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rgb(249, 172, 172);box-shadow: rgb(249, 172, 172) 0px 0px 5px 3px;\">\n<section style=\"text-align: center;transform: translate3d(2px, 0px, 0px);margin-top: -14px;margin-bottom: -14px;\" powered-by=\"xiumi.us\">\n<p>{ \u70b9\u51fb\u84dd\u5b57\uff0c\u5173\u6ce8\u6211\u4eec }<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u5728RNA-seq\u5b9e\u9a8c\u4e2d\uff0c\u6211\u4eec\u901a\u5e38\u4f1a\u83b7\u5f97\u5927\u91cf\u7684\u57fa\u56e0\u8868\u8fbe\u6570\u636e\uff0c\u6bcf\u4e2a\u6837\u672c\u90fd\u5305\u542b\u6570\u5343\u4e2a\u57fa\u56e0\u7684\u8868\u8fbe\u91cf\u3002\u8fd9\u4e9b\u6570\u636e\u975e\u5e38\u590d\u6742\uff0c\u96be\u4ee5\u76f4\u63a5\u5206\u6790\u548c\u7406\u89e3\u3002\u800cPCA\uff08Principal Component Analysis\uff09\u4e3b\u6210\u5206\u5206\u6790\u53ef\u4ee5\u5c06\u8fd9\u4e9b\u590d\u6742\u7684\u6570\u636e\u964d\u7ef4\u5230\u8f83\u4f4e\u7684\u7ef4\u5ea6\uff0c\u5e76\u53ef\u89c6\u5316\u6210\u4e8c\u7ef4\u6216\u4e09\u7ef4\u56fe\u5f62\uff0c\u4ece\u800c\u66f4\u597d\u5730\u7406\u89e3\u6570\u636e\u7684\u7ed3\u6784\u548c\u6a21\u5f0f\u3002<br \/><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\">PCA\u7684\u6838\u5fc3\u662f\u9009\u62e9\u65b0\u7684\u5750\u6807\u7cfb\uff08\u4e3b\u6210\u5206\uff09\uff0c\u5750\u6807\u8f74\u5782\u76f4\uff08\u4e5f\u5c31\u662f\u6b63\u4ea4\uff0c\u7ebf\u6027\u65e0\u5173\uff09\uff0c\u65b9\u5dee\u8db3\u591f\u5927\uff08\u4e3a\u4e86\u4f7f\u6570\u636e\u5728\u5750\u6807\u8f74\u7684\u6295\u5c04\u70b9\u5c3d\u53ef\u80fd\u7684\u5e7f\uff0c\u663e\u793a\u51fa\u66f4\u591a\u6570\u636e\u7279\u5f81\uff09<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong>\u5728RNA-seq\u4e2d\uff0cPCA\u53ef\u4ee5\u7528\u4e8e\u4ee5\u4e0b\u51e0\u4e2a\u65b9\u9762\uff1a<\/strong><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong><span style=\"color: rgb(249, 110, 87);\">\u6570\u636e\u964d\u7ef4<\/span><\/strong>\uff1a\u901a\u8fc7PCA\u53ef\u4ee5\u5c06\u9ad8\u7ef4\u7684RNA-seq\u6570\u636e\u964d\u7ef4\u5230\u8f83\u4f4e\u7684\u7ef4\u5ea6\uff0c\u540c\u65f6\u4fdd\u7559\u6570\u636e\u7684\u5927\u90e8\u5206\u53d8\u5f02\u6027\u3002\u8fd9\u6837\u53ef\u4ee5\u51cf\u5c11\u6570\u636e\u7684\u566a\u58f0\u548c\u5197\u4f59\u4fe1\u606f\uff0c\u4ece\u800c\u66f4\u597d\u5730\u5c55\u73b0\u6570\u636e\u7684\u4e3b\u8981\u7279\u5f81\u3002<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong><span style=\"color: rgb(249, 110, 87);\">\u53ef\u89c6\u5316<\/span><\/strong>\uff1a\u901a\u8fc7PCA\u53ef\u4ee5\u5c06RNA-seq\u6570\u636e\u53ef\u89c6\u5316\u4e3a\u4e8c\u7ef4\u6216\u4e09\u7ef4\u56fe\u5f62\uff0c\u4ece\u800c\u66f4\u597d\u5730\u7406\u89e3\u6570\u636e\u7684\u7ed3\u6784\u548c\u6a21\u5f0f\u3002\u4f8b\u5982\uff0c\u6211\u4eec\u53ef\u4ee5\u5c06\u4e0d\u540c\u6837\u672c\u4e4b\u95f4\u7684\u57fa\u56e0\u8868\u8fbe\u91cf\u7ed8\u5236\u6210\u4e00\u4e2a\u6563\u70b9\u56fe\uff0c\u4e0d\u540c\u6837\u672c\u7684\u8868\u8fbe\u6a21\u5f0f\u5c31\u53ef\u4ee5\u5728\u56fe\u5f62\u4e2d\u5448\u73b0\u51fa\u6765\u3002<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong><span style=\"color: rgb(249, 110, 87);\">\u6570\u636e\u63a2\u7d22<\/span><\/strong>\uff1a\u901a\u8fc7PCA\u53ef\u4ee5\u53d1\u73b0RNA-seq\u6570\u636e\u7684\u76f8\u5173\u6027\u548c\u5dee\u5f02\u6027\uff0c\u5e2e\u52a9\u6211\u4eec\u53d1\u73b0\u4e0d\u540c\u6837\u672c\u4e4b\u95f4\u7684\u5dee\u5f02\u548c\u5171\u540c\u70b9\u3002\u4f8b\u5982\uff0c\u6211\u4eec\u53ef\u4ee5\u5206\u6790PCA\u56fe\u4e2d\u7684\u6bcf\u4e2a\u4e3b\u6210\u5206\uff0c\u627e\u51fa\u5bf9\u5dee\u5f02\u6027\u6700\u5927\u7684\u57fa\u56e0\uff0c\u8fdb\u4e00\u6b65\u7814\u7a76\u8fd9\u4e9b\u57fa\u56e0\u7684\u751f\u7269\u5b66\u529f\u80fd\u548c\u76f8\u5173\u75be\u75c5\u3002<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\">\u6211\u4eec\u968f\u673a\u751f\u6210\u4e24\u7ec4\u6a21\u62df\u57fa\u56e0\u6570\u636e\uff0c\u5e76\u8fdb\u884cPCA\u964d\u7ef4\u3002<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">set.seed(<span class=\"code-snippet__number\">123<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">n <- 100<\/span><\/code><code><span class=\"code-snippet_outer\">mean_A <- 5<\/span><\/code><code><span class=\"code-snippet_outer\">sd_A <- 2<\/span><\/code><code><span class=\"code-snippet_outer\">mean_B <- 8<\/span><\/code><code><span class=\"code-snippet_outer\">sd_B <- 2<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">gene_data_A <- matrix(rnorm(n * <span class=\"code-snippet__number\">5<\/span>, mean = mean_A, sd = sd_A), nrow = <span class=\"code-snippet__number\">50<\/span>, ncol = <span class=\"code-snippet__number\">10<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">gene_data_B <- matrix(rnorm(n * <span class=\"code-snippet__number\">5<\/span>, mean = mean_B, sd = sd_B), nrow = <span class=\"code-snippet__number\">50<\/span>, ncol = <span class=\"code-snippet__number\">10<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">gene_data <- rbind(gene_data_A, gene_data_B)<\/span><\/code><code><span class=\"code-snippet_outer\">colnames(gene_data) <- paste0(<span class=\"code-snippet__string\">\"Gene\"<\/span>, <span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">10<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">rownames(gene_data) <- <span class=\"code-snippet__number\">1<\/span>:nrow(gene_data)<\/span><\/code><code><span class=\"code-snippet_outer\">group <- data.frame(Sample = <span class=\"code-snippet__number\">1<\/span>:nrow(gene_data), Group = <span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"GroupA\"<\/span>, <span class=\"code-snippet__string\">\"GroupB\"<\/span>), each = <span class=\"code-snippet__number\">50<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u53ef\u4ee5\u76f4\u63a5\u4f7f\u7528FactoMineR \u5305\u8c03\u7528PCA\u3002FactoMineR\u662f\u4e00\u4e2a\u7528\u4e8e\u591a\u5143\u63a2\u7d22\u6027\u6570\u636e\u5206\u6790\u548c\u6570\u636e\u6316\u6398\u7684R\u5305\u3002\u5b83\u4e3a\u591a\u7ef4\u6570\u636e\u7684\u964d\u7ef4\u3001\u805a\u7c7b\u548c\u53ef\u89c6\u5316\u63d0\u4f9b\u4e86\u5e7f\u6cdb\u7684\u65b9\u6cd5\u3002<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">gene.pca <- PCA(gene_data, ncp = <span class=\"code-snippet__number\">2<\/span>, scale.unit = <span class=\"code-snippet__literal\">TRUE<\/span>, graph = <span class=\"code-snippet__literal\">FALSE<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u4f7f\u7528PCA\u964d\u7ef4\uff0c\u63d0\u53d6\u8d21\u732e\u5ea6\u6700\u9ad8\u7684\u4e24\u4e2a\u7279\u5f81<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u63d0\u53d6\u6837\u672c\u5728 PCA \u524d\u4e24\u8f74\u4e2d\u7684\u5750\u6807<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pca_sample <- data.frame(gene.pca$ind$coord[ ,<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">2<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\">pca_sample$Sample=row.names(pca_sample)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u63d0\u53d6 PCA \u524d\u4e24\u8f74\u7684\u8d21\u732e\u5ea6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pca_eig1 <- <span class=\"code-snippet__built_in\">round<\/span>(gene.pca$eig[<span class=\"code-snippet__number\">1<\/span>,<span class=\"code-snippet__number\">2<\/span>], <span class=\"code-snippet__number\">2<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">pca_eig2 <- <span class=\"code-snippet__built_in\">round<\/span>(gene.pca$eig[<span class=\"code-snippet__number\">2<\/span>,<span class=\"code-snippet__number\">2<\/span>],<span class=\"code-snippet__number\">2<\/span> )<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u5bf9\u964d\u7ef4\u7ed3\u679c\u8fdb\u884c\u53ef\u89c6\u5316<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">pca_sample <- merge(pca_sample, group,by=<span class=\"code-snippet__string\">\"Sample\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">head(pca_sample)<\/span><\/code><code><span class=\"code-snippet_outer\">p <- ggplot(data = pca_sample, aes(x = Dim.1, y = Dim.2)) +<\/span><\/code><code><span class=\"code-snippet_outer\">geom_point(aes(color = Group), size = <span class=\"code-snippet__number\">2<\/span>) + <span class=\"code-snippet__comment\">#\u6839\u636e\u6837\u672c\u5750\u6807\u7ed8\u5236\u4e8c\u7ef4\u6563\u70b9\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">scale_color_manual(values = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">'orange'<\/span>, <span class=\"code-snippet__string\">'purple'<\/span>)) + <span class=\"code-snippet__comment\">#\u81ea\u5b9a\u4e49\u989c\u8272<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">theme(panel.grid = element_blank(), panel.background = element_rect(color = <span class=\"code-snippet__string\">'black'<\/span>, fill = <span class=\"code-snippet__string\">'transparent'<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">legend.key = element_rect(fill = <span class=\"code-snippet__string\">'transparent'<\/span>)) + <span class=\"code-snippet__comment\">#\u53bb\u9664\u80cc\u666f\u548c\u7f51\u683c\u7ebf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">labs(x = paste(<span class=\"code-snippet__string\">'PCA1:'<\/span>, pca_eig1, <span class=\"code-snippet__string\">'%'<\/span>), y = paste(<span class=\"code-snippet__string\">'PCA2:'<\/span>, pca_eig2, <span class=\"code-snippet__string\">'%'<\/span>), color = <span class=\"code-snippet__string\">''<\/span>) <span class=\"code-snippet__comment\">#\u5c06 PCA \u8f74\u8d21\u732e\u5ea6\u6dfb\u52a0\u5230\u5750\u6807\u8f74\u6807\u9898\u4e2d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">p<\/span><\/code><code><span class=\"code-snippet_outer\">p + stat_ellipse(aes(color = Group), level = <span class=\"code-snippet__number\">0.95<\/span>, show.legend = <span class=\"code-snippet__literal\">FALSE<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">p + stat_ellipse(aes(fill = Group), geom = <span class=\"code-snippet__string\">'polygon'<\/span>, level = <span class=\"code-snippet__number\">0.95<\/span>, alpha = <span class=\"code-snippet__number\">0.3<\/span>, show.legend = <span class=\"code-snippet__literal\">FALSE<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">scale_fill_manual(values = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">'orange'<\/span>, <span class=\"code-snippet__string\">'purple'<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7045929018789144\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_z8eAFtm0qH9oj5FKnqicVOKdXCibSg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEWaOVp4oK5ibDkBelm3NzicicQLtFC0YNLByA2c2WqnOcUveZiaNicz8eAFtm0qH9oj5FKnqicVOKdXCibSg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"958\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u8fd9\u5c31\u662f\u4f7f\u7528R\u5bf9\u591a\u7ef4\u7684\u57fa\u56e0\u6570\u636e\u8fdb\u884cPCA\u7684\u8fc7\u7a0b\u5566\uff0c\u662f\u4e0d\u662f\u975e\u5e38\u7b80\u5355\uff01\u5feb\u548c\u5c0f\u5e08\u59b9\u4e00\u8d77\u8bd5\u8bd5\u5427\uff01<\/p>\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;height: auto;align-self: center;margin-right: 10px;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(249, 172, 172);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;border-style: solid;border-width: 1px;border-color: rgb(249, 172, 172);padding-right: 8px;padding-left: 8px;margin-right: 10px;\">\n<section style=\"text-align: justify;color: rgb(249, 172, 172);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">E<\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;border-style: solid;border-width: 1px;border-color: rgb(249, 172, 172);padding-right: 8px;padding-left: 8px;margin-right: 10px;\">\n<section style=\"text-align: justify;color: rgb(249, 172, 172);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">N<\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;border-style: solid;border-width: 1px;border-color: rgb(249, 172, 172);padding-right: 8px;padding-left: 8px;margin-right: 10px;\">\n<section style=\"text-align: justify;color: rgb(249, 172, 172);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">D<\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 100 100 0%;height: auto;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(249, 172, 172);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-align: center;margin-bottom: 24px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5555555555555556\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_icWAmgGMVEzREAJmM59ov44ziaMCGg-5.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/oCSOtP61aEWjRWAeR9wicY9JyrK13cNBaR2PPYHOsBTrS4rJOUOnJCEIxt1icWAmgGMVEzREAJmM59ov44ziaMCGg\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"900\" style=\"\" \/><\/p>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u57fa\u56e0\u7ef4\u5ea6\u592a\u9ad8\u600e\u4e48\u529e\uff1f\u65e0\u6240\u8c13\uff01PCA\u964d\u7ef4\u4f1a\u51fa\u624b\uff01 \u5c0f\u5e08\u59b9 \u751f\u4fe1\u679c 2 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55181"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=55181"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55181\/revisions"}],"predecessor-version":[{"id":55184,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55181\/revisions\/55184"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=55181"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=55181"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=55181"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

{"id":55181,"date":"2024-06-16T16:18:00","date_gmt":"2024-06-16T08:18:00","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55181"},"modified":"2024-06-16T16:18:00","modified_gmt":"2024-06-16T08:18:00","slug":"%e5%9f%ba%e5%9b%a0%e7%bb%b4%e5%ba%a6%e5%a4%aa%e9%ab%98%e6%80%8e%e4%b9%88%e5%8a%9e%ef%bc%9f%e6%97%a0%e6%89%80%e8%b0%93%ef%bc%81pca%e9%99%8d%e7%bb%b4%e4%bc%9a%e5%87%ba%e6%89%8b%ef%bc%81","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55181","title":{"rendered":"\u57fa\u56e0\u7ef4\u5ea6\u592a\u9ad8\u600e\u4e48\u529e\uff1f\u65e0\u6240\u8c13\uff01PCA\u964d\u7ef4\u4f1a\u51fa\u624b\uff01"},"content":{"rendered":"

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\n<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 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Mono\",Menlo,Courier,monospace}.code-snippet__comment,.code-snippet__quote{color:#afafaf;font-style:italic}.code-snippet__keyword,.code-snippet__selector-tag,.code-snippet__subst{color:#ca7d37}.code-snippet__number,.code-snippet__literal,.code-snippet__variable,.code-snippet__template-variable,.code-snippet__tag .code-snippet__attr{color:#0e9ce5}.code-snippet__string,.code-snippet__doctag{color:#d14}.code-snippet__title,.code-snippet__section,.code-snippet__selector-id{color:#d14}.code-snippet__subst{font-weight:normal}.code-snippet__type,.code-snippet__class .code-snippet__title{color:#0e9ce5}.code-snippet__tag,.code-snippet__name,.code-snippet__attribute{color:#0e9ce5;font-weight:normal}.code-snippet__regexp,.code-snippet__link{color:#ca7d37}.code-snippet__symbol,.code-snippet__bullet{color:#d14}.code-snippet__built_in,.code-snippet__builtin-name{color:#ca7d37}.code-snippet__meta{color:#afafaf}.code-snippet__deletion{background:#fdd}.code-snippet__addition{background:#dfd}.code-snippet__emphasis{font-style:italic}.code-snippet__strong{font-weight:bold}.account_avatar{width:40px;height:40px;padding:0;}.account_info{display:-webkit-box;display:-webkit-flex;display:flex;-webkit-box-align:center;-webkit-align-items:center;padding:20px 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rgb(249, 172, 172);box-shadow: rgb(249, 172, 172) 0px 0px 5px 3px;\">\n<section style=\"text-align: center;transform: translate3d(2px, 0px, 0px);margin-top: -14px;margin-bottom: -14px;\" powered-by=\"xiumi.us\">\n<p>{ \u70b9\u51fb\u84dd\u5b57\uff0c\u5173\u6ce8\u6211\u4eec }<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u5728RNA-seq\u5b9e\u9a8c\u4e2d\uff0c\u6211\u4eec\u901a\u5e38\u4f1a\u83b7\u5f97\u5927\u91cf\u7684\u57fa\u56e0\u8868\u8fbe\u6570\u636e\uff0c\u6bcf\u4e2a\u6837\u672c\u90fd\u5305\u542b\u6570\u5343\u4e2a\u57fa\u56e0\u7684\u8868\u8fbe\u91cf\u3002\u8fd9\u4e9b\u6570\u636e\u975e\u5e38\u590d\u6742\uff0c\u96be\u4ee5\u76f4\u63a5\u5206\u6790\u548c\u7406\u89e3\u3002\u800cPCA\uff08Principal Component Analysis\uff09\u4e3b\u6210\u5206\u5206\u6790\u53ef\u4ee5\u5c06\u8fd9\u4e9b\u590d\u6742\u7684\u6570\u636e\u964d\u7ef4\u5230\u8f83\u4f4e\u7684\u7ef4\u5ea6\uff0c\u5e76\u53ef\u89c6\u5316\u6210\u4e8c\u7ef4\u6216\u4e09\u7ef4\u56fe\u5f62\uff0c\u4ece\u800c\u66f4\u597d\u5730\u7406\u89e3\u6570\u636e\u7684\u7ed3\u6784\u548c\u6a21\u5f0f\u3002<br \/><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\">PCA\u7684\u6838\u5fc3\u662f\u9009\u62e9\u65b0\u7684\u5750\u6807\u7cfb\uff08\u4e3b\u6210\u5206\uff09\uff0c\u5750\u6807\u8f74\u5782\u76f4\uff08\u4e5f\u5c31\u662f\u6b63\u4ea4\uff0c\u7ebf\u6027\u65e0\u5173\uff09\uff0c\u65b9\u5dee\u8db3\u591f\u5927\uff08\u4e3a\u4e86\u4f7f\u6570\u636e\u5728\u5750\u6807\u8f74\u7684\u6295\u5c04\u70b9\u5c3d\u53ef\u80fd\u7684\u5e7f\uff0c\u663e\u793a\u51fa\u66f4\u591a\u6570\u636e\u7279\u5f81\uff09<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong>\u5728RNA-seq\u4e2d\uff0cPCA\u53ef\u4ee5\u7528\u4e8e\u4ee5\u4e0b\u51e0\u4e2a\u65b9\u9762\uff1a<\/strong><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong><span style=\"color: rgb(249, 110, 87);\">\u6570\u636e\u964d\u7ef4<\/span><\/strong>\uff1a\u901a\u8fc7PCA\u53ef\u4ee5\u5c06\u9ad8\u7ef4\u7684RNA-seq\u6570\u636e\u964d\u7ef4\u5230\u8f83\u4f4e\u7684\u7ef4\u5ea6\uff0c\u540c\u65f6\u4fdd\u7559\u6570\u636e\u7684\u5927\u90e8\u5206\u53d8\u5f02\u6027\u3002\u8fd9\u6837\u53ef\u4ee5\u51cf\u5c11\u6570\u636e\u7684\u566a\u58f0\u548c\u5197\u4f59\u4fe1\u606f\uff0c\u4ece\u800c\u66f4\u597d\u5730\u5c55\u73b0\u6570\u636e\u7684\u4e3b\u8981\u7279\u5f81\u3002<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong><span style=\"color: rgb(249, 110, 87);\">\u53ef\u89c6\u5316<\/span><\/strong>\uff1a\u901a\u8fc7PCA\u53ef\u4ee5\u5c06RNA-seq\u6570\u636e\u53ef\u89c6\u5316\u4e3a\u4e8c\u7ef4\u6216\u4e09\u7ef4\u56fe\u5f62\uff0c\u4ece\u800c\u66f4\u597d\u5730\u7406\u89e3\u6570\u636e\u7684\u7ed3\u6784\u548c\u6a21\u5f0f\u3002\u4f8b\u5982\uff0c\u6211\u4eec\u53ef\u4ee5\u5c06\u4e0d\u540c\u6837\u672c\u4e4b\u95f4\u7684\u57fa\u56e0\u8868\u8fbe\u91cf\u7ed8\u5236\u6210\u4e00\u4e2a\u6563\u70b9\u56fe\uff0c\u4e0d\u540c\u6837\u672c\u7684\u8868\u8fbe\u6a21\u5f0f\u5c31\u53ef\u4ee5\u5728\u56fe\u5f62\u4e2d\u5448\u73b0\u51fa\u6765\u3002<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong><span style=\"color: rgb(249, 110, 87);\">\u6570\u636e\u63a2\u7d22<\/span><\/strong>\uff1a\u901a\u8fc7PCA\u53ef\u4ee5\u53d1\u73b0RNA-seq\u6570\u636e\u7684\u76f8\u5173\u6027\u548c\u5dee\u5f02\u6027\uff0c\u5e2e\u52a9\u6211\u4eec\u53d1\u73b0\u4e0d\u540c\u6837\u672c\u4e4b\u95f4\u7684\u5dee\u5f02\u548c\u5171\u540c\u70b9\u3002\u4f8b\u5982\uff0c\u6211\u4eec\u53ef\u4ee5\u5206\u6790PCA\u56fe\u4e2d\u7684\u6bcf\u4e2a\u4e3b\u6210\u5206\uff0c\u627e\u51fa\u5bf9\u5dee\u5f02\u6027\u6700\u5927\u7684\u57fa\u56e0\uff0c\u8fdb\u4e00\u6b65\u7814\u7a76\u8fd9\u4e9b\u57fa\u56e0\u7684\u751f\u7269\u5b66\u529f\u80fd\u548c\u76f8\u5173\u75be\u75c5\u3002<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\">\u6211\u4eec\u968f\u673a\u751f\u6210\u4e24\u7ec4\u6a21\u62df\u57fa\u56e0\u6570\u636e\uff0c\u5e76\u8fdb\u884cPCA\u964d\u7ef4\u3002<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">set.seed(<span class=\"code-snippet__number\">123<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">n <- 100<\/span><\/code><code><span class=\"code-snippet_outer\">mean_A <- 5<\/span><\/code><code><span class=\"code-snippet_outer\">sd_A <- 2<\/span><\/code><code><span class=\"code-snippet_outer\">mean_B <- 8<\/span><\/code><code><span class=\"code-snippet_outer\">sd_B <- 2<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">gene_data_A <- matrix(rnorm(n * <span class=\"code-snippet__number\">5<\/span>, mean = mean_A, sd = sd_A), nrow = <span class=\"code-snippet__number\">50<\/span>, ncol = <span class=\"code-snippet__number\">10<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">gene_data_B <- matrix(rnorm(n * <span class=\"code-snippet__number\">5<\/span>, mean = mean_B, sd = sd_B), nrow = <span class=\"code-snippet__number\">50<\/span>, ncol = <span class=\"code-snippet__number\">10<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">gene_data <- rbind(gene_data_A, gene_data_B)<\/span><\/code><code><span class=\"code-snippet_outer\">colnames(gene_data) <- paste0(<span class=\"code-snippet__string\">\"Gene\"<\/span>, <span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">10<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">rownames(gene_data) <- <span class=\"code-snippet__number\">1<\/span>:nrow(gene_data)<\/span><\/code><code><span class=\"code-snippet_outer\">group <- data.frame(Sample = <span class=\"code-snippet__number\">1<\/span>:nrow(gene_data), Group = <span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"GroupA\"<\/span>, <span class=\"code-snippet__string\">\"GroupB\"<\/span>), each = <span class=\"code-snippet__number\">50<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u53ef\u4ee5\u76f4\u63a5\u4f7f\u7528FactoMineR \u5305\u8c03\u7528PCA\u3002FactoMineR\u662f\u4e00\u4e2a\u7528\u4e8e\u591a\u5143\u63a2\u7d22\u6027\u6570\u636e\u5206\u6790\u548c\u6570\u636e\u6316\u6398\u7684R\u5305\u3002\u5b83\u4e3a\u591a\u7ef4\u6570\u636e\u7684\u964d\u7ef4\u3001\u805a\u7c7b\u548c\u53ef\u89c6\u5316\u63d0\u4f9b\u4e86\u5e7f\u6cdb\u7684\u65b9\u6cd5\u3002<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">gene.pca <- PCA(gene_data, ncp = <span class=\"code-snippet__number\">2<\/span>, scale.unit = <span class=\"code-snippet__literal\">TRUE<\/span>, graph = <span class=\"code-snippet__literal\">FALSE<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u4f7f\u7528PCA\u964d\u7ef4\uff0c\u63d0\u53d6\u8d21\u732e\u5ea6\u6700\u9ad8\u7684\u4e24\u4e2a\u7279\u5f81<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u63d0\u53d6\u6837\u672c\u5728 PCA \u524d\u4e24\u8f74\u4e2d\u7684\u5750\u6807<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pca_sample <- data.frame(gene.pca$ind$coord[ ,<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">2<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\">pca_sample$Sample=row.names(pca_sample)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u63d0\u53d6 PCA \u524d\u4e24\u8f74\u7684\u8d21\u732e\u5ea6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pca_eig1 <- <span class=\"code-snippet__built_in\">round<\/span>(gene.pca$eig[<span class=\"code-snippet__number\">1<\/span>,<span class=\"code-snippet__number\">2<\/span>], <span class=\"code-snippet__number\">2<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">pca_eig2 <- <span class=\"code-snippet__built_in\">round<\/span>(gene.pca$eig[<span class=\"code-snippet__number\">2<\/span>,<span class=\"code-snippet__number\">2<\/span>],<span class=\"code-snippet__number\">2<\/span> )<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u5bf9\u964d\u7ef4\u7ed3\u679c\u8fdb\u884c\u53ef\u89c6\u5316<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">pca_sample <- merge(pca_sample, group,by=<span class=\"code-snippet__string\">\"Sample\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">head(pca_sample)<\/span><\/code><code><span class=\"code-snippet_outer\">p <- ggplot(data = pca_sample, aes(x = Dim.1, y = Dim.2)) +<\/span><\/code><code><span class=\"code-snippet_outer\">geom_point(aes(color = Group), size = <span class=\"code-snippet__number\">2<\/span>) + <span class=\"code-snippet__comment\">#\u6839\u636e\u6837\u672c\u5750\u6807\u7ed8\u5236\u4e8c\u7ef4\u6563\u70b9\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">scale_color_manual(values = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">'orange'<\/span>, <span class=\"code-snippet__string\">'purple'<\/span>)) + <span class=\"code-snippet__comment\">#\u81ea\u5b9a\u4e49\u989c\u8272<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">theme(panel.grid = element_blank(), panel.background = element_rect(color = <span class=\"code-snippet__string\">'black'<\/span>, fill = <span class=\"code-snippet__string\">'transparent'<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">legend.key = element_rect(fill = <span class=\"code-snippet__string\">'transparent'<\/span>)) + <span class=\"code-snippet__comment\">#\u53bb\u9664\u80cc\u666f\u548c\u7f51\u683c\u7ebf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">labs(x = paste(<span class=\"code-snippet__string\">'PCA1:'<\/span>, pca_eig1, <span class=\"code-snippet__string\">'%'<\/span>), y = paste(<span class=\"code-snippet__string\">'PCA2:'<\/span>, pca_eig2, <span class=\"code-snippet__string\">'%'<\/span>), color = <span class=\"code-snippet__string\">''<\/span>) <span class=\"code-snippet__comment\">#\u5c06 PCA \u8f74\u8d21\u732e\u5ea6\u6dfb\u52a0\u5230\u5750\u6807\u8f74\u6807\u9898\u4e2d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">p<\/span><\/code><code><span class=\"code-snippet_outer\">p + stat_ellipse(aes(color = Group), level = <span class=\"code-snippet__number\">0.95<\/span>, show.legend = <span class=\"code-snippet__literal\">FALSE<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">p + stat_ellipse(aes(fill = Group), geom = <span class=\"code-snippet__string\">'polygon'<\/span>, level = <span class=\"code-snippet__number\">0.95<\/span>, alpha = <span class=\"code-snippet__number\">0.3<\/span>, show.legend = <span class=\"code-snippet__literal\">FALSE<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">scale_fill_manual(values = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">'orange'<\/span>, <span class=\"code-snippet__string\">'purple'<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7045929018789144\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_z8eAFtm0qH9oj5FKnqicVOKdXCibSg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEWaOVp4oK5ibDkBelm3NzicicQLtFC0YNLByA2c2WqnOcUveZiaNicz8eAFtm0qH9oj5FKnqicVOKdXCibSg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"958\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u8fd9\u5c31\u662f\u4f7f\u7528R\u5bf9\u591a\u7ef4\u7684\u57fa\u56e0\u6570\u636e\u8fdb\u884cPCA\u7684\u8fc7\u7a0b\u5566\uff0c\u662f\u4e0d\u662f\u975e\u5e38\u7b80\u5355\uff01\u5feb\u548c\u5c0f\u5e08\u59b9\u4e00\u8d77\u8bd5\u8bd5\u5427\uff01<\/p>\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;height: auto;align-self: center;margin-right: 10px;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(249, 172, 172);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;border-style: solid;border-width: 1px;border-color: rgb(249, 172, 172);padding-right: 8px;padding-left: 8px;margin-right: 10px;\">\n<section style=\"text-align: justify;color: rgb(249, 172, 172);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">E<\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;border-style: solid;border-width: 1px;border-color: rgb(249, 172, 172);padding-right: 8px;padding-left: 8px;margin-right: 10px;\">\n<section style=\"text-align: justify;color: rgb(249, 172, 172);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">N<\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;border-style: solid;border-width: 1px;border-color: rgb(249, 172, 172);padding-right: 8px;padding-left: 8px;margin-right: 10px;\">\n<section style=\"text-align: justify;color: rgb(249, 172, 172);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">D<\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 100 100 0%;height: auto;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(249, 172, 172);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-align: center;margin-bottom: 24px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5555555555555556\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_icWAmgGMVEzREAJmM59ov44ziaMCGg-5.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/oCSOtP61aEWjRWAeR9wicY9JyrK13cNBaR2PPYHOsBTrS4rJOUOnJCEIxt1icWAmgGMVEzREAJmM59ov44ziaMCGg\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"900\" style=\"\" \/><\/p>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u57fa\u56e0\u7ef4\u5ea6\u592a\u9ad8\u600e\u4e48\u529e\uff1f\u65e0\u6240\u8c13\uff01PCA\u964d\u7ef4\u4f1a\u51fa\u624b\uff01 \u5c0f\u5e08\u59b9 \u751f\u4fe1\u679c 2 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55181"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=55181"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55181\/revisions"}],"predecessor-version":[{"id":55184,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55181\/revisions\/55184"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=55181"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=55181"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=55181"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}