<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 60px}.rich_media_area_extra{padding:0 60px 21px 60px}}@media (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner,body{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media{padding:20px;overflow:hidden;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}@media screen and (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media_content{overflow:hidden;color:#333;font-size:17px;word-wrap:break-word;-webkit-hyphens:auto;-ms-hyphens:auto;hyphens:auto;text-align:justify;position:relative;z-index:0}.rich_media_content *{max-width:100%!important;box-sizing:border-box!important;-webkit-box-sizing:border-box!important;word-wrap:break-word!important}.rich_media_content p{clear:both;min-height:1em}.rich_media_content em{font-style:italic}.rich_media_content fieldset{min-width:0}.rich_media_content .list-paddingleft-1,.rich_media_content .list-paddingleft-2,.rich_media_content .list-paddingleft-3{padding-left:2.2em}.rich_media_content .list-paddingleft-1 .list-paddingleft-2,.rich_media_content .list-paddingleft-2 .list-paddingleft-2,.rich_media_content .list-paddingleft-3 .list-paddingleft-2{padding-left:30px}.rich_media_content .list-paddingleft-1{padding-left:1.2em}.rich_media_content .list-paddingleft-3{padding-left:3.2em}.rich_media_content .code-snippet,.rich_media_content .code-snippet__fix{max-width:1000%!important}.rich_media_content .code-snippet *,.rich_media_content .code-snippet__fix *{max-width:1000%!important}.rich_media_title{font-size:22px;line-height:1.4;margin-bottom:13px;padding-bottom:13px;border-bottom:1px solid #e7e7eb;}@supports(-webkit-overflow-scrolling:touch){.rich_media_title{font-weight:700}}.rich_media_meta{display:inline-block;vertical-align:middle;padding:0 0 10px 0;font-size:15px;-webkit-tap-highlight-color:rgba(0,0,0,0)}.rich_media_meta.icon_appmsg_tag{margin-right:0px}.rich_media_meta.meta_tag_text{margin-right:0}.rich_media_meta_list em{font-style:normal}.rich_media_meta_text{color:#a5a5a5;}p{margin:0;}.msgBox{margin-top:20px;padding-top:20px;padding-left:50px;overflow:hidden;border-top:2px dashed #09a2ff;}.msg{padding-top:7px;clear:both;}.msgBody{float:right;width:100%;margin-left:55px;padding-bottom:15px;border-bottom:1px dashed #e0e0e0;}.userHeadImg{float:left;margin-left:-50px;}.userHeadImg img{width:40px;height:40px;margin-right:10px;border-radius:3px;}.userName{color:#888888;line-height:24px;font-size:14px;margin:5px 0 5px 0;height:24px;}.replyBody,.autherBody{color:#565656;font-size:15px;}.replyIcon{border-left:4px solid #33ab01;margin-right:5px;}.ad{text-decoration:none;color:#d6d4d4;font-size:12px;}.msgBodyReply{padding-top:5px;}.userName span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation Mono\",Menlo,Courier,monospace}.code-snippet__comment,.code-snippet__quote{color:#afafaf;font-style:italic}.code-snippet__keyword,.code-snippet__selector-tag,.code-snippet__subst{color:#ca7d37}.code-snippet__number,.code-snippet__literal,.code-snippet__variable,.code-snippet__template-variable,.code-snippet__tag .code-snippet__attr{color:#0e9ce5}.code-snippet__string,.code-snippet__doctag{color:#d14}.code-snippet__title,.code-snippet__section,.code-snippet__selector-id{color:#d14}.code-snippet__subst{font-weight:normal}.code-snippet__type,.code-snippet__class .code-snippet__title{color:#0e9ce5}.code-snippet__tag,.code-snippet__name,.code-snippet__attribute{color:#0e9ce5;font-weight:normal}.code-snippet__regexp,.code-snippet__link{color:#ca7d37}.code-snippet__symbol,.code-snippet__bullet{color:#d14}.code-snippet__built_in,.code-snippet__builtin-name{color:#ca7d37}.code-snippet__meta{color:#afafaf}.code-snippet__deletion{background:#fdd}.code-snippet__addition{background:#dfd}.code-snippet__emphasis{font-style:italic}.code-snippet__strong{font-weight:bold}.account_avatar{width:40px;height:40px;padding:0;}.account_info{display:-webkit-box;display:-webkit-flex;display:flex;-webkit-box-align:center;-webkit-align-items:center;padding:20px 0;align-items:center}.flex_bd{padding-left:14px;}.account_nickname{display:inline-block;vertical-align:middle;line-height:1.2;color:#576b95;font-size:14px}.account_desc{overflow:hidden;text-overflow:ellipsis;display:-webkit-box;-webkit-box-orient:vertical;-webkit-line-clamp:1;color:rgba(0,0,0,0.3);font-size:14px;line-height:1.2;padding-top:.4em}.msg_source_url{text-align:left;word-break:break-all;margin-top:20px;}.msg_source_url a{padding-right:10px;}.msg_source_url .url_text{color:#a8a8a8;}.video-desc{font-size:14px;margin-top:15px;color:#6c6c6c;}.msg_source_url{text-align:left;}.original_primary_card_tips{color:rgba(0,0,0,0.3);line-height:1.4;font-size:15px;}.weui-flex__item{margin-bottom:20px;padding:20px 16px;margin-top:16px;line-height:1.4;align-items:center;background-color:#f7f7f7;border-radius:8px;position:relative;}.original_primary_desc{color:rgba(0,0,0,0.5);font-size:14px;padding-top:4px;width:auto;overflow:hidden;text-overflow:ellipsis;}.msgBodyReplyList{border-top:1px solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"\u8d85\u8be6\u7ec6\u7684\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u70ed\u56fe\u53ef\u89c6\u5316\u4ee3\u7801\u89e3\u6790\",\"time\":\"2023-08-31 19:00:59\",\"timeStamp\":\"1693479659\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491713&idx=3&sn=1975e896fa1efceba124185e4aa57c57&chksm=cf7f7044f808f952ce95cdbd187f5853485fd6d62c70f7bb25a1399708e552b0922ba6d6c0d6#rd\" target=\"_blank\" rel=\"noopener\">\u8d85\u8be6\u7ec6\u7684\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u70ed\u56fe\u53ef\u89c6\u5316\u4ee3\u7801\u89e3\u6790<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u82b1 <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-08-31 19:00:59<\/em><\/div>\n<p><content><\/p>\n<div class=\"reprint\">\n<p>\u8f6c\u81ea\u516c\u4f17\u53f7\uff1a\u751f\u4fe1\u82b1<br \/><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzkzODUxNjcwMA==&mid=2247484365&idx=1&sn=6eb9535e54dacf5356c384e10c6d277d\" target=\"_blank\" title=\"\u9605\u8bfb\u539f\u6587\" rel=\"noopener\">http:\/\/mp.weixin.qq.com\/s?__biz=MzkzODUxNjcwMA==&mid=2247484365&idx=1&sn=6eb9535e54dacf5356c384e10c6d277d<\/a><\/p>\n<\/div>\n<section style=\"font-size: 14px;margin-bottom: 24px;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(65, 128, 216);\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;padding-left: 12px;\">\n<section style=\"text-align: justify;color: rgb(255, 255, 255);\" powered-by=\"xiumi.us\">\n<p><strong>\u70b9\u51fb\u84dd\u5b57 \u5173\u6ce8\u6211\u4eec<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;margin-right: -17px;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;margin-right: -17px;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(201, 229, 70);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><span style=\"text-indent: 2em;\">\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u7684\u70ed\u56fe<\/span><span style=\"text-indent: 2em;\">\u662f\u901a\u8fc7\u53ef\u89c6\u5316\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u8868\u8fbe\u6a21\u5f0f\u6765\u63ed\u793a\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u4e2d\u7684\u91cd\u8981\u53d8\u5316\u3002\u7ed8\u5236\u5dee\u5f02\u57fa\u56e0\u7684\u70ed\u56fe\u3002\u70ed\u56fe\u53ef\u4ee5\u5e2e\u52a9\u53d1\u73b0\u5728\u4e0d\u540c\u6761\u4ef6\u4e0b\u663e\u8457\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u3002\u901a\u8fc7\u8bbe\u7f6e\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u7684\u9608\u503c\uff0c\u4f8b\u5982logFC\uff08fold change\uff09\u548cp\u503c\u6216FDR\uff08false discovery rate\uff09\uff0c\u7b5b\u9009\u51fa\u663e\u8457\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\uff0c\u5e76\u5728\u70ed\u56fe\u4e2d\u7a81\u51fa\u663e\u793a\u8fd9\u4e9b\u57fa\u56e0\u3002\u8fd9\u6709\u52a9\u4e8e\u5feb\u901f\u9274\u5b9a\u4e0e\u7279\u5b9a\u751f\u7269\u5b66\u6761\u4ef6\u6216\u75be\u75c5\u72b6\u6001\u76f8\u5173\u7684\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u3002<\/span><\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u4e0b\u9762\u5c0f\u82b1\u5e26\u4f60\u7528vcan\u57fa\u56e0\u8fdb\u884c\u64cd\u4f5c\uff0c\u6570\u636e\u4f7f\u7528tcga\u4e0b\u8f7d\u7684\u4f60\u8981\u7814\u7a76\u7684\u764c\u79cd\u6570\u636e\u77e9\u9635\u5c31\u53ef\u4ee5\u3002<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#if (!requireNamespace(\"BiocManager\", quietly = TRUE))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># install.packages(\"BiocManager\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#BiocManager::install(\"limma\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#install.packages(\"pheatmap\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">\u5b89\u88c5limma\u548cpheatmap\u3002\u9996\u5148\u68c0\u67e5\u662f\u5426\u5df2\u7ecf\u5b89\u88c5\u4e86BiocManager\u5305\uff0c<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5f15\u7528\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(limma)<\/span><\/code><code><span class=\"code-snippet_outer\">library(pheatmap)<\/span><\/code><code><span class=\"code-snippet_outer\">\u52a0\u8f7d\u6240\u9700\u7684R\u5305\uff0c\u4ee5\u4fbf\u5728\u4ee3\u7801\u4e2d\u4f7f\u7528\u76f8\u5e94\u7684\u51fd\u6570\u548c\u529f\u80fd\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">gene=<span class=\"code-snippet__string\">\"VCAN\"<\/span> <span class=\"code-snippet__comment\">#\u793a\u4f8b\u76ee\u6807\u57fa\u56e0\u7684\u540d\u79f0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">expFile=<span class=\"code-snippet__string\">\"symbol.txt\"<\/span> <span class=\"code-snippet__comment\">#\u8868\u8fbe\u8f93\u5165\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">logFCfilter=<span class=\"code-snippet__number\">1<\/span> <span class=\"code-snippet__comment\">#logFC\u8fc7\u6ee4\u6761\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">fdrFilter=<span class=\"code-snippet__number\">0.05<\/span> <span class=\"code-snippet__comment\">#fdr\u8fc7\u6ee4\u6761\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">setwd(<span class=\"code-snippet__string\">\"C:\\biowolf\\Gene\\19.diff\"<\/span>) <span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u5de5\u4f5c\u76ee\u5f55<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">\u6307\u5b9a\u76ee\u6807\u57fa\u56e0\u7684\u540d\u79f0\u3001\u8868\u8fbe\u8f93\u5165\u6587\u4ef6\u7684\u540d\u79f0\u3001logFC\u8fc7\u6ee4\u6761\u4ef6\u548cfdr\u8fc7\u6ee4\u6761\u4ef6\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">\u53e6\u5916\uff0c\u901a\u8fc7setwd\u51fd\u6570\u8bbe\u7f6eR\u7684\u5de5\u4f5c\u76ee\u5f55<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6\u6587\u4ef6,\u5e76\u5bf9\u8f93\u5165\u6587\u4ef6\u6574\u7406<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">rt=read.table(expFile, header=<span class=\"code-snippet__built_in\">T<\/span>, sep=<span class=\"code-snippet__string\">\"t\"<\/span>, check.names=<span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">\u539f\u59cb\u57fa\u56e0\u8868\u8fbe\u91cf\u6570\u636esymbol.xt<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.0932539682539681\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_pc59rayjm8GPficazbGqMFKG8jVsAg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZSHVb7KTxFibicpz22gq6ic3kujEXzAfBDzswNZjIuOyBpGgUgpc59rayjm8GPficazbGqMFKG8jVsAg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"504\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">rt=as.matrix(rt)<\/span><\/code><code><span class=\"code-snippet_outer\">rownames(rt)=rt[,<span class=\"code-snippet__number\">1<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">exp<\/span>=rt[,<span class=\"code-snippet__number\">2<\/span>:ncol(rt)]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">dimnames<\/span>=<span class=\"code-snippet__built_in\">list<\/span>(rownames(<span class=\"code-snippet__built_in\">exp<\/span>), colnames(<span class=\"code-snippet__built_in\">exp<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\">data=matrix(<span class=\"code-snippet__built_in\">as.numeric<\/span>(as.matrix(<span class=\"code-snippet__built_in\">exp<\/span>)),nrow=nrow(<span class=\"code-snippet__built_in\">exp<\/span>), <span class=\"code-snippet__built_in\">dimnames<\/span>=<span class=\"code-snippet__built_in\">dimnames<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">data=avereps(data)<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9040139616055847\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_NCtYzP3XOmXuZkKhevyicpEwXibwZQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZSHVb7KTxFibicpz22gq6ic3klMMVLH9NOKwNNzKHFGVevhKicIENCtYzP3XOmXuZkKhevyicpEwXibwZQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"573\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8f93\u5165\u6587\u4ef6\u4e2d\u8bfb\u53d6\u8868\u8fbe\u6570\u636e\uff0c\u5e76\u5bf9\u6570\u636e\u8fdb\u884c\u6574\u7406\u3002\u8868\u8fbe\u6570\u636e\u662f\u57fa\u56e0\u7684\u8868\u8fbe\u91cf\u6570\u636e\uff0c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6\u540e\u8f6c\u6362\u4e3a\u77e9\u9635\u5f62\u5f0f\uff0c\u5e76\u8bbe\u7f6e\u884c\u540d\u548c\u5217\u540d\u3002\u4f7f\u7528avereps\u51fd\u6570\u5bf9\u6570\u636e\u8fdb\u884c\u5e73<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5747\u5904\u7406\uff0c\u5904\u7406\u540e\u7684\u6570\u636e\u5b58\u50a8\u5728data\u53d8\u91cf\u4e2d\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u53bb\u9664\u6b63\u5e38\u6837\u54c1<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(colnames(data),<span class=\"code-snippet__string\">\"\\-\"<\/span>), <span class=\"code-snippet__string\">\"[\"<\/span>, <span class=\"code-snippet__number\">4<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(group,<span class=\"code-snippet__string\">\"\"<\/span>), <span class=\"code-snippet__string\">\"[\"<\/span>, <span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">group=gsub(<span class=\"code-snippet__string\">\"2\"<\/span>, <span class=\"code-snippet__string\">\"1\"<\/span>, group)<\/span><\/code><code><span class=\"code-snippet_outer\">data=data[,group==<span class=\"code-snippet__number\">0<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">data=t(data)<\/span><\/code><code><span class=\"code-snippet_outer\">rownames(data)=gsub(<span class=\"code-snippet__string\">\"(.*?)\\-(.*?)\\-(.*?)\\-(.*?)\\-.*\"<\/span>,<span class=\"code-snippet__string\">\"\\1\\-\\2\\-\\3\"<\/span>, rownames(data))<\/span><\/code><code><span class=\"code-snippet_outer\">rt=t(avereps(data))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6839\u636e\u6837\u54c1\u540d\u79f0\u4e2d\u7684\u7f16\u53f7\u4fe1\u606f\uff0c\u5c06\u6b63\u5e38\u6837\u54c1\u548c\u5176\u4ed6\u6837\u54c1\u5206\u5f00\u3002\u901a\u8fc7\u6b63\u5219\u8868\u8fbe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5f0f\u64cd\u4f5c\uff0c\u5c06\u6837\u54c1\u540d\u4e2d\u7684\u90e8\u5206\u4fe1\u606f\u63d0\u53d6\u51fa\u6765\uff0c\u5e76\u53bb\u9664\u6837\u54c1\u7f16\u53f7\u4e2d\u7684\"2\"\u3002\u7136\u540e\uff0c#\u6839\u636e\u6837\u54c1\u540d\u4e2d\u7684\u4fe1\u606f\u91cd\u65b0\u547d\u540d\u884c\u540d\uff0c\u5e76\u5c06\u6570\u636e\u91cd\u65b0\u8f6c\u7f6e\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6309\u7167\u57fa\u56e0\u8868\u8fbe\u7684\u4e2d\u4f4d\u503c\u5bf9\u6837\u54c1\u5206\u7ec4<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">low=rt[gene,]<=median(rt[gene,])<\/span><\/code><code><span class=\"code-snippet_outer\">high=rt[gene,]>median(rt[gene,])<\/span><\/code><code><span class=\"code-snippet_outer\">lowRT=rt[,low]<\/span><\/code><code><span class=\"code-snippet_outer\">highRT=rt[,high]<\/span><\/code><code><span class=\"code-snippet_outer\">conNum=ncol(lowRT) <span class=\"code-snippet__comment\">#\u4f4e\u8868\u8fbe\u7ec4\u6837\u54c1\u6570\u76ee<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">treatNum=ncol(highRT) <span class=\"code-snippet__comment\">#\u9ad8\u8868\u8fbe\u7ec4\u6837\u54c1\u6570\u76ee<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data=cbind(lowRT,highRT)<\/span><\/code><code><span class=\"code-snippet_outer\">Type=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__number\">1<\/span>,conNum), <span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__number\">2<\/span>,treatNum))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6839\u636e\u76ee\u6807\u57fa\u56e0\u7684\u8868\u8fbe\u503c\u4e2d\u4f4d\u6570\uff0c\u5c06\u6837\u54c1\u5206\u4e3a\u4f4e\u8868\u8fbe\u7ec4\u548c\u9ad8\u8868\u8fbe\u7ec4\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6839\u636e\u4f4e\u8868\u8fbe\u7ec4\u548c\u9ad8\u8868\u8fbe\u7ec4\u7684\u5217\u6570\uff0c\u5206\u522b\u5b58\u50a8\u5728lowRT\u548chighRT\u53d8\u91cf\u4e2d\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__number\">3<\/span>conNum\u548ctreatNum\u5206\u522b\u8868\u793a\u4f4e\u8868\u8fbe\u7ec4\u548c\u9ad8\u8868\u8fbe\u7ec4\u7684\u6837\u54c1\u6570\u76ee\u3002<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4e24\u4e2a\u7ec4\u7684\u6570\u636e\u5408\u5e76\u4e3a\u4e00\u4e2a\u65b0\u7684\u6570\u636e\u77e9\u9635\uff0c\u7528Type\u5411\u91cf\u8868\u793a\u6bcf\u4e2a\u6837\u54c1\u6240\u5c5e\u7ec4\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5dee\u5f02\u5206\u6790<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">outTab=data.frame()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(i <span class=\"code-snippet__keyword\">in<\/span> row.names(data)){<\/span><\/code><code><span class=\"code-snippet_outer\">rt=data.frame(<span class=\"code-snippet__built_in\">expression<\/span>=data[i,], Type=Type)<\/span><\/code><code><span class=\"code-snippet_outer\">wilcoxTest=wilcox.test(<span class=\"code-snippet__built_in\">expression<\/span> ~ Type, data=rt)<\/span><\/code><code><span class=\"code-snippet_outer\">conGeneMeans=mean(data[i,<span class=\"code-snippet__number\">1<\/span>:conNum])<\/span><\/code><code><span class=\"code-snippet_outer\">treatGeneMeans=mean(data[i,(conNum+<span class=\"code-snippet__number\">1<\/span>):ncol(data)])<\/span><\/code><code><span class=\"code-snippet_outer\">logFC=log2(treatGeneMeans)-log2(conGeneMeans)<\/span><\/code><code><span class=\"code-snippet_outer\">pvalue=wilcoxTest$p.value<\/span><\/code><code><span class=\"code-snippet_outer\">conMed=median(data[i,<span class=\"code-snippet__number\">1<\/span>:conNum])<\/span><\/code><code><span class=\"code-snippet_outer\">treatMed=median(data[i,(conNum+<span class=\"code-snippet__number\">1<\/span>):ncol(data)])<\/span><\/code><code><span class=\"code-snippet_outer\">diffMed=treatMed-conMed<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span>( ((logFC><span class=\"code-snippet__number\">0<\/span>) & (diffMed><span class=\"code-snippet__number\">0<\/span>)) | ((logFC<<span class=\"code-snippet__number\">0<\/span>) & (diffMed<<span class=\"code-snippet__number\">0<\/span>)) ){<\/span><\/code><code><span class=\"code-snippet_outer\">outTab=rbind(outTab,cbind(gene=i,lowMean=conGeneMeans,highMean=treatGeneMeans,logFC=logFC,pValue=pvalue))<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">pValue=outTab[,<span class=\"code-snippet__string\">\"pValue\"<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">fdr=p.adjust(<span class=\"code-snippet__built_in\">as.numeric<\/span>(as.vector(pValue)), method=<span class=\"code-snippet__string\">\"fdr\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">outTab=cbind(outTab, fdr=fdr)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5bf9\u4e8e\u6bcf\u4e2a\u57fa\u56e0\uff0c\u901a\u8fc7Wilcoxon\u79e9\u548c\u68c0\u9a8c\u6bd4\u8f83\u4f4e\u8868\u8fbe\u7ec4\u548c\u9ad8\u8868\u8fbe\u7ec4\u4e4b\u95f4\u7684\u5dee\u5f02\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8ba1\u7b97\u4f4e\u8868\u8fbe\u7ec4\u548c\u9ad8\u8868\u8fbe\u7ec4\u7684\u57fa\u56e0\u5747\u503c\u3001logFC\u3001p\u503c\u548c\u5dee\u5f02\u4e2d\u503c\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6839\u636elogFC\u7684\u65b9\u5411\u548c\u5dee\u5f02\u4e2d\u503c\u7684\u65b9\u5411\uff0c\u5c06\u7b26\u5408\u6761\u4ef6\u7684\u57fa\u56e0\u7ed3\u679c\u6dfb\u52a0\u5230outTab\u6570\u636e\u6846\u4e2d\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#pValue\u662fp\u503c\u7684\u5411\u91cf\uff0c\u4f7f\u7528p.adjust\u51fd\u6570\u8ba1\u7b97\u591a\u91cd\u68c0\u9a8c\u6821\u6b63\u540e\u7684fdr\u503c\uff0c\u5c06fdr<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u503c\u6dfb\u52a0\u5230outTab\u4e2d\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8f93\u51fa\u5dee\u5f02\u7684\u7ed3\u679c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">outDiff=outTab[( <span class=\"code-snippet__built_in\">abs<\/span>(<span class=\"code-snippet__built_in\">as.numeric<\/span>(as.vector(outTab$logFC)))>logFCfilter & <span class=\"code-snippet__built_in\">as.numeric<\/span>(as.vector(outTab$fdr))<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(outDiff, file=<span class=\"code-snippet__string\">\"diff.txt\"<\/span>, sep=<span class=\"code-snippet__string\">\"t\"<\/span>, row.names=<span class=\"code-snippet__built_in\">F<\/span>, <span class=\"code-snippet__built_in\">quote<\/span>=<span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8f93\u51fa\u7b26\u5408\u8fc7\u6ee4\u6761\u4ef6\u7684\u5dee\u5f02\u57fa\u56e0\u7ed3\u679c\u3002\u6839\u636e\u8bbe\u5b9a\u7684logFC\u8fc7\u6ee4\u6761\u4ef6\u548cfdr\u8fc7\u6ee4\u6761\u4ef6\uff0c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7b5b\u9009\u51fa\u5dee\u5f02\u57fa\u56e0\uff0c\u5e76\u5c06\u7ed3\u679c\u5199\u5165\u5230\u540d\u4e3a\"diff.txt\"\u7684\u6587\u4ef6\u4e2d\u3002<\/span><\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5067264573991032\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_qWLfzJ0m2iaoJsa8v8C4bdQkVB7gfA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZSHVb7KTxFibicpz22gq6ic3k5RVES0h1xfmrJ1BuEdkoNyNAOqWLfzJ0m2iaoJsa8v8C4bdQkVB7gfA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"446\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.8106904231625836\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_Nw7agPia43dCkF661UGJXfsJvEH3KA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZSHVb7KTxFibicpz22gq6ic3kDicvrkp6chtUAkIdzJ70meFjsPNw7agPia43dCkF661UGJXfsJvEH3KA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"449\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236\u5dee\u5f02\u57fa\u56e0\u7684\u70ed\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">geneNum=<span class=\"code-snippet__number\">50<\/span> <span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u663e\u793a\u57fa\u56e0\u7684\u6570\u76ee<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">outDiff=outDiff[order(<span class=\"code-snippet__built_in\">as.numeric<\/span>(as.vector(outDiff$logFC))),]<\/span><\/code><code><span class=\"code-snippet_outer\">diffGeneName=as.vector(outDiff[,<span class=\"code-snippet__number\">1<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\">diffLength=<span class=\"code-snippet__built_in\">length<\/span>(diffGeneName)<\/span><\/code><code><span class=\"code-snippet_outer\">hmGene=<span class=\"code-snippet__built_in\">c<\/span>()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span>(diffLength>(<span class=\"code-snippet__number\">2<\/span>*geneNum)){<\/span><\/code><code><span class=\"code-snippet_outer\">hmGene=diffGeneName[<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__number\">1<\/span>:geneNum,(diffLength-geneNum+<span class=\"code-snippet__number\">1<\/span>):diffLength)]<\/span><\/code><code><span class=\"code-snippet_outer\">}<span class=\"code-snippet__keyword\">else<\/span>{<\/span><\/code><code><span class=\"code-snippet_outer\">hmGene=diffGeneName<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">hmExp=log2(data[hmGene,]+<span class=\"code-snippet__number\">0.01<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">Type=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__string\">\"Low\"<\/span>,conNum),<span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__string\">\"High\"<\/span>,treatNum))<\/span><\/code><code><span class=\"code-snippet_outer\">Type=factor(Type, levels=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Low\"<\/span>,<span class=\"code-snippet__string\">\"High\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">names<\/span>(Type)=colnames(data)<\/span><\/code><code><span class=\"code-snippet_outer\">Type=as.data.frame(Type)<\/span><\/code><code><span class=\"code-snippet_outer\">pdf(file=<span class=\"code-snippet__string\">\"heatmap.pdf\"<\/span>, width=<span class=\"code-snippet__number\">10<\/span>, height=<span class=\"code-snippet__number\">7<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236\u5dee\u5f02\u57fa\u56e0\u7684\u70ed\u56fe\u3002\u9996\u5148\uff0c\u6839\u636e\u8bbe\u5b9a\u7684\u57fa\u56e0\u6570\u76ee\uff0c\u9009\u62e9\u8981\u663e\u793a\u7684\u5dee\u5f02\u57fa\u56e0\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u79f0\u3002\u6839\u636e\u9009\u62e9\u7684\u5dee\u5f02\u57fa\u56e0\uff0c\u4ece\u539f\u59cb\u6570\u636e\u4e2d\u63d0\u53d6\u76f8\u5e94\u7684\u8868\u8fbe\u503c\uff0c\u5e76\u8fdb\u884clog2\u8f6c\u6362\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#Type\u5411\u91cf\u8868\u793a\u6bcf\u4e2a\u6837\u54c1\u6240\u5c5e\u7684\u7ec4\uff0c\u6839\u636e\u6837\u54c1\u7684\u5206\u7ec4\u60c5\u51b5\u521b\u5efa\u4e00\u4e2a\u6570\u636e\u6846\u3002\u63a5\u4e0b#\u6765\uff0c\u4f7f\u7528pheatmap\u51fd\u6570\u7ed8\u5236\u70ed\u56fe\u3002\u8bbe\u7f6e\u70ed\u56fe\u7684\u53c2\u6570\uff0c\u5305\u62ec\u6ce8\u91ca\u3001\u989c\u8272\u6e10\u53d8\u3001<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u662f\u5426\u8fdb\u884c\u5217\u805a\u7c7b\u3001\u662f\u5426\u663e\u793a\u5217\u540d\u3001\u884c\u7f29\u653e\u7b49\u3002\u5c06\u7ed8\u5236\u7684\u70ed\u56fe\u4fdd\u5b58\u4e3aPDF\u6587\u4ef6\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pheatmap(hmExp,<\/span><\/code><code><span class=\"code-snippet_outer\">annotation=Type,<\/span><\/code><code><span class=\"code-snippet_outer\">color = colorRampPalette(<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__string\">\"blue\"<\/span>,<span class=\"code-snippet__number\">5<\/span>), <span class=\"code-snippet__string\">\"white\"<\/span>, <span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__string\">\"red\"<\/span>,<span class=\"code-snippet__number\">5<\/span>)))(<span class=\"code-snippet__number\">50<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">cluster_cols =<span class=\"code-snippet__built_in\">F<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">show_colnames = <span class=\"code-snippet__built_in\">F<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">scale=<span class=\"code-snippet__string\">\"row\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">fontsize = <span class=\"code-snippet__number\">8<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">fontsize_row=<span class=\"code-snippet__number\">5<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">fontsize_col=<span class=\"code-snippet__number\">8<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">dev.off()<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7183098591549296\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_b9lKicP0NMcsp6mUWEbXQHPaRdbqZg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZSHVb7KTxFibicpz22gq6ic3kc7ichoapviaQec6CuEkY8St9xPYb9lKicP0NMcsp6mUWEbXQHPaRdbqZg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"923\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 98%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;border-style: solid;border-width: 2px;border-color: rgb(160, 160, 160);padding: 27px;\">\n<section style=\"text-align: justify;line-height: 1.6;letter-spacing: 0px;padding-right: 6px;padding-left: 6px;\" powered-by=\"xiumi.us\">\n<p>\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u7684\u70ed\u56fe\u5177\u6709\u53ef\u89c6\u5316\u5dee\u5f02\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\u3001\u53d1\u73b0\u5dee\u5f02\u57fa\u56e0\u3001\u7814\u7a76\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u548c\u751f\u7269\u8fc7\u7a0b\u3001\u6307\u5bfc\u751f\u7269\u5b66\u7814\u7a76\u548c\u5b9e\u9a8c\u8bbe\u8ba1\uff0c\u4ee5\u53ca\u53ef\u89c6\u5316\u548c\u62a5\u544a\u7ed3\u679c\u7684\u529f\u80fd\u548c\u610f\u4e49\u3002\u5b83\u5728\u751f\u7269\u4fe1\u606f\u5b66\u548c\u751f\u7269\u533b\u5b66\u7814\u7a76\u4e2d\u8d77\u7740\u91cd\u8981\u7684\u4f5c\u7528\u3002\u4e0b\u671f\u5c06\u4e3a\u4f60\u5e26\u6765\u66f4\u591aR\u8bed\u8a00\u7684\u9a9a\u64cd\u4f5c\u6280\u5de7\uff0c\u4ee5\u4e0b\u63a8\u8350\u7684\u662f\u4e00\u4e2a\u591a\u529f\u80fd\u7684\u751f\u4fe1\u5e73\u53f0\u3002<\/p>\n<p><br \/><\/p>\n<p>\u6b22\u8fce\u4f7f\u7528\uff1a\u4e91\u751f\u4fe1 – \u5b66\u751f\u7269\u4fe1\u606f\u5b66 (biocloudservice.com)<\/p>\n<p><br \/><\/p>\n<p>\u5982\u679c\u60f3\u7528\u670d\u52a1\u5668\u53ef\u4ee5\u8054\u7cfb\u5fae\u4fe1\uff1a18502195490\uff08\u5feb\u6765\u8054\u7cfb\u6211\u4eec\u4f7f\u7528\u5427\uff01\uff09<\/p>\n<\/section>\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row;transform: translate3d(40px, 0px, 0px);margin-bottom: -40px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 26px;height: 26px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);border-left: 1px solid rgb(160, 160, 160);border-bottom-left-radius: 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650761303&idx=2&sn=6f73471d1e82c56c0b2160d0fcd32d53&scene=21#wechat_redirect\" textvalue=\"\" linktype=\"image\" imgurl=\"https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/0?wx_fmt=jpeg\" imgdata=\"[object Object]\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.35083798882681566\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_pBN4r4MaBCk9p9v16yZwvMvS5nM7sQ-10.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"895\" style=\"\" data-recalc-dims=\"1\" \/><\/span><\/a><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3488888888888889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_uKaqczIP6EMQhkT9sW7c1bKv6z9AAg-10.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdD9CpdQZl2iaySUTmNepgg2BpMuKaqczIP6EMQhkT9sW7c1bKv6z9AAg\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"900\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p powered-by=\"xiumi.us\">\uff08\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u8df3\u8f6c\uff09<\/p>\n<section style=\"text-align: left;margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;min-width: 10%;vertical-align: top;padding-right: 20px;\">\n<section style=\"transform: translate3d(20px, 0px, 0px);margin: 10px 0% -30px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);text-align: justify;font-size: 12px;\">\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.866\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_ZKKNC0rY7Kz65X0m2X5eMmGtUPCbXA.gif?resize=18%2C15\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_gif\/KDvR2NrGicSZSHVb7KTxFibicpz22gq6ic3k10YqgWKrosdgl2FjuQlPThdzZKKNC0rY7Kz65X0m2X5eMmGtUPCbXA\/640?wx_fmt=gif'\" data-type=\"gif\" data-w=\"500\" height=\"15\" style=\"width: 18px;height: 15px;\" width=\"18\" data-recalc-dims=\"1\" \/> \u70b9\u4e00\u4e0b\u9605\u8bfb\u539f\u6587\u4e86\u89e3\u66f4\u591a\u8d44\u8baf<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 50px;height: 40px;vertical-align: top;overflow: hidden;\">\n<section style=\"text-align: center;margin-right: 0%;margin-left: 0%;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7709090909090909\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_cJjqyjCz2lzia8Pq7Eg0icJloF7xlg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZSHVb7KTxFibicpz22gq6ic3k3ibdd2NFhhmRiaSEibnR5mm0311CwcJjqyjCz2lzia8Pq7Eg0icJloF7xlg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"275\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u8d85\u8be6\u7ec6\u7684\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u70ed\u56fe\u53ef\u89c6\u5316\u4ee3\u7801\u89e3\u6790 \u5c0f\u82b1 \u751f\u4fe1\u679c 2023-0 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55057"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=55057"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55057\/revisions"}],"predecessor-version":[{"id":55067,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55057\/revisions\/55067"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=55057"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=55057"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=55057"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

{"id":55057,"date":"2024-06-16T16:11:16","date_gmt":"2024-06-16T08:11:16","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55057"},"modified":"2024-06-16T16:11:16","modified_gmt":"2024-06-16T08:11:16","slug":"%e8%b6%85%e8%af%a6%e7%bb%86%e7%9a%84%e5%b7%ae%e5%bc%82%e8%a1%a8%e8%be%be%e5%88%86%e6%9e%90%e7%83%ad%e5%9b%be%e5%8f%af%e8%a7%86%e5%8c%96%e4%bb%a3%e7%a0%81%e8%a7%a3%e6%9e%90","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55057","title":{"rendered":"\u8d85\u8be6\u7ec6\u7684\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u70ed\u56fe\u53ef\u89c6\u5316\u4ee3\u7801\u89e3\u6790"},"content":{"rendered":"

\n
\n<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 60px}.rich_media_area_extra{padding:0 60px 21px 60px}}@media (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner,body{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media{padding:20px;overflow:hidden;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}@media screen and (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media_content{overflow:hidden;color:#333;font-size:17px;word-wrap:break-word;-webkit-hyphens:auto;-ms-hyphens:auto;hyphens:auto;text-align:justify;position:relative;z-index:0}.rich_media_content *{max-width:100%!important;box-sizing:border-box!important;-webkit-box-sizing:border-box!important;word-wrap:break-word!important}.rich_media_content p{clear:both;min-height:1em}.rich_media_content em{font-style:italic}.rich_media_content fieldset{min-width:0}.rich_media_content .list-paddingleft-1,.rich_media_content .list-paddingleft-2,.rich_media_content .list-paddingleft-3{padding-left:2.2em}.rich_media_content .list-paddingleft-1 .list-paddingleft-2,.rich_media_content .list-paddingleft-2 .list-paddingleft-2,.rich_media_content .list-paddingleft-3 .list-paddingleft-2{padding-left:30px}.rich_media_content .list-paddingleft-1{padding-left:1.2em}.rich_media_content .list-paddingleft-3{padding-left:3.2em}.rich_media_content .code-snippet,.rich_media_content .code-snippet__fix{max-width:1000%!important}.rich_media_content .code-snippet *,.rich_media_content .code-snippet__fix *{max-width:1000%!important}.rich_media_title{font-size:22px;line-height:1.4;margin-bottom:13px;padding-bottom:13px;border-bottom:1px solid #e7e7eb;}@supports(-webkit-overflow-scrolling:touch){.rich_media_title{font-weight:700}}.rich_media_meta{display:inline-block;vertical-align:middle;padding:0 0 10px 0;font-size:15px;-webkit-tap-highlight-color:rgba(0,0,0,0)}.rich_media_meta.icon_appmsg_tag{margin-right:0px}.rich_media_meta.meta_tag_text{margin-right:0}.rich_media_meta_list em{font-style:normal}.rich_media_meta_text{color:#a5a5a5;}p{margin:0;}.msgBox{margin-top:20px;padding-top:20px;padding-left:50px;overflow:hidden;border-top:2px dashed #09a2ff;}.msg{padding-top:7px;clear:both;}.msgBody{float:right;width:100%;margin-left:55px;padding-bottom:15px;border-bottom:1px dashed #e0e0e0;}.userHeadImg{float:left;margin-left:-50px;}.userHeadImg img{width:40px;height:40px;margin-right:10px;border-radius:3px;}.userName{color:#888888;line-height:24px;font-size:14px;margin:5px 0 5px 0;height:24px;}.replyBody,.autherBody{color:#565656;font-size:15px;}.replyIcon{border-left:4px solid #33ab01;margin-right:5px;}.ad{text-decoration:none;color:#d6d4d4;font-size:12px;}.msgBodyReply{padding-top:5px;}.userName span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation Mono\",Menlo,Courier,monospace}.code-snippet__comment,.code-snippet__quote{color:#afafaf;font-style:italic}.code-snippet__keyword,.code-snippet__selector-tag,.code-snippet__subst{color:#ca7d37}.code-snippet__number,.code-snippet__literal,.code-snippet__variable,.code-snippet__template-variable,.code-snippet__tag .code-snippet__attr{color:#0e9ce5}.code-snippet__string,.code-snippet__doctag{color:#d14}.code-snippet__title,.code-snippet__section,.code-snippet__selector-id{color:#d14}.code-snippet__subst{font-weight:normal}.code-snippet__type,.code-snippet__class .code-snippet__title{color:#0e9ce5}.code-snippet__tag,.code-snippet__name,.code-snippet__attribute{color:#0e9ce5;font-weight:normal}.code-snippet__regexp,.code-snippet__link{color:#ca7d37}.code-snippet__symbol,.code-snippet__bullet{color:#d14}.code-snippet__built_in,.code-snippet__builtin-name{color:#ca7d37}.code-snippet__meta{color:#afafaf}.code-snippet__deletion{background:#fdd}.code-snippet__addition{background:#dfd}.code-snippet__emphasis{font-style:italic}.code-snippet__strong{font-weight:bold}.account_avatar{width:40px;height:40px;padding:0;}.account_info{display:-webkit-box;display:-webkit-flex;display:flex;-webkit-box-align:center;-webkit-align-items:center;padding:20px 0;align-items:center}.flex_bd{padding-left:14px;}.account_nickname{display:inline-block;vertical-align:middle;line-height:1.2;color:#576b95;font-size:14px}.account_desc{overflow:hidden;text-overflow:ellipsis;display:-webkit-box;-webkit-box-orient:vertical;-webkit-line-clamp:1;color:rgba(0,0,0,0.3);font-size:14px;line-height:1.2;padding-top:.4em}.msg_source_url{text-align:left;word-break:break-all;margin-top:20px;}.msg_source_url a{padding-right:10px;}.msg_source_url .url_text{color:#a8a8a8;}.video-desc{font-size:14px;margin-top:15px;color:#6c6c6c;}.msg_source_url{text-align:left;}.original_primary_card_tips{color:rgba(0,0,0,0.3);line-height:1.4;font-size:15px;}.weui-flex__item{margin-bottom:20px;padding:20px 16px;margin-top:16px;line-height:1.4;align-items:center;background-color:#f7f7f7;border-radius:8px;position:relative;}.original_primary_desc{color:rgba(0,0,0,0.5);font-size:14px;padding-top:4px;width:auto;overflow:hidden;text-overflow:ellipsis;}.msgBodyReplyList{border-top:1px solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"\u8d85\u8be6\u7ec6\u7684\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u70ed\u56fe\u53ef\u89c6\u5316\u4ee3\u7801\u89e3\u6790\",\"time\":\"2023-08-31 19:00:59\",\"timeStamp\":\"1693479659\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491713&idx=3&sn=1975e896fa1efceba124185e4aa57c57&chksm=cf7f7044f808f952ce95cdbd187f5853485fd6d62c70f7bb25a1399708e552b0922ba6d6c0d6#rd\" target=\"_blank\" rel=\"noopener\">\u8d85\u8be6\u7ec6\u7684\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u70ed\u56fe\u53ef\u89c6\u5316\u4ee3\u7801\u89e3\u6790<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u82b1 <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-08-31 19:00:59<\/em><\/div>\n<p><content><\/p>\n<div class=\"reprint\">\n<p>\u8f6c\u81ea\u516c\u4f17\u53f7\uff1a\u751f\u4fe1\u82b1<br \/><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzkzODUxNjcwMA==&mid=2247484365&idx=1&sn=6eb9535e54dacf5356c384e10c6d277d\" target=\"_blank\" title=\"\u9605\u8bfb\u539f\u6587\" rel=\"noopener\">http:\/\/mp.weixin.qq.com\/s?__biz=MzkzODUxNjcwMA==&mid=2247484365&idx=1&sn=6eb9535e54dacf5356c384e10c6d277d<\/a><\/p>\n<\/div>\n<section style=\"font-size: 14px;margin-bottom: 24px;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(65, 128, 216);\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;padding-left: 12px;\">\n<section style=\"text-align: justify;color: rgb(255, 255, 255);\" powered-by=\"xiumi.us\">\n<p><strong>\u70b9\u51fb\u84dd\u5b57 \u5173\u6ce8\u6211\u4eec<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;margin-right: -17px;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;margin-right: -17px;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(201, 229, 70);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><span style=\"text-indent: 2em;\">\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u7684\u70ed\u56fe<\/span><span style=\"text-indent: 2em;\">\u662f\u901a\u8fc7\u53ef\u89c6\u5316\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u8868\u8fbe\u6a21\u5f0f\u6765\u63ed\u793a\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u4e2d\u7684\u91cd\u8981\u53d8\u5316\u3002\u7ed8\u5236\u5dee\u5f02\u57fa\u56e0\u7684\u70ed\u56fe\u3002\u70ed\u56fe\u53ef\u4ee5\u5e2e\u52a9\u53d1\u73b0\u5728\u4e0d\u540c\u6761\u4ef6\u4e0b\u663e\u8457\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u3002\u901a\u8fc7\u8bbe\u7f6e\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u7684\u9608\u503c\uff0c\u4f8b\u5982logFC\uff08fold change\uff09\u548cp\u503c\u6216FDR\uff08false discovery rate\uff09\uff0c\u7b5b\u9009\u51fa\u663e\u8457\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\uff0c\u5e76\u5728\u70ed\u56fe\u4e2d\u7a81\u51fa\u663e\u793a\u8fd9\u4e9b\u57fa\u56e0\u3002\u8fd9\u6709\u52a9\u4e8e\u5feb\u901f\u9274\u5b9a\u4e0e\u7279\u5b9a\u751f\u7269\u5b66\u6761\u4ef6\u6216\u75be\u75c5\u72b6\u6001\u76f8\u5173\u7684\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u3002<\/span><\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u4e0b\u9762\u5c0f\u82b1\u5e26\u4f60\u7528vcan\u57fa\u56e0\u8fdb\u884c\u64cd\u4f5c\uff0c\u6570\u636e\u4f7f\u7528tcga\u4e0b\u8f7d\u7684\u4f60\u8981\u7814\u7a76\u7684\u764c\u79cd\u6570\u636e\u77e9\u9635\u5c31\u53ef\u4ee5\u3002<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#if (!requireNamespace(\"BiocManager\", quietly = TRUE))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># install.packages(\"BiocManager\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#BiocManager::install(\"limma\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#install.packages(\"pheatmap\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">\u5b89\u88c5limma\u548cpheatmap\u3002\u9996\u5148\u68c0\u67e5\u662f\u5426\u5df2\u7ecf\u5b89\u88c5\u4e86BiocManager\u5305\uff0c<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5f15\u7528\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(limma)<\/span><\/code><code><span class=\"code-snippet_outer\">library(pheatmap)<\/span><\/code><code><span class=\"code-snippet_outer\">\u52a0\u8f7d\u6240\u9700\u7684R\u5305\uff0c\u4ee5\u4fbf\u5728\u4ee3\u7801\u4e2d\u4f7f\u7528\u76f8\u5e94\u7684\u51fd\u6570\u548c\u529f\u80fd\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">gene=<span class=\"code-snippet__string\">\"VCAN\"<\/span> <span class=\"code-snippet__comment\">#\u793a\u4f8b\u76ee\u6807\u57fa\u56e0\u7684\u540d\u79f0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">expFile=<span class=\"code-snippet__string\">\"symbol.txt\"<\/span> <span class=\"code-snippet__comment\">#\u8868\u8fbe\u8f93\u5165\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">logFCfilter=<span class=\"code-snippet__number\">1<\/span> <span class=\"code-snippet__comment\">#logFC\u8fc7\u6ee4\u6761\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">fdrFilter=<span class=\"code-snippet__number\">0.05<\/span> <span class=\"code-snippet__comment\">#fdr\u8fc7\u6ee4\u6761\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">setwd(<span class=\"code-snippet__string\">\"C:\\biowolf\\Gene\\19.diff\"<\/span>) <span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u5de5\u4f5c\u76ee\u5f55<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">\u6307\u5b9a\u76ee\u6807\u57fa\u56e0\u7684\u540d\u79f0\u3001\u8868\u8fbe\u8f93\u5165\u6587\u4ef6\u7684\u540d\u79f0\u3001logFC\u8fc7\u6ee4\u6761\u4ef6\u548cfdr\u8fc7\u6ee4\u6761\u4ef6\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">\u53e6\u5916\uff0c\u901a\u8fc7setwd\u51fd\u6570\u8bbe\u7f6eR\u7684\u5de5\u4f5c\u76ee\u5f55<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6\u6587\u4ef6,\u5e76\u5bf9\u8f93\u5165\u6587\u4ef6\u6574\u7406<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">rt=read.table(expFile, header=<span class=\"code-snippet__built_in\">T<\/span>, sep=<span class=\"code-snippet__string\">\"t\"<\/span>, check.names=<span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">\u539f\u59cb\u57fa\u56e0\u8868\u8fbe\u91cf\u6570\u636esymbol.xt<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.0932539682539681\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_pc59rayjm8GPficazbGqMFKG8jVsAg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZSHVb7KTxFibicpz22gq6ic3kujEXzAfBDzswNZjIuOyBpGgUgpc59rayjm8GPficazbGqMFKG8jVsAg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"504\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">rt=as.matrix(rt)<\/span><\/code><code><span class=\"code-snippet_outer\">rownames(rt)=rt[,<span class=\"code-snippet__number\">1<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">exp<\/span>=rt[,<span class=\"code-snippet__number\">2<\/span>:ncol(rt)]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">dimnames<\/span>=<span class=\"code-snippet__built_in\">list<\/span>(rownames(<span class=\"code-snippet__built_in\">exp<\/span>), colnames(<span class=\"code-snippet__built_in\">exp<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\">data=matrix(<span class=\"code-snippet__built_in\">as.numeric<\/span>(as.matrix(<span class=\"code-snippet__built_in\">exp<\/span>)),nrow=nrow(<span class=\"code-snippet__built_in\">exp<\/span>), <span class=\"code-snippet__built_in\">dimnames<\/span>=<span class=\"code-snippet__built_in\">dimnames<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">data=avereps(data)<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9040139616055847\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_NCtYzP3XOmXuZkKhevyicpEwXibwZQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZSHVb7KTxFibicpz22gq6ic3klMMVLH9NOKwNNzKHFGVevhKicIENCtYzP3XOmXuZkKhevyicpEwXibwZQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"573\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8f93\u5165\u6587\u4ef6\u4e2d\u8bfb\u53d6\u8868\u8fbe\u6570\u636e\uff0c\u5e76\u5bf9\u6570\u636e\u8fdb\u884c\u6574\u7406\u3002\u8868\u8fbe\u6570\u636e\u662f\u57fa\u56e0\u7684\u8868\u8fbe\u91cf\u6570\u636e\uff0c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6\u540e\u8f6c\u6362\u4e3a\u77e9\u9635\u5f62\u5f0f\uff0c\u5e76\u8bbe\u7f6e\u884c\u540d\u548c\u5217\u540d\u3002\u4f7f\u7528avereps\u51fd\u6570\u5bf9\u6570\u636e\u8fdb\u884c\u5e73<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5747\u5904\u7406\uff0c\u5904\u7406\u540e\u7684\u6570\u636e\u5b58\u50a8\u5728data\u53d8\u91cf\u4e2d\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u53bb\u9664\u6b63\u5e38\u6837\u54c1<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(colnames(data),<span class=\"code-snippet__string\">\"\\-\"<\/span>), <span class=\"code-snippet__string\">\"[\"<\/span>, <span class=\"code-snippet__number\">4<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(group,<span class=\"code-snippet__string\">\"\"<\/span>), <span class=\"code-snippet__string\">\"[\"<\/span>, <span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">group=gsub(<span class=\"code-snippet__string\">\"2\"<\/span>, <span class=\"code-snippet__string\">\"1\"<\/span>, group)<\/span><\/code><code><span class=\"code-snippet_outer\">data=data[,group==<span class=\"code-snippet__number\">0<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">data=t(data)<\/span><\/code><code><span class=\"code-snippet_outer\">rownames(data)=gsub(<span class=\"code-snippet__string\">\"(.*?)\\-(.*?)\\-(.*?)\\-(.*?)\\-.*\"<\/span>,<span class=\"code-snippet__string\">\"\\1\\-\\2\\-\\3\"<\/span>, rownames(data))<\/span><\/code><code><span class=\"code-snippet_outer\">rt=t(avereps(data))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6839\u636e\u6837\u54c1\u540d\u79f0\u4e2d\u7684\u7f16\u53f7\u4fe1\u606f\uff0c\u5c06\u6b63\u5e38\u6837\u54c1\u548c\u5176\u4ed6\u6837\u54c1\u5206\u5f00\u3002\u901a\u8fc7\u6b63\u5219\u8868\u8fbe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5f0f\u64cd\u4f5c\uff0c\u5c06\u6837\u54c1\u540d\u4e2d\u7684\u90e8\u5206\u4fe1\u606f\u63d0\u53d6\u51fa\u6765\uff0c\u5e76\u53bb\u9664\u6837\u54c1\u7f16\u53f7\u4e2d\u7684\"2\"\u3002\u7136\u540e\uff0c#\u6839\u636e\u6837\u54c1\u540d\u4e2d\u7684\u4fe1\u606f\u91cd\u65b0\u547d\u540d\u884c\u540d\uff0c\u5e76\u5c06\u6570\u636e\u91cd\u65b0\u8f6c\u7f6e\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6309\u7167\u57fa\u56e0\u8868\u8fbe\u7684\u4e2d\u4f4d\u503c\u5bf9\u6837\u54c1\u5206\u7ec4<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">low=rt[gene,]<=median(rt[gene,])<\/span><\/code><code><span class=\"code-snippet_outer\">high=rt[gene,]>median(rt[gene,])<\/span><\/code><code><span class=\"code-snippet_outer\">lowRT=rt[,low]<\/span><\/code><code><span class=\"code-snippet_outer\">highRT=rt[,high]<\/span><\/code><code><span class=\"code-snippet_outer\">conNum=ncol(lowRT) <span class=\"code-snippet__comment\">#\u4f4e\u8868\u8fbe\u7ec4\u6837\u54c1\u6570\u76ee<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">treatNum=ncol(highRT) <span class=\"code-snippet__comment\">#\u9ad8\u8868\u8fbe\u7ec4\u6837\u54c1\u6570\u76ee<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data=cbind(lowRT,highRT)<\/span><\/code><code><span class=\"code-snippet_outer\">Type=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__number\">1<\/span>,conNum), <span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__number\">2<\/span>,treatNum))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6839\u636e\u76ee\u6807\u57fa\u56e0\u7684\u8868\u8fbe\u503c\u4e2d\u4f4d\u6570\uff0c\u5c06\u6837\u54c1\u5206\u4e3a\u4f4e\u8868\u8fbe\u7ec4\u548c\u9ad8\u8868\u8fbe\u7ec4\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6839\u636e\u4f4e\u8868\u8fbe\u7ec4\u548c\u9ad8\u8868\u8fbe\u7ec4\u7684\u5217\u6570\uff0c\u5206\u522b\u5b58\u50a8\u5728lowRT\u548chighRT\u53d8\u91cf\u4e2d\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__number\">3<\/span>conNum\u548ctreatNum\u5206\u522b\u8868\u793a\u4f4e\u8868\u8fbe\u7ec4\u548c\u9ad8\u8868\u8fbe\u7ec4\u7684\u6837\u54c1\u6570\u76ee\u3002<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4e24\u4e2a\u7ec4\u7684\u6570\u636e\u5408\u5e76\u4e3a\u4e00\u4e2a\u65b0\u7684\u6570\u636e\u77e9\u9635\uff0c\u7528Type\u5411\u91cf\u8868\u793a\u6bcf\u4e2a\u6837\u54c1\u6240\u5c5e\u7ec4\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5dee\u5f02\u5206\u6790<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">outTab=data.frame()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(i <span class=\"code-snippet__keyword\">in<\/span> row.names(data)){<\/span><\/code><code><span class=\"code-snippet_outer\">rt=data.frame(<span class=\"code-snippet__built_in\">expression<\/span>=data[i,], Type=Type)<\/span><\/code><code><span class=\"code-snippet_outer\">wilcoxTest=wilcox.test(<span class=\"code-snippet__built_in\">expression<\/span> ~ Type, data=rt)<\/span><\/code><code><span class=\"code-snippet_outer\">conGeneMeans=mean(data[i,<span class=\"code-snippet__number\">1<\/span>:conNum])<\/span><\/code><code><span class=\"code-snippet_outer\">treatGeneMeans=mean(data[i,(conNum+<span class=\"code-snippet__number\">1<\/span>):ncol(data)])<\/span><\/code><code><span class=\"code-snippet_outer\">logFC=log2(treatGeneMeans)-log2(conGeneMeans)<\/span><\/code><code><span class=\"code-snippet_outer\">pvalue=wilcoxTest$p.value<\/span><\/code><code><span class=\"code-snippet_outer\">conMed=median(data[i,<span class=\"code-snippet__number\">1<\/span>:conNum])<\/span><\/code><code><span class=\"code-snippet_outer\">treatMed=median(data[i,(conNum+<span class=\"code-snippet__number\">1<\/span>):ncol(data)])<\/span><\/code><code><span class=\"code-snippet_outer\">diffMed=treatMed-conMed<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span>( ((logFC><span class=\"code-snippet__number\">0<\/span>) & (diffMed><span class=\"code-snippet__number\">0<\/span>)) | ((logFC<<span class=\"code-snippet__number\">0<\/span>) & (diffMed<<span class=\"code-snippet__number\">0<\/span>)) ){<\/span><\/code><code><span class=\"code-snippet_outer\">outTab=rbind(outTab,cbind(gene=i,lowMean=conGeneMeans,highMean=treatGeneMeans,logFC=logFC,pValue=pvalue))<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">pValue=outTab[,<span class=\"code-snippet__string\">\"pValue\"<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">fdr=p.adjust(<span class=\"code-snippet__built_in\">as.numeric<\/span>(as.vector(pValue)), method=<span class=\"code-snippet__string\">\"fdr\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">outTab=cbind(outTab, fdr=fdr)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5bf9\u4e8e\u6bcf\u4e2a\u57fa\u56e0\uff0c\u901a\u8fc7Wilcoxon\u79e9\u548c\u68c0\u9a8c\u6bd4\u8f83\u4f4e\u8868\u8fbe\u7ec4\u548c\u9ad8\u8868\u8fbe\u7ec4\u4e4b\u95f4\u7684\u5dee\u5f02\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8ba1\u7b97\u4f4e\u8868\u8fbe\u7ec4\u548c\u9ad8\u8868\u8fbe\u7ec4\u7684\u57fa\u56e0\u5747\u503c\u3001logFC\u3001p\u503c\u548c\u5dee\u5f02\u4e2d\u503c\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6839\u636elogFC\u7684\u65b9\u5411\u548c\u5dee\u5f02\u4e2d\u503c\u7684\u65b9\u5411\uff0c\u5c06\u7b26\u5408\u6761\u4ef6\u7684\u57fa\u56e0\u7ed3\u679c\u6dfb\u52a0\u5230outTab\u6570\u636e\u6846\u4e2d\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#pValue\u662fp\u503c\u7684\u5411\u91cf\uff0c\u4f7f\u7528p.adjust\u51fd\u6570\u8ba1\u7b97\u591a\u91cd\u68c0\u9a8c\u6821\u6b63\u540e\u7684fdr\u503c\uff0c\u5c06fdr<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u503c\u6dfb\u52a0\u5230outTab\u4e2d\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8f93\u51fa\u5dee\u5f02\u7684\u7ed3\u679c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">outDiff=outTab[( <span class=\"code-snippet__built_in\">abs<\/span>(<span class=\"code-snippet__built_in\">as.numeric<\/span>(as.vector(outTab$logFC)))>logFCfilter & <span class=\"code-snippet__built_in\">as.numeric<\/span>(as.vector(outTab$fdr))<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(outDiff, file=<span class=\"code-snippet__string\">\"diff.txt\"<\/span>, sep=<span class=\"code-snippet__string\">\"t\"<\/span>, row.names=<span class=\"code-snippet__built_in\">F<\/span>, <span class=\"code-snippet__built_in\">quote<\/span>=<span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8f93\u51fa\u7b26\u5408\u8fc7\u6ee4\u6761\u4ef6\u7684\u5dee\u5f02\u57fa\u56e0\u7ed3\u679c\u3002\u6839\u636e\u8bbe\u5b9a\u7684logFC\u8fc7\u6ee4\u6761\u4ef6\u548cfdr\u8fc7\u6ee4\u6761\u4ef6\uff0c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7b5b\u9009\u51fa\u5dee\u5f02\u57fa\u56e0\uff0c\u5e76\u5c06\u7ed3\u679c\u5199\u5165\u5230\u540d\u4e3a\"diff.txt\"\u7684\u6587\u4ef6\u4e2d\u3002<\/span><\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5067264573991032\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_qWLfzJ0m2iaoJsa8v8C4bdQkVB7gfA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZSHVb7KTxFibicpz22gq6ic3k5RVES0h1xfmrJ1BuEdkoNyNAOqWLfzJ0m2iaoJsa8v8C4bdQkVB7gfA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"446\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.8106904231625836\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_Nw7agPia43dCkF661UGJXfsJvEH3KA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZSHVb7KTxFibicpz22gq6ic3kDicvrkp6chtUAkIdzJ70meFjsPNw7agPia43dCkF661UGJXfsJvEH3KA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"449\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236\u5dee\u5f02\u57fa\u56e0\u7684\u70ed\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">geneNum=<span class=\"code-snippet__number\">50<\/span> <span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u663e\u793a\u57fa\u56e0\u7684\u6570\u76ee<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">outDiff=outDiff[order(<span class=\"code-snippet__built_in\">as.numeric<\/span>(as.vector(outDiff$logFC))),]<\/span><\/code><code><span class=\"code-snippet_outer\">diffGeneName=as.vector(outDiff[,<span class=\"code-snippet__number\">1<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\">diffLength=<span class=\"code-snippet__built_in\">length<\/span>(diffGeneName)<\/span><\/code><code><span class=\"code-snippet_outer\">hmGene=<span class=\"code-snippet__built_in\">c<\/span>()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span>(diffLength>(<span class=\"code-snippet__number\">2<\/span>*geneNum)){<\/span><\/code><code><span class=\"code-snippet_outer\">hmGene=diffGeneName[<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__number\">1<\/span>:geneNum,(diffLength-geneNum+<span class=\"code-snippet__number\">1<\/span>):diffLength)]<\/span><\/code><code><span class=\"code-snippet_outer\">}<span class=\"code-snippet__keyword\">else<\/span>{<\/span><\/code><code><span class=\"code-snippet_outer\">hmGene=diffGeneName<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">hmExp=log2(data[hmGene,]+<span class=\"code-snippet__number\">0.01<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">Type=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__string\">\"Low\"<\/span>,conNum),<span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__string\">\"High\"<\/span>,treatNum))<\/span><\/code><code><span class=\"code-snippet_outer\">Type=factor(Type, levels=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Low\"<\/span>,<span class=\"code-snippet__string\">\"High\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">names<\/span>(Type)=colnames(data)<\/span><\/code><code><span class=\"code-snippet_outer\">Type=as.data.frame(Type)<\/span><\/code><code><span class=\"code-snippet_outer\">pdf(file=<span class=\"code-snippet__string\">\"heatmap.pdf\"<\/span>, width=<span class=\"code-snippet__number\">10<\/span>, height=<span class=\"code-snippet__number\">7<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236\u5dee\u5f02\u57fa\u56e0\u7684\u70ed\u56fe\u3002\u9996\u5148\uff0c\u6839\u636e\u8bbe\u5b9a\u7684\u57fa\u56e0\u6570\u76ee\uff0c\u9009\u62e9\u8981\u663e\u793a\u7684\u5dee\u5f02\u57fa\u56e0\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u79f0\u3002\u6839\u636e\u9009\u62e9\u7684\u5dee\u5f02\u57fa\u56e0\uff0c\u4ece\u539f\u59cb\u6570\u636e\u4e2d\u63d0\u53d6\u76f8\u5e94\u7684\u8868\u8fbe\u503c\uff0c\u5e76\u8fdb\u884clog2\u8f6c\u6362\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#Type\u5411\u91cf\u8868\u793a\u6bcf\u4e2a\u6837\u54c1\u6240\u5c5e\u7684\u7ec4\uff0c\u6839\u636e\u6837\u54c1\u7684\u5206\u7ec4\u60c5\u51b5\u521b\u5efa\u4e00\u4e2a\u6570\u636e\u6846\u3002\u63a5\u4e0b#\u6765\uff0c\u4f7f\u7528pheatmap\u51fd\u6570\u7ed8\u5236\u70ed\u56fe\u3002\u8bbe\u7f6e\u70ed\u56fe\u7684\u53c2\u6570\uff0c\u5305\u62ec\u6ce8\u91ca\u3001\u989c\u8272\u6e10\u53d8\u3001<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u662f\u5426\u8fdb\u884c\u5217\u805a\u7c7b\u3001\u662f\u5426\u663e\u793a\u5217\u540d\u3001\u884c\u7f29\u653e\u7b49\u3002\u5c06\u7ed8\u5236\u7684\u70ed\u56fe\u4fdd\u5b58\u4e3aPDF\u6587\u4ef6\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pheatmap(hmExp,<\/span><\/code><code><span class=\"code-snippet_outer\">annotation=Type,<\/span><\/code><code><span class=\"code-snippet_outer\">color = colorRampPalette(<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__string\">\"blue\"<\/span>,<span class=\"code-snippet__number\">5<\/span>), <span class=\"code-snippet__string\">\"white\"<\/span>, <span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__string\">\"red\"<\/span>,<span class=\"code-snippet__number\">5<\/span>)))(<span class=\"code-snippet__number\">50<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">cluster_cols =<span class=\"code-snippet__built_in\">F<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">show_colnames = <span class=\"code-snippet__built_in\">F<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">scale=<span class=\"code-snippet__string\">\"row\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">fontsize = <span class=\"code-snippet__number\">8<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">fontsize_row=<span class=\"code-snippet__number\">5<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">fontsize_col=<span class=\"code-snippet__number\">8<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">dev.off()<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7183098591549296\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_b9lKicP0NMcsp6mUWEbXQHPaRdbqZg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZSHVb7KTxFibicpz22gq6ic3kc7ichoapviaQec6CuEkY8St9xPYb9lKicP0NMcsp6mUWEbXQHPaRdbqZg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"923\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 98%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;border-style: solid;border-width: 2px;border-color: rgb(160, 160, 160);padding: 27px;\">\n<section style=\"text-align: justify;line-height: 1.6;letter-spacing: 0px;padding-right: 6px;padding-left: 6px;\" powered-by=\"xiumi.us\">\n<p>\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u7684\u70ed\u56fe\u5177\u6709\u53ef\u89c6\u5316\u5dee\u5f02\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\u3001\u53d1\u73b0\u5dee\u5f02\u57fa\u56e0\u3001\u7814\u7a76\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u548c\u751f\u7269\u8fc7\u7a0b\u3001\u6307\u5bfc\u751f\u7269\u5b66\u7814\u7a76\u548c\u5b9e\u9a8c\u8bbe\u8ba1\uff0c\u4ee5\u53ca\u53ef\u89c6\u5316\u548c\u62a5\u544a\u7ed3\u679c\u7684\u529f\u80fd\u548c\u610f\u4e49\u3002\u5b83\u5728\u751f\u7269\u4fe1\u606f\u5b66\u548c\u751f\u7269\u533b\u5b66\u7814\u7a76\u4e2d\u8d77\u7740\u91cd\u8981\u7684\u4f5c\u7528\u3002\u4e0b\u671f\u5c06\u4e3a\u4f60\u5e26\u6765\u66f4\u591aR\u8bed\u8a00\u7684\u9a9a\u64cd\u4f5c\u6280\u5de7\uff0c\u4ee5\u4e0b\u63a8\u8350\u7684\u662f\u4e00\u4e2a\u591a\u529f\u80fd\u7684\u751f\u4fe1\u5e73\u53f0\u3002<\/p>\n<p><br \/><\/p>\n<p>\u6b22\u8fce\u4f7f\u7528\uff1a\u4e91\u751f\u4fe1 – \u5b66\u751f\u7269\u4fe1\u606f\u5b66 (biocloudservice.com)<\/p>\n<p><br \/><\/p>\n<p>\u5982\u679c\u60f3\u7528\u670d\u52a1\u5668\u53ef\u4ee5\u8054\u7cfb\u5fae\u4fe1\uff1a18502195490\uff08\u5feb\u6765\u8054\u7cfb\u6211\u4eec\u4f7f\u7528\u5427\uff01\uff09<\/p>\n<\/section>\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row;transform: translate3d(40px, 0px, 0px);margin-bottom: -40px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 26px;height: 26px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);border-left: 1px solid rgb(160, 160, 160);border-bottom-left-radius: 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650761303&idx=2&sn=6f73471d1e82c56c0b2160d0fcd32d53&scene=21#wechat_redirect\" textvalue=\"\" linktype=\"image\" imgurl=\"https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/0?wx_fmt=jpeg\" imgdata=\"[object Object]\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.35083798882681566\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_pBN4r4MaBCk9p9v16yZwvMvS5nM7sQ-10.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"895\" style=\"\" data-recalc-dims=\"1\" \/><\/span><\/a><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3488888888888889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_uKaqczIP6EMQhkT9sW7c1bKv6z9AAg-10.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdD9CpdQZl2iaySUTmNepgg2BpMuKaqczIP6EMQhkT9sW7c1bKv6z9AAg\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"900\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p powered-by=\"xiumi.us\">\uff08\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u8df3\u8f6c\uff09<\/p>\n<section style=\"text-align: left;margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;min-width: 10%;vertical-align: top;padding-right: 20px;\">\n<section style=\"transform: translate3d(20px, 0px, 0px);margin: 10px 0% -30px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);text-align: justify;font-size: 12px;\">\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.866\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_ZKKNC0rY7Kz65X0m2X5eMmGtUPCbXA.gif?resize=18%2C15\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_gif\/KDvR2NrGicSZSHVb7KTxFibicpz22gq6ic3k10YqgWKrosdgl2FjuQlPThdzZKKNC0rY7Kz65X0m2X5eMmGtUPCbXA\/640?wx_fmt=gif'\" data-type=\"gif\" data-w=\"500\" height=\"15\" style=\"width: 18px;height: 15px;\" width=\"18\" data-recalc-dims=\"1\" \/> \u70b9\u4e00\u4e0b\u9605\u8bfb\u539f\u6587\u4e86\u89e3\u66f4\u591a\u8d44\u8baf<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 50px;height: 40px;vertical-align: top;overflow: hidden;\">\n<section style=\"text-align: center;margin-right: 0%;margin-left: 0%;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7709090909090909\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_cJjqyjCz2lzia8Pq7Eg0icJloF7xlg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSZSHVb7KTxFibicpz22gq6ic3k3ibdd2NFhhmRiaSEibnR5mm0311CwcJjqyjCz2lzia8Pq7Eg0icJloF7xlg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"275\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u8d85\u8be6\u7ec6\u7684\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u70ed\u56fe\u53ef\u89c6\u5316\u4ee3\u7801\u89e3\u6790 \u5c0f\u82b1 \u751f\u4fe1\u679c 2023-0 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55057"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=55057"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55057\/revisions"}],"predecessor-version":[{"id":55067,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55057\/revisions\/55067"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=55057"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=55057"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=55057"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}