box!important;word-wrap:break-word!important}.rich_media_content p{clear:both;min-height:1em}.rich_media_content em{font-style:italic}.rich_media_content fieldset{min-width:0}.rich_media_content .list-paddingleft-1,.rich_media_content .list-paddingleft-2,.rich_media_content .list-paddingleft-3{padding-left:2.2em}.rich_media_content .list-paddingleft-1 .list-paddingleft-2,.rich_media_content .list-paddingleft-2 .list-paddingleft-2,.rich_media_content .list-paddingleft-3 .list-paddingleft-2{padding-left:30px}.rich_media_content .list-paddingleft-1{padding-left:1.2em}.rich_media_content .list-paddingleft-3{padding-left:3.2em}.rich_media_content .code-snippet,.rich_media_content .code-snippet__fix{max-width:1000%!important}.rich_media_content .code-snippet *,.rich_media_content .code-snippet__fix *{max-width:1000%!important}.rich_media_title{font-size:22px;line-height:1.4;margin-bottom:13px;padding-bottom:13px;border-bottom:1px solid #e7e7eb;}@supports(-webkit-overflow-scrolling:touch){.rich_media_title{font-weight:700}}.rich_media_meta{display:inline-block;vertical-align:middle;padding:0 0 10px 0;font-size:15px;-webkit-tap-highlight-color:rgba(0,0,0,0)}.rich_media_meta.icon_appmsg_tag{margin-right:0px}.rich_media_meta.meta_tag_text{margin-right:0}.rich_media_meta_list em{font-style:normal}.rich_media_meta_text{color:#a5a5a5;}p{margin:0;}.msgBox{margin-top:20px;padding-top:20px;padding-left:50px;overflow:hidden;border-top:2px dashed #09a2ff;}.msg{padding-top:7px;clear:both;}.msgBody{float:right;width:100%;margin-left:55px;padding-bottom:15px;border-bottom:1px dashed #e0e0e0;}.userHeadImg{float:left;margin-left:-50px;}.userHeadImg img{width:40px;height:40px;margin-right:10px;border-radius:3px;}.userName{color:#888888;line-height:24px;font-size:14px;margin:5px 0 5px 0;height:24px;}.replyBody,.autherBody{color:#565656;font-size:15px;}.replyIcon{border-left:4px solid #33ab01;margin-right:5px;}.ad{text-decoration:none;color:#d6d4d4;font-size:12px;}.msgBodyReply{padding-top:5px;}.userName span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation 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0;align-items:center}.flex_bd{padding-left:14px;}.account_nickname{display:inline-block;vertical-align:middle;line-height:1.2;color:#576b95;font-size:14px}.account_desc{overflow:hidden;text-overflow:ellipsis;display:-webkit-box;-webkit-box-orient:vertical;-webkit-line-clamp:1;color:rgba(0,0,0,0.3);font-size:14px;line-height:1.2;padding-top:.4em}.msg_source_url{text-align:left;word-break:break-all;margin-top:20px;}.msg_source_url a{padding-right:10px;}.msg_source_url .url_text{color:#a8a8a8;}.video-desc{font-size:14px;margin-top:15px;color:#6c6c6c;}.msg_source_url{text-align:left;}.original_primary_card_tips{color:rgba(0,0,0,0.3);line-height:1.4;font-size:15px;}.weui-flex__item{margin-bottom:20px;padding:20px 16px;margin-top:16px;line-height:1.4;align-items:center;background-color:#f7f7f7;border-radius:8px;position:relative;}.original_primary_desc{color:rgba(0,0,0,0.5);font-size:14px;padding-top:4px;width:auto;overflow:hidden;text-overflow:ellipsis;}.msgBodyReplyList{border-top:1px solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar 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href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2821072943068839938#wechat_redirect\" title=\"\u751f\u4fe1\u7ed8\u56fe\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u7ed8\u56fe<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2664545260517294084#wechat_redirect\" title=\"\u751f\u4fe1\u5b9e\u64cd\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u5b9e\u64cd<\/a><\/p>\n<\/div>\n<p data-mpa-powered-by=\"yiban.io\"><span style=\"letter-spacing: 0.034em;font-size: 14px;\">\u5c0f\u679c\u6700\u8fd1\u5728\u770b\u6587\u732e\u7684\u65f6\u5019\uff0c\u53d1\u73b0\u4e00\u4e2aIF>20\uff01\u6587\u7ae0\u4e2d\u6709\u4e00\u5f20\u56fe\u662f\u6709\u5173\u5355\u7ec6\u80de\u7684\u70ed\u56fe\uff0c\u56fe\u7247\u4e2d\u662fmarker\u57fa\u56e0\u7684\u70ed\u56fe\u5916\u52a0+Go\u5bcc\u96c6\u6761\u5f62\u56fe\uff0c\u6211\u4eec\u5148\u770b\u4e00\u4e0b\u56fe\u7247<\/span><br \/><\/p>\n<p><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.2876404494382023\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_aHleic3iav5SyiaTWzf3KZXiaP9jEA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAykBnUQkD3oVCjXDv2szbiaZor8fVIwlL89vTW2DRzqd7icufm93ViaHleic3iav5SyiaTWzf3KZXiaP9jEA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"445\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-size: 14px;letter-spacing: 0.034em;\">\u8fd9\u4e2a\u56fe\u7247\u4e2d\u5de6\u56fe\u663e\u793a\u4e86\u6bcf\u79cd\u7ec6\u80de\u7c7b\u578b\u6309LogFC\u6392\u540d\u7684\u524d50\u4e2a\u57fa\u56e0\u7684\u8868\u8fbe\u8c31\u3002\u53f3\uff0c\u6bcf\u79cd\u7ec6\u80de\u7c7b\u578b\u7684\u6807\u8bb0\u57fa\u56e0\u7684\u4e30\u5bccGO\u672f\u8bed\uff0c\u8fd9\u4e2a\u56fe\u53ef\u4ee5\u5c55\u793a\u5f88\u591a\u7684\u4fe1\u606f\uff0c\u5c0f\u4f19\u4f34\u5b66\u4f1a\u8fd9\u6837\u56fe\u5c31\u53ef\u4ee5\u628a\u81ea\u5df1\u5355\u7ec6\u80de\u6570\u636e\u8fd8\u6709\u5bcc\u96c6\u5206\u6790\u653e\u8fdb\u53bb\u3002\u90a3\u4e48\u4eca\u5929\u5c0f\u679c\u5c31\u5e26\u5c0f\u4f19\u4f34\u590d\u73b0\u4e00\u4e0b\u8fd9\u5f20\u56fe\u3002<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3888888888888889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_SoZzbcOiaYpvj4X1dCVHQkCg2mw6tQ.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAyuYsqlJnQUJvnS2iciaDibtfbzPbUvcAaXJkxdF3M7b5psgPl2SoZzbcOiaYpvj4X1dCVHQkCg2mw6tQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"\" data-recalc-dims=\"1\" \/><span style=\"font-size: 14px;letter-spacing: 0.034em;text-align: justify;\"><\/span><\/p>\n<p><span style=\"font-size: 14px;\">\u6211\u4eec\u4f7f\u7528R\u5305\u4e2d\u81ea\u5e26\u7684\u6570\u636e\uff0c\u9009\u62e9\u524d5\u540d\u7684marker\u57fa\u56e0\u53bb\u8fdb\u884c\u70ed\u56fe\u7ed8\u5236\u3002<\/span><\/p>\n<p><span style=\"font-size: 14px;\">\u6570\u636e\u6765\u81eamarker\u57fa\u56e0\u6570\u636e\u5462\u6765\u81eascRNAtoolVis\u3002\u6211\u4eec\u5148\u4e0b\u8f7d\u4e00\u4e0b\u8fd9\u4e2a\u5305<\/span><\/p>\n<p><span style=\"font-size: 14px;\"><em style=\"letter-spacing: 0.578px;white-space: normal;color: rgb(178, 178, 178);\"><span style=\"font-size: 13px;text-decoration: underline;\">\u516c\u4f17\u53f7\u540e\u53f0\u56de\u590d\u201c111″,\u9886\u53d6\u4ee3\u7801\uff0c\u4ee3\u7801\u7f16\u53f7\uff1a231012<\/span><\/em><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\">#if not install ggunchull<\/span><\/code><code><span class=\"code-snippet_outer\">#devtools::install_github(\"sajuukLyu\/ggunchull\", type = \"source\")<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#install.packages('devtools')<\/span><\/code><code><span class=\"code-snippet_outer\">#devtools::install_github('junjunlab\/scRNAtoolVis')<\/span><\/code><\/pre>\n<\/section>\n<p style=\"font-size: 14px;\"><span style=\"font-size: 14px;\">\u6211\u4eec\u5148\u770b\u4e00\u4e0b\u6570\u636e<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code><span class=\"code-snippet_outer\">library(scRNAtoolVis)<\/span><\/code><code><span class=\"code-snippet_outer\">httest <- system.file(\"extdata\", \"htdata.RDS\", package = \"scRNAtoolVis\")<\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- readRDS(httest)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># load markergene<\/span><\/code><code><span class=\"code-snippet_outer\">markergene <- system.file(\"extdata\", \"top5pbmc.markers.csv\", package = \"scRNAtoolVis\")<\/span><\/code><code><span class=\"code-snippet_outer\">markers <- read.table(markergene, sep = ',', header = TRUE)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">dim(markers)<\/span><\/code><\/pre>\n<\/section>\n<p><img decoding=\"async\" data-ratio=\"0.26973684210526316\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_XweK3cJLdNUlRic8tUAxm2c7kibkVQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAykBnUQkD3oVCjXDv2szbiaZRt1STyGyINQrOjr2tVNyqNjRveXweK3cJLdNUlRic8tUAxm2c7kibkVQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"152\" data-recalc-dims=\"1\" \/><\/p>\n<p><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5072202166064982\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_WhYaibZU7LDRhTXzrcuxBt3hrbGupw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAykBnUQkD3oVCjXDv2szbiaZwPGXafQRJaC6HdzVnuErSQPGAWhYaibZU7LDRhTXzrcuxBt3hrbGupw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"font-size: 14px;\"><span style=\"font-size: 14px;\">\u6211\u4eec\u5148\u7ed8\u5236\u70ed\u56fe\uff0c\u6570\u636e\u5462\u5176\u5b9e\u5c31\u662f\u6bcf\u4e2a\u7ec6\u80de\u4e9a\u7fa4\u4e2d\u6392\u540d\u524d\u63d0marker\u57fa\u56e0\u5e76\u96c6\u7684\u8868\u8fbe\u70ed\u56fe\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code><span class=\"code-snippet_outer\">library(Seurat)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># AverageExpression \u8ba1\u7b97\u5747\u503c<\/span><\/code><code><span class=\"code-snippet_outer\">df <- as.data.frame(AverageExpression(object = pbmc)$RNA)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">df %>%<\/span><\/code><code><span class=\"code-snippet_outer\">filter(row.names(.) %in% markers$gene) %>%<\/span><\/code><code><span class=\"code-snippet_outer\">rownames_to_column(var = \"gene\") %>%<\/span><\/code><code><span class=\"code-snippet_outer\">right_join(markers) %>%<\/span><\/code><code><span class=\"code-snippet_outer\">arrange(cluster, avg_log2FC) %>%<\/span><\/code><code><span class=\"code-snippet_outer\">pivot_longer(cols = `Naive CD4 T`:`Platelet`, names_to = \"cluster_name\", values_to = \"exp\") %>%<\/span><\/code><code><span class=\"code-snippet_outer\">group_by(gene) %>%<\/span><\/code><code><span class=\"code-snippet_outer\">mutate(exp = as.numeric(scale(exp))) %>%<\/span><\/code><code><span class=\"code-snippet_outer\">ungroup() -> df<\/span><\/code><\/pre>\n<\/section>\n<p><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3784530386740331\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_OhTatU12m8uyvX2fwU6ldbiaUjcY7g.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAykBnUQkD3oVCjXDv2szbiaZiagbW9PraNr9ick3avk0z5boGFibOhTatU12m8uyvX2fwU6ldbiaUjcY7g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"724\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"font-size: 14px;\"><span style=\"font-size: 14px;\">\u7ed8\u5236\u70ed\u56fe\uff1a<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">##\u70ed\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u4e00\u51719\u4e2a\u4e9a\u7fa4\uff0c\u8fd9\u91cc\u53ea\u5c55\u793a8\u4e2a\uff0c\u4e0d\u542bNK<\/span><\/code><code><span class=\"code-snippet_outer\">cluster_name <- c(\"Naive CD4 T\", \"CD14+ Mono\", \"Memory CD4 T\", \"B\", \"CD8 T\", \"FCGR3A+ Mono\", \"DC\", \"Platelet\")<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">df %>%<\/span><\/code><code><span class=\"code-snippet_outer\">filter(cluster_name != \"NK\", # \u8fc7\u6ee4\u6389NK\u7ec6\u80de\u7fa4\/\u6ca1\u6709\u4efb\u4f55\u610f\u4e49<\/span><\/code><code><span class=\"code-snippet_outer\">cluster != 6) %>%<\/span><\/code><code><span class=\"code-snippet_outer\">ggplot(aes(x = cluster_name,<\/span><\/code><code><span class=\"code-snippet_outer\">y = reorder(gene, -cluster),<\/span><\/code><code><span class=\"code-snippet_outer\">fill = exp)) +<\/span><\/code><code><span class=\"code-snippet_outer\">geom_tile() +<\/span><\/code><code><span class=\"code-snippet_outer\">scale_fill_gradient2(low = \"blue\", mid = \"white\", high = \"red\") +<\/span><\/code><code><span class=\"code-snippet_outer\">scale_x_discrete(limits = cluster_name)<\/span><\/code><\/pre>\n<\/section>\n<p><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.0245022970903521\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_C20KXfyxMaricQbXIEib02UOjpYYyQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAykBnUQkD3oVCjXDv2szbiaZ0bXIQAicQuysY2SPPj9lt7SkBBuC20KXfyxMaricQbXIEib02UOjpYYyQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"653\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"font-size: 14px;\"><span style=\"font-size: 14px;\">\u4e00\u4e2a\u7b80\u5355\u7684\u70ed\u56fe\u6211\u4eec\u5c31\u7ed8\u5236\u51fa\u6765\u4e86\uff0c\u6211\u4eec\u5728\u6765\u8c03\u6574\u4e00\u4e0b\u7ec6\u8282\uff0c\u53bb\u6389\u6a2a\u7eb5\u5750\u6807\u8fd8\u6709\u523b\u5ea6\uff0c\u5728\u4fee\u6539\u4e00\u4e0b\u56fe\u4f8b\u7684\u4f4d\u7f6e\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">p1 <- df %>%<\/span><\/code><code><span class=\"code-snippet_outer\">filter(cluster_name != \"NK\",<\/span><\/code><code><span class=\"code-snippet_outer\">cluster != 6) %>%<\/span><\/code><code><span class=\"code-snippet_outer\">ggplot(aes(x = cluster_name,<\/span><\/code><code><span class=\"code-snippet_outer\">y = reorder(gene, -cluster),<\/span><\/code><code><span class=\"code-snippet_outer\">fill = exp)) +<\/span><\/code><code><span class=\"code-snippet_outer\">geom_tile(color = \"grey60\") +<\/span><\/code><code><span class=\"code-snippet_outer\">scale_fill_gradient2(low = \"blue\", mid = \"white\", high = \"red\", breaks = c(-1,0,1,2)) +<\/span><\/code><code><span class=\"code-snippet_outer\">scale_x_discrete(limits = cluster_name) +<\/span><\/code><code><span class=\"code-snippet_outer\">theme_void() +<\/span><\/code><code><span class=\"code-snippet_outer\">theme(legend.position = \"left\")<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">p1<\/span><\/code><\/pre>\n<\/section>\n<p><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.0196078431372548\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_MsovmLCtTRibbibIqkjN3U3WicGPaQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAykBnUQkD3oVCjXDv2szbiaZ6hCFeX221GcUiaz9XpdRxcsF7dFIMsovmLCtTRibbibIqkjN3U3WicGPaQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"663\" data-recalc-dims=\"1\"><\/p>\n<p style=\"font-size: 14px;\"><span style=\"font-size: 14px;\">\u8fd9\u6837\u4e00\u6765\u6587\u732e\u4e2d\u5de6\u8fb9\u7684\u56fe\u5927\u81f4\u7ed8\u5236\u51fa\u6765\u4e86\uff0c\u6211\u4eec\u4e00\u70b9\u4e00\u70b9\u7684\u7ed8\u5236\uff0c\u4e0b\u9762\u6211\u4eec\u7ed8\u5236\u70b9\u56fe\uff0c<\/span><\/p>\n<p style=\"font-size: 14px;\"><span style=\"font-size: 14px;\">\u70b9\u56fe\u4f5c\u4e3a\u70ed\u56fe\u7684\u6bcf\u4e00\u5217\u7ec6\u80de\u4e9a\u7fa4\uff08\u540d\u79f0\uff09\u6ce8\u91ca<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\">#\u70b9\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\">colors <- c(\"#4A9D47\", \"#F19294\", \"#E45D61\", \"#96C3D8\", \"#5F9BBE\", \"#F5B375\", \"#67A59B\", \"#A5D38F\")<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">df2 <- data.frame(x = 0, y = levels(pbmc)[-7], stringsAsFactors = F)<\/span><\/code><code><span class=\"code-snippet_outer\">df2$y <- factor(df2$y, levels = df2$y)<\/span><\/code><\/pre>\n<\/section>\n<p><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.0225225225225225\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_DYK2Um0EJibSjIkjAXQzHf95pBTp1g.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAykBnUQkD3oVCjXDv2szbiaZVJgKCibRCwgZibQ11skxWO1XxhMDYK2Um0EJibSjIkjAXQzHf95pBTp1g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"222\" data-recalc-dims=\"1\"><\/p>\n<p style=\"font-size: 14px;\"><span style=\"font-size: 14px;\">\u4e0b\u9762\u7ed8\u5236\u70b9\u56fe\uff1a<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">#\u70b9\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\">p2 <- df2 %>%<\/span><\/code><code><span class=\"code-snippet_outer\">ggplot(aes(x = y, y = 1, color = factor(y))) +<\/span><\/code><code><span class=\"code-snippet_outer\">geom_point(size = 9, show.legend = F) +<\/span><\/code><code><span class=\"code-snippet_outer\">scale_color_manual(values = colors) +<\/span><\/code><code><span class=\"code-snippet_outer\">scale_y_continuous(expand = c(0,0)) +<\/span><\/code><code><span class=\"code-snippet_outer\">theme(legend.position = \"none\",<\/span><\/code><code><span class=\"code-snippet_outer\">panel.spacing = unit(0, \"lines\"),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.background = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.border = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">plot.background = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">plot.margin = margin(0, 0, 0, 0, \"pt\"),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.text.x = element_text(angle = 90,<\/span><\/code><code><span class=\"code-snippet_outer\">size = 12,<\/span><\/code><code><span class=\"code-snippet_outer\">hjust = 1,<\/span><\/code><code><span class=\"code-snippet_outer\">vjust = 0.5,<\/span><\/code><code><span class=\"code-snippet_outer\">color = \"black\"),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.title = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.ticks = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.grid = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.text.y = element_blank())<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">p2<\/span><\/code><\/pre>\n<\/section>\n<p><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7488514548238897\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_1P2Dibib7iaqXct9rnrfiaiaXAJDpg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAykBnUQkD3oVCjXDv2szbiaZMBMkOpQ7eVib6NqgMAwc7uklbbW8jS1P2Dibib7iaqXct9rnrfiaiaXAJDpg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"653\" data-recalc-dims=\"1\"><\/p>\n<p style=\"font-size: 14px;\"><span style=\"font-size: 14px;\">\u597d\u4e86\u70b9\u56fe\u7684\u7ed8\u5236\u6211\u4eec\u5c31\u7ed8\u5236\u5b8c\u6210\u4e86\uff0c\u4e0b\u9762\u6211\u4eec\u53bb\u7ed8\u5236\u6761\u5f62\u56fe\uff0c\u5c31\u662fGo\u5bcc\u96c6\u7684\u7ed3\u679c\uff0c\u6211\u4eec\u6ca1\u6709\u627e\u5bcc\u96c6\u7ed3\u679c\uff0c\u5c0f\u679c\u8fd9\u91cc\u6a21\u62df\u5bcc\u96c6\u7ed3\u679c\uff0c\u5c0f\u4f19\u4f34\u81ea\u5df1\u7684\u5bcc\u96c6\u7ed3\u679c\u653e\u8fdb\u53bb\u5c31\u884c\uff0c\u5c31\u662f\u5bf9\u4f60\u6bcf\u4e2a\u4e9a\u7fa4\u7684topmarker\u57fa\u56e0\u8fdb\u884c\u5bcc\u96c6\u5206\u6790<\/span><\/p>\n<p style=\"font-size: 14px;\"><span style=\"font-size: 14px;\">\u6574\u7406\u6210\u4e0b\u9762\u7684\u683c\u5f0f\uff0c\u6765\u770b\u4e00\u4e0b\uff0c\u6bcf\u4e2a\u4e9a\u7fa4\u5c55\u793a2\u4e2a\u4ee3\u8868\u6027\u7684Goterm<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"properties\"><code><span class=\"code-snippet_outer\">##\u6761\u5f62\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">enrich_res <- \"Cluster GO_term Log10(padj)<\/span><\/code><code><span class=\"code-snippet_outer\">Naive CD4 T GO:00001 -20.00<\/span><\/code><code><span class=\"code-snippet_outer\">CD14+ Mono GO:00002 -18.00<\/span><\/code><code><span class=\"code-snippet_outer\">Memory CD4 T GO:00003 -14.00<\/span><\/code><code><span class=\"code-snippet_outer\">B GO:00004 -12.00<\/span><\/code><code><span class=\"code-snippet_outer\">CD8 T GO:00005 -19.00<\/span><\/code><code><span class=\"code-snippet_outer\">FCGR3A+ Mono GO:00006 -17.00<\/span><\/code><code><span class=\"code-snippet_outer\">DC GO:00008 -18.00<\/span><\/code><code><span class=\"code-snippet_outer\">Platelet GO:00009 -13.00<\/span><\/code><code><span class=\"code-snippet_outer\">Naive CD4 T GO:00010 -12.00<\/span><\/code><code><span class=\"code-snippet_outer\">CD14+ Mono GO:00011 -10.00<\/span><\/code><code><span class=\"code-snippet_outer\">Memory CD4 T GO:00012 -6.00<\/span><\/code><code><span class=\"code-snippet_outer\">B GO:00013 -11.00<\/span><\/code><code><span class=\"code-snippet_outer\">CD8 T GO:00014 -12.00<\/span><\/code><code><span class=\"code-snippet_outer\">FCGR3A+ Mono GO:00015 -9.00<\/span><\/code><code><span class=\"code-snippet_outer\">DC GO:00017 -8.00<\/span><\/code><code><span class=\"code-snippet_outer\">Platelet GO:00018 -8.00\"<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">df3 <- read.table(textConnection(enrich_res),<\/span><\/code><code><span class=\"code-snippet_outer\">sep = \"t\", header = T,check.names = F)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">df3$Cluster <span class=\"code-snippet__string\"><- factor(df3$Cluster, levels = levels(pbmc)[-7])<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><img decoding=\"async\" data-ratio=\"1.5229007633587786\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_FGodcFlffqISr1R8aY9cpYH4vUJY1w.png?w=640\" 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