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data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"pySCENIC\u5bf9\u5355\u7ec6\u80de\u8f6c\u5f55\u56e0\u5b50\u5206\u6790\u53ef\u89c6\u5316\uff08\u4e0b\uff09\",\"time\":\"2023-08-13 19:02:06\",\"timeStamp\":\"1691924526\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491422&idx=4&sn=cbf386d9fcc16596567738edd0314327&chksm=cf7c8f9bf80b068de6c9e2a7cd1f2a825e173ae0abd4ceb931d417a16e7d8593582e64994817#rd\" target=\"_blank\" rel=\"noopener\">pySCENIC\u5bf9\u5355\u7ec6\u80de\u8f6c\u5f55\u56e0\u5b50\u5206\u6790\u53ef\u89c6\u5316\uff08\u4e0b\uff09<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span class=\"rich_media_meta rich_media_meta_text\">\u5927\u6d77\u54e5 <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" 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row;margin: -9px 0% 5px;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;align-self: flex-start;border-width: 0px;margin-top: 5px;margin-left: 7px;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 2px;height: 10px;vertical-align: top;overflow: hidden;background-color: rgb(59, 149, 115);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"text-align: center;margin: -6px 0% 4px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 10px;height: 2px;vertical-align: top;overflow: hidden;background-color: rgb(59, 149, 115);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;border-width: 0px;margin-left: 4px;\">\n<section style=\"text-align: center;margin-top: -4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 9px;height: 9px;vertical-align: top;overflow: hidden;border-width: 2px;border-radius: 47px;border-style: solid;border-color: rgb(59, 149, 115);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;\">\n<section style=\"margin-right: 0%;margin-left: 0%;text-align: center;transform: translate3d(-1px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 19px;padding-right: 11px;padding-left: 11px;line-height: 1;text-align: justify;\">\n<p style=\"text-align: left;text-wrap: wrap;\"><strong>\u70b9\u51fb\u84dd\u5b57 \u5173\u6ce8\u6211\u4eec<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;line-height: 0;flex: 0 0 0%;height: auto;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(59, 149, 115);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: center;transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(59, 149, 115);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p>SCENIC(Single-Cell rEgulatory Network Inference and Clustering)\u662f\u4e00\u4e2a\u57fa\u4e8e\u5171\u8868\u8fbe\u548cmotif\u5206\u6790\uff0c\u7528\u4e8e\u8ba1\u7b97\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6570\u636e\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u91cd\u5efa\u53ca\u7ec6\u80de\u72b6\u6001\u9274\u5b9a\u7684\u65b9\u6cd5\uff0c\u53ef\u4ee5\u7b80\u5355\u7406\u89e3\u4e3a\u5355\u7ec6\u80de\u8f6c\u5f55\u56e0\u5b50\u5206\u6790\u3002<\/p>\n<p><br \/><\/p>\n<p>\u5b98\u65b9\u6559\u7a0bhttps:\/\/github.com\/aertslab\/SCENIC<\/p>\n<p>\u8fd9\u91cc\u4f7f\u7528python\u7248\u672c\u7684pyscenic\u548cR\u53ef\u89c6\u5316\u76f8\u7ed3\u5408\u7684\u65b9\u6cd5\uff0c\u5229\u7528\u5355\u7ec6\u80de\u6ce8\u91ca\u5b8c\u6574\u7684Seurat\u5bf9\u8c61\uff0c\u5f97\u5230\u4e0d\u540c\u7ec4\u522b\u548c\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u4e2d\u9ad8\u8868\u8fbe\u7684\u8f6c\u5f55\u56e0\u5b50\u70ed\u56fe\u3002<\/p>\n<p><br \/><\/p>\n<p>\u6211\u4eec\u5728\u4e0a\u7bc7\u63a8\u9001\u4e2d\u5f97\u5230\u4e86\u6240\u9700\u8981\u7684sample_SCENIC.loom\u6587\u4ef6\uff0c\u8fd9\u91cc\u6211\u4eec\u5c06\u5176\u5bfc\u5165R\uff0c\u5e76\u7ed3\u5408Seurat\u5bf9\u8c61\u7684\u4fe1\u606f\u8fdb\u884c\u53ef\u89c6\u5316\u3002<\/p>\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p><strong>\u9996\u5148\u52a0\u8f7d\u9700\u8981\u7684R\u5305<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">library(tidyverse)<\/span><\/code><code><span class=\"code-snippet_outer\">library(Seurat)<\/span><\/code><code><span class=\"code-snippet_outer\">library(GENIE3) <span class=\"code-snippet__comment\">#\u57fa\u4e8e\u5171\u8868\u8fbe\u60c5\u51b5\u9274\u5b9a\u6bcf\u4e2aTF\u7684\u6f5c\u5728\u9776\u70b9\uff0c\u63a8\u65ad\u5171\u8868\u8fbe\u7f51\u7edc<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(RcisTarget) <span class=\"code-snippet__comment\">#\u57fa\u4e8eDNA-motif \u5206\u6790\u9009\u62e9\u6f5c\u5728\u7684\u76f4\u63a5\u7ed3\u5408\u9776\u70b9\uff0c\u8fdb\u884c\u8f6c\u5f55\u56e0\u5b50\u7ed3\u5408\u7684motif\u5206\u6790<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(AUCell) <span class=\"code-snippet__comment\">#\u9274\u5b9a\u5177\u6709\u6fc0\u6d3b\u57fa\u56e0\u96c6\u6216\u57fa\u56e0\u7f51\u7edc\u7684\u7ec6\u80de\uff0c\u5206\u6790\u6bcf\u4e2a\u7ec6\u80de\u7684\u7f51\u7edc\u6d3b\u52a8\u5ea6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(SCENIC)<\/span><\/code><code><span class=\"code-snippet_outer\">library(SCopeLoomR) <span class=\"code-snippet__comment\">#\u8bfb\u53d6loom\u6587\u4ef6\u9700\u8981\u7528\u5230<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4ee5\u4e0a\u5305\u5b89\u88c5\u7684\u65f6\u5019\u53ef\u80fd\u4f1a\u9047\u5230\u7f16\u8bd1\u62a5\u9519\uff0c\u5927\u5bb6\u540e\u7eed\u5982\u679c\u6709\u95ee\u9898\u7684\u8bdd\u8054\u7cfb\u5c0f\u679c\u51fa\u6559\u7a0b\u54e6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(RColorBrewer) <span class=\"code-snippet__comment\">#\u7ed8\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(patchwork) <span class=\"code-snippet__comment\">#\u7ed8\u56fe<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p><strong>\u5bfc\u5165\u6570\u636e<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">loom <- open_loom(<span class=\"code-snippet__string\">\"sample_SCENIC.loom\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;\">\n<p><strong>\u63a5\u4e0b\u6765\u65e0\u8111\u8fd0\u884c\u5c31\u597d\uff0c<\/strong><\/p>\n<p><strong>\u6709\u5174\u8da3\u4e86\u89e3\u7684\u53ef\u4ee5\u53bb\u5b98\u7f51\u7814\u7a76<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">regulons_incidMat <- get_regulons(loom, column.attr.name=<span class=\"code-snippet__string\">\"Regulons\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">regulons <- regulonsToGeneLists(regulons_incidMat)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">class<\/span>(regulons)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">regulonAUC <- get_regulons_AUC(loom, column.attr.name=<span class=\"code-snippet__string\">'RegulonsAUC'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">regulonAucThresholds <- get_regulon_thresholds(loom)<\/span><\/code><code><span class=\"code-snippet_outer\">tail(regulonAucThresholds [order(<span class=\"code-snippet__built_in\">as.numeric<\/span>(<span class=\"code-snippet__built_in\">names<\/span>(regulonAucThresholds)))])<\/span><\/code><code><span class=\"code-snippet_outer\">embeddings <- get_embeddings(loom)<\/span><\/code><code><span class=\"code-snippet_outer\">close_loom(loom)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p><strong>\u5bfc\u5165Seurat\u5bf9\u8c61<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">\u4ee5\u4e0a\u76ae\u7ec6\u80de\u4e3a\u4f8b\uff0c\u4eceSeurat\u5bf9\u8c61\uff08sce\uff09\u4e2d\u63d0\u53d6\u51fa\u6765\u3002\u8981\u6ce8\u610f\u7684\u662f\u7ec6\u80de\u6570\u6700\u597d\u5728\u4e00\u4e07\u4ee5\u4e0b\uff0c\u5426\u5219\u8fd0\u884c\u65f6\u957f\u4e0d\u53ef\u60f3\u8c61\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">epithe <- subset(sce, idents = <span class=\"code-snippet__string\">\"epithelial\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">sub_regulonAUC <- regulonAUC [,match(colnames(epithe),colnames(regulonAUC))]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">dim<\/span>(sub_regulonAUC)<\/span><\/code><code><span class=\"code-snippet_outer\">identical(colnames(sub_regulonAUC), colnames(epithe))<\/span><\/code><code><span class=\"code-snippet_outer\">capture.output(regulons,file=<span class=\"code-snippet__string\">\"TF2gene.txt\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">TFhigh <- <span class=\"code-snippet__built_in\">names<\/span>(regulons)<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(TFhigh,file=<span class=\"code-snippet__string\">\"TFhigh.txt\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">save(sub_regulonAUC,file=<span class=\"code-snippet__string\">\"AUCellresult.RData\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">regulonActivity_byCellType <- sapply(split(colnames(epithe), epithe$celltype),<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__keyword\">function<\/span>(cells), rowMeans(AUC[,cells]))<\/span><\/code><code><span class=\"code-snippet_outer\">regulonActivity_byCellType_Scaled <- t(scale(t(regulonActivity_byCellType), center = <span class=\"code-snippet__built_in\">T<\/span>, scale=<span class=\"code-snippet__built_in\">T<\/span>))))<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p style=\"text-align: left;text-wrap: wrap;\"><strong>\u4fdd\u5b58\u4e00\u4e0b<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">AUC <- getAUC(regulonAUC)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">colnames(AUC) <- epithe$group<\/span><\/code><code><span class=\"code-snippet_outer\">AUC _sub <- AUC [rownames(AUC) %in% <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"BATF(+)\"<\/span>\u2026\u2026,] <span class=\"code-snippet__comment\"># TFhigh.txt\u91cc\u9009\u51fa\u7684TF<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">dim<\/span>(AUC_sub)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p style=\"text-align: left;text-wrap: wrap;\"><strong>\u5f00\u59cb\u7ed8\u56fe<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">annotation_col = data.frame(CellType = epithe$celltype, <\/span><\/code><code><span class=\"code-snippet_outer\"> Group = epithe$group)<\/span><\/code><code><span class=\"code-snippet_outer\">ann_colors=<span class=\"code-snippet__built_in\">list<\/span>(Group=<span class=\"code-snippet__built_in\">c<\/span>(N=<span class=\"code-snippet__string\">'blue'<\/span>,<span class=\"code-snippet__built_in\">T<\/span>=<span class=\"code-snippet__string\">'red'<\/span>)) <span class=\"code-snippet__comment\">#\u4e3a\u4e0d\u540c\u7ec4\u522b\u6807\u6ce8\u4e0d\u540c\u7684\u989c\u8272<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pdf(<span class=\"code-snippet__string\">'SCENIC_heatmap.pdf'<\/span>, width =<span class=\"code-snippet__number\">10<\/span>, height = <span class=\"code-snippet__number\">3<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">pheatmap(AUC_sub, <\/span><\/code><code><span class=\"code-snippet_outer\"> col= rev(brewer.pal(<span class=\"code-snippet__number\">5<\/span>, <span class=\"code-snippet__string\">'RdBu'<\/span>)),<\/span><\/code><code><span class=\"code-snippet_outer\"> show_rownames=<span class=\"code-snippet__built_in\">T<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> annotation_colors=ann_colors,<\/span><\/code><code><span class=\"code-snippet_outer\"> annotation_col = annotation_col,<\/span><\/code><code><span class=\"code-snippet_outer\"> cutree_col=<span class=\"code-snippet__number\">3<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">dev.off()<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p>\u8fd9\u6837\u5c31\u5b8c\u6210\u9ad8\u8868\u8fbe\u7684\u8f6c\u5f55\u56e0\u5b50\u70ed\u56fe\u7684\u53ef\u89c6\u5316\u5566~\u8ba9\u6211\u4eec\u770b\u770b<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.2808219178082192\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_qCfNoaEcjVZSZOXib54TnQVHCnkatg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSibtO4W6uSCfpGmYia6GTh9S1mdDX7FF6vaYBmYF8YX74zuPxqCfNoaEcjVZSZOXib54TnQVHCnkatg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1022\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>#\u56fe\u7247\u6765\u6e90\uff1aYao J, et al. Single-Cell RNA-Seq Reveals the Promoting Role of Ferroptosis Tendency During Lung Adenocarcinoma EMT Progression. Front Cell Dev Biol. 2021<\/p>\n<p><br \/><\/p>\n<p>\u524d\u9762\u6211\u4eec\u63d0\u5230\uff0cSCENIC\u5360\u7528\u5927\u3001\u8017\u65f6\u957f\uff0c\u800c\u5c0f\u679c\u8fd9\u6b21\u4ecb\u7ecd\u7684pySCENIC\u901f\u5ea6\u6bd4R\u7248\u672c\u7684\u6709\u5341\u5206\u663e\u8457\u7684\u4f18\u52bf\uff01\u5b66\u4f1apySCENIC\u8ba9\u4f60\u5feb\u4eba\u4e00\u6b65\uff01<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3824074074074074\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-13_\u300c\u8f6c\u300dpySCENIC\u5bf9\u5355\u7ec6\u80de\u8f6c\u5f55\u56e0\u5b50\u5206\u6790\u53ef\u89c6\u5316\uff08\u4e0b\uff09\/2_h5YsicpkVJhg8kTg4kufoaoicoUpEQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSibtO4W6uSCfpGmYia6GTh9SRlLydwHtfN0ojwI8prqaoTJzyDh5YsicpkVJhg8kTg4kufoaoicoUpEQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"display: flex;flex-flow: row;margin: 10px 0% 20px;text-align: left;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;align-self: flex-start;line-height: 0;\">\n<section style=\"text-align: right;margin-right: 0%;margin-bottom: -20px;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 8px;height: 20px;vertical-align: top;overflow: hidden;border-width: 0px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 241, 224);\"><svg viewbox=\"0 0 1 1\" 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14px;line-height: 1.8;letter-spacing: 2px;\">\n<p>\u600e\u4e48\u6837\uff0c\u4f60\u5b66\u4f1a\u4e86\u5417\uff1f\u8fd8\u6709\u5176\u5b83\u4e00\u4e9b\u5b9e\u7528\u65b9\u4fbf\u7684\u5c0f\u5de5\u5177\u5728\u4e91\u751f\u4fe1\u5e73\u53f0\u7b49\u7740\u4f60\u54e6<\/p>\n<p><span style=\"color: rgb(59, 149, 115);\"><strong>http:\/\/www.biocloudservice.com\/home.html<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"border-bottom: 2px solid rgb(0, 150, 136);text-align: left;font-size: 14px;\" powered-by=\"xiumi.us\">\n<p>\u70b9\u51fb\u201c\u9605\u8bfb\u539f\u6587\u201d\u8fdb\u5165\u7f51\u5740<\/p>\n<\/section>\n<section style=\"margin-left: 8%;margin-right: 8%;line-height: 1.1em;\" powered-by=\"xiumi.us\">\n<section style=\"width: 0px;display: inline-block;vertical-align: top;border-top: 1em solid rgb(0, 150, 136);border-left: 0.8em solid transparent !important;border-right: 0.8em solid transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<p style=\"margin-bottom: 24px;\"><br \/><\/p>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>pySCENIC\u5bf9\u5355\u7ec6\u80de\u8f6c\u5f55\u56e0\u5b50\u5206\u6790\u53ef\u89c6\u5316\uff08\u4e0b\uff09 \u5927\u6d77\u54e5 \u751f\u4fe1\u679c 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{"id":55360,"date":"2024-06-16T16:27:02","date_gmt":"2024-06-16T08:27:02","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55360"},"modified":"2024-06-16T16:27:02","modified_gmt":"2024-06-16T08:27:02","slug":"pyscenic%e5%af%b9%e5%8d%95%e7%bb%86%e8%83%9e%e8%bd%ac%e5%bd%95%e5%9b%a0%e5%ad%90%e5%88%86%e6%9e%90%e5%8f%af%e8%a7%86%e5%8c%96%ef%bc%88%e4%b8%8b%ef%bc%89-2","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55360","title":{"rendered":"pySCENIC\u5bf9\u5355\u7ec6\u80de\u8f6c\u5f55\u56e0\u5b50\u5206\u6790\u53ef\u89c6\u5316\uff08\u4e0b\uff09"},"content":{"rendered":"

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#33ab01;margin-right:5px;}.ad{text-decoration:none;color:#d6d4d4;font-size:12px;}.msgBodyReply{padding-top:5px;}.userName span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation 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.code-snippet__title{color:#0e9ce5}.code-snippet__tag,.code-snippet__name,.code-snippet__attribute{color:#0e9ce5;font-weight:normal}.code-snippet__regexp,.code-snippet__link{color:#ca7d37}.code-snippet__symbol,.code-snippet__bullet{color:#d14}.code-snippet__built_in,.code-snippet__builtin-name{color:#ca7d37}.code-snippet__meta{color:#afafaf}.code-snippet__deletion{background:#fdd}.code-snippet__addition{background:#dfd}.code-snippet__emphasis{font-style:italic}.code-snippet__strong{font-weight:bold}.account_avatar{width:40px;height:40px;padding:0;}.account_info{display:-webkit-box;display:-webkit-flex;display:flex;-webkit-box-align:center;-webkit-align-items:center;padding:20px 0;align-items:center}.flex_bd{padding-left:14px;}.account_nickname{display:inline-block;vertical-align:middle;line-height:1.2;color:#576b95;font-size:14px}.account_desc{overflow:hidden;text-overflow:ellipsis;display:-webkit-box;-webkit-box-orient:vertical;-webkit-line-clamp:1;color:rgba(0,0,0,0.3);font-size:14px;line-height:1.2;padding-top:.4em}.msg_source_url{text-align:left;word-break:break-all;margin-top:20px;}.msg_source_url a{padding-right:10px;}.msg_source_url .url_text{color:#a8a8a8;}.video-desc{font-size:14px;margin-top:15px;color:#6c6c6c;}.msg_source_url{text-align:left;}.original_primary_card_tips{color:rgba(0,0,0,0.3);line-height:1.4;font-size:15px;}.weui-flex__item{margin-bottom:20px;padding:20px 16px;margin-top:16px;line-height:1.4;align-items:center;background-color:#f7f7f7;border-radius:8px;position:relative;}.original_primary_desc{color:rgba(0,0,0,0.5);font-size:14px;padding-top:4px;width:auto;overflow:hidden;text-overflow:ellipsis;}.msgBodyReplyList{border-top:1px solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"pySCENIC\u5bf9\u5355\u7ec6\u80de\u8f6c\u5f55\u56e0\u5b50\u5206\u6790\u53ef\u89c6\u5316\uff08\u4e0b\uff09\",\"time\":\"2023-08-13 19:02:06\",\"timeStamp\":\"1691924526\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491422&idx=4&sn=cbf386d9fcc16596567738edd0314327&chksm=cf7c8f9bf80b068de6c9e2a7cd1f2a825e173ae0abd4ceb931d417a16e7d8593582e64994817#rd\" target=\"_blank\" rel=\"noopener\">pySCENIC\u5bf9\u5355\u7ec6\u80de\u8f6c\u5f55\u56e0\u5b50\u5206\u6790\u53ef\u89c6\u5316\uff08\u4e0b\uff09<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span class=\"rich_media_meta rich_media_meta_text\">\u5927\u6d77\u54e5 <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-08-13 19:02:06<\/em><\/div>\n<p><content><\/p>\n<div class=\"reprint\">\n<p>\u8f6c\u81ea\u516c\u4f17\u53f7\uff1a\u751f\u4fe1\u6ee9<br \/><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzkzMzUwODcwMg==&mid=2247484206&idx=1&sn=b2b2c697136362d55cd162f1ce7a40ed\" target=\"_blank\" title=\"\u9605\u8bfb\u539f\u6587\" rel=\"noopener\">http:\/\/mp.weixin.qq.com\/s?__biz=MzkzMzUwODcwMg==&mid=2247484206&idx=1&sn=b2b2c697136362d55cd162f1ce7a40ed<\/a><\/p>\n<\/div>\n<section style=\"font-size: 16px;margin-bottom: 24px;\">\n<section style=\"text-align: left;justify-content: flex-start;margin: 25px 0% 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(245, 241, 224);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"display: flex;flex-flow: row;margin: -9px 0% 5px;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;align-self: flex-start;border-width: 0px;margin-top: 5px;margin-left: 7px;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 2px;height: 10px;vertical-align: top;overflow: hidden;background-color: rgb(59, 149, 115);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"text-align: center;margin: -6px 0% 4px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 10px;height: 2px;vertical-align: top;overflow: hidden;background-color: rgb(59, 149, 115);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;border-width: 0px;margin-left: 4px;\">\n<section style=\"text-align: center;margin-top: -4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 9px;height: 9px;vertical-align: top;overflow: hidden;border-width: 2px;border-radius: 47px;border-style: solid;border-color: rgb(59, 149, 115);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;\">\n<section style=\"margin-right: 0%;margin-left: 0%;text-align: center;transform: translate3d(-1px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 19px;padding-right: 11px;padding-left: 11px;line-height: 1;text-align: justify;\">\n<p style=\"text-align: left;text-wrap: wrap;\"><strong>\u70b9\u51fb\u84dd\u5b57 \u5173\u6ce8\u6211\u4eec<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;line-height: 0;flex: 0 0 0%;height: auto;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(59, 149, 115);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: center;transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(59, 149, 115);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p>SCENIC(Single-Cell rEgulatory Network Inference and Clustering)\u662f\u4e00\u4e2a\u57fa\u4e8e\u5171\u8868\u8fbe\u548cmotif\u5206\u6790\uff0c\u7528\u4e8e\u8ba1\u7b97\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6570\u636e\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u91cd\u5efa\u53ca\u7ec6\u80de\u72b6\u6001\u9274\u5b9a\u7684\u65b9\u6cd5\uff0c\u53ef\u4ee5\u7b80\u5355\u7406\u89e3\u4e3a\u5355\u7ec6\u80de\u8f6c\u5f55\u56e0\u5b50\u5206\u6790\u3002<\/p>\n<p><br \/><\/p>\n<p>\u5b98\u65b9\u6559\u7a0bhttps:\/\/github.com\/aertslab\/SCENIC<\/p>\n<p>\u8fd9\u91cc\u4f7f\u7528python\u7248\u672c\u7684pyscenic\u548cR\u53ef\u89c6\u5316\u76f8\u7ed3\u5408\u7684\u65b9\u6cd5\uff0c\u5229\u7528\u5355\u7ec6\u80de\u6ce8\u91ca\u5b8c\u6574\u7684Seurat\u5bf9\u8c61\uff0c\u5f97\u5230\u4e0d\u540c\u7ec4\u522b\u548c\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u4e2d\u9ad8\u8868\u8fbe\u7684\u8f6c\u5f55\u56e0\u5b50\u70ed\u56fe\u3002<\/p>\n<p><br \/><\/p>\n<p>\u6211\u4eec\u5728\u4e0a\u7bc7\u63a8\u9001\u4e2d\u5f97\u5230\u4e86\u6240\u9700\u8981\u7684sample_SCENIC.loom\u6587\u4ef6\uff0c\u8fd9\u91cc\u6211\u4eec\u5c06\u5176\u5bfc\u5165R\uff0c\u5e76\u7ed3\u5408Seurat\u5bf9\u8c61\u7684\u4fe1\u606f\u8fdb\u884c\u53ef\u89c6\u5316\u3002<\/p>\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p><strong>\u9996\u5148\u52a0\u8f7d\u9700\u8981\u7684R\u5305<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">library(tidyverse)<\/span><\/code><code><span class=\"code-snippet_outer\">library(Seurat)<\/span><\/code><code><span class=\"code-snippet_outer\">library(GENIE3) <span class=\"code-snippet__comment\">#\u57fa\u4e8e\u5171\u8868\u8fbe\u60c5\u51b5\u9274\u5b9a\u6bcf\u4e2aTF\u7684\u6f5c\u5728\u9776\u70b9\uff0c\u63a8\u65ad\u5171\u8868\u8fbe\u7f51\u7edc<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(RcisTarget) <span class=\"code-snippet__comment\">#\u57fa\u4e8eDNA-motif \u5206\u6790\u9009\u62e9\u6f5c\u5728\u7684\u76f4\u63a5\u7ed3\u5408\u9776\u70b9\uff0c\u8fdb\u884c\u8f6c\u5f55\u56e0\u5b50\u7ed3\u5408\u7684motif\u5206\u6790<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(AUCell) <span class=\"code-snippet__comment\">#\u9274\u5b9a\u5177\u6709\u6fc0\u6d3b\u57fa\u56e0\u96c6\u6216\u57fa\u56e0\u7f51\u7edc\u7684\u7ec6\u80de\uff0c\u5206\u6790\u6bcf\u4e2a\u7ec6\u80de\u7684\u7f51\u7edc\u6d3b\u52a8\u5ea6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(SCENIC)<\/span><\/code><code><span class=\"code-snippet_outer\">library(SCopeLoomR) <span class=\"code-snippet__comment\">#\u8bfb\u53d6loom\u6587\u4ef6\u9700\u8981\u7528\u5230<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4ee5\u4e0a\u5305\u5b89\u88c5\u7684\u65f6\u5019\u53ef\u80fd\u4f1a\u9047\u5230\u7f16\u8bd1\u62a5\u9519\uff0c\u5927\u5bb6\u540e\u7eed\u5982\u679c\u6709\u95ee\u9898\u7684\u8bdd\u8054\u7cfb\u5c0f\u679c\u51fa\u6559\u7a0b\u54e6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(RColorBrewer) <span class=\"code-snippet__comment\">#\u7ed8\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(patchwork) <span class=\"code-snippet__comment\">#\u7ed8\u56fe<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p><strong>\u5bfc\u5165\u6570\u636e<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">loom <- open_loom(<span class=\"code-snippet__string\">\"sample_SCENIC.loom\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;\">\n<p><strong>\u63a5\u4e0b\u6765\u65e0\u8111\u8fd0\u884c\u5c31\u597d\uff0c<\/strong><\/p>\n<p><strong>\u6709\u5174\u8da3\u4e86\u89e3\u7684\u53ef\u4ee5\u53bb\u5b98\u7f51\u7814\u7a76<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">regulons_incidMat <- get_regulons(loom, column.attr.name=<span class=\"code-snippet__string\">\"Regulons\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">regulons <- regulonsToGeneLists(regulons_incidMat)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">class<\/span>(regulons)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">regulonAUC <- get_regulons_AUC(loom, column.attr.name=<span class=\"code-snippet__string\">'RegulonsAUC'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">regulonAucThresholds <- get_regulon_thresholds(loom)<\/span><\/code><code><span class=\"code-snippet_outer\">tail(regulonAucThresholds [order(<span class=\"code-snippet__built_in\">as.numeric<\/span>(<span class=\"code-snippet__built_in\">names<\/span>(regulonAucThresholds)))])<\/span><\/code><code><span class=\"code-snippet_outer\">embeddings <- get_embeddings(loom)<\/span><\/code><code><span class=\"code-snippet_outer\">close_loom(loom)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p><strong>\u5bfc\u5165Seurat\u5bf9\u8c61<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">\u4ee5\u4e0a\u76ae\u7ec6\u80de\u4e3a\u4f8b\uff0c\u4eceSeurat\u5bf9\u8c61\uff08sce\uff09\u4e2d\u63d0\u53d6\u51fa\u6765\u3002\u8981\u6ce8\u610f\u7684\u662f\u7ec6\u80de\u6570\u6700\u597d\u5728\u4e00\u4e07\u4ee5\u4e0b\uff0c\u5426\u5219\u8fd0\u884c\u65f6\u957f\u4e0d\u53ef\u60f3\u8c61\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">epithe <- subset(sce, idents = <span class=\"code-snippet__string\">\"epithelial\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">sub_regulonAUC <- regulonAUC [,match(colnames(epithe),colnames(regulonAUC))]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">dim<\/span>(sub_regulonAUC)<\/span><\/code><code><span class=\"code-snippet_outer\">identical(colnames(sub_regulonAUC), colnames(epithe))<\/span><\/code><code><span class=\"code-snippet_outer\">capture.output(regulons,file=<span class=\"code-snippet__string\">\"TF2gene.txt\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">TFhigh <- <span class=\"code-snippet__built_in\">names<\/span>(regulons)<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(TFhigh,file=<span class=\"code-snippet__string\">\"TFhigh.txt\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">save(sub_regulonAUC,file=<span class=\"code-snippet__string\">\"AUCellresult.RData\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">regulonActivity_byCellType <- sapply(split(colnames(epithe), epithe$celltype),<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__keyword\">function<\/span>(cells), rowMeans(AUC[,cells]))<\/span><\/code><code><span class=\"code-snippet_outer\">regulonActivity_byCellType_Scaled <- t(scale(t(regulonActivity_byCellType), center = <span class=\"code-snippet__built_in\">T<\/span>, scale=<span class=\"code-snippet__built_in\">T<\/span>))))<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p style=\"text-align: left;text-wrap: wrap;\"><strong>\u4fdd\u5b58\u4e00\u4e0b<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">AUC <- getAUC(regulonAUC)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">colnames(AUC) <- epithe$group<\/span><\/code><code><span class=\"code-snippet_outer\">AUC _sub <- AUC [rownames(AUC) %in% <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"BATF(+)\"<\/span>\u2026\u2026,] <span class=\"code-snippet__comment\"># TFhigh.txt\u91cc\u9009\u51fa\u7684TF<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">dim<\/span>(AUC_sub)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p style=\"text-align: left;text-wrap: wrap;\"><strong>\u5f00\u59cb\u7ed8\u56fe<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">annotation_col = data.frame(CellType = epithe$celltype, <\/span><\/code><code><span class=\"code-snippet_outer\"> Group = epithe$group)<\/span><\/code><code><span class=\"code-snippet_outer\">ann_colors=<span class=\"code-snippet__built_in\">list<\/span>(Group=<span class=\"code-snippet__built_in\">c<\/span>(N=<span class=\"code-snippet__string\">'blue'<\/span>,<span class=\"code-snippet__built_in\">T<\/span>=<span class=\"code-snippet__string\">'red'<\/span>)) <span class=\"code-snippet__comment\">#\u4e3a\u4e0d\u540c\u7ec4\u522b\u6807\u6ce8\u4e0d\u540c\u7684\u989c\u8272<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pdf(<span class=\"code-snippet__string\">'SCENIC_heatmap.pdf'<\/span>, width =<span class=\"code-snippet__number\">10<\/span>, height = <span class=\"code-snippet__number\">3<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">pheatmap(AUC_sub, <\/span><\/code><code><span class=\"code-snippet_outer\"> col= rev(brewer.pal(<span class=\"code-snippet__number\">5<\/span>, <span class=\"code-snippet__string\">'RdBu'<\/span>)),<\/span><\/code><code><span class=\"code-snippet_outer\"> show_rownames=<span class=\"code-snippet__built_in\">T<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> annotation_colors=ann_colors,<\/span><\/code><code><span class=\"code-snippet_outer\"> annotation_col = annotation_col,<\/span><\/code><code><span class=\"code-snippet_outer\"> cutree_col=<span class=\"code-snippet__number\">3<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">dev.off()<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p>\u8fd9\u6837\u5c31\u5b8c\u6210\u9ad8\u8868\u8fbe\u7684\u8f6c\u5f55\u56e0\u5b50\u70ed\u56fe\u7684\u53ef\u89c6\u5316\u5566~\u8ba9\u6211\u4eec\u770b\u770b<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.2808219178082192\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_qCfNoaEcjVZSZOXib54TnQVHCnkatg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSibtO4W6uSCfpGmYia6GTh9S1mdDX7FF6vaYBmYF8YX74zuPxqCfNoaEcjVZSZOXib54TnQVHCnkatg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1022\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>#\u56fe\u7247\u6765\u6e90\uff1aYao J, et al. Single-Cell RNA-Seq Reveals the Promoting Role of Ferroptosis Tendency During Lung Adenocarcinoma EMT Progression. Front Cell Dev Biol. 2021<\/p>\n<p><br \/><\/p>\n<p>\u524d\u9762\u6211\u4eec\u63d0\u5230\uff0cSCENIC\u5360\u7528\u5927\u3001\u8017\u65f6\u957f\uff0c\u800c\u5c0f\u679c\u8fd9\u6b21\u4ecb\u7ecd\u7684pySCENIC\u901f\u5ea6\u6bd4R\u7248\u672c\u7684\u6709\u5341\u5206\u663e\u8457\u7684\u4f18\u52bf\uff01\u5b66\u4f1apySCENIC\u8ba9\u4f60\u5feb\u4eba\u4e00\u6b65\uff01<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3824074074074074\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-08-13_\u300c\u8f6c\u300dpySCENIC\u5bf9\u5355\u7ec6\u80de\u8f6c\u5f55\u56e0\u5b50\u5206\u6790\u53ef\u89c6\u5316\uff08\u4e0b\uff09\/2_h5YsicpkVJhg8kTg4kufoaoicoUpEQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSibtO4W6uSCfpGmYia6GTh9SRlLydwHtfN0ojwI8prqaoTJzyDh5YsicpkVJhg8kTg4kufoaoicoUpEQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"display: flex;flex-flow: row;margin: 10px 0% 20px;text-align: left;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;align-self: flex-start;line-height: 0;\">\n<section style=\"text-align: right;margin-right: 0%;margin-bottom: -20px;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 8px;height: 20px;vertical-align: top;overflow: hidden;border-width: 0px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 241, 224);\"><svg viewbox=\"0 0 1 1\" 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