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style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u751f\u4fe1\u4ebaR\u8bed\u8a00\u5b66\u4e60\u5fc5\u5907<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u7acb\u523b\u62e5\u6709\u4e00\u4e2aRstudio\u8d26\u53f7<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u5f00\u542f\u5347\u7ea7\u6a21\u5f0f\u5427<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"color: rgb(255, 129, 36);font-size: 12px;letter-spacing: 0.5px;\">\uff0856\u7ebf\u7a0b\uff0c256G\u5185\u5b58\uff0c\u4e2a\u4eba\u5b58\u50a81T\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 29.9652%;align-self: flex-start;flex: 0 0 auto;height: 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data-type=\"png\" data-w=\"400\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"font-size: var(--articleFontsize);letter-spacing: 0.578px;text-align: justify;\"><\/span><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section data-role=\"list\" style=\"\">\n<section powered-by=\"xiumi.us\" style=\"\">\n<section style=\"\">\n<section powered-by=\"xiumi.us\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 8px;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 0.578px;text-wrap: wrap;background-color: rgb(255, 255, 255);font-size: 16px;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;visibility: visible;\">\n<section style=\"padding-left: 8px;outline: 0px;display: inline-block;width: 578px;vertical-align: top;border-left: 3px solid rgb(219, 219, 219);border-bottom-left-radius: 0px;align-self: flex-start;flex: 0 0 auto;visibility: visible;\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;color: rgba(0, 0, 0, 0.5);text-align: justify;visibility: visible;\">\n<p style=\"outline: 0px;visibility: visible;\"><span style=\"outline: 0px;font-size: 14px;visibility: visible;\">\u8fd8\u5728\u4e3a\u5982\u4f55\u5165\u95e8\u751f\u4fe1\u800c\u5f77\u5fa8\u5417\uff1f\u8fd8\u5728\u4e3aLinux\u7cfb\u7edf\u800c\u611f\u5230\u65e0\u52a9\u5417\uff1f\u5feb\u5173\u6ce8\u5c0f\u679c\u7684\u5fae\u4fe1\u516c\u4f17\u53f7\uff0c\u5c0f\u679c\u624b\u628a\u624b\u5e26\u4f60\u5165\u95e8\u751f\u4fe1\uff0c\u6ca1\u6709\u95e8\u69db\u3001\u6ca1\u6709\u96be\u5ea6\uff0c\u8ddf\u7740\u6548\u679c\u8d70\uff0c\u5565\u95ee\u9898\u90fd\u6ca1\u6709\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<p><br \/><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14px;color: rgb(51, 51, 51);font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">hello<span style=\"color: rgb(51, 51, 51);\">\uff0c\u4eca\u5929\u5c0f\u679c\u6559\u5927\u5bb6\u5982\u4f55\u4f7f\u7528DESeq2\u5305\u5bf9counts\u8868\u8fbe\u77e9\u9635\u8fdb\u884c\u5dee\u5f02\u57fa\u56e0\u5206\u6790\uff0c\u5e76\u5bf9\u5206\u6790\u7ed3\u679c\u7ed8\u5236\u706b\u5c71\u56fe\u5b9e\u73b0\u5dee\u5f02\u57fa\u56e0\u5206\u6790\u7684\u53ef\u89c6\u5316\uff0c\u5982\u679c\u4f60\u611f\u5174\u8da3\u7684\u8bdd\u5c31\u548c\u5c0f\u679c\u4e00\u8d77\u770b\u4e0b\u53bb\u5427\uff01<\/span><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"color: rgb(51, 51, 51);font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u9996\u5148\u5c0f\u679c\u5148\u6765\u7b80\u5355\u4ecb\u7ecd\u4e00\u4e0b\u8fd9\u4e2a\u5305\uff1a<\/span><span style=\"mso-spacerun:'yes';font-family:'Open Sans';mso-fareast-font-family:\u5b8b\u4f53;color:rgb(51,51,51);font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<section data-role=\"title\" data-tools=\"135\u7f16\u8f91\u5668\" data-id=\"104726\" style=\"margin-bottom: 0px;font-size: 16px;letter-spacing: 0.578px;text-wrap: wrap;\">\n<section style=\"margin: 10px auto;text-align: center;\">\n<section style=\"display: inline-block;\">\n<section style=\"padding: 7px 10px;background-color: rgb(84, 141, 212);\">\n<section style=\"display: flex;justify-content: center;align-items: center;\">\n<section style=\"width: 8px;height: 8px;background-color: rgb(255, 255, 255);border-radius: 50%;overflow: hidden;\"><br \/><\/section>\n<section data-brushtype=\"text\" style=\"margin-right: 10px;margin-left: 10px;letter-spacing: 1.5px;color: rgb(255, 255, 255);\"><strong>\u4e86\u89e3DESeq2<\/strong><\/section>\n<section style=\"width: 8px;height: 8px;background-color: rgb(255, 255, 255);border-radius: 50%;\"><br \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p><span style=\"font-family: "Open Sans";color: rgb(51, 51, 51);font-size: 14px;\">DESeq2<span style=\"color: rgb(51, 51, 51);font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u662f\u4e00\u4e2aR\u8bed\u8a00\u4e2d\u5e38\u7528\u7684\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u5305\uff0c\u7528\u4e8e\u5206\u6790\u9ad8\u901a\u91cf\u6d4b\u5e8f\u6570\u636e\u4e2d\u7684\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u3002\u5b83\u662fBioconductor\u9879\u76ee\u7684\u4e00\u90e8\u5206\uff0c\u63d0\u4f9b\u4e86\u4e00\u5957\u7edf\u8ba1\u65b9\u6cd5\u548c\u529f\u80fd\uff0c\u7528\u4e8e\u68c0\u6d4b\u57fa\u56e0\u5728\u4e0d\u540c\u6761\u4ef6\u4e0b\u7684\u8868\u8fbe\u5dee\u5f02\uff0c\u5e76\u627e\u51fa\u5177\u6709\u751f\u7269\u5b66\u610f\u4e49\u7684\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u3002DESeq2\u5305\u7684\u8bbe\u8ba1\u76ee\u6807\u662f\u9488\u5bf9RNA-seq\u6570\u636e\u8fdb\u884c\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff0c\u5e76\u4e14\u80fd\u591f\u5904\u7406\u5177\u6709\u4f4e\u590d\u5236\u6570\u3001\u9ad8\u53d8\u5f02\u6027\u548c\u6709\u504f\u5dee\u7684\u6570\u636e\u3002\u5b83\u91c7\u7528\u4e86\u8d1f\u4e8c\u9879\u5206\u5e03\u6a21\u578b\u548c\u5e7f\u4e49\u7ebf\u6027\u6a21\u578b\uff08GLM<\/span><span style=\"color: rgb(51, 51, 51);font-family: \u5b8b\u4f53;\">\uff09\u7684\u65b9\u6cd5\uff0c\u901a\u8fc7\u8003\u8651\u6837\u672c\u4e4b\u95f4\u7684\u6280\u672f\u548c\u751f\u7269\u5b66\u53d8\u5f02\uff0c\u8fdb\u884c\u51c6\u786e\u7684\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u3002<\/span><o:p><\/o:p><\/span><\/p>\n<p style=\"font-size: 16px;\"><br \/><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">DESeq2\u5305\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u529f\u80fd\uff0c\u5305\u62ec\uff1a<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;color: rgb(0, 122, 170);\">1.\u6570\u636e\u5bfc\u5165<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\uff1a\u652f\u6301\u4ece\u4e0d\u540c\u7684\u6570\u636e\u6e90\u5bfc\u5165RNA-seq\u6570\u636e\uff0c\u5305\u62ec\u539f\u59cb\u8ba1\u6570\u6570\u636e\u548c\u5df2\u7ecf\u8fdb\u884c\u4e86\u57fa\u56e0\u6ce8\u91ca\u7684\u6570\u636e\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;color: rgb(0, 122, 170);\">2.\u6570\u636e\u9884\u5904\u7406<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\uff1a\u5305\u62ec\u6570\u636e\u89c4\u8303\u5316\u3001\u53bb\u9664\u4f4e\u8868\u8fbe\u57fa\u56e0\u3001\u53bb\u9664\u6279\u6b21\u6548\u5e94\u7b49\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;color: rgb(0, 122, 170);\">3.\u5dee\u5f02\u8868\u8fbe\u5206\u6790<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\uff1a\u4f7f\u7528\u8d1f\u4e8c\u9879\u5206\u5e03\u6a21\u578b\u548cGLM\u8fdb\u884c\u7edf\u8ba1\u5206\u6790\uff0c\u8ba1\u7b97\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u663e\u8457\u6027\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;color: rgb(0, 122, 170);\">4.\u591a\u7ec4\u5b9e\u9a8c\u8bbe\u8ba1<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\uff1a\u652f\u6301\u591a\u7ec4\u6761\u4ef6\u4e0b\u7684\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff0c\u5305\u62ec\u4e24\u7ec4\u6bd4\u8f83\u3001\u591a\u7ec4\u6bd4\u8f83\u548c\u65f6\u95f4\u5e8f\u5217\u5206\u6790\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;color: rgb(0, 122, 170);\"><strong><span style=\"text-decoration: underline;font-size: 14px;\">5.\u57fa\u56e0\u6ce8\u91ca\u548c\u529f\u80fd\u5bcc\u96c6\u5206\u6790<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\uff1a\u652f\u6301\u5c06\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u8fdb\u884c\u6ce8\u91ca\uff0c\u5e76\u8fdb\u884c\u529f\u80fd\u5bcc\u96c6\u5206\u6790\uff0c\u4ee5\u4e86\u89e3\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u751f\u7269\u5b66\u529f\u80fd\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;color: rgb(0, 122, 170);\">6.\u53ef\u89c6\u5316<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\uff1aDESeq2\u5305\u96c6\u6210\u4e86ggplot2\u548c\u5176\u4ed6\u53ef\u89c6\u5316\u5de5\u5177\uff0c\u53ef\u4ee5\u65b9\u4fbf\u5730\u7ed8\u5236\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u56fe\u8868\uff0c\u5982\u706b\u5c71\u56fe\u3001MA\u56fe\u7b49\u3002<\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:'Open Sans';mso-fareast-font-family:\u5b8b\u4f53;color:rgb(51,51,51);font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><br \/><\/o:p><\/span><\/p>\n<section data-role=\"title\" data-tools=\"135\u7f16\u8f91\u5668\" data-id=\"104726\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;text-wrap: wrap;font-size: 16px;\">\n<section style=\"margin: 10px auto;text-align: center;\">\n<section style=\"display: inline-block;\">\n<section style=\"padding: 7px 10px;background-color: rgb(84, 141, 212);\">\n<section style=\"display: flex;justify-content: center;align-items: center;\">\n<section style=\"width: 8px;height: 8px;background-color: rgb(255, 255, 255);border-radius: 50%;overflow: hidden;\"><br \/><\/section>\n<section data-brushtype=\"text\" style=\"margin-right: 10px;margin-left: 10px;letter-spacing: 1.5px;color: rgb(255, 255, 255);\"><strong>\u5dee\u5f02\u57fa\u56e0\u5206\u6790\u6d41\u7a0b<\/strong><\/section>\n<section style=\"width: 8px;height: 8px;background-color: rgb(255, 255, 255);border-radius: 50%;\"><br \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section>\n<section data-id=\"22363\" powered-by=\"xmyeditor.com\" data-type=\"lspecial02\" data-md5=\"7ab9d\" style=\"font-size: 16px;\">\n<section style=\"text-align: left;\" powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\">\n<section style=\"display: inline-block;vertical-align: middle;border-top: 1px dotted rgb(61, 170, 214);border-bottom: 1px dotted rgb(61, 170, 214);padding-right: 10px;padding-left: 10px;\" powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\">\n<section style=\"color: rgb(61, 170, 214);letter-spacing: 1px;line-height: 24px;text-align: justify;\" powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\">\n<p><strong><span style=\"color: rgb(0, 122, 170);\">1\u3001R\u5305\u4e0e\u6570\u636e\u52a0\u8f7d<\/span><\/strong><strong style=\"color: rgb(0, 122, 170);\"><\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section>\n<section>\n<section>\n<section>\n<section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;letter-spacing: 0.578px;\">\u9996\u5148\uff0c\u8bbe\u7f6e\u5de5\u4f5c\u76ee\u5f55\u4e3a\u6570\u636e\u6587\u4ef6\u6240\u5728\u7684\u8def\u5f84\uff0c\u5e76\u52a0\u8f7dDESeq2\u548c\u5176\u4ed6\u6240\u9700\u7684R\u5305\uff0c\u5e76\u5bfc\u5165\u6211\u4eec\u51c6\u5907\u597d\u7684counts\u8868\u8fbe\u77e9\u9635\uff1a<\/span><\/p>\n<section style=\"margin-bottom: 0px;padding-right: 15px;padding-left: 15px;letter-spacing: 0.578px;text-wrap: wrap;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;background-color: rgb(255, 255, 255);font-size: 14px;visibility: visible;\">\n<section style=\"padding-right: 15px;padding-left: 15px;outline: 0px;visibility: visible;\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cpp\"><code><span class=\"code-snippet_outer\">setwd(\"\/media\/desk16\/iyun007\/kegg\")<\/span><\/code><code><span class=\"code-snippet_outer\">#BiocManager::install(\"DESeq2\") <\/span><\/code><code><span class=\"code-snippet_outer\">library(DESeq2)<\/span><\/code><code><span class=\"code-snippet_outer\">data1 <- read.csv(\"Gene Symbol_matrix.csv\",header = T, row.names = 1)<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<\/section>\n<p><span style=\"font-family: "Open Sans";color: rgb(51, 51, 51);font-size: 14px;\">\u6ce8\u610f\uff0c\u6211\u4eec\u5bfc\u5165\u7684\u6570\u636e\u57fa\u672c\u683c\u5f0f\u5982\u4e0b\uff0c\u540c\u5b66\u4eec\u8981\u63d0\u4f9b\u7b26\u5408\u8981\u6c42\u7684\u6570\u636e\u96c6\u54e6\uff01<\/span><span style=\"mso-spacerun:'yes';font-family:'Open Sans';mso-fareast-font-family:\u5b8b\u4f53;color:rgb(51,51,51);font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section style=\"margin-bottom: 0px;padding-right: 15px;padding-left: 15px;letter-spacing: 0.578px;text-wrap: wrap;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;background-color: rgb(255, 255, 255);font-size: 14px;visibility: visible;\">\n<section style=\"padding-right: 15px;padding-left: 15px;outline: 0px;visibility: visible;\">\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.797752808988764\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_a53fSTBsAicA0b9ZdRRQjQpKnF4COQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwx6zS7gxC4A1mawj0UyqM5cBKU6j3mtiaRc0YKVaDSopKomBia53fSTBsAicA0b9ZdRRQjQpKnF4COQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"623\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<p><span style=\"display: none;line-height: 0px;\">\u200d<\/span><\/p>\n<p style=\"margin-bottom: 0px;text-align: center;\"><br \/><\/p>\n<\/section>\n<\/section>\n<p><span style=\"display: none;line-height: 0px;\">\u200d<\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<section data-id=\"22363\" powered-by=\"xmyeditor.com\" data-type=\"lspecial02\" data-md5=\"7ab9d\" style=\"margin-bottom: 0px;font-size: 16px;\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"text-align: left;\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"padding-right: 10px;padding-left: 10px;display: inline-block;vertical-align: middle;border-top: 1px dotted rgb(61, 170, 214);border-bottom: 1px dotted rgb(61, 170, 214);\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"color: rgb(61, 170, 214);letter-spacing: 1px;line-height: 24px;text-align: justify;\">\n<p><strong><span style=\"color: rgb(0, 122, 170);\">2\u3001\u6570\u636e\u9884\u5904\u7406<\/span><\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;padding-right: 15px;padding-left: 15px;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 0.578px;text-wrap: wrap;background-color: rgb(255, 255, 255);font-size: 14px;visibility: visible;\">\n<section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u8981\u6784\u5efa\u5206\u7ec4\u77e9\u9635\uff0c\u5373\u6839\u636e\u6570\u636e\u6846\u4e2d\u6837\u672c\u7684\u547d\u540d\u89c4\u5219\uff0c\u63d0\u53d6\u51fa\u764c\u75c7\u6837\u672c\u548c\u6b63\u5e38\u6837\u672c\u7684\u5217\u540d\uff0c\u5e76\u5c06\u5b83\u4eec\u5206\u522b\u5b58\u50a8\u5728tumor\u548cnormal\u5411\u91cf\u4e2d\uff1a<\/span><\/p>\n<section style=\"margin-bottom: 0px;padding-right: 15px;padding-left: 15px;letter-spacing: 0.578px;text-wrap: wrap;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;background-color: rgb(255, 255, 255);font-size: 14px;visibility: visible;\">\n<section style=\"padding-right: 15px;padding-left: 15px;outline: 0px;visibility: visible;\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"powershell\"><code><span class=\"code-snippet_outer\">#\u6784\u5efa\u5206\u7ec4\u77e9\u9635<\/span><\/code><code><span class=\"code-snippet_outer\">tumor <- colnames(data1)[as.integer(substr(colnames(data1),14,15)) < 10] #data1\u8868\u8fbe\u77e9\u9635\u4e2d\u7684\u6240\u6709\u764c\u75c7\u6837\u672c\uff088\u4e2a\uff09<\/span><\/code><code><span class=\"code-snippet_outer\">tumor<\/span><\/code><code><span class=\"code-snippet_outer\">normal <- colnames(data1)[as.integer(substr(colnames(data1),14,15)) >= 10] #data1\u8868\u8fbe\u77e9\u9635\u4e2d\u7684\u6240\u6709\u6b63\u5e38\u6837\u672c\uff082\u4e2a\uff09<\/span><\/code><code><span class=\"code-snippet_outer\">normal<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">tumor_sample <- data1[,tumor] #\u764c\u75c7\u6837\u672c\u7684\u8868\u8fbe\u77e9\u9635 <\/span><\/code><code><span class=\"code-snippet_outer\">View(tumor_sample)<\/span><\/code><code><span class=\"code-snippet_outer\">tumor_sample<\/span><\/code><code><span class=\"code-snippet_outer\">ncol(tumor_sample)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">normal_sample <- data1[,normal] #\u6b63\u5e38\u6837\u672c\u7684\u8868\u8fbe\u77e9\u9635 <\/span><\/code><code><span class=\"code-snippet_outer\">View(normal_sample)<\/span><\/code><code><span class=\"code-snippet_outer\">ncol(normal_sample)<\/span><\/code><code><span class=\"code-snippet_outer\">data1 <- cbind(tumor_sample,normal_sample) #\u5408\u5e76\u764c\u75c7\u548c\u6b63\u5e38\u6837\u672c\u77e9\u9635<\/span><\/code><code><span class=\"code-snippet_outer\">col_index <- which(colnames(matrix) == \"normal_sample\")<\/span><\/code><code><span class=\"code-snippet_outer\">colnames(matrix)[col_index] <- normal<\/span><\/code><code><span class=\"code-snippet_outer\">#colnames(data1)[5] <- \"TCGA.AA.3525.11A.01R.A32Z.07\"<\/span><\/code><code><span class=\"code-snippet_outer\">View(data1)<\/span><\/code><code><span class=\"code-snippet_outer\">group_list <- c(rep('tumor',ncol(tumor_sample)),rep('normal',1)) #\u6839\u636e\u81ea\u5df1\u7684\u6570\u636e\u96c6\u8bbe\u7f6etumor\u548cnormal\u6570\u636e\u7684\u5206\u7ec4\u6570<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">condition = factor(group_list)<\/span><\/code><code><span class=\"code-snippet_outer\">coldata <- data.frame(row.names = colnames(data1), condition) #coldata\u662f\u5206\u7ec4\u77e9\u9635<\/span><\/code><code><span class=\"code-snippet_outer\">View(coldata)<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<\/section>\n<p><o:p><\/o:p><\/p>\n<p>\u6700\u540e\u5408\u5e76\u597d\u7684\u6570\u636e\u5982\u4e0b\uff1a<o:p><\/o:p><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.35353535353535354\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u5dee\u5f02\u5206\u6790\u9b54\u6cd5\u5e08\uff1aDESeq2\u4e0e\u57fa\u56e0\u7684\u9b54\u5e7b\u5192\u9669\uff01\/3_RFOFo00vWeUfiaEQl4ib5sU3xMsM3w.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwx6zS7gxC4A1mawj0UyqM5C10S6BFvU2LlAGOhPcJVpo0icRURFOFo00vWeUfiaEQl4ib5sU3xMsM3w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"693\" style=\"\" \/><\/p>\n<p>\u5206\u7ec4\u4fe1\u606f\u5982\u4e0b\uff1a<span style=\"mso-spacerun:'yes';font-family:'Open Sans';mso-fareast-font-family:\u5b8b\u4f53;color:rgb(51,51,51);font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.56353591160221\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_c1U2NkGaPeopBBaEqF45ueULc4Au8Q.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwx6zS7gxC4A1mawj0UyqM56UdylZRZ1TrFUFDLwsht3RyIc1U2NkGaPeopBBaEqF45ueULc4Au8Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"362\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<section style=\"margin-bottom: 0px;\">\n<section>\n<section data-id=\"22363\" powered-by=\"xmyeditor.com\" data-type=\"lspecial02\" data-md5=\"7ab9d\" style=\"font-size: 16px;\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"text-align: left;\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"padding-right: 10px;padding-left: 10px;display: inline-block;vertical-align: middle;border-top: 1px dotted rgb(61, 170, 214);border-bottom: 1px dotted rgb(61, 170, 214);\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"color: rgb(61, 170, 214);letter-spacing: 1px;line-height: 24px;text-align: justify;\">\n<p><strong><span style=\"color: rgb(0, 122, 170);\">3\u3001\u5dee\u5f02\u5206\u6790<\/span><\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;padding-right: 15px;padding-left: 15px;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 0.578px;text-wrap: wrap;background-color: rgb(255, 255, 255);font-size: 14px;visibility: visible;\">\n<section>\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;padding-right: 15px;padding-left: 15px;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 0.578px;text-wrap: wrap;background-color: rgb(255, 255, 255);font-size: 14px;visibility: visible;\">\n<section>\n<p><span style=\"font-size: 14px;\">\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u53ef\u4ee5\uff1a<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">1.\u521b\u5efaDESeq2\u6570\u636e\u5bf9\u8c61\uff1a\u4f7f\u7528DESeqDataSetFromMatrix()\u51fd\u6570\u521b\u5efaDESeq2\u6240\u9700\u7684\u6570\u636e\u5bf9\u8c61\uff0c\u5c06\u5408\u5e76\u540e\u7684\u6570\u636e\u6846\u548c\u5206\u7ec4\u77e9\u9635\u4f5c\u4e3a\u53c2\u6570\u4f20\u9012\u7ed9\u8be5\u51fd\u6570\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">2.\u8fdb\u884c\u5dee\u5f02\u5206\u6790\uff1a\u4f7f\u7528DESeq()\u51fd\u6570\u5bf9DESeq2\u6570\u636e\u5bf9\u8c61\u8fdb\u884c\u5dee\u5f02\u5206\u6790\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">3.\u4fdd\u5b58\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7ed3\u679c\uff1a\u5c06\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7ed3\u679c\u4fdd\u5b58\u4e3aCSV\u6587\u4ef6\u3002<\/span><o:p><\/o:p><\/p>\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;padding-right: 15px;padding-left: 15px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.578px;text-wrap: wrap;outline: 0px;background-color: rgb(255, 255, 255);visibility: visible;\">\n<section style=\"padding-right: 15px;padding-left: 15px;outline: 0px;visibility: visible;\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6784\u5efadds\u5bf9\u8c61\uff0c\u6784\u5efa\u5dee\u5f02\u57fa\u56e0\u5206\u6790\u6240\u9700\u7684\u6570\u636e\u683c\u5f0f<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">dds <- DESeqDataSetFromMatrix(countData = data1, colData = coldata, design = ~ condition)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5dee\u5f02\u5206\u6790<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">dds <- DESe<span class=\"code-snippet__string\">q(dds)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u63d0\u53d6\u7ed3\u679c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">result <- as.data.frame(results(dds)) <span class=\"code-snippet__comment\"># results()\u4eceDESeq\u5206\u6790\u4e2d\u63d0\u53d6\u51fa\u7ed3\u679c\u8868<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">DGE <-subset(result,padj < <span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">05<\/span> & (log2FoldChange > <span class=\"code-snippet__number\">2<\/span> | log2FoldChange < -<span class=\"code-snippet__number\">2<\/span>)) <span class=\"code-snippet__comment\">#\u8fd9\u91cc\u6211\u4eec\u63d0\u53d6\u51fa2\u500d\u5dee\u5f02\u8868\u8fbe\u3001\u6821\u6b63p\u503c<0.05\u7684\u663e\u8457\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">DGE <- DGE[order(DGE$log2FoldChange),]<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">write.csv(DGE,<span class=\"code-snippet__string\">'DGE.csv'<\/span>,row.names = TRUE)<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<\/section>\n<p>\u6211\u4eec\u4e00\u8d77\u6765\u770b\u4e0b\u5206\u6790\u540e\u7684\u7ed3\u679c\u5427\uff01<span style=\"mso-spacerun:'yes';font-family:'Open Sans';mso-fareast-font-family:\u5b8b\u4f53;color:rgb(51,51,51);font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9307359307359307\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u5dee\u5f02\u5206\u6790\u9b54\u6cd5\u5e08\uff1aDESeq2\u4e0e\u57fa\u56e0\u7684\u9b54\u5e7b\u5192\u9669\uff01\/5_XLibVVzQX7uJeSIxgwyDDCCLDbry4w.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwx6zS7gxC4A1mawj0UyqM5JTiau35GgXyL4uKQoaiat5XCgqBXLibVVzQX7uJeSIxgwyDDCCLDbry4w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"693\" style=\"\" \/><\/p>\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.578px;text-wrap: wrap;background-color: rgb(255, 255, 255);\">\n<section>\n<section data-id=\"22363\" powered-by=\"xmyeditor.com\" data-type=\"lspecial02\" data-md5=\"7ab9d\" style=\"font-size: 16px;\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"text-align: left;\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"padding-right: 10px;padding-left: 10px;display: inline-block;vertical-align: middle;border-top: 1px dotted rgb(61, 170, 214);border-bottom: 1px dotted rgb(61, 170, 214);\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"color: rgb(61, 170, 214);letter-spacing: 1px;line-height: 24px;text-align: justify;\">\n<p><strong><span style=\"color: rgb(0, 122, 170);\">4\u3001\u7ed8\u5236\u706b\u5c71\u56fe<\/span><\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;padding-right: 15px;padding-left: 15px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.578px;text-wrap: wrap;outline: 0px;background-color: rgb(255, 255, 255);visibility: visible;\">\n<section>\n<p><br \/><\/p>\n<p>\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u53ef\u4ee5\u901a\u8fc7\u706b\u5c71\u56fe\u53ef\u89c6\u5316\u6211\u4eec\u7684\u5206\u6790\u7ed3\u679c\uff0c\u5177\u4f53\u64cd\u4f5c\u5982\u4e0b\uff1a<o:p><\/o:p><\/p>\n<p>\u8fd9\u91cc\u5c0f\u679c\u4f7f\u7528ggplot()\u51fd\u6570\u521b\u5efa\u7ed8\u56fe\u5bf9\u8c61\uff0c\u5e76\u8bbe\u7f6e\u6570\u636e\u6e90\u4e3aresult\u6570\u636e\u6846\u3002\u7136\u540e\u4f7f\u7528geom_point()\u51fd\u6570\u7ed8\u5236\u6563\u70b9\u56fe\uff0c\u5176\u4e2dx\u8f74\u4e3alog2FoldChange\uff0cy\u8f74\u4e3a-log10(padj)\uff0c\u5e76\u6839\u636e\u5dee\u5f02\u8868\u8fbe\u7684\u65b9\u5411\u548c\u663e\u8457\u6027\u8fdb\u884c\u989c\u8272\u5206\u7c7b\u3002\u901a\u8fc7\u8c03\u6574\u7ed8\u56fe\u7684\u5404\u79cd\u53c2\u6570\uff0c\u5982\u6807\u9898\u3001\u5750\u6807\u8f74\u6807\u7b7e\u548c\u56fe\u4f8b\uff0c\u53ef\u4ee5\u4f7f\u706b\u5c71\u56fe\u66f4\u5177\u53ef\u8bfb\u6027\u548c\u7f8e\u89c2\u6027\u54e6\u3002 <o:p><\/o:p><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">## 5.\u63d0\u53d6\u7ed3\u679c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">result <- as.data.frame(results(dds)) <span class=\"code-snippet__comment\"># results()\u4eceDESeq\u5206\u6790\u4e2d\u63d0\u53d6\u51fa\u7ed3\u679c\u8868<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">result <- na.omit(result)<\/span><\/code><code><span class=\"code-snippet_outer\">result1 <- result[c(<span class=\"code-snippet__number\">2<\/span>,<span class=\"code-snippet__number\">6<\/span>)]<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">## 6.\u706b\u5c71\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">result1$change = ifelse(result1$padj < <span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">05<\/span> & <span class=\"code-snippet__keyword\">abs<\/span>(result1$log2FoldChange) >= <span class=\"code-snippet__number\">2<\/span>, ifelse(result1$log2FoldChange> <span class=\"code-snippet__number\">2<\/span> ,<span class=\"code-snippet__string\">'Up'<\/span>,<span class=\"code-snippet__string\">'Down'<\/span>),<span class=\"code-snippet__string\">'Stable'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">library(<span class=\"code-snippet__string\">'ggplot2'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">ggplot(result1, aes(<span class=\"code-snippet__keyword\">x<\/span> = log2FoldChange, <span class=\"code-snippet__keyword\">y<\/span> = -log1<span class=\"code-snippet__number\">0<\/span>(padj),colour=change)) +<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_point(alpha=<span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">3<\/span>, size=<span class=\"code-snippet__number\">3.5<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_color_manual(<span class=\"code-snippet__keyword\">values<\/span>=c(<span class=\"code-snippet__string\">\"#546de5\"<\/span>,<span class=\"code-snippet__string\">\"#d2dae2\"<\/span>,<span class=\"code-snippet__string\">\"#ff4757\"<\/span>))+<\/span><\/code><code><span class=\"code-snippet_outer\"> labs(title=<span class=\"code-snippet__string\">'Volcano plot'<\/span>,<span class=\"code-snippet__keyword\">x<\/span>=<span class=\"code-snippet__string\">\"log2(fold change)\"<\/span>,<span class=\"code-snippet__keyword\">y<\/span>=<span class=\"code-snippet__string\">\"-log10 (padj)\"<\/span>)+ <span class=\"code-snippet__comment\"># \u5750\u6807\u8f74# \u5750\u6807\u8f74\u548c\u56fe\u6807\u9898title=\"Volcano plot\",<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> theme_bw()+ <span class=\"code-snippet__comment\">#\u53bb\u9664\u80cc\u666f\u8272<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\">#xlim(-20, 20)+ #\u8bbe\u7f6e\u5750\u6807\u8f74\u8303\u56f4<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\">#ylim(0,40)+<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\"># \u8f85\u52a9\u7ebf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> geom_vline(xintercept=c(-<span class=\"code-snippet__number\">2<\/span>,<span class=\"code-snippet__number\">2<\/span>),lty=<span class=\"code-snippet__number\">3<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">8<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_hline(yintercept = -log1<span class=\"code-snippet__number\">0<\/span>(<span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">05<\/span>),lty=<span class=\"code-snippet__number\">3<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">8<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> theme(panel.grid = element_blank())+ <span class=\"code-snippet__comment\">#\u53bb\u9664\u7f51\u683c\u7ebf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> theme(plot.title = element_text(hjust = <span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">5<\/span>,size=<span class=\"code-snippet__number\">24<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.position=<span class=\"code-snippet__string\">\"bottom\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.title = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.text=element_text(size=<span class=\"code-snippet__number\">18<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.key.size = unit(<span class=\"code-snippet__number\">1<\/span>, <span class=\"code-snippet__string\">'cm'<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.background = element_rect(fill=<span class=\"code-snippet__string\">\"gray90\"<\/span>, linetype=<span class=\"code-snippet__string\">\"solid\"<\/span>,colour =<span class=\"code-snippet__string\">\"gray\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.title.x =element_text(size=<span class=\"code-snippet__number\">18<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.title.y=element_text(size=<span class=\"code-snippet__number\">18<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.text=element_text(size=<span class=\"code-snippet__number\">14<\/span>,face = <span class=\"code-snippet__string\">\"bold\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> )<\/span><\/code><code><span class=\"code-snippet_outer\">ggsave(<span class=\"code-snippet__string\">\"De_ana.png\"<\/span>,width=<span class=\"code-snippet__number\">8<\/span>,height=<span class=\"code-snippet__number\">6<\/span>)<span class=\"code-snippet__comment\"># \u4fdd\u5b58\u4e3apng\u683c\u5f0f<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p>\u6211\u4eec\u4e00\u8d77\u6765\u770b\u4e0b\u6700\u540e\u7684\u7ed3\u679c\u56fe\u5427\uff01<o:p><\/o:p><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.7503607503607503\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_czqOv8XDtgamzict1qib2qe7qxqh2w.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwx6zS7gxC4A1mawj0UyqM5TREZGJoC4GoibR8ib9Q3HgvEw2pniczqOv8XDtgamzict1qib2qe7qxqh2w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"693\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p><br \/><\/p>\n<p>\u600e\u4e48\u6837\uff0c\u4f60\u5b66\u4f1a\u600e\u4e48\u4f7f\u7528DESeq2\u5305\u6765\u8fdb\u884c\u5dee\u5f02\u5206\u6790\u4e86\u561b\uff1f\u662f\u4e0d\u662f\u5f88\u7b80\u5355\uff01\u66f4\u591a\u5b66\u4e60\u5e72\u6d3b\u8981\u7ee7\u7eed\u5173\u6ce8\u5c0f\u679c\u54e6\uff01<o:p><\/o:p><\/p>\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section>\n<p><span style=\"font-size: 14px;color: rgb(0, 122, 170);\">\u8fd9\u91cc\u63a8\u8350\u4e00\u4e0b\u5c0f\u679c\u65b0\u5f00\u53d1\u7684\u96f6\u4ee3\u7801\u4e91\u751f\u4fe1\u5206\u6790\u5de5\u5177\u5e73\u53f0\u5305\u542b\u8d85\u591a\u96f6\u4ee3\u7801\u5c0f\u5de5\u5177\uff0c\u4e0a\u4f20\u6570\u636e\u4e00\u952e\u51fa\u56fe\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u4e86\u89e3~<\/span><span style=\"text-align: center;letter-spacing: 0.578px;font-size: var(--articleFontsize);\"><\/span><\/p>\n<p><span style=\"font-size: 14px;color: rgb(0, 122, 170);\">\u7f51\u5740\uff1ahttp:\/\/www.biocloudservice.com\/home.html<\/span><\/p>\n<p><br \/><\/p>\n<section data-id=\"75061\" data-type=\"lspecial02\" data-tools=\"\u901f\u6392\u5c0f\u8682\u8681\u7f16\u8f91\u5668\" powered-by=\"xmyeditor.com\" data-md5=\"83a15\">\n<p><span style=\"font-size: 14px;\">\u4eca\u5929\u5c0f\u679c\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\uff0c\u6b22\u8fce\u5927\u5bb6\u548c\u5c0f\u679c\u4e00\u8d77\u8ba8\u8bba\u5b66\u4e60\uff0c\u4e0b\u671f\u518d\u89c1\u54e6\uff01<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"padding-right: 15px;padding-left: 15px;outline: 0px;font-size: 14px;\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;height: auto;transform: perspective(0px);transform-style: flat;\">\n<section style=\"margin-top: 10px;margin-bottom: -3px;outline: 0px;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;transform: translate3d(19px, 0px, 0px) rotateX(180deg);\">\n<section style=\"outline: 0px;display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: center;\">\n<section style=\"outline: 0px;display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 230px;background-color: rgb(217, 217, 217);\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: justify;\">\n<p style=\"outline: 0px;\"><br style=\"outline: 0px;\" \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"outline: 0px;display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: center;\">\n<section style=\"outline: 0px;display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 230px;background-color: rgb(217, 217, 217);\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: justify;\">\n<p style=\"outline: 0px;\"><br style=\"outline: 0px;\" \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"outline: 0px;display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: center;\">\n<section style=\"outline: 0px;display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 230px;background-color: rgb(217, 217, 217);\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: justify;\">\n<p style=\"outline: 0px;\"><br style=\"outline: 0px;\" \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 10px;outline: 0px;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"padding: 14px 10px;outline: 0px;display: inline-block;width: 647px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;border-style: solid;border-width: 1px;border-color: rgb(217, 217, 217);\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 15px;outline: 0px;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"padding-left: 9px;outline: 0px;display: inline-block;width: 625px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 0px 12px;border-left-color: rgb(212, 32, 2);\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: justify;font-size: 17px;color: rgb(62, 62, 62);\">\n<p style=\"outline: 0px;\"><strong style=\"outline: 0px;\">\u5c0f\u679c\u53cb\u60c5\u63a8\u8350<\/strong><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 3px;outline: 0px;\">\n<section style=\"outline: 0px;background-color: rgb(217, 217, 217);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: justify;font-size: 11px;color: rgb(62, 62, 62);\">\n<p style=\"outline: 0px;\">\u597d\u7528\u53c8\u514d\u8d39\u7684\u5de5\u5177\u5b89\u5229<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;outline: 0px;text-align: center;line-height: 0;\">\n<section style=\"outline: 0px;vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.35777777777777775\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u5dee\u5f02\u5206\u6790\u9b54\u6cd5\u5e08\uff1aDESeq2\u4e0e\u57fa\u56e0\u7684\u9b54\u5e7b\u5192\u9669\uff01\/7_eVibso6VJox4Hnic0n53VnLS1A9qYA.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAyqVJd7As6iaIgTzXqErqUGpoEpMoWhEkVHHw4z3ZmlYpZZUX7eVibso6VJox4Hnic0n53VnLS1A9qYA\/640?wx_fmt=jpeg&wxfrom=5&wx_lazy=1&wx_co=1'\" data-type=\"jpeg\" data-w=\"900\" style=\"outline: 0px;vertical-align: middle;width: 625px;visibility: visible !important;\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;outline: 0px;text-align: center;line-height: 0;\">\n<section style=\"outline: 0px;vertical-align: middle;display: inline-block;line-height: 0;\"><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650762044&idx=2&sn=c74f81e91cadd166adc7168dec9891ed&scene=21#wechat_redirect\" textvalue=\"\u4f60\u5df2\u9009\u4e2d\u4e86\u6dfb\u52a0\u94fe\u63a5\u7684\u5185\u5bb9\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" 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textvalue=\"\u4f60\u5df2\u9009\u4e2d\u4e86\u6dfb\u52a0\u94fe\u63a5\u7684\u5185\u5bb9\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\" style=\"outline: 0px;line-height: 0;vertical-align: bottom;user-select: none;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.36333333333333334\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u5dee\u5f02\u5206\u6790\u9b54\u6cd5\u5e08\uff1aDESeq2\u4e0e\u57fa\u56e0\u7684\u9b54\u5e7b\u5192\u9669\uff01\/9_IHSYtLyxwVWxiaf2v2rQdOnoz05G6A.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAyqVJd7As6iaIgTzXqErqUGp9ZZibzJmVhqwxDqOgC1Tdmh7VxIHSYtLyxwVWxiaf2v2rQdOnoz05G6A\/640?wx_fmt=jpeg&wxfrom=5&wx_lazy=1&wx_co=1'\" data-type=\"jpeg\" data-w=\"900\" style=\"outline: 0px;border-width: 0px;border-style: initial;border-color: initial;vertical-align: middle;width: 625px;visibility: visible !important;\" \/><\/span><\/a><\/section>\n<p><span style=\"text-align: justify;letter-spacing: 0.5px;text-indent: 0em;color: rgb(0, 150, 136);font-size: 32px;caret-color: rgba(0, 0, 0, 0);\"><\/span><span style=\"text-align: justify;letter-spacing: 0.5px;text-indent: 0em;color: rgb(0, 150, 136);font-size: 32px;caret-color: rgba(0, 0, 0, 0);\"><\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"https:\/\/mp.weixin.qq.com\/s\/sfk0hRc6tkxjkKkvuVHjnA#rd\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"https:\/\/mp.weixin.qq.com\/s\/sfk0hRc6tkxjkKkvuVHjnA#rd\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5dee\u5f02\u5206\u6790\u9b54\u6cd5\u5e08\uff1aDESeq2\u4e0e\u57fa\u56e0\u7684\u9b54\u5e7b\u5192\u9669\uff01 \u539f\u521b \u5c0f\u679c \u751f\u4fe1\u679c 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{"id":54764,"date":"2024-06-16T14:27:28","date_gmt":"2024-06-16T06:27:28","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=54764"},"modified":"2024-06-16T14:27:28","modified_gmt":"2024-06-16T06:27:28","slug":"%e5%b7%ae%e5%bc%82%e5%88%86%e6%9e%90%e9%ad%94%e6%b3%95%e5%b8%88%ef%bc%9adeseq2%e4%b8%8e%e5%9f%ba%e5%9b%a0%e7%9a%84%e9%ad%94%e5%b9%bb%e5%86%92%e9%99%a9%ef%bc%81-2","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=54764","title":{"rendered":"\u5dee\u5f02\u5206\u6790\u9b54\u6cd5\u5e08\uff1aDESeq2\u4e0e\u57fa\u56e0\u7684\u9b54\u5e7b\u5192\u9669\uff01"},"content":{"rendered":"

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class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-08-27 19:00:38<\/em><\/div>\n<p><content><\/p>\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;\" data-mpa-powered-by=\"yiban.io\">\n<section style=\"\">\n<section style=\"\">\n<section style=\"\">\n<section style=\"\">\n<section style=\"\">\n<section data-width=\"100%\" style=\"font-size: 16px;width: 578px;flex: 0 0 100%;opacity: 1;\">\n<section style=\"line-height: 1.8;\">\n<section>\n<section>\n<section style=\"margin-top: 10px;margin-bottom: 20px;display: flex;flex-flow: row nowrap;text-align: center;justify-content: center;\">\n<section style=\"display: inline-block;width: 578px;vertical-align: top;border-style: solid;border-width: 1px;border-color: rgb(67, 67, 67);flex: 0 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style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u751f\u4fe1\u4ebaR\u8bed\u8a00\u5b66\u4e60\u5fc5\u5907<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u7acb\u523b\u62e5\u6709\u4e00\u4e2aRstudio\u8d26\u53f7<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u5f00\u542f\u5347\u7ea7\u6a21\u5f0f\u5427<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"color: rgb(255, 129, 36);font-size: 12px;letter-spacing: 0.5px;\">\uff0856\u7ebf\u7a0b\uff0c256G\u5185\u5b58\uff0c\u4e2a\u4eba\u5b58\u50a81T\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 29.9652%;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 29.9652% !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;line-height: 0;\">\n<section>\n<section style=\"display: inline-block;vertical-align: middle;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 0;\">\n<section style=\"width: 111px;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 1.75em;text-indent: 0em;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;height: auto;width: 111px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u5dee\u5f02\u5206\u6790\u9b54\u6cd5\u5e08\uff1aDESeq2\u4e0e\u57fa\u56e0\u7684\u9b54\u5e7b\u5192\u9669\uff01\/1_GBhsibRAgNiaQsH4ichdP9ibEhHMFA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYngnlT6jiavu08Bvp2iblzGf3IkoBD9NGBhsibRAgNiaQsH4ichdP9ibEhHMFA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"400\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"font-size: var(--articleFontsize);letter-spacing: 0.578px;text-align: justify;\"><\/span><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section data-role=\"list\" style=\"\">\n<section powered-by=\"xiumi.us\" style=\"\">\n<section style=\"\">\n<section powered-by=\"xiumi.us\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 8px;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 0.578px;text-wrap: wrap;background-color: rgb(255, 255, 255);font-size: 16px;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;visibility: visible;\">\n<section style=\"padding-left: 8px;outline: 0px;display: inline-block;width: 578px;vertical-align: top;border-left: 3px solid rgb(219, 219, 219);border-bottom-left-radius: 0px;align-self: flex-start;flex: 0 0 auto;visibility: visible;\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;color: rgba(0, 0, 0, 0.5);text-align: justify;visibility: visible;\">\n<p style=\"outline: 0px;visibility: visible;\"><span style=\"outline: 0px;font-size: 14px;visibility: visible;\">\u8fd8\u5728\u4e3a\u5982\u4f55\u5165\u95e8\u751f\u4fe1\u800c\u5f77\u5fa8\u5417\uff1f\u8fd8\u5728\u4e3aLinux\u7cfb\u7edf\u800c\u611f\u5230\u65e0\u52a9\u5417\uff1f\u5feb\u5173\u6ce8\u5c0f\u679c\u7684\u5fae\u4fe1\u516c\u4f17\u53f7\uff0c\u5c0f\u679c\u624b\u628a\u624b\u5e26\u4f60\u5165\u95e8\u751f\u4fe1\uff0c\u6ca1\u6709\u95e8\u69db\u3001\u6ca1\u6709\u96be\u5ea6\uff0c\u8ddf\u7740\u6548\u679c\u8d70\uff0c\u5565\u95ee\u9898\u90fd\u6ca1\u6709\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<p><br \/><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14px;color: rgb(51, 51, 51);font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">hello<span style=\"color: rgb(51, 51, 51);\">\uff0c\u4eca\u5929\u5c0f\u679c\u6559\u5927\u5bb6\u5982\u4f55\u4f7f\u7528DESeq2\u5305\u5bf9counts\u8868\u8fbe\u77e9\u9635\u8fdb\u884c\u5dee\u5f02\u57fa\u56e0\u5206\u6790\uff0c\u5e76\u5bf9\u5206\u6790\u7ed3\u679c\u7ed8\u5236\u706b\u5c71\u56fe\u5b9e\u73b0\u5dee\u5f02\u57fa\u56e0\u5206\u6790\u7684\u53ef\u89c6\u5316\uff0c\u5982\u679c\u4f60\u611f\u5174\u8da3\u7684\u8bdd\u5c31\u548c\u5c0f\u679c\u4e00\u8d77\u770b\u4e0b\u53bb\u5427\uff01<\/span><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"color: rgb(51, 51, 51);font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u9996\u5148\u5c0f\u679c\u5148\u6765\u7b80\u5355\u4ecb\u7ecd\u4e00\u4e0b\u8fd9\u4e2a\u5305\uff1a<\/span><span style=\"mso-spacerun:'yes';font-family:'Open Sans';mso-fareast-font-family:\u5b8b\u4f53;color:rgb(51,51,51);font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<section data-role=\"title\" data-tools=\"135\u7f16\u8f91\u5668\" data-id=\"104726\" style=\"margin-bottom: 0px;font-size: 16px;letter-spacing: 0.578px;text-wrap: wrap;\">\n<section style=\"margin: 10px auto;text-align: center;\">\n<section style=\"display: inline-block;\">\n<section style=\"padding: 7px 10px;background-color: rgb(84, 141, 212);\">\n<section style=\"display: flex;justify-content: center;align-items: center;\">\n<section style=\"width: 8px;height: 8px;background-color: rgb(255, 255, 255);border-radius: 50%;overflow: hidden;\"><br \/><\/section>\n<section data-brushtype=\"text\" style=\"margin-right: 10px;margin-left: 10px;letter-spacing: 1.5px;color: rgb(255, 255, 255);\"><strong>\u4e86\u89e3DESeq2<\/strong><\/section>\n<section style=\"width: 8px;height: 8px;background-color: rgb(255, 255, 255);border-radius: 50%;\"><br \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p><span style=\"font-family: "Open Sans";color: rgb(51, 51, 51);font-size: 14px;\">DESeq2<span style=\"color: rgb(51, 51, 51);font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u662f\u4e00\u4e2aR\u8bed\u8a00\u4e2d\u5e38\u7528\u7684\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u5305\uff0c\u7528\u4e8e\u5206\u6790\u9ad8\u901a\u91cf\u6d4b\u5e8f\u6570\u636e\u4e2d\u7684\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u3002\u5b83\u662fBioconductor\u9879\u76ee\u7684\u4e00\u90e8\u5206\uff0c\u63d0\u4f9b\u4e86\u4e00\u5957\u7edf\u8ba1\u65b9\u6cd5\u548c\u529f\u80fd\uff0c\u7528\u4e8e\u68c0\u6d4b\u57fa\u56e0\u5728\u4e0d\u540c\u6761\u4ef6\u4e0b\u7684\u8868\u8fbe\u5dee\u5f02\uff0c\u5e76\u627e\u51fa\u5177\u6709\u751f\u7269\u5b66\u610f\u4e49\u7684\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u3002DESeq2\u5305\u7684\u8bbe\u8ba1\u76ee\u6807\u662f\u9488\u5bf9RNA-seq\u6570\u636e\u8fdb\u884c\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff0c\u5e76\u4e14\u80fd\u591f\u5904\u7406\u5177\u6709\u4f4e\u590d\u5236\u6570\u3001\u9ad8\u53d8\u5f02\u6027\u548c\u6709\u504f\u5dee\u7684\u6570\u636e\u3002\u5b83\u91c7\u7528\u4e86\u8d1f\u4e8c\u9879\u5206\u5e03\u6a21\u578b\u548c\u5e7f\u4e49\u7ebf\u6027\u6a21\u578b\uff08GLM<\/span><span style=\"color: rgb(51, 51, 51);font-family: \u5b8b\u4f53;\">\uff09\u7684\u65b9\u6cd5\uff0c\u901a\u8fc7\u8003\u8651\u6837\u672c\u4e4b\u95f4\u7684\u6280\u672f\u548c\u751f\u7269\u5b66\u53d8\u5f02\uff0c\u8fdb\u884c\u51c6\u786e\u7684\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u3002<\/span><o:p><\/o:p><\/span><\/p>\n<p style=\"font-size: 16px;\"><br \/><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">DESeq2\u5305\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u529f\u80fd\uff0c\u5305\u62ec\uff1a<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;color: rgb(0, 122, 170);\">1.\u6570\u636e\u5bfc\u5165<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\uff1a\u652f\u6301\u4ece\u4e0d\u540c\u7684\u6570\u636e\u6e90\u5bfc\u5165RNA-seq\u6570\u636e\uff0c\u5305\u62ec\u539f\u59cb\u8ba1\u6570\u6570\u636e\u548c\u5df2\u7ecf\u8fdb\u884c\u4e86\u57fa\u56e0\u6ce8\u91ca\u7684\u6570\u636e\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;color: rgb(0, 122, 170);\">2.\u6570\u636e\u9884\u5904\u7406<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\uff1a\u5305\u62ec\u6570\u636e\u89c4\u8303\u5316\u3001\u53bb\u9664\u4f4e\u8868\u8fbe\u57fa\u56e0\u3001\u53bb\u9664\u6279\u6b21\u6548\u5e94\u7b49\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;color: rgb(0, 122, 170);\">3.\u5dee\u5f02\u8868\u8fbe\u5206\u6790<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\uff1a\u4f7f\u7528\u8d1f\u4e8c\u9879\u5206\u5e03\u6a21\u578b\u548cGLM\u8fdb\u884c\u7edf\u8ba1\u5206\u6790\uff0c\u8ba1\u7b97\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u663e\u8457\u6027\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;color: rgb(0, 122, 170);\">4.\u591a\u7ec4\u5b9e\u9a8c\u8bbe\u8ba1<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\uff1a\u652f\u6301\u591a\u7ec4\u6761\u4ef6\u4e0b\u7684\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff0c\u5305\u62ec\u4e24\u7ec4\u6bd4\u8f83\u3001\u591a\u7ec4\u6bd4\u8f83\u548c\u65f6\u95f4\u5e8f\u5217\u5206\u6790\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;color: rgb(0, 122, 170);\"><strong><span style=\"text-decoration: underline;font-size: 14px;\">5.\u57fa\u56e0\u6ce8\u91ca\u548c\u529f\u80fd\u5bcc\u96c6\u5206\u6790<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\uff1a\u652f\u6301\u5c06\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u8fdb\u884c\u6ce8\u91ca\uff0c\u5e76\u8fdb\u884c\u529f\u80fd\u5bcc\u96c6\u5206\u6790\uff0c\u4ee5\u4e86\u89e3\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u751f\u7269\u5b66\u529f\u80fd\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;color: rgb(0, 122, 170);\">6.\u53ef\u89c6\u5316<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\uff1aDESeq2\u5305\u96c6\u6210\u4e86ggplot2\u548c\u5176\u4ed6\u53ef\u89c6\u5316\u5de5\u5177\uff0c\u53ef\u4ee5\u65b9\u4fbf\u5730\u7ed8\u5236\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u56fe\u8868\uff0c\u5982\u706b\u5c71\u56fe\u3001MA\u56fe\u7b49\u3002<\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:'Open Sans';mso-fareast-font-family:\u5b8b\u4f53;color:rgb(51,51,51);font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><br \/><\/o:p><\/span><\/p>\n<section data-role=\"title\" data-tools=\"135\u7f16\u8f91\u5668\" data-id=\"104726\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;text-wrap: wrap;font-size: 16px;\">\n<section style=\"margin: 10px auto;text-align: center;\">\n<section style=\"display: inline-block;\">\n<section style=\"padding: 7px 10px;background-color: rgb(84, 141, 212);\">\n<section style=\"display: flex;justify-content: center;align-items: center;\">\n<section style=\"width: 8px;height: 8px;background-color: rgb(255, 255, 255);border-radius: 50%;overflow: hidden;\"><br \/><\/section>\n<section data-brushtype=\"text\" style=\"margin-right: 10px;margin-left: 10px;letter-spacing: 1.5px;color: rgb(255, 255, 255);\"><strong>\u5dee\u5f02\u57fa\u56e0\u5206\u6790\u6d41\u7a0b<\/strong><\/section>\n<section style=\"width: 8px;height: 8px;background-color: rgb(255, 255, 255);border-radius: 50%;\"><br \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section>\n<section data-id=\"22363\" powered-by=\"xmyeditor.com\" data-type=\"lspecial02\" data-md5=\"7ab9d\" style=\"font-size: 16px;\">\n<section style=\"text-align: left;\" powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\">\n<section style=\"display: inline-block;vertical-align: middle;border-top: 1px dotted rgb(61, 170, 214);border-bottom: 1px dotted rgb(61, 170, 214);padding-right: 10px;padding-left: 10px;\" powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\">\n<section style=\"color: rgb(61, 170, 214);letter-spacing: 1px;line-height: 24px;text-align: justify;\" powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\">\n<p><strong><span style=\"color: rgb(0, 122, 170);\">1\u3001R\u5305\u4e0e\u6570\u636e\u52a0\u8f7d<\/span><\/strong><strong style=\"color: rgb(0, 122, 170);\"><\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section>\n<section>\n<section>\n<section>\n<section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;letter-spacing: 0.578px;\">\u9996\u5148\uff0c\u8bbe\u7f6e\u5de5\u4f5c\u76ee\u5f55\u4e3a\u6570\u636e\u6587\u4ef6\u6240\u5728\u7684\u8def\u5f84\uff0c\u5e76\u52a0\u8f7dDESeq2\u548c\u5176\u4ed6\u6240\u9700\u7684R\u5305\uff0c\u5e76\u5bfc\u5165\u6211\u4eec\u51c6\u5907\u597d\u7684counts\u8868\u8fbe\u77e9\u9635\uff1a<\/span><\/p>\n<section style=\"margin-bottom: 0px;padding-right: 15px;padding-left: 15px;letter-spacing: 0.578px;text-wrap: wrap;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;background-color: rgb(255, 255, 255);font-size: 14px;visibility: visible;\">\n<section style=\"padding-right: 15px;padding-left: 15px;outline: 0px;visibility: visible;\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cpp\"><code><span class=\"code-snippet_outer\">setwd(\"\/media\/desk16\/iyun007\/kegg\")<\/span><\/code><code><span class=\"code-snippet_outer\">#BiocManager::install(\"DESeq2\") <\/span><\/code><code><span class=\"code-snippet_outer\">library(DESeq2)<\/span><\/code><code><span class=\"code-snippet_outer\">data1 <- read.csv(\"Gene Symbol_matrix.csv\",header = T, row.names = 1)<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<\/section>\n<p><span style=\"font-family: "Open Sans";color: rgb(51, 51, 51);font-size: 14px;\">\u6ce8\u610f\uff0c\u6211\u4eec\u5bfc\u5165\u7684\u6570\u636e\u57fa\u672c\u683c\u5f0f\u5982\u4e0b\uff0c\u540c\u5b66\u4eec\u8981\u63d0\u4f9b\u7b26\u5408\u8981\u6c42\u7684\u6570\u636e\u96c6\u54e6\uff01<\/span><span style=\"mso-spacerun:'yes';font-family:'Open Sans';mso-fareast-font-family:\u5b8b\u4f53;color:rgb(51,51,51);font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section style=\"margin-bottom: 0px;padding-right: 15px;padding-left: 15px;letter-spacing: 0.578px;text-wrap: wrap;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;background-color: rgb(255, 255, 255);font-size: 14px;visibility: visible;\">\n<section style=\"padding-right: 15px;padding-left: 15px;outline: 0px;visibility: visible;\">\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.797752808988764\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_a53fSTBsAicA0b9ZdRRQjQpKnF4COQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwx6zS7gxC4A1mawj0UyqM5cBKU6j3mtiaRc0YKVaDSopKomBia53fSTBsAicA0b9ZdRRQjQpKnF4COQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"623\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<p><span style=\"display: none;line-height: 0px;\">\u200d<\/span><\/p>\n<p style=\"margin-bottom: 0px;text-align: center;\"><br \/><\/p>\n<\/section>\n<\/section>\n<p><span style=\"display: none;line-height: 0px;\">\u200d<\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<section data-id=\"22363\" powered-by=\"xmyeditor.com\" data-type=\"lspecial02\" data-md5=\"7ab9d\" style=\"margin-bottom: 0px;font-size: 16px;\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"text-align: left;\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"padding-right: 10px;padding-left: 10px;display: inline-block;vertical-align: middle;border-top: 1px dotted rgb(61, 170, 214);border-bottom: 1px dotted rgb(61, 170, 214);\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"color: rgb(61, 170, 214);letter-spacing: 1px;line-height: 24px;text-align: justify;\">\n<p><strong><span style=\"color: rgb(0, 122, 170);\">2\u3001\u6570\u636e\u9884\u5904\u7406<\/span><\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;padding-right: 15px;padding-left: 15px;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 0.578px;text-wrap: wrap;background-color: rgb(255, 255, 255);font-size: 14px;visibility: visible;\">\n<section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u8981\u6784\u5efa\u5206\u7ec4\u77e9\u9635\uff0c\u5373\u6839\u636e\u6570\u636e\u6846\u4e2d\u6837\u672c\u7684\u547d\u540d\u89c4\u5219\uff0c\u63d0\u53d6\u51fa\u764c\u75c7\u6837\u672c\u548c\u6b63\u5e38\u6837\u672c\u7684\u5217\u540d\uff0c\u5e76\u5c06\u5b83\u4eec\u5206\u522b\u5b58\u50a8\u5728tumor\u548cnormal\u5411\u91cf\u4e2d\uff1a<\/span><\/p>\n<section style=\"margin-bottom: 0px;padding-right: 15px;padding-left: 15px;letter-spacing: 0.578px;text-wrap: wrap;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;background-color: rgb(255, 255, 255);font-size: 14px;visibility: visible;\">\n<section style=\"padding-right: 15px;padding-left: 15px;outline: 0px;visibility: visible;\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"powershell\"><code><span class=\"code-snippet_outer\">#\u6784\u5efa\u5206\u7ec4\u77e9\u9635<\/span><\/code><code><span class=\"code-snippet_outer\">tumor <- colnames(data1)[as.integer(substr(colnames(data1),14,15)) < 10] #data1\u8868\u8fbe\u77e9\u9635\u4e2d\u7684\u6240\u6709\u764c\u75c7\u6837\u672c\uff088\u4e2a\uff09<\/span><\/code><code><span class=\"code-snippet_outer\">tumor<\/span><\/code><code><span class=\"code-snippet_outer\">normal <- colnames(data1)[as.integer(substr(colnames(data1),14,15)) >= 10] #data1\u8868\u8fbe\u77e9\u9635\u4e2d\u7684\u6240\u6709\u6b63\u5e38\u6837\u672c\uff082\u4e2a\uff09<\/span><\/code><code><span class=\"code-snippet_outer\">normal<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">tumor_sample <- data1[,tumor] #\u764c\u75c7\u6837\u672c\u7684\u8868\u8fbe\u77e9\u9635 <\/span><\/code><code><span class=\"code-snippet_outer\">View(tumor_sample)<\/span><\/code><code><span class=\"code-snippet_outer\">tumor_sample<\/span><\/code><code><span class=\"code-snippet_outer\">ncol(tumor_sample)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">normal_sample <- data1[,normal] #\u6b63\u5e38\u6837\u672c\u7684\u8868\u8fbe\u77e9\u9635 <\/span><\/code><code><span class=\"code-snippet_outer\">View(normal_sample)<\/span><\/code><code><span class=\"code-snippet_outer\">ncol(normal_sample)<\/span><\/code><code><span class=\"code-snippet_outer\">data1 <- cbind(tumor_sample,normal_sample) #\u5408\u5e76\u764c\u75c7\u548c\u6b63\u5e38\u6837\u672c\u77e9\u9635<\/span><\/code><code><span class=\"code-snippet_outer\">col_index <- which(colnames(matrix) == \"normal_sample\")<\/span><\/code><code><span class=\"code-snippet_outer\">colnames(matrix)[col_index] <- normal<\/span><\/code><code><span class=\"code-snippet_outer\">#colnames(data1)[5] <- \"TCGA.AA.3525.11A.01R.A32Z.07\"<\/span><\/code><code><span class=\"code-snippet_outer\">View(data1)<\/span><\/code><code><span class=\"code-snippet_outer\">group_list <- c(rep('tumor',ncol(tumor_sample)),rep('normal',1)) #\u6839\u636e\u81ea\u5df1\u7684\u6570\u636e\u96c6\u8bbe\u7f6etumor\u548cnormal\u6570\u636e\u7684\u5206\u7ec4\u6570<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">condition = factor(group_list)<\/span><\/code><code><span class=\"code-snippet_outer\">coldata <- data.frame(row.names = colnames(data1), condition) #coldata\u662f\u5206\u7ec4\u77e9\u9635<\/span><\/code><code><span class=\"code-snippet_outer\">View(coldata)<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<\/section>\n<p><o:p><\/o:p><\/p>\n<p>\u6700\u540e\u5408\u5e76\u597d\u7684\u6570\u636e\u5982\u4e0b\uff1a<o:p><\/o:p><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.35353535353535354\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u5dee\u5f02\u5206\u6790\u9b54\u6cd5\u5e08\uff1aDESeq2\u4e0e\u57fa\u56e0\u7684\u9b54\u5e7b\u5192\u9669\uff01\/3_RFOFo00vWeUfiaEQl4ib5sU3xMsM3w.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwx6zS7gxC4A1mawj0UyqM5C10S6BFvU2LlAGOhPcJVpo0icRURFOFo00vWeUfiaEQl4ib5sU3xMsM3w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"693\" style=\"\" \/><\/p>\n<p>\u5206\u7ec4\u4fe1\u606f\u5982\u4e0b\uff1a<span style=\"mso-spacerun:'yes';font-family:'Open Sans';mso-fareast-font-family:\u5b8b\u4f53;color:rgb(51,51,51);font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.56353591160221\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_c1U2NkGaPeopBBaEqF45ueULc4Au8Q.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwx6zS7gxC4A1mawj0UyqM56UdylZRZ1TrFUFDLwsht3RyIc1U2NkGaPeopBBaEqF45ueULc4Au8Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"362\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<section style=\"margin-bottom: 0px;\">\n<section>\n<section data-id=\"22363\" powered-by=\"xmyeditor.com\" data-type=\"lspecial02\" data-md5=\"7ab9d\" style=\"font-size: 16px;\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"text-align: left;\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"padding-right: 10px;padding-left: 10px;display: inline-block;vertical-align: middle;border-top: 1px dotted rgb(61, 170, 214);border-bottom: 1px dotted rgb(61, 170, 214);\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"color: rgb(61, 170, 214);letter-spacing: 1px;line-height: 24px;text-align: justify;\">\n<p><strong><span style=\"color: rgb(0, 122, 170);\">3\u3001\u5dee\u5f02\u5206\u6790<\/span><\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;padding-right: 15px;padding-left: 15px;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 0.578px;text-wrap: wrap;background-color: rgb(255, 255, 255);font-size: 14px;visibility: visible;\">\n<section>\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;padding-right: 15px;padding-left: 15px;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 0.578px;text-wrap: wrap;background-color: rgb(255, 255, 255);font-size: 14px;visibility: visible;\">\n<section>\n<p><span style=\"font-size: 14px;\">\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u53ef\u4ee5\uff1a<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">1.\u521b\u5efaDESeq2\u6570\u636e\u5bf9\u8c61\uff1a\u4f7f\u7528DESeqDataSetFromMatrix()\u51fd\u6570\u521b\u5efaDESeq2\u6240\u9700\u7684\u6570\u636e\u5bf9\u8c61\uff0c\u5c06\u5408\u5e76\u540e\u7684\u6570\u636e\u6846\u548c\u5206\u7ec4\u77e9\u9635\u4f5c\u4e3a\u53c2\u6570\u4f20\u9012\u7ed9\u8be5\u51fd\u6570\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">2.\u8fdb\u884c\u5dee\u5f02\u5206\u6790\uff1a\u4f7f\u7528DESeq()\u51fd\u6570\u5bf9DESeq2\u6570\u636e\u5bf9\u8c61\u8fdb\u884c\u5dee\u5f02\u5206\u6790\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">3.\u4fdd\u5b58\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7ed3\u679c\uff1a\u5c06\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7ed3\u679c\u4fdd\u5b58\u4e3aCSV\u6587\u4ef6\u3002<\/span><o:p><\/o:p><\/p>\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;padding-right: 15px;padding-left: 15px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.578px;text-wrap: wrap;outline: 0px;background-color: rgb(255, 255, 255);visibility: visible;\">\n<section style=\"padding-right: 15px;padding-left: 15px;outline: 0px;visibility: visible;\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6784\u5efadds\u5bf9\u8c61\uff0c\u6784\u5efa\u5dee\u5f02\u57fa\u56e0\u5206\u6790\u6240\u9700\u7684\u6570\u636e\u683c\u5f0f<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">dds <- DESeqDataSetFromMatrix(countData = data1, colData = coldata, design = ~ condition)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5dee\u5f02\u5206\u6790<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">dds <- DESe<span class=\"code-snippet__string\">q(dds)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u63d0\u53d6\u7ed3\u679c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">result <- as.data.frame(results(dds)) <span class=\"code-snippet__comment\"># results()\u4eceDESeq\u5206\u6790\u4e2d\u63d0\u53d6\u51fa\u7ed3\u679c\u8868<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">DGE <-subset(result,padj < <span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">05<\/span> & (log2FoldChange > <span class=\"code-snippet__number\">2<\/span> | log2FoldChange < -<span class=\"code-snippet__number\">2<\/span>)) <span class=\"code-snippet__comment\">#\u8fd9\u91cc\u6211\u4eec\u63d0\u53d6\u51fa2\u500d\u5dee\u5f02\u8868\u8fbe\u3001\u6821\u6b63p\u503c<0.05\u7684\u663e\u8457\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">DGE <- DGE[order(DGE$log2FoldChange),]<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">write.csv(DGE,<span class=\"code-snippet__string\">'DGE.csv'<\/span>,row.names = TRUE)<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<\/section>\n<p>\u6211\u4eec\u4e00\u8d77\u6765\u770b\u4e0b\u5206\u6790\u540e\u7684\u7ed3\u679c\u5427\uff01<span style=\"mso-spacerun:'yes';font-family:'Open Sans';mso-fareast-font-family:\u5b8b\u4f53;color:rgb(51,51,51);font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9307359307359307\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-27_\u5dee\u5f02\u5206\u6790\u9b54\u6cd5\u5e08\uff1aDESeq2\u4e0e\u57fa\u56e0\u7684\u9b54\u5e7b\u5192\u9669\uff01\/5_XLibVVzQX7uJeSIxgwyDDCCLDbry4w.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwx6zS7gxC4A1mawj0UyqM5JTiau35GgXyL4uKQoaiat5XCgqBXLibVVzQX7uJeSIxgwyDDCCLDbry4w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"693\" style=\"\" \/><\/p>\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.578px;text-wrap: wrap;background-color: rgb(255, 255, 255);\">\n<section>\n<section data-id=\"22363\" powered-by=\"xmyeditor.com\" data-type=\"lspecial02\" data-md5=\"7ab9d\" style=\"font-size: 16px;\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"text-align: left;\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"padding-right: 10px;padding-left: 10px;display: inline-block;vertical-align: middle;border-top: 1px dotted rgb(61, 170, 214);border-bottom: 1px dotted rgb(61, 170, 214);\">\n<section powered-by=\"xmyeditor.com\" data-md5=\"7ab9d\" style=\"color: rgb(61, 170, 214);letter-spacing: 1px;line-height: 24px;text-align: justify;\">\n<p><strong><span style=\"color: rgb(0, 122, 170);\">4\u3001\u7ed8\u5236\u706b\u5c71\u56fe<\/span><\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;padding-right: 15px;padding-left: 15px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.578px;text-wrap: wrap;outline: 0px;background-color: rgb(255, 255, 255);visibility: visible;\">\n<section>\n<p><br \/><\/p>\n<p>\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u53ef\u4ee5\u901a\u8fc7\u706b\u5c71\u56fe\u53ef\u89c6\u5316\u6211\u4eec\u7684\u5206\u6790\u7ed3\u679c\uff0c\u5177\u4f53\u64cd\u4f5c\u5982\u4e0b\uff1a<o:p><\/o:p><\/p>\n<p>\u8fd9\u91cc\u5c0f\u679c\u4f7f\u7528ggplot()\u51fd\u6570\u521b\u5efa\u7ed8\u56fe\u5bf9\u8c61\uff0c\u5e76\u8bbe\u7f6e\u6570\u636e\u6e90\u4e3aresult\u6570\u636e\u6846\u3002\u7136\u540e\u4f7f\u7528geom_point()\u51fd\u6570\u7ed8\u5236\u6563\u70b9\u56fe\uff0c\u5176\u4e2dx\u8f74\u4e3alog2FoldChange\uff0cy\u8f74\u4e3a-log10(padj)\uff0c\u5e76\u6839\u636e\u5dee\u5f02\u8868\u8fbe\u7684\u65b9\u5411\u548c\u663e\u8457\u6027\u8fdb\u884c\u989c\u8272\u5206\u7c7b\u3002\u901a\u8fc7\u8c03\u6574\u7ed8\u56fe\u7684\u5404\u79cd\u53c2\u6570\uff0c\u5982\u6807\u9898\u3001\u5750\u6807\u8f74\u6807\u7b7e\u548c\u56fe\u4f8b\uff0c\u53ef\u4ee5\u4f7f\u706b\u5c71\u56fe\u66f4\u5177\u53ef\u8bfb\u6027\u548c\u7f8e\u89c2\u6027\u54e6\u3002 <o:p><\/o:p><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">## 5.\u63d0\u53d6\u7ed3\u679c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">result <- as.data.frame(results(dds)) <span class=\"code-snippet__comment\"># results()\u4eceDESeq\u5206\u6790\u4e2d\u63d0\u53d6\u51fa\u7ed3\u679c\u8868<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">result <- na.omit(result)<\/span><\/code><code><span class=\"code-snippet_outer\">result1 <- result[c(<span class=\"code-snippet__number\">2<\/span>,<span class=\"code-snippet__number\">6<\/span>)]<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">## 6.\u706b\u5c71\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">result1$change = ifelse(result1$padj < <span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">05<\/span> & <span class=\"code-snippet__keyword\">abs<\/span>(result1$log2FoldChange) >= <span class=\"code-snippet__number\">2<\/span>, ifelse(result1$log2FoldChange> <span class=\"code-snippet__number\">2<\/span> ,<span class=\"code-snippet__string\">'Up'<\/span>,<span class=\"code-snippet__string\">'Down'<\/span>),<span class=\"code-snippet__string\">'Stable'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">library(<span class=\"code-snippet__string\">'ggplot2'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">ggplot(result1, aes(<span class=\"code-snippet__keyword\">x<\/span> = log2FoldChange, <span class=\"code-snippet__keyword\">y<\/span> = -log1<span class=\"code-snippet__number\">0<\/span>(padj),colour=change)) +<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_point(alpha=<span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">3<\/span>, size=<span class=\"code-snippet__number\">3.5<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_color_manual(<span class=\"code-snippet__keyword\">values<\/span>=c(<span class=\"code-snippet__string\">\"#546de5\"<\/span>,<span class=\"code-snippet__string\">\"#d2dae2\"<\/span>,<span class=\"code-snippet__string\">\"#ff4757\"<\/span>))+<\/span><\/code><code><span class=\"code-snippet_outer\"> labs(title=<span class=\"code-snippet__string\">'Volcano plot'<\/span>,<span class=\"code-snippet__keyword\">x<\/span>=<span class=\"code-snippet__string\">\"log2(fold change)\"<\/span>,<span class=\"code-snippet__keyword\">y<\/span>=<span class=\"code-snippet__string\">\"-log10 (padj)\"<\/span>)+ <span class=\"code-snippet__comment\"># \u5750\u6807\u8f74# \u5750\u6807\u8f74\u548c\u56fe\u6807\u9898title=\"Volcano plot\",<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> theme_bw()+ <span class=\"code-snippet__comment\">#\u53bb\u9664\u80cc\u666f\u8272<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\">#xlim(-20, 20)+ #\u8bbe\u7f6e\u5750\u6807\u8f74\u8303\u56f4<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\">#ylim(0,40)+<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\"># \u8f85\u52a9\u7ebf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> geom_vline(xintercept=c(-<span class=\"code-snippet__number\">2<\/span>,<span class=\"code-snippet__number\">2<\/span>),lty=<span class=\"code-snippet__number\">3<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">8<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_hline(yintercept = -log1<span class=\"code-snippet__number\">0<\/span>(<span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">05<\/span>),lty=<span class=\"code-snippet__number\">3<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">8<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> theme(panel.grid = element_blank())+ <span class=\"code-snippet__comment\">#\u53bb\u9664\u7f51\u683c\u7ebf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> theme(plot.title = element_text(hjust = <span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">5<\/span>,size=<span class=\"code-snippet__number\">24<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.position=<span class=\"code-snippet__string\">\"bottom\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.title = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.text=element_text(size=<span class=\"code-snippet__number\">18<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.key.size = unit(<span class=\"code-snippet__number\">1<\/span>, <span class=\"code-snippet__string\">'cm'<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.background = element_rect(fill=<span class=\"code-snippet__string\">\"gray90\"<\/span>, linetype=<span class=\"code-snippet__string\">\"solid\"<\/span>,colour =<span class=\"code-snippet__string\">\"gray\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.title.x =element_text(size=<span class=\"code-snippet__number\">18<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.title.y=element_text(size=<span class=\"code-snippet__number\">18<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.text=element_text(size=<span class=\"code-snippet__number\">14<\/span>,face = <span class=\"code-snippet__string\">\"bold\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> )<\/span><\/code><code><span class=\"code-snippet_outer\">ggsave(<span class=\"code-snippet__string\">\"De_ana.png\"<\/span>,width=<span class=\"code-snippet__number\">8<\/span>,height=<span class=\"code-snippet__number\">6<\/span>)<span class=\"code-snippet__comment\"># \u4fdd\u5b58\u4e3apng\u683c\u5f0f<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p>\u6211\u4eec\u4e00\u8d77\u6765\u770b\u4e0b\u6700\u540e\u7684\u7ed3\u679c\u56fe\u5427\uff01<o:p><\/o:p><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.7503607503607503\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_czqOv8XDtgamzict1qib2qe7qxqh2w.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwx6zS7gxC4A1mawj0UyqM5TREZGJoC4GoibR8ib9Q3HgvEw2pniczqOv8XDtgamzict1qib2qe7qxqh2w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"693\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p><br \/><\/p>\n<p>\u600e\u4e48\u6837\uff0c\u4f60\u5b66\u4f1a\u600e\u4e48\u4f7f\u7528DESeq2\u5305\u6765\u8fdb\u884c\u5dee\u5f02\u5206\u6790\u4e86\u561b\uff1f\u662f\u4e0d\u662f\u5f88\u7b80\u5355\uff01\u66f4\u591a\u5b66\u4e60\u5e72\u6d3b\u8981\u7ee7\u7eed\u5173\u6ce8\u5c0f\u679c\u54e6\uff01<o:p><\/o:p><\/p>\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section>\n<p><span style=\"font-size: 14px;color: rgb(0, 122, 170);\">\u8fd9\u91cc\u63a8\u8350\u4e00\u4e0b\u5c0f\u679c\u65b0\u5f00\u53d1\u7684\u96f6\u4ee3\u7801\u4e91\u751f\u4fe1\u5206\u6790\u5de5\u5177\u5e73\u53f0\u5305\u542b\u8d85\u591a\u96f6\u4ee3\u7801\u5c0f\u5de5\u5177\uff0c\u4e0a\u4f20\u6570\u636e\u4e00\u952e\u51fa\u56fe\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u4e86\u89e3~<\/span><span style=\"text-align: center;letter-spacing: 0.578px;font-size: var(--articleFontsize);\"><\/span><\/p>\n<p><span style=\"font-size: 14px;color: rgb(0, 122, 170);\">\u7f51\u5740\uff1ahttp:\/\/www.biocloudservice.com\/home.html<\/span><\/p>\n<p><br \/><\/p>\n<section data-id=\"75061\" data-type=\"lspecial02\" data-tools=\"\u901f\u6392\u5c0f\u8682\u8681\u7f16\u8f91\u5668\" powered-by=\"xmyeditor.com\" data-md5=\"83a15\">\n<p><span style=\"font-size: 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