{"id":55670,"date":"2024-06-16T17:07:15","date_gmt":"2024-06-16T09:07:15","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55670"},"modified":"2024-06-16T17:07:15","modified_gmt":"2024-06-16T09:07:15","slug":"%e5%bc%ba%e5%a4%a7%e7%9a%84bam%e6%96%87%e4%bb%b6%e5%a4%84%e7%90%86%e5%b7%a5%e5%85%b7-sambamba","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55670","title":{"rendered":"\u5f3a\u5927\u7684bam\u6587\u4ef6\u5904\u7406\u5de5\u5177\u2014\u2014sambamba"},"content":{"rendered":"


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id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c  <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-11-16 19:00:34<\/em><\/div>\n<p><content><\/p>\n<p style=\"text-align: center;\" data-mpa-powered-by=\"yiban.io\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.23333333333333334\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_rE2v0O5WiaUDJjgv5bxK2G1ePHyH2g-36.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAysHY49vPGCraibakiaNq6hQgn8C5MfvVLQGxlNZPEia2PajKAzrE2v0O5WiaUDJjgv5bxK2G1ePHyH2g\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<h1 style=\"text-indent: 0em;\"><span style=\"font-size: 15px;\">\u5c0f\u679c\u6700\u8fd1\u5728\u505abam\u6587\u4ef6\u7684\u5904\u7406\uff0csamtoools\u867d\u7136\u529f\u80fd\u5f88\u5f3a\u5927\uff0c\u4f46\u6587\u4ef6\u592a\u5927\u7684\u8bdd\u8fd8\u662f\u5f88\u8017\u65f6\u7684\uff0c\u800c\u4e14\u5728\u7ed9bem\u6587\u4ef6<span style=\"font-family: \u7b49\u7ebf;text-align: left;text-indent: 0em;letter-spacing: 0.034em;\">\u6784\u5efa\u7d22\u5f15\u65f6\u8fd8\u51fa\u73b0\u4e86\u4e0b\u9762\u7684\u9519\u8bef\/(\u3112o\u3112)\/~~<\/span><\/span><\/h1>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"sql\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">$ samtools index -b test.sort.bam test.sort.bam.bai<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">[E::hts_idx_check_range] Region 536870922..536871063 cannot be stored in a bai index. Try using a csi index<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">[E::sam_index] Read 'E00548:269:HV7NVCCXY:3:2117:26494:57301' with ref_name='chr1H', ref_length=558535432, flags=81, pos=536870923 cannot be indexed<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">samtools index: failed to create index for \"Atlas.sort.bam\": Numerical result out of range<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;margin-top: 15.6pt;margin-bottom: 0pt;line-height: 150%;text-indent: 0em;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">\u9519\u8bef\u4fe1\u606f\u8868\u660e\u533a\u57df\u201c<\/span><code style=\"mso-ansi-font-size: 12.0pt;mso-bidi-font-size: 12.0pt;mso-bidi-font-family: \u5b8b\u4f53;font-family: \u5b8b\u4f53;mso-ascii-font-family: \u5b8b\u4f53;mso-fareast-font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: #111111;\"><span style=\"font-size: 15px;\">536870922..536871063<\/span><\/code><span style=\"line-height: 150%;font-family: -apple-system;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">\u201d\u65e0\u6cd5\u5b58\u50a8\u5728\u201c<\/span><code style=\"mso-ansi-font-size: 12.0pt;mso-bidi-font-size: 12.0pt;mso-bidi-font-family: \u5b8b\u4f53;font-family: \u5b8b\u4f53;mso-ascii-font-family: \u5b8b\u4f53;mso-fareast-font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: #111111;\"><span style=\"font-size: 15px;\">.bai<\/span><\/code><span style=\"line-height: 150%;font-family: -apple-system;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">\u201d\u7d22\u5f15\u4e2d\uff0c\u5e76\u5efa\u8bae\u5c1d\u8bd5\u4f7f\u7528\u201c<\/span><code style=\"mso-ansi-font-size: 12.0pt;mso-bidi-font-size: 12.0pt;mso-bidi-font-family: \u5b8b\u4f53;font-family: \u5b8b\u4f53;mso-ascii-font-family: \u5b8b\u4f53;mso-fareast-font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: #111111;\"><span style=\"font-size: 15px;\">.csi<\/span><\/code><span style=\"line-height: 150%;font-family: -apple-system;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">\u201d\u7d22\u5f15\u3002\u8fd9\u662f\u56e0\u4e3a\u201c<\/span><code style=\"mso-ansi-font-size: 12.0pt;mso-bidi-font-size: 12.0pt;mso-bidi-font-family: \u5b8b\u4f53;font-family: \u5b8b\u4f53;mso-ascii-font-family: \u5b8b\u4f53;mso-fareast-font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: #111111;\"><span style=\"font-size: 15px;\">.bai<\/span><\/code><span style=\"line-height: 150%;font-family: -apple-system;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">\u201d\u7d22\u5f15\u683c\u5f0f\u5728\u53ef\u4ee5\u5904\u7406\u7684\u6700\u5927\u53c2\u8003\u5e8f\u5217\u957f\u5ea6\u65b9\u9762\u5b58\u5728\u9650\u5236\uff0c\u5373\u201c<\/span><code style=\"mso-ansi-font-size: 12.0pt;mso-bidi-font-size: 12.0pt;mso-bidi-font-family: \u5b8b\u4f53;font-family: \u5b8b\u4f53;mso-ascii-font-family: \u5b8b\u4f53;mso-fareast-font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: #111111;\"><span style=\"font-size: 15px;\">2<\/span><\/code><code style=\"mso-ansi-font-size: 12.0pt;mso-bidi-font-size: 12.0pt;mso-bidi-font-family: \u5b8b\u4f53;font-family: \u5b8b\u4f53;mso-ascii-font-family: \u5b8b\u4f53;mso-fareast-font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: #111111;\"><span style=\"font-size: 15px;\"><sup>29-1<\/sup><\/span><\/code><span style=\"line-height: 150%;font-family: -apple-system;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">\u201d\u6216<\/span><code style=\"mso-ansi-font-size: 12.0pt;mso-bidi-font-size: 12.0pt;mso-bidi-font-family: \u5b8b\u4f53;font-family: \u5b8b\u4f53;mso-ascii-font-family: \u5b8b\u4f53;mso-fareast-font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: #111111;\"><span style=\"font-size: 15px;\">536870911<\/span><\/code><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">\u3002\u5982\u679c\u53c2\u8003\u5e8f\u5217\u957f\u5ea6\u8d85\u8fc7\u6b64\u503c\uff0c\u5219\u9700\u8981\u4f7f\u7528<\/span><code style=\"mso-ansi-font-size: 12.0pt;mso-bidi-font-size: 12.0pt;mso-bidi-font-family: \u5b8b\u4f53;font-family: \u5b8b\u4f53;mso-ascii-font-family: \u5b8b\u4f53;mso-fareast-font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: #111111;\"><span style=\"font-size: 15px;\">.csi<\/span><\/code><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">\u7d22\u5f15\u683c\u5f0f\u3002<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.38981481481481484\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_IWicymEetCc6Enf2BkUE4Yz1Ya5e5w-20.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyuYsqlJnQUJvnS2iciaDibtfbdV0VmHlriaSjYwjFPLp1BLjokHIWicymEetCc6Enf2BkUE4Yz1Ya5e5w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"letter-spacing: 0.578px;text-align: center;white-space: normal;\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;margin-top: 15.6pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u4f46\u662f\uff01\u4f46\u662fGATK\u7b49\u4e00\u4e9b\u751f\u4fe1\u5de5\u5177\u4f3c\u4e4e\u4e0d\u652f\u6301\u201c.csi\u201d\u683c\u5f0f\u7684\u7d22\u5f15\u6587\u4ef6\uff0c\u600e\u4e48\u529e\uff01(;\u00b4\u0f0e\u0eb6\u0414\u0f0e\u0eb6`)<\/span><\/span><span style=\"font-size: 15px;text-indent: 0em;letter-spacing: 0.034em;font-family: \u7b49\u7ebf;font-variant-numeric: normal;font-variant-east-asian: normal;\">\u8fd8\u597d\uff0c\u5c0f\u679c\u627e\u5230\u4e86sambamba\uff0c\u5b83\u4e0d\u4ec5\u80fd\u591f\u7ed9<\/span><span style=\"font-size: 15px;text-indent: 0em;letter-spacing: 0.034em;font-family: \u7b49\u7ebf;font-variant-numeric: normal;font-variant-east-asian: normal;color: rgb(17, 17, 17);\">\u6700\u5927\u53c2\u8003\u5e8f\u5217\u957f\u5ea6\u7684bam\u6587\u4ef6\u6784\u5efabai\u7d22\u5f15\uff0c\u800c\u4e14<\/span><span style=\"font-size: 15px;text-indent: 0em;letter-spacing: 0.034em;font-family: \u7b49\u7ebf;font-variant-numeric: normal;font-variant-east-asian: normal;\">\u5b83\u8fd8\u80fd\u5feb\u901f\u3001\u9ad8\u6548\u3001\u51c6\u786e\u5730\u5206\u6790\u4f60\u7684BAM\u6587\u4ef6\uff0c\u5b83\u80fd\u5229\u7528\u591a\u6838\u5904\u7406\u5668\uff0c\u8fd9\u4e5f\u5c31\u610f\u5473\u7740\u5927\u5927\u7684\u63d0\u9ad8\u4e86\u6587\u4ef6\u5904\u7406\u6548\u7387\u3002<\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;margin-top: 15.6pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-size: 15px;font-weight: bold;text-indent: 0em;letter-spacing: 0.034em;\">sambamba\u662f\u4ec0\u4e48\uff1f<\/span><br \/><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;margin-top: 15.6pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">sambamba\u662f\u4e00\u4e2a\u65b0\u7684BAM\u6587\u4ef6\u5904\u7406\u5de5\u5177\uff0c\u5b83\u4f7f\u7528\u4e86D\u8bed\u8a00\u7684\u591a\u7ebf\u7a0b\u548c\u5f02\u6b65IO\u7279\u6027\uff0c\u5b9e\u73b0\u4e86\u9ad8\u6548\u7684\u5e76\u884c\u5316\u5904\u7406\u3002sambamba\u53ef\u4ee5\u5728\u591a\u6838CPU\u4e0a\u540c\u65f6\u8fd0\u884c\u591a\u4e2a\u4efb\u52a1\uff0c\u5229\u7528\u786c\u76d8\u548c\u5185\u5b58\u7684\u5e26\u5bbd\uff0c\u63d0\u9ad8\u4e86\u5904\u7406\u901f\u5ea6\u3002sambamba\u8fd8\u4f7f\u7528\u4e86\u4e00\u4e9b\u4f18\u5316\u7b97\u6cd5\u548c\u6570\u636e\u7ed3\u6784\uff0c\u6bd4\u5982\u5feb\u901f\u6392\u5e8f\uff0c\u54c8\u5e0c\u8868\uff0c\u4f4d\u56fe\u7b49\uff0c\u51cf\u5c11\u4e86\u5185\u5b58\u5360\u7528\u548c\u78c1\u76d8\u8bfb\u5199\u3002sambamba\u652f\u6301\u4e86samtools\u548cpicard\u7684\u5927\u90e8\u5206\u529f\u80fd\uff0c\u800c\u4e14\u901f\u5ea6\u66f4\u5feb\uff0c\u5185\u5b58\u5360\u7528\u66f4\u5c11\uff0c\u64cd\u4f5c\u66f4\u7b80\u5355\u3002sambamba\u4e0d\u4ec5\u53ef\u4ee5\u5bf9BAM\u6587\u4ef6\u8fdb\u884c\u6392\u5e8f\u3001\u7d22\u5f15\u3001\u8fc7\u6ee4\u3001\u7edf\u8ba1\u3001\u6807\u8bb0\u91cd\u590d\u7b49\u5e38\u89c1\u7684\u64cd\u4f5c\uff0c\u8fd8\u53ef\u4ee5\u8fdb\u884c\u4e00\u4e9b\u7279\u6b8a\u7684\u529f\u80fd\uff0c\u6bd4\u5982\u533a\u57df\u8fc7\u6ee4\uff0c\u6807\u8bb0\u91cd\u590d\u5e8f\u5217\uff0c\u68c0\u6d4b\u7ed3\u6784\u53d8\u5f02\u7b49\u3002sambamba\u8fd8\u652f\u6301\u591a\u79cd\u8f93\u5165\u548c\u8f93\u51fa\u683c\u5f0f\uff0c\u6bd4\u5982CRAM\u3001SAM\u3001BED\u3001VCF\u7b49\uff0c\u8ba9\u4f60\u53ef\u4ee5\u7075\u6d3b\u5730\u5904\u7406\u5404\u79cd\u6570\u636e\u7c7b\u578b\u3002<\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;margin-top: 15.6pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">\u4e0b\u8f7d\u7f51\u5740\uff1a<\/span><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">https:\/\/github.com\/biod\/sambamba\/releases<\/span><span style=\"font-size: 15px;\">    <\/span><o:page><\/o:page><\/p>\n<p style=\"text-align: left;margin-left: 0pt;line-height: normal;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.525\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_eJRzxkWY1pdIibPMHcpUDDhicoOhkw.png?resize=553%2C290\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAz3JeicJYr7pOY6FNYBTEIa6t1Af5PicTic90eWmzdHvcfeQicMWheJRzxkWY1pdIibPMHcpUDDhicoOhkw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"290 .4700012207031\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">\u53ef\u4ee5\u901a\u8fc7\u4ee5\u4e0b\u65b9\u6cd5\u4e0b\u8f7d\u5e76\u5b89\u88c5\uff1a<\/span><span style=\"font-family: \u7b49\u7ebf;font-size: 15px;text-indent: 0em;letter-spacing: 0.034em;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code><span class=\"code-snippet_outer\">git <span class=\"code-snippet__built_in\">clone<\/span> --recursive https:\/\/github.com\/lomereiter\/sambamba.git <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">cd<\/span> sambamba && make sambamba-ldmd2-64<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">\u8f93\u5165\u4ee5\u4e0b\u547d\u4ee4\uff0c\u82e5\u51fa\u73b0\u7248\u672c\u4fe1\u606f\uff0c\u5219\u5b89\u88c5\u6210\u529f\uff01<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">sambamba<\/span> \u2013version<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;color: rgb(5, 99, 193);text-decoration: underline;font-size: 15px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.08299180327868852\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_GSibibg2CGD8HicIt7KAaWsNk6Xibg.png?resize=553%2C45\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAz3JeicJYr7pOY6FNYBTEIa6ibWl8Iroov3Ricz9wX4jjMGFcPCPNzGSibibg2CGD8HicIt7KAaWsNk6Xibg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"976\" height=\"45 .92999267578125\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u770b\u4e00\u4e0bsambambad \u529f\u80fd\u5427\uff01<\/span><span style=\"font-family: -apple-system;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">Sambamba\u7684\u4e3b\u8981\u529f\u80fd\u5982\u4e0b\uff1a<\/span><\/span><\/p>\n<p style=\"margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: -apple-system;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">sort: \u5bf9SAM\/BAM\u6587\u4ef6\u8fdb\u884c\u6392\u5e8f\uff0c\u53ef\u4ee5\u6309\u7167\u4f4d\u7f6e\u6216\u8005\u540d\u79f0\u6392\u5e8f\uff0c\u652f\u6301\u591a\u7ebf\u7a0b\u548c\u538b\u7f29\u3002<\/span><\/p>\n<p style=\"margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: -apple-system;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">index: \u5bf9BAM\u6587\u4ef6\u5efa\u7acb\u7d22\u5f15\uff0c\u53ef\u4ee5\u52a0\u901f\u540e\u7eed\u7684\u67e5\u770b\u548c\u5207\u7247\u64cd\u4f5c\u3002<\/span><\/p>\n<p style=\"margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: -apple-system;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">view: \u67e5\u770bSAM\/BAM\u6587\u4ef6\u7684\u5185\u5bb9\uff0c\u53ef\u4ee5\u6307\u5b9a\u8f93\u51fa\u683c\u5f0f\u548c\u8fc7\u6ee4\u6761\u4ef6\uff0c\u4e5f\u53ef\u4ee5\u67e5\u770b\u53c2\u8003\u5e8f\u5217\u7684\u4fe1\u606f\u3002<\/span><\/p>\n<p style=\"margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: -apple-system;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">merge: \u5408\u5e76\u591a\u4e2aBAM\u6587\u4ef6\uff0c\u53ef\u4ee5\u81ea\u52a8\u5904\u7406header\u548c\u538b\u7f29\u7ea7\u522b\u3002<\/span><\/p>\n<p style=\"margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: -apple-system;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">flagstat: \u7edf\u8ba1BAM\u6587\u4ef6\u4e2d\u7684reads\u7684\u6807\u5fd7\u4f4d\uff0c\u53ef\u4ee5\u663e\u793aQC\u901a\u8fc7\u548c\u5931\u8d25\u7684reads\u6570\u76ee\uff0c\u4ee5\u53ca\u5404\u79cd\u914d\u5bf9\u60c5\u51b5\u548c\u91cd\u590d\u60c5\u51b5\u3002<\/span><\/p>\n<p style=\"margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"font-family: -apple-system;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">markdup: \u6807\u8bb0\u6216\u8005\u79fb\u9664BAM\u6587\u4ef6\u4e2d\u7684\u91cd\u590dreads\uff0c\u53ef\u4ee5\u8bbe\u7f6e\u4e34\u65f6\u6587\u4ef6\u76ee\u5f55\u548c\u538b\u7f29\u7ea7\u522b\u3002<\/span>    <\/span><o:page><\/o:page><\/p>\n<p style=\"margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: -apple-system;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">slice: \u63d0\u53d6BAM\u6587\u4ef6\u4e2d\u7684\u67d0\u4e2a\u533a\u57df\uff0c\u53ef\u4ee5\u6307\u5b9a\u8f93\u51fa\u683c\u5f0f\u548c\u8fc7\u6ee4\u6761\u4ef6\u3002<\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">subsample\uff1a<\/span><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u5bf9BAM\u6587\u4ef6\u8fdb\u884c\u5b50\u91c7\u6837\u3002\u5b50\u91c7\u6837\u662f\u6307\u4ece\u539f\u59cb\u6570\u636e\u4e2d\u968f\u673a\u9009\u62e9\u4e00\u90e8\u5206\u6570\u636e\uff0c\u4ee5\u4fbf\u5728\u4fdd\u7559\u539f\u59cb\u6570\u636e\u7279\u5f81\u7684\u540c\u65f6\u51cf\u5c11\u6570\u636e\u91cf\uff0c\u53ef\u4ee5\u63d0\u9ad8\u8ba1\u7b97\u6548\u7387\u3002<\/span><\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">depth       \u8f93\u51fa\u7edf\u8ba1\u4fe1\u606f\uff08BAM\uff09<\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">validate    \u7b80\u5355\u9a8c\u8bc1\u5668\uff08BAM\uff09<\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">\u4e0d\u518d\u652f\u6301\u53d8\u5f02\u68c0\u6d4b\uff08\u53ef\u4ee5\u7528bcftools\uff09<\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;line-height: normal;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.6268806419257773\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_sq2Kur3RA3giczg2noOnJkAKvNd9qA.png?resize=553%2C347\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAz3JeicJYr7pOY6FNYBTEIa6r5QhllHoqdZCqHwrKpKkZyFmBsq2Kur3RA3giczg2noOnJkAKvNd9qA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"997\" height=\"347 .07000732421875\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;line-height: normal;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-size: 15px;text-indent: 0em;letter-spacing: 0.034em;\">\u5bf9\u4e8e\u4e0a\u9762\u6784\u5efa\u7d22\u5f15\u65f6\u51fa\u73b0\u7684\u7684\u9519\u8bef\uff0c\u7528sambamba\u518d\u5c1d\u8bd5\u4ee5\u4e0b\uff1a<\/span><span style=\"font-family: \u7b49\u7ebf;font-size: 15px;text-indent: 0em;letter-spacing: 0.034em;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">sambamba<\/span> <span class=\"code-snippet__selector-tag\">index<\/span> <span class=\"code-snippet__selector-tag\">test<\/span><span class=\"code-snippet__selector-class\">.sort<\/span><span class=\"code-snippet__selector-class\">.bam<\/span> <span class=\"code-snippet__selector-tag\">test<\/span><span class=\"code-snippet__selector-class\">.sort<\/span><span class=\"code-snippet__selector-class\">.bam<\/span><span class=\"code-snippet__selector-class\">.bai<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: left;margin-left: 0pt;line-height: normal;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-indent: 0em;\"><span style=\"font-size: 15px;text-indent: 0em;letter-spacing: 0.034em;font-family: \u7b49\u7ebf;font-variant-numeric: normal;font-variant-east-asian: normal;\">nice\uff01\u6210\u529f\u5566\uff01\u800c\u4e14\u6211\u7684\u6587\u4ef6\u662f41GB\uff0c\u4ec5\u7528\u4e86\u4e24\u5206\u949f\u5c31\u5b8c\u6210\u4e86\uff01<\/span><span style=\"font-size: 15px;text-indent: 0em;letter-spacing: 0.034em;\">    <\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;line-height: normal;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.88125\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_DkedmBVWk64ftZYmlTPloibxRW9mdA.gif?resize=131%2C115\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_gif\/zcicibWZessAz3JeicJYr7pOY6FNYBTEIa64wDeZRIQAXAAlcTlWibfJt4djuDkedmBVWk64ftZYmlTPloibxRW9mdA\/640?wx_fmt=gif'\" data-type=\"gif\" data-w=\"320\" height=\"115 .67999267578125\" width=\"131 .1999969482422\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;margin-top: 15.6pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">\u8fd9\u53ea\u662f\u4e00\u4e2a\u6784\u5efa\u7d22\u5f15\u7684\u4f8b\u5b50\uff0csambamba\u8fd8\u53ef\u4ee5\u505a\u5f88\u591a\u5176\u4ed6\u6709\u7528\u7684\u4e8b\u60c5\u3002\u6216\u8005\uff0c\u4f60\u53ef\u4ee5\u7528\u5b83\u6765\u8f6c\u6362\u6587\u4ef6\u683c\u5f0f\uff0c\u7edf\u8ba1BAM\u6587\u4ef6\u4e2d\u7684\u8986\u76d6\u5ea6\u3001\u6df1\u5ea6\u3001\u6620\u5c04\u8d28\u91cf\u7b49\u4fe1\u606f\u3002<\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;margin-top: 15.6pt;line-height: 150%;text-indent: 0em;\"><span style=\"line-height: 150%;font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-weight: bold;font-size: 15px;\">\u60c5\u666f\u4e00\uff1aSAM\u8f6cBAM<\/span><\/p>\n<p style=\"margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"font-family: -apple-system;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">Sambamba \u7684<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">view<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u547d\u4ee4\u53ef\u4ee5\u7528\u6765\u67e5\u770bSAM\/BAM\u683c\u5f0f\u6587\u4ef6\u7684\u5185\u5bb9\u5e76\u8fdb\u884c\u683c\u5f0f\u8f6c\u6362\u3002\u5b83\u63d0\u4f9b\u4e86\u8bb8\u591a\u9009\u9879\u6765\u63a7\u5236\u8f93\u51fa\uff0c\u5305\u62ec\uff1a<\/span><\/span><\/p>\n<p style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"line-height: 150%;font-family: Symbol;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u00b7<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">-F, –filter=FILTER<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\uff1a\u8bbe\u7f6e\u5bf9\u9f50\u7684\u81ea\u5b9a\u4e49\u8fc7\u6ee4\u5668\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"line-height: 150%;font-family: Symbol;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u00b7<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">–num-filter=NUMFILTER<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\uff1a\u8fc7\u6ee4\u6807\u5fd7\u4f4d\uff1b’i1\/i2\u2019\u5bf9\u5e94\u4e8esamtools\u7684-f i1 -F i2\u53c2\u6570\uff1b\u4e24\u4e2a\u6570\u5b57\u90fd\u53ef\u4ee5\u7701\u7565\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"line-height: 150%;font-family: Symbol;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u00b7<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">-f, –format=sam|bam|json|unpack<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\uff1a\u6307\u5b9a\u8f93\u51fa\u683c\u5f0f\uff08\u9ed8\u8ba4\u4e3aSAM\uff09\uff1bunpack\u6d41\u89e3\u538bBAM\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"line-height: 150%;font-family: Symbol;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u00b7<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">-h, –with-header<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\uff1a\u5728\u8bfb\u53d6\u524d\u6253\u5370\u6807\u9898\uff08BAM\u8f93\u51fa\u59cb\u7ec8\u5982\u6b64\uff09\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"line-height: 150%;font-family: Symbol;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u00b7<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">-H, –header<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\uff1a\u4ec5\u5c06\u6807\u9898\u8f93\u51fa\u5230stdout\uff08\u5982\u679cformat=bam\uff0c\u5219\u6807\u9898\u4ee5SAM\u683c\u5f0f\u6253\u5370\uff09\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"line-height: 150%;font-family: Symbol;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u00b7<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">-I, –reference-info<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\uff1a\u4ec5\u5c06\u53c2\u8003\u540d\u79f0\u548c\u957f\u5ea6\u4ee5JSON\u683c\u5f0f\u8f93\u51fa\u5230stdout\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"line-height: 150%;font-family: Symbol;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u00b7<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">-L, –regions=FILENAME<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\uff1a\u4ec5\u8f93\u51fa\u4e0eBED\u6587\u4ef6\u4e2d\u7684\u4e00\u4e2a\u533a\u57df\u91cd\u53e0\u7684\u8bfb\u53d6\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"line-height: 150%;font-family: Symbol;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u00b7<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">-c, –count<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\uff1a\u4ec5\u5c06\u5339\u914d\u8bb0\u5f55\u6570\u8f93\u51fa\u5230stdout\uff0chHI\u88ab\u5ffd\u7565\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"line-height: 150%;font-family: Symbol;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u00b7<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">-v, –valid<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\uff1a\u4ec5\u8f93\u51fa\u6709\u6548\u5bf9\u9f50\u3002<\/span>    <\/span><o:page><\/o:page><\/p>\n<p style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"line-height: 150%;font-family: Symbol;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u00b7<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">-S, –sam-input<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\uff1a\u6307\u5b9a\u8f93\u5165\u4e3aSAM\u683c\u5f0f\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"line-height: 150%;font-family: Symbol;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u00b7<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">-T, –ref-filename=FASTA<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\uff1a\u6307\u5b9a\u5199\u5165\u53c2\u8003\uff08NA\uff09\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"line-height: 150%;font-family: Symbol;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u00b7<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">-p, –show-progress<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\uff1a\u5728STDERR\u4e2d\u663e\u793a\u8fdb\u5ea6\u6761\uff08\u4ec5\u9002\u7528\u4e8e\u672a\u6307\u5b9a\u533a\u57df\u7684BAM\u6587\u4ef6\uff09\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"line-height: 150%;font-family: Symbol;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u00b7<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">-l, –compression-level<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\uff1a\u6307\u5b9a\u538b\u7f29\u7ea7\u522b\uff08\u4ece0\u52309\uff0c\u4ec5\u9002\u7528\u4e8eBAM\u8f93\u51fa\uff09\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"line-height: 150%;font-family: Symbol;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u00b7<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">-o, –output-filename<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\uff1a\u6307\u5b9a\u8f93\u51fa\u6587\u4ef6\u540d\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"line-height: 150%;font-family: Symbol;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u00b7<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">-t, –nthreads=NTHREADS<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\uff1a\u4f7f\u7528\u7684\u6700\u5927\u7ebf\u7a0b\u6570\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"line-height: 150%;font-family: Symbol;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u00b7<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">-s, –subsample=FRACTION<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\uff1a\u5b50\u91c7\u6837\u8bfb\u53d6\uff08\u8bfb\u53d6\u5bf9\uff09\u3002<\/span><\/span><\/p>\n<p style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: left;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"line-height: 150%;font-family: Symbol;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u00b7<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">–subsampling-seed=SEED<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\uff1a\u8bbe\u7f6e\u5b50\u91c7\u6837\u79cd\u5b50\u3002<\/span><\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;margin-top: 15.6pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">\u4f8b\uff1asambamba view -S -f bam -p input.sam -o output.bam<\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;margin-top: 15.6pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">\u5728\u4e0a\u9762\u7684\u547d\u4ee4\u4e2d\uff0c<\/span><code style=\"mso-ansi-font-size: 12.0pt;mso-bidi-font-family: \u5b8b\u4f53;font-size: 10.5pt;mso-bidi-font-size: 10.5pt;font-family: \u5b8b\u4f53;mso-ascii-font-family: \u5b8b\u4f53;mso-fareast-font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: #111111;\"><span style=\"font-size: 15px;\">-S<\/span><\/code><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">\u9009\u9879\u6307\u5b9a\u8f93\u5165\u6587\u4ef6\u4e3aSAM\u683c\u5f0f\uff0c<\/span><code style=\"mso-ansi-font-size: 12.0pt;mso-bidi-font-family: \u5b8b\u4f53;font-size: 10.5pt;mso-bidi-font-size: 10.5pt;font-family: \u5b8b\u4f53;mso-ascii-font-family: \u5b8b\u4f53;mso-fareast-font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: #111111;\"><span style=\"font-size: 15px;\">-f bam<\/span><\/code><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">\u9009\u9879\u6307\u5b9a\u8f93\u51fa\u683c\u5f0f\u4e3aBAM\uff0c<\/span><code style=\"mso-ansi-font-size: 12.0pt;mso-bidi-font-family: \u5b8b\u4f53;font-size: 10.5pt;mso-bidi-font-size: 10.5pt;font-family: \u5b8b\u4f53;mso-ascii-font-family: \u5b8b\u4f53;mso-fareast-font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: #111111;\"><span style=\"font-size: 15px;\">-p<\/span><\/code><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">\u9009\u9879\u6307\u5b9a\u663e\u793a\u8fdb\u5ea6\u6761\uff0c<\/span><code style=\"mso-ansi-font-size: 12.0pt;mso-bidi-font-family: \u5b8b\u4f53;font-size: 10.5pt;mso-bidi-font-size: 10.5pt;font-family: \u5b8b\u4f53;mso-ascii-font-family: \u5b8b\u4f53;mso-fareast-font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: #111111;\"><span style=\"font-size: 15px;\">input.sam<\/span><\/code><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 15px;\">\u662f\u8f93\u5165\u6587\u4ef6\u7684\u540d\u79f0\uff0c\u800c<\/span><code style=\"mso-ansi-font-size: 12.0pt;mso-bidi-font-family: \u5b8b\u4f53;font-size: 10.5pt;mso-bidi-font-size: 10.5pt;font-family: \u5b8b\u4f53;mso-ascii-font-family: \u5b8b\u4f53;mso-fareast-font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: #111111;\"><span style=\"font-size: 15px;\">-o output.bam<\/span><\/code><span style=\"font-size: 15px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u6307\u5b9a\u8f93\u51fa\u6587\u4ef6\u7684\u540d\u79f0\u3002<\/span><\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;margin-top: 15.6pt;line-height: 150%;text-indent: 0em;\"><span style=\"line-height: 150%;font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-weight: bold;font-size: 15px;\">\u60c5\u666f\u4e8c\uff1a\u7edf\u8ba1\u6bcf\u4e2a\u67d3\u8272\u4f53\u4e0a\u7684\u8986\u76d6\u5ea6<\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;margin-top: 15.6pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">\u4f60\u53ef\u4ee5\u4f7f\u7528\u4ee5\u4e0b\u547d\u4ee4\uff1a<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">sambamba<\/span> <span class=\"code-snippet__selector-tag\">depth<\/span> <span class=\"code-snippet__selector-tag\">base<\/span> <span class=\"code-snippet__selector-tag\">test<\/span><span class=\"code-snippet__selector-class\">.bam<\/span> <span class=\"code-snippet__selector-tag\">-o<\/span> <span class=\"code-snippet__selector-tag\">coverage<\/span><span class=\"code-snippet__selector-class\">.txt<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;margin-top: 15.6pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u8fd9\u6837\u5c31\u4f1a\u751f\u6210\u4e00\u4e2a\u6587\u672c\u6587\u4ef6\uff0c\u5217\u51fa\u4e86\u6bcf\u4e2a\u67d3\u8272\u4f53\u4e0a\u6bcf\u4e2a\u78b1\u57fa\u4f4d\u7f6e\u7684\u8986\u76d6\u5ea6\u3002\u4f60\u53ef\u4ee5\u7528\u7c7b\u4f3c\u7684\u65b9\u5f0f\u7edf\u8ba1\u5176\u4ed6\u4fe1\u606f\uff0c\u6bd4\u5982\u533a\u57df\u8986\u76d6\u5ea6\u3001\u57fa\u56e0\u8986\u76d6\u5ea6\u3001\u6620\u5c04\u8d28\u91cf\u5206\u5e03\u7b49\u3002<\/span>    <\/span><o:page><\/o:page><\/p>\n<p style=\"text-align: left;margin-left: 0pt;line-height: normal;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.6609485368314834\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_bUEaZibzIUiafrmzO1lnUZ4nwFpydA.png?resize=553%2C365\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAz3JeicJYr7pOY6FNYBTEIa6N5ficKvP276IqiaIXTh4zYgic0J8bbUEaZibzIUiafrmzO1lnUZ4nwFpydA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"991\" height=\"365 .94000244140625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;margin-top: 15.6pt;text-align: left;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u5982\u679c\u4f60\u4ec5\u67d0\u6bb5\u57fa\u56e0\u7ec4\u611f\u5174\u8da3\uff0c\u60f3\u8981\u63d0\u53d6\u6bd4\u5bf9\u5230\u8fd9\u6bb5\u533a\u57df\u7684bam\u4fe1\u606f\uff0c\u90a3\u5c31\u7528<\/span><span style=\"font-family: -apple-system;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">slice\u8bd5\u8bd5\u5427\uff0c\u547d\u4ee4\u5982\u4e0b\uff1a<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">slice<\/span> <span class=\"code-snippet__selector-tag\">input<\/span><span class=\"code-snippet__selector-class\">.bam<\/span> <span class=\"code-snippet__selector-tag\">chr1H<\/span><span class=\"code-snippet__selector-pseudo\">:10000-20000<\/span> > <span class=\"code-snippet__selector-tag\">output<\/span><span class=\"code-snippet__selector-class\">.bam<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: left;margin-left: 0pt;line-height: normal;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-size: 15px;text-indent: 0em;letter-spacing: 0.034em;\">\u8fd9\u6837\u6211\u4eec\u5c31\u5f97\u5230\u6211\u4eec\u7684\u9776\u533a\u6587\u4ef6\u5566\uff01<\/span><br \/><\/p>\n<p style=\"text-align: left;margin-left: 0pt;line-height: normal;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.3212962962962963\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_2LIq5oImNePt2TyHEJXv9EbvBJ5wLg.png?resize=553%2C177\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAz3JeicJYr7pOY6FNYBTEIa6iauqjNA7qbib0cyImnQYNicWXN92LIq5oImNePt2TyHEJXv9EbvBJ5wLg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"177 .79998779296875\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;margin-top: 15.6pt;margin-bottom: 15.6pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u751a\u81f3\u53ef\u4ee5\u7528\u5b83\u6765\u6807\u8bb0\u91cd\u590d\u5e8f\u5217\uff0c\u5e76\u4e14\u9009\u62e9\u4fdd\u7559\u6216\u5220\u9664\u8fd9\u4e9b\u91cd\u590d\u5e8f\u5217\u3002\u5047\u8bbe\u4f60\u60f3\u8981\u6807\u8bb0test.bam\u6587\u4ef6\u4e2d\u7684\u91cd\u590d\u5e8f\u5217\uff0c\u5e76\u4e14\u5220\u9664\u5b83\u4eec\uff0c\u4f60\u53ef\u4ee5\u4f7f\u7528\u4ee5\u4e0b\u547d\u4ee4\uff1a<\/span>    <\/span><o:page><\/o:page><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">sambamba<\/span> <span class=\"code-snippet__selector-tag\">markdup<\/span> <span class=\"code-snippet__selector-tag\">-r<\/span> <span class=\"code-snippet__selector-tag\">test<\/span><span class=\"code-snippet__selector-class\">.bam<\/span> <span class=\"code-snippet__selector-tag\">dedup<\/span><span class=\"code-snippet__selector-class\">.bam<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;margin-top: 15.6pt;margin-bottom: 15.6pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">-r \u8868\u793a\u5220\u9664\u91cd\u590d\uff0c\u9ed8\u8ba4\u4ec5\u6807\u8bb0\u4e0d\u5220\u9664\u3002\u8fd9\u6837\u5c31\u4f1a\u751f\u6210\u4e00\u4e2a\u65b0\u7684BAM\u6587\u4ef6\uff0c\u53bb\u9664\u4e86\u6240\u6709\u88ab\u6807\u8bb0\u4e3a\u91cd\u590d\u7684reads\u3002\u4f60\u4e5f\u53ef\u4ee5\u9009\u62e9\u4fdd\u7559\u91cd\u590d\u5e8f\u5217\uff0c\u4f46\u662f\u7ed9\u5b83\u4eec\u6dfb\u52a0\u4e00\u4e2a\u6807\u7b7e\uff0c\u4ee5\u4fbf\u4e8e\u540e\u7eed\u5206\u6790\u3002<\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;margin-top: 15.6pt;margin-bottom: 15.6pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;\"><span style=\"font-size: 15px;font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u603b\u4e4b\uff0csambamba\u662f\u4e00\u4e2a\u529f\u80fd\u5f3a\u5927\u3001\u6027\u80fd\u4f18\u5f02\u3001\u6613\u4e8e\u4f7f\u7528\u7684BAM\u6587\u4ef6\u5904\u7406\u5de5\u5177\uff0c\u5b83\u53ef\u4ee5\u8ba9\u4f60\u7684\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u66f4\u52a0\u9ad8\u6548\u548c\u6109\u5feb\u3002\u5982\u679c\u4f60\u8fd8\u6ca1\u6709\u5c1d\u8bd5\u8fc7sambamba\uff0c\u90a3\u4e48\u8d76\u5feb\u53bb\u4e0b\u8f7d\u5427\uff01<\/span><\/span><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;background-color: rgb(255, 255, 255);text-align: left;\">\n<section style=\"outline: 0px;font-size: 16px;\">\n<section powered-by=\"xiumi.us\" style=\"margin: 10px;outline: 0px;justify-content: flex-start;display: flex;flex-flow: row;\">\n<section style=\"padding-right: 10px;padding-left: 10px;outline: 0px;display: inline-block;width: 558px;vertical-align: top;border-style: dashed;border-width: 1px;border-color: rgb(62, 62, 62);align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;outline: 0px;\">\n<section style=\"outline: 0px;display: inline-block;width: 536px;border-width: 0px 0px 0px 10px;border-style: solid;border-left-color: rgb(144, 215, 236);border-right-color: rgb(46, 172, 109);box-shadow: rgb(0, 0, 0) 0px 0px 0px;border-bottom-left-radius: 0px;\">\n<section powered-by=\"xiumi.us\" style=\"padding-right: 3px;padding-left: 3px;outline: 0px;\">\n<section style=\"margin-top: 0.95em;margin-bottom: -0.65em;outline: 0px;display: inline-block;width: 520px;vertical-align: top;\">\n<section style=\"margin-top: -1em;padding: 3px 10px;outline: 0px;display: inline-block;vertical-align: top;color: rgb(0, 154, 68);\">\n<p style=\"outline: 0px;\"><strong style=\"outline: 0px;\">\u5f80\u671f\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: justify;\">\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491794&idx=1&sn=2b7a4de95169c79202e44813885a8250&chksm=cf7f7017f808f9015689839fe4057fd418bf7b20eb1dfb5a28cd92e56a41b5605e62bfbc16fb&scene=21#wechat_redirect\" textvalue=\"1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;font-size: 14px;\" rel=\"noopener\">1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4<\/a><br style=\"outline: 0px;\" \/><\/section>\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491705&idx=1&sn=9ff9b6da28b3a256a99c6697d589be66&chksm=cf7f70bcf808f9aafd1019a0bba3dd3a55e2867dbb9a09203f13c2683adf867094637c405ed1&scene=21#wechat_redirect\" textvalue=\"2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;font-size: 14px;\" 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