{"id":54995,"date":"2024-06-16T16:08:40","date_gmt":"2024-06-16T08:08:40","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=54995"},"modified":"2024-06-16T16:08:40","modified_gmt":"2024-06-16T08:08:40","slug":"rna-seq%e5%b7%ae%e5%bc%82%e5%88%86%e6%9e%90%e5%ae%9e%e6%88%98%e6%8c%87%e5%8d%97%ef%bc%9a%e5%88%a9%e7%94%a8deseq2-r%e5%8c%85%e7%b2%be%e5%87%86%e8%a7%a3%e6%9e%90%e5%b7%ae%e5%bc%82%e8%a1%a8%e8%be%be","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=54995","title":{"rendered":"RNA-seq\u5dee\u5f02\u5206\u6790\u5b9e\u6218\u6307\u5357\uff1a\u5229\u7528DESeq2 R\u5305\u7cbe\u51c6\u89e3\u6790\u5dee\u5f02\u8868\u8fbe\u5e76\u53ef\u89c6\u5316\u57fa\u56e0\u8c03\u63a7\uff01"},"content":{"rendered":"


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rel=\"noopener\">#\u751f\u4fe1\u5b9e\u64cd<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=3138206666342268940#wechat_redirect\" title=\"\u751f\u4fe1\u5c0f\u767d\u76f8\u5173\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u5c0f\u767d\u76f8\u5173<\/a><\/p>\n<\/div>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100015224\" data-ratio=\"0.2222222222222222\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_r0Gd7iaumIGMiaia97aHFV15nrpdzw-103.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxQW8Zek7Noib2uBhB3ZE3yMxI2Hfic7cGTdPYiacicg47jficdr5BVr0Gd7iaumIGMiaia97aHFV15nrpdzw\/640?wx_fmt=png&from=appmsg'\" data-type=\"png\" data-w=\"900\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;\">\u5f53\u6d89\u53ca\u5230RNA-seq\u5dee\u5f02\u5206\u6790\u65f6\uff0c\u4f60\u662f\u5426\u66fe\u7ecf\u611f\u5230\u56f0\u60d1\u6216\u65e0\u4ece\u4e0b\u624b\uff1f\u672c\u671f\uff0c\u5c0f\u679c\u5c06\u5e26\u5c0f\u4f19\u4f34\u4eec\u63ed\u5f00RNA-seq\u5206\u6790\u7684\u795e\u79d8\u9762\u7eb1\uff01RNA-seq\u6280\u672f\u4e3a\u6211\u4eec\u6253\u5f00\u4e86\u4e00\u4e2a\u5168\u65b0\u7684\u7a97\u53e3\uff0c\u8ba9\u6211\u4eec\u80fd\u591f\u6df1\u5165\u4e86\u89e3\u57fa\u56e0\u8868\u8fbe\u7684\u5965\u79d8\u3002\u800c\u5728\u8fd9\u4e2a\u63a2\u7d22\u8fc7\u7a0b\u4e2d\uff0cDESeq2 R\u5305\u6210\u4e3a\u4e86\u6211\u4eec\u4e0d\u53ef\u6216\u7f3a\u7684\u5f97\u529b\u52a9\u624b\u3002\u51ed\u501f\u5176\u5f3a\u5927\u7684\u7edf\u8ba1\u529f\u80fd\u548c\u7cbe\u51c6\u7684\u5206\u6790\u80fd\u529b\uff0c\u6211\u4eec\u53ef\u4ee5\u7cbe\u51c6\u89e3\u6790RNA-seq\u6570\u636e\u4e2d\u7684\u5dee\u5f02\u8868\u8fbe\u3002\u4e0d\u4ec5\u5982\u6b64\uff0c\u901a\u8fc7\u53ef\u89c6\u5316\u624b\u6bb5\uff0c\u6211\u4eec\u80fd\u591f\u76f4\u89c2\u5730\u6d1e\u5bdf\u57fa\u56e0\u8c03\u63a7\uff0c\u5e76\u53d1\u73b0\u9690\u85cf\u5728\u6570\u636e\u80cc\u540e\u7684\u751f\u7269\u5b66\u610f\u4e49\u3002\u65e0\u8bba\u662f\u57fa\u7840\u7814\u7a76\u8fd8\u662f\u4e34\u5e8a\u5e94\u7528\uff0c<\/span><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;\">DESeq2<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u90fd\u80fd\u4e3a\u6211\u4eec\u63d0\u4f9b\u6709\u529b\u7684\u652f\u6301\u3002\u90a3\u4e48\uff0c\u4f60\u8fd8\u5728\u7b49\u4ec0\u4e48\uff1f\u5feb\u6765\u548c\u5c0f\u679c\u4e00\u8d77\u63a2\u7d22RNA-seq\u5dee\u5f02\u5206\u6790\u7684\u65e0\u9650\u53ef\u80fd\u5427\uff01<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(255, 0, 0);font-weight: bold;\">\u540c\u65f6\u5462\uff0c\u5341\u5e74\u7684\u751f\u4fe1\u4e4b\u8def\uff0c\u5c0f\u679c\u5df2\u7ecf\u7ec3\u5c31\u4e86\u4e00\u8eab\u624e\u5b9e\u7684\u672c\u9886\uff0c\u73b0\u5728\u51c6\u5907\u7528\u8fd9\u8eab\u672c\u4e8b\u4e3a\u5c0f\u4f19\u4f34\u4eec\u670d\u52a1\u5566\uff01\u5982\u679c\u4f60\u5728\u751f\u4fe1\u5206\u6790\u4e0a\u9047\u5230\u4e86\u96be\u9898\uff0c\u90a3\u5c31\u6765\u627e\u5c0f\u679c\u5427\uff01\u5c0f\u679c\u4f1a\u7528\u81ea\u5df1\u7684\u4e13\u4e1a\u77e5\u8bc6\u548c\u6280\u80fd\uff0c\u4e3a\u4f60\u89e3\u51b3\u56f0\u6270\uff0c\u52a9\u4f60\u4e00\u81c2\u4e4b\u529b\u3002\u671f\u5f85\u4f60\u7684\u8054\u7cfb\u54e6~<\/span><\/span><\/p>\n<h2 style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 173%;font-family: Cambria;font-variant: normal;text-transform: none;\">\u4e00\u3001DESeq2\u5305\u7b80\u8981\u8bf4\u660e<\/span><br \/>\n  <\/span><\/h2>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(255, 192, 0);font-weight: bold;font-style: italic;\">DESeq2\u5305<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u662f\u4e00\u4e2a\u4e3aRNA-seq\u6570\u636e\u4e2d\u7684\u9ad8\u7ef4\u8ba1\u6570\u6570\u636e\u8bbe\u8ba1\u7684R\u8bed\u8a00\u5305\uff0c\u4e13\u6ce8\u4e8e\u6570\u636e\u7684\u5f52\u4e00\u5316\u3001\u53ef\u89c6\u5316\u548c\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u3002\u5176\u4e3b\u8981\u529f\u80fd\u662f\u5229\u7528<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(255, 192, 0);font-weight: bold;font-style: italic;\">\u7ecf\u9a8c\u8d1d\u53f6\u65af\u65b9\u6cd5\uff08empirical Bayes techniques\uff09<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u6765\u4f30\u8ba1\u5bf9\u6570\u500d\u6570\u53d8\u5316\uff08log2foldchange\uff09\u548c\u79bb\u5dee\u7684\u5148\u9a8c\u503c\uff0c\u5e76\u8ba1\u7b97\u8fd9\u4e9b\u7edf\u8ba1\u91cf\u7684\u540e\u9a8c\u503c\u3002\u8fd9\u4e5f\u5c31\u662f\u4e3a\u4ec0\u4e48DESeq2\u80fd\u591f\u5728RNA-seq\u6570\u636e\u4e2d\u7cbe\u786e\u5730\u63a2\u6d4b\u57fa\u56e0\u5dee\u5f02\u8868\u8fbe\u7684\u539f\u56e0\u5566~<\/span>    <\/span><o:page><\/o:page><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: justify;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Wingdings;font-variant: normal;text-transform: none;color: windowtext;font-weight: bold;font-style: italic;\">l<\/span><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(255, 192, 0);font-weight: bold;font-style: italic;\">DESeq2\u5305\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u6b65\u9aa4\uff1a<\/span><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: justify;margin-left: 46pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Wingdings;font-variant: normal;text-transform: none;\">\u00d8<\/span><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;\">DESeq2\u9996\u5148\u5bf9\u539f\u59cbreads\u8fdb\u884c\u5efa\u6a21\uff0c\u4f7f\u7528\u6807\u51c6\u5316\u56e0\u5b50\uff08scale factor\uff09\u6765\u89e3\u91ca\u5e93\u6df1\u5ea6\u7684\u5dee\u5f02\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: justify;margin-left: 46pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Wingdings;font-variant: normal;text-transform: none;\">\u00d8<\/span><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;\">DESeq2\u4f30\u8ba1\u57fa\u56e0\u7684\u79bb\u6563\u5ea6\uff0c\u5e76\u7f29\u5c0f\u8fd9\u4e9b\u4f30\u8ba1\u503c\u4ee5\u751f\u6210\u66f4\u51c6\u786e\u7684\u79bb\u6563\u5ea6\u4f30\u8ba1\uff0c\u4ece\u800c\u5bf9reads count\u8fdb\u884c\u5efa\u6a21\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: justify;margin-left: 46pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Wingdings;font-variant: normal;text-transform: none;\">\u00d8<\/span><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;\">DESeq2\u62df\u5408\u8d1f\u4e8c\u9879\u5206\u5e03\u7684\u6a21\u578b\uff0c\u5e76\u4f7f\u7528Wald\u68c0\u9a8c\u6216\u4f3c\u7136\u6bd4\u68c0\u9a8c\u8fdb\u884c\u5047\u8bbe\u68c0\u9a8c\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: justify;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Wingdings;font-variant: normal;text-transform: none;\">\u00b2<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(0, 112, 192);font-weight: bold;font-style: italic;\">\u5c0f\u679c\u63d0\u793a\uff1a<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u5728\u8fdb\u884c\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u4e4b\u524d\uff0c\u9700\u8981\u51c6\u5907\u597d\u8f93\u5165\u6570\u636e\u3002DESeq2\u5305\u4f7f\u7528\u57fa\u4e8e\u8ba1\u6570\u7684RNA-seq\u6570\u636e\u4f5c\u4e3a\u8f93\u5165\u3002\u901a\u5e38\uff0c\u6211\u4eec\u9700\u8981\u5c06\u539f\u59cb\u6d4b\u5e8f\u6570\u636e\u6620\u5c04\u5230\u57fa\u56e0\u6216\u8f6c\u5f55\u672c\u6c34\u5e73\uff0c\u5e76\u8ba1\u7b97\u6bcf\u4e2a\u57fa\u56e0\u6216\u8f6c\u5f55\u672c\u7684\u8bfb\u6570\u3002<\/span><\/span><\/p>\n<h2 style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 173%;font-family: Cambria;font-variant: normal;text-transform: none;\">\u4e8c\u3001<\/span><span style=\"line-height: 173%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u4ee3\u7801\u5b9e\u64cd<\/span><br \/>\n  <\/span><\/h2>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u5c0f\u679c\u8fd9\u91cc\u5c06\u7528\u4e00\u6bb5\u4ee3\u7801\u548c\u5c0f\u4f19\u4f34\u4eec\u4e00\u8d77\u5b66\u4e60DESeq2\u5305\u8fdb\u884cRNA-seq\u5dee\u5f02\u5206\u6790\u7684\u4e00\u4e2a\u5178\u578b\u6d41\u7a0b\u3002<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(255, 0, 0);font-weight: bold;\">\u672c\u6b21\u4ecb\u7ecd\u7684R\u5305\u64cd\u4f5c\u4f1a\u5360\u7528\u5185\u5b58\u8f83\u5927\uff0c\u5c0f\u679c\u5efa\u8bae\u4f7f\u7528\u670d\u52a1\u5668\uff0c\u6b22\u8fce\u5c0f\u4f19\u4f34\u4eec\u8054\u7cfb\u5c0f\u679c\u79df\u8d41\u6027\u4ef7\u6bd4\u5c45\u9ad8\u7684\u670d\u52a1\u5668\uff01<\/span><\/span><\/p>\n<section style=\"font-size: 15px;padding-right: 2px;padding-left: 2px;margin-bottom: 0px;\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;\">\n<section style=\"display: flex;width: 100%;flex-flow: column;\" powered-by=\"xiumi.us\">\n<section style=\"z-index: 1;transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;transform: translate3d(4px, 0px, 0px) rotateX(180deg);margin-bottom: -2px;\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 10px;height: 14px;vertical-align: top;overflow: hidden;background-color: rgb(91, 91, 91);border-style: solid;border-width: 2px;border-color: rgb(255, 255, 255);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 10px;height: 14px;vertical-align: top;overflow: hidden;background-color: rgb(91, 91, 91);border-style: solid;border-width: 2px;border-color: rgb(255, 255, 255);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(194, 209, 255);\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row;margin-top: -4px;margin-bottom: 5px;transform: translate3d(5px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(193, 209, 255);padding: 10px 20px;box-shadow: rgb(255, 255, 255) -2px 2px 0px;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;align-self: center;flex: 0 0 auto;width: 100%;height: auto;\">\n<section style=\"text-align: center;color: rgb(0, 0, 0);\" powered-by=\"xiumi.us\">\n<p><strong>\u516c\u4f17\u53f7\u540e\u53f0\u56de\u590d\u201c111\u201d<\/strong><\/p>\n<p><strong>\u9886\u53d6\u672c\u7bc7\u4ee3\u7801\u3001\u57fa\u56e0\u96c6\u6216\u793a\u4f8b\u6570\u636e\u7b49\u6587\u4ef6<\/strong><\/p>\n<p><strong>\u6587\u4ef6\u7f16\u53f7\uff1a240520<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-wrap: wrap;\" powered-by=\"xiumi.us\"><span style=\"color: rgb(171, 25, 66);\"><strong>\u9700\u8981\u79df\u8d41\u670d\u52a1\u5668\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u626b\u7801\u6dfb\u52a0\u5c0f\u679c\uff0c\u6b64\u5916\u5c0f\u679c\u8fd8\u63d0\u4f9b\u751f\u4fe1\u5206\u6790\uff0c\u601d\u8def\u8bbe\u8ba1\uff0c\u6587\u732e\u590d\u73b0\u7b49\uff0c\u6709\u9700\u8981\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u6765\u64a9~<\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100018428\" data-ratio=\"1\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_f6PG40Qrdu5e9B1iaIMItPiajABYWA-43.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxqaqPbQprPd4vw8Ytp0cprbzXiakMDdFriaB6B59ibhlBCrNyQFf6PG40Qrdu5e9B1iaIMItPiajABYWA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"430\" style=\"width: 170px;height: 170px;\" data-recalc-dims=\"1\" \/><span style=\"background-color: rgb(255, 255, 255);color: rgb(255, 0, 0);font-family: \u5b8b\u4f53;font-size: 16px;font-weight: bold;letter-spacing: 0.034em;text-indent: 0em;text-align: justify;\"><\/span><\/p>\n<\/section>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: justify;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Wingdings;font-variant: normal;text-transform: none;\">l<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-weight: bold;\">\u5305\u7684\u5b89\u88c5\u4e0e\u52a0\u8f7d\uff1a<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u9996\u5148\uff0c\u68c0\u67e5\u662f\u5426\u5b89\u88c5\u4e86BiocManager\uff0c\u5982\u679c\u6ca1\u6709\uff0c\u5219\u8fdb\u884c\u5b89\u88c5\u3002<\/span><\/span><span style=\"background-color: rgb(255, 255, 255);color: rgb(0, 176, 80);font-family: \u5b8b\u4f53;font-size: 16px;letter-spacing: 0.4pt;text-indent: 0em;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span> (!requireNamespace(<span class=\"code-snippet__string\">\"BiocManager\"<\/span>, quietly = <span class=\"code-snippet__keyword\">TRUE<\/span>)) {<\/span><\/code><code><span class=\"code-snippet_outer\"> install.packages(<span class=\"code-snippet__string\">\"BiocManager\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">} <\/span><\/code><code><span class=\"code-snippet_outer\">BiocManager::install(<span class=\"code-snippet__string\">\"DESeq2\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">library(DESeq2)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);letter-spacing: 0.4pt;background: rgb(255, 255, 255);font-size: 16px;\"><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: justify;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Wingdings;font-variant: normal;text-transform: none;\">l<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-weight: bold;\">\u8fdb\u884c\u6a21\u62df\u6570\u636e\u96c6\u7684\u751f\u6210\uff1a<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u5c0f\u679c\u8fd9\u91cc\u4f7f\u7528\u4e86set.seed\u786e\u4fdd\u6a21\u62df\u7ed3\u679c\u7684\u53ef\u91cd\u590d\u6027\u3002\u5b9a\u4e49\u6761\u4ef6\u548c\u6837\u672c\u3002\u4f7f\u7528rpois\u751f\u6210\u77e9\u9635\uff0c\u6a21\u62df\u4e86\u57fa\u56e0\u5728\u6837\u672c\u4e2d\u7684\u8868\u8fbe\u6c34\u5e73\u3002<\/span><\/span><span style=\"background-color: rgb(255, 255, 255);color: rgb(0, 176, 80);font-family: \u5b8b\u4f53;font-size: 16px;letter-spacing: 0.4pt;text-indent: 0em;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"properties\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">set.seed(123)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">conditions<\/span> <span class=\"code-snippet__string\"><- c(\"Condition1\", \"Condition2\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">samples<\/span> <span class=\"code-snippet__string\"><- paste0(\"Sample_\", 1:6)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">counts<\/span> <span class=\"code-snippet__string\"><- matrix(rpois(60, 1000), ncol = 6)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">colData<\/span> <span class=\"code-snippet__string\"><- data.frame(<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__attr\">sample<\/span> = <span class=\"code-snippet__string\">samples,<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__attr\">condition<\/span> = <span class=\"code-snippet__string\">factor(rep(conditions, each = 3))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">)<\/span> <span class=\"code-snippet__string\"><\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><\/span><o:page><\/o:page><\/p>\n<p style=\"text-align: center;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);letter-spacing: 0.4pt;background: rgb(255, 255, 255);font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018419\" data-ratio=\"0.6588693957115009\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_ZfKNibSkqGaibrfDUoE6QvvnV6O4Ug.png?resize=477%2C314\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyZYWxkcMjUGDz4aMnt6SC3micGeNm9r05huubCCaS5QiaM0lJvZfKNibSkqGaibrfDUoE6QvvnV6O4Ug\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"513\" height=\"314 .739990234375\" width=\"477 .69000244140625\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"text-align: center;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);letter-spacing: 0.4pt;background: rgb(255, 255, 255);font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018418\" data-ratio=\"1.021505376344086\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_iavqeCWaz2EicknfRDwgcVKblPGVEw.png?resize=242%2C247\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyZYWxkcMjUGDz4aMnt6SC3LpamQMywWgKGT7Sa0BMkgxAawQiavqeCWaz2EicknfRDwgcVKblPGVEw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"279\" height=\"247 .87997436523438\" width=\"242 .66000366210938\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: justify;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Wingdings;font-variant: normal;text-transform: none;\">l<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-weight: bold;\">\u521b\u5efaDESeq2\u6570\u636e\u96c6\u5bf9\u8c61\uff1a<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u7528DESeqDataSetFromMatrix\u521b\u5efaDESeq2\u6570\u636e\u96c6\u5bf9\u8c61dds\uff0c\u5176\u4e2d\u5305\u542b\u4e86\u8ba1\u6570\u6570\u636e\u548c\u6837\u672c\u4fe1\u606f\u3002<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code><span class=\"code-snippet_outer\">dds <span class=\"code-snippet__tag\"><<span class=\"code-snippet__name\">-<\/span> <span class=\"code-snippet__attr\">DESeqDataSetFromMatrix<\/span>(<span class=\"code-snippet__attr\">countData<\/span> = <span class=\"code-snippet__string\">counts,<\/span> <span class=\"code-snippet__attr\">colData<\/span> = <span class=\"code-snippet__string\">colData,<\/span> <span class=\"code-snippet__attr\">design<\/span> = <span class=\"code-snippet__string\">~<\/span> <span class=\"code-snippet__attr\">condition<\/span>)<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);letter-spacing: 0.4pt;background: rgb(255, 255, 255);font-size: 16px;\"><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: justify;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Wingdings;font-variant: normal;text-transform: none;font-weight: bold;\">l<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-weight: bold;\">\u6570\u636e\u9884\u5904\u7406\uff1a<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u5148\u7528estimateSizeFactors\u5bf9\u6570\u636e\u8fdb\u884c\u6807\u51c6\u5316\uff0c\u4ee5\u6d88\u9664\u6837\u672c\u4e4b\u95f4\u7684\u5927\u5c0f\u5dee\u5f02\uff0c\u7136\u540e\u7528estimateDispersionsGeneEst\u8fdb\u884c\u57fa\u56e0\u7ea7\u522b\u7684\u79bb\u6563\u5ea6\u4f30\u8ba1\uff0c\u5e76\u66f4\u65b0dds\u5bf9\u8c61\u3002<\/span>    <\/span><o:page><\/o:page><\/p>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);letter-spacing: 0.4pt;background: rgb(255, 255, 255);font-size: 16px;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">dds<\/span> <- estimateSizeFactors(dds)<\/span><\/code><code><span class=\"code-snippet_outer\">dds <- estimateDispersionsGeneEst(dds)<\/span><\/code><code><span class=\"code-snippet_outer\">dispersions(dds) <- mcols(dds)<span class=\"code-snippet__variable\">$dispGeneEst<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);letter-spacing: 0.4pt;background: rgb(255, 255, 255);font-size: 16px;\"><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: justify;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Wingdings;font-variant: normal;text-transform: none;font-weight: bold;\">l<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-weight: bold;\">\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff1a<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u7528nbinomWaldTest\u62df\u5408\u8d1f\u4e8c\u9879\u6a21\u578b\u5e76\u8fdb\u884c\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u3002<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">dds<\/span> <- nbinomWaldTest(dds)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);letter-spacing: 0.4pt;background: rgb(255, 255, 255);font-size: 16px;\"><\/span><\/p>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;font-weight: bold;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018416\" data-ratio=\"0.401468788249694\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_X8ahRXM5BqfgeNxlE2HTh04GZeGpOg.png?resize=514%2C206\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyZYWxkcMjUGDz4aMnt6SC3tKOqlI5vU1xLupgkDd15BOiczX8ahRXM5BqfgeNxlE2HTh04GZeGpOg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"817\" height=\"206 .62997436523438\" width=\"514 .6300048828125\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: justify;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Wingdings;font-variant: normal;text-transform: none;font-weight: bold;\">l<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-weight: bold;\">\u79bb\u6563\u5ea6\u5206\u5e03\u7684\u53ef\u89c6\u5316\uff1a<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u7528plotDispEsts\u67e5\u770b\u6570\u636e\u7684\u79bb\u6563\u5ea6\u5206\u5e03\uff0c\u8fd9\u6709\u52a9\u4e8e\u5224\u65ad\u6a21\u578b\u7684\u9002\u7528\u6027\u3002<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"javascript\"><code><span class=\"code-snippet_outer\">plotDispEsts(dds, fitType=<span class=\"code-snippet__string\">\"local\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);letter-spacing: 0.4pt;background: rgb(255, 255, 255);font-size: 16px;\"><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: center;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;font-weight: bold;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018417\" data-ratio=\"0.8597475455820477\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_VvyhmrmowMBibh9s9twO7ey9QNLLXw.png?resize=358%2C308\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyZYWxkcMjUGDz4aMnt6SC3tmZb8xjWbSVPYbPC7NGrH876bVvyhmrmowMBibh9s9twO7ey9QNLLXw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"713\" height=\"308 .3299560546875\" width=\"358 .6300048828125\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: justify;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Wingdings;font-variant: normal;text-transform: none;font-weight: bold;\">l<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-weight: bold;\">\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7ed3\u679c\u7684\u83b7\u53d6\u4e0e\u8fc7\u6ee4\uff1a<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u4f7f\u7528results\u83b7\u53d6\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u7684\u7ed3\u679c\u540e\u901a\u8fc7subset\u8fc7\u6ee4\u51faFDR\u6821\u6b63\u540eP\u503c\u5c0f\u4e8e0.05\u7684\u663e\u8457\u6027\u57fa\u56e0\u3002<\/span>    <\/span><span style=\"background-color: rgb(255, 255, 255);color: rgb(0, 176, 80);font-family: \u5b8b\u4f53;font-size: 16px;letter-spacing: 0.4pt;text-indent: 0em;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"properties\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">res<\/span> <span class=\"code-snippet__string\"><- results(dds)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">print(res)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">resSignificant<\/span> <span class=\"code-snippet__string\"><- subset(res, padj < 0.05)<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"text-indent: 0em;font-size: 16px;letter-spacing: 0.034em;line-height: 150%;font-family: Wingdings;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-variant-position: normal;\">l<\/span><span style=\"text-indent: 0em;font-size: 16px;letter-spacing: 0.034em;line-height: 150%;font-family: \u5b8b\u4f53;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-variant-position: normal;font-weight: bold;\">\u6570\u636e\u53ef\u89c6\u5316\uff08\u68c0\u67e5\u6570\u636e\u8d28\u91cf\uff09\uff1a<\/span><span style=\"text-indent: 0em;font-size: 16px;letter-spacing: 0.034em;line-height: 150%;font-family: \u5b8b\u4f53;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-variant-position: normal;\">\u5c0f\u679c\u8fd9\u91cc\u8fdb\u884c\u4e86\u591a\u79cd\u53ef\u89c6\u5316~plotMA\u7ed8\u5236\u706b\u5c71\u56fe\uff0c\u5c55\u793a\u57fa\u56e0\u7684log2 fold change\u548c\u663e\u8457\u6027\u6c34\u5e73\uff1bboxplot\u7ed8\u5236\u7bb1\u7ebf\u56fe\uff0c\u68c0\u67e5\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u4e2d\u7684\u5f02\u5e38\u503c\uff1bvioplot\u7ed8\u5236\u5c0f\u63d0\u7434\u56fe\uff0c\u5c55\u793a\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u7684\u5206\u5e03\uff1bstripchart\u7ed8\u5236\u70b9\u56fe\uff0c\u5c55\u793a\u6bcf\u4e2a\u6837\u672c\u4e2d\u6bcf\u4e2a\u57fa\u56e0\u7684\u8868\u8fbe\u91cf\uff1bheatmap3\u7ed8\u5236\u70ed\u56fe\uff0c\u5c55\u793a\u57fa\u56e0\u8868\u8fbe\u91cf\u7684\u5206\u5e03\uff1bpairs\u7ed8\u5236\u6563\u70b9\u56fe\u77e9\u9635\uff0c\u5c55\u793a\u57fa\u56e0\u8868\u8fbe\u91cf\u4e4b\u95f4\u7684\u6210\u5bf9\u5173\u7cfb\u3002<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);letter-spacing: 0.4pt;background: rgb(255, 255, 255);font-size: 16px;\"><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: justify;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\"><br \/><\/span><\/span><\/p>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);letter-spacing: 0.4pt;background: rgb(255, 255, 255);font-size: 16px;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"objectivec\"><code><span class=\"code-snippet_outer\">plotMA(res, main=<span class=\"code-snippet__string\">\"Volcano Plot\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">boxplot(assay(dds), las = <span class=\"code-snippet__number\">2<\/span>, col = <span class=\"code-snippet__string\">\"skyblue\"<\/span>, pch = <span class=\"code-snippet__number\">20<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> main = <span class=\"code-snippet__string\">\"Boxplot of Gene Expression Data\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span> (!requireNamespace(<span class=\"code-snippet__string\">\"vioplot\"<\/span>, quietly = <span class=\"code-snippet__literal\">TRUE<\/span>)) {<\/span><\/code><code><span class=\"code-snippet_outer\"> install.packages(<span class=\"code-snippet__string\">\"vioplot\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">library(vioplot)<\/span><\/code><code><span class=\"code-snippet_outer\">vioplot(assay(dds), col = <span class=\"code-snippet__string\">\"lightgreen\"<\/span>, pch = <span class=\"code-snippet__number\">20<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> main = <span class=\"code-snippet__string\">\"vioplot of Gene Expression Data\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">stripchart(assay(dds), method = <span class=\"code-snippet__string\">\"jitter\"<\/span>, pch = <span class=\"code-snippet__number\">20<\/span>, col = <span class=\"code-snippet__string\">\"yellow\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> main = <span class=\"code-snippet__string\">\"Stripchart of Gene Expression Data\"<\/span>) <\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"heatmap3\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">library(heatmap3)<\/span><\/code><code><span class=\"code-snippet_outer\">heatmap3(assay(dds), scale = <span class=\"code-snippet__string\">\"row\"<\/span>, col = heat.colors(<span class=\"code-snippet__number\">100<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> main = <span class=\"code-snippet__string\">\"Heatmap of Gene Expression Data\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">pairs(assay(dds), col = <span class=\"code-snippet__string\">\"purple\"<\/span>, pch = <span class=\"code-snippet__number\">20<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> main = <span class=\"code-snippet__string\">\"Scatterplot Matrix of Gene Expression Data\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);letter-spacing: 0.4pt;background: rgb(255, 255, 255);font-size: 16px;\"><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: center;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;font-weight: bold;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018415\" data-ratio=\"0.8597475455820477\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_MVpAEzur7gl5y2JSD28yb32f2jnE8Q.png?resize=349%2C300\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyZYWxkcMjUGDz4aMnt6SC3A3KibZUjAmdZeQLv0CsGMmSWtMVpAEzur7gl5y2JSD28yb32f2jnE8Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"713\" height=\"300 .3500061035156\" width=\"349 .3399658203125\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: center;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018423\" data-ratio=\"0.8597475455820477\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_Fllxco1jKk6AoWicib0v6giaiaibKA.png?resize=341%2C293\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyZYWxkcMjUGDz4aMnt6SC3ffhM60yBUbxcdDhwFUx6udTMbVeosFllxco1jKk6AoWicib0v6giaiaibKA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"713\" height=\"293 .60003662109375\" width=\"341 .489990234375\" data-recalc-dims=\"1\" \/><\/span><span style=\"font-size: 16px;\">    <\/span><o:page><\/o:page><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: center;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018421\" data-ratio=\"0.8597475455820477\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_ibb5cuCCzEWVwPFccuvPZ8adRrATtA.png?resize=430%2C370\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyZYWxkcMjUGDz4aMnt6SC3ZzFo5ENPeO7FY7hiccibbWdm2gOibb5cuCCzEWVwPFccuvPZ8adRrATtA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"713\" height=\"370 .3500061035156\" width=\"430 .76995849609375\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: center;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018420\" data-ratio=\"0.8597475455820477\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_4XHianPrqAmq9c0zw6TkHGFdJMrMmQ.png?resize=390%2C335\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyZYWxkcMjUGDz4aMnt6SC3e7MPvicC6tyYGPJqv7bYPSIKkj4XHianPrqAmq9c0zw6TkHGFdJMrMmQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"713\" height=\"335 .32000732421875\" width=\"390 .01995849609375\" data-recalc-dims=\"1\" \/><\/span><span style=\"font-size: 16px;\">    <\/span><o:page><\/o:page><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: center;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018424\" data-ratio=\"0.8597475455820477\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_uuEuoibo8ic6SvGrBItqkbKznI76oA.png?resize=381%2C327\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyZYWxkcMjUGDz4aMnt6SC3CI7KGC6T8QqbHl8Na8jD4MMBKQuuEuoibo8ic6SvGrBItqkbKznI76oA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"713\" height=\"327 .7900085449219\" width=\"381 .260009765625\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: center;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018422\" data-ratio=\"0.8597475455820477\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/12_VRqLhOO9WkicLM1hQYTmUiaicHzF0g.png?resize=387%2C333\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyZYWxkcMjUGDz4aMnt6SC3YnFA3x53dBLB3A4FuolHTFKjHSDVRqLhOO9WkicLM1hQYTmUiaicHzF0g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"713\" height=\"333 .0999755859375\" width=\"387 .42999267578125\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: justify;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Wingdings;font-variant: normal;text-transform: none;font-weight: bold;\">l<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-weight: bold;\">\u6837\u672c\u76f8\u5173\u6027\u5206\u6790\uff1a<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u7528dist\u51fd\u6570\u8ba1\u7b97\u6837\u672c\u95f4\u7684\u6b27\u6c0f\u8ddd\u79bb\uff0c\u5e76\u4f7f\u7528heatmap3\u7ed8\u5236\u6837\u672c\u76f8\u5173\u6027\u70ed\u56fe\u3002<\/span><\/span><span style=\"background-color: rgb(255, 255, 255);color: rgb(0, 176, 80);font-family: \u5b8b\u4f53;font-size: 16px;letter-spacing: 0.4pt;text-indent: 0em;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"javascript\"><code><span class=\"code-snippet_outer\">sampleDists <- dist(t(assay(dds)))<\/span><\/code><code><span class=\"code-snippet_outer\">sampleDistMatrix <- <span class=\"code-snippet__keyword\">as<\/span>.matrix(sampleDists)<\/span><\/code><code><span class=\"code-snippet_outer\">heatmap3(sampleDistMatrix, main=<span class=\"code-snippet__string\">\"Sample Correlation Heatmap\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;font-weight: bold;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018425\" data-ratio=\"0.8597475455820477\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/13_icricJhelb0lficYMTdq7kicZQh1Vg.png?resize=407%2C350\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyZYWxkcMjUGDz4aMnt6SC3WzqTICppBibkRPIIXLxwVeF9IKBicpicricJhelb0lficYMTdq7kicZQh1Vg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"713\" height=\"350 .4100036621094\" width=\"407 .57000732421875\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: justify;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Wingdings;font-variant: normal;text-transform: none;font-weight: bold;\">l<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-weight: bold;\">\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\uff1a<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u4f7f\u7528prcomp\u8fdb\u884cPCA\u5206\u6790\uff0c\u5e76\u901a\u8fc7biplot\u7ed8\u5236\u524d\u4e24\u4e2a\u4e3b\u6210\u5206\u7684\u4e8c\u7ef4\u6295\u5f71\uff0c\u4ee5\u5c55\u793a\u6837\u672c\u95f4\u7684\u76f8\u5173\u6027\u3002<\/span><\/span><\/p>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);letter-spacing: 0.4pt;background: rgb(255, 255, 255);font-size: 16px;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code><span class=\"code-snippet_outer\">pca_result <span class=\"code-snippet__tag\"><<span class=\"code-snippet__name\">-<\/span> <span class=\"code-snippet__attr\">prcomp<\/span>(<span class=\"code-snippet__attr\">t<\/span>(<span class=\"code-snippet__attr\">assay<\/span>(<span class=\"code-snippet__attr\">dds<\/span>)), <span class=\"code-snippet__attr\">scale.<\/span> = <span class=\"code-snippet__string\">TRUE)<\/span><\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">biplot<\/span>(<span class=\"code-snippet__attr\">pca_result<\/span>, <span class=\"code-snippet__attr\">scale<\/span> = <span class=\"code-snippet__string\">TRUE,<\/span> <span class=\"code-snippet__attr\">col<\/span> = <span class=\"code-snippet__string\">as.numeric(colData(dds)$condition),<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__attr\">pch<\/span> = <span class=\"code-snippet__string\">19,<\/span> <span class=\"code-snippet__attr\">main<\/span> = <span class=\"code-snippet__string\">\"PCA Biplot of Sample Correlation\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);letter-spacing: 0.4pt;background: rgb(255, 255, 255);font-size: 16px;\"><\/span><\/p>\n<p style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: center;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;font-weight: bold;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018426\" data-ratio=\"0.8597475455820477\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/14_hXO2cq3cXo9PicIHmRXxzSAAhgibFg.png?resize=367%2C315\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyZYWxkcMjUGDz4aMnt6SC3YVbibbJwiaiaU8nICeb7fFrd3Br5uhXO2cq3cXo9PicIHmRXxzSAAhgibFg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"713\" height=\"315 .51995849609375\" width=\"367 .0\" data-recalc-dims=\"1\" \/><\/span><span style=\"font-size: 16px;\">    <\/span><o:page><\/o:page><\/p>\n<h2 style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 173%;font-family: Cambria;font-variant: normal;text-transform: none;\">\u4e09\u3001<\/span><span style=\"line-height: 173%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u6587\u7ae0\u5c0f\u7ed3<\/span><br \/>\n  <\/span><\/h2>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-size: 16px;line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u672c\u671f\uff0c\u5c0f\u679c\u4e3a\u5c0f\u4f19\u4f34\u4eec\u8bb2\u89e3\u4e86DESeq2 R\u5305\u5728\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u5206\u6790\u4e2d\u7684\u5e94\u7528\u3002\u6211\u4eec\u6a21\u62df\u4e86\u57fa\u56e0\u8868\u8fbe\u6570\u636e\uff0c\u5e76\u5229\u7528DESeq2\u8fdb\u884c\u6570\u636e\u9884\u5904\u7406\u3001\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff0c\u5e76\u901a\u8fc7\u706b\u5c71\u56fe\u5c55\u793a\u5206\u6790\u7ed3\u679c\u3002\u6b64\u5916\uff0c\u901a\u8fc7\u7bb1\u7ebf\u56fe\u3001\u70b9\u56fe\u3001\u70ed\u56fe\u548c\u6563\u70b9\u56fe\u7b49\u591a\u79cd\u53ef\u89c6\u5316\u624b\u6bb5\uff0c\u6df1\u5165\u63a2\u7d22\u4e86\u6570\u636e\u96c6\u7684\u5185\u5728\u7ed3\u6784\u548c\u6837\u672c\u95f4\u7684\u76f8\u4e92\u5173\u7cfb\u3002\u6700\u540e\uff0c\u901a\u8fc7\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u8fdb\u4e00\u6b65\u5206\u6790\u4e86\u6837\u672c\u95f4\u7684\u76f8\u5173\u6027\u3002\u6574\u4e2a\u8fc7\u7a0b\u4e0d\u4ec5\u63ed\u793a\u4e86\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\uff0c\u8fd8\u6df1\u5165\u5206\u6790\u4e86\u5bf9\u6570\u636e\u96c6\u3002\u5feb\u6765\u548c\u5c0f\u679c\u4e00\u8d77\u5c1d\u8bd5DESeq2\uff0c\u89e3\u9501\u4f60\u7684\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u4e4b\u79d8\u5427\uff01<\/span><span style=\"font-size: 16px;line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(6, 6, 7);letter-spacing: 0.4pt;background: rgb(255, 255, 255);\">\u5e0c\u671b\u672c\u671f\u7684\u5185\u5bb9\u5bf9\u4f60\u6709\u5e2e\u52a9\uff0c\u671f\u5f85\u6211\u4eec\u4e0b\u671f\u518d\u89c1\uff01<\/span><span style=\"font-size: 16px;line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(255, 0, 0);font-weight: bold;\">\u6700\u540e\u5982\u679c\u5404\u4f4d\u5c0f\u4f19\u4f34\u4eec\u89c9\u5f97\u81ea\u5df1\u8fd0\u884c\u4ee3\u7801\u592a\u9ebb\u70e6\uff0c\u6b22\u8fce\u7528\u6211\u4eec\u7684\u4e91\u751f\u4fe1\u5c0f\u5de5\u5177\uff0c\u53ea\u8981\u8f93\u5165\u5408\u9002\u7684\u6570\u636e\u5c31\u53ef\u4ee5\u76f4\u63a5\u51fa\u60f3\u8981\u7684\u56fe\u5462\uff0c\u9644\u4e91\u751f\u4fe1\u94fe\u63a5<\/span><\/span><\/p>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-size: 16px;line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(255, 0, 0);font-weight: bold;\">\uff08http:\/\/www.biocloudservice.com\/home.html\uff09\u3002<\/span> <\/span><\/p>\n<p style=\"text-indent: 0px;margin-bottom: 0px;\"><span style=\"color: rgb(0, 122, 170);\"><strong><span style=\"color: rgb(0, 122, 170);letter-spacing: 0.578px;text-indent: 32px;text-wrap: wrap;background-color: rgb(255, 255, 255);font-size: 15px;\">\u5c0f\u679c\u8fd8<span style=\"color: rgb(0, 122, 170);background-color: rgb(255, 255, 255);font-size: 15px;letter-spacing: 0.578px;text-decoration: none solid rgba(0, 0, 0, 0.9);\">\u63d0\u4f9b\u601d\u8def\u8bbe\u8ba1\u3001\u5b9a\u5236\u751f\u4fe1\u5206\u6790\u3001\u6587\u732e\u601d\u8def\u590d\u73b0\uff1b\u6709\u9700\u8981\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u76f4\u63a5\u626b\u7801\u54a8\u8be2\u5c0f\u679c\uff0c\u7aed\u8bda\u4e3a\u60a8\u7684\u79d1\u7814\u52a9\u529b\uff01<\/span><\/span><\/strong><\/span><\/p>\n<p style=\"margin-bottom: 0px;\"><br \/><\/p>\n<section style=\"font-size: 15px;padding-right: 2px;padding-left: 2px;margin-bottom: 0px;\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;flex: 0 0 auto;height: auto;border-color: rgb(114, 114, 114);border-width: 1px;border-style: solid;box-shadow: rgb(194, 209, 255) -5px 5px 0px;align-self: flex-start;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 42.6829%;vertical-align: middle;box-shadow: rgb(43, 65, 110) 0px 0px 0px;flex: 0 0 auto;height: auto;padding-left: 10px;align-self: center;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;line-height: 0;transform: translate3d(20px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 75%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018429\" data-ratio=\"1\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/15_B2jkicacLzPjw4xMSqVvoucZjonVDA-14.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxVYfSsM0wdf9d3RpNiaTcHohxPNEVs2VFhPMukpU8lchxrvcB2jkicacLzPjw4xMSqVvoucZjonVDA\/640?wx_fmt=jpeg&from=appmsg'\" data-type=\"jpeg\" data-w=\"430\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 56.9106%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"line-height: 2;letter-spacing: 1px;color: rgb(43, 65, 110);\" powered-by=\"xiumi.us\">\n<p style=\"margin-bottom: 2px;\"><strong>\u5b9a\u5236\u751f\u4fe1\u5206\u6790<\/strong><\/p>\n<p style=\"margin-bottom: 2px;\"><strong>\u670d\u52a1\u5668\u79df\u8d41<\/strong><\/p>\n<p><strong>\u626b\u7801\u54a8\u8be2\u5c0f\u679c<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-wrap: wrap;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247497780&idx=2&sn=7eee90af6f387e4153bba13028e545a1&chksm=cf7f68f1f808e1e7dfb3a0597ef442e5c6e700ecf6ee5a035c766a4c7486c12eabd0ab218ea6&scene=21#wechat_redirect\" textvalue=\"\u4f60\u5df2\u9009\u4e2d\u4e86\u6dfb\u52a0\u94fe\u63a5\u7684\u5185\u5bb9\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018431\" data-ratio=\"0.3253333333333333\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/16_7TvAyUibd1TPhJmZfialJtv6tu2SEg-16.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxVYfSsM0wdf9d3RpNiaTcHoj9xlsoyiaBvzaezDUFAicibMAF8ts7TvAyUibd1TPhJmZfialJtv6tu2SEg\/640?wx_fmt=png&from=appmsg'\" data-type=\"png\" data-w=\"750\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/span><\/a><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\"><br \/><\/section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;text-align: left;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-end;padding-right: 2px;padding-left: 2px;\">\n<section style=\"margin: 10px 0% 2px;transform: translate3d(2px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 16px;color: rgb(58, 66, 94);line-height: 1;letter-spacing: 5px;text-align: center;\">\n<p><strong>\u5f80\u671f\u56de\u987e<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"height: 3px;background-color: rgb(95, 156, 239);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: 2px;margin-right: 0%;margin-left: 0%;text-align: center;line-height: 0.8;font-size: 9px;\" powered-by=\"xiumi.us\">\n<section style=\"width: 0px;display: inline-block;border-top: 0.8em solid rgb(58, 66, 94);border-left: 0.7em solid transparent !important;border-right: 0.7em solid transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;flex: 100 100 0%;align-self: flex-end;height: auto;\">\n<section style=\"margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"height: 3px;background-color: rgb(95, 156, 239);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: 2px;margin-right: 0%;margin-left: 0%;text-align: center;line-height: 0.8;font-size: 9px;\" powered-by=\"xiumi.us\">\n<section style=\"width: 0px;display: inline-block;border-top: 0.8em solid rgba(255, 255, 255, 0);border-left: 0.7em solid transparent !important;border-right: 0.7em solid transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"min-height: 40px;margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"width: 100%;margin-right: auto;margin-bottom: -10px;margin-left: auto;\">\n<table width=\"100%\">\n<tbody>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:0\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:0.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;box-shadow: rgb(0, 0, 0) 0px 0px 0px;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>01<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247497780&idx=2&sn=7eee90af6f387e4153bba13028e545a1&chksm=cf7f68f1f808e1e7dfb3a0597ef442e5c6e700ecf6ee5a035c766a4c7486c12eabd0ab218ea6&scene=21#wechat_redirect\" textvalue=\"1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff0c\u4e24\u4eba\u6210\u56e2\uff0c1\u4eba\u514d\u5355\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff0c\u4e24\u4eba\u6210\u56e2\uff0c1\u4eba\u514d\u5355\uff01<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:1\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:1.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>02<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247498878&idx=1&sn=fc34ed3f1d15d060bbaf8166adcebd58&chksm=cf7f6cbbf808e5ad0b5417e50ed75cfbd1b3a83ac43b97f1dfa718c822878a9a004990075133&scene=21#wechat_redirect\" textvalue=\"\u5355\u4e2a\u6570\u636e\u5e93\u7528\u817b\u4e86\uff1f\u591a\u6570\u636e\u5e93\u201c\u7ec4\u5408\u62f3\u201d\u5e26\u4f60\u6253\u5f00\u514d\u75ab\u6d78\u6da6\u65b0\u601d\u8def\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u5355\u4e2a\u6570\u636e\u5e93\u7528\u817b\u4e86\uff1f\u591a\u6570\u636e\u5e93\u201c\u7ec4\u5408\u62f3\u201d\u5e26\u4f60\u6253\u5f00\u514d\u75ab\u6d78\u6da6\u65b0\u601d\u8def\uff01<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:2\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:2.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>03<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247498673&idx=1&sn=1e09b74a836c1e64a62ae1f66eeb3f93&chksm=cf7f6b74f808e262de70be43fd73bf5fefdc0914e525eb2032f741a0152e619689cedc76946f&scene=21#wechat_redirect\" textvalue=\"\u5b5f\u5fb7\u5c14\u968f\u673a\u5316\u7684\u51c6\u5907\u5de5\u4f5c\uff0cGWAS\u6570\u636e\u7684\u7f51\u7ad9\u4e0b\u8f7d\u65b9\u6cd5\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"11\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u5b5f\u5fb7\u5c14\u968f\u673a\u5316\u7684\u51c6\u5907\u5de5\u4f5c\uff0cGWAS\u6570\u636e\u7684\u7f51\u7ad9\u4e0b\u8f7d\u65b9\u6cd5<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:3\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:3.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>04<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247498517&idx=1&sn=fa3c0104cee316982fe8162b79fe234f&chksm=cf7f6bd0f808e2c6cf979704bf883cfb5480444604f90da61dc286e7541fa5bb49df0a2fb58a&scene=21#wechat_redirect\" textvalue=\"\u8ddf\u7740\u5c0f\u679c\u5b66\u590d\u73b0-\u624b\u628a\u624b\u5e26\u4f60\u62ff\u4e0bIF=46.9Nature \u7ea7\u522b\u7684\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u56fe\uff01\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u8ddf\u7740\u5c0f\u679c\u5b66\u590d\u73b0-\u624b\u628a\u624b\u5e26\u4f60\u62ff\u4e0bIF=46.9Nature \u7ea7\u522b\u7684\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u56fe\uff01\uff01<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>RNA-seq\u5dee\u5f02\u5206\u6790\u5b9e\u6218\u6307\u5357\uff1a\u5229\u7528DESeq2 R\u5305\u7cbe\u51c6\u89e3\u6790\u5dee\u5f02\u8868\u8fbe\u5e76\u53ef\u89c6\u5316\u57fa\u56e0\u8c03\u63a7\uff01 \u539f\u521b &n […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/54995"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=54995"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/54995\/revisions"}],"predecessor-version":[{"id":55012,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/54995\/revisions\/55012"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=54995"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=54995"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=54995"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}