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class=\"rich_pages wxw-img\" data-ratio=\"0.4787037037037037\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_IoNdd2HicoT9zkO7hA93skwY9rxVDg-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYicCDZicoI5eibk0ibO86WM0WsWYwqIoNdd2HicoT9zkO7hA93skwY9rxVDg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>\u901a\u8fc7\u8fdb\u884c\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\uff0c\u5c31\u80fd\u77e5\u9053\u4e00\u7247\u7ec4\u7ec7\u7684\u7ec6\u80de\u7c7b\u578b\uff0c\u53ca\u5176\u4f4d\u7f6e\uff0c\u8fd9\u5bf9\u7814\u7a76\u80bf\u7624\u7ec4\u7ec7\u4f1a\u6709\u5f88\u5927\u7684\u5e2e\u52a9\u3002<\/p>\n<p>10\u00d7genomics Visium \u7a7a\u95f4\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\u5e73\u53f0<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3388888888888889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_3DsibSQ9cXc0a8lzQYWlZ67o4WJjlg-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYAw9fubNp7XpwogkdsObiak4tof3DsibSQ9cXc0a8lzQYWlZ67o4WJjlg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">10x Visium \u4f7f\u7528\u7279\u5236\u7684\u73bb\u7247\uff0c\u73bb\u7247\u4e0a\u6709\u6355\u83b7\u533a\u57df\uff08capture area\uff09\uff0c\u6355\u83b7\u533a\u57df\u662f\u4e00\u4e2a6.5*6.5mm \u7684\u6b63\u65b9\u5f62\u533a\u57df\uff0c\u6bcf\u4e2a\u6355\u83b7\u533a\u57df\u5305\u542b\u7ea6 5000 \u4e2a spot\uff0c\u6bcf\u4e2a spot \u4e0a\u7ed3\u5408\u6709\u6355\u83b7\u5e8f\u5217\uff0c\u6355\u83b7\u5e8f\u5217\u6709barcode\u5e8f\u5217\u8bb0\u5f55spot \u4f4d\u7f6e\u4fe1\u606f\u548c poly(dT)\u5e8f\u5217\u7ed3\u5408 mRNA\u7684poly(A)\u5c3e\uff0c\u5728\u6355\u83b7mRNA \u7684\u540c\u65f6\u4e5f\u4fdd\u7559\u4e86 mRNA \u7684\u4f4d\u7f6e\u4fe1\u606f\u3002\u8f6c\u5f55\u672c\u7684\u4f4d\u7f6e\u4fe1\u606f\u7ed3\u5408\u67d3\u8272\u62cd\u7167\u7ed3\u679c\u5373\u53ef\u8fd8\u539f\u8f6c\u5f55\u672c\u5728\u7ec4\u7ec7\u7684\u4f4d\u7f6e\u5206\u5e03\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.40555555555555556\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_uRMf0poACDRjZBvL6BtvPicQ9FGoqg-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYNIV9KjUMmsficwAWic7MOBdSBbsuRMf0poACDRjZBvL6BtvPicQ9FGoqg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>\u76ee\u524d\u80fd\u505a\u7684\u4e3b\u8981\u8fd8\u662f\u4eba\u7c7b\u548c\u8001\u9f20\uff0c\u5176\u4ed6\u7684\u8fd8\u4e0d\u80fd\u505a\u3002<\/p>\n<p><strong>\u603b\u4f53\u5de5\u4f5c\u6d41\u7a0b<\/strong><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.2\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_rcUU1a4cLSvND4ku2d031EechkiaFg-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYAicQoFZibVicvHh95iaz1Bwl3R9LYrcUU1a4cLSvND4ku2d031EechkiaFg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>\u9996\u5148\u9009\u62e9\u7ec4\u7ec7\u4e0e\u5207\u7247\uff0c\u5c06\u5207\u7247\u653e\u7f6e\u5728\u73bb\u7247\u4e0a\uff0c\u5269\u4e0b\u7684\u5efa\u5e93\u6d4b\u5e8f\u5c31\u548c\u4e8c\u4ee3\u6d4b\u5e8f\u4e00\u81f4\u4e86\u3002<\/p>\n<p><br \/><\/p>\n<p>\u65b0\u9c9c\u51b7\u51bb\uff08FF\uff09\u7ec4\u7ec7\u7684\u7a7a\u95f4\u57fa\u56e0\u8868\u8fbe\u662f\u4f7f\u7528spaceranger\u8ba1\u6570\u8f6f\u4ef6\u6765\u786e\u5b9a\u7684\uff0c\u8be5\u8f6f\u4ef6\u4ee5\u89c6\u89c9\u8f7d\u73bb\u7247\u7684\u663e\u5fae\u955c\u56fe\u50cf\uff08TIFF\u6216JPEG\u683c\u5f0f\uff09\u548c\u6837\u672cFASTQ\u6587\u4ef6\u4f5c\u4e3a\u8f93\u5165\u3002\u8f6f\u4ef6\u6267\u884c\u5bf9\u9f50\u3001\u7ec4\u7ec7\u548c\u57fa\u51c6\u68c0\u6d4b\u4ee5\u53ca\u6761\u5f62\u7801\/UMI\u8ba1\u6570\u3002\u8f93\u51fa\u6355\u83b7\u7279\u5f81\u70b9\u77e9\u9635\u3001\u805a\u7c7b\u548c\u5dee\u5f02\u57fa\u56e0\u8868\u8fbe\uff08DGE\uff09\uff0c\u8fd9\u4e9b\u53ef\u4ee5\u901a\u8fc7\u5728Loupe Browser\u4e2d\u8fdb\u884c\u8fdb\u4e00\u6b65\u5206\u6790\u548c\u53ef\u89c6\u5316\u3002<\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin: 20px 0%;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(204, 223, 237);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);padding-right: 10px;align-self: flex-start;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(60, 86, 125);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);align-self: flex-start;\">\n<section style=\"color: rgb(255, 255, 254);line-height: 2;padding-right: 15px;padding-left: 15px;text-align: justify;\" powered-by=\"xiumi.us\">\n<p><strong>1.\u51c6\u5907\u5de5\u4f5c<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">curl -o spaceranger-<span class=\"code-snippet__number\">2.1<\/span>.0.tar.xz <span class=\"code-snippet__string\">\"https:\/\/cf.10xgenomics.com\/releases\/spatial-exp\/spaceranger-2.1.0.tar.xz?Expires=1691721798&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=nKggndnkZPv4IEuizxHUM5KSXSbtMV7Tk1ICeslkhK09CuFDeOkPBJQcY1yti7s2fMz8i9b2lySWmBeoFdXMS23~ioITj-L88h0ldpTo7G~pqKvMgIssE5b67C4XZ3FprDdceGIaatl4nt~Vrjg~31FZcbG-JxJAQLFf92XLT1tSfSuovhTb5lFkY3oLTfR6Ozvo7Hbx6bibCqSh4MrEefajch4OvdKIlR0l3svzxX0ExETxBiKJw6PBZJu5qYX6vMtDz2anb0-N4-cbhv5Opl3NlT8Xd-7v3mKDCLN37zM-m4vO9EF6YWG2sBljc7ehxsWQnMWw60VhvtbKVb9ULw__\"<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.8148148148148148\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_lLVAsbHicbIpBCLZPN330ZVjPqoNgg-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYcRUJuL6k2hcOUmr2a7zIt7AwiclLVAsbHicbIpBCLZPN330ZVjPqoNgg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u8f93\u51fa\u8f6f\u4ef6\u7684\u5e2e\u52a9\u6587\u6863\uff0c\u5373\u4e3a\u5b89\u88c5\u6210\u529f\u3002<br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>\u6570\u636e\u96c6\u4fe1\u606f<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>\u5927\u6d77\u54e5\u5728\u672c\u6b21\u6559\u7a0b\u4e2d\u4f7f\u7528\u7684\u662f\u5c0f\u9f20\u5927\u8111\u51a0\u72b6\u5207\u7247\u6570\u636e\u96c6<\/p>\n<p>\u6570\u636e\u96c6\u7684\u4e3b\u8981\u4fe1\u606f\u5305\u62ec\uff1a<\/p>\n<p>10\u00b5m\u539a\u7684\u7ec4\u7ec7\u5207\u7247<\/p>\n<p>\u4f7f\u7528Nikon Ti2-E\u663e\u5fae\u955c\u83b7\u53d6\u7684H&E\u56fe\u50cf<\/p>\n<p>\u6d4b\u5e8f\u6df1\u5ea6\uff1a\u6bcf\u4e2a\u70b9115569\u4e2a\u8bfb\u53d6\u5bf9<\/p>\n<p>\u6d4b\u5e8f\u8986\u76d6\u8303\u56f4\uff1areads 1-28bp\uff08\u5305\u62ec16bp\u7684\u7a7a\u95f4\u6761\u5f62\u7801\uff0c12bp\u7684UMI\uff09\uff1breads 2-120bp\uff08\u8f6c\u5f55\u672c\uff09\uff1bi7\u6837\u672c\u6307\u6570-10bp\uff1bi5\u6837\u672c\u6307\u6570-10 bp<\/p>\n<p>Visium\u5e7b\u706f\u7247\uff1aV19L01-041<\/p>\n<p>\u6355\u83b7\u533a\u57df\uff1aC1<\/p>\n<p>\u793a\u4f8b\u6570\u636e\u96c6\u5305\u62ec\u5177\u6709\u57fa\u51c6\u5e27\u6e05\u6670\u89c6\u56fe\u7684\u660e\u573a\u56fe\u50cf\uff0c\u4ece\u800c\u80fd\u591f\u4f7f\u7528spaceranger\u81ea\u52a8\u56fe\u50cf\u5904\u7406\u6d41\u6c34\u7ebf\u3002<\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>\u4e0b\u8f7d\u6570\u636e\u96c6\u4e0e\u53c2\u8003\u5e8f\u5217<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Create datasets folder<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">mkdir datasets<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Download FASTQ to datasets folder<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">curl https:\/\/s3-us-west-<span class=\"code-snippet__number\">2.<\/span>amazonaws.com\/<span class=\"code-snippet__number\">10<\/span>x.files\/samples\/spatial-<span class=\"code-snippet__built_in\">exp<\/span>\/<span class=\"code-snippet__number\">1.1<\/span>.0\/V1_Adult_Mouse_Brain\/V1_Adult_Mouse_Brain_fastqs.tar -o datasets\/V1_Adult_Mouse_Brain_fastqs.tar<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Download image file to datasets folder<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">curl https:\/\/cf.10xgenomics.com\/samples\/spatial-<span class=\"code-snippet__built_in\">exp<\/span>\/<span class=\"code-snippet__number\">1.1<\/span>.0\/V1_Adult_Mouse_Brain\/V1_Adult_Mouse_Brain_image.tif -o datasets\/V1_Adult_Mouse_Brain_image.tif<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Download mouse reference<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">curl -O https:\/\/cf.10xgenomics.com\/supp\/spatial-<span class=\"code-snippet__built_in\">exp<\/span>\/refdata-gex-mm10-<span class=\"code-snippet__number\">2020<\/span>-A.tar.gz<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>\u89e3\u538b\u7f29\u6570\u636e\u96c6\u4e0e\u53c2\u8003\u5e8f\u5217<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u89e3\u538b\u7f29\u6d4b\u5e8f\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">tar -xvf V1_Adult_Mouse_Brain_fastqs.tar -C .\/ && rm V1_Adult_Mouse_Brain_fastqs.tar<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Extract mouse reference transcriptome<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">tar -xzvf refdata-gex-mm10-<span class=\"code-snippet__number\">2020<\/span>-A.tar.gz && rm refdata-gex-mm10-<span class=\"code-snippet__number\">2020<\/span>-A.tar.gz<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3844660194174757\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_e5vPJNXBI60Q6NxgduuD4YtkR6orIA-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYV6SjLo4svRHViaaoibMRC0EryRe5vPJNXBI60Q6NxgduuD4YtkR6orIA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1030\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u89e3\u538b\u6210\u529f\u540e\u5c06\u4f1a\u5305\u542b\u4ee5\u4e0a\u6587\u4ef6<\/p>\n<ol style=\"list-style-type: chineseCounting;padding-left: 40px;list-style-position: outside;\" powered-by=\"xiumi.us\" class=\"list-paddingleft-1\">\n<li>\n<p>spaceranger\u5206\u6790<\/p>\n<\/li>\n<\/ol>\n<p powered-by=\"xiumi.us\">\u73b0\u5728\u6211\u4eec\u6240\u6709\u7684\u8f93\u5165\u6587\u4ef6(\u53c2\u8003\u57fa\u56e0\uff0c\u6d4b\u5e8f\u6587\u4ef6\uff0c\u5207\u7247\u7167\u7247)\u90fd\u51c6\u5907\u597d\u4e86\uff0c\u5c31\u53ef\u4ee5\u4f7f\u7528spaceranger\u6765\u8fdb\u884c\u5206\u6790\u3002<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">spaceranger count --id=<span class=\"code-snippet__string\">\"V1_Adult_Mouse_Brain\"<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"> --description=<span class=\"code-snippet__string\">\"Adult Mouse Brain (Coronal)\"<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"> --transcriptome=refdata-gex-mm10-<span class=\"code-snippet__number\">2020<\/span>-A <\/span><\/code><code><span class=\"code-snippet_outer\"> --fastqs=V1_Adult_Mouse_Brain_fastqs <\/span><\/code><code><span class=\"code-snippet_outer\"> --image=V1_Adult_Mouse_Brain_image.tif <\/span><\/code><code><span class=\"code-snippet_outer\"> --slide=V19L01-<span class=\"code-snippet__number\">041<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"> --area=C1 <\/span><\/code><code><span class=\"code-snippet_outer\"> --localcores=<span class=\"code-snippet__number\">16<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"> --localmem=<span class=\"code-snippet__number\">128<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.45698166431593795\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_JySvGnq4thRGRWYFiclpOpZuWKaM8Q-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYqVhCSksVnTMLicbnRdBEqFdNjBJySvGnq4thRGRWYFiclpOpZuWKaM8Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"709\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">spaceranger\u53c2\u6570\u542b\u4e49<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5444444444444444\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_DzBGxmiaoVX34Djn3JWtsZc9FvyzRQ-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyY4uaOjjU6TrNT0m94a5eMRCZib3DzBGxmiaoVX34Djn3JWtsZc9FvyzRQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>\u8fd0\u884c\u597d\u540e\u5c06\u4f1a\u770b\u5230\u4ee5\u4e0a\u8f93\u51fa\u3002<\/p>\n<p><br 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238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>outs\u4e0b\u7684\u8f93\u51fa\u6587\u4ef6<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.8892339544513458\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_dSkwrM0xWIcpKoNvGaEJ5wcVh5EvYg-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYQztKoJEN1a0dQBGKpAx6Rp8YdSkwrM0xWIcpKoNvGaEJ5wcVh5EvYg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"966\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 2em;text-wrap: wrap;\"><br \/><\/p>\n<p style=\"text-indent: 2em;text-wrap: wrap;\">\u4ece\u4e0a\u81f3\u4e0b\u4f9d\u6b21\u662f\uff0c\u5206\u6790\u603b\u7ed3\u7ed3\u679c\uff0cloupebrowser\u5206\u6790\u7684\u6587\u4ef6\uff0c\u4e8c\u7ea7\u5206\u6790\u7ed3\u679c\uff08\u805a\u7c7b\u3001DGE\u3001Moran\u2019 s I\uff09\uff0c\u57fa\u51c6\u5bf9\u51c6\u3001\u4f4e\u5206\u8fa8\u7387\u548c\u9ad8\u5206\u8fa8\u7387\u56fe\u50cf\u4ee5\u53ca\u5149\u6591\u6bd4\u5bf9\u7ed3\u679c\uff0cbam\u6bd4\u5bf9\u6587\u4ef6,\u4e0b\u6e38\u5206\u6790\u7684\u6587\u4ef6\u3002<\/p>\n<\/section>\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"box-shadow: rgb(170, 170, 170) 0px 0px 10px inset;border-color: rgb(192, 200, 209);padding: 10px;border-width: 1px;border-style: solid;background-color: rgb(250, 250, 239);\">\n<section style=\"color: rgb(51, 51, 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powered-by=\"xiumi.us\"><br \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><span style=\"font-family: mp-quote, -apple-system-font, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;\">\u4eca\u5929\u5927\u6d77<\/span><span style=\"font-family: mp-quote, -apple-system-font, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;\">\u54e5\u5e26\u5927\u5bb6\u4f7f\u7528spaceranger\u8fdb\u884c\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u7684\u5206\u6790\u3002<\/span><br \/><\/p>\n<p powered-by=\"xiumi.us\"><span style=\"font-family: mp-quote, -apple-system-font, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;\"><br \/><\/span><\/p>\n<section powered-by=\"xiumi.us\">\n<p><strong>\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u7b80\u4ecb<\/strong><\/p>\n<p>\u4f20\u7edf\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\uff0c\u662f\u5c06\u7ec6\u80de\u7ec4\u7ec7\u5236\u5907\u6210\u7ec6\u80de\u60ac\u6d4a\u6db2\uff0c\u8fd9\u6837\u4e00\u6765\u5c31\u4e22\u5931\u4e86\u7ec6\u80de\u6240\u5904\u4f4d\u7f6e\u7684\u7a7a\u95f4\u4fe1\u606f\u3002\u7a7a\u95f4\u8f6c\u5f55\u7ec4\uff08spatial gene expression\uff09, \u7b80\u79f0ST-seq, \u5728\u6b64\u57fa\u7840\u4e4b\u4e0a\u5c06\u8f6c\u5f55\u7ec4\u5b66\uff0c\u5355\u7ec6\u80de\u6d4b\u5e8f\u6280\u672f\u4ee5\u53ca\u7ec4\u7ec7\u5207\u7247\u6280\u672f\u7ed3\u5408\u8d77\u6765\u7684\u6280\u672f\u3002\u800c\u7ec4\u7ec7\u5207\u7247\u5c06\u63d0\u4f9b\u7ec6\u80de\u7684\u7a7a\u95f4\u4fe1\u606f\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.4787037037037037\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_IoNdd2HicoT9zkO7hA93skwY9rxVDg-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYicCDZicoI5eibk0ibO86WM0WsWYwqIoNdd2HicoT9zkO7hA93skwY9rxVDg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>\u901a\u8fc7\u8fdb\u884c\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\uff0c\u5c31\u80fd\u77e5\u9053\u4e00\u7247\u7ec4\u7ec7\u7684\u7ec6\u80de\u7c7b\u578b\uff0c\u53ca\u5176\u4f4d\u7f6e\uff0c\u8fd9\u5bf9\u7814\u7a76\u80bf\u7624\u7ec4\u7ec7\u4f1a\u6709\u5f88\u5927\u7684\u5e2e\u52a9\u3002<\/p>\n<p>10\u00d7genomics Visium \u7a7a\u95f4\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\u5e73\u53f0<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3388888888888889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_3DsibSQ9cXc0a8lzQYWlZ67o4WJjlg-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYAw9fubNp7XpwogkdsObiak4tof3DsibSQ9cXc0a8lzQYWlZ67o4WJjlg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">10x Visium \u4f7f\u7528\u7279\u5236\u7684\u73bb\u7247\uff0c\u73bb\u7247\u4e0a\u6709\u6355\u83b7\u533a\u57df\uff08capture area\uff09\uff0c\u6355\u83b7\u533a\u57df\u662f\u4e00\u4e2a6.5*6.5mm \u7684\u6b63\u65b9\u5f62\u533a\u57df\uff0c\u6bcf\u4e2a\u6355\u83b7\u533a\u57df\u5305\u542b\u7ea6 5000 \u4e2a spot\uff0c\u6bcf\u4e2a spot \u4e0a\u7ed3\u5408\u6709\u6355\u83b7\u5e8f\u5217\uff0c\u6355\u83b7\u5e8f\u5217\u6709barcode\u5e8f\u5217\u8bb0\u5f55spot \u4f4d\u7f6e\u4fe1\u606f\u548c poly(dT)\u5e8f\u5217\u7ed3\u5408 mRNA\u7684poly(A)\u5c3e\uff0c\u5728\u6355\u83b7mRNA \u7684\u540c\u65f6\u4e5f\u4fdd\u7559\u4e86 mRNA \u7684\u4f4d\u7f6e\u4fe1\u606f\u3002\u8f6c\u5f55\u672c\u7684\u4f4d\u7f6e\u4fe1\u606f\u7ed3\u5408\u67d3\u8272\u62cd\u7167\u7ed3\u679c\u5373\u53ef\u8fd8\u539f\u8f6c\u5f55\u672c\u5728\u7ec4\u7ec7\u7684\u4f4d\u7f6e\u5206\u5e03\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.40555555555555556\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_uRMf0poACDRjZBvL6BtvPicQ9FGoqg-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYNIV9KjUMmsficwAWic7MOBdSBbsuRMf0poACDRjZBvL6BtvPicQ9FGoqg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>\u76ee\u524d\u80fd\u505a\u7684\u4e3b\u8981\u8fd8\u662f\u4eba\u7c7b\u548c\u8001\u9f20\uff0c\u5176\u4ed6\u7684\u8fd8\u4e0d\u80fd\u505a\u3002<\/p>\n<p><strong>\u603b\u4f53\u5de5\u4f5c\u6d41\u7a0b<\/strong><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.2\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_rcUU1a4cLSvND4ku2d031EechkiaFg-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYAicQoFZibVicvHh95iaz1Bwl3R9LYrcUU1a4cLSvND4ku2d031EechkiaFg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>\u9996\u5148\u9009\u62e9\u7ec4\u7ec7\u4e0e\u5207\u7247\uff0c\u5c06\u5207\u7247\u653e\u7f6e\u5728\u73bb\u7247\u4e0a\uff0c\u5269\u4e0b\u7684\u5efa\u5e93\u6d4b\u5e8f\u5c31\u548c\u4e8c\u4ee3\u6d4b\u5e8f\u4e00\u81f4\u4e86\u3002<\/p>\n<p><br \/><\/p>\n<p>\u65b0\u9c9c\u51b7\u51bb\uff08FF\uff09\u7ec4\u7ec7\u7684\u7a7a\u95f4\u57fa\u56e0\u8868\u8fbe\u662f\u4f7f\u7528spaceranger\u8ba1\u6570\u8f6f\u4ef6\u6765\u786e\u5b9a\u7684\uff0c\u8be5\u8f6f\u4ef6\u4ee5\u89c6\u89c9\u8f7d\u73bb\u7247\u7684\u663e\u5fae\u955c\u56fe\u50cf\uff08TIFF\u6216JPEG\u683c\u5f0f\uff09\u548c\u6837\u672cFASTQ\u6587\u4ef6\u4f5c\u4e3a\u8f93\u5165\u3002\u8f6f\u4ef6\u6267\u884c\u5bf9\u9f50\u3001\u7ec4\u7ec7\u548c\u57fa\u51c6\u68c0\u6d4b\u4ee5\u53ca\u6761\u5f62\u7801\/UMI\u8ba1\u6570\u3002\u8f93\u51fa\u6355\u83b7\u7279\u5f81\u70b9\u77e9\u9635\u3001\u805a\u7c7b\u548c\u5dee\u5f02\u57fa\u56e0\u8868\u8fbe\uff08DGE\uff09\uff0c\u8fd9\u4e9b\u53ef\u4ee5\u901a\u8fc7\u5728Loupe Browser\u4e2d\u8fdb\u884c\u8fdb\u4e00\u6b65\u5206\u6790\u548c\u53ef\u89c6\u5316\u3002<\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin: 20px 0%;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(204, 223, 237);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);padding-right: 10px;align-self: flex-start;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(60, 86, 125);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);align-self: flex-start;\">\n<section style=\"color: rgb(255, 255, 254);line-height: 2;padding-right: 15px;padding-left: 15px;text-align: justify;\" powered-by=\"xiumi.us\">\n<p><strong>1.\u51c6\u5907\u5de5\u4f5c<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">curl -o spaceranger-<span class=\"code-snippet__number\">2.1<\/span>.0.tar.xz <span class=\"code-snippet__string\">\"https:\/\/cf.10xgenomics.com\/releases\/spatial-exp\/spaceranger-2.1.0.tar.xz?Expires=1691721798&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=nKggndnkZPv4IEuizxHUM5KSXSbtMV7Tk1ICeslkhK09CuFDeOkPBJQcY1yti7s2fMz8i9b2lySWmBeoFdXMS23~ioITj-L88h0ldpTo7G~pqKvMgIssE5b67C4XZ3FprDdceGIaatl4nt~Vrjg~31FZcbG-JxJAQLFf92XLT1tSfSuovhTb5lFkY3oLTfR6Ozvo7Hbx6bibCqSh4MrEefajch4OvdKIlR0l3svzxX0ExETxBiKJw6PBZJu5qYX6vMtDz2anb0-N4-cbhv5Opl3NlT8Xd-7v3mKDCLN37zM-m4vO9EF6YWG2sBljc7ehxsWQnMWw60VhvtbKVb9ULw__\"<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.8148148148148148\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_lLVAsbHicbIpBCLZPN330ZVjPqoNgg-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYcRUJuL6k2hcOUmr2a7zIt7AwiclLVAsbHicbIpBCLZPN330ZVjPqoNgg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u8f93\u51fa\u8f6f\u4ef6\u7684\u5e2e\u52a9\u6587\u6863\uff0c\u5373\u4e3a\u5b89\u88c5\u6210\u529f\u3002<br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>\u6570\u636e\u96c6\u4fe1\u606f<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>\u5927\u6d77\u54e5\u5728\u672c\u6b21\u6559\u7a0b\u4e2d\u4f7f\u7528\u7684\u662f\u5c0f\u9f20\u5927\u8111\u51a0\u72b6\u5207\u7247\u6570\u636e\u96c6<\/p>\n<p>\u6570\u636e\u96c6\u7684\u4e3b\u8981\u4fe1\u606f\u5305\u62ec\uff1a<\/p>\n<p>10\u00b5m\u539a\u7684\u7ec4\u7ec7\u5207\u7247<\/p>\n<p>\u4f7f\u7528Nikon Ti2-E\u663e\u5fae\u955c\u83b7\u53d6\u7684H&E\u56fe\u50cf<\/p>\n<p>\u6d4b\u5e8f\u6df1\u5ea6\uff1a\u6bcf\u4e2a\u70b9115569\u4e2a\u8bfb\u53d6\u5bf9<\/p>\n<p>\u6d4b\u5e8f\u8986\u76d6\u8303\u56f4\uff1areads 1-28bp\uff08\u5305\u62ec16bp\u7684\u7a7a\u95f4\u6761\u5f62\u7801\uff0c12bp\u7684UMI\uff09\uff1breads 2-120bp\uff08\u8f6c\u5f55\u672c\uff09\uff1bi7\u6837\u672c\u6307\u6570-10bp\uff1bi5\u6837\u672c\u6307\u6570-10 bp<\/p>\n<p>Visium\u5e7b\u706f\u7247\uff1aV19L01-041<\/p>\n<p>\u6355\u83b7\u533a\u57df\uff1aC1<\/p>\n<p>\u793a\u4f8b\u6570\u636e\u96c6\u5305\u62ec\u5177\u6709\u57fa\u51c6\u5e27\u6e05\u6670\u89c6\u56fe\u7684\u660e\u573a\u56fe\u50cf\uff0c\u4ece\u800c\u80fd\u591f\u4f7f\u7528spaceranger\u81ea\u52a8\u56fe\u50cf\u5904\u7406\u6d41\u6c34\u7ebf\u3002<\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>\u4e0b\u8f7d\u6570\u636e\u96c6\u4e0e\u53c2\u8003\u5e8f\u5217<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Create datasets folder<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">mkdir datasets<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Download FASTQ to datasets folder<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">curl https:\/\/s3-us-west-<span class=\"code-snippet__number\">2.<\/span>amazonaws.com\/<span class=\"code-snippet__number\">10<\/span>x.files\/samples\/spatial-<span class=\"code-snippet__built_in\">exp<\/span>\/<span class=\"code-snippet__number\">1.1<\/span>.0\/V1_Adult_Mouse_Brain\/V1_Adult_Mouse_Brain_fastqs.tar -o datasets\/V1_Adult_Mouse_Brain_fastqs.tar<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Download image file to datasets folder<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">curl https:\/\/cf.10xgenomics.com\/samples\/spatial-<span class=\"code-snippet__built_in\">exp<\/span>\/<span class=\"code-snippet__number\">1.1<\/span>.0\/V1_Adult_Mouse_Brain\/V1_Adult_Mouse_Brain_image.tif -o datasets\/V1_Adult_Mouse_Brain_image.tif<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Download mouse reference<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">curl -O https:\/\/cf.10xgenomics.com\/supp\/spatial-<span class=\"code-snippet__built_in\">exp<\/span>\/refdata-gex-mm10-<span class=\"code-snippet__number\">2020<\/span>-A.tar.gz<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>\u89e3\u538b\u7f29\u6570\u636e\u96c6\u4e0e\u53c2\u8003\u5e8f\u5217<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u89e3\u538b\u7f29\u6d4b\u5e8f\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">tar -xvf V1_Adult_Mouse_Brain_fastqs.tar -C .\/ && rm V1_Adult_Mouse_Brain_fastqs.tar<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Extract mouse reference transcriptome<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">tar -xzvf refdata-gex-mm10-<span class=\"code-snippet__number\">2020<\/span>-A.tar.gz && rm refdata-gex-mm10-<span class=\"code-snippet__number\">2020<\/span>-A.tar.gz<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3844660194174757\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_e5vPJNXBI60Q6NxgduuD4YtkR6orIA-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYV6SjLo4svRHViaaoibMRC0EryRe5vPJNXBI60Q6NxgduuD4YtkR6orIA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1030\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u89e3\u538b\u6210\u529f\u540e\u5c06\u4f1a\u5305\u542b\u4ee5\u4e0a\u6587\u4ef6<\/p>\n<ol style=\"list-style-type: chineseCounting;padding-left: 40px;list-style-position: outside;\" powered-by=\"xiumi.us\" class=\"list-paddingleft-1\">\n<li>\n<p>spaceranger\u5206\u6790<\/p>\n<\/li>\n<\/ol>\n<p powered-by=\"xiumi.us\">\u73b0\u5728\u6211\u4eec\u6240\u6709\u7684\u8f93\u5165\u6587\u4ef6(\u53c2\u8003\u57fa\u56e0\uff0c\u6d4b\u5e8f\u6587\u4ef6\uff0c\u5207\u7247\u7167\u7247)\u90fd\u51c6\u5907\u597d\u4e86\uff0c\u5c31\u53ef\u4ee5\u4f7f\u7528spaceranger\u6765\u8fdb\u884c\u5206\u6790\u3002<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">spaceranger count --id=<span class=\"code-snippet__string\">\"V1_Adult_Mouse_Brain\"<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"> --description=<span class=\"code-snippet__string\">\"Adult Mouse Brain (Coronal)\"<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"> --transcriptome=refdata-gex-mm10-<span class=\"code-snippet__number\">2020<\/span>-A <\/span><\/code><code><span class=\"code-snippet_outer\"> --fastqs=V1_Adult_Mouse_Brain_fastqs <\/span><\/code><code><span class=\"code-snippet_outer\"> --image=V1_Adult_Mouse_Brain_image.tif <\/span><\/code><code><span class=\"code-snippet_outer\"> --slide=V19L01-<span class=\"code-snippet__number\">041<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"> --area=C1 <\/span><\/code><code><span class=\"code-snippet_outer\"> --localcores=<span class=\"code-snippet__number\">16<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"> --localmem=<span class=\"code-snippet__number\">128<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.45698166431593795\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_JySvGnq4thRGRWYFiclpOpZuWKaM8Q-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYqVhCSksVnTMLicbnRdBEqFdNjBJySvGnq4thRGRWYFiclpOpZuWKaM8Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"709\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">spaceranger\u53c2\u6570\u542b\u4e49<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5444444444444444\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_DzBGxmiaoVX34Djn3JWtsZc9FvyzRQ-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyY4uaOjjU6TrNT0m94a5eMRCZib3DzBGxmiaoVX34Djn3JWtsZc9FvyzRQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>\u8fd0\u884c\u597d\u540e\u5c06\u4f1a\u770b\u5230\u4ee5\u4e0a\u8f93\u51fa\u3002<\/p>\n<p><br \/><\/p>\n<p>\u8f93\u51fa\u7684\u6587\u4ef6\u5305\u62ec<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9808219178082191\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_QvAmnWaj18pwOfricWjrWThcu63MvA-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYownpJwHveAXd0BFhe0LvXPE6VQvAmnWaj18pwOfricWjrWThcu63MvA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"365\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<ul style=\"list-style-type: \uf0b7;padding-left: 40px;list-style-position: outside;\" class=\"list-paddingleft-1\" powered-by=\"xiumi.us\">\n<li>\n<p>outs\u5305\u542b\u6240\u6709\u6700\u7ec8\u751f\u6210\u7684\u8f93\u51fa<\/p>\n<\/li>\n<li>\n<p>V1_Adult_Mouse_Brain.mri.tgz\u5305\u542b\u6709\u52a9\u4e8e 10x \u57fa\u56e0\u7ec4\u5b66\u652f\u6301\u89e3\u51b3\u4efb\u4f55\u9519\u8bef\u7684\u8bca\u65ad\u4fe1\u606f<\/p>\n<\/li>\n<li>\n<p>_sitecheck\u6355\u83b7\u7c7b\u4f3c\u4e8e SiteCheck \u5b50\u547d\u4ee4\u7684\u7cfb\u7edf\u914d\u7f6e<\/p>\n<\/li>\n<li>\n<p>_timestamp\u5305\u542b\u6709\u5173\u8fd0\u884c\u65f6\u7684\u4fe1\u606f<\/p>\n<\/li>\n<li>\n<p>_cmdline\u6355\u83b7\u4e3a\u8fd0\u884c\u800c\u63d0\u4f9b\u7684\u547d\u4ee4count<\/p>\n<\/li>\n<li>\n<p>_versions\u5305\u542b\u8fd0\u884c\u4e2d\u4f7f\u7528\u7684\u8f6f\u4ef6\u7248\u672c<\/p>\n<\/li>\n<\/ul>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>outs\u4e0b\u7684\u8f93\u51fa\u6587\u4ef6<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.8892339544513458\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_dSkwrM0xWIcpKoNvGaEJ5wcVh5EvYg-3.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYQztKoJEN1a0dQBGKpAx6Rp8YdSkwrM0xWIcpKoNvGaEJ5wcVh5EvYg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"966\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 2em;text-wrap: wrap;\"><br \/><\/p>\n<p style=\"text-indent: 2em;text-wrap: wrap;\">\u4ece\u4e0a\u81f3\u4e0b\u4f9d\u6b21\u662f\uff0c\u5206\u6790\u603b\u7ed3\u7ed3\u679c\uff0cloupebrowser\u5206\u6790\u7684\u6587\u4ef6\uff0c\u4e8c\u7ea7\u5206\u6790\u7ed3\u679c\uff08\u805a\u7c7b\u3001DGE\u3001Moran\u2019 s I\uff09\uff0c\u57fa\u51c6\u5bf9\u51c6\u3001\u4f4e\u5206\u8fa8\u7387\u548c\u9ad8\u5206\u8fa8\u7387\u56fe\u50cf\u4ee5\u53ca\u5149\u6591\u6bd4\u5bf9\u7ed3\u679c\uff0cbam\u6bd4\u5bf9\u6587\u4ef6,\u4e0b\u6e38\u5206\u6790\u7684\u6587\u4ef6\u3002<\/p>\n<\/section>\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"box-shadow: rgb(170, 170, 170) 0px 0px 10px inset;border-color: rgb(192, 200, 209);padding: 10px;border-width: 1px;border-style: solid;background-color: rgb(250, 250, 239);\">\n<section style=\"color: rgb(51, 51, 51);text-align: center;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p style=\"text-align: justify;text-wrap: wrap;\"><span style=\"font-size: 14px;letter-spacing: 2px;text-wrap: wrap;\">\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u81ea\u5df1\u52a8\u624b\u8bd5\u8bd5\u5427\u3002\u5927\u6d77\u54e5\u4eca\u5929\u7684\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5206\u6790\u8bfe\u5c31\u5230\u8fd9\u91cc\u4e86\u3002\u6211\u4eec\u4e0b\u6b21\u89c1~<\/span><\/p>\n<p style=\"text-align: justify;text-wrap: wrap;\">\uff08\u6700\u540e\u63a8\u8350\u4e00\u4e0b\u5927\u6d77\u54e5\u65b0\u5f00\u53d1\u7684\u96f6\u4ee3\u7801\u4e91\u751f\u4fe1\u5206\u6790\u5de5\u5177\u5e73\u53f0\u5305\u542b\u8d85\u591a\u96f6\u4ee3\u7801\u5c0f\u5de5\u5177\uff0c\u4e0a\u4f20\u6570\u636e\u4e00\u952e\u51fa\u56fe\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u6765\u53c2\u89c2\u54df\u3002<\/p>\n<p style=\"text-align: justify;text-wrap: wrap;\">\u7f51\u5740\uff1a<\/p>\n<p style=\"text-align: justify;text-wrap: wrap;\">http:\/\/www.biocloudservice.com\/home.html\uff09<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: center;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(231, 215, 173);\">\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-left: 10px;padding-right: 10px;\">\n<section style=\"transform: scale(2);transform-origin: center center;margin-top: 50px;margin-bottom: 50px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-left: -6px;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px 3px 0px 0px;border-color: rgb(231, 215, 173) rgb(231, 215, 173) rgb(69, 107, 176) rgb(69, 107, 176);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-left: -6px;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px 3px 0px 0px;border-color: rgb(231, 215, 173) rgb(231, 215, 173) rgb(69, 107, 176) rgb(69, 107, 176);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-left: -6px;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px 3px 0px 0px;border-color: rgb(231, 215, 173) rgb(231, 215, 173) rgb(69, 107, 176) rgb(69, 107, 176);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 38%;align-self: center;flex: 0 0 auto;height: auto;padding: 3px 23px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 100%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.025925925925926\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_NFyts1jxLwtHpMHTxskBcGCRyBZibw-3.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/YHGRXkSxjST4qLG4uyZGiat2dSF4icr7CDJopBdYOsYbuPCJl1vOEz4OR6DNFyts1jxLwtHpMHTxskBcGCRyBZibw\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;transform: rotateY(180deg);\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-left: -6px;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px 3px 0px 0px;border-color: rgb(231, 215, 173) rgb(231, 215, 173) rgb(69, 107, 176) rgb(69, 107, 176);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 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