{"id":55104,"date":"2024-06-16T16:13:51","date_gmt":"2024-06-16T08:13:51","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55104"},"modified":"2024-06-16T16:13:51","modified_gmt":"2024-06-16T08:13:51","slug":"%e5%bf%ab%e9%80%9f%e5%88%86%e6%9e%90%e7%94%9f%e7%89%a9%e5%ba%8f%e5%88%97%e6%95%b0%e6%8d%ae%e7%9a%84%e7%a5%9e%e4%bb%99r%e8%af%ad%e8%a8%80%e5%8c%85seqinr","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55104","title":{"rendered":"\u5feb\u901f\u5206\u6790\u751f\u7269\u5e8f\u5217\u6570\u636e\u7684\u795e\u4ed9R\u8bed\u8a00\u5305seqinr"},"content":{"rendered":"<p><html><br \/>\n<head><br \/>\n<title><\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" 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span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation Mono\",Menlo,Courier,monospace}.code-snippet__comment,.code-snippet__quote{color:#afafaf;font-style:italic}.code-snippet__keyword,.code-snippet__selector-tag,.code-snippet__subst{color:#ca7d37}.code-snippet__number,.code-snippet__literal,.code-snippet__variable,.code-snippet__template-variable,.code-snippet__tag 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auto;min-width: 5%;height: auto;background-color: rgb(249, 172, 172);box-shadow: rgb(249, 172, 172) 0px 0px 5px 3px;\">\n<section style=\"text-align: center;transform: translate3d(2px, 0px, 0px);margin-top: -14px;margin-bottom: -14px;\" powered-by=\"xiumi.us\">\n<p>{ \u70b9\u51fb\u84dd\u5b57\uff0c\u5173\u6ce8\u6211\u4eec }<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">seqinr\u662f\u4e00\u4e2a\u5728R\u8bed\u8a00\u4e2d\u4f7f\u7528\u7684\u751f\u7269\u4fe1\u606f\u5b66\u5305\uff0c\u5b83\u63d0\u4f9b\u4e86\u4e00\u5957\u529f\u80fd\u5f3a\u5927\u7684\u5de5\u5177\uff0c\u7528\u4e8e\u5904\u7406\u548c\u5206\u6790\u751f\u7269\u5e8f\u5217\u6570\u636e\u3002\u65e0\u8bba\u662fDNA\u3001RNA\u8fd8\u662f\u86cb\u767d\u8d28\u5e8f\u5217\uff0cseqinr\u90fd\u80fd\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u4ece\u4e2d\u63d0\u53d6\u6709\u4ef7\u503c\u7684\u4fe1\u606f\u3002seqinr\u5305\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u51fd\u6570\uff0c\u6db5\u76d6\u4e86\u751f\u7269\u5e8f\u5217\u5206\u6790\u7684\u5404\u4e2a\u65b9\u9762\u3002<br  \/><\/p>\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<p style=\"white-space: normal;\">\u8981\u4f7f\u7528seqinr\u5305\uff0c\u53ef\u4ee5\u5728R\u4e2d\u4f7f\u7528\u4ee5\u4e0b\u547d\u4ee4\u8fdb\u884c\u5b89\u88c5\u548c\u52a0\u8f7d\uff1a<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">&gt; install.packages(<span class=\"code-snippet__string\">\"seqinr\"<\/span>) &nbsp;<span class=\"code-snippet__comment\">#\u5b89\u88c5seqinr\u8bed\u8a00\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">&gt; library(seqinr) &nbsp;<span class=\"code-snippet__comment\">#\u52a0\u8f7d\u8bed\u8a00\u5305<\/span><\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<p style=\"white-space: normal;\">\u9996\u5148\uff0c\u5b83\u5177\u6709\u8bfb\u53d6\u548c\u5199\u5165\u5e8f\u5217\u6570\u636e\u7684\u529f\u80fd\u3002\u65e0\u8bba\u662f\u4ece\u672c\u5730\u6587\u4ef6\u8fd8\u662f\u4ece\u5728\u7ebf\u6570\u636e\u5e93\uff0cseqinr\u90fd\u80fd\u8f7b\u677e\u8bfb\u53d6\u5404\u79cd\u683c\u5f0f\u7684\u5e8f\u5217\u6570\u636e\uff0c\u5982FASTA\u3001GenBank\u548cEMBL\u3002\u6b64\u5916\uff0cseqinr\u8fd8\u63d0\u4f9b\u4e86\u4e00\u4e9b\u7528\u4e8e\u5e8f\u5217\u5904\u7406\u548c\u8f6c\u6362\u7684\u51fd\u6570\u3002\u4f8b\u5982\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u4f7f\u7528seqinr\u5305\u4e2d\u7684\u51fd\u6570\u8ba1\u7b97\u5e8f\u5217\u7684\u78b1\u57fa\u7ec4\u6210\uff0c\u5305\u62ec\u78b1\u57fa\u9891\u7387\u548cGC\u542b\u91cf\u3002\u8fd9\u5bf9\u4e8e\u7406\u89e3\u5e8f\u5217\u7684\u7279\u5f81\u548c\u5c5e\u6027\u975e\u5e38\u91cd\u8981\uff0c\u56e0\u4e3a\u4e0d\u540c\u7684\u78b1\u57fa\u7ec4\u6210\u53ef\u4ee5\u63d0\u4f9b\u6709\u5173\u5e8f\u5217\u7684\u751f\u7269\u5b66\u4fe1\u606f\u3002<br  \/><\/p>\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<section><strong style=\"letter-spacing: 0.034em;text-align: justify;-webkit-text-size-adjust: 100%;\">\u793a\u4f8b\uff1a<\/strong><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u5bfc\u5165seqinr\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">&gt; library(seqinr)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u521b\u5efa\u4e00\u4e2a\u5305\u542bFASTA\u5e8f\u5217\u7684\u5b57\u7b26\u5411\u91cf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">&gt; sequence_content &lt;- <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"&gt;Sequence 1\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__string\">\"ACGTACGTACGT\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__string\">\"&gt;Sequence 2\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__string\">\"TGCAATGC\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__string\">\"&gt;Sequence 3\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__string\">\"CCCCGGGG\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u4fdd\u5b58\u5e8f\u5217\u6570\u636e\u5230\u4e34\u65f6\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">&gt; fasta_file &lt;- tempfile()<\/span><\/code><code><span class=\"code-snippet_outer\">&gt; writeLines(sequence_content, fasta_file)<\/span><\/code><code><span class=\"code-snippet_outer\"><br  \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8bfb\u53d6\u5e8f\u5217\u6570\u636e\u7684\u540d\u79f0\u548c\u5185\u5bb9<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">&gt; sequences &lt;- read.fasta(fasta_file)<\/span><\/code><code><span class=\"code-snippet_outer\"><br  \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u63d0\u53d6\u5e8f\u5217\u540d\u79f0\u548c\u5185\u5bb9<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">&gt; sequence_names &lt;- <span class=\"code-snippet__built_in\">names<\/span>(sequences)<\/span><\/code><code><span class=\"code-snippet_outer\">&gt; sequence_content &lt;- sapply(sequences, <span class=\"code-snippet__built_in\">as.character<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">&gt; print(sequence_names)<\/span><\/code><code><span class=\"code-snippet_outer\">[<span class=\"code-snippet__number\">1<\/span>] <span class=\"code-snippet__string\">\"Sequence\"<\/span> <span class=\"code-snippet__string\">\"Sequence\"<\/span> <span class=\"code-snippet__string\">\"Sequence\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">&gt; print(sequence_content)<\/span><\/code><code><span class=\"code-snippet_outer\">$Sequence<\/span><\/code><code><span class=\"code-snippet_outer\">[<span class=\"code-snippet__number\">1<\/span>] <span class=\"code-snippet__string\">\"a\"<\/span> <span class=\"code-snippet__string\">\"c\"<\/span> <span class=\"code-snippet__string\">\"g\"<\/span> <span class=\"code-snippet__string\">\"t\"<\/span> <span class=\"code-snippet__string\">\"a\"<\/span> <span class=\"code-snippet__string\">\"c\"<\/span> <span class=\"code-snippet__string\">\"g\"<\/span> <span class=\"code-snippet__string\">\"t\"<\/span> <span class=\"code-snippet__string\">\"a\"<\/span> <span class=\"code-snippet__string\">\"c\"<\/span> <span class=\"code-snippet__string\">\"g\"<\/span> <span class=\"code-snippet__string\">\"t\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br  \/><\/span><\/code><code><span class=\"code-snippet_outer\">$Sequence<\/span><\/code><code><span class=\"code-snippet_outer\">[<span class=\"code-snippet__number\">1<\/span>] <span class=\"code-snippet__string\">\"t\"<\/span> <span class=\"code-snippet__string\">\"g\"<\/span> <span class=\"code-snippet__string\">\"c\"<\/span> <span class=\"code-snippet__string\">\"a\"<\/span> <span class=\"code-snippet__string\">\"a\"<\/span> <span class=\"code-snippet__string\">\"t\"<\/span> <span class=\"code-snippet__string\">\"g\"<\/span> <span class=\"code-snippet__string\">\"c\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">$Sequence<\/span><\/code><code><span class=\"code-snippet_outer\">[<span class=\"code-snippet__number\">1<\/span>] <span class=\"code-snippet__string\">\"c\"<\/span> <span class=\"code-snippet__string\">\"c\"<\/span> <span class=\"code-snippet__string\">\"c\"<\/span> <span class=\"code-snippet__string\">\"c\"<\/span> <span class=\"code-snippet__string\">\"g\"<\/span> <span class=\"code-snippet__string\">\"g\"<\/span> <span class=\"code-snippet__string\">\"g\"<\/span> <span class=\"code-snippet__string\">\"g\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br  \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8ba1\u7b97\u5e8f\u5217\u7684\u78b1\u57fa\u7ec4\u6210<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">&gt; base_composition &lt;- count(sequence_content[[<span class=\"code-snippet__number\">1<\/span>]], wordsize = <span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">&gt; print(base_composition)<\/span><\/code><code><span class=\"code-snippet_outer\">a <span class=\"code-snippet__built_in\">c<\/span> g t<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__number\">3<\/span> <span class=\"code-snippet__number\">3<\/span> <span class=\"code-snippet__number\">3<\/span> <span class=\"code-snippet__number\">3<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br  \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8ba1\u7b97\u5e8f\u5217\u7684GC\u542b\u91cf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">&gt; gc_content &lt;- GC(sequence_content[[<span class=\"code-snippet__number\">1<\/span>]])<\/span><\/code><code><span class=\"code-snippet_outer\">&gt; print(gc_content)<\/span><\/code><code><span class=\"code-snippet_outer\">[<span class=\"code-snippet__number\">1<\/span>] <span class=\"code-snippet__number\">0.5<\/span><\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<p style=\"white-space: normal;\">\u5728\u8fd9\u4e2a\u793a\u4f8b\u4e2d\uff0c\u6211\u4eec\u9996\u5148\u4f7f\u7528read.fasta()\u51fd\u6570\u4ece\u672c\u5730\u6587\u4ef6\u4e2d\u8bfb\u53d6FASTA\u683c\u5f0f\u7684\u5e8f\u5217\u6570\u636e\u3002\u8bfb\u53d6\u540e\uff0c\u6211\u4eec\u63d0\u53d6\u4e86\u5e8f\u5217\u6570\u636e\u7684\u540d\u79f0\u548c\u5185\u5bb9\uff0c\u5e76\u5c06\u5176\u5b58\u50a8\u5728sequence_names\u548csequence_content\u53d8\u91cf\u4e2d\u3002\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u4f7f\u7528count()\u51fd\u6570\u8ba1\u7b97\u4e86\u7b2c\u4e00\u4e2a\u5e8f\u5217\u7684\u78b1\u57fa\u7ec4\u6210\u3002count()\u51fd\u6570\u63a5\u53d7\u4e00\u4e2a\u5b57\u7b26\u5411\u91cf\u4f5c\u4e3a\u8f93\u5165\uff0c\u7136\u540e\u8fd4\u56de\u4e00\u4e2a\u5305\u542b\u78b1\u57fa\u9891\u7387\u7684\u8868\u683c\u3002\u8fd9\u91cc\uff0c\u6211\u4eec\u5c06\u7b2c\u4e00\u4e2a\u5e8f\u5217\u7684\u5185\u5bb9\u4f5c\u4e3a\u8f93\u5165\uff0c\u5e76\u5c06\u7ed3\u679c\u5b58\u50a8\u5728base_composition\u53d8\u91cf\u4e2d\u3002\u6700\u540e\uff0c\u6211\u4eec\u4f7f\u7528GC()\u51fd\u6570\u8ba1\u7b97\u4e86\u7b2c\u4e00\u4e2a\u5e8f\u5217\u7684GC\u542b\u91cf\u3002GC()\u51fd\u6570\u63a5\u53d7\u4e00\u4e2a\u5b57\u7b26\u5411\u91cf\u4f5c\u4e3a\u8f93\u5165\uff0c\u5e76\u8fd4\u56de\u5e8f\u5217\u4e2dGC\u78b1\u57fa\u7684\u767e\u5206\u6bd4\u3002\u5728\u8fd9\u4e2a\u793a\u4f8b\u4e2d\uff0c\u6211\u4eec\u5c06\u7b2c\u4e00\u4e2a\u5e8f\u5217\u7684\u5185\u5bb9\u4f5c\u4e3a\u8f93\u5165\uff0c\u5e76\u5c06\u7ed3\u679c\u5b58\u50a8\u5728gc_content\u53d8\u91cf\u4e2d\u3002\u901a\u8fc7\u8fd9\u4e2a\u793a\u4f8b\uff0c\u4f60\u53ef\u4ee5\u5728R\u4e2d\u4f7f\u7528seqinr\u5305\u6765\u8bfb\u53d6\u5e8f\u5217\u6570\u636e\u3001\u63d0\u53d6\u5e8f\u5217\u540d\u79f0\u548c\u5185\u5bb9\uff0c\u5e76\u8ba1\u7b97\u5e8f\u5217\u7684\u78b1\u57fa\u7ec4\u6210\u548cGC\u542b\u91cf\u3002<\/p>\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6098081023454158\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_TyzgUP32AS2erLjOhrs56ouTRNcXBA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEXW94AvH9euLIr2w3PTfxJJLuPpoaKdCuBdMegOB2n7GtRFTyzgUP32AS2erLjOhrs56ouTRNcXBA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"938\" style=\"vertical-align: middle;width: 100%;\"  data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">seqinr\u8fd8\u5305\u542b\u4e86\u4e00\u4e9b\u7528\u4e8e\u5e8f\u5217\u6bd4\u5bf9\u548c\u641c\u7d22\u7684\u51fd\u6570\u3002\u6bd4\u5bf9\u662f\u751f\u7269\u4fe1\u606f\u5b66\u4e2d\u5e38\u7528\u7684\u6280\u672f\uff0c\u7528\u4e8e\u6bd4\u8f83\u4e0d\u540c\u5e8f\u5217\u4e4b\u95f4\u7684\u76f8\u4f3c\u6027\u548c\u5dee\u5f02\u6027\u3002seqinr\u5305\u4e2d\u7684\u51fd\u6570\u53ef\u4ee5\u6267\u884c\u591a\u79cd\u7c7b\u578b\u7684\u6bd4\u5bf9\uff0c\u5305\u62ec\u5168\u5c40\u6bd4\u5bf9\u3001\u5c40\u90e8\u6bd4\u5bf9\u548c\u591a\u5e8f\u5217\u6bd4\u5bf9\u3002\u6b64\u5916\uff0cseqinr\u8fd8\u63d0\u4f9b\u4e86\u5feb\u901f\u641c\u7d22\u7279\u5b9a\u6a21\u5f0f\u6216\u5e8f\u5217\u7247\u6bb5\u7684\u529f\u80fd\uff0c\u4ee5\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u5728\u5e8f\u5217\u6570\u636e\u4e2d\u5b9a\u4f4d\u611f\u5174\u8da3\u7684\u90e8\u5206\u3002<br  \/><\/p>\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<p style=\"white-space: normal;\">\u6b64\u5916\uff0cseqinr\u8fd8\u5305\u62ec\u4e00\u4e9b\u7528\u4e8e\u8ba1\u7b97\u5e8f\u5217\u7279\u5f81\u548c\u5c5e\u6027\u7684\u51fd\u6570\u3002\u4f8b\u5982\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u4f7f\u7528seqinr\u5305\u4e2d\u7684\u51fd\u6570\u8ba1\u7b97\u5e8f\u5217\u7684\u957f\u5ea6\u3001\u5206\u5b50\u91cf\u548c\u7b49\u7535\u70b9\u3002\u8fd9\u5bf9\u4e8e\u5206\u6790\u86cb\u767d\u8d28\u5e8f\u5217\u975e\u5e38\u6709\u7528\uff0c\u56e0\u4e3a\u8fd9\u4e9b\u7279\u5f81\u53ef\u4ee5\u63d0\u4f9b\u5173\u4e8e\u86cb\u767d\u8d28\u529f\u80fd\u548c\u7ed3\u6784\u7684\u91cd\u8981\u4fe1\u606f\u3002<\/p>\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<p style=\"white-space: normal;\"><strong>\u793a\u4f8b\uff1a<\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u86cb\u767d\u8d28\u5e8f\u5217\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">&gt; protein_sequence &lt;- <span class=\"code-snippet__string\">\"MSAGFKYKGSVRLTKGSKTLKLKKVHKTLPNKILKKLKKAKIGTVEHTGEKFFIEALYE\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8ba1\u7b97\u5e8f\u5217\u957f\u5ea6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">&gt; sequence_length &lt;- nchar(protein_sequence)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8ba1\u7b97\u5e8f\u5217\u7684\u5206\u5b50\u91cf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">&gt; molecular_weight &lt;- molecular.weight(protein_sequence)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8ba1\u7b97\u5e8f\u5217\u7684\u7b49\u7535\u70b9<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">&gt; isoelectric_point &lt;- pI(protein_sequence)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9877551020408163\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_aZwWfldKhibgMjVn6MGnPdUpZIOTMQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEXW94AvH9euLIr2w3PTfxJJhjOosuWujtNHxshCibDtCqGTYpiaZwWfldKhibgMjVn6MGnPdUpZIOTMQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"980\" style=\"vertical-align: middle;width: 100%;\"  data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<p style=\"white-space: normal;\">\u9664\u4e86\u57fa\u672c\u7684\u5e8f\u5217\u5904\u7406\u548c\u5206\u6790\u529f\u80fd\uff0cseqinr\u8fd8\u63d0\u4f9b\u4e86\u4e00\u4e9b\u9ad8\u7ea7\u529f\u80fd\uff0c\u5982\u5e8f\u5217\u6a21\u62df\u548c\u8fdb\u5316\u5206\u6790\u3002\u5e8f\u5217\u6a21\u62df\u5141\u8bb8\u7814\u7a76\u4eba\u5458\u751f\u6210\u5177\u6709\u7279\u5b9a\u5c5e\u6027\u7684\u5408\u6210\u5e8f\u5217\uff0c\u8fd9\u5bf9\u4e8e\u8bc4\u4f30\u5206\u6790\u65b9\u6cd5\u7684\u6027\u80fd\u548c\u9a8c\u8bc1\u5047\u8bbe\u975e\u5e38\u6709\u7528\u3002\u800c\u8fdb\u5316\u5206\u6790\u5219\u63d0\u4f9b\u4e86\u4e00\u4e9b\u51fd\u6570\uff0c\u7528\u4e8e\u63a8\u65ad\u5e8f\u5217\u7684\u8fdb\u5316\u5173\u7cfb\u548c\u6784\u5efa\u8fdb\u5316\u6811\uff0c\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u4e86\u89e3\u4e0d\u540c\u7269\u79cd\u4e4b\u95f4\u7684\u4eb2\u7f18\u5173\u7cfb\u3002<\/p>\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<p style=\"white-space: normal;\"><strong>\u793a\u4f8b\uff1a<\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u6a21\u62df\u5408\u6210\u5e8f\u5217<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">&gt; synthetic_sequence &lt;- s2c(simseq(n = <span class=\"code-snippet__number\">100<\/span>, alphab = <span class=\"code-snippet__string\">\"DNA\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8f93\u51fa\u5408\u6210\u5e8f\u5217<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">&gt; print(synthetic_sequence)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u63a8\u65ad\u5e8f\u5217\u7684\u8fdb\u5316\u5173\u7cfb<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">&gt; sequences &lt;- read.fasta(system.file(<span class=\"code-snippet__string\">\"sequences\"<\/span>, <span class=\"code-snippet__string\">\"example.fasta\"<\/span>, package = <span class=\"code-snippet__string\">\"seqinr\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\">&gt; evolutionary_tree &lt;- dist.alignment(sequences, model = <span class=\"code-snippet__string\">\"TN93\"<\/span>, type = <span class=\"code-snippet__string\">\"DNA\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">&gt; print(evolutionary_tree)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u6784\u5efa\u8fdb\u5316\u6811<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">&gt; phylogenetic_tree &lt;- nj(evolutionary_tree)<\/span><\/code><code><span class=\"code-snippet_outer\">&gt; print(phylogenetic_tree)<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<p style=\"white-space: normal;\">\u5728\u8fd9\u4e2a\u793a\u4f8b\u4e2d\uff0c\u6211\u4eec\u9996\u5148\u4f7f\u7528simseq()\u51fd\u6570\u6a21\u62df\u5408\u6210\u4e86\u4e00\u4e2a\u5305\u542b100\u4e2aDNA\u5e8f\u5217\u7684\u5408\u6210\u5e8f\u5217\u3002\u5408\u6210\u5e8f\u5217\u7684\u5c5e\u6027\u53ef\u4ee5\u901a\u8fc7\u8c03\u6574\u51fd\u6570\u7684\u53c2\u6570\u6765\u63a7\u5236\uff0c\u4f8b\u5982\u5e8f\u5217\u6570\u91cf\u3001\u5e8f\u5217\u957f\u5ea6\u3001\u4f7f\u7528\u7684\u78b1\u57fa\u5b57\u6bcd\u7b49\u3002\u5728\u8fd9\u91cc\uff0c\u6211\u4eec\u4f7f\u7528\u4e86\u9ed8\u8ba4\u7684\u53c2\u6570\uff0c\u5e76\u5c06\u7ed3\u679c\u5b58\u50a8\u5728synthetic_sequence\u53d8\u91cf\u4e2d\u3002\u7136\u540e\uff0c\u6211\u4eec\u4f7f\u7528read.fasta()\u51fd\u6570\u8bfb\u53d6\u4e86\u4e00\u4e2a\u5305\u542b\u591a\u4e2aDNA\u5e8f\u5217\u7684FASTA\u6587\u4ef6\u3002\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u4f7f\u7528dist.alignment()\u51fd\u6570\u6839\u636e\u5e8f\u5217\u7684\u5dee\u5f02\u6027\u63a8\u65ad\u4e86\u5e8f\u5217\u7684\u8fdb\u5316\u5173\u7cfb\u3002\u8fd9\u4e2a\u51fd\u6570\u57fa\u4e8e\u5e8f\u5217\u7684\u6bd4\u5bf9\u7ed3\u679c\u8ba1\u7b97\u5e8f\u5217\u4e4b\u95f4\u7684\u8ddd\u79bb\u77e9\u9635\uff0c\u5e76\u91c7\u7528\u4e86TN93\u6a21\u578b\u8fdb\u884c\u8fdb\u5316\u8ddd\u79bb\u7684\u4f30\u8ba1\u3002\u7ed3\u679c\u5b58\u50a8\u5728evolutionary_tree\u53d8\u91cf\u4e2d\u3002\u6700\u540e\uff0c\u6211\u4eec\u4f7f\u7528nj()\u51fd\u6570\u6784\u5efa\u4e86\u4e00\u4e2a\u8fdb\u5316\u6811\uff0c\u4f7f\u7528\u4e86evolutionary_tree\u4f5c\u4e3a\u8f93\u5165\uff0c\u5e76\u5c06\u7ed3\u679c\u5b58\u50a8\u5728phylogenetic_tree\u53d8\u91cf\u4e2d\u3002\u8fd9\u91cc\u91c7\u7528\u7684\u662f\u6700\u7b80\u5355\u7684Neighbor-Joining\u65b9\u6cd5\uff0c\u53ef\u4ee5\u6839\u636e\u8fdb\u5316\u8ddd\u79bb\u63a8\u65ad\u51fa\u7269\u79cd\u4e4b\u95f4\u7684\u4eb2\u7f18\u5173\u7cfb\u3002<br  \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.571658615136876\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_EenedT2ibDLw2GVrQp1Ka9PrMN2icQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEXW94AvH9euLIr2w3PTfxJJ6yGoOX5JYmVhlccA5NwgmiczdthEenedT2ibDLw2GVrQp1Ka9PrMN2icQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"621\" style=\"vertical-align: middle;width: 100%;\"  data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_Vbnc93dLgxiawcNWd12wlrk0NTfm6Q.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEXW94AvH9euLIr2w3PTfxJJ0xByZ5VibCOocp3L6gdvibkkMKgVbnc93dLgxiawcNWd12wlrk0NTfm6Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"480\" style=\"vertical-align: middle;width: 100%;\"  data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_VNbJWdqw1muUIvrmGLMy9kj8NZJRGg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEXW94AvH9euLIr2w3PTfxJJwwmWaBztoWpuKDpYaTSpl8XPVNbJWdqw1muUIvrmGLMy9kj8NZJRGg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"480\" style=\"vertical-align: middle;width: 100%;\"  data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u4ee5\u4e0a\u5c31\u662f\u5bf9R\u8bed\u8a00\u5305seqinr\u7684\u7b80\u5355\u4ecb\u7ecd\u5566\uff0cseqinr\u662f\u4e00\u4e2a\u529f\u80fd\u4e30\u5bcc\u7684R\u8bed\u8a00\u5305\uff0c\u4e3a\u751f\u7269\u4fe1\u606f\u5b66\u7814\u7a76\u4eba\u5458\u63d0\u4f9b\u4e86\u4e00\u5957\u5f3a\u5927\u7684\u5de5\u5177\uff0c\u7528\u4e8e\u5904\u7406\u548c\u5206\u6790\u751f\u7269\u5e8f\u5217\u6570\u636e\u3002\u65e0\u8bba\u662f\u521d\u5b66\u8005\u8fd8\u662f\u7ecf\u9a8c\u4e30\u5bcc\u7684\u4e13\u4e1a\u4eba\u58eb\uff0c\u90fd\u53ef\u4ee5\u4eceseqinr\u7684\u6613\u7528\u6027\u548c\u5e7f\u6cdb\u7684\u529f\u80fd\u4e2d\u53d7\u76ca\u3002\u901a\u8fc7\u4f7f\u7528seqinr\u5305\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u66f4\u52a0\u8f7b\u677e\u5730\u63a2\u7d22\u548c\u89e3\u91ca\u751f\u7269\u5e8f\u5217\u7684\u590d\u6742\u6027\uff0c\u4ece\u800c\u63a8\u52a8\u751f\u7269\u4fe1\u606f\u5b66\u9886\u57df\u7684\u7814\u7a76\u548c\u53d1\u5c55\u3002<br  \/><\/p>\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<p style=\"white-space: normal;\">\u5c0f\u4f19\u4f34\u4eec\uff0c\u4eca\u5929\u6709\u6ca1\u6709\u5b66\u5230\u65b0\u77e5\u8bc6\u5462\uff0c\u60f3\u8981\u7ee7\u7eed\u4e86\u89e3R\u8bed\u8a00\u5185\u5bb9\u53ef\u4ee5\u6301\u7eed\u5173\u6ce8\u5c0f\u5e08\u59b9\u54e6\uff5e\uff5e\u6216\u8005\u4e5f\u53ef\u4ee5\u5173\u6ce8\u6211\u4eec\u7684\u5b98\u7f51\u4e5f\u4f1a\u6301\u7eed\u66f4\u65b0\u7684\u54e6\uff5e http:\/\/www.biocloudservice.com\/home.html<\/p>\n<\/section>\n<section style=\"display: flex;width: 100%;flex-flow: column nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"z-index: 1;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: -42px;\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 0%;height: auto;\">\n<section style=\"transform: rotateZ(340deg) scale(0.65);margin-top: -4px;margin-bottom: -4px;transform-origin: center center;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: left;margin-bottom: 2px;transform: translate3d(-23px, 0px, 0px);\">\n<section style=\"display: inline-block;width: 20px;height: 20px;vertical-align: top;overflow: hidden;border-width: 1px;border-radius: 100%;border-style: solid;border-color: rgb(239, 135, 135);background-color: rgb(242, 203, 206);\">\n<section style=\"text-align: center;margin-top: 2px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 15px;height: 15px;vertical-align: top;overflow: hidden;border-width: 3px;border-radius: 100%;border-style: solid;border-color: rgb(255, 255, 255) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);background-color: rgb(241, 203, 206);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: rotateZ(340deg);\" powered-by=\"xiumi.us\">\n<section style=\"\">\n<section style=\"display: inline-block;width: 20px;height: 20px;vertical-align: top;overflow: hidden;border-width: 1px;border-radius: 100%;border-style: solid;border-color: rgb(239, 135, 135);background-color: rgb(242, 203, 206);\">\n<section style=\"margin-top: 2px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 15px;height: 15px;vertical-align: top;overflow: hidden;border-width: 3px;border-radius: 100%;border-style: solid;border-color: rgb(255, 255, 255) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);background-color: rgb(241, 203, 206);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;padding-right: 15px;padding-left: 15px;background-color: rgb(255, 255, 255);\">\n<section style=\"color: rgb(255, 255, 255);letter-spacing: 5px;text-shadow: rgb(239, 135, 135) 0px 1.8px 0px, rgb(239, 135, 135) 1.3px 1.3px 0px, rgb(239, 135, 135) 1.8px 0px 0px, rgb(239, 135, 135) 1.3px -1.3px 0px, rgb(239, 135, 135) 0px -1.8px 0px, rgb(239, 135, 135) -1.3px -1.3px 0px, rgb(239, 135, 135) -1.8px 0px 0px, rgb(239, 135, 135) -1.3px 1.3px 0px;\" powered-by=\"xiumi.us\">\n<p><em><span style=\"font-size: 24px;\"><strong>References:<\/strong><\/span><\/em><\/p>\n<\/section>\n<section style=\"display: flex;flex-flow: row nowrap;justify-content: center;margin-top: 3px;margin-bottom: -13px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;align-self: flex-start;border-width: 0px;margin-left: -10px;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 33px;height: 25px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 2px;border-radius: 50%;border-color: rgb(242, 166, 173) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);background-color: rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;align-self: flex-start;border-width: 0px;margin-top: -16px;margin-right: -10px;margin-left: -10px;z-index: 1;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 33px;height: 25px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 2px;border-radius: 50%;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(242, 166, 173);background-color: rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;align-self: flex-start;border-width: 0px;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 33px;height: 25px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 2px;border-radius: 50%;border-color: rgb(242, 166, 173) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);background-color: rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;align-self: flex-start;border-width: 0px;margin-top: -16px;margin-left: -10px;z-index: 1;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 33px;height: 25px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 2px;border-radius: 50%;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(242, 166, 173);background-color: rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;align-self: flex-start;border-width: 0px;margin-left: -10px;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 33px;height: 25px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 2px;border-radius: 50%;border-color: rgb(242, 166, 173) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);background-color: rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;align-self: flex-start;border-width: 0px;margin-top: -16px;margin-right: -10px;margin-left: -10px;z-index: 1;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 33px;height: 25px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 2px;border-radius: 50%;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(242, 166, 173);background-color: rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 0 0 0%;height: auto;align-self: center;\">\n<section style=\"transform: rotateZ(20deg) scale(0.65);margin-top: -4px;margin-bottom: -4px;transform-origin: center center;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: right;transform: translate3d(23px, 0px, 0px);margin-bottom: 2px;\">\n<section style=\"display: inline-block;width: 20px;height: 20px;vertical-align: top;overflow: hidden;border-width: 1px;border-radius: 100%;border-style: solid;border-color: rgb(239, 135, 135);background-color: rgb(242, 203, 206);\">\n<section style=\"text-align: center;margin-top: 2px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 15px;height: 15px;vertical-align: top;overflow: hidden;border-width: 3px;border-radius: 100%;border-style: solid;border-color: rgb(255, 255, 255) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);background-color: rgb(241, 203, 206);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: rotateZ(20deg);\" powered-by=\"xiumi.us\">\n<section style=\"\">\n<section style=\"display: inline-block;width: 20px;height: 20px;vertical-align: top;overflow: hidden;border-width: 1px;border-radius: 100%;border-style: solid;border-color: rgb(239, 135, 135);background-color: rgb(242, 203, 206);\">\n<section style=\"margin-top: 2px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 15px;height: 15px;vertical-align: top;overflow: hidden;border-width: 3px;border-radius: 100%;border-style: solid;border-color: rgb(255, 255, 255) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);background-color: rgb(241, 203, 206);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;background-color: rgb(255, 255, 255);overflow: hidden;padding-top: 46px;border-style: solid;border-width: 1px;border-color: rgb(241, 187, 186);\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;padding-right: 20px;padding-left: 20px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<ol class=\"list-paddingleft-1\" style=\"padding-left: 40px;list-style-position: outside;\">\n<li>\n<p>https:\/\/www.google.com.hk\/url?sa=i&amp;url=https%3A%2F%2Fcran.r-project.org%2Fweb%2Fpackages%2Fseqinr%2Fseqinr.pdf&amp;psig=AOvVaw0cuRmOBlRp2QJKn3CHhzwk&amp;ust=1689826667558000&amp;source=images&amp;cd=vfe&amp;opi=89978449&amp;ved=0CA4QjRxqFwoTCLj5oob1mYADFQAAAAAdAAAAABAH<br  \/><\/p>\n<\/li>\n<li>\n<p>https:\/\/www.biob.in\/2017\/10\/frequency-plot-of-protein-sequence.html<\/p>\n<\/li>\n<li>\n<p>https:\/\/www.google.com.hk\/url?sa=i&#038;url=https%3A%2F%2Fdavetang.org%2Fmuse%2F2013%2F05%2F09%2Fusing-the-r-seqinr-package%2F&#038;psig=AOvVaw2-KSI8UzLpWsVFIDlgS00M&#038;ust=1689827429884000&#038;source=images&#038;cd=vfe&#038;opi=89978449&#038;ved=0CA4QjRxqFwoTCLi5i_D3mYADFQAAAAAdAAAAABAQ<\/p>\n<\/li>\n<li>\n<p>https:\/\/www.google.com.hk\/url?sa=i&#038;url=http%3A%2F%2Fa-little-book-of-r-for-bioinformatics.readthedocs.io%2Fen%2Flatest%2Fsrc%2Fchapter2.html&#038;psig=AOvVaw2-KSI8UzLpWsVFIDlgS00M&#038;ust=1689827429884000&#038;source=images&#038;cd=vfe&#038;opi=89978449&#038;ved=0CA4QjRxqFwoTCLi5i_D3mYADFQAAAAAdAAAAABAg<\/p>\n<\/li>\n<li>\n<p>https:\/\/www.google.com.hk\/url?sa=i&#038;url=http%3A%2F%2Fa-little-book-of-r-for-bioinformatics.readthedocs.io%2Fen%2Flatest%2Fsrc%2Fchapter2_answers.html&#038;psig=AOvVaw2-KSI8UzLpWsVFIDlgS00M&#038;ust=1689827429884000&#038;source=images&#038;cd=vfe&#038;opi=89978449&#038;ved=0CA4QjRxqFwoTCLi5i_D3mYADFQAAAAAdAAAAABAo<\/p>\n<\/li>\n<\/ol>\n<\/section>\n<\/section>\n<\/section>\n<section 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