{"id":55047,"date":"2024-06-16T16:10:45","date_gmt":"2024-06-16T08:10:45","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55047"},"modified":"2024-06-16T16:10:45","modified_gmt":"2024-06-16T08:10:45","slug":"%e5%8f%aa%e9%9c%805%e5%88%86%e9%92%9f%e6%8e%8c%e6%8f%a1%e8%82%bf%e7%98%a4%e7%94%9f%e4%bf%a1%e6%96%87%e7%ab%a0%e5%bf%85%e5%a4%87%e5%88%86%e6%9e%90%e5%86%85%e5%ae%b9%ef%bc%8c%e4%ba%9a%e5%9e%8b-2","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55047","title":{"rendered":"\u53ea\u97005\u5206\u949f\u638c\u63e1\u80bf\u7624\u751f\u4fe1\u6587\u7ae0\u5fc5\u5907\u5206\u6790\u5185\u5bb9\uff0c\u4e9a\u578b\u95f4\u514d\u75ab\u68c0\u67e5\u70b9\u57fa\u56e0\u663e\u8457\u5dee\u5f02\u5206\u6790"},"content":{"rendered":"


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powered-by=\"xiumi.us\">\n<p>\u4eca\u5929\u5c0f\u82b1\u5e26\u6765\u7684\u5206\u4eab\u4e3a\u9996\u5148\u5229\u7528ConsensusCluster\u5bf9\u80bf\u7624\u6837\u672c\u8fdb\u884c\u5206\u578b\uff0c\u7136\u540e\u4e9a\u578b\u95f4\u514d\u75ab\u68c0\u67e5\u70b9\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u663e\u8457\u5dee\u5f02\u5206\u6790\uff0c\u8be5\u5206\u6790\u5728\u80bf\u7624\u9884\u540e\u76f8\u5173\u751f\u4fe1\u6587\u7ae0\u4e2d\u51fa\u73b0\u7684\u9891\u7387\u975e\u5e38\u9ad8\uff0c\u503c\u5f97\u5c0f\u4f19\u4f34\u4eec\u5b66\u4e60\u5965\uff0c\u63a5\u4e0b\u6765\u8ddf\u7740\u5c0f\u82b1\u5f00\u542f\u4eca\u5929\u7684\u5b66\u4e60\u4e4b\u65c5\u5427\uff01<br \/><\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 13px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 12px;\"><strong>\u5982\u4f55\u8fdb\u884c\u4e9a\u578b\u95f4\u514d\u75ab\u68c0\u67e5\u70b9\u57fa\u56e0\u8868\u8fbe\u663e\u8457\u5dee\u5f02\u5206\u6790\uff1f<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u5728\u8fdb\u884c\u8be5\u5206\u6790\u4e4b\u524d\uff0c\u5c0f\u82b1\u4e3a\u5c0f\u4f19\u4f34\u7b80\u5355\u4ecb\u7ecd\u4e00\u4e0b\u9700\u8981\u6ce8\u610f\u7684\u8981\u70b9\uff0c\u7b2c\u4e00\u70b9\u9996\u5148\u5bf9\u80bf\u7624\u6837\u672c\u8fdb\u884c\u5206\u578b\uff0c\u7b2c\u4e8c\u70b9\u51c6\u5907\u597d\u514d\u75ab\u68c0\u67e5\u70b9\u57fa\u56e0\uff0c\u6700\u540e\u5229\u7528ggpubr\u5305\u8fdb\u884c\u4e9a\u578b\u95f4\u514d\u75ab\u68c0\u67e5\u70b9\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u663e\u8457\u5dee\u5f02\u5206\u6790\uff0c\u7b5b\u9009\u51fa\u5728\u4e0d\u540c\u4e9a\u578b\u95f4\u6709\u663e\u8457\u5dee\u5f02\u7684\u514d\u75ab\u68c0\u67e5\u70b9\u57fa\u56e0\uff0c\u8fd9\u5c31\u662f\u5927\u6982\u7684\u5206\u6790\u6b65\u9aa4\uff0c\u4e0b\u9762\u548c\u5c0f\u82b1\u4e00\u8d77\u5f00\u59cb\u4eca\u5929\u7684\u5b9e\u64cd\u5427\uff01<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u51c6\u5907\u9700\u8981\u7684R\u5305<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5b89\u88c5\u9700\u8981\u7684R\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"tidyverse\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"ggplot2\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"ggsci\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">BiocManager::install(<span class=\"code-snippet__string\">\"ConsensusClusterPlus\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u52a0\u8f7d\u9700\u8981\u7684R\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(reshape2)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\">library(tidyverse)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggsci)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ConsensusClusterPlus)<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>ConsensusCluster\u4e00\u81f4\u6027\u805a\u7c7b\u5206\u6790<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7b5b\u9009\u51fa\u7684\u6709\u9884\u540e\u7684TEX\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u6587\u4ef6\uff0c\u884c\u540d\u4e3a\u57fa\u56e0\u540d\uff0c\u5217\u4e3a\u6837\u672c\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">expr1<- read.table(file = <span class=\"code-snippet__string\">\"expr_cluster.txt\"<\/span>,<span class=\"code-snippet__comment\">#\u5de5\u4f5c\u76ee\u5f55\u4e0b\u7684\u6587\u4ef6\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">header = <span class=\"code-snippet__built_in\">T<\/span>,<span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u6709\u5217\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">sep = <span class=\"code-snippet__string\">\"t\"<\/span>,<span class=\"code-snippet__comment\">#\u5206\u9694\u7b26<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">row.names = <span class=\"code-snippet__number\">1<\/span>,<span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u884c\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">check.names=<span class=\"code-snippet__built_in\">F<\/span>)<span class=\"code-snippet__comment\">#\u4e0d\u68c0\u67e5\u884c\u540d<\/span><\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.20462962962962963\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_GdocFCsugycOcbAYIIWGf5pvQibOvQ.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYQdlHYx8aGgKTbSmjwqdf35NN2ELy0PToJ9Cm2IG7vEzO5WGdocFCsugycOcbAYIIWGf5pvQibOvQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">dir.create(<span class=\"code-snippet__string\">'ConsensusCluster\/'<\/span>)<span class=\"code-snippet__comment\">#\u6784\u5efa\u6587\u4ef6\u5939<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#ConsensusCluster\u4e00\u81f4\u6027\u805a\u7c7b<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">results = ConsensusClusterPlus(as.matrix(expr1),<\/span><\/code><code><span class=\"code-snippet_outer\">maxK=<span class=\"code-snippet__number\">6<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">reps=<span class=\"code-snippet__number\">100<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">pItem=<span class=\"code-snippet__number\">0.8<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">pFeature=<span class=\"code-snippet__number\">1<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">title=<span class=\"code-snippet__string\">'ConsensusCluster\/'<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">clusterAlg=<span class=\"code-snippet__string\">\"km\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">distance=<span class=\"code-snippet__string\">\"euclidean\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">seed=<span class=\"code-snippet__number\">123456<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">plot=<span class=\"code-snippet__string\">\"pdf\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">icl <- calcICL(results,title = <span class=\"code-snippet__string\">'ConsensusCluster\/'<\/span>,plot = <span class=\"code-snippet__string\">'pdf'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">Kvec = <span class=\"code-snippet__number\">2<\/span>:<span class=\"code-snippet__number\">6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">x1 = <span class=\"code-snippet__number\">0.1<\/span>; x2 = <span class=\"code-snippet__number\">0.6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">PAC = <span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__literal\">NA<\/span>,<span class=\"code-snippet__built_in\">length<\/span>(Kvec))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">names<\/span>(PAC) = paste(<span class=\"code-snippet__string\">\"K=\"<\/span>,Kvec,sep=<span class=\"code-snippet__string\">\"\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(i <span class=\"code-snippet__keyword\">in<\/span> Kvec){<\/span><\/code><code><span class=\"code-snippet_outer\">M = results[[i]]$consensusMatrix<\/span><\/code><code><span class=\"code-snippet_outer\">Fn = ecdf(M[lower.tri(M)])<\/span><\/code><code><span class=\"code-snippet_outer\">PAC[i-<span class=\"code-snippet__number\">1<\/span>] = Fn(x2) - Fn(x1)<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">optK = Kvec[which.min(PAC)]<\/span><\/code><code><span class=\"code-snippet_outer\">optK<\/span><\/code><code><span class=\"code-snippet_outer\">PAC <- as.data.frame(PAC)<\/span><\/code><code><span class=\"code-snippet_outer\">PAC$K <- 2:<span class=\"code-snippet__number\">6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236PAC\u9a8c\u8bc1\u66f2\u7ebf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggplot(PAC,aes(factor(K),PAC,group=<span class=\"code-snippet__number\">1<\/span>))+<\/span><\/code><code><span class=\"code-snippet_outer\">geom_line()+<\/span><\/code><code><span class=\"code-snippet_outer\">theme_bw()+theme(panel.grid = element_blank())+<\/span><\/code><code><span class=\"code-snippet_outer\">geom_point(size=<span class=\"code-snippet__number\">4<\/span>,shape=<span class=\"code-snippet__number\">21<\/span>,color=<span class=\"code-snippet__string\">'darkred'<\/span>,fill=<span class=\"code-snippet__string\">'skyblue'<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">ylab(<span class=\"code-snippet__string\">'Proportion of ambiguous clustering'<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">xlab(<span class=\"code-snippet__string\">'Cluster number K'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">ggsave(<span class=\"code-snippet__string\">\"PAC.pdf\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6700\u5c0f\u503c\u4e5f\u5c31\u662f\u6700\u4f73K\uff0c\u4ecd\u7136\u4e3a3.<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u63d0\u53d6\u5206\u578b\u4fe1\u606f\uff0ck=3<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">clusterNum=<span class=\"code-snippet__number\">3<\/span> <span class=\"code-snippet__comment\">#k\u503c\u53d8\u5316\u8c03\u6574<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cluster=results[[clusterNum]][[<span class=\"code-snippet__string\">\"consensusClass\"<\/span>]]<\/span><\/code><code><span class=\"code-snippet_outer\">sub <- data.frame(Sample=<span class=\"code-snippet__built_in\">names<\/span>(cluster),Cluster=cluster)<\/span><\/code><code><span class=\"code-snippet_outer\">sub$Cluster <- paste0(<span class=\"code-snippet__string\">'C'<\/span>,sub$Cluster)<\/span><\/code><code><span class=\"code-snippet_outer\">table(sub$Cluster)<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(sub,file = <span class=\"code-snippet__string\">\"K3.txt\"<\/span>,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,<span class=\"code-snippet__built_in\">quote<\/span>=<span class=\"code-snippet__built_in\">F<\/span>)<span class=\"code-snippet__comment\">#\u8f93\u51fa\u7ed3\u679c<\/span><\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 15px;\"><strong>\u4e9a\u578b\u95f4\u514d\u75ab\u68c0\u67e5\u70b9\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u5dee\u5f02\u5206\u6790<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#EXP.txt\uff0c\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u6587\u4ef6\uff0c\u884c\u540d\u4e3a\u57fa\u56e0\u540d\uff0c\u5217\u540d\u4e3a\u6837\u672c\u4fe1\u606f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">exp<\/span><-read.table(<span class=\"code-snippet__string\">\"EXP.txt\"<\/span>,header=<span class=\"code-snippet__built_in\">T<\/span>,row.names=<span class=\"code-snippet__number\">1<\/span>,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,check.names=<span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.26944444444444443\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_aDpvvKSxrBVsFoNRnCictvANSz9ADQ.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYQdlHYx8aGgKTbSmjwqdf3ZMWDdmpKv80xVibiclFwibFwd1JqiaDpvvKSxrBVsFoNRnCictvANSz9ADQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u63d0\u53d6\u514d\u75ab\u68c0\u67e5\u70b9\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">df<-filter(<span class=\"code-snippet__built_in\">exp<\/span>,rownames(<span class=\"code-snippet__built_in\">exp<\/span>)%in% <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"LAG3\"<\/span>,<span class=\"code-snippet__string\">\"CD47\"<\/span>,<span class=\"code-snippet__string\">\"TIGIT\"<\/span>,<span class=\"code-snippet__string\">\"SIRPA\"<\/span>,<span class=\"code-snippet__string\">\"TNFRSF4\"<\/span>,<span class=\"code-snippet__string\">\"VTCN1\"<\/span>,<span class=\"code-snippet__string\">\"HAVCR2\"<\/span>,<span class=\"code-snippet__string\">\"ICOS\"<\/span>,<span class=\"code-snippet__string\">\"CTLA4\"<\/span>,<span class=\"code-snippet__string\">\"CD274\"<\/span>,<span class=\"code-snippet__string\">\"PDCD1\"<\/span>,<span class=\"code-snippet__string\">\"BTLA\"<\/span>,<span class=\"code-snippet__string\">\"TNFRSF9\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u884c\u5217\u8f6c\u7f6e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">df<-as.data.frame(t(df))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c06\u884c\u540d\u8f6c\u5316\u4e3a\u5217\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">df<-rownames_to_column(df,var=<span class=\"code-snippet__string\">\"Sample\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4fee\u6539\u57fa\u56e0\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">df<-rename(df,<span class=\"code-snippet__built_in\">c<\/span>(PD1=PDCD1,TIM3=HAVCR2,MYD1=SIRPA))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">##\u8fde\u63a5\u4e9a\u578b\u5206\u7ec4\u4fe1\u606f\u548c\u514d\u75ab\u68c0\u67e5\u70b9\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">immunegene<-left_join(df,sub,by=<span class=\"code-snippet__string\">\"Sample\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u957f\u5bbd\u6570\u636e\u8f6c\u5316<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">immunegene<-melt(immunegene,id.vars=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Sample\"<\/span>,<span class=\"code-snippet__string\">\"Cluster\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u5217\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">colnames(immunegene)<-<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Sample\"<\/span>,<span class=\"code-snippet__string\">\"Cluster\"<\/span>,<span class=\"code-snippet__string\">\"Gene\"<\/span>,<span class=\"code-snippet__string\">\"Expression\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236\u663e\u8457\u5dee\u5f02\u5c0f\u63d0\u7434+\u7bb1\u7ebf\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">boxplot_immunegene<- ggplot(immunegene, aes(x = Gene, y =Expression ))+<\/span><\/code><code><span class=\"code-snippet_outer\">labs(y=<span class=\"code-snippet__string\">\"Expression\"<\/span>,x= <span class=\"code-snippet__string\">\"\"<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">geom_boxplot(aes(fill = Cluster),position=position_dodge(<span class=\"code-snippet__number\">0.5<\/span>),width=<span class=\"code-snippet__number\">0.5<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">scale_fill_npg()+<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4fee\u6539\u4e3b\u9898<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">theme_classic() +<\/span><\/code><code><span class=\"code-snippet_outer\">theme(axis.title = element_text(size = <span class=\"code-snippet__number\">12<\/span>,color =<span class=\"code-snippet__string\">\"black\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.text = element_text(size= <span class=\"code-snippet__number\">12<\/span>,color = <span class=\"code-snippet__string\">\"black\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.grid.minor.y = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.grid.minor.x = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.text.x = element_text(angle = <span class=\"code-snippet__number\">45<\/span>, hjust = <span class=\"code-snippet__number\">1<\/span> ),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.grid=element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">legend.position = <span class=\"code-snippet__string\">\"top\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">legend.text = element_text(size= <span class=\"code-snippet__number\">12<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">legend.title= element_text(size= <span class=\"code-snippet__number\">12<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">) +<\/span><\/code><code><span class=\"code-snippet_outer\">stat_compare_means(aes(group = Cluster),<\/span><\/code><code><span class=\"code-snippet_outer\">label = <span class=\"code-snippet__string\">\"p.signif\"<\/span>,<span class=\"code-snippet__comment\">#\u6dfb\u52a0\u661f\u53f7\u6807\u7b7e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">method = <span class=\"code-snippet__string\">\"kruskal.test\"<\/span>,<span class=\"code-snippet__comment\">#kruskal.test\u591a\u7ec4\u68c0\u9a8c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">hide.ns = <span class=\"code-snippet__built_in\">F<\/span>)<span class=\"code-snippet__comment\">#\u663e\u793a\u4e0d\u663e\u8457\u7684<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4fdd\u5b58\u56fe\u7247<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggsave(file=<span class=\"code-snippet__string\">\"immunegene.pdf\"<\/span>,boxplot_immunegene,height=<span class=\"code-snippet__number\">10<\/span>,width=<span class=\"code-snippet__number\">15<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u7ed3\u679c\u6587\u4ef6<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"color: rgb(16, 116, 231);padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>1.immunegene.pdf<\/strong><\/p>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u8be5\u7ed3\u679c\u56fe\u7247\u4e3a\u4e9a\u578b\u95f4\u514d\u75ab\u68c0\u67e5\u70b9\u57fa\u56e0\u5dee\u5f02\u5206\u6790\u5c0f\u63d0\u7434+\u7bb1\u7ebf\u56fe\uff0c1\u8868\u793ap\u5c0f\u4e8e0.05\uff0c2\u8868\u793ap\u5c0f\u4e8e0.01\uff0c3\u8868\u793ap\u5c0f\u4e8e0.001,ns\u8868\u793a\u4e0d\u663e\u8457\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6675925925925926\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_H4mzW5Jw9WyicAlzCxL0XXia8Is7DQ.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYQdlHYx8aGgKTbSmjwqdf3dOJzJk7pUccZEkPZticU17ugvvCH4mzW5Jw9WyicAlzCxL0XXia8Is7DQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><span style=\"color: rgb(16, 116, 231);\"><strong>2.K3.txt<\/strong><\/span><\/p>\n<p>\u8be5\u7ed3\u679c\u6587\u4ef6\u4e3a\u5229\u7528ConsensusCluster\u4e00\u81f4\u6027\u805a\u7c7b\u5206\u6790\uff0cK=3\u65f6\u4e9a\u578b\u5206\u7ec4\u4fe1\u606f\u6587\u4ef6,\u7b2c\u4e00\u5217\u4e3a\u6837\u672c\u540d\uff0c\u7b2c\u4e8c\u5217\u4e3a\u5206\u7ec4\u4fe1\u606f\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.2601851851851852\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_rEqVP1CMK8sRiaPzsMBTZ4y7b9M9JQ.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYQdlHYx8aGgKTbSmjwqdf35mXIf8Iica1GibgpSbSuHRWNwKMrEqVP1CMK8sRiaPzsMBTZ4y7b9M9JQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 98%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;border-style: solid;border-width: 2px;border-color: rgb(160, 160, 160);padding: 27px;\">\n<section style=\"text-align: justify;line-height: 1.6;letter-spacing: 0px;padding-right: 6px;padding-left: 6px;\" powered-by=\"xiumi.us\">\n<p><span style=\"letter-spacing: 0px;\">\u6700\u7ec8\u5c0f\u82b1\u6210\u529f\u7684\u5b8c\u6210\u4e86\u4e9a\u578b\u95f4\u514d\u75ab\u68c0\u67e5\u70b9\u57fa\u56e0\u8868\u8fbe\u663e\u8457\u5dee\u5f02\u5206\u6790\uff0c\u770b\u8d77\u6765\u56fe\u7247\u6548\u679c\u975e\u5e38\u4e0d\u9519\uff0c\u6b22\u8fce\u5927\u5bb6\u548c\u5c0f\u82b1\u4e00\u8d77\u8ba8\u8bba\u5b66\u4e60\u5440\uff01\u514d\u75ab\u6d78\u6da6\u76f8\u5173\u5206\u6790\u5185\u5bb9\uff0c\u4f8b\u5982\u5355\u6837\u672c\u5bcc\u96c6\u7b97\u6cd5\u5206\u6790\u514d\u75ab\u6d78\u6da6\u4e30\u5ea6(http:\/\/www.biocloudservice.com\/106\/106.php)\uff0c\u8ba1\u7b9764\u79cd\u514d\u75ab\u7ec6\u80de\u76f8\u5bf9\u542b\u91cf(http:\/\/www.biocloudservice.com\/107\/107.php)\u7b49\u90fd\u53ef\u4ee5\u7528\u672c\u516c\u53f8\u65b0\u5f00\u53d1\u7684\u96f6\u4ee3\u7801\u4e91\u5e73\u53f0\u751f\u4fe1\u5206\u6790\u5c0f\u5de5\u5177\uff0c\u4e00\u952e\u5b8c\u6210\u8be5\u5206\u6790\u5965\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u6765\u5c1d\u8bd5\u5965\uff0c\u7f51\u5740\uff1ahttp:\/\/www.biocloudservice.com\/home.html\u3002\u4eca\u5929\u5c0f\u82b1\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\uff0c\u4e0b\u671f\u5728\u89c1\u5965\u3002<\/span><br \/><\/p>\n<p><br \/><\/p>\n<p>\u6b22\u8fce\u4f7f\u7528\uff1a\u4e91\u751f\u4fe1  – \u5b66\u751f\u7269\u4fe1\u606f\u5b66 (biocloudservice.com)<\/p>\n<p><br \/><\/p>\n<p>\u5982\u679c\u60f3\u7528\u670d\u52a1\u5668\u53ef\u4ee5\u8054\u7cfb\u5fae\u4fe1\uff1a18502195490\uff08\u5feb\u6765\u8054\u7cfb\u6211\u4eec\u4f7f\u7528\u5427\uff01\uff09<\/p>\n<\/section>\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row;transform: translate3d(40px, 0px, 0px);margin-bottom: -40px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 26px;height: 26px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);border-left: 1px solid rgb(160, 160, 160);border-bottom-left-radius: 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650761303&idx=2&sn=6f73471d1e82c56c0b2160d0fcd32d53&scene=21#wechat_redirect\" textvalue=\"\" linktype=\"image\" imgurl=\"https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/0?wx_fmt=jpeg\" imgdata=\"[object Object]\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.35083798882681566\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_pBN4r4MaBCk9p9v16yZwvMvS5nM7sQ-1.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"895\" style=\"\" data-recalc-dims=\"1\" \/><\/span><\/a><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3488888888888889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_wGGHoIUWPWSIgBgzuWA7zggZhslM2g.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSbGdkTKlXI3tbocicP8GUCzibJV5ibjQs6D7lQhs1pnDYZQiauBwGGHoIUWPWSIgBgzuWA7zggZhslM2g\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"900\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p powered-by=\"xiumi.us\">\uff08\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u8df3\u8f6c\uff09<\/p>\n<section style=\"text-align: left;margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;min-width: 10%;vertical-align: top;padding-right: 20px;\">\n<section style=\"transform: translate3d(20px, 0px, 0px);margin: 10px 0% -30px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);text-align: justify;font-size: 12px;\">\n<p><strong><img decoding=\"async\" loading=\"lazy\" data-ratio=\"0.866\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_DFegLFnI0KsYSJYsT76rCahl67iaoA.gif?resize=18%2C15\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_gif\/KDvR2NrGicSYQdlHYx8aGgKTbSmjwqdf3Ixf4opV1jicXVFnxIic39rAiakc1DFegLFnI0KsYSJYsT76rCahl67iaoA\/640?wx_fmt=gif'\" data-type=\"gif\" data-w=\"500\" height=\"15\" style=\"width: 18px;height: 15px;\" width=\"18\" data-recalc-dims=\"1\" \/> \u70b9\u4e00\u4e0b\u9605\u8bfb\u539f\u6587\u4e86\u89e3\u66f4\u591a\u8d44\u8baf<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 50px;height: 40px;vertical-align: top;overflow: hidden;\">\n<section style=\"text-align: center;margin-right: 0%;margin-left: 0%;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"0.7709090909090909\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_6ZzbrOxhgzOibdH5iaoMxsl2uQMpPA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYQdlHYx8aGgKTbSmjwqdf3EBNfxvjCku0HKXw1qGclYhBhy36ZzbrOxhgzOibdH5iaoMxsl2uQMpPA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"275\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u53ea\u97005\u5206\u949f\u638c\u63e1\u80bf\u7624\u751f\u4fe1\u6587\u7ae0\u5fc5\u5907\u5206\u6790\u5185\u5bb9\uff0c\u4e9a\u578b\u95f4\u514d\u75ab\u68c0\u67e5\u70b9\u57fa\u56e0\u663e\u8457\u5dee\u5f02\u5206\u6790 \u5c0f\u82b1  \u751f\u4fe1\u679c& […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55047"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=55047"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55047\/revisions"}],"predecessor-version":[{"id":55056,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55047\/revisions\/55056"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=55047"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=55047"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=55047"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}