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14px;\">1.\u9996\u5148\u80af\u5b9a\u662f\u8981\u8f93\u5165\u6587\u4ef6\uff01\u5148\u6765\u6559\u6559\u5927\u5bb6h5ad\u6587\u4ef6\u7684\u8bfb\u53d6<\/span><\/strong><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">h5ad\u6587\u4ef6\u5168\u79f0\u662fh5anndata\uff0c\u662fanndata\u7684h5\u683c\u5f0f\uff0canndata\u4e5f\u662fpython\u4e2d\u7684\u6570\u636e\u683c\u5f0f\uff0c\u5982\u679c\u4f60\u60f3\u7528seurat\u8bfb\uff0c\u53ef\u4ee5\u53d1\u73b0\u6700\u540e\u8bfb\u53d6\u7684\u6587\u4ef6\u683c\u5f0f\u4f1a\u53d8\u4e3ah5seurat\uff0c\u7b80\u5355\u7684\u8bf4python\u4e2d\u9002\u914dh5ad\u683c\u5f0f\u8bfb\u53d6\uff0cR\u9002\u914dh5seurat\u683c\u5f0f\u8bfb\u53d6\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">adata =anndata.read_h5ad(‘F:\/university\/research\/\u7a7a\u95f4\u8f6c\u5f55\u7ec4\/canvas\/GSM6177599_10xVisium_processed.h5ad’)<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p><strong><span style=\"font-size: 14px;\">2.\u8bfb\u5165\u6587\u4ef6\u540e\uff0c\u8ba9\u6211\u4eec\u770b\u4e00\u4e0badata\u4e2d\u6709\u4ec0\u4e48<\/span><\/strong><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:\u5b8b\u4f53;font-size:12.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2111913357400722\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_2xFUWAhmaaMddaic6xUpErmVV3fDXA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyemnPRxOKYibE9nmkdEfQDbmPibwvXuMgtNicNf8uMvXrvR0TB2xFUWAhmaaMddaic6xUpErmVV3fDXA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">Obsm\uff1a\u5bf9\u4e8e\u89c2\u6d4b\u7684\u591a\u7ef4\u6ce8\u91ca\uff0c\u5b83\u662f\u53ef\u53d8\u7684ndarray\uff0c\u5c31\u662f\u8bf4\u7ef4\u5ea6\u662f\u53ef\u53d8\u7684\uff01\u5c0f\u679c\u5077\u5077\u544a\u8bc9\u4f60\uff0c\u8fd9\u91cc\u7684\u7ef4\u5ea6\u4e00\u822c\u662f2\u81f3\u591a\u7ef4\u54e6\u3002\u800cObsm\u8fd9\u91cc\u7684m\u6307\u7684\u5c31\u662fmulti-dim\u591a\u4e2a\u7ef4\u5ea6\u7684\uff0cobs_m\u5bf9\u5e94\u4e8eobs\uff0c\u4f46obs\u7684\u6bcf\u4e2a\u6210\u5458\u90fd\u662f\u4e00\u7ef4\u7684\u89c2\u6d4b\u6ce8\u91ca\uff0cobs_m\u7684\u6bcf\u4e2a\u6210\u5458\uff08X_pac\u548cX_umap\uff09\u90fd\u662f\u591a\u7ef4\u7684\u89c2\u6d4b\u6ce8\u91ca\u3002\u54e6\u5bf9\u4e86\uff0c\u5fd8\u4e86\u544a\u8bc9\u4f60\uff0cobs\u5c31\u662f\u77e9\u9635\u7684\u884c\uff0c\u4e5f\u5c31\u662f\u6837\u672c\uff01<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;\">Varm\uff1a<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\u4e0eobsm\u5bf9\u5e94\uff0c\u662f\u57fa\u56e0\u7684\u591a\u7ef4\u89c2\u6d4b~<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;\">uns\uff1a<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\u975e\u7ed3\u6784\u5316\u6570\u636e\uff0c\u4e5f\u5c31\u662f\u5b57\u5178\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;\">rank_genes_groups <\/span><\/strong><\/span><span style=\"font-size: 14px;\">\u8bc6\u522b\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\uff08\u6f5c\u5728\u7684marker\uff09\uff0c\u8fd9\u4e2a\u51fd\u6570\u5c06\u83b7\u53d6\u6bcf\u7ec4\u7ec6\u80de\uff0c\u5e76\u5c06\u6bcf\u7ec4\u4e2d\u6bcf\u4e2a\u57fa\u56e0\u7684\u5206\u5e03\u4e0e\u4e0d\u5728\u8be5\u7ec4\u4e2d\u7684\u6240\u6709\u5176\u4ed6\u7ec6\u80de\u4e2d\u7684\u5206\u5e03\u8fdb\u884c\u6bd4\u8f83\u6700\u540e\u8fdb\u884c\u8bc6\u522b\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;\">pca\uff1a<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\u4e3b\u6210\u5206\u5206\u6790\u964d\u4f4e\u6570\u636e\u7ef4\u5ea6\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;\">umap\u964d\u7ef4\uff1a<\/span><\/strong><\/span><span style=\"font-size: 14px;\">umap\u6bd4tsne\u4fdd\u7559\u5168\u5c40\u7ed3\u6784\uff0c\u5141\u8bb8\u5927\u6570\u636e\u8f93\u5165\uff0c\u8fd0\u884c\u65f6\u95f4\u77ed\uff0c\u8fd9\u662fumap\u548ctsne\u7684\u533a\u522b\uff0c\u4e00\u5b9a\u8981\u7262\u8bb0\u5594\uff01<\/span><o:p><\/o:p><\/p>\n<p><strong><span style=\"font-size: 14px;text-decoration: underline;\">obsp(pair)\uff1a<\/span><\/strong><span style=\"font-size: 14px;\">\u8868\u793a\u7ec6\u80de\u548c\u7ec6\u80de\u4e4b\u95f4\u7684\u8ddd\u79bb\u548c\u8fde\u901a\u6027\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">\u987a\u4fbf\u7ed9\u5927\u5bb6\u63d0\u63d0obs\u4e2d\u7684mito\u8868\u793a\u7ebf\u7c92\u4f53\u57fa\u56e0\uff0c\u4e00\u822cmito\u90fd\u8868\u793a\u7684\u662f\u7ebf\u7c92\u4f53\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;\">n_genes_by_counts`\uff1a<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\u6bcf\u4e2a\u7ec6\u80de\u4e2d\uff0c\u6709\u8868\u8fbe\u7684\u57fa\u56e0\u7684\u4e2a\u6570\uff1b<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;\">total_counts\uff1a<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\u6bcf\u4e2a\u7ec6\u80de\u7684\u57fa\u56e0\u603b\u8ba1\u6570\uff08\u603b\u8868\u8fbe\u91cf\uff09<\/span><o:p><\/o:p><\/p>\n<p><strong><span style=\"font-size: 14px;text-decoration: underline;\">pct_counts_mt\uff1a<\/span><\/strong><span style=\"font-size: 14px;\">\u6bcf\u4e2a\u7ec6\u80de\u4e2d\uff0c\u7ebf\u7c92\u4f53\u57fa\u56e0\u8868\u8fbe\u91cf\u5360\u8be5\u7ec6\u80de\u6240\u6709\u57fa\u56e0\u8868\u8fbe\u91cf\u7684\u767e\u5206\u6bd4<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">\u5148\u7ed9\u5927\u5bb6\u4ecb\u7ecd\u8fd9\u4e48\u591a\u4e86\uff0c\u5177\u4f53\u7684\u8fd8\u662f\u9700\u8981\u540e\u7eed\u81ea\u5df1\u67e5\u4e00\u4e0b\u54e6~<\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:\u5b8b\u4f53;font-size:12.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"text-decoration: none;\"><strong><span style=\"text-decoration: none;font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">3.\u53ef\u89c6\u5316\u4e00\u4e0b<\/span><\/strong><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"python\"><code><span class=\"code-snippet_outer\">import matplotlib.pyplot as plt<\/span><\/code><code><span class=\"code-snippet_outer\">from matplotlib import rc_context<\/span><\/code><code><span class=\"code-snippet_outer\"># rc_context\u7528\u4e8e\u6307\u5b9afigure\u5927\u5c0f<\/span><\/code><code><span class=\"code-snippet_outer\">with rc_context({'figure.figsize': (4, 4)}):<\/span><\/code><code><span class=\"code-snippet_outer\"># vmax\u6539\u53d8\u989c\u8272\u6700\u5927\u503c\uff0cframeon\u4e3a\u53bb\u6389\u8fb9\u6846<\/span><\/code><code><span class=\"code-snippet_outer\">sc.pl.umap(Myadata, color=['Rp1','clusters','pct_counts_mito','total_counts'],s=50,ncols=4,frameon=False,vmax='p99')<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2184115523465704\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_4cFZxEVMfgKGXyeYCqHqNQW0HUKaQQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyemnPRxOKYibE9nmkdEfQDb7uQrf8IaezickmQRibVVtuNqNm4cFZxEVMfgKGXyeYCqHqNQW0HUKaQQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e0a\u56fe\u4e2d\u6211\u4eec\u4f7f\u7528umap\u7ed8\u5236\u4e86\u5bf9\u5e94\u7684cluster\uff0cmetadata\u7684\u503c\uff0cmetadata\u5c31\u662f\u6bcf\u4e2a\u7ec6\u80de\u7684\u4fe1\u606f\u5594\uff0c\u8fd8\u7ed8\u5236\u4e86Rp1\uff0c\u8fd9\u4e48\u591a\u56fe\u53ea\u9700\u8981\u7b80\u5355\u7684\u4e24\u884c\u4ee3\u7801\u5c31\u53ef\u4ee5\u7ed8\u5236\uff01\u662f\u4e0d\u662f\u89c9\u5f97\u5199\u8fd9\u4e2a\u5305\u7684\u4eba\u5f88\u725b\uff01<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4f46\u662f\u5176\u5b9e\u5c0f\u679c\u8fd8\u662f\u60f3\u77e5\u9053\u8fd9\u4e2a\u5750\u6807\u662f\u54ea\u91cc\u6765\u7684\uff0c\u6240\u4ee5\u5c31\u53bb\u8bd5\u4e86\u4e00\u4e0badata\u4e2d\u7684obsm\u4e2d\u7684umap\u7684\u503c\uff0c\u60f3\u770b\u770b\u7a76\u7adf\u5728\u8fd9\u4e2a\u5305\u4e2d\u662f\u4e0d\u662fX_umap\u7684\u5750\u6807\u7ed8\u5236\u4e86\u4e0a\u9762\u8fd9\u6837\u7684\u56fe\uff0c\u4e8e\u662f\u5c31\u8fdb\u884c\u4e86\u4e0b\u8ff0\u64cd\u4f5c\uff1a<\/span><span style=\"mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:\u5b8b\u4f53;font-size:12.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"python\"><code><span class=\"code-snippet_outer\">#\u6d4b\u8bd5\u4e00\u4e0bumap \u7b26\u5408\u9884\u671f\uff0c\u786e\u5b9e\u662f\u6570\u636e\u4e3a\u7ec6\u80de\u7684\u5750\u6807<\/span><\/code><code><span class=\"code-snippet_outer\">import matplotlib.pyplot as plt<\/span><\/code><code><span class=\"code-snippet_outer\"># \u5c06\u4e8c\u7ef4\u6570\u7ec4\u8f6c\u6362\u4e3a\u4e24\u4e2a\u4e00\u7ef4\u6570\u7ec4x\u548cy<\/span><\/code><code><span class=\"code-snippet_outer\">data=Myadata.obsm['X_umap']<\/span><\/code><code><span class=\"code-snippet_outer\">x = [d[0] for d in data]<\/span><\/code><code><span class=\"code-snippet_outer\">y = [d[1] for d in data]<\/span><\/code><code><span class=\"code-snippet_outer\"># \u7ed8\u5236\u6563\u70b9\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\">plt.scatter(x, y<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.7429245283018868\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_ceXlmjy8zziaC0SO2bOMosRY2Sg7lw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyemnPRxOKYibE9nmkdEfQDbcF4lsPMSUyzLpzK2eA6V3fEEyiceXlmjy8zziaC0SO2bOMosRY2Sg7lw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"424\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u6211\u4eec\u53ef\u4ee5\u770b\u5230\uff0c\u786e\u5b9e\u662f\u8fd9\u6837\u7684\uff01\u6240\u4ee5\u5927\u5bb6\u4e4b\u540e\u8981\u662f\u4e0d\u61c2\u5305\u5185\u90e8\u7684\u539f\u7406\u7684\u8bdd\u4e00\u5b9a\u8981\u591a\u5c1d\u8bd5\uff0c\u591a\u8bd5\u8bd5\u603b\u5f52\u662f\u9a97\u4e0d\u4e86\u4eba\u7684\uff01<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"python\"><code><span class=\"code-snippet_outer\">with rc_context({'figure.figsize': (4.5, 3)}):<\/span><\/code><code><span class=\"code-snippet_outer\"> '''stripplot=False\u7528\u4e8e\u5220\u9664internal dots, inner='box'\u7528\u4e8e\u5728\u5c0f\u63d0\u7434\u5185\u90e8\u52a0\u65b9\u6846\u56fe'''<\/span><\/code><code><span class=\"code-snippet_outer\"> sc.pl.violin(Myadata, ['pct_counts_in_top_50_genes', 'total_counts_mito'], groupby='clusters', stripplot=False, inner='box')<\/span><\/code><code><span class=\"code-snippet_outer\"> '''mino\u8868\u793a\u7ebf\u7c92\u4f53\u57fa\u56e0'''<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u53ef\u4ee5\u518d\u6765\u8bd5\u8bd5\u5c0f\u63d0\u7434\u56fe\uff01<\/span><span style=\"mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:\u5b8b\u4f53;font-size:12.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3176895306859206\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_rHIpnia3hnBl29bePMY8ib7tb7TGFA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyemnPRxOKYibE9nmkdEfQDbeuYLIIHZpRY0AqQUdObFR2CYl8rHIpnia3hnBl29bePMY8ib7tb7TGFA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u8fd9\u662f11\u4e2acluster\u4e2d\u6bcf\u4e2acluster\u5bf9\u5e94\u7684\u4e0d\u540c\u7c7b\u7684\u57fa\u56e0\u7684\u5c0f\u63d0\u7434\u56fe\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 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AnnData(\u4e00\u79cd\u6570\u636e\u7ed3\u6784)\u8054\u5408\u6784\u5efa\u7684\u5355\u7ec6\u80de\u5206\u6790\u6570\u636e\uff0c\u5f53\u7136\u76ee\u524d\u968f\u7740\u6280\u672f\u7684\u53d1\u5c55\uff0c\u5b83\u7684\u5f00\u53d1\u8005\u4e5f\u5728\u8ddf\u968f\u65f6\u4ee3\u70ed\u70b9\u53d8\u5316\u4e0d\u65ad\u6539\u8fdb\u5b83\uff0c\u73b0\u5728\u9664\u4e86\u5355\u7ec6\u80de\u5206\u6790\uff0c\u5b83\u4e5f\u5b9e\u73b0\u4e86\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u6570\u636e\u5206\u6790\u6d41\u7a0b\u3002\u4e4b\u524d\u5c0f\u4f19\u4f34\u5728\u540e\u53f0\u95ee\u5c0f\u679cScanpy\u548cSeurat\u5305\u7684\u533a\u522b\uff0c\u8bf4\u81ea\u5df1\u4e0d\u662f\u5f88\u61c2\u5404\u79cd\u5206\u6790\u6d41\u7a0b\u7684\u6b65\u9aa4\u548c\u539f\u7406\uff0c\u679c\u679c\u4eca\u5929\u5c31\u7ed9\u5927\u5bb6\u8be6\u7ec6\u8bb2\u8bb2python\u7684scanpy\u5305\uff0c\u5176\u5b9escanpy\u548cseurat\u7684\u529f\u80fd\u57fa\u672c\u662f\u53ef\u4ee5\u66ff\u4ee3\u7684\uff0c\u4e0d\u540c\u7684\u662f\u4e00\u4e2a\u7528\u5728python,\u4e00\u4e2a\u7528\u5728R\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">\u6211\u4eec\u77e5\u9053\u5355\u7ec6\u80de\u548c\u7a7a\u8f6c\u5206\u6790\u6d41\u7a0b\u5305\u62ec\u5f88\u591a\u6b65\u9aa4\uff0c\u6bd4\u5982\u8d28\u63a7\uff08QC\uff09\uff0c\u6807\u51c6\u5316\uff08Normalization\uff09\uff0c\u964d\u7ef4\u805a\u7c7b\uff08Dimensional reduction and clustering\uff09\uff0cCluster marker genes\uff0c Spatially variable genes\u7b49\uff0c\u5c0f\u679c\u4eca\u5929\u5e76\u4e0d\u60f3\u76f4\u63a5\u7ed9\u5927\u5bb6\u4ecb\u7ecd\u7f51\u4e0a\u90fd\u6709\u7684\u6d41\u7a0b\uff0c\u800c\u662f\u60f3\u7ed9\u5927\u5bb6\u770b\u770bScanpy\u8bfb\u53d6\u7684\u6570\u636e\u7ed3\u6784\uff0c\u4ee5\u53ca\u5982\u4f55\u53ef\u89c6\u5316\uff0c\u6559\u5927\u5bb6\u4e00\u6b65\u5165\u95e8Scanpy!<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p><strong><span style=\"font-size: 14px;\">1.\u9996\u5148\u80af\u5b9a\u662f\u8981\u8f93\u5165\u6587\u4ef6\uff01\u5148\u6765\u6559\u6559\u5927\u5bb6h5ad\u6587\u4ef6\u7684\u8bfb\u53d6<\/span><\/strong><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">h5ad\u6587\u4ef6\u5168\u79f0\u662fh5anndata\uff0c\u662fanndata\u7684h5\u683c\u5f0f\uff0canndata\u4e5f\u662fpython\u4e2d\u7684\u6570\u636e\u683c\u5f0f\uff0c\u5982\u679c\u4f60\u60f3\u7528seurat\u8bfb\uff0c\u53ef\u4ee5\u53d1\u73b0\u6700\u540e\u8bfb\u53d6\u7684\u6587\u4ef6\u683c\u5f0f\u4f1a\u53d8\u4e3ah5seurat\uff0c\u7b80\u5355\u7684\u8bf4python\u4e2d\u9002\u914dh5ad\u683c\u5f0f\u8bfb\u53d6\uff0cR\u9002\u914dh5seurat\u683c\u5f0f\u8bfb\u53d6\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">adata =anndata.read_h5ad(‘F:\/university\/research\/\u7a7a\u95f4\u8f6c\u5f55\u7ec4\/canvas\/GSM6177599_10xVisium_processed.h5ad’)<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p><strong><span style=\"font-size: 14px;\">2.\u8bfb\u5165\u6587\u4ef6\u540e\uff0c\u8ba9\u6211\u4eec\u770b\u4e00\u4e0badata\u4e2d\u6709\u4ec0\u4e48<\/span><\/strong><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:\u5b8b\u4f53;font-size:12.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2111913357400722\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_2xFUWAhmaaMddaic6xUpErmVV3fDXA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyemnPRxOKYibE9nmkdEfQDbmPibwvXuMgtNicNf8uMvXrvR0TB2xFUWAhmaaMddaic6xUpErmVV3fDXA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">Obsm\uff1a\u5bf9\u4e8e\u89c2\u6d4b\u7684\u591a\u7ef4\u6ce8\u91ca\uff0c\u5b83\u662f\u53ef\u53d8\u7684ndarray\uff0c\u5c31\u662f\u8bf4\u7ef4\u5ea6\u662f\u53ef\u53d8\u7684\uff01\u5c0f\u679c\u5077\u5077\u544a\u8bc9\u4f60\uff0c\u8fd9\u91cc\u7684\u7ef4\u5ea6\u4e00\u822c\u662f2\u81f3\u591a\u7ef4\u54e6\u3002\u800cObsm\u8fd9\u91cc\u7684m\u6307\u7684\u5c31\u662fmulti-dim\u591a\u4e2a\u7ef4\u5ea6\u7684\uff0cobs_m\u5bf9\u5e94\u4e8eobs\uff0c\u4f46obs\u7684\u6bcf\u4e2a\u6210\u5458\u90fd\u662f\u4e00\u7ef4\u7684\u89c2\u6d4b\u6ce8\u91ca\uff0cobs_m\u7684\u6bcf\u4e2a\u6210\u5458\uff08X_pac\u548cX_umap\uff09\u90fd\u662f\u591a\u7ef4\u7684\u89c2\u6d4b\u6ce8\u91ca\u3002\u54e6\u5bf9\u4e86\uff0c\u5fd8\u4e86\u544a\u8bc9\u4f60\uff0cobs\u5c31\u662f\u77e9\u9635\u7684\u884c\uff0c\u4e5f\u5c31\u662f\u6837\u672c\uff01<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;\">Varm\uff1a<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\u4e0eobsm\u5bf9\u5e94\uff0c\u662f\u57fa\u56e0\u7684\u591a\u7ef4\u89c2\u6d4b~<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;\">uns\uff1a<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\u975e\u7ed3\u6784\u5316\u6570\u636e\uff0c\u4e5f\u5c31\u662f\u5b57\u5178\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;\">rank_genes_groups <\/span><\/strong><\/span><span style=\"font-size: 14px;\">\u8bc6\u522b\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\uff08\u6f5c\u5728\u7684marker\uff09\uff0c\u8fd9\u4e2a\u51fd\u6570\u5c06\u83b7\u53d6\u6bcf\u7ec4\u7ec6\u80de\uff0c\u5e76\u5c06\u6bcf\u7ec4\u4e2d\u6bcf\u4e2a\u57fa\u56e0\u7684\u5206\u5e03\u4e0e\u4e0d\u5728\u8be5\u7ec4\u4e2d\u7684\u6240\u6709\u5176\u4ed6\u7ec6\u80de\u4e2d\u7684\u5206\u5e03\u8fdb\u884c\u6bd4\u8f83\u6700\u540e\u8fdb\u884c\u8bc6\u522b\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;\">pca\uff1a<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\u4e3b\u6210\u5206\u5206\u6790\u964d\u4f4e\u6570\u636e\u7ef4\u5ea6\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;\">umap\u964d\u7ef4\uff1a<\/span><\/strong><\/span><span style=\"font-size: 14px;\">umap\u6bd4tsne\u4fdd\u7559\u5168\u5c40\u7ed3\u6784\uff0c\u5141\u8bb8\u5927\u6570\u636e\u8f93\u5165\uff0c\u8fd0\u884c\u65f6\u95f4\u77ed\uff0c\u8fd9\u662fumap\u548ctsne\u7684\u533a\u522b\uff0c\u4e00\u5b9a\u8981\u7262\u8bb0\u5594\uff01<\/span><o:p><\/o:p><\/p>\n<p><strong><span style=\"font-size: 14px;text-decoration: underline;\">obsp(pair)\uff1a<\/span><\/strong><span style=\"font-size: 14px;\">\u8868\u793a\u7ec6\u80de\u548c\u7ec6\u80de\u4e4b\u95f4\u7684\u8ddd\u79bb\u548c\u8fde\u901a\u6027\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">\u987a\u4fbf\u7ed9\u5927\u5bb6\u63d0\u63d0obs\u4e2d\u7684mito\u8868\u793a\u7ebf\u7c92\u4f53\u57fa\u56e0\uff0c\u4e00\u822cmito\u90fd\u8868\u793a\u7684\u662f\u7ebf\u7c92\u4f53\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;\">n_genes_by_counts`\uff1a<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\u6bcf\u4e2a\u7ec6\u80de\u4e2d\uff0c\u6709\u8868\u8fbe\u7684\u57fa\u56e0\u7684\u4e2a\u6570\uff1b<\/span><o:p><\/o:p><\/p>\n<p><span style=\"text-decoration: underline;\"><strong><span style=\"text-decoration: underline;font-size: 14px;\">total_counts\uff1a<\/span><\/strong><\/span><span style=\"font-size: 14px;\">\u6bcf\u4e2a\u7ec6\u80de\u7684\u57fa\u56e0\u603b\u8ba1\u6570\uff08\u603b\u8868\u8fbe\u91cf\uff09<\/span><o:p><\/o:p><\/p>\n<p><strong><span style=\"font-size: 14px;text-decoration: underline;\">pct_counts_mt\uff1a<\/span><\/strong><span style=\"font-size: 14px;\">\u6bcf\u4e2a\u7ec6\u80de\u4e2d\uff0c\u7ebf\u7c92\u4f53\u57fa\u56e0\u8868\u8fbe\u91cf\u5360\u8be5\u7ec6\u80de\u6240\u6709\u57fa\u56e0\u8868\u8fbe\u91cf\u7684\u767e\u5206\u6bd4<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">\u5148\u7ed9\u5927\u5bb6\u4ecb\u7ecd\u8fd9\u4e48\u591a\u4e86\uff0c\u5177\u4f53\u7684\u8fd8\u662f\u9700\u8981\u540e\u7eed\u81ea\u5df1\u67e5\u4e00\u4e0b\u54e6~<\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:\u5b8b\u4f53;font-size:12.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"text-decoration: none;\"><strong><span style=\"text-decoration: none;font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">3.\u53ef\u89c6\u5316\u4e00\u4e0b<\/span><\/strong><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"python\"><code><span class=\"code-snippet_outer\">import matplotlib.pyplot as plt<\/span><\/code><code><span class=\"code-snippet_outer\">from matplotlib import rc_context<\/span><\/code><code><span class=\"code-snippet_outer\"># rc_context\u7528\u4e8e\u6307\u5b9afigure\u5927\u5c0f<\/span><\/code><code><span class=\"code-snippet_outer\">with rc_context({'figure.figsize': (4, 4)}):<\/span><\/code><code><span class=\"code-snippet_outer\"># vmax\u6539\u53d8\u989c\u8272\u6700\u5927\u503c\uff0cframeon\u4e3a\u53bb\u6389\u8fb9\u6846<\/span><\/code><code><span class=\"code-snippet_outer\">sc.pl.umap(Myadata, color=['Rp1','clusters','pct_counts_mito','total_counts'],s=50,ncols=4,frameon=False,vmax='p99')<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2184115523465704\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_4cFZxEVMfgKGXyeYCqHqNQW0HUKaQQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyemnPRxOKYibE9nmkdEfQDb7uQrf8IaezickmQRibVVtuNqNm4cFZxEVMfgKGXyeYCqHqNQW0HUKaQQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e0a\u56fe\u4e2d\u6211\u4eec\u4f7f\u7528umap\u7ed8\u5236\u4e86\u5bf9\u5e94\u7684cluster\uff0cmetadata\u7684\u503c\uff0cmetadata\u5c31\u662f\u6bcf\u4e2a\u7ec6\u80de\u7684\u4fe1\u606f\u5594\uff0c\u8fd8\u7ed8\u5236\u4e86Rp1\uff0c\u8fd9\u4e48\u591a\u56fe\u53ea\u9700\u8981\u7b80\u5355\u7684\u4e24\u884c\u4ee3\u7801\u5c31\u53ef\u4ee5\u7ed8\u5236\uff01\u662f\u4e0d\u662f\u89c9\u5f97\u5199\u8fd9\u4e2a\u5305\u7684\u4eba\u5f88\u725b\uff01<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4f46\u662f\u5176\u5b9e\u5c0f\u679c\u8fd8\u662f\u60f3\u77e5\u9053\u8fd9\u4e2a\u5750\u6807\u662f\u54ea\u91cc\u6765\u7684\uff0c\u6240\u4ee5\u5c31\u53bb\u8bd5\u4e86\u4e00\u4e0badata\u4e2d\u7684obsm\u4e2d\u7684umap\u7684\u503c\uff0c\u60f3\u770b\u770b\u7a76\u7adf\u5728\u8fd9\u4e2a\u5305\u4e2d\u662f\u4e0d\u662fX_umap\u7684\u5750\u6807\u7ed8\u5236\u4e86\u4e0a\u9762\u8fd9\u6837\u7684\u56fe\uff0c\u4e8e\u662f\u5c31\u8fdb\u884c\u4e86\u4e0b\u8ff0\u64cd\u4f5c\uff1a<\/span><span style=\"mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:\u5b8b\u4f53;font-size:12.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"python\"><code><span class=\"code-snippet_outer\">#\u6d4b\u8bd5\u4e00\u4e0bumap \u7b26\u5408\u9884\u671f\uff0c\u786e\u5b9e\u662f\u6570\u636e\u4e3a\u7ec6\u80de\u7684\u5750\u6807<\/span><\/code><code><span class=\"code-snippet_outer\">import matplotlib.pyplot as plt<\/span><\/code><code><span class=\"code-snippet_outer\"># \u5c06\u4e8c\u7ef4\u6570\u7ec4\u8f6c\u6362\u4e3a\u4e24\u4e2a\u4e00\u7ef4\u6570\u7ec4x\u548cy<\/span><\/code><code><span class=\"code-snippet_outer\">data=Myadata.obsm['X_umap']<\/span><\/code><code><span class=\"code-snippet_outer\">x = [d[0] for d in data]<\/span><\/code><code><span class=\"code-snippet_outer\">y = [d[1] for d in data]<\/span><\/code><code><span class=\"code-snippet_outer\"># \u7ed8\u5236\u6563\u70b9\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\">plt.scatter(x, y<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.7429245283018868\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_ceXlmjy8zziaC0SO2bOMosRY2Sg7lw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyemnPRxOKYibE9nmkdEfQDbcF4lsPMSUyzLpzK2eA6V3fEEyiceXlmjy8zziaC0SO2bOMosRY2Sg7lw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"424\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u6211\u4eec\u53ef\u4ee5\u770b\u5230\uff0c\u786e\u5b9e\u662f\u8fd9\u6837\u7684\uff01\u6240\u4ee5\u5927\u5bb6\u4e4b\u540e\u8981\u662f\u4e0d\u61c2\u5305\u5185\u90e8\u7684\u539f\u7406\u7684\u8bdd\u4e00\u5b9a\u8981\u591a\u5c1d\u8bd5\uff0c\u591a\u8bd5\u8bd5\u603b\u5f52\u662f\u9a97\u4e0d\u4e86\u4eba\u7684\uff01<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"python\"><code><span class=\"code-snippet_outer\">with rc_context({'figure.figsize': (4.5, 3)}):<\/span><\/code><code><span class=\"code-snippet_outer\"> '''stripplot=False\u7528\u4e8e\u5220\u9664internal dots, inner='box'\u7528\u4e8e\u5728\u5c0f\u63d0\u7434\u5185\u90e8\u52a0\u65b9\u6846\u56fe'''<\/span><\/code><code><span class=\"code-snippet_outer\"> sc.pl.violin(Myadata, ['pct_counts_in_top_50_genes', 'total_counts_mito'], groupby='clusters', stripplot=False, inner='box')<\/span><\/code><code><span class=\"code-snippet_outer\"> '''mino\u8868\u793a\u7ebf\u7c92\u4f53\u57fa\u56e0'''<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u53ef\u4ee5\u518d\u6765\u8bd5\u8bd5\u5c0f\u63d0\u7434\u56fe\uff01<\/span><span style=\"mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:\u5b8b\u4f53;font-size:12.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3176895306859206\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_rHIpnia3hnBl29bePMY8ib7tb7TGFA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyemnPRxOKYibE9nmkdEfQDbeuYLIIHZpRY0AqQUdObFR2CYl8rHIpnia3hnBl29bePMY8ib7tb7TGFA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u8fd9\u662f11\u4e2acluster\u4e2d\u6bcf\u4e2acluster\u5bf9\u5e94\u7684\u4e0d\u540c\u7c7b\u7684\u57fa\u56e0\u7684\u5c0f\u63d0\u7434\u56fe\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u603b\u7ed3\u4e00\u4e0b\u53ed\uff0cpl.umap\u53ef\u4ee5\u753bumap\u964d\u7ef4\u56fe\uff0cpl.violin\u53ef\u4ee5\u753b\u5c0f\u63d0\u7434\u56fe\uff0c\u90a3\u4f60\u77e5\u9053\u70ed\u56fe\u600e\u4e48\u753b\u5417\uff0c\u8fd8\u6709\u5806\u53e0\u5c0f\u63d0\u7434\u56fe\uff0c\u6bd4\u5982\u4e0b\u9762\u8fd9\u79cd\u56fe\u6240\u793a\uff0c\u662f\u4e0d\u662f\u5f88\u597d\u770b\u54c8\u54c8\u54c8\uff0c\u5c0f\u4f19\u4f34\u4eec\u53ef\u4ee5\u4e4b\u540e\u8bd5\u8bd5\u54e6\uff0c\u5982\u679c\u6709\u9700\u8981\u7684\u8bdd\uff0c\u5c0f\u679c\u4e5f\u4f1a\u4e13\u95e8\u7ed9\u5927\u5bb6\u51fa\u4e00\u671f\u7ee7\u7eed\u8bb2\u4e00\u4e0b\u6211\u4eec\u8fd9\u6b21\u6240\u7528\u6570\u636e\u7684\u540e\u7eed\u5206\u6790\u7684\uff0c\u5c31\u770b\u5927\u5bb6\u7684\u8d5e\u7684\u4e2a\u6570\u4e86\u54e6\u3002<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.8461538461538461\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_z16SF0FF1Mic0hOHecicXqHrcRA0zA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyemnPRxOKYibE9nmkdEfQDbvYaUhAAr11atCGJcY0Uicr29Ug3z16SF0FF1Mic0hOHecicXqHrcRA0zA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"299\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:\u5b8b\u4f53;font-size:12.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u597d\u5566~\u4eca\u5929\u5c0f\u679c\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\u5566\uff01\u5982\u679c\u5c0f\u4f19\u4f34\u6709\u5176\u4ed6\u6570\u636e\u5206\u6790\u9700\u6c42\uff0c\u53ef\u4ee5\u5c1d\u8bd5\u4f7f\u7528\u672c\u516c\u53f8\u65b0\u5f00\u53d1\u7684\u751f\u4fe1\u5206\u6790\u5c0f\u5de5\u5177\u4e91\u5e73\u53f0\uff0c\u96f6\u4ee3\u7801\u5b8c\u6210\u5206\u6790\uff0c\u975e\u5e38\u65b9\u4fbf\u5965\uff0c\u4e91\u5e73\u53f0\u7f51\u5740\u4e3a\uff1a(<\/span><span style=\"color: rgb(5, 99, 193);text-decoration: underline;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">http:\/\/www.biocloudservice.com\/home.html<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">)\uff0c\u5176\u4e2d\u4e5f\u5305\u62ec\u4e86\u901a\u8def\u8868\u8fbe\u5206\u6790(<\/span><span style=\"color: rgb(5, 99, 193);text-decoration: underline;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">http:\/\/www.biocloudservice.com\/313\/313.php<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">)\uff0c\u5355\u7ec6\u80de\u7684\u57fa\u56e0\u5171\u8868\u8fbe\u5206\u6790(<\/span><span style=\"color: rgb(5, 99, 193);text-decoration: underline;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">http:\/\/www.biocloudservice.com\/906\/906.php<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">)\u7b49\u5404\u79cd\u5c0f\u5de5\u5177\u54e6~\uff0c\u6709\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u767b\u5f55\u7f51\u7ad9\u8fdb\u884c\u4e86\u89e3\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"color: rgb(255, 0, 0);font-size: 10.5pt;background: rgb(255, 255, 255);font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u4e91\u751f\u4fe1\u5e73\u53f0\u94fe\u63a5\uff1a<\/span><span style=\"text-decoration: underline;font-family: \u5fae\u8f6f\u96c5\u9ed1;color: rgb(255, 0, 0);background: rgb(255, 255, 255);\">http:\/\/www.biocloudservice.com\/home.html\u3002<\/span><span style=\"mso-spacerun:"yes";font-family:\u5fae\u8f6f\u96c5\u9ed1;color:rgb(255,0,0);font-size:10.5000pt;mso-font-kerning:1.0000pt;background:rgb(255,255,255);mso-shading:rgb(255,255,255);\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:"yes";font-family:\u5fae\u8f6f\u96c5\u9ed1;color:rgb(255,0,0);font-size:10.5000pt;mso-font-kerning:1.0000pt;background:rgb(255,255,255);mso-shading:rgb(255,255,255);\">\u4e91\u751f\u4fe1\u5e73\u53f0\u94fe\u63a5\uff1ahttp:\/\/www.biocloudservice.com\/home.html\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0px;outline: 0px;white-space: normal;\"><span style=\"outline: 0px;font-size: 14px;\"> <\/span><span style=\"outline: 0px;font-size: var(--articleFontsize);letter-spacing: 0.034em;\"><\/span><\/p>\n<section data-mpa-powered-by=\"yiban.io\" style=\"margin-bottom: 0px;outline: 0px;letter-spacing: 0.578px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section data-role=\"list\" style=\"outline: 0px;visibility: visible;\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"padding-right: 15px;padding-left: 15px;outline: 0px;font-size: 14px;\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;height: auto;transform: perspective(0px);transform-style: flat;\">\n<section style=\"margin-top: 10px;margin-bottom: -3px;outline: 0px;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;transform: translate3d(19px, 0px, 0px) rotateX(180deg);\">\n<section style=\"outline: 0px;display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: center;\">\n<section style=\"outline: 0px;display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 230px;background-color: rgb(217, 217, 217);\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: justify;\">\n<p style=\"outline: 0px;\"><br style=\"outline: 0px;\" \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"outline: 0px;display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section 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nowrap;\">\n<section style=\"padding-left: 9px;outline: 0px;display: inline-block;width: 625px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 0px 12px;border-left-color: rgb(212, 32, 2);\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: justify;font-size: 17px;color: rgb(62, 62, 62);\">\n<p style=\"outline: 0px;\"><strong style=\"outline: 0px;\">\u5c0f\u679c\u53cb\u60c5\u63a8\u8350<\/strong><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 3px;outline: 0px;\">\n<section style=\"outline: 0px;background-color: rgb(217, 217, 217);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: justify;font-size: 11px;color: rgb(62, 62, 62);\">\n<p style=\"outline: 0px;\">\u597d\u7528\u53c8\u514d\u8d39\u7684\u5de5\u5177\u5b89\u5229<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;outline: 0px;text-align: center;line-height: 0;\">\n<section style=\"outline: 0px;vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.35777777777777775\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_eVibso6VJox4Hnic0n53VnLS1A9qYA-1.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAyqVJd7As6iaIgTzXqErqUGpoEpMoWhEkVHHw4z3ZmlYpZZUX7eVibso6VJox4Hnic0n53VnLS1A9qYA\/640?wx_fmt=jpeg&wxfrom=5&wx_lazy=1&wx_co=1'\" data-type=\"jpeg\" data-w=\"900\" style=\"outline: 0px;vertical-align: middle;visibility: visible !important;width: 625px !important;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;outline: 0px;text-align: center;line-height: 0;\">\n<section style=\"outline: 0px;vertical-align: middle;display: inline-block;line-height: 0;\"><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650762044&idx=2&sn=c74f81e91cadd166adc7168dec9891ed&scene=21#wechat_redirect\" textvalue=\"\u4f60\u5df2\u9009\u4e2d\u4e86\u6dfb\u52a0\u94fe\u63a5\u7684\u5185\u5bb9\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\" style=\"outline: 0px;vertical-align: bottom;user-select: none;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.35361653272101035\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_UwKXVVrT2bVTc2jSlZNaUIE5TUCDMQ-1.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAyqVJd7As6iaIgTzXqErqUGp8QxUSk0Sricia0JSmh8nB9T4SicUwKXVVrT2bVTc2jSlZNaUIE5TUCDMQ\/640?wx_fmt=jpeg&wxfrom=5&wx_lazy=1&wx_co=1'\" data-type=\"jpeg\" data-w=\"871\" style=\"outline: 0px;border-width: 0px;border-style: initial;border-color: initial;vertical-align: middle;visibility: visible !important;width: 625px !important;\" data-recalc-dims=\"1\"><\/span><\/a><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;outline: 0px;text-align: center;line-height: 0;\">\n<section style=\"outline: 0px;vertical-align: middle;display: inline-block;line-height: 0;\"><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650761303&idx=2&sn=6f73471d1e82c56c0b2160d0fcd32d53&scene=21#wechat_redirect\" textvalue=\"\u4f60\u5df2\u9009\u4e2d\u4e86\u6dfb\u52a0\u94fe\u63a5\u7684\u5185\u5bb9\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\" style=\"outline: 0px;vertical-align: bottom;user-select: none;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.36333333333333334\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_IHSYtLyxwVWxiaf2v2rQdOnoz05G6A-1.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAyqVJd7As6iaIgTzXqErqUGp9ZZibzJmVhqwxDqOgC1Tdmh7VxIHSYtLyxwVWxiaf2v2rQdOnoz05G6A\/640?wx_fmt=jpeg&wxfrom=5&wx_lazy=1&wx_co=1'\" data-type=\"jpeg\" data-w=\"900\" style=\"outline: 0px;border-width: 0px;border-style: initial;border-color: initial;vertical-align: middle;visibility: visible !important;width: 625px !important;\" data-recalc-dims=\"1\"><\/span><\/a><\/section>\n<p><span style=\"outline: 0px;text-align: justify;letter-spacing: 0.5px;text-indent: 0em;color: rgb(0, 150, 136);font-size: 32px;caret-color: rgba(0, 0, 0, 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