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onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAyB69UyXDLG0wQcISNwmL6DlpIKVBnCSOXHTWJhLcxic2qtibaib2l1ye6HNIkwTG696C2pf9yAtdeGA\/640?wx_fmt=png'\" data-type=\"jpeg\" data-ratio=\"1\" data-w=\"400\" style=\"height: 111px;vertical-align: middle;width: 111px;\" \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section data-role=\"list\">\n<section style=\"font-size: 16px;\">\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"margin: 10px 0% 8px;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;border-left: 3px solid rgb(219, 219, 219);border-bottom-left-radius: 0px;padding-left: 8px;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"color: rgba(0, 0, 0, 0.5);text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u6709\u65f6\u5019\u6211\u4eec\u60f3\u8981\u4e0b\u8f7d\u522b\u4eba\u7684\u8f6c\u5f55\u7ec4\u6570\u636e\u8fdb\u884c\u7ec3\u4e60\u6216\u5206\u6790\uff0c\u53ef\u80fd\u4f1a\u7a81\u7136\u53d1\u73b0\u65e0\u4ece\u4e0b\u624b\uff0c\u5de7\u5987\u96be\u4e3a\u65e0\u7c73\u4e4b\u708a\u554a\uff0c\u8fd9\u53ef\u600e\u4e48\u529e\uff1f<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5c0f\u679c\u672c\u671f\u6765\u5206\u4eab\u7528SRA Toolkit\u5de5\u5177\u5728NCBI\u7684SRA\u6570\u636e\u5e93\u4e0b\u8f7d\u62df\u5357\u82a5\u7684\u8f6c\u5f55\u7ec4\u6570\u636eRun Selector :: NCBI (nih.gov)\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">SRA Toolkit\u662f\u7531\u7f8e\u56fd\u56fd\u5bb6\u751f\u7269\u6280\u672f\u4fe1\u606f\u4e2d\u5fc3\uff08NCBI\uff09\u5f00\u53d1\u7684\u4e00\u6b3e\u7528\u4e8e\u9ad8\u901a\u91cf\u6d4b\u5e8f\u6570\u636e\u5904\u7406\u7684\u8f6f\u4ef6\u5305\uff0c\u4e3b\u8981\u7528\u4e8e\u5b58\u50a8\u548c\u5206\u6790NCBI Sequence Read Archive\uff08SRA\uff09\u4e2d\u7684\u6d4b\u5e8f\u6570\u636e\u3002\u8be5\u5de5\u5177\u5305\u63d0\u4f9b\u4e86\u591a\u4e2a\u547d\u4ee4\u884c\u5de5\u5177\uff0c\u652f\u6301\u4eceSRA\u4e0b\u8f7d\u6570\u636e\u3001\u5c06SRA\u8f6c\u6362\u4e3a\u5e38\u89c1\u7684FASTQ\u683c\u5f0f\u3001\u5c06FASTQ\u6587\u4ef6\u8f6c\u6362\u4e3aSRA\u683c\u5f0f\u7b49\u591a\u79cd\u529f\u80fd\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">SRA Toolkit\u4e0d\u4ec5\u9002\u7528\u4e8eNCBI SRA\u6570\u636e\u5e93\uff0c\u4e5f\u53ef\u7528\u4e8e\u5904\u7406\u5176\u4ed6\u6765\u6e90\u7684\u9ad8\u901a\u91cf\u6d4b\u5e8f\u6570\u636e\u3002\u4f7f\u7528SRA Toolkit\u53ef\u4ee5\u5b9e\u73b0\u9ad8\u6548\u7684\u6570\u636e\u4e0b\u8f7d\u3001\u6570\u636e\u683c\u5f0f\u8f6c\u6362\u3001\u6570\u636e\u5904\u7406\u548c\u5206\u6790\u7b49\u591a\u4e2a\u6b65\u9aa4\uff0c\u5e76\u4e14\u652f\u6301\u591a\u7ebf\u7a0b\u548c\u5206\u5e03\u5f0f\u5904\u7406\uff0c\u80fd\u591f\u52a0\u5feb\u6570\u636e\u5904\u7406\u901f\u5ea6\u548c\u63d0\u9ad8\u6570\u636e\u5904\u7406\u6548\u7387\u3002\u4e0b\u9762\u6211\u4eec\u4e00\u8d77\u6765\u770b\u770b\u8fd9\u4e2a\u5de5\u5177\u5982\u4f55\u4f7f\u7528\u5427~<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;\">1. \u5b89\u88c5SRA Toolkit<\/span><\/em><\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">conda create --name sratoolkit<\/span><\/code><code><span class=\"code-snippet_outer\">conda activate sratoolkit<\/span><\/code><code><span class=\"code-snippet_outer\">conda install -c bioconda sra-tools<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;\">2.\u4e0b\u8f7d\u6570\u636els<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u6fc0\u6d3bsratollkit\u73af\u5883\uff0c\u7136\u540e\u5c31\u53ef\u4ee5\u7528\u5b83\u6279\u91cf\u4e0b\u8f7d\u6570\u636e\u4e86\uff0c\u56e0\u4e3a\u6570\u636e\u91cf\u592a\u5927\uff0c\u800c\u5c0f\u679c\u53ea\u662f\u60f3\u4f5c\u4e3a\u7ec3\u4e60\uff0c\u56e0\u6b64\u53ea\u4e0b\u8f7d\u4e869\u4e2a \u6837\u672c\u7684\u6d4b\u5e8f\u6570\u636e\uff0c\u5177\u4f53\u65b9\u6cd5\u5982\u4e0b\uff1a<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\uff081\uff09\u67e5\u627e\u9700\u8981\u4e0b\u8f7d\u7684SRA\u6570\u636e\u7684\u8bbf\u95ee\u53f7\uff08Accession Number\uff09<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\uff082\uff09\u4e0b\u8f7dAccession List\uff0c\u4f1a\u4e0b\u8f7d\u4e00\u4e2a\u5305\u542b\u6240\u6709Run\u7f16\u53f7\u7684\u6587\u672c\u6587\u6863<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.16064981949458484\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_T2YGtXx2cNicGsD3eTJPYDJP3ib7Gw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxQSA3GFmsGcUB5nTcvictPSibQso0rbDGW1p1TrvUDS34KAkW8T2YGtXx2cNicGsD3eTJPYDJP3ib7Gw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.25753424657534246\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_fV2yF28qcMk6w6FXWtlmYvq4JVsEjg.png\" 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inline-block;\">\n<section style=\"padding: 7px 15px;border-radius: 20px;box-shadow: rgba(169, 187, 223, 0.45) 6px 6px 8px inset, rgb(255, 255, 255) -2.5px -2.5px 5px inset;\">\n<section data-brushtype=\"text\" style=\"font-size: 14px;letter-spacing: 1.5px;color: rgb(111, 133, 172);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u5fae\u4fe1\u53f7 | 18502195490<\/span><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-top: 12px;padding: 7px 15px;border-radius: 20px;box-shadow: rgba(169, 187, 223, 0.45) 6px 6px 8px inset, rgb(255, 255, 255) -2.5px -2.5px 5px inset;\">\n<section data-brushtype=\"text\" style=\"font-size: 14px;letter-spacing: 1.5px;color: rgb(111, 133, 172);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u77e5\u4e4e | \u751f\u4fe1\u679c<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-top: 0.5em;\">\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);\">\n<section style=\"text-align: left;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;\">\n<section style=\"margin-bottom: 8px;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u70b9\u51fb<strong><span style=\"color: rgb(0, 150, 136);\">\u201c\u9605\u8bfb\u539f\u6587\u201d<\/span><\/strong>\u7acb\u523b\u62e5\u6709<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 136);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u2193\u2193\u2193 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solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"\u5c0f\u679c\u53d1\u73b0\u7528SRA Toolkit\u5de5\u5177\u4e0b\u8f7d\u8f6c\u5f55\u7ec4\u6570\u636e\u5f88\u597d\u7528\uff01\",\"time\":\"2023-06-04 19:00:26\",\"timeStamp\":\"1685876426\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247488013&idx=1&sn=66b12d1f918b4d97c52adb16ab1b8cd7&chksm=cf7c82c8f80b0bde41620da711d3f8643b33e99b544e29b003a310691dc5d49b16ac99075616#rd\" target=\"_blank\" rel=\"noopener\">\u5c0f\u679c\u53d1\u73b0\u7528SRA Toolkit\u5de5\u5177\u4e0b\u8f7d\u8f6c\u5f55\u7ec4\u6570\u636e\u5f88\u597d\u7528\uff01<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span id=\"copyright_logo\" class=\"wx_tap_link js_wx_tap_highlight rich_media_meta icon_appmsg_tag appmsg_title_tag weui-wa-hotarea\">\u539f\u521b <\/span><span class=\"rich_media_meta rich_media_meta_text\">xiao?guo 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15px;\">\u5f00\u542f\u5347\u7ea7\u6a21\u5f0f\u5427<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"color: rgb(255, 129, 36);font-size: 12px;letter-spacing: 0.5px;\">\uff0856\u7ebf\u7a0b\uff0c256G\u5185\u5b58\uff0c\u4e2a\u4eba\u5b58\u50a81T\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;width: 172.594px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"162\" data-cropsely1=\"0\" data-cropsely2=\"162\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_ib2l1ye6HNIkwTG696C2pf9yAtdeGA-32.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAyB69UyXDLG0wQcISNwmL6DlpIKVBnCSOXHTWJhLcxic2qtibaib2l1ye6HNIkwTG696C2pf9yAtdeGA\/640?wx_fmt=png'\" data-type=\"jpeg\" data-ratio=\"1\" data-w=\"400\" style=\"height: 111px;vertical-align: middle;width: 111px;\" \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section data-role=\"list\">\n<section style=\"font-size: 16px;\">\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"margin: 10px 0% 8px;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;border-left: 3px solid rgb(219, 219, 219);border-bottom-left-radius: 0px;padding-left: 8px;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"color: rgba(0, 0, 0, 0.5);text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u6709\u65f6\u5019\u6211\u4eec\u60f3\u8981\u4e0b\u8f7d\u522b\u4eba\u7684\u8f6c\u5f55\u7ec4\u6570\u636e\u8fdb\u884c\u7ec3\u4e60\u6216\u5206\u6790\uff0c\u53ef\u80fd\u4f1a\u7a81\u7136\u53d1\u73b0\u65e0\u4ece\u4e0b\u624b\uff0c\u5de7\u5987\u96be\u4e3a\u65e0\u7c73\u4e4b\u708a\u554a\uff0c\u8fd9\u53ef\u600e\u4e48\u529e\uff1f<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5c0f\u679c\u672c\u671f\u6765\u5206\u4eab\u7528SRA Toolkit\u5de5\u5177\u5728NCBI\u7684SRA\u6570\u636e\u5e93\u4e0b\u8f7d\u62df\u5357\u82a5\u7684\u8f6c\u5f55\u7ec4\u6570\u636eRun Selector :: NCBI (nih.gov)\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">SRA Toolkit\u662f\u7531\u7f8e\u56fd\u56fd\u5bb6\u751f\u7269\u6280\u672f\u4fe1\u606f\u4e2d\u5fc3\uff08NCBI\uff09\u5f00\u53d1\u7684\u4e00\u6b3e\u7528\u4e8e\u9ad8\u901a\u91cf\u6d4b\u5e8f\u6570\u636e\u5904\u7406\u7684\u8f6f\u4ef6\u5305\uff0c\u4e3b\u8981\u7528\u4e8e\u5b58\u50a8\u548c\u5206\u6790NCBI Sequence Read Archive\uff08SRA\uff09\u4e2d\u7684\u6d4b\u5e8f\u6570\u636e\u3002\u8be5\u5de5\u5177\u5305\u63d0\u4f9b\u4e86\u591a\u4e2a\u547d\u4ee4\u884c\u5de5\u5177\uff0c\u652f\u6301\u4eceSRA\u4e0b\u8f7d\u6570\u636e\u3001\u5c06SRA\u8f6c\u6362\u4e3a\u5e38\u89c1\u7684FASTQ\u683c\u5f0f\u3001\u5c06FASTQ\u6587\u4ef6\u8f6c\u6362\u4e3aSRA\u683c\u5f0f\u7b49\u591a\u79cd\u529f\u80fd\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">SRA Toolkit\u4e0d\u4ec5\u9002\u7528\u4e8eNCBI SRA\u6570\u636e\u5e93\uff0c\u4e5f\u53ef\u7528\u4e8e\u5904\u7406\u5176\u4ed6\u6765\u6e90\u7684\u9ad8\u901a\u91cf\u6d4b\u5e8f\u6570\u636e\u3002\u4f7f\u7528SRA Toolkit\u53ef\u4ee5\u5b9e\u73b0\u9ad8\u6548\u7684\u6570\u636e\u4e0b\u8f7d\u3001\u6570\u636e\u683c\u5f0f\u8f6c\u6362\u3001\u6570\u636e\u5904\u7406\u548c\u5206\u6790\u7b49\u591a\u4e2a\u6b65\u9aa4\uff0c\u5e76\u4e14\u652f\u6301\u591a\u7ebf\u7a0b\u548c\u5206\u5e03\u5f0f\u5904\u7406\uff0c\u80fd\u591f\u52a0\u5feb\u6570\u636e\u5904\u7406\u901f\u5ea6\u548c\u63d0\u9ad8\u6570\u636e\u5904\u7406\u6548\u7387\u3002\u4e0b\u9762\u6211\u4eec\u4e00\u8d77\u6765\u770b\u770b\u8fd9\u4e2a\u5de5\u5177\u5982\u4f55\u4f7f\u7528\u5427~<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;\">1. \u5b89\u88c5SRA Toolkit<\/span><\/em><\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">conda create --name sratoolkit<\/span><\/code><code><span class=\"code-snippet_outer\">conda activate sratoolkit<\/span><\/code><code><span class=\"code-snippet_outer\">conda install -c bioconda sra-tools<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;\">2.\u4e0b\u8f7d\u6570\u636els<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u6fc0\u6d3bsratollkit\u73af\u5883\uff0c\u7136\u540e\u5c31\u53ef\u4ee5\u7528\u5b83\u6279\u91cf\u4e0b\u8f7d\u6570\u636e\u4e86\uff0c\u56e0\u4e3a\u6570\u636e\u91cf\u592a\u5927\uff0c\u800c\u5c0f\u679c\u53ea\u662f\u60f3\u4f5c\u4e3a\u7ec3\u4e60\uff0c\u56e0\u6b64\u53ea\u4e0b\u8f7d\u4e869\u4e2a \u6837\u672c\u7684\u6d4b\u5e8f\u6570\u636e\uff0c\u5177\u4f53\u65b9\u6cd5\u5982\u4e0b\uff1a<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\uff081\uff09\u67e5\u627e\u9700\u8981\u4e0b\u8f7d\u7684SRA\u6570\u636e\u7684\u8bbf\u95ee\u53f7\uff08Accession Number\uff09<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\uff082\uff09\u4e0b\u8f7dAccession List\uff0c\u4f1a\u4e0b\u8f7d\u4e00\u4e2a\u5305\u542b\u6240\u6709Run\u7f16\u53f7\u7684\u6587\u672c\u6587\u6863<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.16064981949458484\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_T2YGtXx2cNicGsD3eTJPYDJP3ib7Gw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxQSA3GFmsGcUB5nTcvictPSibQso0rbDGW1p1TrvUDS34KAkW8T2YGtXx2cNicGsD3eTJPYDJP3ib7Gw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.25753424657534246\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_fV2yF28qcMk6w6FXWtlmYvq4JVsEjg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxQSA3GFmsGcUB5nTcvictPSKXC0vE1zfDdLEnlcS14LA1AyfV2yF28qcMk6w6FXWtlmYvq4JVsEjg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"365\" style=\"\" \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5c0f\u679c\u8fd9\u91cc\u53ea\u4fdd\u7559\u4e869\u4e2a\u6837\u672c\uff08\u5728\u6587\u6863\u91cc\u5220\u9664\u5176\u4ed6\u4e0d\u9700\u8981\u7684run\u7f16\u53f7\u5c31\u53ef\u4ee5\u4e86\uff09\uff0c\u9700\u8981\u7684\u8bdd\u53ef\u4ee5\u5168\u90e8\u4e0b\u8f7d\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\uff083\uff09\u5728\u7ec8\u7aef\u4e2d\u5207\u6362\u5230\u8981\u5c06SRA\u6587\u4ef6\u4e0b\u8f7d\u5230\u7684\u76ee\u5f55<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">nohup prefetch -O . $(<srr_acc_list.txt) &<=”” span=””>###\u5c06\u6587\u4ef6\u4e0b\u8f7d\u5230\u5f53\u524d\u76ee\u5f55\u5e76\u4f5c\u4e3a\u540e\u53f0\u8fdb\u7a0b\u8fd0\u884c<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.509375\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_rFt3Qj0NLBVMBwtz8qpIPx8iaSMElA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxQSA3GFmsGcUB5nTcvictPSfg7xTwWn7zJgVmF4681rqJySJrFt3Qj0NLBVMBwtz8qpIPx8iaSMElA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"320\" style=\"\" \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.370253164556962\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_dp41gkYfOCkMGMmL2HSianiabMoJ0g.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxQSA3GFmsGcUB5nTcvictPS7PmJqBpJKXiaHGQ7csTrsIBYSwQdp41gkYfOCkMGMmL2HSianiabMoJ0g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"316\" style=\"\" \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5f53\u7136\u4e86\uff0c\u4f60\u4e5f\u53ef\u4ee5\u8fd9\u6837\u4e0b\u8f7d\uff0c\u4e0d\u8fc7\u4f1a\u6bd4\u8f83\u9ebb\u70e6\uff1a<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">prefetch SRR8054374 SRR8054375 SRR8054376 SRR8054377 SRR8054378 SRR8054379 SRR8054380 SRR8054381 SRR8054382### SRR8054374\u7b49\u4e3aSRA\u6587\u4ef6\u7684run number<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">a year later\u2026\u2026<\/span><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u597d\u5566\uff0c\u53ef\u4ee5\u770b\u5230\u6587\u4ef6\u5df2\u7ecf\u4e0b\u8f7d\u5b8c\u6210\u4e86\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;\">3.\u8f6c\u6362\u683c\u5f0f<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5982\u679c\u4f60\u4e0b\u8f7d\u7684\u6587\u4ef6\u5728\u540c\u4e00\u4e2a\u76ee\u5f55\u4e0b\uff1a<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">fastq-dump --gzip --split-files *.sra &#\u5c06.sra\u6587\u4ef6\u8f6c\u6362\u4e3a.fastq.gz\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\">gunzip *.fastq.gz #\u89e3\u538b\u7f29\u6240\u6709.fastq.gz\u6587\u4ef6<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4ee5\u4e0a\u5c31\u662f\u5168\u90e8\u5185\u5bb9\u5566\uff0c\u6211\u4eec\u4e0b\u671f\u89c1\uff01<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> 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space-evenly;align-items: center;\">\n<section data-width=\"26%\" style=\"width: 150.266px;\">\n<section style=\"padding: 7px;border-radius: 15px;box-shadow: rgba(169, 187, 223, 0.45) 4px 4px 8px, rgb(255, 255, 255) -2.5px -2.5px 5px;\">\n<section data-width=\"100%\" style=\"width: 136.266px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.005281690140845\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_0WFLKBMicbm6iawtkrNrMxpSVU9GBQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtN261llpmfF6btPBTUKkibEdnRz5Y0WFLKBMicbm6iawtkrNrMxpSVU9GBQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"568\" data-width=\"100%\" style=\"vertical-align: inherit;width: 136.266px;display: block;border-radius: 7px;\" title=\"\u5c0f\u4e91-\u5fae\u4fe1\u4e8c\u7ef4\u7801.png\" \/><\/section>\n<\/section>\n<\/section>\n<section data-width=\"62%\" style=\"width: 358.359px;\">\n<section style=\"display: inline-block;\">\n<section style=\"padding: 7px 15px;border-radius: 20px;box-shadow: rgba(169, 187, 223, 0.45) 6px 6px 8px inset, rgb(255, 255, 255) -2.5px -2.5px 5px inset;\">\n<section data-brushtype=\"text\" style=\"font-size: 14px;letter-spacing: 1.5px;color: rgb(111, 133, 172);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u5fae\u4fe1\u53f7 | 18502195490<\/span><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-top: 12px;padding: 7px 15px;border-radius: 20px;box-shadow: rgba(169, 187, 223, 0.45) 6px 6px 8px inset, rgb(255, 255, 255) -2.5px -2.5px 5px inset;\">\n<section data-brushtype=\"text\" style=\"font-size: 14px;letter-spacing: 1.5px;color: rgb(111, 133, 172);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u77e5\u4e4e | \u751f\u4fe1\u679c<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-top: 0.5em;\">\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);\">\n<section style=\"text-align: left;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;\">\n<section style=\"margin-bottom: 8px;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u70b9\u51fb<strong><span style=\"color: rgb(0, 150, 136);\">\u201c\u9605\u8bfb\u539f\u6587\u201d<\/span><\/strong>\u7acb\u523b\u62e5\u6709<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 136);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u2193\u2193\u2193 <\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5c0f\u679c\u53d1\u73b0\u7528SRA Toolkit\u5de5\u5177\u4e0b\u8f7d\u8f6c\u5f55\u7ec4\u6570\u636e\u5f88\u597d\u7528\uff01 \u539f\u521b xiao?guo&nb […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55732"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=55732"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55732\/revisions"}],"predecessor-version":[{"id":55739,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55732\/revisions\/55739"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=55732"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=55732"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=55732"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}