<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 60px}.rich_media_area_extra{padding:0 60px 21px 60px}}@media (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner,body{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media{padding:20px;overflow:hidden;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}@media screen and (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media_content{overflow:hidden;color:#333;font-size:17px;word-wrap:break-word;-webkit-hyphens:auto;-ms-hyphens:auto;hyphens:auto;text-align:justify;position:relative;z-index:0}.rich_media_content *{max-width:100%!important;box-sizing:border-box!important;-webkit-box-sizing:border-box!important;word-wrap:break-word!important}.rich_media_content p{clear:both;min-height:1em}.rich_media_content em{font-style:italic}.rich_media_content fieldset{min-width:0}.rich_media_content .list-paddingleft-1,.rich_media_content .list-paddingleft-2,.rich_media_content .list-paddingleft-3{padding-left:2.2em}.rich_media_content .list-paddingleft-1 .list-paddingleft-2,.rich_media_content .list-paddingleft-2 .list-paddingleft-2,.rich_media_content .list-paddingleft-3 .list-paddingleft-2{padding-left:30px}.rich_media_content .list-paddingleft-1{padding-left:1.2em}.rich_media_content .list-paddingleft-3{padding-left:3.2em}.rich_media_content .code-snippet,.rich_media_content .code-snippet__fix{max-width:1000%!important}.rich_media_content .code-snippet *,.rich_media_content .code-snippet__fix *{max-width:1000%!important}.rich_media_title{font-size:22px;line-height:1.4;margin-bottom:13px;padding-bottom:13px;border-bottom:1px solid #e7e7eb;}@supports(-webkit-overflow-scrolling:touch){.rich_media_title{font-weight:700}}.rich_media_meta{display:inline-block;vertical-align:middle;padding:0 0 10px 0;font-size:15px;-webkit-tap-highlight-color:rgba(0,0,0,0)}.rich_media_meta.icon_appmsg_tag{margin-right:0px}.rich_media_meta.meta_tag_text{margin-right:0}.rich_media_meta_list em{font-style:normal}.rich_media_meta_text{color:#a5a5a5;}p{margin:0;}.msgBox{margin-top:20px;padding-top:20px;padding-left:50px;overflow:hidden;border-top:2px dashed #09a2ff;}.msg{padding-top:7px;clear:both;}.msgBody{float:right;width:100%;margin-left:55px;padding-bottom:15px;border-bottom:1px dashed #e0e0e0;}.userHeadImg{float:left;margin-left:-50px;}.userHeadImg img{width:40px;height:40px;margin-right:10px;border-radius:3px;}.userName{color:#888888;line-height:24px;font-size:14px;margin:5px 0 5px 0;height:24px;}.replyBody,.autherBody{color:#565656;font-size:15px;}.replyIcon{border-left:4px solid #33ab01;margin-right:5px;}.ad{text-decoration:none;color:#d6d4d4;font-size:12px;}.msgBodyReply{padding-top:5px;}.userName span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation Mono\",Menlo,Courier,monospace}.code-snippet__comment,.code-snippet__quote{color:#afafaf;font-style:italic}.code-snippet__keyword,.code-snippet__selector-tag,.code-snippet__subst{color:#ca7d37}.code-snippet__number,.code-snippet__literal,.code-snippet__variable,.code-snippet__template-variable,.code-snippet__tag .code-snippet__attr{color:#0e9ce5}.code-snippet__string,.code-snippet__doctag{color:#d14}.code-snippet__title,.code-snippet__section,.code-snippet__selector-id{color:#d14}.code-snippet__subst{font-weight:normal}.code-snippet__type,.code-snippet__class .code-snippet__title{color:#0e9ce5}.code-snippet__tag,.code-snippet__name,.code-snippet__attribute{color:#0e9ce5;font-weight:normal}.code-snippet__regexp,.code-snippet__link{color:#ca7d37}.code-snippet__symbol,.code-snippet__bullet{color:#d14}.code-snippet__built_in,.code-snippet__builtin-name{color:#ca7d37}.code-snippet__meta{color:#afafaf}.code-snippet__deletion{background:#fdd}.code-snippet__addition{background:#dfd}.code-snippet__emphasis{font-style:italic}.code-snippet__strong{font-weight:bold}.account_avatar{width:40px;height:40px;padding:0;}.account_info{display:-webkit-box;display:-webkit-flex;display:flex;-webkit-box-align:center;-webkit-align-items:center;padding:20px 0;align-items:center}.flex_bd{padding-left:14px;}.account_nickname{display:inline-block;vertical-align:middle;line-height:1.2;color:#576b95;font-size:14px}.account_desc{overflow:hidden;text-overflow:ellipsis;display:-webkit-box;-webkit-box-orient:vertical;-webkit-line-clamp:1;color:rgba(0,0,0,0.3);font-size:14px;line-height:1.2;padding-top:.4em}.msg_source_url{text-align:left;word-break:break-all;margin-top:20px;}.msg_source_url a{padding-right:10px;}.msg_source_url .url_text{color:#a8a8a8;}.video-desc{font-size:14px;margin-top:15px;color:#6c6c6c;}.msg_source_url{text-align:left;}.original_primary_card_tips{color:rgba(0,0,0,0.3);line-height:1.4;font-size:15px;}.weui-flex__item{margin-bottom:20px;padding:20px 16px;margin-top:16px;line-height:1.4;align-items:center;background-color:#f7f7f7;border-radius:8px;position:relative;}.original_primary_desc{color:rgba(0,0,0,0.5);font-size:14px;padding-top:4px;width:auto;overflow:hidden;text-overflow:ellipsis;}.msgBodyReplyList{border-top:1px solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar 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id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-09-04 19:02:21<\/em><\/div>\n<p><content><\/p>\n<div class=\"reprint\">\n<p>\u8f6c\u81ea\u516c\u4f17\u53f7\uff1a\u751f\u4fe1\u82b1<br \/><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzkzODUxNjcwMA==&mid=2247484326&idx=1&sn=bac02ef8af0c2cedfa69665220c2bed6\" target=\"_blank\" title=\"\u9605\u8bfb\u539f\u6587\" rel=\"noopener\">http:\/\/mp.weixin.qq.com\/s?__biz=MzkzODUxNjcwMA==&mid=2247484326&idx=1&sn=bac02ef8af0c2cedfa69665220c2bed6<\/a><\/p>\n<\/div>\n<section style=\"font-size: 14px;margin-bottom: 24px;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(65, 128, 216);\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;padding-left: 12px;\">\n<section style=\"text-align: justify;color: rgb(255, 255, 255);\" powered-by=\"xiumi.us\">\n<p><strong>\u70b9\u51fb\u84dd\u5b57 \u5173\u6ce8\u6211\u4eec<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;margin-right: -17px;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;margin-right: -17px;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(201, 229, 70);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u4eca\u5929\u5c0f\u82b1\u5c31\u4e3a\u5927\u5bb6\u4ecb\u7ecd\u4e00\u4e0b\u5b8f\u57fa\u56e0\u7ec4\u5206\u6790\u7684\u7b2c\u4e00\u6b65\u2014\u8d28\u63a7\u4e2d\u8d28\u91cf\u8bc4\u4f30\u8f6f\u4ef6FastQC\u7684\u4f7f\u7528\u548c\u7ed3\u679c\u89e3\u8bfb\u3002<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">FastQC\u7528\u4e8e\u5feb\u901f\u8bc4\u4f30\u6d4b\u5e8f\u6570\u636e\u7684\u8d28\u91cf\u3002\u5b83\u80fd\u591f\u751f\u6210\u8d28\u91cf\u62a5\u544a\uff0c\u663e\u793a\u5404\u4e2a\u78b1\u57fa\u7684\u8d28\u91cf\u5206\u6570\u548c\u5e8f\u5217\u8d28\u91cf\u5206\u5e03\uff0c\u5e2e\u52a9\u68c0\u6d4b\u4f4e\u8d28\u91cf\u7684\u78b1\u57fa\u548c\u6f5c\u5728\u7684\u6d4b\u5e8f\u95ee\u9898\u3002\u63a5\u4e0b\u6765\u5c0f\u82b1\u5c31\u6765\u6559\u5927\u5bb6\u5982\u4f55\u5b89\u88c5\u548c\u4f7f\u7528FastQC\u3002<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;border-width: 1px;border-radius: 8px;border-style: dotted;border-color: rgb(0, 0, 0);overflow: hidden;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin: 15px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);padding-right: 15px;padding-left: 15px;\">\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">\u9996\u5148\u6211\u4eec\u9700\u8981\u5b89\u88c5\uff0c\u53ef\u4ee5\u7528conda\u5b89\u88c5\uff1a<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">conda install FastQC<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">\u5f53\u7136conda\u6709\u65f6\u5019\u5b89\u88c5\u8f83\u6162\u4e14\u53ef\u80fd\u5b89\u88c5\u5931\u8d25\uff0c\u5927\u5bb6\u53ef\u4ee5\u5230\u5b98\u7f51\u4e0b\u8f7d\u4e4b\u540e\u89e3\u538b\uff1ahttp:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/fastqc_v0.11.3.zip\uff0c\u6216\u8005\u5728linux\u4e0a\u7528\u547d\u4ee4\uff1a<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">wget http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/fastqc_v0.11.3.zip<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">unzip fastqc_v0.11.3.zip<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">\u8fdb\u5165\u89e3\u538b\u76ee\u5f55\uff0c\u8bbe\u7f6e\u6743\u9650\uff0c\u914d\u7f6e\u73af\u5883\u53d8\u91cf\uff1a<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">cd FastQC\/<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">chmod 755 fastqc<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">export PATH=\/home\/user\/FastQC\/:$PATH<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">\u6d4b\u8bd5\u4e00\u4e0b\uff1a<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">fastqc \u2013help<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">\u5982\u679c\u80fd\u770b\u5230\u5e2e\u52a9\u6587\u4ef6\u8bf4\u660e\u5c31\u5b89\u88c5\u6210\u529f\u4e86\u3002\u4e4b\u540e\u521b\u5efa\u4e00\u4e2a\u7ed3\u679c\u6587\u4ef6\u5939qc\uff0c\u5f00\u59cb\u8fd0\u884c\uff1a<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">mkdir qc<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">fastqc –outdir qc #\u8f93\u51fa\u6587\u4ef6\u5939<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">–threads 8#\u7ebf\u7a0b\u6570<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">A1.fq.gz#\u5e8f\u5217\u6587\u4ef6<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">A2.fq.gz<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">\u6bcf\u4e2a\u5e8f\u5217\u751f\u6210\u4e24\u4e2a\u7ed3\u679c\u6587\u4ef6\uff0c1\u4e2a\u7f51\u9875\u62a5\u544a\u6587\u4ef6\u548c1\u4e2a\u8d28\u63a7\u540e\u7684zip\u6587\u4ef6\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u5c0f\u82b1\u4e3b\u8981\u5bf9\u7f51\u9875\u7248\u4e3a\u5927\u5bb6\u89e3\u8bfb\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5611111111111111\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-04_\u300c\u8f6c\u300d\u5b8f\u57fa\u56e0\u7ec4\u8d28\u63a7\u8fc7\u7a0b\u4e2dFastQC\u4f7f\u7528\u53ca\u7ed3\u679c\u89e3\u8bfb\/1_SaAMv2MILHc3QJ8QD3GlgIS9sBibOQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZZ1lrHwicfSF2w0G87tDFBOdMsXo9BVkBYEibJQJj32ocY7Y7SaAMv2MILHc3QJ8QD3GlgIS9sBibOQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;border-width: 1px;border-radius: 8px;border-style: dotted;border-color: rgb(0, 0, 0);overflow: hidden;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin: 15px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);padding-right: 15px;padding-left: 15px;\">\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">Filename\u662f\u5e8f\u5217\u6587\u4ef6\u540d\u79f0<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">Encoding\u6307\u6d4b\u5e8f\u5e73\u53f0\u7684\u7248\u672c\u548c\u76f8\u5e94\u7684\u7f16\u7801\u7248\u672c\u53f7\uff0c\u53ef\u770b\u51fa\u662fPhred 33 \u6216\u662fPhred 64 \u8d28\u91cf\u5206\u6570\u7684\u7f16\u7801\u65b9\u5f0f\u3002<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">Total Sequences\u8f93\u5165reads\u603b\u6570\u91cf\u3002<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">Sequence length \u662f\u6d4b\u5e8f\u7684\u957f\u5ea6\u3002<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">%GC \u662f\u6211\u4eec\u9700\u8981\u91cd\u70b9\u5173\u6ce8\u7684\u4e00\u4e2a\u6307\u6807\uff0c\u8fd9\u4e2a\u503c\u8868\u793a\u7684\u662f\u5168\u90e8\u5e8f\u5217\u4e2d\u7684GC\u542b\u91cf\uff0c\u8fd9\u4e2a\u6570\u503c\u4e00\u822c\u662f\u7269\u79cd\u7279\u5f02\u7684\uff0c\u56e0\u6b64\u5982\u679c\u5b9e\u9a8c\u6570\u636e\u4e0d\u540c\u5206\u7ec4\u4e4b\u95f4\u7684\u5fae\u751f\u7269\u7ec4\u6210\u548c\u529f\u80fd\u6709\u5dee\u5f02\uff0c%GC\u8fd9\u4e2a\u503c\u5927\u6982\u7387\u4e5f\u662f\u6709\u5dee\u5f02\u7684\u3002<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">\u5de6\u8fb9summary\u91cc\u9762\u7eff\u8272\u7684\u52fe\u662f\u5408\u683c\uff0c\u8b66\u544a\u662f\u9ec4\u8272\u53f9\u53f7\uff0c\u4e0d\u5408\u683c\u662f\u7ea2\u53c9\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u63a5\u4e0b\u6765\u5c0f\u82b1\u5c31\u4ece\u4e0a\u5230\u4e0b\u4f9d\u6b21\u4e3a\u5927\u5bb6\u5c55\u793a\u548c\u5206\u6790\u7ed3\u679c\u56fe\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7550403225806451\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_073FCvfn4qULVKYxSqoX2rwszKe8TQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZZ1lrHwicfSF2w0G87tDFBO4jTHfBbRbNPxoCsgh4BJKl0b073FCvfn4qULVKYxSqoX2rwszKe8TQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"992\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u4e2a\u56fe\u6a2a\u8f74\u4e3aread\u957f\u5ea6\uff0c\u7eb5\u8f74\u4e3a\u8d28\u91cf\u5f97\u5206\uff0c\u67f1\u72b6\u8868\u793a\u8be5\u78b1\u57fa\u4f4d\u7f6e\u6240\u6709\u5e8f\u5217\u7684\u6d4b\u5e8f\u8d28\u91cf\u7684\u7edf\u8ba1\uff0c\u6211\u4eec\u8fd9\u4e2areads\u6bcf\u4e2a\u78b1\u57fa\u8d28\u91cf\u90fd\u662f\u5f88\u597d\u7684\u3002<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7421638018200202\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-04_\u300c\u8f6c\u300d\u5b8f\u57fa\u56e0\u7ec4\u8d28\u63a7\u8fc7\u7a0b\u4e2dFastQC\u4f7f\u7528\u53ca\u7ed3\u679c\u89e3\u8bfb\/3_djkv7nHFBk3Oia2QEvlQwAk2fibBvw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZZ1lrHwicfSF2w0G87tDFBOfEzBMDOiaNMkL3rAH2eXewJJbOicdjkv7nHFBk3Oia2QEvlQwAk2fibBvw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"989\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u4e2a\u56fe\u662f\u6bcf\u6761reads\u7684quality\u7684\u5747\u503c\u7684\u5206\u5e03\uff0c\u6a2a\u8f74\u8868\u793a\u8d28\u91cf\u5206\u6570\uff0c\u7eb5\u8f74\u8868\u793a\u6bcf\u4e2a\u503c\u5bf9\u5e94\u7684reads\u6570\u76ee\uff0c\u5f53\u6d4b\u5e8f\u7ed3\u679c\u4e3b\u8981\u96c6\u4e2d\u5728\u9ad8\u5206\u4e2d\uff0c\u8bc1\u660e\u6d4b\u5e8f\u8d28\u91cf\u826f\u597d\u3002<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7463465553235908\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_EVa30ibRkjYchibL4MS85mbdnZ9wcg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZZ1lrHwicfSF2w0G87tDFBOf2HRkXdkPkrvgxibJRZVyqE8oPWEVa30ibRkjYchibL4MS85mbdnZ9wcg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"958\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u4e2a\u56fe\u662f\u5bf9\u6240\u6709reads\u7684\u6bcf\u4e00\u4e2a\u4f4d\u7f6e\u7684ATCG\u56db\u79cd\u78b1\u57fa\u7684\u5206\u5e03\u56fe\uff0c\u7528\u4e8e\u68c0\u67e5\u662f\u5426\u6709AT,GC\u5206\u79bb\u73b0\u8c61\u3002\u6a2a\u8f74\u4e3a\u78b1\u57fa\u957f\u5ea6\u5206\u5e03\uff0c\u7eb5\u8f74\u8868\u793a\u8be5\u4f4d\u7f6e\u6240\u542b\u5bf9\u5e94\u78b1\u57fa\u7684\u767e\u5206\u6bd4\uff0c\u56fe\u4e2d4\u6761\u7ebf\u5206\u522b\u4ee3\u8868A\uff0cC\uff0cT\uff0cG\u5728\u6bcf\u4e2a\u4f4d\u7f6e\u4e0a\u7684\u5e73\u5747\u542b\u91cf\u3002<\/p>\n<p style=\"text-indent: 2em;text-wrap: wrap;\"><br \/><\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u63a5\u7740\u6211\u4eec\u5c31\u53ef\u4ee5\u4f7f\u7528fastp\u8fdb\u884c\u8d28\u91cf\u63a7\u5236\u3001\u9002\u914d\u4f53\u4fee\u526a\u3001NGS\u5f15\u7269\u4fee\u526a\u7b49\u8d28\u63a7\u8fc7\u7a0b\u5566\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7533960292580982\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-04_\u300c\u8f6c\u300d\u5b8f\u57fa\u56e0\u7ec4\u8d28\u63a7\u8fc7\u7a0b\u4e2dFastQC\u4f7f\u7528\u53ca\u7ed3\u679c\u89e3\u8bfb\/5_4bzNtB5LcIDBdNZdibSZjibP4JdnTw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZZ1lrHwicfSF2w0G87tDFBO4CXicM8JIg9vlC7heZsfVial4VZp4bzNtB5LcIDBdNZdibSZjibP4JdnTw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"957\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u4e2a\u56fe\u7edf\u8ba1reads\u7684\u5e73\u5747GC\u542b\u91cf\u7684\u5206\u5e03\uff0c\u7ea2\u7ebf\u662f\u6211\u4eec\u7684\u6570\u636e\u7684\u60c5\u51b5\uff0c\u84dd\u7ebf\u662f\u7406\u8bba\u5206\u5e03\uff08\u6b63\u6001\u5206\u5e03\uff09\uff0c\u6ce8\u610f\u8fd9\u513f\u5c0f\u82b1\u4e5f\u662f\u4e00\u4e2a\u00d7\uff0c\u4e0d\u8fc7\u5b8f\u57fa\u56e0\u7ec4\u6570\u636e\u7684GC\u542b\u91cf\u4e0d\u5408\u683c\u662f\u5f88\u6b63\u5e38\u7684\u3002<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.749738219895288\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_4zevvQUjUut8HFhvWyZp1jpFbhmkNQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZZ1lrHwicfSF2w0G87tDFBOa9hwBh9pyzWrDQDwS9d2bNyz4zevvQUjUut8HFhvWyZp1jpFbhmkNQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"955\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u4e2a\u56fe\u662f\u663e\u793areads\u6bcf\u4e2a\u4f4d\u7f6e\u51fa\u73b0N\u7684\u6bd4\u7387\u3002\u5c0f\u82b1\u4e3a\u5927\u5bb6\u89e3\u91ca\u4e00\u4e0bN\u662f\u4ec0\u4e48\uff1a\u5f53\u51fa\u73b0\u6d4b\u5e8f\u4eea\u4e0d\u80fd\u5206\u8fa8\u7684\u78b1\u57fa\u65f6\u4f1a\u4ea7\u751fN\uff0c\u56e0\u6b64\u8fc7\u591a\u7684N\u5c31\u4ee3\u8868\u6211\u4eec\u7684\u6d4b\u5e8f\u6570\u636e\u8d28\u91cf\u5e76\u4e0d\u9ad8\u3002<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7458333333333333\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-04_\u300c\u8f6c\u300d\u5b8f\u57fa\u56e0\u7ec4\u8d28\u63a7\u8fc7\u7a0b\u4e2dFastQC\u4f7f\u7528\u53ca\u7ed3\u679c\u89e3\u8bfb\/7_3B3LnicyUHgLa1KRPmdhaCZiaNuE6g.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZZ1lrHwicfSF2w0G87tDFBOjvlaMbNvCfXjajeFiaAmNWI8KGo3B3LnicyUHgLa1KRPmdhaCZiaNuE6g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"960\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u4e2a\u56fe\u662freads\u957f\u5ea6\u7684\u5206\u5e03\uff0c\u8d28\u91cf\u597d\u7684\u6570\u636e\u5f80\u5f80reads\u7684\u957f\u5ea6\u90fd\u96c6\u4e2d\u5206\u5e03\u5728\u4e00\u4e2a\u5730\u65b9\u3002<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7502601456815817\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_Qicea8BQnRYK7d7TAhm0BTLvzwnb5w.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZZ1lrHwicfSF2w0G87tDFBOKqQdxic0SI0a0AJLqeXe9IoATLQicea8BQnRYK7d7TAhm0BTLvzwnb5w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"961\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u4e2a\u56fe\u662f\u7edf\u8ba1\u5e8f\u5217\u5b8c\u5168\u4e00\u81f4\u7684reads\u7684\u9891\u7387\uff0c\u6a2a\u5750\u6807\u662fduplication\u7684\u6b21\u6570\uff0c\u7eb5\u5750\u6807\u662fduplicated reads\u7684\u6570\u76ee\uff0c\u4ee5unique reads\u7684\u603b\u6570\u4f5c\u4e3a100%\u3002<\/p>\n<p style=\"text-indent: 2em;text-wrap: wrap;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.2411467116357504\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-04_\u300c\u8f6c\u300d\u5b8f\u57fa\u56e0\u7ec4\u8d28\u63a7\u8fc7\u7a0b\u4e2dFastQC\u4f7f\u7528\u53ca\u7ed3\u679c\u89e3\u8bfb\/9_PosOyCJMA1qy8klZnR7QLx7vTr0PBw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZZ1lrHwicfSF2w0G87tDFBOiaEwuyK4KvOSWDks6fVnbFKzwPosOyCJMA1qy8klZnR7QLx7vTr0PBw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"593\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u4e2a\u7ed3\u679c\u8bf4\u660e\u6211\u4eec\u6570\u636e\u4e2d\u6ca1\u6709over-represented\u7684\u5e8f\u5217\uff0c\u5373\u6ca1\u6709\u67d0\u4e2a\u5927\u91cf\u51fa\u73b0\u7684\u5e8f\u5217\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7549738219895288\" data-s=\"300,640\" 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style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650761303&idx=2&sn=6f73471d1e82c56c0b2160d0fcd32d53&scene=21#wechat_redirect\" textvalue=\"\" linktype=\"image\" imgurl=\"https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/0?wx_fmt=jpeg\" imgdata=\"[object Object]\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.35083798882681566\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_pBN4r4MaBCk9p9v16yZwvMvS5nM7sQ-5.jpg\" 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href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5b8f\u57fa\u56e0\u7ec4\u8d28\u63a7\u8fc7\u7a0b\u4e2dFastQC\u4f7f\u7528\u53ca\u7ed3\u679c\u89e3\u8bfb \u5c0f\u82b1 \u751f\u4fe1\u679c 202 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{"id":54831,"date":"2024-06-16T14:30:03","date_gmt":"2024-06-16T06:30:03","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=54831"},"modified":"2024-06-16T14:30:03","modified_gmt":"2024-06-16T06:30:03","slug":"%e5%ae%8f%e5%9f%ba%e5%9b%a0%e7%bb%84%e8%b4%a8%e6%8e%a7%e8%bf%87%e7%a8%8b%e4%b8%adfastqc%e4%bd%bf%e7%94%a8%e5%8f%8a%e7%bb%93%e6%9e%9c%e8%a7%a3%e8%af%bb","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=54831","title":{"rendered":"\u5b8f\u57fa\u56e0\u7ec4\u8d28\u63a7\u8fc7\u7a0b\u4e2dFastQC\u4f7f\u7528\u53ca\u7ed3\u679c\u89e3\u8bfb"},"content":{"rendered":"

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0;align-items:center}.flex_bd{padding-left:14px;}.account_nickname{display:inline-block;vertical-align:middle;line-height:1.2;color:#576b95;font-size:14px}.account_desc{overflow:hidden;text-overflow:ellipsis;display:-webkit-box;-webkit-box-orient:vertical;-webkit-line-clamp:1;color:rgba(0,0,0,0.3);font-size:14px;line-height:1.2;padding-top:.4em}.msg_source_url{text-align:left;word-break:break-all;margin-top:20px;}.msg_source_url a{padding-right:10px;}.msg_source_url .url_text{color:#a8a8a8;}.video-desc{font-size:14px;margin-top:15px;color:#6c6c6c;}.msg_source_url{text-align:left;}.original_primary_card_tips{color:rgba(0,0,0,0.3);line-height:1.4;font-size:15px;}.weui-flex__item{margin-bottom:20px;padding:20px 16px;margin-top:16px;line-height:1.4;align-items:center;background-color:#f7f7f7;border-radius:8px;position:relative;}.original_primary_desc{color:rgba(0,0,0,0.5);font-size:14px;padding-top:4px;width:auto;overflow:hidden;text-overflow:ellipsis;}.msgBodyReplyList{border-top:1px solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"\u5b8f\u57fa\u56e0\u7ec4\u8d28\u63a7\u8fc7\u7a0b\u4e2dFastQC\u4f7f\u7528\u53ca\u7ed3\u679c\u89e3\u8bfb\",\"time\":\"2023-09-04 19:02:21\",\"timeStamp\":\"1693825341\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491909&idx=4&sn=4ae94a2d06e86d0fb57130b3451f0f54&chksm=cf7f7180f808f8960ccddfec7ba0bf26522a2b009929daadc718177da6255548166279ce27af#rd\" target=\"_blank\" rel=\"noopener\">\u5b8f\u57fa\u56e0\u7ec4\u8d28\u63a7\u8fc7\u7a0b\u4e2dFastQC\u4f7f\u7528\u53ca\u7ed3\u679c\u89e3\u8bfb<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u82b1 <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-09-04 19:02:21<\/em><\/div>\n<p><content><\/p>\n<div class=\"reprint\">\n<p>\u8f6c\u81ea\u516c\u4f17\u53f7\uff1a\u751f\u4fe1\u82b1<br \/><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzkzODUxNjcwMA==&mid=2247484326&idx=1&sn=bac02ef8af0c2cedfa69665220c2bed6\" target=\"_blank\" title=\"\u9605\u8bfb\u539f\u6587\" rel=\"noopener\">http:\/\/mp.weixin.qq.com\/s?__biz=MzkzODUxNjcwMA==&mid=2247484326&idx=1&sn=bac02ef8af0c2cedfa69665220c2bed6<\/a><\/p>\n<\/div>\n<section style=\"font-size: 14px;margin-bottom: 24px;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(65, 128, 216);\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;padding-left: 12px;\">\n<section style=\"text-align: justify;color: rgb(255, 255, 255);\" powered-by=\"xiumi.us\">\n<p><strong>\u70b9\u51fb\u84dd\u5b57 \u5173\u6ce8\u6211\u4eec<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;margin-right: -17px;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;margin-right: -17px;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(201, 229, 70);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u4eca\u5929\u5c0f\u82b1\u5c31\u4e3a\u5927\u5bb6\u4ecb\u7ecd\u4e00\u4e0b\u5b8f\u57fa\u56e0\u7ec4\u5206\u6790\u7684\u7b2c\u4e00\u6b65\u2014\u8d28\u63a7\u4e2d\u8d28\u91cf\u8bc4\u4f30\u8f6f\u4ef6FastQC\u7684\u4f7f\u7528\u548c\u7ed3\u679c\u89e3\u8bfb\u3002<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">FastQC\u7528\u4e8e\u5feb\u901f\u8bc4\u4f30\u6d4b\u5e8f\u6570\u636e\u7684\u8d28\u91cf\u3002\u5b83\u80fd\u591f\u751f\u6210\u8d28\u91cf\u62a5\u544a\uff0c\u663e\u793a\u5404\u4e2a\u78b1\u57fa\u7684\u8d28\u91cf\u5206\u6570\u548c\u5e8f\u5217\u8d28\u91cf\u5206\u5e03\uff0c\u5e2e\u52a9\u68c0\u6d4b\u4f4e\u8d28\u91cf\u7684\u78b1\u57fa\u548c\u6f5c\u5728\u7684\u6d4b\u5e8f\u95ee\u9898\u3002\u63a5\u4e0b\u6765\u5c0f\u82b1\u5c31\u6765\u6559\u5927\u5bb6\u5982\u4f55\u5b89\u88c5\u548c\u4f7f\u7528FastQC\u3002<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;border-width: 1px;border-radius: 8px;border-style: dotted;border-color: rgb(0, 0, 0);overflow: hidden;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin: 15px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);padding-right: 15px;padding-left: 15px;\">\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">\u9996\u5148\u6211\u4eec\u9700\u8981\u5b89\u88c5\uff0c\u53ef\u4ee5\u7528conda\u5b89\u88c5\uff1a<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">conda install FastQC<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">\u5f53\u7136conda\u6709\u65f6\u5019\u5b89\u88c5\u8f83\u6162\u4e14\u53ef\u80fd\u5b89\u88c5\u5931\u8d25\uff0c\u5927\u5bb6\u53ef\u4ee5\u5230\u5b98\u7f51\u4e0b\u8f7d\u4e4b\u540e\u89e3\u538b\uff1ahttp:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/fastqc_v0.11.3.zip\uff0c\u6216\u8005\u5728linux\u4e0a\u7528\u547d\u4ee4\uff1a<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">wget http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/fastqc_v0.11.3.zip<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">unzip fastqc_v0.11.3.zip<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">\u8fdb\u5165\u89e3\u538b\u76ee\u5f55\uff0c\u8bbe\u7f6e\u6743\u9650\uff0c\u914d\u7f6e\u73af\u5883\u53d8\u91cf\uff1a<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">cd FastQC\/<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">chmod 755 fastqc<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">export PATH=\/home\/user\/FastQC\/:$PATH<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">\u6d4b\u8bd5\u4e00\u4e0b\uff1a<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">fastqc \u2013help<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">\u5982\u679c\u80fd\u770b\u5230\u5e2e\u52a9\u6587\u4ef6\u8bf4\u660e\u5c31\u5b89\u88c5\u6210\u529f\u4e86\u3002\u4e4b\u540e\u521b\u5efa\u4e00\u4e2a\u7ed3\u679c\u6587\u4ef6\u5939qc\uff0c\u5f00\u59cb\u8fd0\u884c\uff1a<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">mkdir qc<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">fastqc –outdir qc #\u8f93\u51fa\u6587\u4ef6\u5939<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">–threads 8#\u7ebf\u7a0b\u6570<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">A1.fq.gz#\u5e8f\u5217\u6587\u4ef6<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">A2.fq.gz<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">\u6bcf\u4e2a\u5e8f\u5217\u751f\u6210\u4e24\u4e2a\u7ed3\u679c\u6587\u4ef6\uff0c1\u4e2a\u7f51\u9875\u62a5\u544a\u6587\u4ef6\u548c1\u4e2a\u8d28\u63a7\u540e\u7684zip\u6587\u4ef6\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u5c0f\u82b1\u4e3b\u8981\u5bf9\u7f51\u9875\u7248\u4e3a\u5927\u5bb6\u89e3\u8bfb\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5611111111111111\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-04_\u300c\u8f6c\u300d\u5b8f\u57fa\u56e0\u7ec4\u8d28\u63a7\u8fc7\u7a0b\u4e2dFastQC\u4f7f\u7528\u53ca\u7ed3\u679c\u89e3\u8bfb\/1_SaAMv2MILHc3QJ8QD3GlgIS9sBibOQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZZ1lrHwicfSF2w0G87tDFBOdMsXo9BVkBYEibJQJj32ocY7Y7SaAMv2MILHc3QJ8QD3GlgIS9sBibOQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;border-width: 1px;border-radius: 8px;border-style: dotted;border-color: rgb(0, 0, 0);overflow: hidden;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin: 15px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);padding-right: 15px;padding-left: 15px;\">\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">Filename\u662f\u5e8f\u5217\u6587\u4ef6\u540d\u79f0<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">Encoding\u6307\u6d4b\u5e8f\u5e73\u53f0\u7684\u7248\u672c\u548c\u76f8\u5e94\u7684\u7f16\u7801\u7248\u672c\u53f7\uff0c\u53ef\u770b\u51fa\u662fPhred 33 \u6216\u662fPhred 64 \u8d28\u91cf\u5206\u6570\u7684\u7f16\u7801\u65b9\u5f0f\u3002<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">Total Sequences\u8f93\u5165reads\u603b\u6570\u91cf\u3002<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">Sequence length \u662f\u6d4b\u5e8f\u7684\u957f\u5ea6\u3002<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">%GC \u662f\u6211\u4eec\u9700\u8981\u91cd\u70b9\u5173\u6ce8\u7684\u4e00\u4e2a\u6307\u6807\uff0c\u8fd9\u4e2a\u503c\u8868\u793a\u7684\u662f\u5168\u90e8\u5e8f\u5217\u4e2d\u7684GC\u542b\u91cf\uff0c\u8fd9\u4e2a\u6570\u503c\u4e00\u822c\u662f\u7269\u79cd\u7279\u5f02\u7684\uff0c\u56e0\u6b64\u5982\u679c\u5b9e\u9a8c\u6570\u636e\u4e0d\u540c\u5206\u7ec4\u4e4b\u95f4\u7684\u5fae\u751f\u7269\u7ec4\u6210\u548c\u529f\u80fd\u6709\u5dee\u5f02\uff0c%GC\u8fd9\u4e2a\u503c\u5927\u6982\u7387\u4e5f\u662f\u6709\u5dee\u5f02\u7684\u3002<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">\u5de6\u8fb9summary\u91cc\u9762\u7eff\u8272\u7684\u52fe\u662f\u5408\u683c\uff0c\u8b66\u544a\u662f\u9ec4\u8272\u53f9\u53f7\uff0c\u4e0d\u5408\u683c\u662f\u7ea2\u53c9\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u63a5\u4e0b\u6765\u5c0f\u82b1\u5c31\u4ece\u4e0a\u5230\u4e0b\u4f9d\u6b21\u4e3a\u5927\u5bb6\u5c55\u793a\u548c\u5206\u6790\u7ed3\u679c\u56fe\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7550403225806451\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_073FCvfn4qULVKYxSqoX2rwszKe8TQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZZ1lrHwicfSF2w0G87tDFBO4jTHfBbRbNPxoCsgh4BJKl0b073FCvfn4qULVKYxSqoX2rwszKe8TQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"992\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 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powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7533960292580982\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-04_\u300c\u8f6c\u300d\u5b8f\u57fa\u56e0\u7ec4\u8d28\u63a7\u8fc7\u7a0b\u4e2dFastQC\u4f7f\u7528\u53ca\u7ed3\u679c\u89e3\u8bfb\/5_4bzNtB5LcIDBdNZdibSZjibP4JdnTw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZZ1lrHwicfSF2w0G87tDFBO4CXicM8JIg9vlC7heZsfVial4VZp4bzNtB5LcIDBdNZdibSZjibP4JdnTw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"957\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: 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onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZZ1lrHwicfSF2w0G87tDFBOa9hwBh9pyzWrDQDwS9d2bNyz4zevvQUjUut8HFhvWyZp1jpFbhmkNQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"955\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u4e2a\u56fe\u662f\u663e\u793areads\u6bcf\u4e2a\u4f4d\u7f6e\u51fa\u73b0N\u7684\u6bd4\u7387\u3002\u5c0f\u82b1\u4e3a\u5927\u5bb6\u89e3\u91ca\u4e00\u4e0bN\u662f\u4ec0\u4e48\uff1a\u5f53\u51fa\u73b0\u6d4b\u5e8f\u4eea\u4e0d\u80fd\u5206\u8fa8\u7684\u78b1\u57fa\u65f6\u4f1a\u4ea7\u751fN\uff0c\u56e0\u6b64\u8fc7\u591a\u7684N\u5c31\u4ee3\u8868\u6211\u4eec\u7684\u6d4b\u5e8f\u6570\u636e\u8d28\u91cf\u5e76\u4e0d\u9ad8\u3002<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7458333333333333\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-04_\u300c\u8f6c\u300d\u5b8f\u57fa\u56e0\u7ec4\u8d28\u63a7\u8fc7\u7a0b\u4e2dFastQC\u4f7f\u7528\u53ca\u7ed3\u679c\u89e3\u8bfb\/7_3B3LnicyUHgLa1KRPmdhaCZiaNuE6g.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZZ1lrHwicfSF2w0G87tDFBOjvlaMbNvCfXjajeFiaAmNWI8KGo3B3LnicyUHgLa1KRPmdhaCZiaNuE6g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"960\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u4e2a\u56fe\u662freads\u957f\u5ea6\u7684\u5206\u5e03\uff0c\u8d28\u91cf\u597d\u7684\u6570\u636e\u5f80\u5f80reads\u7684\u957f\u5ea6\u90fd\u96c6\u4e2d\u5206\u5e03\u5728\u4e00\u4e2a\u5730\u65b9\u3002<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7502601456815817\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_Qicea8BQnRYK7d7TAhm0BTLvzwnb5w.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZZ1lrHwicfSF2w0G87tDFBOKqQdxic0SI0a0AJLqeXe9IoATLQicea8BQnRYK7d7TAhm0BTLvzwnb5w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"961\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u4e2a\u56fe\u662f\u7edf\u8ba1\u5e8f\u5217\u5b8c\u5168\u4e00\u81f4\u7684reads\u7684\u9891\u7387\uff0c\u6a2a\u5750\u6807\u662fduplication\u7684\u6b21\u6570\uff0c\u7eb5\u5750\u6807\u662fduplicated reads\u7684\u6570\u76ee\uff0c\u4ee5unique reads\u7684\u603b\u6570\u4f5c\u4e3a100%\u3002<\/p>\n<p style=\"text-indent: 2em;text-wrap: wrap;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.2411467116357504\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-04_\u300c\u8f6c\u300d\u5b8f\u57fa\u56e0\u7ec4\u8d28\u63a7\u8fc7\u7a0b\u4e2dFastQC\u4f7f\u7528\u53ca\u7ed3\u679c\u89e3\u8bfb\/9_PosOyCJMA1qy8klZnR7QLx7vTr0PBw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZZ1lrHwicfSF2w0G87tDFBOiaEwuyK4KvOSWDks6fVnbFKzwPosOyCJMA1qy8klZnR7QLx7vTr0PBw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"593\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u4e2a\u7ed3\u679c\u8bf4\u660e\u6211\u4eec\u6570\u636e\u4e2d\u6ca1\u6709over-represented\u7684\u5e8f\u5217\uff0c\u5373\u6ca1\u6709\u67d0\u4e2a\u5927\u91cf\u51fa\u73b0\u7684\u5e8f\u5217\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7549738219895288\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-04_\u300c\u8f6c\u300d\u5b8f\u57fa\u56e0\u7ec4\u8d28\u63a7\u8fc7\u7a0b\u4e2dFastQC\u4f7f\u7528\u53ca\u7ed3\u679c\u89e3\u8bfb\/10_mk3ibDa3n1ibRfRrc2B4gL6NDGhmQQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZZ1lrHwicfSF2w0G87tDFBOC32M0ryqXbWENAT33n2NXQUJkemk3ibDa3n1ibRfRrc2B4gL6NDGhmQQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"955\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u4e2a\u56fe\u663e\u793a\u6211\u4eec\u7684\u5e8f\u5217\u4e2d\u662f\u5426\u6709\u63a5\u5934\uff0c\u5982\u679c\u6709\u5c31\u5fc5\u987b\u8981\u53bb\u9664\uff0c\u6a2a\u8f74\u8868\u793a\u78b1\u57fa\u4f4d\u7f6e\uff0c\u7eb5\u8f74\u8868\u793a\u63a5\u5934\u6240\u5360\u7684\u767e\u5206\u6bd4\u3002<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 98%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;border-style: solid;border-width: 2px;border-color: rgb(160, 160, 160);padding: 27px;\">\n<section style=\"text-align: justify;line-height: 1.6;letter-spacing: 0px;padding-right: 6px;padding-left: 6px;\" powered-by=\"xiumi.us\">\n<p>\u597d\u5566\uff0c\u8fd9\u5c31\u662fFastQC\u7684\u4f7f\u7528\u6559\u7a0b\u548c\u7ed3\u679c\u89e3\u8bfb\u5566\uff0c\u540e\u7eed\u5c0f\u82b1\u4f1a\u4e3a\u5927\u5bb6\u5e26\u6765\u8d28\u91cf\u63a7\u5236\u3001\u9002\u914d\u4f53\u4fee\u526a\u3001NGS\u5f15\u7269\u4fee\u526a\u7b49\u8d28\u63a7\u8f6f\u4ef6\uff0c\u5e0c\u671b\u5927\u5bb6\u80fd\u591f\u8ddf\u7740\u5c0f\u82b1\u5b66\u4e60\u66f4\u591a\u5b8f\u57fa\u56e0\u7ec4\u7684\u77e5\u8bc6\u3002<\/p>\n<p><br 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