{"id":55681,"date":"2024-06-16T17:07:40","date_gmt":"2024-06-16T09:07:40","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55681"},"modified":"2024-06-16T17:07:40","modified_gmt":"2024-06-16T09:07:40","slug":"%e8%a7%a3%e9%94%81%e6%95%b0%e6%8d%ae%e5%a5%a5%e7%a7%98%ef%bc%9a%e7%94%a8pheatmap%e6%89%93%e9%80%a0%e7%b2%be%e7%be%8e%e7%83%ad%e5%9b%be","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55681","title":{"rendered":"\u89e3\u9501\u6570\u636e\u5965\u79d8\uff1a\u7528pheatmap\u6253\u9020\u7cbe\u7f8e\u70ed\u56fe"},"content":{"rendered":"


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none;\">\u8fd9\u91cc\u7684\u6570\u636e\u96c6\u5c0f\u679c\u5df2\u7ecf\u4e3a\u5927\u5bb6\u51c6\u5907\u597d\u5566\uff0c\u5927\u5bb6\u53ef\u4ee5\u76f4\u63a5\u7ed8\u56fe\u6216\u8005\u53c2\u8003~<\/span>    <\/span><o:page><\/o:page><\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;\">\u4f46\u662f\u8981\u6ce8\u610f\u6570\u636e\u96c6\u8981\u6709\u4ee5\u4e0b\u6761\u4ef6\u54e6~<\/span><\/section>\n<section style=\"text-align: justify;font-size: 10.5pt;font-family: Calibri;font-weight: normal;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;\">1.<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u5b8c\u6574\u2014\u2014\u6570\u636e\u96c6\u5e94\u5305\u542b\u6240\u6709\u5fc5\u8981\u7684\u4fe1\u606f\uff0c\u4ee5\u652f\u6301\u56fe\u5f62\u7684\u7ed8\u5236\u3002<\/span><\/span><\/section>\n<section style=\"text-align: justify;font-size: 10.5pt;font-family: Calibri;font-weight: normal;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;\">2.<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u51c6\u786e\u2014\u2014\u9891\u7387\u4f30\u8ba1\u5e94\u57fa\u4e8e\u53ef\u9760\u7684\u6570\u636e\u548c\u5408\u7406\u7684\u5047\u8bbe\u3002\u4e0d\u51c6\u786e\u7684\u6570\u636e\u53ef\u80fd\u5bfc\u81f4\u56fe\u5f62\u7684\u8bef\u5224\u3002<\/span><\/span><\/section>\n<section style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: justify;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;\">3.<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u76f8\u5173\u6027\u2014\u2014\u6570\u636e\u96c6\u4e2d\u7684\u6837\u672c\u987a\u5e8f\u5e94\u8be5\u6309\u7167\u98ce\u9669\u5206\u6570\u4ece\u4f4e\u5230\u9ad8\u6392\u5e8f\uff0c\u4ee5\u786e\u4fdd\u98ce\u9669\u66f2\u7ebf\u80fd\u591f\u6b63\u786e\u53cd\u6620\u6837\u672c\u7684\u98ce\u9669\u6c34\u5e73\u53d8\u5316\u3002<\/span><\/span><\/section>\n<section style=\"font-size: 10.5pt;font-family: Calibri;font-weight: normal;text-align: justify;margin-left: 22pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018106\" data-ratio=\"0.9402985074626866\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-04-29_\u89e3\u9501\u6570\u636e\u5965\u79d8\uff1a\u7528pheatmap\u6253\u9020\u7cbe\u7f8e\u70ed\u56fe\/2_hX71lJUosolBFNia8Bmtg511V6wR9Q.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAw1Y3OHHbdbv0YISSRgfb3QRt4rRvWxQVlwkwChldooIcUu2hX71lJUosolBFNia8Bmtg511V6wR9Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1072\" height=\"426.6099853515625\" width=\"408.010009765625\" \/><\/span><\/section>\n<h2 style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: Cambria;font-variant: normal;text-transform: none;\">\u4e8c\u3001Pheatmap R\u5305\u88c5\u8f7d<\/span><br \/>\n  <\/span><\/h2>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);font-size: 16px;\"><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"sql\"><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"pheatmap\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"limma \"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">library<\/span>(pheatmap)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">library<\/span>(<span class=\"code-snippet__string\">\"limma\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);font-size: 16px;\"><\/span><\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(255, 0, 0);font-weight: bold;\">\u5c0f\u4f19\u4f34\u4eec\u8981\u662f\u89c9\u5f97\u81ea\u5df1\u4e0b\u8f7dR\u5305\u64cd\u4f5c\u592a\u9ebb\u70e6\uff0c\u53ef\u4ee5\u4f7f\u7528\u76f8\u5173\u670d\u52a1\u5668\u54e6~\u6b22\u8fce\u8054\u7cfb\u5c0f\u679c\u79df\u8d41\u6027\u4ef7\u6bd4\u5c45\u9ad8\u7684\u670d\u52a1\u5668\uff01\u5c0f\u679c\u968f\u65f6\u606d\u5019\uff01<\/span><\/span><\/section>\n<section style=\"font-size: 15px;padding-right: 2px;padding-left: 2px;margin-bottom: 0px;\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;\">\n<section style=\"display: flex;width: 100%;flex-flow: column;\" powered-by=\"xiumi.us\">\n<section style=\"z-index: 1;transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;transform: translate3d(4px, 0px, 0px) rotateX(180deg);margin-bottom: -2px;\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 10px;height: 14px;vertical-align: top;overflow: hidden;background-color: rgb(91, 91, 91);border-style: solid;border-width: 2px;border-color: rgb(255, 255, 255);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 10px;height: 14px;vertical-align: top;overflow: hidden;background-color: rgb(91, 91, 91);border-style: solid;border-width: 2px;border-color: rgb(255, 255, 255);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(194, 209, 255);\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row;margin-top: -4px;margin-bottom: 5px;transform: translate3d(5px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(193, 209, 255);padding: 10px 20px;box-shadow: rgb(255, 255, 255) -2px 2px 0px;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;align-self: center;flex: 0 0 auto;width: 100%;height: auto;\">\n<section style=\"text-align: center;color: rgb(0, 0, 0);\" powered-by=\"xiumi.us\">\n<p><strong>\u516c\u4f17\u53f7\u540e\u53f0\u56de\u590d\u201c111\u201d<\/strong><\/p>\n<p><strong>\u9886\u53d6\u672c\u7bc7\u4ee3\u7801\u3001\u57fa\u56e0\u96c6\u6216\u793a\u4f8b\u6570\u636e\u7b49\u6587\u4ef6<\/strong><\/p>\n<p><strong>\u6587\u4ef6\u7f16\u53f7\uff1a240423<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><span style=\"color: rgb(171, 25, 66);\"><strong>\u9700\u8981\u79df\u8d41\u670d\u52a1\u5668\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u626b\u7801\u6dfb\u52a0\u5c0f\u679c\uff0c\u6b64\u5916\u5c0f\u679c\u8fd8\u63d0\u4f9b\u751f\u4fe1\u5206\u6790\uff0c\u601d\u8def\u8bbe\u8ba1\uff0c\u6587\u732e\u590d\u73b0\u7b49\uff0c\u6709\u9700\u8981\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u6765\u64a9~<\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100018109\" data-ratio=\"1\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-04-29_\u89e3\u9501\u6570\u636e\u5965\u79d8\uff1a\u7528pheatmap\u6253\u9020\u7cbe\u7f8e\u70ed\u56fe\/3_f6PG40Qrdu5e9B1iaIMItPiajABYWA.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxqaqPbQprPd4vw8Ytp0cprbzXiakMDdFriaB6B59ibhlBCrNyQFf6PG40Qrdu5e9B1iaIMItPiajABYWA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"430\" style=\"width: 170px;height: 170px;\" \/><span style=\"background-color: rgb(255, 255, 255);font-size: 16px;letter-spacing: 0.034em;text-indent: 0em;text-align: justify;\">   <\/span><\/p>\n<\/section>\n<p style=\"margin-bottom: 0px;font-size: 16px;letter-spacing: 0.578px;text-wrap: wrap;background-color: rgb(255, 255, 255);\"><span style=\"text-decoration: underline;color: rgb(255, 41, 65);\"><\/span><\/p>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><o:page><\/o:page><\/section>\n<h2 style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: Cambria;font-variant: normal;text-transform: none;\">\u4e09\u3001<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u6837\u54c1\u6587\u4ef6\u7684\u5dee\u5f02\u5206\u6790\u5904\u7406<\/span><br \/>\n  <\/span><\/h2>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;\">\u4f9d\u7136\u5148\u5c06\u5de5\u4f5c\u8def\u5f84\u8fdb\u884c\u4fee\u6539\u8bbe\u7f6e<\/span><\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);font-size: 16px;\"><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__section\">setwd(\"F:\\ 96glycolysis\\09.glyGeneExp\\m6a\\8\") <\/span><\/span><\/code><code><span class=\"code-snippet_outer\">inputFile=<span class=\"code-snippet__string\">\"sampleExp.txt\"<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8f93\u5165\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pvalFilter=0.05 <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#p\u503c\u4e34\u754c\u503c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">logFCfilter=0 <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#logFC\u4e34\u754c\u503c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">conNum=41 <span class=\"code-snippet__comment\">#normal\u7ec4\u6837\u54c1\u6570\u76ee<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">treatNum=476 <span class=\"code-snippet__comment\">#tumor\u7ec4\u6837\u54c1\u6570\u76ee<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6\u8f93\u5165\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">outTab=data.frame()<\/span><\/code><code><span class=\"code-snippet_outer\">grade=c(rep(1,conNum),rep(2,treatNum))<\/span><\/code><code><span class=\"code-snippet_outer\">rt=read.table(inputFile,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,header=T,check.names=F)<\/span><\/code><code><span class=\"code-snippet_outer\">rt=as.matrix(rt)<\/span><\/code><code><span class=\"code-snippet_outer\">rownames(rt)=rt[,1]<\/span><\/code><code><span class=\"code-snippet_outer\">exp=rt[,2:ncol(rt)]<\/span><\/code><code><span class=\"code-snippet_outer\">dimnames=list(rownames(exp),colnames(exp))<\/span><\/code><code><span class=\"code-snippet_outer\">data=matrix(as.numeric(as.matrix(exp)),nrow=nrow(exp),dimnames=dimnames) <\/span><\/code><code><span class=\"code-snippet_outer\">data=avereps(data)<\/span><\/code><code><span class=\"code-snippet_outer\">data=data[rowMeans(data)>0,]<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5dee\u5f02\u5206\u6790<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">for(i in row.names(data)){<\/span><\/code><code><span class=\"code-snippet_outer\"> geneName=unlist(strsplit(i,<span class=\"code-snippet__string\">\"\\|\"<\/span>,))[1]<\/span><\/code><code><span class=\"code-snippet_outer\"> geneName=gsub(<span class=\"code-snippet__string\">\"\\\/\"<\/span>, <span class=\"code-snippet__string\">\"_\"<\/span>, geneName)<\/span><\/code><code><span class=\"code-snippet_outer\"> rt=rbind(expression=data[i,],grade=grade)<\/span><\/code><code><span class=\"code-snippet_outer\"> rt=as.matrix(t(rt))<\/span><\/code><code><span class=\"code-snippet_outer\"> wilcoxTest<-wilcox.test(expression ~ grade, data=rt)<\/span><\/code><code><span class=\"code-snippet_outer\"> conGeneMeans=mean(data[i,1:conNum])<\/span><\/code><code><span class=\"code-snippet_outer\"> treatGeneMeans=mean(data[i,(conNum+1):ncol(data)])<\/span><\/code><code><span class=\"code-snippet_outer\"> logFC=log2(treatGeneMeans)-log2(conGeneMeans)<\/span><\/code><code><span class=\"code-snippet_outer\"> pvalue=wilcoxTest$p.value<\/span><\/code><code><span class=\"code-snippet_outer\"> conMed=median(data[i,1:conNum])<\/span><\/code><code><span class=\"code-snippet_outer\"> treatMed=median(data[i,(conNum+1):ncol(data)])<\/span><\/code><code><span class=\"code-snippet_outer\"> diffMed=treatMed-conMed<\/span><\/code><code><span class=\"code-snippet_outer\"> if( ((logFC>0) & (diffMed>0)) | ((logFC<0) & (diffMed<0)) ){ <\/span><\/code><code><span class=\"code-snippet_outer\"> outTab=rbind(outTab,cbind(gene=i,conMean=conGeneMeans,treatMean=treatGeneMeans,logFC=logFC,pValue=pvalue))<\/span><\/code><code><span class=\"code-snippet_outer\"> }<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8f93\u51fa\u6240\u6709\u57fa\u56e0\u7684\u5dee\u5f02\u60c5\u51b5<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">write.table(outTab,file=<span class=\"code-snippet__string\">\"all.txt\"<\/span>,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,row.names=F,quote=F)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8f93\u51fa\u5dee\u5f02\u8868\u683c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">outDiff=outTab[(abs(as.numeric(as.vector(outTab$logFC)))>logFCfilter & as.numeric(as.vector(outTab$pValue)) <pvalFilter),]< span><\/pvalFilter),]<><\/span><\/code><code><span class=\"code-snippet_outer\">write.table(outDiff,file=<span class=\"code-snippet__string\">\"diff.xls\"<\/span>,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,row.names=F,quote=F)<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(outDiff,file=<span class=\"code-snippet__string\">\"diff.txt\"<\/span>,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,row.names=F,quote=F)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8f93\u51fa\u5dee\u5f02\u57fa\u56e0\u7684\u8868\u8fbe\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">heatmap=rbind(ID=colnames(data[as.vector(outDiff[,1]),]),data[as.vector(outDiff[,1]),])<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(heatmap,file=<span class=\"code-snippet__string\">\"diffGeneExp.txt\"<\/span>,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,col.names=F,quote=F)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\">\u56db\u3001\u6587\u4ef6\u7684\u5bfc\u5165<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\">\u4f9d\u7136\u5148\u5c06\u5de5\u4f5c\u8def\u5f84\u8fdb\u884c\u4fee\u6539\u8bbe\u7f6e<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__section\">setwd(\"F:\\ 96glycolysis\\09.glyGeneExp\\m6a\\8\") <\/span><\/span><\/code><code><span class=\"code-snippet_outer\">rt=read.table(<span class=\"code-snippet__string\">\"risk.txt\"<\/span>,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,header=T,row.names=1,check.names=F) <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6\u8f93\u5165\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">rt=rt[order(rt$riskScore),] <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6309\u7167riskScore\u5bf9\u6837\u54c1\u6392\u5e8f<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4f7f\u7528read.table()\u51fd\u6570\u8bfb\u53d6\u540d\u4e3a\"risk.txt\"\u7684\u6570\u636e\u6587\u4ef6\uff0c\u5e76\u5b58\u50a8\u5728\u53d8\u91cfrt\u4e2d\u3002<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(0, 176, 240);font-size: 16px;\"><\/span><\/section>\n<h2 style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: Cambria;font-variant: normal;text-transform: none;\">\u4e94\u3001<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u53c2\u6570\u7684\u8c03\u6574\u4e0e\u56fe\u5f62\u7684\u4fdd\u5b58<\/span><br \/>\n  <\/span><\/h2>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;\">\u5c0f\u679c\u8fd9\u4e00\u671f\u7ed8\u5236\u4e86\u4e09\u7c7b\u56fe\uff1a\u98ce\u9669\u66f2\u7ebf\u3001\u751f\u5b58\u72b6\u6001\u56fe\u3001\u98ce\u9669\u70ed\u56fe\u3002<\/span><\/section>\n<h2 style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Cambria;font-variant: normal;text-transform: none;\">1.<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u98ce\u9669\u66f2\u7ebf<\/span><br \/>\n  <\/span><\/h2>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u98ce\u9669\u66f2\u7ebf\u901a\u5e38\u7528\u4e8e\u63cf\u8ff0\u67d0\u4e2a\u57fa\u56e0\u53d8\u5f02\u3001\u73af\u5883\u56e0\u7d20\u6216\u4e34\u5e8a\u6307\u6807\u4e0e\u75be\u75c5\u98ce\u9669\u4e4b\u95f4\u7684\u5173\u7cfb\u3002<\/span>    <\/span><o:page><\/o:page><\/section>\n<h2 style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Cambria;font-variant: normal;text-transform: none;\">2.<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u751f\u5b58\u72b6\u6001\u56fe<\/span><br \/>\n  <\/span><\/h2>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;\">\u751f\u5b58\u72b6\u6001\u56fe\uff08\u4e5f\u79f0\u4e3a\u751f\u5b58\u66f2\u7ebf\u6216Kaplan-Meier\u66f2\u7ebf\uff09\u5e38\u7528\u4e8e\u63cf\u8ff0\u60a3\u8005\u7fa4\u4f53\u7684\u751f\u5b58\u65f6\u95f4\u5206\u5e03\u3002Kaplan-Meier\u66f2\u7ebf\u5c55\u793a\u4e86\u60a3\u8005\u4ece\u63a5\u53d7\u6cbb\u7597\u5230\u4e8b\u4ef6\u53d1\u751f\uff08\u5982\u6b7b\u4ea1\u6216\u75be\u75c5\u590d\u53d1\uff09\u7684\u65f6\u95f4\u957f\u77ed\u3002<\/span><\/section>\n<h2 style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: Cambria;font-variant: normal;text-transform: none;\">3.<\/span><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u98ce\u9669\u70ed\u56fe<\/span><br \/>\n  <\/span><\/h2>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;\">\u98ce\u9669\u70ed\u56fe\u5e38\u7528\u4e8e\u5c55\u793a\u57fa\u56e0\u7ec4\u6570\u636e\u4e2d\u7684\u98ce\u9669\u6a21\u5f0f\u6216\u5dee\u5f02\u3002\u8fd9\u4e9b\u70ed\u56fe\u53ef\u4ee5\u663e\u793a\u4e0d\u540c\u57fa\u56e0\u3001\u57fa\u56e0\u533a\u57df\u6216\u6837\u672c\u4e4b\u95f4\u7684\u98ce\u9669\u6c34\u5e73\uff0c\u901a\u8fc7\u989c\u8272\u6df1\u6d45\u6216\u8272\u8c03\u53d8\u5316\u6765\u53cd\u6620\u98ce\u9669\u7684\u9ad8\u4f4e\u3002<\/span><\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;\">\u5c0f\u679c\u8fd9\u4e00\u671f\u5c06\u7528pheatmap R\u5305\u8fdb\u884c\u7ed8\u5236\u98ce\u9669\u70ed\u56fe~\u98ce\u9669\u70ed\u56fe\u80fd\u591f\u8ba9\u6211\u4eec\u8bc6\u522b\u4e0e\u75be\u75c5\u76f8\u5173\u7684\u57fa\u56e0\u3001\u57fa\u56e0\u53d8\u5f02\u6216\u751f\u7269\u6807\u8bb0\u7269\uff0c\u7136\u540e\u8fdb\u4e00\u6b65\u63ed\u793a\u75be\u75c5\u7684\u5206\u5b50\u673a\u5236\u548c\u6f5c\u5728\u6cbb\u7597\u9776\u70b9\u3002<\/span><\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(255, 192, 0);font-weight: bold;font-size: 16px;\"><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u98ce\u9669\u66f2\u7ebf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">riskClass=rt[,<span class=\"code-snippet__string\">\"risk\"<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4ece\u6570\u636e\u6846rt\u4e2d\u63d0\u53d6\u540d\u4e3a\"risk\"\u7684\u5217\uff0c\u5c06\u5176\u8d4b\u503c\u7ed9riskClass\u53d8\u91cf\u3002\u8fd9\u4e2a\u5217\u5e94\u8be5\u5305\u542b\u98ce\u9669\u7b49\u7ea7\u7684\u4fe1\u606f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">lowLength=length(riskClass[riskClass==<span class=\"code-snippet__string\">\"low\"<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8ba1\u7b97\u98ce\u9669\u7b49\u7ea7\u4e3a\"low\"\u7684\u6570\u91cf\uff0c\u5c06\u7ed3\u679c\u8d4b\u503c\u7ed9lowLength\u53d8\u91cf\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">highLength=length(riskClass[riskClass==<span class=\"code-snippet__string\">\"high\"<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8ba1\u7b97\u98ce\u9669\u7b49\u7ea7\u4e3a\"high\"\u7684\u6570\u91cf\uff0c\u5c06\u7ed3\u679c\u8d4b\u503c\u7ed9highLength\u53d8\u91cf\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">line=rt[,<span class=\"code-snippet__string\">\"riskScore\"<\/span>] <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4ece\u6570\u636e\u6846rt\u4e2d\u63d0\u53d6\u540d\u4e3a\"riskScore\"\u7684\u5217\uff0c\u5c06\u5176\u8d4b\u503c\u7ed9line\u53d8\u91cf\u3002\u8fd9\u4e2a\u5217\u5e94\u8be5\u5305\u542b\u98ce\u9669\u8bc4\u5206\u7684\u4fe1\u606f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">line[line>10]=10 <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c06line\u4e2d\u5927\u4e8e10\u7684\u503c\u8bbe\u4e3a10\uff0c\u9650\u5236\u98ce\u9669\u8bc4\u5206\u7684\u8303\u56f4\u3002(\u5c0f\u679c\u8bbe\u5b9a\u7684\u503c\u662f10\uff0c\u5c0f\u4f19\u4f34\u4eec\u53ef\u6839\u636e\u81ea\u5df1\u7684\u6570\u636e\u96c6\u8fdb\u884c\u4fee\u6539~)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pdf(file=<span class=\"code-snippet__string\">\"riskScore.pdf\"<\/span>,width = 10,height = 5)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u5bbd\u5ea6\u3001\u9ad8\u5ea6\u3002\uff08\u5c0f\u4f19\u4f34\u4eec\u53ef\u6839\u636e\u81ea\u5df1\u9700\u6c42\u4fee\u6539\u53c2\u6570\uff09<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">plot(line,<\/span><\/code><code><span class=\"code-snippet_outer\"> type=<span class=\"code-snippet__string\">\"p\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> pch=20,<\/span><\/code><code><span class=\"code-snippet_outer\"> xlab=<span class=\"code-snippet__string\">\"Patients (increasing risk socre)\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> ylab=<span class=\"code-snippet__string\">\"Risk score\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> col=c(rep(<span class=\"code-snippet__string\">\"green\"<\/span>,lowLength),<\/span><\/code><code><span class=\"code-snippet_outer\"> rep(<span class=\"code-snippet__string\">\"red\"<\/span>,highLength)))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236\u6563\u70b9\u56fe\uff0c\u6a2a\u5750\u6807\u4e3a\u60a3\u8005\uff0c\u98ce\u9669\u8bc4\u5206\u9012\u589e\uff0c\u7eb5\u5750\u6807\u4e3a\u98ce\u9669\u8bc4\u5206\uff0c\u6839\u636e\u98ce\u9669\u7b49\u7ea7\u4e3a\"low\"\u548c\"high\"\u7684\u6570\u91cf\u6765\u8bbe\u5b9a\u989c\u8272\uff08\u5c0f\u4f19\u4f34\u4eec\u53ef\u6839\u636e\u81ea\u5df1\u7684\u559c\u597d\u4fee\u6539~\uff09<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#plot()\u662fR\u8bed\u8a00\u4e2d\u975e\u5e38\u5e38\u7528\u4e14\u529f\u80fd\u5f3a\u5927\u7684\u7ed8\u56fe\u5185\u7f6e\u51fd\u6570\uff0c\u7528\u4e8e\u7ed8\u5236\u5404\u79cd\u7c7b\u578b\u7684\u56fe\u5f62\uff0c\u5982\u6563\u70b9\u56fe\u3001\u6298\u7ebf\u56fe\u3001\u76f4\u65b9\u56fe\u7b49<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">abline(v=lowLength,lty=5)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5728\u56fe\u4e2d\u6dfb\u52a0\u4e00\u6761\u5782\u76f4\u865a\u7ebf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">legend(<span class=\"code-snippet__string\">\"topleft\"<\/span>,c(<span class=\"code-snippet__string\">\"Highrisk\"<\/span>,<span class=\"code-snippet__string\">\"lowRisk\"<\/span>),bty=<span class=\"code-snippet__string\">\"n\"<\/span>,pch=19,col=c(<span class=\"code-snippet__string\">\"red\"<\/span>,<span class=\"code-snippet__string\">\"green\"<\/span>),cex=1.2)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5728\u5de6\u4e0a\u89d2\u6dfb\u52a0\u56fe\u4f8b\uff0c\u5206\u522b\u8868\u793a\"Highrisk\"\u548c\"lowRisk\"\uff0c\u989c\u8272\u4e3a\u7ea2\u8272\u548c\u7eff\u8272\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">dev.off()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5173\u95edPDF\u6587\u4ef6\u7ed8\u56fe\u8bbe\u5907\uff0c\u4fdd\u5b58\u56fe\u8868<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(0, 176, 240);\"><\/span>    <\/span><o:page><\/o:page><\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018104\" data-ratio=\"0.507\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-04-29_\u89e3\u9501\u6570\u636e\u5965\u79d8\uff1a\u7528pheatmap\u6253\u9020\u7cbe\u7f8e\u70ed\u56fe\/4_l8srFkWNJ6PoplZicKEXsV0iaLuBxA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAw1Y3OHHbdbv0YISSRgfb3Qiaic30krSH1pjRxJUiaCM2B9csEhNl8srFkWNJ6PoplZicKEXsV0iaLuBxA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1000\" height=\"280.739990234375\" width=\"553.739990234375\" \/><\/span><\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(255, 192, 0);font-weight: bold;font-size: 16px;\"><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"objectivec\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u751f\u5b58\u72b6\u6001\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">color=as.vector(rt$fustat)<\/span><\/code><code><span class=\"code-snippet_outer\">color[color==<span class=\"code-snippet__number\">1<\/span>]=<span class=\"code-snippet__string\">\"red\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">color[color==<span class=\"code-snippet__number\">0<\/span>]=<span class=\"code-snippet__string\">\"blue\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u521b\u5efa\u4e00\u4e2a\u5411\u91cfcolor\uff0c\u5176\u4e2d\u7684\u503c\u4e0ert$fustat\u4e2d\u7684\u503c\u76f8\u540c\u3002\u63a5\u7740\u6839\u636ert$fustat\u7684\u503c\uff0c\u5c06color\u5411\u91cf\u4e2d\u4e3a1\u7684\u5143\u7d20\u8bbe\u4e3a<span class=\"code-snippet__meta-string\">\"red\"<\/span>\uff0c\u4e3a0\u7684\u5143\u7d20\u8bbe\u4e3a<span class=\"code-snippet__meta-string\">\"blue\"<\/span>\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pch=as.vector(rt$fustat)<\/span><\/code><code><span class=\"code-snippet_outer\">pch[pch==<span class=\"code-snippet__number\">1<\/span>]=<span class=\"code-snippet__number\">15<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pch[pch==<span class=\"code-snippet__number\">0<\/span>]=<span class=\"code-snippet__number\">19<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u521b\u5efa\u4e00\u4e2a\u5411\u91cfpch\uff0c\u5176\u4e2d\u7684\u503c\u4e0ert$fustat\u4e2d\u7684\u503c\u76f8\u540c\u3002\u63a5\u7740\u6839\u636ert$fustat\u7684\u503c\uff0c\u5c06pch\u5411\u91cf\u4e2d\u4e3a1\u7684\u5143\u7d20\u8bbe\u4e3a15\uff0c\u4e3a0\u7684\u5143\u7d20\u8bbe\u4e3a19\u3002\u8fd9\u91cc\uff0c\u5c0f\u679c\u8bf4\u4e00\u4e0b\uff0cpch\u662f\u7528\u4e8e\u6307\u5b9a\u6570\u636e\u70b9\u7684\u5f62\u72b6\u54e6~<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pdf(file=<span class=\"code-snippet__string\">\"survStat.pdf\"<\/span>,width = <span class=\"code-snippet__number\">10<\/span>,height = <span class=\"code-snippet__number\">5<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u540c\u6837\u7684\uff0c\u8bbe\u7f6e\u5bbd\u548c\u9ad8\u3002\uff08\u53ef\u6839\u636e\u81ea\u5df1\u9700\u6c42\u4fee\u6539\u53c2\u6570\u54e6~\uff09<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">plot(rt$futime,<\/span><\/code><code><span class=\"code-snippet_outer\"> pch=pch,<\/span><\/code><code><span class=\"code-snippet_outer\"> xlab=<span class=\"code-snippet__string\">\"Patients (increasing risk socre)\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> ylab=<span class=\"code-snippet__string\">\"Survival time (years)\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> col=color)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u4f9d\u7136\u7528\u5230plot()\u51fd\u6570\u7ed8\u5236\u6563\u70b9\u56fe\uff0c\u6a2a\u5750\u6807\u4e3art$futime\uff0c\u7eb5\u5750\u6807\u4e3art$fustat\uff0c\u6570\u636e\u70b9\u7684\u5f62\u72b6\u7531pch\u5411\u91cf\u6307\u5b9a\uff0c\u989c\u8272\u7531color\u5411\u91cf\u6307\u5b9a\u3002\u6a2a\u5750\u6807\u548c\u7eb5\u5750\u6807\u7684\u6807\u7b7e\u5206\u522b\u4e3a<span class=\"code-snippet__meta-string\">\"Patients (increasing risk score)\"<\/span>\u548c<span class=\"code-snippet__meta-string\">\"Survival time (years)\"<\/span>\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">legend(<span class=\"code-snippet__string\">\"topleft\"<\/span>, c(<span class=\"code-snippet__string\">\"Dead\"<\/span>, <span class=\"code-snippet__string\">\"Alive\"<\/span>),bty=<span class=\"code-snippet__string\">\"n\"<\/span>,pch=c(<span class=\"code-snippet__number\">15<\/span>,<span class=\"code-snippet__number\">19<\/span>),col=c(<span class=\"code-snippet__string\">\"red\"<\/span>,<span class=\"code-snippet__string\">\"blue\"<\/span>),cex=<span class=\"code-snippet__number\">1.2<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5728\u56fe\u4e2d\u6dfb\u52a0\u56fe\u4f8b\uff0c\u4f4d\u7f6e\u5728\u5de6\u4e0a\u89d2\uff0c\u6807\u7b7e\u4e3a<span class=\"code-snippet__meta-string\">\"Dead\"<\/span>\u548c<span class=\"code-snippet__meta-string\">\"Alive\"<\/span>\uff0c\u5bf9\u5e94\u7684\u6570\u636e\u70b9\u5f62\u72b6\u4e3a15\u548c19\uff0c\u989c\u8272\u5206\u522b\u4e3a\u7ea2\u8272\u548c\u84dd\u8272\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">abline(v=lowLength,lty=<span class=\"code-snippet__number\">2<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u540c\u6837\u5728\u56fe\u4e2d\u6dfb\u52a0\u4e00\u6761\u5782\u76f4\u865a\u7ebf\uff0c\u4f4d\u7f6e\u4e3alowLength<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">dev.off()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5173\u95ed\u5e76\u4fdd\u5b58<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(0, 176, 240);font-size: 16px;\"><\/span><\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018105\" data-ratio=\"0.5059760956175299\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-04-29_\u89e3\u9501\u6570\u636e\u5965\u79d8\uff1a\u7528pheatmap\u6253\u9020\u7cbe\u7f8e\u70ed\u56fe\/5_d0UTwlCk113ZsN9oN05Bnm5WYl5DJA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAw1Y3OHHbdbv0YISSRgfb3QWXxT35osGibu6qJQd1icvysQJUd0UTwlCk113ZsN9oN05Bnm5WYl5DJA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1004\" height=\"280.20001220703125\" width=\"553.739990234375\"><\/span><\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(255, 192, 0);font-weight: bold;font-size: 16px;\"><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u98ce\u9669\u70ed\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">rt1=rt[c(3:(ncol(rt)-2))]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4ece\u6570\u636e\u6846rt\u4e2d\u9009\u53d6\u7b2c3\u5217\u5230\u5012\u6570\u7b2c3\u5217\u7684\u6570\u636e\uff0c\u5e76\u5c06\u5176\u5b58\u50a8\u5728\u65b0\u7684\u6570\u636e\u6846rt1\u4e2d\u3002\uff08\u5c0f\u4f19\u4f34\u4eec\u5982\u679c\u9009\u7528\u7684\u6570\u636e\u96c6\u548c\u5c0f\u679c\u4e0d\u540c\u7684\u8bdd\u8bb0\u5f97\u8981\u4fee\u6539\u54e6~\uff09<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">rt1=t(rt1) <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5bf9\u6570\u636e\u6846rt1\u8fdb\u884c\u8f6c\u7f6e\uff0c\u5c06\u884c\u5217\u4ea4\u6362\uff0c\u4ee5\u4fbf\u540e\u7eed\u751f\u6210\u70ed\u56fe\u65f6\u884c\u8868\u793a\u6837\u672c\uff0c\u5217\u8868\u793a\u7279\u5f81\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">rt1=log2(rt1+1)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5bf9\u6570\u636e\u6846rt1\u4e2d\u7684\u6240\u6709\u5143\u7d20\u52a01\u540e\u53d6\u5bf9\u6570\uff08\u4ee52\u4e3a\u5e95\uff09\uff0c\u5bf9\u6570\u636e\u8fdb\u884c\u5bf9\u6570\u53d8\u6362\uff0c\u4ee5\u4fbf\u66f4\u597d\u5730\u5c55\u793a\u6570\u636e\u7684\u5dee\u5f02\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">annotation=data.frame(type=rt[,ncol(rt)])<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u521b\u5efa\u4e00\u4e2a\u540d\u4e3aannotation\u7684\u6570\u636e\u6846\uff0c\u5176\u4e2d\u5305\u542b\u4e00\u4e2a\u540d\u4e3atype\u7684\u5217\uff0c\u5217\u4e2d\u7684\u503c\u6765\u81eart\u6570\u636e\u6846\u7684\u6700\u540e\u4e00\u5217\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">rownames(annotation)=rownames(rt)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c06annotation\u6570\u636e\u6846\u7684\u884c\u540d\u8bbe\u7f6e\u4e3art\u6570\u636e\u6846\u7684\u884c\u540d\uff0c\u4ee5\u4fbf\u540e\u7eed\u5728\u70ed\u56fe\u4e2d\u6dfb\u52a0\u6ce8\u91ca\u4fe1\u606f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pdf(file=<span class=\"code-snippet__string\">\"heatmap.pdf\"<\/span>,width = 10,height = 5)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u548c\u4e0a\u9762\u7684\u4e00\u6837\uff0c\u8bbe\u7f6e\u5bbd\u548c\u9ad8\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pheatmap(rt1,<\/span><\/code><code><span class=\"code-snippet_outer\"> annotation=annotation, <\/span><\/code><code><span class=\"code-snippet_outer\"> cluster_cols = FALSE,<\/span><\/code><code><span class=\"code-snippet_outer\"> cluster_rows = FALSE,<\/span><\/code><code><span class=\"code-snippet_outer\"> fontsize_row=11,<\/span><\/code><code><span class=\"code-snippet_outer\"> show_colnames = F,<\/span><\/code><code><span class=\"code-snippet_outer\"> fontsize=7,<\/span><\/code><code><span class=\"code-snippet_outer\"> fontsize_col=3,<\/span><\/code><code><span class=\"code-snippet_outer\"> color = colorRampPalette(c(<span class=\"code-snippet__string\">\"green\"<\/span>, <span class=\"code-snippet__string\">\"black\"<\/span>, <span class=\"code-snippet__string\">\"red\"<\/span>))(50) )<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7528pheatmap()\u51fd\u6570\u751f\u6210\u70ed\u56fe\uff0c\u5176\u4e2d\uff1art1\u2014\u2014\u8981\u5c55\u793a\u7684\u6570\u636e\uff1bannotation\u2014\u2014\u5305\u542b\u4e86\u6837\u672c\u7c7b\u522b\u4fe1\u606f\uff1bcluster_cols\u548ccluster_rows\u2014\u2014\u662f\u5426\u5bf9\u5217\u548c\u884c\u8fdb\u884c\u805a\u7c7b\uff0c\u8fd9\u91cc\u5c0f\u679c\u9009\u62e9\u4e86FALSE\uff0c\u5373\u751f\u6210\u70ed\u56fe\u65f6\uff0c\u884c\u548c\u5217\u7684\u987a\u5e8f\u5c06\u4fdd\u6301\u539f\u59cb\u6570\u636e\u4e2d\u7684\u987a\u5e8f\uff0c\u4e0d\u4f1a\u7ecf\u8fc7\u805a\u7c7b\u7b97\u6cd5\u91cd\u65b0\u6392\u5217\uff1bfontsize_row\u548cfontsize_col\u2014\u2014\u884c\u548c\u5217\u6807\u7b7e\u7684\u5b57\u4f53\u5927\u5c0f\uff1bshow_colnames\u2014\u2014\u662f\u5426\u663e\u793a\u5217\u540d\uff0ccolor\u2014\u2014\u6307\u5b9a\u70ed\u56fe\u7684\u989c\u8272\u6e10\u53d8\uff0c\u8fd9\u91cc\u5c0f\u679c\u4f7f\u7528\u4e86\u4ece\u7eff\u8272\u5230\u7ea2\u8272\u7684\u6e10\u53d8\uff0c\u517150\u4e2a\u989c\u8272\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">dev.off()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5173\u95ed\u5e76\u4fdd\u5b58<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(0, 176, 240);font-size: 16px;\"><\/span><\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(0, 176, 240);font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018107\" data-ratio=\"0.5045500505561172\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-04-29_\u89e3\u9501\u6570\u636e\u5965\u79d8\uff1a\u7528pheatmap\u6253\u9020\u7cbe\u7f8e\u70ed\u56fe\/6_Swd7sZ5Y6Zn3YaGsxCbhqbHVibwkWw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAw1Y3OHHbdbv0YISSRgfb3QsvgIyicF0jFdIChUv6aJdicLnH8Swd7sZ5Y6Zn3YaGsxCbhqbHVibwkWw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"989\" height=\"279.4000244140625\" width=\"553.739990234375\" \/><\/span><\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(0, 176, 240);font-size: 16px;\">          <o:p> <\/o:p><\/span><\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;\">\u98ce\u9669\u66f2\u7ebf\u3001\u751f\u5b58\u72b6\u6001\u56fe\u4e0e\u98ce\u9669\u70ed\u56fe\uff0c\u65e0\u7591\u662f\u751f\u4fe1\u9886\u57df\u7684\u7480\u74a8\u660e\u73e0\uff0c\u5b83\u4eec\u76f4\u89c2\u800c\u6df1\u523b\u5730\u63ed\u793a\u4e86\u751f\u7269\u6570\u636e\u4e2d\u7684\u98ce\u9669\u5965\u79d8\u3002\u901a\u8fc7\u5b83\u4eec\uff0c\u6211\u4eec\u80fd\u591f\u6d1e\u5bdf\u75be\u75c5\u7684\u8fdb\u5c55\u8109\u7edc\uff0c\u628a\u63e1\u98ce\u9669\u5206\u5e03\u4e0e\u53d8\u5316\u8d8b\u52bf\uff0c\u4e3a\u75be\u75c5\u7684\u9884\u9632\u3001\u8bca\u65ad\u548c\u6cbb\u7597\u94fa\u8bbe\u575a\u5b9e\u7684\u57fa\u77f3\u3002<\/span><\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u672c\u671f\uff0c\u5c0f\u679c\u4e0d\u4ec5\u4e3a\u5c0f\u4f19\u4f34\u4eec\u5e26\u6765\u4e86pheatmap\u5305\u5982\u4f55\u7ed8\u5236\u51fa\u7cbe\u7f8e\u7edd\u4f26\u7684\u70ed\u56fe\u540c\u65f6\uff0c\u8fd8\u4ee5\u75be\u75c5\u8fdb\u5c55\u4e3a\u5b9e\u4f8b\uff0c\u5c55\u793a\u4e86\u5176\u5b9e\u9645\u5e94\u7528\u3002\u5e0c\u671b\u5bf9\u5c0f\u4f19\u4f34\u4eec\u6709\u6240\u88e8\u76ca~\u4e0b\u671f\u518d\u89c1\u54e6\uff0c\u671f\u5f85\u4e0e\u5c0f\u4f19\u4f34\u4eec\u7ee7\u7eed\u76f8\u7ea6\uff0c\u4e00\u8d77\u63a2\u5bfb\u751f\u4fe1\u4e4b\u7f8e\uff01<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(255, 0, 0);font-weight: bold;\">\u5982\u679c\u5404\u4f4d\u89c9\u5f97\u81ea\u5df1\u8fd0\u884c\u4ee3\u7801\u592a\u9ebb\u70e6\uff0c\u6b22\u8fce\u7528\u6211\u4eec\u7684\u4e91\u751f\u4fe1\u5c0f\u5de5\u5177\uff0c\u53ea\u8981\u8f93\u5165\u5408\u9002\u7684\u6570\u636e\u5c31\u53ef\u4ee5\u76f4\u63a5\u51fa\u60f3\u8981\u7684\u56fe\u5462\uff0c\u9644\u4e91\u751f\u4fe1\u94fe\u63a5<\/span><\/span><\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(255, 0, 0);font-weight: bold;\">\uff08http:\/\/www.biocloudservice.com\/home.html\uff09<\/span><\/span><\/section>\n<section style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;\">  <\/span><\/section>\n<p style=\"text-indent: 0px;margin-bottom: 0px;\"><span style=\"color: rgb(0, 122, 170);\"><strong><span style=\"color: rgb(0, 122, 170);letter-spacing: 0.578px;text-indent: 32px;text-wrap: wrap;background-color: rgb(255, 255, 255);font-size: 15px;\">\u5c0f\u679c\u8fd8<span style=\"color: rgb(0, 122, 170);background-color: rgb(255, 255, 255);font-size: 15px;letter-spacing: 0.578px;text-decoration: none solid rgba(0, 0, 0, 0.9);\">\u63d0\u4f9b\u601d\u8def\u8bbe\u8ba1\u3001\u5b9a\u5236\u751f\u4fe1\u5206\u6790\u3001\u6587\u732e\u601d\u8def\u590d\u73b0\uff1b\u6709\u9700\u8981\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u76f4\u63a5\u626b\u7801\u54a8\u8be2\u5c0f\u679c\uff0c\u7aed\u8bda\u4e3a\u60a8\u7684\u79d1\u7814\u52a9\u529b\uff01<\/span><\/span><\/strong><\/span><\/p>\n<p style=\"margin-bottom: 0px;\"><br \/><\/p>\n<section style=\"font-size: 15px;padding-right: 2px;padding-left: 2px;margin-bottom: 0px;\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;flex: 0 0 auto;height: auto;border-color: rgb(114, 114, 114);border-width: 1px;border-style: solid;box-shadow: rgb(194, 209, 255) -5px 5px 0px;align-self: flex-start;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 42.6829%;vertical-align: middle;box-shadow: rgb(43, 65, 110) 0px 0px 0px;flex: 0 0 auto;height: auto;padding-left: 10px;align-self: center;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;line-height: 0;transform: translate3d(20px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 75%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018110\" data-ratio=\"1\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-04-29_\u89e3\u9501\u6570\u636e\u5965\u79d8\uff1a\u7528pheatmap\u6253\u9020\u7cbe\u7f8e\u70ed\u56fe\/7_B2jkicacLzPjw4xMSqVvoucZjonVDA.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxVYfSsM0wdf9d3RpNiaTcHohxPNEVs2VFhPMukpU8lchxrvcB2jkicacLzPjw4xMSqVvoucZjonVDA\/640?wx_fmt=jpeg&from=appmsg'\" data-type=\"jpeg\" data-w=\"430\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 56.9106%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"line-height: 2;letter-spacing: 1px;color: rgb(43, 65, 110);\" powered-by=\"xiumi.us\">\n<p style=\"margin-bottom: 2px;\"><strong>\u5b9a\u5236\u751f\u4fe1\u5206\u6790<\/strong><\/p>\n<p style=\"margin-bottom: 2px;\"><strong>\u670d\u52a1\u5668\u79df\u8d41<\/strong><\/p>\n<p><strong>\u626b\u7801\u54a8\u8be2\u5c0f\u679c<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247497780&idx=2&sn=7eee90af6f387e4153bba13028e545a1&chksm=cf7f68f1f808e1e7dfb3a0597ef442e5c6e700ecf6ee5a035c766a4c7486c12eabd0ab218ea6&scene=21#wechat_redirect\" textvalue=\"\u4f60\u5df2\u9009\u4e2d\u4e86\u6dfb\u52a0\u94fe\u63a5\u7684\u5185\u5bb9\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-imgfileid=\"100018112\" data-ratio=\"0.3253333333333333\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-04-29_\u89e3\u9501\u6570\u636e\u5965\u79d8\uff1a\u7528pheatmap\u6253\u9020\u7cbe\u7f8e\u70ed\u56fe\/8_7TvAyUibd1TPhJmZfialJtv6tu2SEg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxVYfSsM0wdf9d3RpNiaTcHoj9xlsoyiaBvzaezDUFAicibMAF8ts7TvAyUibd1TPhJmZfialJtv6tu2SEg\/640?wx_fmt=png&from=appmsg'\" data-type=\"png\" data-w=\"750\" style=\"vertical-align: middle;width: 100%;\"><\/span><\/a><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><br \/><\/section>\n<\/section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;text-align: left;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-end;padding-right: 2px;padding-left: 2px;\">\n<section style=\"margin: 10px 0% 2px;transform: translate3d(2px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 16px;color: rgb(58, 66, 94);line-height: 1;letter-spacing: 5px;text-align: center;\">\n<p><strong>\u5f80\u671f\u56de\u987e<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"height: 3px;background-color: rgb(95, 156, 239);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: 2px;margin-right: 0%;margin-left: 0%;text-align: center;line-height: 0.8;font-size: 9px;\" powered-by=\"xiumi.us\">\n<section style=\"width: 0px;display: inline-block;border-top: 0.8em solid rgb(58, 66, 94);border-left: 0.7em solid transparent !important;border-right: 0.7em solid transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;flex: 100 100 0%;align-self: flex-end;height: auto;\">\n<section style=\"margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"height: 3px;background-color: rgb(95, 156, 239);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: 2px;margin-right: 0%;margin-left: 0%;text-align: center;line-height: 0.8;font-size: 9px;\" powered-by=\"xiumi.us\">\n<section style=\"width: 0px;display: inline-block;border-top: 0.8em solid rgba(255, 255, 255, 0);border-left: 0.7em solid transparent !important;border-right: 0.7em solid transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"min-height: 40px;margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"width: 100%;margin-right: auto;margin-bottom: -10px;margin-left: auto;\">\n<table width=\"100%\">\n<tbody>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:0\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:0.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;box-shadow: rgb(0, 0, 0) 0px 0px 0px;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>01<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247497780&idx=2&sn=7eee90af6f387e4153bba13028e545a1&chksm=cf7f68f1f808e1e7dfb3a0597ef442e5c6e700ecf6ee5a035c766a4c7486c12eabd0ab218ea6&scene=21#wechat_redirect\" textvalue=\"1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff0c\u4e24\u4eba\u6210\u56e2\uff0c1\u4eba\u514d\u5355\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff0c\u4e24\u4eba\u6210\u56e2\uff0c1\u4eba\u514d\u5355\uff01<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:1\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:1.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>02<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247498878&idx=1&sn=fc34ed3f1d15d060bbaf8166adcebd58&chksm=cf7f6cbbf808e5ad0b5417e50ed75cfbd1b3a83ac43b97f1dfa718c822878a9a004990075133&scene=21#wechat_redirect\" textvalue=\"\u5355\u4e2a\u6570\u636e\u5e93\u7528\u817b\u4e86\uff1f\u591a\u6570\u636e\u5e93\u201c\u7ec4\u5408\u62f3\u201d\u5e26\u4f60\u6253\u5f00\u514d\u75ab\u6d78\u6da6\u65b0\u601d\u8def\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u5355\u4e2a\u6570\u636e\u5e93\u7528\u817b\u4e86\uff1f\u591a\u6570\u636e\u5e93\u201c\u7ec4\u5408\u62f3\u201d\u5e26\u4f60\u6253\u5f00\u514d\u75ab\u6d78\u6da6\u65b0\u601d\u8def\uff01<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:2\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:2.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>03<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247498673&idx=1&sn=1e09b74a836c1e64a62ae1f66eeb3f93&chksm=cf7f6b74f808e262de70be43fd73bf5fefdc0914e525eb2032f741a0152e619689cedc76946f&scene=21#wechat_redirect\" textvalue=\"\u5b5f\u5fb7\u5c14\u968f\u673a\u5316\u7684\u51c6\u5907\u5de5\u4f5c\uff0cGWAS\u6570\u636e\u7684\u7f51\u7ad9\u4e0b\u8f7d\u65b9\u6cd5\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"11\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u5b5f\u5fb7\u5c14\u968f\u673a\u5316\u7684\u51c6\u5907\u5de5\u4f5c\uff0cGWAS\u6570\u636e\u7684\u7f51\u7ad9\u4e0b\u8f7d\u65b9\u6cd5<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:3\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:3.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>04<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" 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