{"id":55138,"date":"2024-06-16T16:15:55","date_gmt":"2024-06-16T08:15:55","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55138"},"modified":"2024-06-16T16:15:55","modified_gmt":"2024-06-16T08:15:55","slug":"%e7%94%a8r%e8%bf%9b%e8%a1%8cgwas%e5%88%86%e6%9e%90%ef%bc%8c%e5%8e%9f%e6%9d%a5%e5%a6%82%e6%ad%a4%e7%ae%80%e5%8d%95","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55138","title":{"rendered":"\u7528R\u8fdb\u884cGWAS\u5206\u6790\uff0c\u539f\u6765\u5982\u6b64\u7b80\u5355"},"content":{"rendered":"


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style=\"display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(249, 172, 172);background-color: rgb(249, 172, 172);width: 1.8em;height: 1.8em;line-height: 1.8em;border-radius: 100%;margin-left: auto;margin-right: auto;font-size: 17px;color: rgb(255, 255, 255);\">\n<p>\u70b9<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"font-size: 19px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(249, 172, 172);background-color: rgb(249, 172, 172);width: 1.8em;height: 1.8em;line-height: 1.8em;border-radius: 100%;margin-left: auto;margin-right: auto;font-size: 17px;color: rgb(255, 255, 255);\">\n<p>\u51fb<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"font-size: 19px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(249, 172, 172);background-color: rgb(249, 172, 172);width: 1.8em;height: 1.8em;line-height: 1.8em;border-radius: 100%;margin-left: auto;margin-right: auto;font-size: 17px;color: rgb(255, 255, 255);\">\n<p>\u84dd<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"font-size: 19px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(249, 172, 172);background-color: rgb(249, 172, 172);width: 1.8em;height: 1.8em;line-height: 1.8em;border-radius: 100%;margin-left: auto;margin-right: auto;font-size: 17px;color: rgb(255, 255, 255);\">\n<p>\u5b57<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"color: rgb(255, 226, 93);font-size: 12px;\" powered-by=\"xiumi.us\">\n<p>\u2605<\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"font-size: 19px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(249, 172, 172);background-color: rgb(249, 172, 172);width: 1.8em;height: 1.8em;line-height: 1.8em;border-radius: 100%;margin-left: auto;margin-right: auto;font-size: 17px;color: rgb(255, 255, 255);\">\n<p>\u5173<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"font-size: 19px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(249, 172, 172);background-color: rgb(249, 172, 172);width: 1.8em;height: 1.8em;line-height: 1.8em;border-radius: 100%;margin-left: auto;margin-right: auto;font-size: 17px;color: rgb(255, 255, 255);\">\n<p>\u6ce8<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"font-size: 19px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(249, 172, 172);background-color: rgb(249, 172, 172);width: 1.8em;height: 1.8em;line-height: 1.8em;border-radius: 100%;margin-left: auto;margin-right: auto;font-size: 17px;color: rgb(255, 255, 255);\">\n<p>\u6211<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"font-size: 19px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(249, 172, 172);background-color: rgb(249, 172, 172);width: 1.8em;height: 1.8em;line-height: 1.8em;border-radius: 100%;margin-left: auto;margin-right: auto;font-size: 17px;color: rgb(255, 255, 255);\">\n<p>\u4eec<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;white-space: normal;\">\u4f7f\u7528R\u8bed\u8a00\u505aGWAS\u5206\u6790\uff0c\u6709\u4e9b\u4eba\u5bf9R\u8bed\u8a00\u53ef\u80fd\u6bd4\u8f83\u719f\u6089\uff0c\u5c0f\u5e08\u59b9\u8fd9\u91cc\u63d0\u4f9b\u4e86\u4e00\u4e2a\u7528R\u8bed\u8a00\u5206\u6790GWAS\u7684\u6d41\u7a0b\u3002\u8be5\u6d41\u7a0b\u6709\uff1aGWAS\u7684QC\uff0cPCA\u5206\u6790\uff0c\u9009\u62e9\u4e0d\u540c\u6a21\u578bGWAS\u5206\u6790\u3002<\/p>\n<p style=\"text-indent: 0em;white-space: normal;\"><br \/><\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">\u5168\u57fa\u56e0\u7ec4\u5173\u8054\u5206\u6790\uff08Genome wide association study\uff0cGWAS\uff09\u662f\u5bf9\u591a\u4e2a\u4e2a\u4f53\u5728\u5168\u57fa\u56e0\u7ec4\u8303\u56f4\u7684\u9057\u4f20\u53d8\u5f02\uff08\u6807\u8bb0\uff09\u591a\u6001\u6027\u8fdb\u884c\u68c0\u6d4b\uff0c\u83b7\u5f97\u57fa\u56e0\u578b\uff0c\u8fdb\u800c\u5c06\u57fa\u56e0\u578b\u4e0e\u53ef\u89c2\u6d4b\u7684\u6027\u72b6\uff0c\u5373\u8868\u578b\uff0c\u8fdb\u884c\u7fa4\u4f53\u6c34\u5e73\u7684\u7edf\u8ba1\u5b66\u5206\u6790\uff0c\u6839\u636e\u7edf\u8ba1\u91cf\u6216\u663e\u8457\u6027P\u503c\u7b5b\u9009\u51fa\u6700\u6709\u53ef\u80fd\u5f71\u54cd\u8be5\u6027\u72b6\u7684\u9057\u4f20\u53d8\u5f02\uff08\u6807\u8bb0\uff09\uff0c\u6316\u6398\u4e0e\u6027\u72b6\u53d8\u5f02\u76f8\u5173\u7684\u57fa\u56e0\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 20px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;border-style: solid;border-width: 1px;min-width: 5%;height: auto;box-shadow: rgb(239, 135, 135) 6px 6px 0px 0px;padding: 8px;\">\n<section style=\"text-align: left;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: justify;font-size: 17px;\">\n<p style=\"white-space: normal;\"><strong>GWAS\u5206\u6790\u6a21\u578b\u4ecb\u7ecd<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 0em;white-space: normal;\" powered-by=\"xiumi.us\">GWAS \u5206\u6790\u4e00\u822c\u4f1a\u6784\u5efa\u56de\u5f52\u6a21\u578b\u68c0\u9a8c\u6807\u8bb0\u4e0e\u8868\u578b\u4e4b\u95f4\u662f\u5426\u5b58\u5728\u5173\u8054\u3002GWAS\u4e2d\u7684\u96f6\u5047\u8bbe\uff08H0 null hypothesis\uff09\u662f\u6807\u8bb0\u7684\u56de\u5f52\u7cfb\u6570\u4e3a\u96f6, \u6807\u8bb0\u5bf9\u8868\u578b\u6ca1\u6709\u5f71\u54cd\u3002\u5907\u62e9\u5047\u8bbe(H1\uff0c\u4e5f\u53eb\u5bf9\u7acb\u5047\u8bbe\uff0cAlternative Hypothesis)\u662f\u6807\u8bb0\u7684\u56de\u5f52\u7cfb\u6570\u4e0d\u4e3a\u96f6\uff0cSNP\u548c\u8868\u578b\u76f8\u5173\u3002GWAS\u4e2d\u7684\u6a21\u578b\u4e3b\u8981\u5206\u4e3a\u4e24\u79cd\uff1a<\/p>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"color: rgb(249, 110, 87);\"><strong>\u4e00\u822c\u7ebf\u6027\u6a21\u578bGLM(General Linear Model)\uff1a<\/strong><\/span>y = X\u03b2 + Z\u03b1 + e<\/p>\n<p style=\"white-space: normal;\"><span style=\"color: rgb(249, 110, 87);\"><strong>\u6df7\u5408\u7ebf\u6027\u6a21\u578bMLM(Mixed Linear Model)\uff1a<\/strong><\/span>y = X\u03b2 + Z\u03b1 + W\u03bc+ e<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong>y: <\/strong>\u6240\u8981\u7814\u7a76\u7684\u8868\u578b\u6027\u72b6\uff1b<\/p>\n<p style=\"white-space: normal;\"><strong>X\u03b2<\/strong>\uff1a\u56fa\u5b9a\u6548\u5e94\uff08Fixed Effect\uff09\uff0c\u5f71\u54cdy\u7684\u5176\u4ed6\u56e0\u7d20\uff0c\u4e3b\u8981\u6307\u7fa4\u4f53\u7ed3\u6784\uff1b<\/p>\n<p style=\"white-space: normal;\"><strong>Z\u03b1\uff1a<\/strong>\u6807\u8bb0\u6548\u5e94\uff08Marker Effect  SNP\uff09\uff1b<\/p>\n<p style=\"white-space: normal;\"><strong>W\u03bc\uff1a<\/strong>\u968f\u673a\u6548\u5e94\uff08RandomEffect\uff09\uff0c\u8fd9\u91cc\u4e00\u822c\u6307\u4e2a\u4f53\u7684\u4eb2\u7f18\u5173\u7cfb\u3002<\/p>\n<p style=\"white-space: normal;\"><strong>e: <\/strong>\u6b8b\u5dee<\/p>\n<\/section>\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 20px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;border-style: solid;border-width: 1px;min-width: 5%;height: auto;box-shadow: rgb(239, 135, 135) 6px 6px 0px 0px;padding: 8px;\">\n<section style=\"text-align: left;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: justify;font-size: 17px;\">\n<p style=\"white-space: normal;\"><strong>GWAS\u6a21\u578b\u8be6\u7ec6\u4ecb\u7ecd<\/strong><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u4e00\u822c\u7ebf\u6027\u6a21\u578bGLM\uff1a\u76f4\u63a5\u5c06\u57fa\u56e0\u578bx\u548c\u8868\u578by\u505a\u56de\u5f52\u62df\u5408\u3002\u4e5f\u53ef\u4ee5\u52a0\u5165\u7fa4\u4f53\u7ed3\u679c\u63a7\u5236\u5047\u9633\u6027\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3210862619808307\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_OlE1gDMSADb07nIgRH1eKtd7ect6sw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEUJBKoTRbNLxokvrcju55fH0dg29RrNa4VFOgIIwFJR8WxMOlE1gDMSADb07nIgRH1eKtd7ect6sw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"626\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><span style=\"color: rgb(249, 110, 87);\"><strong>\u6df7\u5408\u7ebf\u6027\u6a21\u578bMLM\uff1a<\/strong><\/span>GLM\u6a21\u578b\u4e2d\uff0c\u5982\u679c\u4e24\u4e2a\u8868\u578b\u5dee\u5f02\u5f88\u5927\uff0c\u4f46\u7fa4\u4f53\u672c\u8eab\u8fd8\u542b\u6709\u5176\u4ed6\u7684\u9057\u4f20\u5dee\u5f02\uff08\u5982\u5730\u57df\u7b49\uff09\uff0c\u5219\u90a3\u4e9b\u4e0e\u8be5\u8868\u578b\u65e0\u5173\u7684\u9057\u4f20\u5dee\u5f02\u4e5f\u4f1a\u5f71\u54cd\u5230\u76f8\u5173\u6027\u3002MLM\u6a21\u578b\u53ef\u4ee5\u628a\u7fa4\u4f53\u7ed3\u6784\u7684\u5f71\u54cd\u8bbe\u4e3a\u534f\u65b9\u5dee\uff0c\u628a\u8fd9\u79cd\u4f4d\u70b9\u6821\u6b63\u6389\u3002\u6b64\u5916\uff0c\u6750\u6599\u95f4\u7684\u516c\u5171\u7956\u5148\u5173\u7cfb\u4e5f\u4f1a\u5bfc\u81f4\u975e\u8fde\u9501\u76f8\u5173\uff0c\u53ef\u52a0\u5165\u4eb2\u7f18\u5173\u7cfb\u77e9\u9635\u4f5c\u4e3a\u968f\u673a\u6548\u5e94\u6765\u77eb\u6b63\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.18055555555555555\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_iaVRRRT8dqTacwt2KdnSgG4a1b9hhA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEUJBKoTRbNLxokvrcju55fHfCUytvmw0Db1FIiccpbTpgrhPbiaVRRRT8dqTacwt2KdnSgG4a1b9hhA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"576\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"color: rgb(249, 110, 87);\"><strong>FarmCPU\uff1a<\/strong><\/span>GWAS\u7684\u74f6\u9888\u4e00\u662f\u8ba1\u7b97\u901f\u5ea6\uff0c\u4e8c\u662f\u7edf\u8ba1\u51c6\u786e\u6027\u3002FarmCPU\u80fd\u63d0\u5347\u901f\u5ea6\u548c\u51c6\u786e\u6027\uff0c\u9996\u5148\u628a\u968f\u673a\u6548\u5e94\u7684\u4eb2\u7f18\u5173\u7cfb\u77e9\u9635\uff08Kinship\uff09\u8f6c\u6362\u4e3a\u56fa\u5b9a\u6548\u5e94\u7684\u5173\u8054SNP\u77e9\u9635\uff08S\u77e9\u9635\/QTNs\u77e9\u9635\uff09\uff0c\u4f7f\u8ba1\u7b97\u901f\u5ea6\u5927\u5927\u52a0\u5feb\uff1b\u518d\u5229\u7528QTN\u77e9\u9635\u5f53\u505a\u534f\u53d8\u91cf\uff0c\u91cd\u65b0\u505a\u5173\u8054\u5206\u6790\uff0c\u63d0\u5347\u51c6\u786e\u7387\u3002<br \/><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\">\u8bdd\u4e0d\u591a\u8bf4\u76f4\u63a5\u4e0a\u4ee3\u7801<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># get current working directory<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cur_wd<-getwd()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># set path of working directory<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">setwd(cur_wd)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># quality control for gene chip<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">system(<span class=\"code-snippet__string\">'plink  --cow  --file 1-data-holstein  --mind 0.1  --geno 0.1  --maf 0.05  --hwe 0.00001  --recode  --out 2-data-holstein_qc'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">system(<span class=\"code-snippet__string\">'plink  --cow  --file 1-data-holstein  --mind 0.1  --geno 0.1  --maf 0.05  --hwe 0.00001  --make-bed  --out 2-data-holstein_qc'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">system(<span class=\"code-snippet__string\">'plink  --cow  --file 1-data-holstein  --mind 0.1  --geno 0.1  --maf 0.05  --hwe 0.00001  --recodeA  --out 2-data-holstein_qc'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#PCA<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">system(<span class=\"code-snippet__string\">'plink  --cow  --bfile 2-data-holstein_qc  --pca 20  --out 2-data-holstein_pca'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># plot for PCA<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># import data of eigenvectors and eigenvalues<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pca_eigenvectors<-read.table(<span class=\"code-snippet__string\">'2-data-holstein_pca.eigenvec'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">pca_eigenval<-read.table(<span class=\"code-snippet__string\">'2-data-holstein_pca.eigenval'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#calculating percentage of eigenvalues<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pca_eigenval$percentage<-pca_eigenval$V1\/<span class=\"code-snippet__built_in\">sum<\/span>(pca_eigenval$V1)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#plot<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pca_value1<-paste(<span class=\"code-snippet__string\">\"PCA1(\"<\/span>,<span class=\"code-snippet__number\">100<\/span>*<span class=\"code-snippet__built_in\">round<\/span>(pca_eigenval$percentage[<span class=\"code-snippet__number\">1<\/span>],<span class=\"code-snippet__number\">3<\/span>),<span class=\"code-snippet__string\">\"%)\"<\/span>,sep = <span class=\"code-snippet__string\">\"\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">pca_value2<-paste(<span class=\"code-snippet__string\">\"PCA2(\"<\/span>,<span class=\"code-snippet__number\">100<\/span>*<span class=\"code-snippet__built_in\">round<\/span>(pca_eigenval$percentage[<span class=\"code-snippet__number\">2<\/span>],<span class=\"code-snippet__number\">3<\/span>),<span class=\"code-snippet__string\">\"%)\"<\/span>,sep = <span class=\"code-snippet__string\">\"\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># output plot<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">tiff(<span class=\"code-snippet__string\">\"2-plot-PCA.tiff\"<\/span>, width = <span class=\"code-snippet__number\">1000<\/span>,height=<span class=\"code-snippet__number\">1000<\/span>,res = <span class=\"code-snippet__number\">100<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">par(mar=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__number\">5<\/span>,<span class=\"code-snippet__number\">5<\/span>,<span class=\"code-snippet__number\">5<\/span>,<span class=\"code-snippet__number\">5<\/span>),cex.axis=<span class=\"code-snippet__number\">2<\/span>,cex.lab=<span class=\"code-snippet__number\">2<\/span>, cex.axis=<span class=\"code-snippet__number\">2<\/span>, lwd=<span class=\"code-snippet__number\">2<\/span> ,bty=<span class=\"code-snippet__string\">\"l\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">plot(pca_eigenvectors[,<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__number\">3<\/span>,<span class=\"code-snippet__number\">4<\/span>)],pch=<span class=\"code-snippet__number\">16<\/span>,col=rainbow(<span class=\"code-snippet__number\">100<\/span>),xlab=pca_value1,ylab=pca_value2)<\/span><\/code><code><span class=\"code-snippet_outer\">dev.off()<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.0344827586206897\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_q3evH22my9uCrCUiaG3HaTCosVfY5w.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEUJBKoTRbNLxokvrcju55fHw3iawhwyAUJm8n3qHIXjfzcaibgq3evH22my9uCrCUiaG3HaTCosVfY5w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"957\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># export eigenvectors as covariate<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">write.table(pca_eigenvectors[,<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">7<\/span>],<span class=\"code-snippet__string\">\"2-data-covariate_pca.txt\"<\/span>,row.names = <span class=\"code-snippet__built_in\">F<\/span>,col.names = <span class=\"code-snippet__built_in\">F<\/span>,<span class=\"code-snippet__built_in\">quote<\/span> = <span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># t test<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">system(<span class=\"code-snippet__string\">\"plink --cow --bfile 2-data-holstein_qc --pheno 1-data-phenotype.txt --mpheno 1 --linear --out 3-result-1-ttest\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># glm<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">system(<span class=\"code-snippet__string\">\"plink --cow --bfile 2-data-holstein_qc --pheno 1-data-phenotype.txt --mpheno 1 --covar 2-data-covariate_pca.txt --covar-number 1-5 --linear --out 3-result-2-glm\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># mlm<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">system(<span class=\"code-snippet__string\">\"gcta64 --autosome-num 30 --bfile 2-data-holstein_qc --pheno 1-data-phenotype.txt --mpheno 1 --mlma --out 3-result-3-mlm\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># mlm Q+K<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">system(<span class=\"code-snippet__string\">\"gcta64 --autosome-num 30 --bfile 2-data-holstein_qc --pheno 1-data-phenotype.txt --mpheno 1 --qcovar 2-data-covariate_pca.txt --mlma --out 3-result-4-mlm\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#FarmCPU<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"bigmemory\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"biganalytics\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">library(bigmemory)<\/span><\/code><code><span class=\"code-snippet_outer\">library(biganalytics)<\/span><\/code><code><span class=\"code-snippet_outer\">library(compiler)<\/span><\/code><code><span class=\"code-snippet_outer\">source(<span class=\"code-snippet__string\">'1-farmcpu.txt'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">source(<span class=\"code-snippet__string\">'1-gapit.txt'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#import phenotype data<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">phenotype<-read.table(<span class=\"code-snippet__string\">\"1-data-phenotype.txt\"<\/span>,header = <span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">phenotype<-phenotype[,-<span class=\"code-snippet__number\">1<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">names<\/span>(phenotype)<-<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"id\"<\/span>,<span class=\"code-snippet__string\">\"yield\"<\/span>,<span class=\"code-snippet__string\">\"fat\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#import map for genotype(3 column):SNPChromosomePosition<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">genotype_map<-read.table(<span class=\"code-snippet__string\">\"2-data-holstein_qc.map\"<\/span>,header = <span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">genotype_map<-genotype_map[,<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__number\">2<\/span>,<span class=\"code-snippet__number\">1<\/span>,<span class=\"code-snippet__number\">4<\/span>)]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">names<\/span>(genotype_map)<-<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"SNP\"<\/span>,<span class=\"code-snippet__string\">\"Chromosome\"<\/span>,<span class=\"code-snippet__string\">\"Position\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#import genotype:ID+genotype(numeric:0 1 2),titile=T:ID+snp_name<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">genotype<-read.big.matrix(<span class=\"code-snippet__string\">\"2-data-holstein_qc.raw\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">type=<span class=\"code-snippet__string\">\"char\"<\/span>,sep = <span class=\"code-snippet__string\">\" \"<\/span>,head = <span class=\"code-snippet__literal\">TRUE<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">genotype<-genotype[,-<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__number\">1<\/span>,<span class=\"code-snippet__number\">3<\/span>:<span class=\"code-snippet__number\">6<\/span>)]<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#set the threshold for first iteration step#<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">first_thero=<span class=\"code-snippet__built_in\">max<\/span>(<span class=\"code-snippet__number\">0.05<\/span>\/table(genotype_map[,<span class=\"code-snippet__number\">2<\/span>]))<\/span><\/code><code><span class=\"code-snippet_outer\">myFarmCPU<-FarmCPU(<\/span><\/code><code><span class=\"code-snippet_outer\">Y=phenotype[,<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__number\">1<\/span>,<span class=\"code-snippet__number\">2<\/span>)],<\/span><\/code><code><span class=\"code-snippet_outer\">GD=genotype,<\/span><\/code><code><span class=\"code-snippet_outer\">GM=genotype_map,<\/span><\/code><code><span class=\"code-snippet_outer\">p.threshold = first_thero,<\/span><\/code><code><span class=\"code-snippet_outer\">cutOff = <span class=\"code-snippet__number\">0.05<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">MAF.calculate = <span class=\"code-snippet__built_in\">F<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">threshold.output = <span class=\"code-snippet__number\">1<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#method.bin = \"optimum\",<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">DPP=<span class=\"code-snippet__number\">10000<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># import results<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># t test<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">result_ttest<-read.table(<span class=\"code-snippet__string\">\"3-result-1-ttest.assoc.linear\"<\/span>,header = <span class=\"code-snippet__built_in\">T<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3527777777777778\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_XB6EZZx3fhFV9cmTAZDeicjfI1JLwQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEUJBKoTRbNLxokvrcju55fH7NAAA9eKI1ibNSoEZFBjC7og6fXB6EZZx3fhFV9cmTAZDeicjfI1JLwQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># glm<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">result_glm<-read.table(<span class=\"code-snippet__string\">\"3-result-2-glm.assoc.linear\"<\/span>,header = <span class=\"code-snippet__built_in\">T<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">result_glm<-result_glm[which(result_glm$TEST==<span class=\"code-snippet__string\">\"ADD\"<\/span>),]<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.35\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_vMYkZzpoQ9L2W0Bgyg46icicPDX7VA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEUJBKoTRbNLxokvrcju55fH981SP5sox39Z2c5PYyQH8MExicdvMYkZzpoQ9L2W0Bgyg46icicPDX7VA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#mlm<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">result_mlm<-read.table(<span class=\"code-snippet__string\">\"3-result-3-mlm.mlma\"<\/span>,header = <span class=\"code-snippet__built_in\">T<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.41203703703703703\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_S8npbu4VgqAdKBOXLsL8jE3Iv3WCxQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEUJBKoTRbNLxokvrcju55fHx32iap4wyqE8xdGvby4NgpDhmS8npbu4VgqAdKBOXLsL8jE3Iv3WCxQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#mlm Q+K<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">result_mlm_q<-read.table(<span class=\"code-snippet__string\">\"3-result-4-mlm.mlma\"<\/span>,header = <span class=\"code-snippet__built_in\">T<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.42407407407407405\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_gpQelZwb1QmHeL4WbSWpU4z8jibpzw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEUJBKoTRbNLxokvrcju55fHlvVUNgUqFYwmjzl2ZQnwGxMRhgpQelZwb1QmHeL4WbSWpU4z8jibpzw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># farmcpu<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">result_farmcpu<-read.csv(paste(<span class=\"code-snippet__string\">\"FarmCPU\"<\/span>,<span class=\"code-snippet__built_in\">names<\/span>(phenotype)[<span class=\"code-snippet__number\">2<\/span>],<span class=\"code-snippet__string\">\"GWAS\"<\/span>,<span class=\"code-snippet__string\">\"Results.csv\"<\/span>,sep = <span class=\"code-snippet__string\">\".\"<\/span>),header = <span class=\"code-snippet__built_in\">T<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: 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powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: 33.33%;align-self: center;flex: 0 0 auto;\">\n<section style=\"margin-top: 0.5em;margin-bottom: 0.5em;\" powered-by=\"xiumi.us\">\n<section style=\"border-top: 1px dashed rgb(249, 172, 172);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;margin-right: 20px;margin-left: 20px;\">\n<section style=\"margin-top: -3px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: justify;color: rgb(246, 207, 63);font-size: 20px;\">\n<p style=\"text-align: center;white-space: normal;\">\u2605<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 33.3333%;align-self: center;flex: 0 0 auto;\">\n<section style=\"margin-top: 0.5em;margin-bottom: 0.5em;\" powered-by=\"xiumi.us\">\n<section style=\"border-top: 1px dashed rgb(249, 172, 172);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-align: center;margin-bottom: 24px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5555555555555556\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_akXKU5NcO1KwuWc1Rk7ZJ56LC2pYrQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEXjjmsEC5aXz0RE4be2ib1fJPnBD8yX0Z6OF2zocd2ngHpLOakXKU5NcO1KwuWc1Rk7ZJ56LC2pYrQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"900\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;font-size: 16px;letter-spacing: 0.578px;white-space: normal;outline: 0px;visibility: visible;\">\n<p style=\"outline: 0px;text-indent: 0em;text-align: center;\"><span style=\"outline: 0px;color: rgb(61, 170, 214);\"><strong style=\"outline: 0px;\">\u540d\u989d\u6709\u9650\uff0c\u8d76\u5feb\u626b\u7801\u8054\u7cfb\u5c0f\u5e08\u59b9\u5427\uff01<\/strong><\/span><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-top: -9px;margin-bottom: -9px;font-size: 16px;letter-spacing: 0.578px;white-space: normal;outline: 0px;transform: scale(0.8);transform-origin: center center;\">\n<section style=\"margin-top: 10px;outline: 0px;text-align: center;\">\n<section style=\"outline: 0px;display: inline-block;width: 230px;height: 90px;vertical-align: top;overflow: hidden;background-position: 50% 50%;background-repeat: no-repeat;background-size: contain;background-attachment: scroll;background-image: url("https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/RpJ9AibwAxrG8iamwKic8QRJJIpZMqzC02xOQw0C8ibibRibsA1rQBt3icOicvKyd1rEhUJ53W15gOdV4TTF30uWoDOPbQ\/640?wx_fmt=png");\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 25px;outline: 0px;\">\n<section style=\"outline: 0px;font-size: 18px;\">\n<p style=\"outline: 0px;\"><strong style=\"outline: 0px;\">\u6b22\u8fce\u6765\u64a9(*\uffe3\u2207\uffe3*)<br style=\"outline: 0px;\" \/><\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 10px;font-size: 16px;letter-spacing: 0.578px;white-space: normal;outline: 0px;text-align: center;\">\n<section style=\"outline: 0px;vertical-align: middle;display: inline-block;line-height: 0;width: 202.222px;height: auto;border-style: solid;border-width: 1px;border-color: rgb(62, 62, 62);\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"200\" data-cropsely1=\"0\" data-cropsely2=\"200\" data-ratio=\"1.0018484288354899\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_I52OGm24eW45FCK2cgDIqSzZhAfVWA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEXRNlIw1HzXsIJnb07XINMcknmicibjrXk3Lyv9L3icCJSicu8eI52OGm24eW45FCK2cgDIqSzZhAfVWA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"541\" style=\"vertical-align: middle;outline: 0px;width: 200px;visibility: visible !important;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7528R\u8fdb\u884cGWAS\u5206\u6790\uff0c\u539f\u6765\u5982\u6b64\u7b80\u5355 \u5c0f\u5e08\u59b9  \u751f\u4fe1\u679c  2023-09 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