{"id":55096,"date":"2024-06-16T16:13:20","date_gmt":"2024-06-16T08:13:20","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55096"},"modified":"2024-06-16T16:13:20","modified_gmt":"2024-06-16T08:13:20","slug":"%e5%b0%8f%e6%9e%9c%e4%ba%94%e5%88%86%e9%92%9f%e6%95%99%e4%bc%9a%e4%bd%a0%e5%a6%82%e4%bd%95%e4%bd%bf%e7%94%a8r%e8%af%ad%e8%a8%80%e5%8c%85scuttle%e8%bf%9b%e8%a1%8crna%e6%95%b0%e6%8d%ae%e5%88%86%e6%9e%90","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55096","title":{"rendered":"\u5c0f\u679c\u4e94\u5206\u949f\u6559\u4f1a\u4f60\u5982\u4f55\u4f7f\u7528R\u8bed\u8a00\u5305scuttle\u8fdb\u884cRNA\u6570\u636e\u5206\u6790"},"content":{"rendered":"


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href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2821072943068839938#wechat_redirect\" title=\"\u751f\u4fe1\u7ed8\u56fe\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u7ed8\u56fe<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=3138222091851530242#wechat_redirect\" title=\"\u751f\u4fe1\u6709\u5173\u7edf\u8ba1\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u6709\u5173\u7edf\u8ba1<\/a><\/p>\n<\/div>\n<p style=\"text-align: center;\" data-mpa-powered-by=\"yiban.io\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.23333333333333334\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_rE2v0O5WiaUDJjgv5bxK2G1ePHyH2g-32.jpg?w=640\" 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sans-serif;\">\u6b63\u662f\u5728\u8fd9\u79cd\u80cc\u666f\u4e0b\uff0cscuttle\u5d2d\u9732\u5934\u89d2\u3002\u4f5c\u4e3a\u4e00\u4e2a\u4e13\u6ce8\u4e8e\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\u7684R\u5305\uff0cscuttle\u65e8\u5728\u534f\u52a9\u751f\u7269\u5b66\u5bb6\u548c\u751f\u7269\u4fe1\u606f\u5b66\u5bb6\u89e3\u6790\u8fd9\u4e9b\u590d\u6742\u7684\u6570\u636e\uff0c\u4e3a\u4ed6\u4eec\u63d0\u4f9b\u4e86\u4e00\u5957\u5f3a\u5927\u7684\u5206\u6790\u548c\u53ef\u89c6\u5316\u5de5\u5177\uff0c\u5e2e\u52a9\u6df1\u5165\u4e86\u89e3\u7ec6\u80de\u7684\u591a\u6837\u6027\u3001\u53d1\u80b2\u8fc7\u7a0b\u548c\u75be\u75c5\u673a\u5236\u3002\u901a\u8fc7scuttle\uff0c\u6211\u4eec\u80fd\u591f\u5728\u7ec6\u80de\u7684\u5fae\u89c2\u5c42\u9762\u4e0a\u6316\u6398\u51fa\u524d\u6240\u672a\u89c1\u7684\u6d1e\u5bdf\u529b\u3002<\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5728\u8fd9\u4e2a\u591a\u529f\u80fd\u7684\u5305\u4e2d\uff0cscuttle\u9996\u5148\u63d0\u4f9b\u4e86\u6570\u636e\u7684\u8d28\u91cf\u63a7\u5236\u548c\u9884\u5904\u7406\u529f\u80fd\uff0c\u5e2e\u52a9\u7528\u6237\u8fc7\u6ee4\u6389\u4f4e\u8d28\u91cf\u7684\u7ec6\u80de\u548c\u57fa\u56e0\uff0c\u540c\u65f6\u6821\u6b63\u6279\u6b21\u6548\u5e94\uff0c\u786e\u4fdd\u540e\u7eed\u5206\u6790\u7684\u53ef\u9760\u6027\u3002\u8fd9\u4e00\u6b65\u9aa4\u7684\u5173\u952e\u6027\u5728\u4e8e\u4fdd\u969c\u6570\u636e\u7684\u51c6\u786e\u6027\uff0c\u4e3a\u540e\u7eed\u7684\u63a2\u7d22\u6253\u4e0b\u575a\u5b9e\u7684\u57fa\u7840\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">         <\/span><o:p style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\"> <\/o:p><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">I. \u6df1\u5165\u4e86\u89e3\u7ec6\u80de\u591a\u6837\u6027\u4e0e\u53d1\u80b2\u8fc7\u7a0b<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">\u5728\u73b0\u4ee3\u751f\u7269\u5b66\u9886\u57df\uff0c\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6280\u672f\u7684\u5174\u8d77\u4e3a\u6211\u4eec\u6253\u5f00\u4e86\u89c2\u5bdf\u7ec6\u80de\u5185\u90e8\u5de5\u4f5c\u673a\u5236\u7684\u65b0\u89c6\u89d2\u3002scuttle\u4f5c\u4e3a\u4e00\u6b3e\u4e13\u6ce8\u4e8e\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\u5206\u6790\u7684R\u5305\uff0c\u53d1\u6325\u7740\u72ec\u7279\u7684\u4f18\u52bf\u3002\u5176\u663e\u8457\u529f\u80fd\u4e4b\u4e00\u4fbf\u662f\u901a\u8fc7\u805a\u7c7b\u548c\u964d\u7ef4\u5206\u6790\u5e2e\u52a9\u751f\u7269\u5b66\u5bb6\u6df1\u5165\u7406\u89e3\u7ec6\u80de\u7684\u591a\u6837\u6027\u548c\u53d1\u80b2\u8fc7\u7a0b\u3002\u5728\u7ec6\u80de\u7684\u8f6c\u5f55\u7ec4\u6c34\u5e73\u4e0a\uff0c\u4e0d\u540c\u7c7b\u578b\u7684\u7ec6\u80de\u53ef\u80fd\u8868\u73b0\u51fa\u5de8\u5927\u7684\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\uff0c\u8fd9\u4e9b\u5dee\u5f02\u5f80\u5f80\u76f4\u63a5\u5173\u8054\u7740\u7ec6\u80de\u7684\u529f\u80fd\u548c\u7279\u6027\u3002scuttle\u53ef\u4ee5\u5c06\u9ad8\u7ef4\u7684scRNA-seq\u6570\u636e\u8f6c\u5316\u4e3a\u4f4e\u7ef4\u7a7a\u95f4\uff0c\u5982\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u548ct\u5206\u5e03\u968f\u673a\u90bb\u57df\u5d4c\u5165\uff08t-SNE\uff09\u7b49\u65b9\u6cd5\uff0c\u4ece\u800c\u5728\u4e8c\u7ef4\u6216\u4e09\u7ef4\u5e73\u9762\u4e0a\u5448\u73b0\u7ec6\u80de\u7684\u5206\u5e03\u3002\u8fd9\u4f7f\u5f97\u7814\u7a76\u4eba\u5458\u80fd\u591f\u66f4\u51c6\u786e\u5730\u8bc6\u522b\u548c\u7406\u89e3\u7ec6\u80de\u7684\u805a\u7c7b\u6a21\u5f0f\u3001\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u4ee5\u53ca\u5728\u53d1\u80b2\u8fc7\u7a0b\u4e2d\u7ec6\u80de\u72b6\u6001\u7684\u53d8\u5316\u3002<\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u901a\u8fc7\u8fd9\u79cd\u964d\u7ef4\u548c\u53ef\u89c6\u5316\u7684\u624b\u6bb5\uff0cscuttle\u8d4b\u4e88\u4e86\u7814\u7a76\u4eba\u5458\u6df1\u5165\u63a2\u7d22\u7ec6\u80de\u591a\u6837\u6027\u7684\u80fd\u529b\u3002\u65e0\u8bba\u662f\u5728\u7814\u7a76\u6b63\u5e38\u7ec4\u7ec7\u4e2d\u7684\u7ec6\u80de\u5f02\u8d28\u6027\uff0c\u8fd8\u662f\u5728\u63ed\u793a\u7ec4\u7ec7\u53d1\u80b2\u8fc7\u7a0b\u4e2d\u7684\u7ec6\u80de\u5206\u5316\uff0cscuttle\u90fd\u4e3a\u751f\u7269\u5b66\u5bb6\u63d0\u4f9b\u4e86\u4e00\u4e2a\u6709\u529b\u7684\u5de5\u5177\u3002\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u901a\u8fc7\u89c2\u5bdf\u805a\u7c7b\u56fe\u3001t-SNE\u56fe\u4ee5\u53ca\u5176\u4ed6\u964d\u7ef4\u53ef\u89c6\u5316\u56fe\u8868\uff0c\u66f4\u6e05\u6670\u5730\u89c2\u5bdf\u4e0d\u540c\u7ec6\u80de\u7684\u5206\u5e03\u548c\u8d8b\u52bf\u3002\u8fd9\u79cd\u6df1\u5165\u7684\u7406\u89e3\u5c06\u4e3a\u751f\u7269\u5b66\u5bb6\u4eec\u6784\u5efa\u7ec6\u80de\u7c7b\u578b\u5206\u7c7b\u3001\u63ed\u793a\u53d1\u80b2\u8f68\u8ff9\u4ee5\u53ca\u53d1\u73b0\u65b0\u7684\u7ec6\u80de\u4e9a\u7fa4\u63d0\u4f9b\u6781\u5927\u7684\u5e2e\u52a9\u3002<\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">II. \u63ed\u793a\u75be\u75c5\u673a\u5236\u548c\u836f\u7269\u9776\u70b9<\/span><\/p>\n<p><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-size: 15px;font-variant: normal;text-transform: none;\">\u9664\u4e86\u5728\u63a2\u7d22\u6b63\u5e38\u751f\u7269\u5b66\u8fc7\u7a0b\u4e2d\u7684\u5e94\u7528\uff0cscuttle\u5728\u7814\u7a76\u75be\u75c5\u673a\u5236\u548c\u836f\u7269\u7814\u53d1\u9886\u57df\u4e5f\u626e\u6f14\u7740\u91cd\u8981\u89d2\u8272\u3002\u75be\u75c5\u5f80\u5f80\u8868\u73b0\u4e3a\u7ec6\u80de\u72b6\u6001\u7684\u5f02\u5e38\uff0c\u800c\u8fd9\u4e9b\u53d8\u5316\u53ef\u80fd\u5728\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\u4e0a\u5f97\u5230\u4f53\u73b0\u3002\u901a\u8fc7\u6bd4\u8f83\u75c5\u6001\u7ec6\u80de\u548c\u6b63\u5e38\u7ec6\u80de\u7684\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\uff0c\u6211\u4eec\u53ef\u4ee5\u8bc6\u522b\u51fa\u4e0e\u75be\u75c5\u53d1\u5c55\u5bc6\u5207\u76f8\u5173\u7684\u5173\u952e\u57fa\u56e0\u3002scuttle\u7684\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\u5206\u6790\u529f\u80fd\u4e3a\u7814\u7a76\u4eba\u5458\u63d0\u4f9b\u4e86\u4e00\u4e2a\u6709\u6548\u7684\u65b9\u5f0f\u6765\u9274\u5b9a\u8fd9\u4e9b\u57fa\u56e0\uff0c\u4ece\u800c\u6df1\u5165\u4e86\u89e3\u75be\u75c5\u7684\u5206\u5b50\u673a\u5236\u3002<\/span>    <\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5728\u8fd9\u4e2a\u8fc7\u7a0b\u4e2d\uff0c\u964d\u7ef4\u548c\u53ef\u89c6\u5316\u7684\u6280\u672f\u4e5f\u53d1\u6325\u7740\u91cd\u8981\u4f5c\u7528\u3002\u901a\u8fc7\u7ed8\u5236\u57fa\u56e0\u8868\u8fbe\u7684\u70ed\u56fe\u3001\u7bb1\u7ebf\u56fe\u7b49\u56fe\u8868\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u76f4\u89c2\u5730\u5c55\u793a\u4e0d\u540c\u57fa\u56e0\u5728\u75be\u75c5\u548c\u6b63\u5e38\u6837\u672c\u4e4b\u95f4\u7684\u5dee\u5f02\u3002\u8fd9\u4e0d\u4ec5\u6709\u52a9\u4e8e\u9a8c\u8bc1\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u53ef\u9760\u6027\uff0c\u8fd8\u80fd\u591f\u63ed\u793a\u5b83\u4eec\u5728\u7ec6\u80de\u7c7b\u578b\u548c\u901a\u8def\u8c03\u63a7\u65b9\u9762\u7684\u6f5c\u5728\u529f\u80fd\u3002\u8fd9\u79cd\u5206\u6790\u548c\u53ef\u89c6\u5316\u7684\u7ed3\u5408\uff0c\u4e3a\u5bfb\u627e\u6f5c\u5728\u7684\u6cbb\u7597\u9776\u70b9\u548c\u836f\u7269\u5f00\u53d1\u63d0\u4f9b\u4e86\u6709\u529b\u7684\u652f\u6301\uff0c\u4e3a\u75be\u75c5\u7814\u7a76\u5e26\u6765\u4e86\u65b0\u7684\u5e0c\u671b\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-size: 15px;font-variant: normal;text-transform: none;\">III. <\/span><span style=\"font-size: 15px;font-variant: normal;text-transform: none;\">\u63ed\u793a\u75be\u75c5\u7684\u5206\u5b50\u673a\u5236\u4e0e\u53d1\u5c55<\/span><\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u53e6\u4e00\u4e2a\u4f7fscuttle\u5907\u53d7\u5173\u6ce8\u7684\u9886\u57df\u662f\u5728\u75be\u75c5\u7814\u7a76\u4e2d\u7684\u5e94\u7528\u3002\u75be\u75c5\u7684\u53d1\u751f\u5f80\u5f80\u4f34\u968f\u7740\u7ec6\u80de\u5185\u57fa\u56e0\u8868\u8fbe\u7684\u6539\u53d8\uff0c\u800c\u8fd9\u4e9b\u53d8\u5316\u53ef\u4ee5\u901a\u8fc7\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6280\u672f\u6765\u7cbe\u786e\u6355\u6349\u548c\u5206\u6790\u3002\u5728\u8fd9\u65b9\u9762\uff0cscuttle\u5c55\u73b0\u51fa\u5f3a\u5927\u7684\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\u5206\u6790\u529f\u80fd\uff0c\u5b83\u4e3a\u7814\u7a76\u4eba\u5458\u63d0\u4f9b\u4e86\u4e00\u4e2a\u6709\u529b\u5de5\u5177\uff0c\u53ef\u4ee5\u5728\u7ec6\u80de\u5c42\u9762\u4e0a\u8bc6\u522b\u4e0d\u540c\u72b6\u6001\u4e0b\u7684\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\u3002\u901a\u8fc7\u6bd4\u8f83\u6b63\u5e38\u548c\u5f02\u5e38\u7ec6\u80de\u7684\u57fa\u56e0\u8868\u8fbe\uff0c\u6211\u4eec\u80fd\u591f\u9274\u5b9a\u5728\u7279\u5b9a\u75be\u75c5\u673a\u5236\u4e2d\u8d77\u5173\u952e\u4f5c\u7528\u7684\u57fa\u56e0\uff0c\u8fdb\u800c\u6df1\u5165\u63a2\u7a76\u75be\u75c5\u7684\u5206\u5b50\u673a\u5236\u3002<\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">scuttle\u7684\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\u5206\u6790\u4e0d\u4ec5\u5e2e\u52a9\u6211\u4eec\u53d1\u73b0\u5728\u75be\u75c5\u4e2d\u8868\u8fbe\u6c34\u5e73\u663e\u8457\u6539\u53d8\u7684\u57fa\u56e0\uff0c\u8fd8\u80fd\u591f\u5e2e\u52a9\u6211\u4eec\u7406\u89e3\u8fd9\u4e9b\u57fa\u56e0\u5728\u75be\u75c5\u53d1\u5c55\u4e2d\u7684\u4f5c\u7528\u3002\u901a\u8fc7\u5bf9\u8fd9\u4e9b\u5dee\u5f02\u57fa\u56e0\u7684\u529f\u80fd\u3001\u901a\u8def\u548c\u76f8\u4e92\u5173\u7cfb\u7684\u6df1\u5165\u7814\u7a76\uff0c\u6211\u4eec\u53ef\u4ee5\u63ed\u793a\u51fa\u75be\u75c5\u7684\u6f5c\u5728\u673a\u5236\uff0c\u4e3a\u75be\u75c5\u7684\u9884\u9632\u3001\u8bca\u65ad\u548c\u6cbb\u7597\u63d0\u4f9b\u6709\u529b\u652f\u6301\u3002\u7279\u522b\u662f\u5728\u5bfb\u627e\u6cbb\u7597\u9776\u70b9\u548c\u5f00\u53d1\u65b0\u836f\u65b9\u9762\uff0cscuttle\u4e3a\u7814\u7a76\u4eba\u5458\u63d0\u4f9b\u4e86\u4e00\u4e2a\u5168\u9762\u7684\u89c6\u89d2\uff0c\u4f7f\u4ed6\u4eec\u80fd\u591f\u66f4\u7cbe\u51c6\u5730\u5e72\u9884\u75be\u75c5\u8fc7\u7a0b\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-size: 15px;font-variant: normal;text-transform: none;\">IV. <\/span><span style=\"font-size: 15px;font-variant: normal;text-transform: none;\">\u901a\u8fc7\u7ec6\u80de\u5c42\u9762\u7684\u5206\u6790\u63ed\u793a\u75be\u75c5\u7684\u590d\u6742\u6027<\/span><\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u75be\u75c5\u5f80\u5f80\u662f\u4e00\u4e2a\u6781\u5176\u590d\u6742\u7684\u8fc7\u7a0b\uff0c\u6d89\u53ca\u591a\u79cd\u7ec6\u80de\u7c7b\u578b\u3001\u901a\u8def\u548c\u4fe1\u53f7\u8c03\u63a7\u7f51\u7edc\u7684\u7d0a\u4e71\u3002\u901a\u8fc7scuttle\u7684\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\u5206\u6790\u529f\u80fd\uff0c\u6211\u4eec\u80fd\u591f\u5c06\u5206\u5b50\u6539\u53d8\u5f15\u8d77\u7684\u7ec6\u80de\u5c42\u9762\u7684\u54cd\u5e94\u66f4\u52a0\u8be6\u7ec6\u5730\u6355\u6349\u5230\u3002\u8fd9\u4e3a\u6211\u4eec\u63d0\u4f9b\u4e86\u4e00\u4e2a\u6df1\u5165\u7814\u7a76\u75be\u75c5\u53d1\u5c55\u7684\u7a97\u53e3\uff0c\u8ba9\u6211\u4eec\u80fd\u591f\u770b\u5230\u7ec6\u80de\u5185\u90e8\u7684\u53d8\u5316\uff0c\u7406\u89e3\u8fd9\u4e9b\u53d8\u5316\u5982\u4f55\u5f71\u54cd\u6574\u4f53\u7684\u75be\u75c5\u8fc7\u7a0b\u3002<\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u66f4\u8fdb\u4e00\u6b65\u5730\uff0cscuttle\u7684\u53ef\u89c6\u5316\u529f\u80fd\u4f7f\u5f97\u8fd9\u79cd\u7ec6\u80de\u5c42\u9762\u7684\u5206\u6790\u66f4\u52a0\u76f4\u89c2\u548c\u6613\u4e8e\u89e3\u91ca\u3002\u901a\u8fc7\u7ed8\u5236\u5dee\u5f02\u57fa\u56e0\u7684\u70ed\u56fe\u3001\u7bb1\u7ebf\u56fe\u7b49\u56fe\u8868\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u76f4\u89c2\u5730\u5c55\u793a\u8fd9\u4e9b\u57fa\u56e0\u5728\u4e0d\u540c\u72b6\u6001\u4e0b\u7684\u8868\u8fbe\u6c34\u5e73\uff0c\u63ed\u793a\u5b83\u4eec\u4e4b\u95f4\u7684\u5173\u7cfb\u548c\u8d8b\u52bf\u3002\u8fd9\u79cd\u7efc\u5408\u5206\u6790\u548c\u53ef\u89c6\u5316\u7684\u65b9\u6cd5\uff0c\u4e0d\u4ec5\u6709\u52a9\u4e8e\u9a8c\u8bc1\u5206\u6790\u7684\u53ef\u9760\u6027\uff0c\u8fd8\u80fd\u591f\u4e3a\u8fdb\u4e00\u6b65\u7684\u5b9e\u9a8c\u8bbe\u8ba1\u548c\u673a\u5236\u63a2\u7a76\u63d0\u4f9b\u6709\u529b\u7684\u6307\u5bfc\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-size: 15px;font-variant: normal;text-transform: none;\">V. <\/span><span style=\"font-size: 15px;font-variant: normal;text-transform: none;\">\u5f3a\u5927\u7684\u6570\u636e\u53ef\u89c6\u5316\u80fd\u529b<\/span><\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u9664\u4e86\u4e30\u5bcc\u7684\u5206\u6790\u529f\u80fd\uff0cscuttle\u7684\u53e6\u4e00\u4e2a\u5f15\u4eba\u6ce8\u76ee\u7684\u7279\u70b9\u662f\u5176\u5353\u8d8a\u7684\u6570\u636e\u53ef\u89c6\u5316\u80fd\u529b\u3002\u5728\u751f\u7269\u4fe1\u606f\u5b66\u9886\u57df\uff0c\u6570\u636e\u7684\u53ef\u89c6\u5316\u662f\u5c06\u5206\u6790\u7ed3\u679c\u4f20\u9012\u7ed9\u4ed6\u4eba\u3001\u53d1\u73b0\u6a21\u5f0f\u5e76\u4ea7\u751f\u65b0\u7684\u6d1e\u5bdf\u529b\u7684\u5173\u952e\u9014\u5f84\u3002scuttle\u5728\u8fd9\u4e00\u9886\u57df\u53d1\u6325\u7740\u91cd\u8981\u4f5c\u7528\uff0c\u4e3a\u7528\u6237\u63d0\u4f9b\u4e86\u591a\u79cd\u56fe\u8868\u7c7b\u578b\uff0c\u5982\u6563\u70b9\u56fe\u3001\u7bb1\u7ebf\u56fe\u3001\u70ed\u56fe\u7b49\uff0c\u4f7f\u5f97\u590d\u6742\u7684\u6570\u636e\u548c\u5206\u6790\u7ed3\u679c\u53ef\u4ee5\u4ee5\u76f4\u89c2\u7684\u65b9\u5f0f\u5448\u73b0\uff0c\u66f4\u5bb9\u6613\u88ab\u751f\u7269\u5b66\u5bb6\u3001\u7814\u7a76\u4eba\u5458\u548c\u89c2\u4f17\u7406\u89e3\u548c\u89e3\u91ca\u3002<\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u901a\u8fc7\u53ef\u89c6\u5316\uff0c\u7528\u6237\u53ef\u4ee5\u76f4\u89c2\u5730\u89c2\u5bdf\u6570\u636e\u4e2d\u7684\u6a21\u5f0f\u548c\u8d8b\u52bf\uff0c\u4ece\u800c\u66f4\u597d\u5730\u7406\u89e3\u6570\u636e\u6240\u8574\u542b\u7684\u4fe1\u606f\u3002\u4f8b\u5982\uff0c\u7ed8\u5236\u6563\u70b9\u56fe\u53ef\u4ee5\u5c55\u793a\u4e0d\u540c\u7ec6\u80de\u7684\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\uff0c\u63ed\u793a\u7ec6\u80de\u4e4b\u95f4\u7684\u76f8\u4f3c\u6027\u548c\u5dee\u5f02\u6027\u3002\u7bb1\u7ebf\u56fe\u5219\u80fd\u591f\u6e05\u6670\u5730\u663e\u793a\u57fa\u56e0\u8868\u8fbe\u7684\u5206\u5e03\u548c\u5dee\u5f02\uff0c\u5e2e\u52a9\u7528\u6237\u8bc6\u522b\u663e\u8457\u7684\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u3002\u53e6\u5916\uff0c\u70ed\u56fe\u53ef\u4ee5\u5c06\u57fa\u56e0\u7684\u8868\u8fbe\u6c34\u5e73\u7f16\u7801\u4e3a\u989c\u8272\uff0c\u5c06\u5927\u91cf\u6570\u636e\u4ee5\u76f4\u89c2\u7684\u65b9\u5f0f\u5c55\u793a\u51fa\u6765\uff0c\u5e2e\u52a9\u7528\u6237\u5feb\u901f\u8bc6\u522b\u5728\u4e0d\u540c\u7ec6\u80de\u6216\u6761\u4ef6\u4e0b\u7684\u8868\u8fbe\u6a21\u5f0f\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-size: 15px;font-variant: normal;text-transform: none;\">VI. <\/span><span style=\"font-size: 15px;font-variant: normal;text-transform: none;\">\u4fc3\u8fdb\u4fe1\u606f\u63d0\u53d6\u548c\u65b0\u5047\u8bbe\u7684\u751f\u6210<\/span><\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u6570\u636e\u53ef\u89c6\u5316\u4e0d\u4ec5\u4ec5\u662f\u5c06\u6570\u636e\u5c55\u793a\u51fa\u6765\uff0c\u66f4\u662f\u5e2e\u52a9\u7528\u6237\u4ece\u6570\u636e\u4e2d\u63d0\u53d6\u6709\u4ef7\u503c\u7684\u4fe1\u606f\u3001\u4ea7\u751f\u65b0\u7684\u79d1\u5b66\u5047\u8bbe\u7684\u8fc7\u7a0b\u3002scuttle\u7684\u56fe\u8868\u4e0d\u4ec5\u7f8e\u89c2\u6613\u61c2\uff0c\u66f4\u80fd\u5e2e\u52a9\u7528\u6237\u5c06\u6570\u636e\u4e2d\u7684\u7ec6\u5fae\u53d8\u5316\u548c\u5173\u8054\u5173\u7cfb\u5feb\u901f\u6355\u6349\u5230\u3002\u4f8b\u5982\uff0c\u5728\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\u5206\u6790\u4e2d\uff0c\u901a\u8fc7\u7ed8\u5236\u5dee\u5f02\u57fa\u56e0\u7684\u7bb1\u7ebf\u56fe\uff0c\u7528\u6237\u53ef\u4ee5\u76f4\u89c2\u5730\u53d1\u73b0\u5728\u4e0d\u540c\u6761\u4ef6\u4e0b\u57fa\u56e0\u8868\u8fbe\u7684\u5206\u5e03\u5dee\u5f02\uff0c\u8fdb\u800c\u63a8\u6d4b\u8fd9\u4e9b\u57fa\u56e0\u5728\u7ec6\u80de\u8fc7\u7a0b\u4e2d\u7684\u6f5c\u5728\u4f5c\u7528\u3002<\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u6570\u636e\u53ef\u89c6\u5316\u8fd8\u80fd\u591f\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u4ece\u4e0d\u540c\u89d2\u5ea6\u5ba1\u89c6\u6570\u636e\uff0c\u4ea7\u751f\u65b0\u7684\u7814\u7a76\u5047\u8bbe\u3002\u5f53\u6211\u4eec\u5c06\u6570\u636e\u53ef\u89c6\u5316\u4e3a\u56fe\u8868\u65f6\uff0c\u6211\u4eec\u53ef\u80fd\u4f1a\u6ce8\u610f\u5230\u4e4b\u524d\u5ffd\u7565\u7684\u6a21\u5f0f\u3001\u8d8b\u52bf\u6216\u5f02\u5e38\u3002\u8fd9\u79cd\u6d1e\u5bdf\u529b\u53ef\u4ee5\u5f15\u5bfc\u7814\u7a76\u4eba\u5458\u8fdb\u4e00\u6b65\u6df1\u5165\u63a2\u7d22\uff0c\u63d0\u51fa\u65b0\u7684\u79d1\u5b66\u95ee\u9898\uff0c\u4ee5\u53ca\u8bbe\u8ba1\u66f4\u6709\u9488\u5bf9\u6027\u7684\u5b9e\u9a8c\u65b9\u6848\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-size: 15px;font-variant: normal;text-transform: none;\">\u8981\u4f7f\u7528<\/span><span style=\"font-size: 15px;font-variant: normal;text-transform: none;\">scuttle<\/span><span style=\"font-size: 15px;font-variant: normal;text-transform: none;\">\u5305\uff0c\u53ef\u4ee5\u5728R\u4e2d\u4f7f\u7528\u4ee5\u4e0b\u547d\u4ee4\u8fdb\u884c\u5b89\u88c5\u548c\u52a0\u8f7d\uff1a<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">><\/span> install.packages(<span class=\"code-snippet__string\">\"scuttle\"<\/span>) <span class=\"code-snippet__comment\">#\u5b89\u88c5scuttle\u8bed\u8a00\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">><\/span> library(scuttle) <span class=\"code-snippet__comment\">#\u52a0\u8f7d\u8bed\u8a00\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\">scuttle\u7684\u6838\u5fc3\u529f\u80fd\u5305\u62ec\uff1a<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u8d28\u91cf\u63a7\u5236\u548c\u9884\u5904\u7406\u5728\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\u5206\u6790\u4e2d\u626e\u6f14\u7740\u81f3\u5173\u91cd\u8981\u7684\u89d2\u8272\u3002\u7531\u4e8e\u6280\u672f\u566a\u58f0\u548c\u6279\u6b21\u6548\u5e94\u7684\u5b58\u5728\uff0c\u8fd9\u4e9b\u6570\u636e\u5f80\u5f80\u9700\u8981\u7ecf\u8fc7\u4e25\u683c\u7684\u5904\u7406\uff0c\u4ee5\u786e\u4fdd\u540e\u7eed\u5206\u6790\u7684\u51c6\u786e\u6027\u548c\u53ef\u9760\u6027\u3002scuttle\u4f5c\u4e3a\u4e00\u6b3e\u5f3a\u5927\u7684\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u5de5\u5177\uff0c\u4e13\u6ce8\u4e8e\u89e3\u51b3\u8fd9\u4e9b\u6311\u6218\u3002<\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5728\u8d28\u91cf\u63a7\u5236\u65b9\u9762\uff0cscuttle\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u529f\u80fd\uff0c\u53ef\u4ee5\u5e2e\u52a9\u7528\u6237\u8bc6\u522b\u548c\u6392\u9664\u4f4e\u8d28\u91cf\u7684\u7ec6\u80de\u548c\u57fa\u56e0\u3002\u901a\u8fc7\u6d88\u9664\u8fd9\u4e9b\u95ee\u9898\u6570\u636e\u70b9\uff0c\u7528\u6237\u80fd\u591f\u83b7\u5f97\u66f4\u5e72\u51c0\u3001\u53ef\u9760\u7684\u6570\u636e\u96c6\uff0c\u4e3a\u540e\u7eed\u5206\u6790\u5960\u5b9a\u575a\u5b9e\u7684\u57fa\u7840\u3002\u6b64\u5916\uff0cscuttle\u8fd8\u80fd\u591f\u6821\u6b63\u6279\u6b21\u6548\u5e94\uff0c\u8fd9\u662f\u7279\u522b\u91cd\u8981\u7684\uff0c\u56e0\u4e3a\u6279\u6b21\u6548\u5e94\u53ef\u80fd\u5bfc\u81f4\u6570\u636e\u96c6\u4e2d\u5b58\u5728\u4e0d\u5fc5\u8981\u7684\u53d8\u5f02\uff0c\u5f71\u54cd\u5bf9\u751f\u7269\u5b66\u73b0\u8c61\u7684\u51c6\u786e\u7406\u89e3\u3002<\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u7ec6\u80de\u805a\u7c7b\u548c\u964d\u7ef4\u5206\u6790\u662f\u5355\u7ec6\u80de\u6570\u636e\u63a2\u7d22\u548c\u7406\u89e3\u7684\u5173\u952e\u6b65\u9aa4\u3002scuttle\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u5f3a\u5927\u7684\u805a\u7c7b\u7b97\u6cd5\uff0c\u4f7f\u7528\u6237\u80fd\u591f\u5c06\u76f8\u4f3c\u7684\u7ec6\u80de\u5f52\u4e3a\u540c\u4e00\u7c7b\u522b\uff0c\u4ece\u800c\u63ed\u793a\u51fa\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u4e4b\u95f4\u7684\u5173\u7cfb\u3002\u6b64\u5916\uff0c\u964d\u7ef4\u5206\u6790\u5de5\u5177\u5982\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u548ct\u5206\u5e03\u968f\u673a\u90bb\u57df\u5d4c\u5165\uff08t-SNE\uff09\u80fd\u591f\u5c06\u9ad8\u7ef4\u6570\u636e\u6620\u5c04\u5230\u4e8c\u7ef4\u7a7a\u95f4\uff0c\u4f7f\u7528\u6237\u80fd\u591f\u5728\u76f4\u89c2\u7684\u56fe\u5f62\u4e2d\u89c2\u5bdf\u6570\u636e\u7684\u5206\u5e03\u548c\u7ed3\u6784\u3002<\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\u5206\u6790\u662f\u4e86\u89e3\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u6216\u6761\u4ef6\u4e0b\u57fa\u56e0\u8868\u8fbe\u53d8\u5316\u7684\u5173\u952e\u3002scuttle\u4f7f\u7528\u6237\u80fd\u591f\u6bd4\u8f83\u4e0d\u540c\u60c5\u51b5\u4e0b\u7684\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\uff0c\u4ece\u800c\u8bc6\u522b\u51fa\u5728\u7279\u5b9a\u751f\u7269\u5b66\u8fc7\u7a0b\u4e2d\u8d77\u5173\u952e\u4f5c\u7528\u7684\u57fa\u56e0\u3002\u8fd9\u6709\u52a9\u4e8e\u6df1\u5165\u7406\u89e3\u7ec6\u80de\u5728\u4e0d\u540c\u6761\u4ef6\u4e0b\u7684\u529f\u80fd\u548c\u54cd\u5e94\u3002<\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5bf9\u4e8e\u53d1\u80b2\u8fc7\u7a0b\u7684\u7814\u7a76\uff0c\u8f68\u8ff9\u5206\u6790\u662f\u4e00\u9879\u5f3a\u5927\u7684\u5de5\u5177\u3002scuttle\u80fd\u591f\u5e2e\u52a9\u7528\u6237\u6784\u5efa\u7ec6\u80de\u72b6\u6001\u7684\u53d1\u5c55\u8f68\u8ff9\uff0c\u4ece\u800c\u63ed\u793a\u7ec6\u80de\u7684\u5206\u5316\u548c\u6f14\u5316\u8fc7\u7a0b\u3002\u8fd9\u5bf9\u4e8e\u7406\u89e3\u751f\u7269\u5b66\u4e2d\u52a8\u6001\u7684\u53d8\u5316\u548c\u53d1\u5c55\u81f3\u5173\u91cd\u8981\u3002<\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">scuttle\u5185\u7f6e\u4e86\u4e30\u5bcc\u7684\u6570\u636e\u53ef\u89c6\u5316\u529f\u80fd\uff0c\u4f7f\u7528\u6237\u80fd\u591f\u4ee5\u76f4\u89c2\u7684\u65b9\u5f0f\u5c55\u793a\u4ed6\u4eec\u7684\u5206\u6790\u7ed3\u679c\u3002\u65e0\u8bba\u662f\u901a\u8fc7\u70ed\u56fe\u3001\u6563\u70b9\u56fe\u8fd8\u662f\u8f68\u8ff9\u56fe\uff0c\u7528\u6237\u90fd\u80fd\u591f\u66f4\u6e05\u6670\u5730\u4f20\u8fbe\u4ed6\u4eec\u7684\u53d1\u73b0\uff0c\u5e2e\u52a9\u5176\u4ed6\u7814\u7a76\u4eba\u5458\u66f4\u597d\u5730\u7406\u89e3\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u7684\u7ed3\u679c\u3002<\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u793a\u4f8b\uff1a<\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5f53\u4f7f\u7528scuttle\u5305\u8fdb\u884c\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\u5206\u6790\u65f6\uff0c\u6211\u4eec\u53ef\u4ee5\u4ee5\u4e00\u4e2a\u7b80\u5355\u7684\u793a\u4f8b\u4e3a\u4f8b\uff0c\u4f7f\u7528\u4e00\u4e2a\u5305\u542b\u7ec6\u80de\u7c7b\u578b\u548c\u57fa\u56e0\u8868\u8fbe\u4fe1\u606f\u7684\u6570\u636e\u96c6\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span> \u5b89\u88c5\u548c\u52a0\u8f7dscuttle\u5305<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">><\/span> install.packages(<span class=\"code-snippet__string\">\"scuttle\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">><\/span> library(scuttle)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span> \u79fb\u9664\u4f4e\u57fa\u56e0\u8868\u8fbe\u7684\u7ec6\u80de<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">><\/span> scRNA_data_filtered <- filterCells(scRNA_data, min_genes = 200)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span> \u6821\u6b63\u6279\u6b21\u6548\u5e94<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">><\/span> scRNA_data_corrected <- correctBatchEffect(scRNA_data_filtered, batch_var = <span class=\"code-snippet__string\">\"Batch\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span> \u8fdb\u884cPCA\u964d\u7ef4<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">><\/span> scRNA_data_pca <- runPCA(scRNA_data_corrected, ncomponents = 30)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span> \u8fdb\u884ct-SNE\u964d\u7ef4<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">><\/span> scRNA_data_tsne <- runTSNE(scRNA_data_pca)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span> \u6839\u636e\u7ec6\u80de\u7c7b\u578b\u6807\u8bc6\uff0c\u8fdb\u884c\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\u5206\u6790<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">><\/span> de_genes <- differentialGeneTest(scRNA_data_corrected, grouping_var = <span class=\"code-snippet__string\">\"CellType\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span> \u9009\u53d6\u663e\u8457\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">><\/span> top_de_genes <- subset(de_genes, padj < 0.05)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span> \u7ed8\u5236\u7ec6\u80de\u805a\u7c7b\u7684t-SNE\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">><\/span> plotTSNE(scRNA_data_tsne, color_by = <span class=\"code-snippet__string\">\"CellType\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p><o:page><\/o:page><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.7138888888888889\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_y1BmoKbw4xo5m0TRUicchjj1dEFJow.png?resize=553%2C395\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOezg1r8icRAHtZdf4wwGpCyo402y1BmoKbw4xo5m0TRUicchjj1dEFJow\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"395 .5400085449219\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><span style=\"letter-spacing: 0.034em;\"><\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span> \u7ed8\u5236\u663e\u8457\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u70ed\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">><\/span> plotHeatmap(scRNA_data_corrected, genes = top_de_genes<span class=\"code-snippet__variable\">$gene_id<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"1.0158371040723981\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_UDSv8uptvIiadwAPicPiceYUJo65hg.png?resize=425%2C431\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOMAIsAw1GZbIZNpp8WYfqKbDkV9yUDSv8uptvIiadwAPicPiceYUJo65hg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"442\" height=\"431 .94000244140625\" width=\"425 .2099609375\" data-recalc-dims=\"1\" \/><span style=\"letter-spacing: 0.034em;\"><\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span> \u7ed8\u5236\u7ec6\u80de\u7c7b\u578b\u95f4\u7684\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\u7bb1\u7ebf\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">><\/span> plotGeneBoxplot(scRNA_data_corrected, genes = top_de_genes<span class=\"code-snippet__variable\">$gene_id<\/span>, grouping_var = <span class=\"code-snippet__string\">\"CellType\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.5186915887850467\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_DAia0j2SQPNxl2YhFYUoQpXNFWnKeQ.png?resize=512%2C265\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOAibKQLxcTzWCSsoNReCBeLBJu3DAia0j2SQPNxl2YhFYUoQpXNFWnKeQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"428\" height=\"265 .739990234375\" width=\"512 .3400268554688\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><o:p><\/o:p><\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">\u901a\u8fc7\u8fd9\u4e2a\u793a\u4f8b\uff0c\u6211\u4eec\u53ef\u4ee5\u770b\u5230scuttle\u5305\u7684\u6838\u5fc3\u529f\u80fd\u5728\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\u5206\u6790\u4e2d\u7684\u5e94\u7528\u3002\u4ece\u8d28\u91cf\u63a7\u5236\u3001\u7ec6\u80de\u805a\u7c7b\u3001\u964d\u7ef4\u5206\u6790\u5230\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\u5206\u6790\uff0cscuttle\u4e3a\u6211\u4eec\u63d0\u4f9b\u4e86\u4e00\u5957\u5168\u9762\u7684\u5de5\u5177\uff0c\u5e2e\u52a9\u6211\u4eec\u66f4\u597d\u5730\u7406\u89e3\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6570\u636e\u80cc\u540e\u7684\u751f\u7269\u5b66\u4fe1\u606f\u3002\u53ef\u89c6\u5316\u56fe\u8868\u5982t-SNE\u56fe\u3001\u70ed\u56fe\u548c\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\u7bb1\u7ebf\u56fe\u5219\u4f7f\u5f97\u5206\u6790\u7ed3\u679c\u66f4\u52a0\u76f4\u89c2\u548c\u6613\u4e8e\u89e3\u91ca\u3002\u8fd9\u4e2a\u7b80\u5355\u7684\u793a\u4f8b\u5c55\u793a\u4e86scuttle\u5728\u751f\u7269\u5b66\u7814\u7a76\u4e2d\u7684\u5b9e\u9645\u5e94\u7528\uff0c\u4e3a\u6211\u4eec\u63a2\u7d22\u7ec6\u80de\u591a\u6837\u6027\u3001\u53d1\u80b2\u8fc7\u7a0b\u548c\u75be\u75c5\u673a\u5236\u63d0\u4f9b\u4e86\u5f3a\u6709\u529b\u7684\u652f\u6301\u3002<\/span><\/p>\n<p><span 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